LOCUS       DADLEE010000001        96391 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000001 DADLEE010000000
VERSION     DADLEE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 96391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96391
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.001"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            288..632
                     /locus_tag="JQH51_000001"
     CDS             288..632
                     /locus_tag="JQH51_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY6074511.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            655..1026
                     /locus_tag="JQH51_000002"
     CDS             655..1026
                     /locus_tag="JQH51_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY6074512.1"
                     /translation="MTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(1078..2493)
                     /gene="gltX"
                     /locus_tag="JQH51_000003"
     CDS             complement(1078..2493)
                     /gene="gltX"
                     /locus_tag="JQH51_000003"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY6074513.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            2752..2827
                     /locus_tag="JQH51_000004"
     tRNA            2752..2827
                     /locus_tag="JQH51_000004"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2785..2787,aa:Val,seq:tac)
     gene            2872..2947
                     /locus_tag="JQH51_000005"
     tRNA            2872..2947
                     /locus_tag="JQH51_000005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2905..2907,aa:Val,seq:tac)
     gene            2952..3027
                     /locus_tag="JQH51_000006"
     tRNA            2952..3027
                     /locus_tag="JQH51_000006"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2985..2987,aa:Lys,seq:ttt)
     gene            3058..3183
                     /locus_tag="JQH51_000007"
     ncRNA           3058..3183
                     /ncRNA_class="other"
                     /locus_tag="JQH51_000007"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            3303..3644
                     /locus_tag="JQH51_000008"
     CDS             3303..3644
                     /locus_tag="JQH51_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY6074514.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(3635..4561)
                     /locus_tag="JQH51_000009"
     CDS             complement(3635..4561)
                     /locus_tag="JQH51_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6074515.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4651..5649
                     /locus_tag="JQH51_000010"
     CDS             4651..5649
                     /locus_tag="JQH51_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY6074516.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5646..5864)
                     /locus_tag="JQH51_000011"
     CDS             complement(5646..5864)
                     /locus_tag="JQH51_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074517.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPTG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5866..7881)
                     /gene="ligA"
                     /locus_tag="JQH51_000012"
     CDS             complement(5866..7881)
                     /gene="ligA"
                     /locus_tag="JQH51_000012"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY6074518.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7952..8938)
                     /gene="zipA"
                     /locus_tag="JQH51_000013"
     CDS             complement(7952..8938)
                     /gene="zipA"
                     /locus_tag="JQH51_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY6074519.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9168..9929
                     /gene="cysZ"
                     /locus_tag="JQH51_000014"
     CDS             9168..9929
                     /gene="cysZ"
                     /locus_tag="JQH51_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY6074520.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10114..11085
                     /gene="cysK"
                     /locus_tag="JQH51_000015"
     CDS             10114..11085
                     /gene="cysK"
                     /locus_tag="JQH51_000015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY6074521.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11469..11726
                     /gene="ptsH"
                     /locus_tag="JQH51_000016"
     CDS             11469..11726
                     /gene="ptsH"
                     /locus_tag="JQH51_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY6074522.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11771..13498
                     /gene="ptsI"
                     /locus_tag="JQH51_000017"
     CDS             11771..13498
                     /gene="ptsI"
                     /locus_tag="JQH51_000017"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY6074523.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13539..14048
                     /gene="crr"
                     /locus_tag="JQH51_000018"
     CDS             13539..14048
                     /gene="crr"
                     /locus_tag="JQH51_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY6074524.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14090..14941)
                     /gene="pdxK"
                     /locus_tag="JQH51_000019"
                     /gene_synonym="thiJ"
     CDS             complement(14090..14941)
                     /gene="pdxK"
                     /locus_tag="JQH51_000019"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY6074525.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15046..15420
                     /locus_tag="JQH51_000020"
     CDS             15046..15420
                     /locus_tag="JQH51_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY6074526.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            15453..16176
                     /locus_tag="JQH51_000021"
                     /pseudo
     CDS             15453..16176
                     /locus_tag="JQH51_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16380..17291)
                     /gene="cysM"
                     /locus_tag="JQH51_000022"
     CDS             complement(16380..17291)
                     /gene="cysM"
                     /locus_tag="JQH51_000022"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY6074527.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17425..18522)
                     /gene="cysA"
                     /locus_tag="JQH51_000023"
     CDS             complement(17425..18522)
                     /gene="cysA"
                     /locus_tag="JQH51_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY6074528.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18512..19387)
                     /gene="cysW"
                     /locus_tag="JQH51_000024"
     CDS             complement(18512..19387)
                     /gene="cysW"
                     /locus_tag="JQH51_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY6074529.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19387..20220)
                     /gene="cysT"
                     /locus_tag="JQH51_000025"
     CDS             complement(19387..20220)
                     /gene="cysT"
                     /locus_tag="JQH51_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY6074530.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20220..21236)
                     /locus_tag="JQH51_000026"
     CDS             complement(20220..21236)
                     /locus_tag="JQH51_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6074531.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(21540..22331)
                     /gene="ucpA"
                     /locus_tag="JQH51_000027"
     CDS             complement(21540..22331)
                     /gene="ucpA"
                     /locus_tag="JQH51_000027"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY6074532.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22518..23519)
                     /gene="murR"
                     /locus_tag="JQH51_000028"
     CDS             complement(22518..23519)
                     /gene="murR"
                     /locus_tag="JQH51_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY6074533.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            23482..24378
                     /gene="murQ"
                     /locus_tag="JQH51_000029"
     CDS             23482..24378
                     /gene="murQ"
                     /locus_tag="JQH51_000029"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY6074534.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24382..25806
                     /gene="murP"
                     /locus_tag="JQH51_000030"
     CDS             24382..25806
                     /gene="murP"
                     /locus_tag="JQH51_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY6074535.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(25986..26885)
                     /gene="yfeX"
                     /locus_tag="JQH51_000031"
     CDS             complement(25986..26885)
                     /gene="yfeX"
                     /locus_tag="JQH51_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY6074536.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26981..27556)
                     /locus_tag="JQH51_000032"
     CDS             complement(26981..27556)
                     /locus_tag="JQH51_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY6074537.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27617..28066)
                     /locus_tag="JQH51_000033"
     CDS             complement(27617..28066)
                     /locus_tag="JQH51_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY6074538.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(28053..28478)
                     /locus_tag="JQH51_000034"
     CDS             complement(28053..28478)
                     /locus_tag="JQH51_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY6074539.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28692..29561
                     /gene="amiA"
                     /locus_tag="JQH51_000035"
     CDS             28692..29561
                     /gene="amiA"
                     /locus_tag="JQH51_000035"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY6074540.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29565..30464
                     /gene="hemF"
                     /locus_tag="JQH51_000036"
     CDS             29565..30464
                     /gene="hemF"
                     /locus_tag="JQH51_000036"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY6074541.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30470..31522)
                     /gene="eutR"
                     /locus_tag="JQH51_000037"
                     /pseudo
     CDS             complement(30470..31522)
                     /gene="eutR"
                     /locus_tag="JQH51_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(31568..32068)
                     /gene="eutK"
                     /locus_tag="JQH51_000038"
     CDS             complement(31568..32068)
                     /gene="eutK"
                     /locus_tag="JQH51_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY6074542.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32081..32740)
                     /gene="eutL"
                     /locus_tag="JQH51_000039"
     CDS             complement(32081..32740)
                     /gene="eutL"
                     /locus_tag="JQH51_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY6074543.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            33922..34713
                     /locus_tag="JQH51_000040"
     CDS             33922..34713
                     /locus_tag="JQH51_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074544.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            35433..35828
                     /locus_tag="JQH51_000041"
     CDS             35433..35828
                     /locus_tag="JQH51_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6074545.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            35846..36109
                     /locus_tag="JQH51_000042"
     CDS             35846..36109
                     /locus_tag="JQH51_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074546.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            36132..36674
                     /locus_tag="JQH51_000043"
     CDS             36132..36674
                     /locus_tag="JQH51_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074547.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            36771..37100
                     /locus_tag="JQH51_000044"
     CDS             36771..37100
                     /locus_tag="JQH51_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074548.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            37139..37345
                     /locus_tag="JQH51_000045"
     CDS             37139..37345
                     /locus_tag="JQH51_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074549.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            37804..38142
                     /locus_tag="JQH51_000046"
     CDS             37804..38142
                     /locus_tag="JQH51_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6074550.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            38123..38401
                     /locus_tag="JQH51_000047"
     CDS             38123..38401
                     /locus_tag="JQH51_000047"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074551.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            38394..39854
                     /locus_tag="JQH51_000048"
     CDS             38394..39854
                     /locus_tag="JQH51_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6074552.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            39876..40697
                     /gene="murI"
                     /locus_tag="JQH51_000049"
     CDS             39876..40697
                     /gene="murI"
                     /locus_tag="JQH51_000049"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY6074553.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(40816..41510)
                     /locus_tag="JQH51_000050"
     CDS             complement(join(40816..41264,41264..41510))
                     /locus_tag="JQH51_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY6074554.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(42029..43402)
                     /gene="eutA"
                     /locus_tag="JQH51_000051"
     CDS             complement(42029..43402)
                     /gene="eutA"
                     /locus_tag="JQH51_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY6074555.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(43377..43856)
                     /gene="eutP"
                     /locus_tag="JQH51_000052"
     CDS             complement(43377..43856)
                     /gene="eutP"
                     /locus_tag="JQH51_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY6074556.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(43869..44204)
                     /gene="eutS"
                     /locus_tag="JQH51_000053"
     CDS             complement(43869..44204)
                     /gene="eutS"
                     /locus_tag="JQH51_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY6074557.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(44497..46776)
                     /gene="maeB"
                     /locus_tag="JQH51_000054"
     CDS             complement(44497..46776)
                     /gene="maeB"
                     /locus_tag="JQH51_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY6074558.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47065..48015
                     /gene="tal"
                     /locus_tag="JQH51_000055"
     CDS             47065..48015
                     /gene="tal"
                     /locus_tag="JQH51_000055"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY6074559.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            48035..50038
                     /gene="tkt"
                     /locus_tag="JQH51_000056"
     CDS             48035..50038
                     /gene="tkt"
                     /locus_tag="JQH51_000056"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY6074560.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50141..51184)
                     /locus_tag="JQH51_000057"
     CDS             complement(50141..51184)
                     /locus_tag="JQH51_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY6074561.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(51310..51885)
                     /gene="nudK"
                     /locus_tag="JQH51_000058"
     CDS             complement(51310..51885)
                     /gene="nudK"
                     /locus_tag="JQH51_000058"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY6074562.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(51953..53932)
                     /gene="aegA"
                     /locus_tag="JQH51_000059"
     CDS             complement(51953..53932)
                     /gene="aegA"
                     /locus_tag="JQH51_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY6074563.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            54138..55855
                     /gene="narQ"
                     /locus_tag="JQH51_000060"
                     /pseudo
     CDS             54138..55855
                     /gene="narQ"
                     /locus_tag="JQH51_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            56057..59170
                     /gene="acrD"
                     /locus_tag="JQH51_000061"
     CDS             56057..59170
                     /gene="acrD"
                     /locus_tag="JQH51_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY6074564.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(59269..59328)
                     /gene="ypfM"
                     /locus_tag="JQH51_000062"
     CDS             complement(59269..59328)
                     /gene="ypfM"
                     /locus_tag="JQH51_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY6074565.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            59709..60065
                     /locus_tag="JQH51_000063"
     CDS             59709..60065
                     /locus_tag="JQH51_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY6074566.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            60069..61196
                     /gene="dapE"
                     /locus_tag="JQH51_000064"
     CDS             60069..61196
                     /gene="dapE"
                     /locus_tag="JQH51_000064"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY6074567.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            61224..61424
                     /locus_tag="JQH51_000065"
     CDS             61224..61424
                     /locus_tag="JQH51_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY6074568.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(61534..62232)
                     /gene="ypfH"
                     /locus_tag="JQH51_000066"
     CDS             complement(61534..62232)
                     /gene="ypfH"
                     /locus_tag="JQH51_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6074569.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(62306..64321)
                     /locus_tag="JQH51_000067"
     CDS             complement(62306..64321)
                     /locus_tag="JQH51_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY6074570.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(64336..65199)
                     /locus_tag="JQH51_000068"
     CDS             complement(64336..65199)
                     /locus_tag="JQH51_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY6074571.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(65367..66080)
                     /gene="purC"
                     /locus_tag="JQH51_000069"
     CDS             complement(65367..66080)
                     /gene="purC"
                     /locus_tag="JQH51_000069"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY6074572.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(66293..67327)
                     /gene="bamC"
                     /locus_tag="JQH51_000070"
     CDS             complement(66293..67327)
                     /gene="bamC"
                     /locus_tag="JQH51_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY6074573.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(67344..68222)
                     /gene="dapA"
                     /locus_tag="JQH51_000071"
     CDS             complement(67344..68222)
                     /gene="dapA"
                     /locus_tag="JQH51_000071"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY6074574.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            68368..68940
                     /locus_tag="JQH51_000072"
     CDS             68368..68940
                     /locus_tag="JQH51_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY6074575.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            68940..69410
                     /gene="bcp"
                     /locus_tag="JQH51_000073"
     CDS             68940..69410
                     /gene="bcp"
                     /locus_tag="JQH51_000073"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY6074576.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            69663..70280
                     /gene="hyfA"
                     /locus_tag="JQH51_000074"
     CDS             69663..70280
                     /gene="hyfA"
                     /locus_tag="JQH51_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY6074577.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            70280..72297
                     /gene="hyfB"
                     /locus_tag="JQH51_000075"
                     /pseudo
     CDS             70280..72297
                     /gene="hyfB"
                     /locus_tag="JQH51_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            72308..73255
                     /gene="hyfC"
                     /locus_tag="JQH51_000076"
     CDS             72308..73255
                     /gene="hyfC"
                     /locus_tag="JQH51_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY6074578.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            73272..74711
                     /locus_tag="JQH51_000077"
     CDS             73272..74711
                     /locus_tag="JQH51_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY6074579.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            74723..75373
                     /gene="hyfE"
                     /locus_tag="JQH51_000078"
     CDS             74723..75373
                     /gene="hyfE"
                     /locus_tag="JQH51_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY6074580.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            75378..76964
                     /gene="hyfF"
                     /locus_tag="JQH51_000079"
     CDS             75378..76964
                     /gene="hyfF"
                     /locus_tag="JQH51_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY6074581.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            76954..78668
                     /gene="hyfG"
                     /locus_tag="JQH51_000080"
                     /pseudo
     CDS             76954..78668
                     /gene="hyfG"
                     /locus_tag="JQH51_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            78678..79223
                     /gene="hyfH"
                     /locus_tag="JQH51_000081"
     CDS             78678..79223
                     /gene="hyfH"
                     /locus_tag="JQH51_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY6074582.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            79220..79978
                     /gene="hyfI"
                     /locus_tag="JQH51_000082"
     CDS             79220..79978
                     /gene="hyfI"
                     /locus_tag="JQH51_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY6074583.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            79971..80384
                     /gene="hyfJ"
                     /locus_tag="JQH51_000083"
     CDS             79971..80384
                     /gene="hyfJ"
                     /locus_tag="JQH51_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY6074584.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            80414..82243
                     /locus_tag="JQH51_000084"
     CDS             80414..82243
                     /locus_tag="JQH51_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY6074585.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            82244..82426
                     /locus_tag="JQH51_000085"
     CDS             82244..82426
                     /locus_tag="JQH51_000085"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HAY6074586.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            82448..83320
                     /locus_tag="JQH51_000086"
                     /pseudo
     CDS             82448..83320
                     /locus_tag="JQH51_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(83414..84475)
                     /locus_tag="JQH51_000087"
     CDS             complement(83414..84475)
                     /locus_tag="JQH51_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY6074587.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            84688..86151
                     /gene="bepA"
                     /locus_tag="JQH51_000088"
     CDS             84688..86151
                     /gene="bepA"
                     /locus_tag="JQH51_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY6074588.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            86172..86531
                     /gene="arsC"
                     /locus_tag="JQH51_000089"
     CDS             86172..86531
                     /gene="arsC"
                     /locus_tag="JQH51_000089"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY6074589.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(86664..87410)
                     /gene="hda"
                     /locus_tag="JQH51_000090"
     CDS             complement(86664..87410)
                     /gene="hda"
                     /locus_tag="JQH51_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY6074590.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(87460..88749)
                     /gene="uraA"
                     /locus_tag="JQH51_000091"
     CDS             complement(87460..88749)
                     /gene="uraA"
                     /locus_tag="JQH51_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY6074591.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(88835..89461)
                     /gene="upp"
                     /locus_tag="JQH51_000092"
     CDS             complement(88835..89461)
                     /gene="upp"
                     /locus_tag="JQH51_000092"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY6074592.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            89785..90822
                     /gene="purM"
                     /locus_tag="JQH51_000093"
     CDS             89785..90822
                     /gene="purM"
                     /locus_tag="JQH51_000093"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY6074593.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            90822..91460
                     /gene="purN"
                     /locus_tag="JQH51_000094"
     CDS             90822..91460
                     /gene="purN"
                     /locus_tag="JQH51_000094"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY6074594.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            91632..93698
                     /gene="ppk1"
                     /locus_tag="JQH51_000095"
     CDS             91632..93698
                     /gene="ppk1"
                     /locus_tag="JQH51_000095"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY6074595.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            93703..95244
                     /gene="ppx"
                     /locus_tag="JQH51_000096"
     CDS             93703..95244
                     /gene="ppx"
                     /locus_tag="JQH51_000096"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY6074596.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(95283..>96391)
                     /gene="pdeF"
                     /locus_tag="JQH51_000097"
     CDS             complement(95283..>96391)
                     /gene="pdeF"
                     /locus_tag="JQH51_000097"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY6074597.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        23374 a        25142 c        24273 g        23602 t
ORIGIN      
        1 cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg agctatagcc ccgtcacgta
       61 aagcttgtcg agttgacggg cggcatcata tgaattccgc ccgaatgtgt caacggcaaa
      121 ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc
      181 tcgcattcag tagttattca tgtcacggtt tcctgtaaag tggtgttata aaatgaacta
      241 ctaatagacc cacatacatt cagggaattg ttatgttcaa ggagaggatg acaccagatg
      301 aacttgccag actgaccggc tatagccgcc agaccattaa taaatgggta cgcaaggaag
      361 gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa agcccgactg gttcacgtca
      421 atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc
      481 ctgccctttc tggtgatgcg ccgcttgaag tattgctggt gacactggcg aaagagatga
      541 cgccagttga acaaaaacag tttacatcct tgcttctgcg ggaagggatt atcggattgt
      601 tacaacgctt agggattcgc gatagcaaat aatatgaaaa gattacgcaa taaaatgacc
      661 accgaagaac tggctgaatg cctcggtgtg gctaaacaaa ctgttaaccg ttggaccaga
      721 gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt
      781 ctggtcgata cgcaagtttg cgagtttatt cagaacacgc cagccttcca taacacacca
      841 atgttgatgg aagctgaaga gagaattgct gaatacgctc ctggagcacg cgccccggcg
      901 tatcgacaaa tcatcaacgc gatagataat atgacagaca ttgagcagga aaaagtcgcg
      961 caatttttgt cacgtgaagg aattcgcaat ttcctcgccc gtctcgatat tgacgaatca
     1021 gcataaataa aaaacggcag gatattatct cctgccgttt atctttttac acgctaatta
     1081 ctgctgattt tcgcgttcag caataaaatc cagcgctttg ttgatacgct cgatactgcg
     1141 ggtcttacca attgcgtgaa cggtaacatc cagtgctgga gactgccccg caccggttac
     1201 ggcgacacgc agcggcatac caactttacc catacccact tccagctcat ccgccgtcgc
     1261 ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta atcgcggcca gtttgtcacg
     1321 aaccacttcc agcggctgac gcgctaccgg acgcagatgt tttttcgccg catcggcatc
     1381 gaactcagca aaatcttcgt agaaataacg gcagctctgt gccatctctt tcagcgtctt
     1441 gcagcgttcg cccagcagtt tcaccagatc agccagctgc gggccgttac gggtatcgat
     1501 attttcctgc tcaatgtgcc actgtaagtg agtagcaaca tactccggcg gcagcgcgtt
     1561 aatgtagtga tggttcagcc acagcagctt gtcggtgttg aacgcactgg cagatttgct
     1621 gacggcattc aaagtgaagt atttgatcat ctcttcacga gtgaagattt cctgatcgcc
     1681 gtgggaccag cccagacgca ccagatagtt cagcagtgct tctggcaaat aaccgtcatc
     1741 acgatactgc attacgctga ctgccccgtg acgtttggac agttttttac cgtcatcgcc
     1801 attgatcata gaaacgtgcg cgtaaaccgg caccggcgct ttcagggcct taagaatgtt
     1861 gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg ataacgtggg tgatttccat
     1921 atcccagtca tcgacaacca cacagaagtt ataggttggg gaaccatcgg tacggcggat
     1981 aataagatcg tccagttcct ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa
     2041 aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca cacggttcat catcagcatg
     2101 atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc ttctcacctt tcgccatttg
     2161 ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag catttataag cagtgccctc
     2221 ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg
     2281 accttcatcc cactccaggc tcagccagtt catgccatcc ataatggctt cgatagcttc
     2341 cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc acgaactcac cgccgtggtt
     2401 acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt
     2461 tgggcttggc gcgaagcgag ttttgatttt catgaaatgg ccttacgttt agaaagatgc
     2521 cgacaaccgg caaatcctgg aaaaattgag tgggcgatat tctatcactc ccgcctgatt
     2581 cctcaatgta gttcgggttt ctaccttacg cattattgtt ttcgttgaca aattgcgcaa
     2641 cacggtctgt tttgcgatca tcttgcttaa atttacgacg aacgaacaat ttctttagaa
     2701 aatgcgttga ctcattttga actctcccta taatgcgact ccacacagcg ggggtgatta
     2761 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
     2821 cccaccaact actttatgta gtctccgccg tgtagcaaga aattgagaag tgggtgatta
     2881 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
     2941 cccaccactt cgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg
     3001 caggttcgaa tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc
     3061 tatctgcgat actcaaagat tcgaacctgc agcaggtttg agttgagcgc agcgaaacaa
     3121 cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc gagtcatcct gcacgaccca
     3181 ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt aagattgatt
     3241 tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac gaggactgaa
     3301 caatggccac tatctcttca acttcaattc cttccataca aacacagagt agtaacaggg
     3361 catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa atcatcaaac
     3421 tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac aagcaaaaac
     3481 aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg cagttgcaga
     3541 aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctgaat gtatcccttc
     3601 taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac ggatcccctt
     3661 cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac gcttcagcgg
     3721 cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca accaccggcg
     3781 taatgcgctt aaccaccagt gggctacctt caggcaatgg tagcgccagt gcaggaagaa
     3841 tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt ccaatctcct
     3901 gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc ggtcggctgc
     3961 ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag ggtacataat
     4021 cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg gaaagaatcg
     4081 cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag tcgacatctc
     4141 cctggcgaat actttccatc acccactgct gcggacgatc atgcaggaca aactgaatat
     4201 ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg gaaatggtct
     4261 ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg gtatcgcgca
     4321 acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc tgccctgcat
     4381 ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta tgattttcca
     4441 gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct cctgcacgac
     4501 taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa gaataattca
     4561 tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact ggaagaagca
     4621 caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct cgatcctttc
     4681 accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag aggcgatttc
     4741 gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt tatgcacggc
     4801 gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact gcatttgtgg
     4861 gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc ctggtggaaa
     4921 ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcat tctcccggct
     4981 accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc ggcggcggtt
     5041 tgttctgcgt cagcatccag cctgctgggg attttccttt caccattgct ggttggtctg
     5101 gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg tcggtaaaat tatgctgcaa
     5161 ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga ctgggtgtcg
     5221 cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt ggtttataca
     5281 gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg atcattgctg
     5341 tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa cgtctttatg
     5401 gcacgccgac tgagcttcaa taaggcagat gaaattacta tcgtcttttg tggttcgaaa
     5461 aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc ggtgatcggt
     5521 atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg tgcggtgctg
     5581 gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag cagcgccgat
     5641 aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc ggttttaatc
     5701 agcagcgtga tttgcattag ctcaccgagc ttccctgtcg ggaactcatc tttgcgggca
     5761 aaccacagca gatactcctc cggcaggtca attaagcgac gccctttgta tttgccaaac
     5821 ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc tacccagcaa
     5881 acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct tcgccagttt
     5941 agatcctgca gcttcacccg ctatcaccag atcggttttc ttcgacacgc tgcccgcgac
     6001 tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct ggcttaagct
     6061 gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg cgttgataac
     6121 gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat tgcggttgct
     6181 ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat caggcacctt
     6241 ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat atgccgccag
     6301 acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga agcgagcaaa
     6361 ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccaatt ttggccccat
     6421 acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg gagtatggac
     6481 atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa catccatcgc
     6541 acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa tcaggccacc
     6601 ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac ataccggaca
     6661 atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca cgacgttaac
     6721 cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa tgcgtaaacc
     6781 aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca cgccagcaac
     6841 atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa cctgaaactc
     6901 gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcaa ccgcccagcg
     6961 cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga ccttaatcac
     7021 cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt gatagaacgc
     7081 cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca ccccccactt
     7141 tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt ccagaacacc
     7201 aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat caagctgacg
     7261 cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg tgcgtcgcgc
     7321 atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac gcacttccag
     7381 acgcgccggg atattctctc cgtgcagctt cagcggaatg gcgcgaatag tacgcacatt
     7441 agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga ctaaaacgcc
     7501 attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc accaggtgac
     7561 tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa agctttcttc
     7621 atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc tgaaagctgc
     7681 cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt ctggatgttt
     7741 ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg gaatttccgg
     7801 cgcatccatc acatgataaa gatattcatg atggcaaagc gtcgttcgca gttctgtcag
     7861 ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga caagcggggg
     7921 ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg acttcgcgga
     7981 tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga tcgtcaagca
     8041 cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc ttgaagttct
     8101 gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga gtagtgaaat
     8161 ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg aataacgccg
     8221 ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca aaaatgaagc
     8281 ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg tgatgcgccg
     8341 cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact ggagccggtt
     8401 ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa gcctgttgtg
     8461 ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc gaaactggct
     8521 gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg ctgcaccggc tgcgctggac
     8581 gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt tgacgcggct
     8641 gcgcagacgc ataaggcggt aggtactggt gttgcggcga cggacgagca gcctcatgct
     8701 cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
     8761 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
     8821 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca tgtaccagta
     8881 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
     8941 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
     9001 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
     9061 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
     9121 atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
     9181 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
     9241 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
     9301 gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactttc atgagttacg
     9361 ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc
     9421 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
     9481 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
     9541 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgtct
     9601 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
     9661 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
     9721 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
     9781 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
     9841 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
     9901 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
     9961 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    10021 tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    10081 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    10141 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    10201 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    10261 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc
    10321 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    10381 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    10441 aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    10501 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    10561 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    10621 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    10681 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    10741 gttatcgccc aggtgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    10801 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    10861 atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt
    10921 gcaggtatct cttctggagc agctgttgcc gcagcgttga aactacaaga agatgaaagc
    10981 tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc
    11041 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    11101 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    11161 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    11221 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    11281 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    11341 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    11401 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    11461 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    11521 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    11581 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    11641 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    11701 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    11761 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    11821 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttga
    11881 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    11941 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    12001 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    12061 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    12121 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    12181 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    12241 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    12301 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    12361 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    12421 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    12481 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    12541 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    12601 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    12661 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    12721 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    12781 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    12841 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    12901 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    12961 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    13021 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    13081 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    13141 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    13201 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    13261 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    13321 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    13381 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    13441 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    13501 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    13561 aatctctggt ttccgacgac aaaaaggata ccggaactat tgagatcatt gctccgctct
    13621 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    13681 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca
    13741 ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac
    13801 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    13861 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    13921 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    13981 aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca
    14041 agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg
    14101 ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca
    14161 gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc cttccctttc agcaaaccac
    14221 tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat
    14281 gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt
    14341 cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg
    14401 cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa
    14461 agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa
    14521 ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca
    14581 ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg
    14641 atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct
    14701 gaagcgcacg caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt
    14761 cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct
    14821 gttttatagc aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca
    14881 cggcgacgat atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca
    14941 tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt
    15001 ctggcagtca aatcggctat tacaaaacaa agataaggta attcagtgaa gaaaatcatt
    15061 tgtctggcca ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa
    15121 gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg
    15181 cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc
    15241 aatacccgta accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca
    15301 tcgattaccg ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag
    15361 ccttttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa
    15421 tccgctttgg taaggcattt acaggaggta acatgaaaaa atgctttatt tatcacgatg
    15481 aaaaatcgaa taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg
    15541 gcaaggtagg tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc
    15601 tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag
    15661 actttaactt catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa
    15721 cgtcacaccc aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg
    15781 aagaatctcc ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc
    15841 tccgttctgg actgtgctga tttccctcaa atgttaattg aaactatgtg gggtatgaaa
    15901 tacatcgcta tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa
    15961 atgagctcta tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc
    16021 atgggtaaaa tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag
    16081 cttaccgcaa aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg
    16141 attgatgacc ttactgcttt tccacacgaa aattaaaaac tgcatttgtc ggcagcaaca
    16201 actgccgaca aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa
    16261 cgcctgatcc ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg
    16321 ataagcggag cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct
    16381 taaatccctg ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga
    16441 tcgccacgat cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc
    16501 agtgctccag caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc
    16561 agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc
    16621 gcagcgttga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg
    16681 ctgccttctt ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag
    16741 catgaaacgc cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc
    16801 ccgccggttt gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg
    16861 ggattattga actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga
    16921 tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca
    16981 cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag
    17041 cctttcagcg cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt
    17101 aagacatcac ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc
    17161 gcacgatctt tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg
    17221 ccgttatccg gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct
    17281 aatgtactca caatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta
    17341 tcaggcctac aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg
    17401 ggcgtttaac attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg
    17461 ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg
    17521 ctcgccatgc tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca
    17581 ccccagcggc tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg
    17641 taccggcagc ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag
    17701 gaagagatcc accggcccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc
    17761 aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc
    17821 gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat
    17881 attcccctga ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg
    17941 ggtcacaaac acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag
    18001 ctctttacgc acctgcgcat ccagcgcgcc gaacggttca tcaagcagca gaatttgcgg
    18061 ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc
    18121 cggataacga tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc
    18181 tttgatggct gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat
    18241 attgtcgaat accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt
    18301 acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt
    18361 ttgatgctcc agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc
    18421 cagcaacgcg accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt
    18481 gcgaccaaaa gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg
    18541 cgcgtttttc ctgattctcc aggcgccact gcaacatact ctttaaaaac agggtgataa
    18601 tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct
    18661 ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca
    18721 ccgacactgc accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca
    18781 gcgcccagcg aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa
    18841 gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc
    18901 gcaccacaaa cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca
    18961 taatttgcag attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca
    19021 gcagatacac cagaccggca accaccggcg atacagcaaa cggaatgtcc agtagcgtca
    19081 gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga
    19141 acaccaggtt taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca
    19201 gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca
    19261 cgaagatgta aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa
    19321 tcagaaacca tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt
    19381 ccgccattaa tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa
    19441 cagcagcagc agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc
    19501 aaactcctgt aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt
    19561 tccggcgata aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac
    19621 gcccgccacc agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg
    19681 cgttgcacca agcgtttccg ccgcttcttc atattccggg cctaactctt ccagcaccgg
    19741 ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag
    19801 ccaggtatag gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac
    19861 ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc
    19921 catcagcgca tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat
    19981 cagcagaccg aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt
    20041 gtaggccgcg accacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc
    20101 agaaagctgc atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag
    20161 actggtgcca aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat
    20221 tacttacgcc ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc
    20281 atcacttccg gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat
    20341 ttatctttca gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg
    20401 atggtctgcg cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta
    20461 ccgttggcct gcacattttt atcgacccac gccaccggga attccgccag aatattggtt
    20521 ttcgggatca ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc
    20581 gattcaaagc taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca
    20641 cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt
    20701 ttggctttat cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa
    20761 cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg
    20821 tcgttccagt cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag
    20881 tagaacggcg agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct
    20941 ttgtcgtgca ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag
    21001 ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt
    21061 ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac
    21121 agctcgcggg agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc
    21181 agcagcagag aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg
    21241 cacccttata aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga
    21301 gtaacggttt tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt
    21361 caacaggcgt aattgttgca ggcagtttgg acacggacag cgcggagaaa ccggagcgta
    21421 cacatagtac gtgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct
    21481 ggtgaactta gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat
    21541 cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta
    21601 ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc
    21661 ggatcggcga ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga
    21721 tcttccgggt tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg
    21781 cagatggcgt taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg
    21841 ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca
    21901 ccagtgactg aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc
    21961 accgccttcg tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct
    22021 tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt caccaggata
    22081 tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca
    22141 cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc
    22201 ttttcgatct caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt
    22261 ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg
    22321 agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga
    22381 aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat
    22441 ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca
    22501 ttttcaatga cgccacatca ttgatgttgc acaagaccaa caaacagtaa atcggtaaca
    22561 gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg
    22621 gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca
    22681 atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg
    22741 ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct
    22801 tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac
    22861 agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta
    22921 ctgatgacct caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc
    22981 agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga atcatcactg
    23041 gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat
    23101 tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt ctgggcaaat
    23161 ttcacaatgc tcgactggct gataccgagc tgtttcgcca tctgccgtga agagacggat
    23221 ttcagctcgc tcactctggc ccgcagaaag tcagcaattt tttgctcgtt cccggtaaat
    23281 tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca
    23341 gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg
    23401 attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg
    23461 taaatagtaa ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc
    23521 gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa
    23581 gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg atatcgccgc ggcgatcgat
    23641 gttatccacg cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc
    23701 ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt
    23761 ctggtggttg gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggagct
    23821 gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat
    23881 gtggtggttg gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac
    23941 gcacgccagc tcggctgccg cacagtggga atttcctgta atccggggag cgccgtttca
    24001 accaccgctg agtttgccat tacaccgatt gtaggtgccg aagttgttac cggttcttcg
    24061 cggatgaaag caggtacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg
    24121 attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa
    24181 ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg
    24241 gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa
    24301 aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag
    24361 gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt
    24421 cttacccgtg tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg
    24481 cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg
    24541 aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat
    24601 cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac cggtacagga tgccgcagaa
    24661 atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt
    24721 ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg
    24781 ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc
    24841 acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttccta
    24901 gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca
    24961 attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc
    25021 ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg
    25081 attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat
    25141 ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg
    25201 atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg
    25261 aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt
    25321 ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac
    25381 agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc
    25441 tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc gattattccc
    25501 ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg catgaagccg
    25561 tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg
    25621 tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg
    25681 ctgatgactt ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg
    25741 gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg
    25801 gactgatatt atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt
    25861 cagcgccgca aaataccctg ttctgacaga aggtagttga tcaagtatat gcggcgttaa
    25921 agcagtaata aaaaggccag tcagtaatgt tactgcctgg cctttaatca atgaatcaga
    25981 aacgcttaca gcgccatcaa cttgtccagc gacggtgcga aataatagcc gccggttacc
    26041 ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg tatcgccaaa catgctcagc
    26101 agttgctgct caatgttatg cagacgcgcg cagtaggcgc agaagtacag accgtgagtg
    26161 ccactggcgg tgccgtacgg caggctctgg cgaacaatct tcagcccttt gccatcttct
    26221 ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt cgtcgccgtc aatctcttcg
    26281 ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt gaacgctcat ccggttgagc
    26341 tgcttcaggt tgtgctccca acgctgcaca aacacatagc tgccgccgga atccacgccg
    26401 tctttgataa ccgccacttc gcggcgcgtc tcttcacccg ccgggttttc tgtaccgtca
    26461 acaaagccgc tcaggtcacg ctcttccacc caacggaagc cgtggatctc ttctttcact
    26521 tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga cagagaagtt tacgtcgtga
    26581 cgcagagaaa gaatgtggat caacacatcg aactgggtcg tcggcgcaag gcctttaccg
    26641 tagcccggaa aatctttcag ctcttctgcc ccaacgccgc cgctcagagc gcgccaggtg
    26701 ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc ttcaaaagtt
    26761 gccagtttgt cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc ccctttcacg
    26821 ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca aaatgccact ctgaacctga
    26881 gacattgttc ctcctgaaaa taagaatgcc acgcaatccg tggcattgtt gagcgctatt
    26941 ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa ttaacgccgc cagataattt
    27001 tactgacttt ccagtttttc agggtatcgt ccgaaggcat taacccttca ggaccacgcc
    27061 attccccgct gaactggtag ctaatatgtt gactgccttc ggctttacat tcgacggcac
    27121 gattatcatc accatcagct ttttggcaat tgccaaatgc tttgctgtaa aggtcgctaa
    27181 acggtgtacc gattttaaca ccggtgtcag caggaatatc gctatccagc acgtcaatgc
    27241 gactgatcgt gccttgatcg ccgttaatca ccatcgccac gttgtcgcct ttcatcactt
    27301 caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc gctgcgcagg cgataatcgc
    27361 catcaagcgc atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt aattcaccca
    27421 cgccctgctc gctcactttg gtggacgatc caaaccagtt ccacgggtta gcggcagacc
    27481 agttaactga cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg cataacatta
    27541 aacgcagcga tttcatgtca cttcctttgg ttattcaata acgttgcttg gagtgcaaat
    27601 tcaccaaaaa gtgccgttat tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg
    27661 tcagcagcca gattaacgcc acaatatccg ccactaccag cgccagaccg ataccgctaa
    27721 cggattcacc gttcagccac agccacggtt gccagcaaag taaaaccacc tgcgccaaca
    27781 gcaacagaaa atagagcaca tgccaggtgc gaggaaacga agcccgccga ccgctcaaca
    27841 aaaacgccag caccgccgga ataccgggaa tcagccccag ccagaaatta tcgtgatcgg
    27901 gataaaacag atttagcagc gcagtaccct gctcacgcga cgcaccggca atgacaaaca
    27961 gcacccaggt tcgcgcctga agcaatagca caagccagaa gagcaagggt aaacgcaggc
    28021 gtccgtgcgc atcataatgg acaggatgga actcagtact cttcatcttc aatcaaacgc
    28081 ttacccagac tcagcacgtc ggcgtgttca tatcccaggc gttcatacat tccgagcacc
    28141 atgtcgttat cttccggcac gttgatctga attttcgggc agccgcgagc aatcagcttt
    28201 ttctccagcc ggttaagcaa cgcattggca attccacgcc cacgaaactc tggatgcacg
    28261 ccaagataat aagcagaccc gcgatgcccg tcataaccgc ccatcaccgt tccgaccact
    28321 tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg
    28381 tccatttccg gatcgttcca cggacgcagc aagtcgcaac gctcccaaag ggtgatgacc
    28441 tcttcgaaat cttcctggcg aaatacgcgt atctccatgg tattcgttac ctttttgcgg
    28501 gttaaaaggc tgattatggc gtgaacggtc gaattagcca atatctgacg aaaatcggtt
    28561 gaaaaagtgg cataatgggg agttgtcaac tattgaaatg aaaagtaaaa caattctcaa
    28621 cagcaaaccg tcgtaacgga ttacgcgata cgatataaca tctggaactt tattattaca
    28681 actcaggccg tatgagcact tttaaaccac taaaaacact cacttcgcgc cgccaggtgc
    28741 tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc gcaagccatt gccaaagacg
    28801 aacctttaaa aaccagcaac ggacacagca agccgaaagc caaaaaatct ggcggcaaac
    28861 gtgtcgttgt tctcgatcca ggccacggcg gaattgatac cggagcgatc ggacgcaacg
    28921 gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta
    28981 atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca ctttacgatc
    29041 gcgttgaaat cgcccataaa catggcgcag atctgtttat gtcaattcat gccgatggct
    29101 ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta
    29161 gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa
    29221 aggcgactga caaggatcac ctattgcagc aagtactgtt tgatctggtg caaaccgata
    29281 ctattaaaaa cagcctgacg cttggctcgc atattctgaa aaagattaag ccggtgcata
    29341 aactgcacag ccgcaacacc gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc
    29401 cttcggtgct ggtggaaacc tcgtttatca ccaacccgga agaagagcgg ctgttaggca
    29461 cggcggcgtt tcgtcagaaa atcgccacag cgattgctga aggcgtgatc agttatttcc
    29521 actggttcga caaccagaaa gcacattcga aaaagcgata agttatgaaa cccgacgcac
    29581 accaggttaa acagtttctg ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg
    29641 tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga agctggcggc ggcgggcgca
    29701 gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg cgtcaacttt tctcacgtcc
    29761 acggtgaggc gatgcctgct tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt
    29821 tcgaggcgat gggcgtttca ctggtagtgc atccgcataa cccgtatgtt cccaccagcc
    29881 acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc agagcccgtt tggtggtttg
    29941 gcggcggctt cgatttaacc cctttctatg gttttgaaga agacgccatt cactggcacc
    30001 gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt ttatccccgt tacaaaaagt
    30061 ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca gcgcggcatt ggcgggctgt
    30121 tctttgatga tctgaacacg ccagatttcg accactgttt tgcctttatg caggcggtag
    30181 gcaaaggcta caccgacgct tatttaccaa ttgtagagcg acgtaaagcg atggcctacg
    30241 gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg
    30301 tctgggatcg cggcacgctg tttggcctgc aaactggcgg gcgcaccgag tcgatcctga
    30361 tgtcaatgcc gccactggta cgctgggagt atgattatca gccaaaagat ggcagcccag
    30421 aagcggcgtt aagtgagttt attaaggtca gggattgggt gtaactccct cacccccatt
    30481 cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc aaacagctgc tggtaatccg
    30541 tggcaaattg ccccagatgc cagaatcccc actgcatggc ggcgtctttt accgttgtgc
    30601 tttgcgacca cgggcttatc agttcgcggc gtacgacgtt caggcgaatg cgtttcagcc
    30661 acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga
    30721 catgcagttg attacacaaa tccagcaccg tcaccggttc ggacatgttt tccagcacat
    30781 attcacgggc gcgggaaagc aaccgacggt aactctgatg actgatgctt tccgccgtca
    30841 ccattggttg cgcttcttcc agcatcgccc ccatcgccat tagcaaatta tcccccagca
    30901 cttttcgcac tgcaggctga tggagatttt ccgggttctc gcaaaacgtc gccagcgcct
    30961 gttggacaaa gccccacagc gcggctttat gctgctcttt cacttccagc gccgactggc
    31021 tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa gttagcctga caggtgatga
    31081 catcttcaga cagcactacg cccaggatcg tataatcgtc cggcgtactt aattcaaact
    31141 cggttccacc ggggcgcgtc gctatttctg cgcttcccag acattgcgaa ccgataaatc
    31201 cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga gttcggccag accaggcacg
    31261 actgacgcag cgccagaccg gtgtattcac gaaaaacctg aatatcatcg agtagaattt
    31321 ccgtaaattc accatgaaac ttgcccggat gcagctgatc gtaaatctgc tgctaggcgg
    31381 taatcgtcaa agcatgttca tagacatccg ttgtctgtcg ttgatgaaca ttatccactt
    31441 cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc atgataaaga tggtgcaaat
    31501 tggctgtacg ggtctttttc atgatgttaa tgccgggtgt tgtaggacac ccggcacctc
    31561 cgacaggtta atggggcttg agacgataac gactactgcg tttacgtaac gtcccggcag
    31621 aaaagagctg ttcgagcgca tttctggctt tttccagcgg ccagccaaag tgggcggcca
    31681 cttctcctgc cgtcattccc tgacatactg atgttaacag cgccaacaac tcgtcagcag
    31741 attccgcgac gataactggc gcgtcgggtt cctttacggg ttgcttcggc tggcggttaa
    31801 agccagtaac cagccactgg gtgtcatcgt ctggccgacc gatctcctta cggctgatga
    31861 cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag cgccgcacga cacgccgcca
    31921 gatcgccttc caccaccagc gttaagcgac cagggtcaag cacttcgtga ctgagcagac
    31981 gaacgttagc tgctttgagc atggcatccg ctgcatctat cgcggcgacc atgccgtcca
    32041 cttccagcaa tcccagtgca ttgatcatcg gcaacctccg ttacgcacgc tggattggat
    32101 tacgcgcgat ttccagcact gcatcggtaa aggcgttaca ggctgcttta cacgcggcct
    32161 ggctaccggt taaaaatgct gccgagtagt tggtttcaga cggtggcggg acgtaggtca
    32221 ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc gtaggtcgct tccagcggcg
    32281 gtgccaccag atacgccatc ggatcgccaa gcgtgatccc ggcggttgat gaaagataag
    32341 aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt ttctgcgtca ttggcccact
    32401 ggaaagctgc gccattttca atatgtgcaa ccatcgcatc cagaccggca cgcacttccg
    32461 ccgggtttgg cccaccaagc ataatcagca cttcaccggc agtcggtgac gggccgtgtg
    32521 ccgctcccgc atacagcgag cgaccatata ccacttcgac catcgcttgt ttggtcgctt
    32581 cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga aatcagcccg aggctacgaa
    32641 tatgcggcgg caattttagt tcacgcgcaa attcagcgtt aacggaggca atcacccgca
    32701 tggcggttac cgacggtcga atcaaatcta aagctggcat gatgcctcct tatcgggtca
    32761 tgttgatgcc ggatgctttc tgctccagca tacgtttcgc caaatccaca atgacggcac
    32821 agcagcacga aagagaagca aaagtcagct acctggtaca ctaggcagcg ggtcagcaaa
    32881 ggggggcaaa tgcccctttt ttcatgtcag ataatcagat cattacctca ataaactaat
    32941 aatcacatcg cggttacaac cagtttgata gtgatagctc aaacccagac gtggcgctgg
    33001 ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt actagccgca aaaatgcggt
    33061 cctatttgcc agaacctttt attattcaat tggttagtgc aagaaaaagc gtgattactg
    33121 ttcaagaagt atccagaaag gtcatgattt tgacgctgaa aaaaaatagg tgggcatttt
    33181 tgcccactca ctagccgcaa atttgcccac tcactagccg catttttgcg gcccaaagta
    33241 ataaatacag agaaataata aatacagatc agcgccaaga tttttcgtct tttcggcgga
    33301 atcatgagac tagtgcttac gtcctgataa cctccagagc agaccttaac ttctgtgcag
    33361 caggactacg ctcattgccg ctcggctagg tcaaatcctc acgataaagc tgttcggatt
    33421 gtgaccataa tgttaaagga ctctttaaat cattccttga cgaattaact agctggctta
    33481 agcggaatag catgcaggat aaaaacaaaa ccagcttagc agtggggaga gcttcgcttc
    33541 gctcgtctcc tggggccaat gctcaatgaa acgacgtaag gcacggagcg ctcaccatag
    33601 gtagacacat aaacatctga gagcgcacct tctatcgcgc tacgcgctca ataaaaacca
    33661 acgacaccgc cacactgcaa gaccaatggt ttttatcgcg tatacagacc agacaggaaa
    33721 cagcagcaaa gcagcgtata gccaggtgca gacagcacac tcacacgacg cacagcaggc
    33781 ttaagtgatc actcaggaaa ctttatcccc gccgccgatc accgcgaact atcatgaccg
    33841 cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac acacataagc gctacactta
    33901 cttaacgtcg ccacagagcg attgagagcg cttaaaatcg atgcgatttc attaaatttt
    33961 tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa cattgccccg cctcgatatt
    34021 gagcgcataa tagatacata cattaagcgg aaaagagata agtttattgt gaaaatcagg
    34081 gggttacata tcatgcgcat atcagaacaa caacggcaga tcctcgacta tctggcaaaa
    34141 aacgaatcaa tcaccgcacg acaagccgca gatctcgttt atggtggcaa tgttacccgc
    34201 gtccaggttg acgccgcccg ccgctcactc atgacgatgg ttagtaatgg gctaatcagg
    34261 aaacaaggcc gcgctttcgt agctgaaaga catatcaatc acgcttatag ccaggacgtt
    34321 gaagaattgc gccgccagat cctttgggta ttcagcaaac agggtcagga gtatttcctt
    34381 tctgaacacc caaatttcgc cgccaaagag ttaacagtaa gcggattgtc gtgcgcactg
    34441 gattatcttg tcggtgaagg ttgcgttact tacaagcagc tatggcacgc cctgaatacg
    34501 cttgtagaag atggtttact cattgccacg cgtgagaaca tcgaatactg gataatgccg
    34561 cgttcaggtc gccgcaatca tgcacggtat atttcaggca acagatacag aacgccggac
    34621 tttaatccga cacaggaaga tcgggaccgt gccgaaatga tgtgcaggga acaggagaaa
    34681 agaattatgg ataagttatt ttcgatgcag tgaacacaag cgggcgcaat gcccgttttt
    34741 attgccttat tccacgcctg acgaatcaat ttgatcacac ctgagaaaat gcatagccag
    34801 aagttaaggc cgttatattg cgattgggag cgttttaaac ggtatcaatt taattaattc
    34861 tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa gcgggaaata gagacatatc
    34921 gttgtagtta ttaacagatc actttgaggt cgagggcggc taaccctcgc taatattatc
    34981 ccgctattac cttgcgggtg caaataatct tttattgctt tgatgattta ataaatagca
    35041 ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa atgtcaacag aaaaaataag
    35101 gtttataata ttaagtatca catgggtaat gggttcaggg aatcggaatc caccgcctca
    35161 cggaaattca aaatttgctt cttctttcct ccgtgggcgg cttctttttc gtatcgaggt
    35221 atttatgaaa gcaataacat tagaacagtt cgcagcaatg gcgaatgtat cgaccagaac
    35281 gctgaggcgc tggctaaaaa aaccatcaac cggagtccct ttgccattta ccccacgcgg
    35341 tactcgccaa ttattctggg agcacgaatg caaagaatgg ttgtacagaa cagaacgata
    35401 ataatcacgc atcacatttt gaggtaaaca tcatgaccaa tatcgaaaga ttagaacgct
    35461 gtgccgatat tatgcgacgc agatggattt atgaccctga agcgggttta ttgtttagcc
    35521 gtgaaacaaa agacgttatc aaaggcagca tgaccaataa agggcatttg gttgtaacgg
    35581 tacatgaagg taaattcaaa gtgacactca cctatcagaa agcctgctat gtatacacat
    35641 acggcgctta tgacgagaca ttatatgaag tgcaccacgt taatttgaac aaacaggata
    35701 accgcatcaa gaatattcgc ctgatgccga aagaaaagca ccgccggata cataaaaacg
    35761 tccgcaaact tattcggctg ggccacacac aatggcttca accgcaatta attgaacaat
    35821 cactataaac acaggagtaa ataccatgca ttcattagac agttattttc agcgcacgac
    35881 agcccctaaa tcagccgccc aggaacgccg tgaggaattc catgagaaag tgatgcgttc
    35941 tgctgattat attgcggaca agttcgtgga aacggttcgt ccacttgttg atgaagtagc
    36001 tgataaactt cagtctgaaa tgccggaaga tatggaaggc actgcaaagc ggcgactcat
    36061 ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct tttaaataat cgaaaaaagg
    36121 ggggaaagat aatggacgca tttgaggtac tggcaaataa aggcgctgaa ctggtagcac
    36181 agcgtgataa aaccgccaac gaaggggaac gcgcactgct aaataaacag attaaagcga
    36241 tccgtatggc ccagtttaaa ttaatcagta atgaagttat cgaaacaatt aaaccgcaga
    36301 tcccacaaat cgttgctgac gctattaaag ccgccggact ggagaaacgc attgccgggc
    36361 taaaaacgaa cgcagagaaa atggagatgt acaaacagag cggtgctccg aatcccgatg
    36421 agtacttctc accggatgtt gagtttatgg cgcaggttga ggaacgtctg ggtagctgtc
    36481 tcaccgagga acagcgtcgc tattttgatg gagtggatag ttcggcaggt attgacctga
    36541 actcgtattt cggtcgtgaa attgagcatt ttgatgccgc cccgtctatg ccggagccag
    36601 aaccagaaat ggccaccgat cctgaagctg agcaacggaa acgcgtattc aatgcgatag
    36661 ggattcggta ctgatcggat aaaagaaagc cgggaattac cccggctgac ttaacaatgt
    36721 gaccaatgaa caaatcggac aagacaaaca ggataagcag tctcgaaaca atgttcggga
    36781 actatattag cacaagtccg gagaaaatca tcatgttggc actacgcatc atgcagggca
    36841 tcgctaagac cctcgcagaa catgtcttag acctgaaaca ttcgccactg agcaaacagg
    36901 ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc gcttggaact atcaataaaa
    36961 tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga ggaaatggag ttcatcaggc
    37021 gtttaattct cattcgcaga gacattcata gccagttaca cagcgtggga atcgatatta
    37081 atgatgggac gggcgattaa ttttcggaca agtactatgc gcgcgcgagg agctgaacat
    37141 gaagcaagat attgcagaca ggctggagat actcgaaggc cagcgggcag aagcgaaaca
    37201 actacgtaag caggccagaa gagcgcacag gaacaacgaa gcagagttac taaccaaata
    37261 catcagtttc accaattact gcatctacga gtgttacaag gaagatgcag aggactggct
    37321 ggattcattg ccagaacagt actgagcaga ataacaccac gtcatgttgt tctgaggtga
    37381 gcatatcacc tctgttcagg tggccaaatt tagtgtgcca cttcagcatc accgttgaaa
    37441 tcacaataac gatttttttg taaatatgct ctcacatatg taggaataga ccaggttggc
    37501 attgaaacac atctaccatg accataataa agtaattttt tgatatgttt atggttttat
    37561 attaattaaa atatatctaa atttgatagt tttaaaattg caaaatccag attgtgtttg
    37621 taatgtttaa tctgattgta ttgagtggcg tttattatga aaaataaatc aggtaattct
    37681 tgttatagag aagcagtcga tattcatagc tcacttgagc tttcatttat attgcataaa
    37741 tatcatttgc gtgtagatga gaagaaatct cgaaatgtat tcagcgccat acgtgatgag
    37801 ctcatccttg atggggattc acgacaaaac ttagctactt tctgtcaaac atggcttgat
    37861 gatgaaatcc gtgagttgct taatttagct attgataaaa acatgattta taaagatgaa
    37921 tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta tgctttccga ttcgtggagt
    37981 ttccccgatt cgcgacacac cttaggttgc tcaaccattg gttcatctga agctgctatg
    38041 ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac gcgaagtaca aggtaaatca
    38101 accgaacctg atttgcgatc ctgtgcaaat atgctggcat aaattctgcc gttactgtga
    38161 tgtgaaattg tgtgaaattc cacttgatga tcatcacctg ataatgaatg ctaaagaggt
    38221 tttgaagcat gtcgatgaaa acactattgg tgtcgttacg actttaggta ttacttattt
    38281 ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt aaagttaata ttagttttta
    38341 tatttacatc gtaattatat gtttctattt taaaaatgtt cgagagtata aaaatgtcta
    38401 atgctaatat cgcacccaaa agtaagaaaa tgacagtggg gacactcgct atcatgaata
    38461 taactgctgt agtaagtcta cgatttatac cttctgaagc agaatacggc ttaggtgcca
    38521 ttttttacta tacctttgct gcaattatgt ttttagtacc tgttgccttt gttgcggctg
    38581 agctagctac aacatatcca caaaaagggg gggcatttcg atggatagga gaagcatttg
    38641 gggctcgctg gggttttgta ggaatgctta tgacctggct acaagtcatc ccctactttc
    38701 caacagtact gacatttggt gcagtctcgg ttgcatttat tgatgttgat atgggggtgg
    38761 cagaatcaat tgccagtaat aaatggtata ttttattttt cgtactattt gtttactggg
    38821 gagctatttg tgttgcatta cgtggcgttg gtattttctc gcaggtatct aagtggtgcg
    38881 gtattatagg gacaattatt ccagctatta tccttgttat tcttggtttc tcctatctgt
    38941 ggttcagcgg taaaccagcg gtgatcccat taggttgggg tgatcttttc cctaatatag
    39001 ctaacttcca gaatattgtt cttgccgcca gcatatttct tgcttatgca gggatggaaa
    39061 tgaatgcggt ccatgttaac gatttagata atccaactaa aaaataccca attgcaatta
    39121 ccatcgcttc tataggtact attgcaatat tcctgctcag tactttaggg gtcgccttta
    39181 ttattcctac agataaaatt agtctaactc aaagtttgtt ggttgcctac gatatgctgt
    39241 tcgaatgggc tggagtgcca tggcttggct ctgtgatggc atttatgcta gcaattggcg
    39301 tactgggtgg ggtggttact tggattgcag ggcctaatac aggtgtatta gcgatcgcga
    39361 aagctggtta tttacctaag tttttccaaa aaaccaatcg tcatggaatg gggcaccatc
    39421 taatgtttgt acaaggtata attgtctcag ttctttctgt tacttttgtg ataatgcctt
    39481 cagttcaagc agcttttcag attttatcac agttaacagt tatgctctat ttagttatgt
    39541 atatgctcat gttcgctagt gctatctatc tacgacattc tcaacctgat acaaaacgcc
    39601 catatcgagc ccctgcttta tttttctggg caacacttgg attcttaggg tcttttctgg
    39661 cttttgtatt aagttttatt ccgcccgagc aaatcccaac tggtagtcca accagctatg
    39721 ttctttattt gttagtatta gttgccctat ttttattaat tccaatatta atttacgcct
    39781 tccgaaaatc tgactggaaa gatccaaact ctgatttcga accattttct tgggaaaaaa
    39841 gaactagtaa ttaaattttc cataagctac aaaagatgag gttaaatatg tttagtgctg
    39901 aatctccaat tggtgtaata gactcaggtg tcggtggatt gacatcggta aaggaaataa
    39961 ttaatttgct tcctggtgaa gatattatct attgtggaga taatatgaat gcaccttatg
    40021 gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa aatgctgact tttttacagt
    40081 caaggaatgt caaactagtt gctgtcgcat gtaatactat atcctcaact ttggaatccg
    40141 aagaatacag cggatatgct aagagctttc cgtttcctat cttatccatc attgaacctg
    40201 cagtagaaga tgtgattcgc cagcaatata aaaatgttgg tattatcgct accgagttta
    40261 ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt gaattcaaca ataaatgtat
    40321 ttggagaacc aagcaaaaat cttgcaatgt taattgagga aggtaatctt aatgctcctg
    40381 caatcttgaa tgacatcaaa aaacatgtta accatttaat ttccctccat ccagtaaatg
    40441 aaattattct cggctgtact cactatccaa ttgttcagaa tttttttgaa gcagttgcac
    40501 cggatataaa atttattaat cctgcacatg atcaggctat ctctattaaa aatcacttgg
    40561 gacagctaaa tttattaaac tcctcaaata taggaacttt gcaaataaat actagtggaa
    40621 gtatggagat ctacaaaacg gtactagggg aattgagcat tacgaaacct catacattca
    40681 gtatcaggca gttttaaatt tatccggtac accgtaaaat cccgtccttc tgaactgcac
    40741 cccaaaagtt ggacaccaac tgagtaaggt gcattttatg gacaaaccca gttacatact
    40801 gggtagtgca tccaattagt agaacatgtg tttttcgata aacgctccga tcactttttc
    40861 gtggatctca actgagcgtg agaagcagat tgttttacga cccaaccgct taatgcgggt
    40921 gcgtagcgtc agattattac gctcaatgcg ttgggtgaat attttgccgg tcagatgctt
    40981 attcttcggc acctcccggc catagctgcc ccagtcatcg ctggtcagca tgccgatgtt
    41041 gaagggtgta agcagtgcca gtagctcccg gcacgtttga tcggttcggg gaccaaaagt
    41101 gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac ccgagccaat gttgccgggc
    41161 tttactgcca acgtagctcc attgctcatc aagctcgcag ataaacgcca catcagcatg
    41221 ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt ttaaagtccg gatgacggtg
    41281 ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc cggcaccatt aaaggccatt
    41341 tcagttatca gctctttcat acccggctta cgtgcttgat aagtataagt gaactgaaac
    41401 acacggtggc agtcacggca gcgaagtctg tcacggcctt tagggttctg accatggcgg
    41461 taaacctgag ctgactgaca acggggacaa tgaatattaa cgctggtcat gagataacct
    41521 caaaagcccg tattatacat cagattcaac taattagagg catcacccat ccagcattat
    41581 aaaacatact aacaataact aaattataat aatcacaact tacaatcaaa taataaatgc
    41641 ataagattaa caatgatttt ttcgggttat atttatctaa aaaagcagaa cattatgtaa
    41701 cttattattt tctaagcgaa aatttattgt gcggtcagac taacaaactt gcgtaccacc
    41761 cgaagaacat gcgaagattt aagtattctt tcttttttgg tttttatttt tcattttctc
    41821 ttttcttctc tttccggttc tgttctggtt ttggtttttt cctttttatt tttattcttt
    41881 cttcagggtt atgctaaaac accaaaacat ttttcaccac aaaagcatcc aatcatcttg
    41941 ccagaccaat caccttaacc ggatacactg ccggatataa tcccactaac acccagacac
    42001 caagcaccat acctgataac taccgtattc aggaaggaaa tgcgagtgat ttcaccgtca
    42061 ccggcacaac cgatccgcca aaaagaggcg taccaatgtc gatatagtcc cccgcgcgga
    42121 caatcacttc gtcaatgact gccaacggga gttgttgtag ctgtgggcgc aacaacatgc
    42181 ccagagcttt accaaagtcc tgcccggcca ccaccagcag gggatgcgga ttcggaaaac
    42241 gcgcgacgaa atcgaccagc gcgttgatga ccgtcagaac cgcggcgtaa cgcacaggca
    42301 gcgaggcggg aagcgccagc acgtacacgt cagttttggg atcaagatcc agctgaagca
    42361 gcgcctgttg ccaggcactc accagatccg tttcatcaat cgggatcgcc accggcaaat
    42421 tgcgcagcgg cagttgtacg ccctccagcc agattgtgct gccagagagc gaaagggtat
    42481 gtgcacccgc gccaatcacc gtggagcgta cggtttgcgc cggaaactgc acattcatct
    42541 cacgcaggcg cggatggtca tgcagcgccg ttgccagaag tgggccaata tcggcaaaac
    42601 agaacgggtt ggcgggctgg tggcgataac attcgcccac gcctccagaa agcgtaatga
    42661 tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat caatgcctgc gcgagcggcg
    42721 aaagcgttcc gtcaatcact tcgacaatca gcgccgccat ccgccgggta acctgcacca
    42781 gctgcgcgcc ggtcagcgaa cgggcgtcag tgcctgcacc gaagcactca tccacaatca
    42841 tctgccccgg tttatgagcg taaaccacgc gcccctggct gtcggtttcc agcaggcgac
    42901 caccgacgtt gagacaggca gtgccgctga tttttccggc atcgaacagg gcgtagttcg
    42961 cggtgccacc gccgatatcg atattcagta cccgacacag ccgttgttca gaaagggttt
    43021 gcgccccggc tccgtgaccg gcgatcacgg attcgaggtg cggcccggcg ctggcgacga
    43081 caaaatcgcc cagcgactgt ttttccttca tcactttttc catcagctgt gcgaccaggc
    43141 tttcggtaaa ctggccttcc ggcagctggg caatgatggt ttcgcggatg cgctggcttt
    43201 cggttttgtc ggctggcacg ctggcgggca cggacgccgt taccgggatc gattcatcac
    43261 attcggtgat ggtaaaaccc agtagatccg ccacttcacg ggcttccggg gtaataatgc
    43321 tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat
    43381 tcgctgtgat gagttttttc acctgcttcc tcctgtttgg tgagagatgc cagataatcc
    43441 accagttgct gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaaa
    43501 cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag catctggcat gtccgtttta
    43561 ctgatgacgg cgatttgccg tttgctgacg ccaatatcca gcaacccggc aggcaaccga
    43621 ctttccgggt catttgcgcc atggacatag atcagcatgt ctacatcctg tagcgtggta
    43681 attaaggcgt gataccagcg gggatggcta aaatattcac ccggcgtatc aatatcgccc
    43741 ttatcattaa attccacggc cagtgttttt ctggcgaggg tataattccc ctgcaacgca
    43801 ttaaataacg ttgtttttcc tgccccgacg ctacccacaa acgcaattcg tttcatgaat
    43861 gcctcgtatt aacttttggt catttcacaa agcgtataat ttaataagcg acctaaaccg
    43921 ctgacggttt gtgataacgc ttcttctact gcgcccaccg agccataaat caccaatgca
    43981 ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg ctttcagcgc taaatcaccg
    44041 gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc caattgcgcc cgcatccgga
    44101 acgccgattt tcttcgccag ttcctcaccg ggatgcgcga tgagatgcgc cagtgtgacc
    44161 tgtttgccgg gcacaaattc ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga
    44221 aaaactttcg tgtatcgtga caatttttcg tcagagtaaa taacaaaatt cggcaagcgc
    44281 gtttaaaagg tgaggtagat caataagaat ggagagatat ttactgttat gaataaactt
    44341 tcatatgcga cgcatgaata taaataaaat aatatcgaga tattttatat actgattttt
    44401 ttattattcg caaactcagt aataagcttt gctaatatat ttcaggaaaa ccgcttaccc
    44461 tgaatattca gggtaagcgt gagagttaag aatgaattac agcggttggg tttgcgcttc
    44521 aaccacggcc agcgccacca tattgacgat acgacgcacc gatgcgatcg gcgttaacac
    44581 gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga
    44641 aacgcgcagt aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt
    44701 ggcagaacct ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc
    44761 tgcatcgccg tgcatttcac catcaatcat cagatctggc gcacgatccc tgaccagttc
    44821 cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga
    44881 caacagagca acgcgcggct caataccaaa acgacggaca gtttctgccg ccatcaaggt
    44941 gatctccgcc agctcttctg cgtctggttc gtcattgacg taggtatcgg caataaaggt
    45001 gttaccactc ggcagcagca gcgcattcat tgcacctgcg gtgtgaacgc catcgcgata
    45061 accaaagaca tttttcacca cactaaaatg ttcatgataa tcgcccaccg taccgcagat
    45121 cattgcatcg gcttccccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat
    45181 cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc
    45241 ggtccagtac tctttaaagc gcggatcgga ttcgttattg acgatttcaa aatcaacgcc
    45301 cgctttgatc tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccgat
    45361 aaggatcggt ttcgccagtc ccagcgttac cagttcctga gtcgcatgca gaacgcgcgc
    45421 ctcttcccct tccggcagaa ctacgcgctt cggcgctttg cgagcctggg agaaaatcgg
    45481 cttcataaac aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa
    45541 atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc gcttttgcga ccgcaggagc
    45601 gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct
    45661 cagatcctga acgccatacg ctgaagccac cacttcactc tgttctgcat gggccagttc
    45721 tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg atggcggttg cgccaacgtc
    45781 cagcgcgcca cggaagatga acgggaagca aagaacgttg ttcacctggt tcgggtagtc
    45841 ggaacggccg gtacagatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat
    45901 ttccggttcc gggttcgcca gcgccaggat cattggtgcc cgagccattt tcttcaccat
    45961 ttcctgggtc agcactttcg ggccggaaca gcccaggaaa atatccgcac cttcaatcac
    46021 atcatcgagg gtacgtttgc cgtcatccac caccgcatat gcggctttgg tttccgccat
    46081 atttggctca cggccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt
    46141 ttgcagcccc agcgctacca gcaggttcat acaggcgatt gctgcggcac ccgcgccgga
    46201 aaccaccatc cgcacgtcgg agatgttttt ctccaccacg cgcaagccgt tgaggatggc
    46261 ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg
    46321 ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat
    46381 gccgccgaag gtcggttcga gcgcggcgac gacttcaata aatttgtccg ggtcgagttc
    46441 atcaacttca atgtcaaata catcaatccc agcgaatttc ttaaatagaa cgcccttgcc
    46501 ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc
    46561 gttagagatc accgcaacca ggttaccgcg cgcggtatat ttgtaggctt ttagcgggtc
    46621 tttttcgatt tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg
    46681 ctgcgttgcc agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg
    46741 gaaatcaagt gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt
    46801 tcacaaataa agtgtgtggg caacagcccc tgcccacaac gtggcgcaca ttattaccct
    46861 gccggagtct acagactttg agcaagtcca aactctcacc attaatataa tgttttggta
    46921 ataatcctat aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg
    46981 cttttttgat gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac
    47041 tttacttttc aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact
    47101 gtcgtggcag acagctgcga tattgagtcc attcgccatt atcatcccca ggatgccacc
    47161 actaatcctt cgctgttact caaggctgcc ggattatcac aatatgagca tttaatagac
    47221 gatgctatcg cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg
    47281 tgtgacaaac tggcggtcaa tttcggtgct gaaattctca aaatcgtacc cggttgcgtg
    47341 tcaacagaag ttgatgcacg cctctctttt gataaagaaa agagtattga gaaggcgcgc
    47401 catctggtgg acttgtatca gcaacaaggc gttgagaaat cacgcatttt gatcaaactg
    47461 gcttcgacct gggaaggaat tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc
    47521 aacctgacgc tgctgttttc ttttacccag gcgcgggcct gtgcggaagc aggcgttttt
    47581 ctgatttcgc cgtttgtagg gcgtatttat gactggtatc aggcacgcaa gccgatggac
    47641 ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta cgactactat
    47701 aagcaacacc actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc
    47761 ctcgccttaa ccggctgcga tcgactgact atcgcaccga atttgctgaa ggagctgcag
    47821 gaaaaagttt ctccagtggt acgtaaatta atcccgcctt ctcagacgtt cccacgccca
    47881 gcacccatga gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa
    47941 aaactgtctg aaggcattcg tctgttcgct gttgatcaac gcaaactgga agatcttctt
    48001 gccgccaaac tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc
    48061 gattcgcgca ctcagtatgg atgctgtaca aaaagccaat tctggtcatc ccggcgcgcc
    48121 gatgggcatg gctgatattg ccgaagtgct gtggaacgat tttcttaaac ataatcctac
    48181 cgacccaacc tggtatgatc gcgaccgctt tattctttcc aacggtcacg cgtcgatgct
    48241 gctctacagt ttgctacatc tgaccggtta cgacctgccg ctggaagaac tgaagaactt
    48301 ccgtcagttg cattcgaaaa ccccaggcca cccggagatt ggctatacgc caggcgttga
    48361 aaccaccacc ggcccgcttg gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga
    48421 gcgtacactg gcggcgcagt ttaaccagcc agaccatgag atcgtcgatc acttcaccta
    48481 tgtgtttatg ggcgacggct gcctgatgga aggtatttcc cacgaagtct gttcgctggc
    48541 aggcacgctg ggactaggca agctgattgg tttttacgat cacaacggta tttccatcga
    48601 cggtgaaaca gaaggctggt ttaccgacga tacggcaaaa cgttttgaag cctatcactg
    48661 gcatgtgatc catgaaatcg acggtcacga tccgcaggcg gtgaaggaag cgatccttga
    48721 agcgcaaagc gtgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg
    48781 ttcgccgaat aaagcaggta aggaagaggc gcacggcgca ccactggggg aagaagaagt
    48841 ggcgctggca cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaagagat
    48901 ttatcacgcc tgggatgccc gtgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa
    48961 gtttgccgcc tataaaaagg ctcatccgca actggcagaa gagtttcccc gacggatgag
    49021 cggtggttta ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttacaggc
    49081 aaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat acgcttaacg cttacgggcc
    49141 gatgctgcct gagttgctcg gcggttcggc ggatctggct cccagcaacc tgaccatctg
    49201 gaaaggttct gtttcgctga aggaagatcc ggcgggcaac tacattcact acggggtgcg
    49261 tgaatttggc atgaccgcta tcgccaacgg catcgcgcac cacggcggct ttgtgccgta
    49321 taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac gccgcgcgga tggcggcact
    49381 gatgaaagcg cggcagatta tggtttatac ccacgactca attggcctgg gcgaagatgg
    49441 tccgacgcac caggctgttg agcaactggc cagcctgcgc ttaacgccaa atttcagcac
    49501 ctggcgaccg tgcgatcaga tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca
    49561 caacggaccg acggcactga tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc
    49621 ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc
    49681 agatattatt ctgattgcca ccggttcaga gatggaaatt accctgcaag cggcagagaa
    49741 attagcagga gaaggtcgca atgtacgcgt agtttccctg ccctcgaccg atattttcga
    49801 cgcccaggat gaggaatatc gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc
    49861 ggtggaagca ggtattgccg attactggta caagtatgtt ggtctgaaag gggcaattgt
    49921 cgggatgacg ggttacgggg aatctgctcc ggcggataag ctgttcccgt tctttggctt
    49981 taccgccgag aatattgtgg caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg
    50041 gtgatcgccg gatgctgtat gttcccctca ccctaaccct ctccccagaa gggcgagggg
    50101 acggtccgtg cgctttttag ggccgcatct gcgattttgt ttaacgggtg atccacaacg
    50161 tgggcctggc gtctggccca tgccagttat cgcaggtagg ttctgcggcg taacgcacca
    50221 ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc
    50281 gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt
    50341 ccagttcatt cgtctcctgg ccgttgttga acggcaaacg caaccgaacc gggcgcgagg
    50401 ctagcggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct
    50461 cacagctaat catcatcagt gctttatcat cggtcagcgc agtgacattc acctcacgac
    50521 gtaacggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat
    50581 cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt aaccaccgcg acctctttca
    50641 gcgcaggcgc gggcggtacg ctgagaggcg gttcgtcccc tttcttgatc cacgcggttt
    50701 cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccgctca
    50761 gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga attatctgca
    50821 aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact
    50881 tgtcaccact taatgccaac ggctcaccat ctaatagcag ccgtggcgct atctcccctt
    50941 cagacgcatc cggcgacttc aacccaccgc gctcaatacg taaaacggca tcggtatgcg
    51001 ccccagcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca
    51061 cacaaaaatt ttgattatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg
    51121 ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc
    51181 gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc
    51241 aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat aataaacaac gaaatattat
    51301 tgctattttt cagtccatta aatgtgacat ttgcaaatag ttaagcaata acaccgtctt
    51361 accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag
    51421 cacttcaata tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta
    51481 ttcggcgata aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa
    51541 taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca
    51601 cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc
    51661 attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgc tctttttctt
    51721 cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg
    51781 gataacttcg ccatctttgc gcgtgagatc gtaagtaatg ttgtgcaggg tgaaatagtt
    51841 atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg
    51901 cgaaatgaac aattatatta cgctgtgaag tcgggtttgt ctgcgcaggc tatcagtgag
    51961 atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga cgtccttctg
    52021 ccattgcggt aaccaccaga tccgcaccac gcacggcgtc gccaccggcg aagattttcg
    52081 gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt
    52141 ctaccgttac accgtgcgac tccagccacg gcatcccgtg tggattgaag ccaaacgcca
    52201 taatcaccgc atcggctggc atgacaaatt cactgccctc aatcggtaca ggtcgccgac
    52261 gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaattccg caaacgtgac
    52321 cttgctcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt
    52381 cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt agcttcatca cgacgataag
    52441 cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc atcgcggtgt
    52501 cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta
    52561 gctcttcgag ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa
    52621 cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa
    52681 cgaagaccgc gtcgtattgt tccaaaagca aatccaaaga gacatcttta cccacttcac
    52741 aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg
    52801 atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc
    52861 gatcgtacac cgtcaccgcc acgccattgc gggtcagaac atccgcacag gccagccctg
    52921 ccggacctgc cccgataatc gccacccgct tgtccacttt ggtgacatga cttaagtcag
    52981 gacgccaacc tttcgccaac gcctgatctg aaatatagcg ttcaatgttg ccgatagtta
    53041 ccgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa
    53101 cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga
    53161 tgtttccggc tttcaccagt tcaatccact gcggaatatg gttatgcagc gggcaggtcc
    53221 attcacaaac gctgtgctcg ccgaacttaa ggcagcgcga ggcttcccgt tgtgcctggt
    53281 cggcgcgaaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt
    53341 tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca
    53401 tttcttgtgc agcggtactg gcatgccacg gttgatgctc ctgacgtgcg gtgcgcaagc
    53461 ggcgggattt cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg
    53521 ggcaattctc gacacaggca ggaccgtttt cgcgccctgc acaaaggtcg catttatgcg
    53581 ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaacg
    53641 gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt
    53701 catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat
    53761 ggtgacaggt cactgcacta cgttgctgct gatgtttgat aaccgtaatt cggggatgaa
    53821 aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac
    53881 aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct
    53941 tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag
    54001 gggaaccggc aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag
    54061 atcaaataat cccatcactg actaaattgc gtttcagcga actggaacat taatgatttt
    54121 ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct
    54181 ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga
    54241 gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg cagagttacc
    54301 gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc
    54361 agcaggcact gcattcaccg gtattaacca acctcaacgt ctggtatgtg ccagaagcag
    54421 taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca
    54481 agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt
    54541 tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc
    54601 tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgcgccgc atacgccatc
    54661 aggtggttgc cccgctgaat cagctggtta ccgccagtca gcgtattgaa cacgggcagt
    54721 tcgactcgcc gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct
    54781 ttaaccaaat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag
    54841 aaaagacccg cgatctccac gagaccaagc gtcgtctgga ggtgttgtat cagtgcagtg
    54901 ttcccaggcg ctgaacacca gccagattga tgtgcattgt ttccgccata ttttgcagat
    54961 tgttcgcgac aatgaagcag ctgaatatct ggagctgaac gttggggata actggcggat
    55021 tagcgaaggg aagccaaacc ctgagctgcc aatgcagatt ttaccggtga caatgcaaga
    55081 gacggtttac ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag aaccgctgct
    55141 taacagtgtt tcatcgatgc tgggacgcgg tttgtacttt aatcaggcgc agaagcattt
    55201 tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc cgcgaattgc acgactcgct
    55261 ggctcaggtg ctttcttact tacgtatcca gttgacgtta ctgaagcgtt cgataccgga
    55321 agataacgcc accgcacaaa gtatcatggc cgatttttcc caggcgttga atgatgctta
    55381 tcggcagtta cgcgagctgt tgactacttt ccgcctgacg ctgcagcagg cggatctccc
    55441 ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa accagcgcta aactgaccct
    55501 cgactgccgt ctgccaaccc tggcactgga tgcgcaaatg caggtgcatt tgttgcaaat
    55561 tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag
    55621 ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc cgtgataacg ggattggtat
    55681 cggtgaaccg aaagaacccg aaggtcatta tggactgaat atcatgcgcg aacgcgcgga
    55741 acggctgggc gggacgctga ctttttcaca accttccggc ggcggcacgt tagtgagtat
    55801 tagctttcgc tccgcagagg gtgaggggaa ggggaacacg ccggctgagg tgtaaataat
    55861 taccctcaca ccggccctct ccctttaaag gcgagaggga aaaacgtgcc catattgacc
    55921 tgtaagcgag tgtgtcagca atctcatgat aatttacatt aactcctttt tttctccacg
    55981 attggctcgt accttgccgc tacagtgaag caagtcaagc ctacaacaat acgcagaaac
    56041 acgaggtcct cttttaatgg cgaatttctt tattgatcgc cccatttttg cctgggtgct
    56101 gacaatcctg ttgtgtctga caggtaccct ggcgattttt tcattgcccg ttgaacaata
    56161 ccccgatctc gcgccaccga atgtgcgagt gaccgctaac tatcccggcg catcggccca
    56221 gacgctggaa aacaccgtga cccaggttat cgagcaaaat atgaccggcc tcgataatct
    56281 gatgtatatg tcatctcaga gcagtggcac cggtcaggca tctgtcactt taagttttaa
    56341 agcaggcacc gatccggacg aagccgtgca gcaagtacaa aaccagctgc aatcagccat
    56401 gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa ccggcgatac
    56461 caacattctg accattgcct tcgtctctac cgatggctcg atggataaac aggatattgc
    56521 tgattatgtt gccagtaata ttcaggaccc gttaagccgc gtgaatggcg tcggggatat
    56581 cgatgcctat ggttcgcaat attccatgcg tatctggctg gacccggcga aactcaacag
    56641 tttccagatg acggctaaag atgtcactga tgccattgag tcacagaacg cgcagattgc
    56701 ggttgggcaa cttggtggta caccttccgt cgataagcag gcgctcaacg ccaccattaa
    56761 cgcccagtca ctgctgcaaa caccagaaca gttccgcgat atcaccttgc gggtgaatca
    56821 ggacggctca gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg cggagaaata
    56881 cgattatctt agccgcttca atggtaagcc agcctccggg ttgggggtaa aactggcctc
    56941 cagcgcaaac gaaatggcga cagcggagct ggtgctcaat cgtctcgacg agctggcgca
    57001 gtatttcccg cacggactgg aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc
    57061 ctccattgaa gacgtggtga aaacgctgct ggaagctatc gctctggttt tcctcgttat
    57121 gtatctgttc ctgcaaaact tccgcgccac gctgataccc actatcgccg tgccggtggt
    57181 gttgatggga accttctccg tactttacgc cttcggttac agcgtcaaca ccttaaccat
    57241 gttcgcgatg gtgctggcga tcggtctgct ggtggatgac gccatcgtgg tggtggaaaa
    57301 cgtcgaacgt attatgagtg aggaaggact cactcctcgc gaagccacac gtaaatcgat
    57361 ggggcagatc cagggggcac tggtcgggat tgcgatggtg ctttcggcgg tatttgtacc
    57421 aatggccttc ttcggcggca ccaccggtgc catctatcgc cagttctcta ttaccattgt
    57481 tgcggcgatg gtgctgtcag tactggtagc gatgctcctc actccggctc tgtgtgccac
    57541 gctacttaag ccactgaaaa aaggtgagca tcacgggcaa aaaggttttt ttgcctggtt
    57601 taaccagatg tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga aaattctcca
    57661 ccgtagcctg cgctggattg tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt
    57721 cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgg ggcatgttta ctacctcggt
    57781 acaattgccc agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa
    57841 atattacttc acccatgaaa aagacaacat catgtcggtg tttgccaccg ttggttctgg
    57901 ccctgggggt aacgggcaaa acgtggcgag aatgtttatc cgcctgaaag actggagcga
    57961 acgcgacagt aagaccggca cctcgtttgc cattatcgag cgtgcaacga aggcgtttaa
    58021 ccaaattaaa gaagctcgcg ttatcgccag cagcccacca gcaattagcg gtcttggtag
    58081 ttctgcaggt tttgatatgg agttgcagga ccacgctgga gcgggtcacg atgcgctgat
    58141 ggcagcacgt aatcagttgc tggcgctggc ggcggaaaac ccggagctaa cccgtgtgcg
    58201 ccataacggc ctcgatgaca gtccgcagtt gcagattgat atcgaccagc gtaaagctca
    58261 ggcgctgggc gttgatatcg acgatattaa cgacacactg caaaccgcct ggggttcgag
    58321 ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc aggcagctgc
    58381 gccgtatcgc atgctgccag atgacatcaa tctctggtat gtccgaaata aagatggcgg
    58441 catggtgccc ttctctgctt tcgcgacctc acgctgggaa acaggctcgc cgcgtctgga
    58501 acgctataac ggttattctg cggttgagat tgttggggaa gccgcaccgg gggtcagtac
    58561 cggtacggcg atggatatta tggaatcgtt agtgaagcag ctgccaaacg gctttggtct
    58621 ggagtggacg gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc cggcgctgta
    58681 cgccatttcc ttgctggtgg tattcctgtg tctggcggca ttgtatgaaa gctggtcggt
    58741 accgttctcg gtaatgctgg tcgtgccgct gggggtaatc ggcgcgctgc tggcaacctg
    58801 gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg tcattggttt
    58861 atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac gagatgaacc aaaaaggcca
    58921 tgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga ttctgatgac
    58981 ctcgctggca tttatcttcg gcgtattgcc aatggcaacc agcacgggtg ccggttccgg
    59041 tggtcagcat gcggtgggta ctggcgtaat gggcgggatg atttcagcta ctattctggc
    59101 tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc cgcttcccgc tgaagccgcg
    59161 cccggaataa gcaataaaaa aggcgacatg ccaatgtgtc gcctttttca actttccgat
    59221 taagaacctg ctcagcgggt tcttgctgtt tatactttgt ctcaggaatt acttacgaag
    59281 cataacttcg ataaagtctt tccagttccc cagttcacgt tcaatcataa caacctctct
    59341 tataattatg ggtattctac ggaaacaata taccgtggtg aagctaattt actcgattgc
    59401 tgcgatgact acctccgggg gacaaacctt atgtaaatac tatggtcctg cggtgataat
    59461 ttgtatgtga tacacagcaa catttcgaga tattcatacg gcatctaata ctgatttaat
    59521 tctggttaaa atacagacag ataacaagat gaatattctt aatgtttacg ttaaaaatgt
    59581 ttaatattat ttaatagttg ttaatttgaa tacttcgata atgttatatt ttctgataat
    59641 catttgcagg caaaatgttt tcacccttaa atgagtattt attctcataa atcgaaaaag
    59701 gattcattat ggttacactt tacggcatca aaaattgtga caccattaaa aaggctcgcc
    59761 gttggctgga agccaataac atcgactatc gttttcatga ttaccgcgtc gatgggctgg
    59821 acagcgaatt attgaacggt tttatcaacg aattaggctg ggaagcgtta ctcaacaccc
    59881 gtggtacaac ctggcgtaaa ctggacgaaa ctacccgcaa taaaatcacc gatgcggcct
    59941 ctgcggcggc attaatgact gaaatgcctg caattatcaa acgtccattg ctctgcgcgc
    60001 ctggtaagcc tatgctgctg ggtttcagtg attccagtta tcagcaattt ttccatgagg
    60061 tgtagtctat gtcgtgcccg gttattgagc tgacacaaca gcttattcgc cgcccttccc
    60121 tgagtcctga tgacgcagga tgtcaggctt tgttgattga acgtttgcag gcgatcggtt
    60181 ttaccgttga acgcatggat tttgccgata cgcagaattt ttgggcatgg cgtgggcaag
    60241 gtgaaacgtt agcctttgcc gggcataccg acgtggtgcc gcctggcgac gccgatcgtt
    60301 ggatcaatcc gccgtttgaa cccaccattc gtgacggcat gttattcggg cgcggtgcgg
    60361 cagatatgaa aggctcgctg gcggcgatgg tggtagcagc agaacgtttt gtcgcacaac
    60421 atcccaacca tacggggcga ctggcatttc tgatcacttc tgatgaagaa gccagtgccc
    60481 acaacggtac ggtaaaagtc gtcgaggcgt taatggcacg taatgagcgt ctcgattact
    60541 gcctggtcgg cgaaccgtcg agtatcgaag tggtaggtga tgtggtgaaa aatggtcgtc
    60601 gcggatcgtt aacctgcaac ctcaccattc atggcgttca ggggcatgtt gcctacccac
    60661 atctggctga caatccggta catcgcgcag cacctttcct taatgaatta gtggctattg
    60721 agtgggatca gggcaatgaa ttcttcccgg cgaccagtat gcagattgcc aatattcagg
    60781 cgggaacggg cagtaacaat gttattccgg gtgaactgtt tgtgcagttt aacttccgct
    60841 tcagcaccga actgactgat gagatgatca aagcgcaggt acttgccctg cttgaaaaac
    60901 atcaactgcg ctatacggtg gattggtggc tttccgggca gccatttttg accgcgcgcg
    60961 gtaaactggt ggatgcggtc gttaacgcgg ttgagcacta taatgaaatt aaaccgcagc
    61021 tactgaccac aggcggaacg tccgacgggc gctttattgc tcgcatgggg gcgcaggtgg
    61081 tggaactcgg gccggtcaat gccactattc ataaaattaa tgaatgtgtg aacgctgccg
    61141 acctgcagct acttgcccgt atgtatcaac gtatcatgga acagctcgtc gcgtgatgag
    61201 tggttctgca agaggaaata agcatggact ggctggctaa atattggtgg attctggtga
    61261 ttgtcttttt ggtaggcgtc ctgctgaacg tgattaaaga tctcaagcgc gtcgaccata
    61321 agaaatttct cgccaacaag ccggagcttc ccccgcatcg tgatttcaac gataagtggg
    61381 acgatgacga cgactggccg aaaaaggatc aaccgaagaa gtaagctgag tgctcaaccg
    61441 taggccggat aaggcgttaa cgccgcatcc ggcaatggtg aacgatccct gatgcgacgc
    61501 tcacgcgtct tatcaggcca ggtaatctgt cttttacatc atttcaatca catcatcatc
    61561 gccaggctta ccgccactta gcgcttggtc gaaataatgt ttcggaatgg tataacgcaa
    61621 gtgatccagg gcaaattgca tactgcgatt gtcgatcgca tggcctaaat cctccacgat
    61681 atccagcgtc acgtcaccac cggcactgat taacgcttcc tgcgccgcaa ctgcatgcgc
    61741 cagatcgata accggatctt caccaccatg aatcaagtga attgtcgtgg ctgtagacgc
    61801 ggtctccggc aggctcgcat agcgtccgtt aaaggcaatg acgcgcgaag caaggcccgg
    61861 ttcggcttta atgctctcca gcgccataat cgcgccttgt gagaaaccaa ttagcgccgt
    61921 ggcgttagcc cctactccgc tctgtttctg ccagtagcgt accgtttcaa taaacgtcgg
    61981 catgatggca tccacacgcg cctggcgatt atcttccgta ataccctgca ctgaaaacca
    62041 ctgacgcccc gccggattac cgctcggctc cgcgccaccg acactcacca ccagcgcatc
    62101 aggaaacagc ggtgcaaacc agctgcctat ttcccccatt gccaccgggt tatccccgac
    62161 accgtggaaa agcagtaaca actgttgtgc aggtttatcc gggctttgaa caacaaaatg
    62221 gtcatgtttc atggcgaact ccttaactga tgtcattaat tttacgccgc tgactcacaa
    62281 tgaccatggg agaaaattga aggagtcagt gaaaaaattg ccattgcgtt atgcgatcgc
    62341 gcagacgctc agtgcgaacc tcatttagtg cgaacagcgc ctgcgcggcc tcttcccgct
    62401 ggcgaaccag taacatcttg cggcctgaaa gtttaagtgt ggtacataac tgcgcgtcac
    62461 tggcgttttt ctgtaaacgc ccacgcagcg ccggtaatgc cagttcactg gtttgtagca
    62521 gacgcaataa gcaacctaac gatgttaata gcggacgatg agcgaaagca aaaccggcca
    62581 gttcaagcca gtcgtcgtca gaaaggatgg catcgtttag tggatcaaca ggaagcattt
    62641 cgccattcca ctgcgcgaga gcttgcgcat cgcgacgtaa acggtaatgc tcacgttcag
    62701 ccagctgttt acccgcatca ctcatcggta acagcgccat cgccgtatag caaccgctgc
    62761 tggcttcccg atgattaccc atccgcacca gcacaaaacc gcagcgttgc cagaaacgcc
    62821 ataactcccc agtgtaacca aaactcaccg aaagatagtc gaggtcatgc gtatattgca
    62881 aagctccagc aataagttgt cgccctgtgc cttcccgctg acgagccgga tgaactgcta
    62941 tccggctaac ccgccttccg cgcaatgtcg ccgccagtgg attgctgccg tgcgccgcca
    63001 gcgactgggc caccagatta ccccgcgggc gacgaaaacc tgcccatacc gcctgactga
    63061 gttcttgaga taatccgccc tcatccacca gccacaacgc cccggcaatc tcgttttcgc
    63121 cagccgcctg taaaaaatgt tgccctggtg catccatcat ccggcgtaaa tccagcggcg
    63181 aggtccggta gtgcgcacca gacaataact gataaacctt taacggcgtt tctggctcgc
    63241 ttcgccataa cgtttgttca aatgcggaaa tgacgatatt gccttgtggt gtatgggtga
    63301 agttttcatc gttaaaaacc agtgcctcac taaccatttt ttccagcggg catccctgtg
    63361 cccagcggat cggctgttgc agttcaaaac ggtgtaaatg cggaaagcga gcgcaaaatt
    63421 tcagcaaaaa accacgtccg gtaccttcgt agccctgcac cgtagtggtt aacaacgttc
    63481 gaggaaaacg cgataccagt tgatgcaaca acggcgcagg tatggctgcg gcttcgtcga
    63541 ccaccagcca gtcggcttgc tcatcgctgg ctaacaaggc atccggtgca ataaagcgaa
    63601 acttctcgcc cgcaaattgt gccagtacat ccgttgccgc ttttgcgggc gcggtgacaa
    63661 tcgcactgcc cgcaatacga gaaatgagtt gccctgccaa cgccgactta ccgcgcccac
    63721 gcgcagccgt taccgctgcc acgcccggcg gcatggtcag tagctgctgt aagagttgct
    63781 gttgttctgg ttgtggtgcg ccggtcgcgg ggtgccagtc agtacgggga gtaaaatgcg
    63841 ccaacgagaa cggctggttt tgccgccaga ggatagcgtc gttatccgcc gtaagtacgc
    63901 gtttgagatg ctggacaaaa tgcggcgtcg caatagggtc agggcaatca ctccagcgca
    63961 gcgagtcggc atcaggttgg ttttcccact cttcccatac agggagtaac aaaaccagcc
    64021 agcttcccgc tttcaacgtt ccgctaagtg ctgcaaaggc agcggcatca aagccgtggc
    64081 gggcgtcgaa taccgcatgc cggaactcgc gcccaagtaa agtttgtagc gccgagggtg
    64141 aacagtggtt ttcagcatct agctgcggcg aaatccacag ccagtcgcca ggtaaggcat
    64201 cacgcaactt aagagcatga tcaaaacacc aaccctcttc cccgctcaac accagcaagc
    64261 ggcagatccc ttcacgtttc atttgcgctg ttaatgtgta aagcgcagtc agttcagcca
    64321 tccctgcccc ggaaattaaa tgcttttacc aaaagtattg cattgtgccg gatcgccgct
    64381 gtcgaaacca cgtttaaacc agctgtagcg ttgctgagaa gtgccatgtg tgaaactgtc
    64441 tggtactact cgcccctgac tttgctgttg taaacggtca tcgccgatgg cctgcgccgc
    64501 attcagcgcc tcttccagat cgccagtttc cagaacgcct tgctgctgca tactatgccc
    64561 ccagacaccg gcaaaacagt cggcctggag ttccatacgc acagataagc ggtttacttc
    64621 cgcctgcgtc gcgttttgtt gcagttgacg aactttcggc tcgatgccta acagtttctg
    64681 cacatgatga ccgacttcat gggcgataac gtacccctgg gcaaaatcgc catccgcgcc
    64741 aagtttgtct ttcatgtcat catagaagga gagatcgata taaactgtgc catccgccgg
    64801 gcaatagaac ggccccataa tggactggcc cgccccgcag ccggtgcgcg tcattccacg
    64861 gtacatgacc agcttcggtt gctgataggt cttacccatc ttttcgaact gttgtcccca
    64921 ggtgtcttcc gtggttgcct gaatcaccga ggtgaatttt gcggcttcgt cttcattagg
    64981 gctaattgac cgcgttgatt gttgttgaga aaccggctgc ccggtcatca acccggttaa
    65041 atcaacgcca tagtagcctg caaccagcac gactatcagt aaaatcagcc cgcctttacc
    65101 gctcggcaga cggaaaccgg gaccgcccat tgacggaccg ccagagctgt tgcgcctgtc
    65161 ttcaacattg tcactttcac gtcgcccttg ccaacgcata gatacctcaa caatatattc
    65221 attatagaaa tgatcgtagg tggttcagag gaagattacc acaggaaagc gggatgaaat
    65281 ggcggaggaa tgagcgttca atgcatatat tccaagagcg gttgtcttgc cgtgccagct
    65341 gcacggcaag atgatgaaca gaaaaatcag tccagctgta cacccaggcg gcgggcgacg
    65401 gcttcatagg cttcgatcag gccaccgagg ctctggcgga aacggtcttt gtccattttc
    65461 tccagtgttt ctttgtccca caggcggctg ccgtccgggg agaactcatc gcccagtacc
    65521 acttcacctt tgtacagacc aaactccagc ttgaagtcga ccagaatcag accagcatca
    65581 tcgaacagtt ttttcagcac gtcgttcgct ttgtaggtca gctctttcat acgcgccagg
    65641 ttctctttgc tcacccagcc aaaggtttcg cagtaagatt cgttgaccat cggatcgtgc
    65701 atggcatcgt ttttcaggaa cagatcgaac agcggcgggt tcagctcaat accttcttcg
    65761 attccaagac gtttcactag agagccagca gcacggttac gcacgacaca ctcaaccggc
    65821 accatatcca gctttttcac cagacattcg gtatcggaga gcagacgctc catttgagtc
    65881 gggatacccg cttcagccag tttgctcata atgaagtagt tgaacttgtt gttcaccata
    65941 cctttgcgat caaactgctc aatgcgcgcg ccatcccctg ctgacgtatc attgcggaat
    66001 tcgagcacca acaggtccgg gttttccgtg ctgtatacgg ttttcgcttt accacgatac
    66061 aactcagctt gcttttgcat ctttatcact cctgggtgtg aattaacgtt ttaaaatctt
    66121 ttgctgtctg gtgtgccgga tgttttgtcg gatgcggcgt gaacgcctta tccgaactac
    66181 gtccgacaaa caaatttcgt gcgaattacc gctaaaatcg cgttttcctg ccgacgcaca
    66241 cgtttgcgta tcatatcaga aaaaagggcc ggatgattcc agccctgtat ttttacttgc
    66301 taaacgcagc ctggaagaca gctaccagcg cgtcgttctg actctgagtc agagtatgac
    66361 ctttcggatc gatgaactgt aggctgctgc ggttatctaa atcgccaacc tgcagtttat
    66421 agtcaccgga tgccaggcct ggatcgctcg cgcccagttc ctgccagtcg ctgtcagaca
    66481 gcggcttata agttacggcc atgttgccct gcgaacgggt gctgtcggtc actttcatgc
    66541 ccactttttc cagcgccgct ggcagacgtt gccaaaccac attgaacggc ccgcgtacga
    66601 ccagcattgg taaaccggtg tcatcagctg cactttgtac gtccatagtg gtggaggcac
    66661 gattttgcgc agcgttcgcg gcgtcagtgg cagatttatc cagaccggcg gaaataacgt
    66721 tcatcatctc cgtgctgtaa cgctgcatgg aagccgcgtc tgcaaccggt ttgcccgcct
    66781 gttccaggtt cagcagttta accgtaaccg cctgctgata accctgcggc ttaacagaga
    66841 tttgataacg accacgatac tgctcgtctt cgtccagacg gttccattgt acccaatcgg
    66901 tggtcagtgt ctgaccagca tcatcacgtt gggtgatggt gtagtttttc gcctgcagca
    66961 cgctaaccac ctgcggccac agagtattgc cacgaccatt ttccaccagc aatgaagcgg
    67021 tatcgcccgt gaactgggta cgcgcgccag aaaccagtgc caacggctgg gctggtggac
    67081 gaatgtccag cgccttaccg acagcaccac taccgttggt caccgggatt gcataatcac
    67141 cggaggtcac cggcaaaatc attccagccg gggcatgaag ctccgcaagc ggtgccgctt
    67201 ccaggtaggc ttcatcacca ctgacctgac gcttatagcg tgagtcagaa ctacaggcag
    67261 cgagtaataa aacaagcgaa acacccgcaa cctttgccag gcgcgacttt tgaacagagt
    67321 aagccatcaa atctccctaa actttacagc aaaccggcat gcttaagcgc cgctctgacc
    67381 gtctcacgac cactgtcggt gattggtgtc attggcaggc gcagcgtatc ggtcgccaca
    67441 agacccagtt ccttacatgc ccatttcacc gggattggat tgggttcgac aaatagtttg
    67501 ttgtgtaatg gcatcagacg ctgattaata acgcgtgcct cggcaaaatg cccttctgct
    67561 gccagtttgc acatctgggc catatcacgc gctgcgacgt tagccgtaac ggaaataact
    67621 ccatgaccgc ccaattgcat gaagtccagc gcgctcgcat catcgccgct cagcagaaca
    67681 aaatcatctg aaaccagctc tttgatctgg tttacacgcg ttaagttccc tgttgcctct
    67741 ttgattccga taatattttt tactttcgcc agacggccca ccgtttccgg gagcagatcg
    67801 cagccagtac gggacggcac attatacaga atttgcggca ggtcagtatg ctcagcgatg
    67861 gctttgaaat gctgatacaa accttcttgc gacggacgat tgtagtaagg ggttaccgtc
    67921 aggcagccga cgataccact gtcattgaag cgctgcgtca ggctaatggc ttccgcagta
    67981 gcgttagcgc cggtcccggc aattaccgga atgcgcccat cagccagatc cagcgtcatc
    68041 atcaccacat cagcatgttc gtcatgattt aaggtagcgg actcgccagt ggtgccaaca
    68101 gaaacgatcg ccgaagtacc gctggcgaca tgataatcaa tcagtttttt caagctagcc
    68161 cgacagacat tacctttttc atccatcgga gtaacaatcg cgacaatact tcccgtgaac
    68221 atgggccatc ctctgtgcaa acaagtgtct caatggtacg tttggtatgg cattaaaagc
    68281 aagcagacag aaccgttctg attgttgtat gcatgatttt tttatgcttt ccttaagaac
    68341 aactcacccc ttaaaggaat aaccagtttg acactgtcat cgcaacatta tctggtgatc
    68401 actgcgttgg gtgccgatcg ccctggaatt gtgaacacca tcacccgtca tgtcagtagt
    68461 tgcggctgta atattgaaga cagtcgcctg gcgatgctgg gagaagagtt cacgtttatc
    68521 atgctgcttt ccggttcatg gaatgccatc actctgattg aatcaacgtt accgttgaaa
    68581 ggtgccgaac tggatctttt aatcgtgatg aagcgcacga cggcgcgtcc gcgtccgcca
    68641 atgccagcat ctgtctgggt tcaggtcgat gttgcagact ccccgcattt aattgaacgc
    68701 ttcacagcac ttttcgacgc gcatcatatg aacattgcgg agctggtgtc gcgcacacaa
    68761 cctgctgaaa atgaacgggc tgcgcagttg catattcaga taaccgccca cagccccgca
    68821 tctgcggacg cagcaaatat tgagcaagcg ttcaaagccc tatgtacaga actcaatgca
    68881 caaggcagta ttaacgtcgt caattattcc caacatgatg aacaggatgg agttaagtaa
    68941 tgaatccact gaaagccggt gatatcgcac cgaaatttag cttgccggat caagacggag
    69001 aacaagttaa tttgaccgac ttccagggac agcgtgttct ggtttatttc tacccgaaag
    69061 ccatgacccc cggctgtacc gtacaggcct gcggcttacg cgataacatg gatgagttga
    69121 aaaaagcggg cgttgatgtg ctgggtatca gcaccgataa acccgaaaaa ctctcccgtt
    69181 ttgcggaaaa agagctgctt aactttacgc tcctgtctga tgaggaccac caggtgtgcg
    69241 aacaattcgg cgtctggggt gaaaagtcct tcatgggcaa aacctacgat ggtattcatc
    69301 gcatcagctt cctgattgac gctgatggca aaatcgaaca tgtctttgac gatttcaaaa
    69361 ccagcaatca ccacgacgtt gtgctgaact ggctgaaaga acacgcctga ttactttgct
    69421 ccattccgtg ctggctgcgc ttgcggccag catacctcac ttctcgtgat caagatcaca
    69481 ttctcgcttt cccctgcgac acgggtgtcg aatccatttt ttgctgaacg ttaatgacca
    69541 tcatttttgt accgttcaga atccagttaa tacataactt attgaataga ttgagttaat
    69601 cagaatggca tcctttatac aatatgaaat gcaatgtttc atatcatttt caaggagccg
    69661 acatgaaccg ctttgtggtg gccgaaccac tgtggtgtac aggatgtaat acctgtctcg
    69721 ctgcctgttc ggacgtgcat aaaacgcaag gtttacagca acacccgcgc ctggccctgg
    69781 cgaagacgtc aacaatcact gcccctgtcg tgtgtcatca ctgtgaggaa gccccttgcc
    69841 tgcaggtctg cccggtcaat gccatctctc agagggatga tgcgatccaa ctcaacgaaa
    69901 gcctctgtat tggctgcaag ctttgcgccg tggtctgccc atttggcgca atcagcgctt
    69961 caggaagccg tccggtgaat gcccatgcgc aatatgtttt tcaggctgaa ggctcactca
    70021 aagacggcga agaaaacgtg ccgccccaac atgcgttgct gcgctgggaa ccgggtgtcc
    70081 agaccgtcgc ggtgaaatgc gacctgtgtg atttcttgcc agaaggtccg gcctgcgttc
    70141 gcgcttgccc gaatcaggcg ttacggctga tcaccgacga tagcctgcaa cgtcagatga
    70201 aagaaaaaca gcgccttgcc gcaagctggt ttgccaatgg cggggagtct cccctttccc
    70261 tcactcagga gcaacactaa tggatgccct gcaattatta acctggtcgc tgatcctcta
    70321 tctgtttgcc agtctggctt cgctgttttt actcggtctg gacagactgg ctattaagct
    70381 ttccggcatc acatcgctgg tgggcggcgt gattggcatc atcagcggaa ttacgcaatt
    70441 acatgctggt gtaactttag tcgcccattt tgccacccct tttgactttg ccgatttaac
    70501 cctgcgaatg gatagcctct cggcatttat ggtgctagtt atctccttgc tggtggtggt
    70561 ttgttcgctc tattcattga cttatatgcg cgaatacgag ggcaaaggcg cggcggcgat
    70621 gggcttcttt atgaatcttt tcatcgcatc gatggttgcc ctgctggtga tggacaacgc
    70681 tttttggttc atcgtgctgt ttgaaatgat gtcgctgtct tcctggtttc tggtcattgc
    70741 caggcaggat aaaacgtcga tcaacgctgg catgctctac ttttttatcg cccacgccgg
    70801 atcggtgctg attatgatcg ccttcttgct gatggggcgc gaaagcggca gcctcgattt
    70861 tgccagtttc gcacgctttc actttctccg gggctggcgt cggcggtgtt tctgctggcc
    70921 tttttcggtt ttggcgcgaa agccgggatg atgccgttgc acagctggtt gccgcgcgct
    70981 caccctgccg caccatcgca cgcttcggcg ttgatgtctg gcgtaatggt caaaatcggt
    71041 attttcggca tcctgaaagt agcgatggat ctgctggcgc aaacgggttt gcctctgtgg
    71101 tggggcattc tggtgatggc gatcggcgca atctccgcgc tcctgggcgt gctatatgcg
    71161 ctggcggaac aggatatcaa acggctgctg gcctggagta ccgtcgaaaa cgtcggcatt
    71221 attttgctgg cagtcggtgt ggcgatggac ggtctgtcac tgcacgaccc gctgctcacc
    71281 gtggttggac tgctcggcgc actgtttcat ctgctcaacc atgcgctgtt caaagggctg
    71341 ctatttctcg gcgcggacgc gattatttcg cgtttgcata cccacgacat ggaaaaaatg
    71401 ggggcactgg cgaaacggat gccgtggaca gccgcagcat gcctgattgg ttgcctcgcg
    71461 atatcagcca ttcctccgct gaatggtttt atcagcgaat ggtacacctg gcagtcgctg
    71521 ttctcactaa gtcgtgtgga agccgtagcg ctacaacttg cgggtcctat tgctatggta
    71581 atgctggcag tcactggtgg gctggcagta atgtgcttcg tcaaaatgta cggtattact
    71641 ttctgtggtg cgccgcgcag tacacacgct gaagaggcac aggaagtgcc aaatacgatg
    71701 atcgtcgcca tgctactgct cgcggcactc tgcgtattaa ttgcgcttag tgccagttgg
    71761 ctggcaccga agataatgca tattgcccat gcgtttacca atacccctcc cgtcactgtc
    71821 gccagcggaa tagcacttgt acccggcacg tttcatacac gggtcactcc ttcattactg
    71881 ttgctgttac tactggcgat gcctttgctg ccaggccttt actggctgtg gtgtcgttcg
    71941 cgccgcgcag cgtttcgtcg cacaggagat gcctgggcat gcggctacgg ctgggaaaac
    72001 gcgatggctc cgtcaggcaa tggcgtgatg cagccgctgc gtgtggtctt ctctgcgtta
    72061 tttcgtctac gacaacagct cgaccctacg ctgaggctaa ataaaggtct tgcgcacgtc
    72121 accgccaggg ctcagagcac agaacccttc tgggatgagc gggtgatccg ccccatcgtg
    72181 agcgccaccc aacggctggt caaagaaata cagcatctgc aaagcggcga ctttcgtctc
    72241 tattgcctgt atgtggtcgc cgcactggtt gtgctgctaa tcgctattgc cgtctaagga
    72301 aatcaccatg agacaaactc tttgcgacgg atatctggtc atttttgcgt tagcacaggc
    72361 cgtgattctg ctgatgctaa ccccactttt tacgggtatt tcccggcaga tacgcgcgcg
    72421 tatgcactcc cgccgcgggc cggggatctg gcaggattat cgcgatatcc acaaactgtt
    72481 taaacgccag gaagttgcgc cgacatcttc aggtctgatg ttccgcctga tgccgtgggt
    72541 attaatcagc agcatgctgg tgctggcgat ggccttacca ctatttatta ccgtttcccc
    72601 ttttgcgggc ggcggcgatc tgatcaccct tatctatctt cttgccctgt ttcgtttttt
    72661 ctttgctctt tccgggctgg ataccggaag tccgtttgcg ggagtcggtg ccagtcgcga
    72721 gttgacgctc ggcattctgg tcgaaccaat gcttattctc tcactgctgg tattggcgct
    72781 gatagcaggt tccacgcata tcgagatgat cagcaatacg ctggctatgg gctggaactc
    72841 gccgctaacc accgtactgg cgttactggc ctgtggtttt gcctgcttca ttgagatggg
    72901 aaaaattccc tttgatgttg ctgaagcaga acaggaatta caggaaggcc cgctgaccga
    72961 atattccggt gccgggctgg cgctagcgaa atgggggctg gggctgaaac aggtcgtgat
    73021 ggcagcactg tttgtggccc tgtttctgcc ctttgggcgc gcgcaagaac tttctctcgc
    73081 ctgcctgctg acttcacttg tcgttacgct gctcaaggtt ttgctgattt ttgtactggc
    73141 ctcaatcgca gaaaacacgc tggcacgcgg gcgtttttta ctcattcacc atgtgacctg
    73201 gcttggcttc agccttgctg cgcttgcatg ggtcttttgg ttaaccggtc tgtaaggagc
    73261 actgacggaa tatggaaaat cttgctctga cgacgttaat gctgcctttt atcggcgcac
    73321 tggtcgtttc gttttcgcca caacgtcggg ccgccgaatg gggggttttg ttcgccgcgc
    73381 tgaccacgct gtgcatgttg tcgctgatct ccgcgtttta tcaggccgat aaagttgccg
    73441 tcacgttgac gttggtcaac gtgggggatg tggcattatt tggcctggtc attgatcgcg
    73501 tgagtacgct gattctgttt gtggtggtgt ttctcggttt gctggtcacg atctactcca
    73561 cgggttatct gacggataaa aatcgcgaac acccgcataa cggcacgaat cgttattacg
    73621 catttttgct ggtgtttatc ggcgcgatgg cgggactggt actctcctcg acgctgctcg
    73681 gtcagttgtt gttttttgaa attacgggcg gctgctcctg ggcgttgatc agttattacc
    73741 agagcgataa agcgcagcgt tcagcactaa aagcgttgct tatcacccat atcggttcgt
    73801 tggggttgta tcttgccgcc gccacgctgt ttttgcagac cggaacgttt gtgcttagcg
    73861 cgatgagcga gttacatggc gacgcacgtt atctggttta tggcggcatc ctgtttgccg
    73921 cgtgggggaa atcggcccag ctaccgatgc aagcgtggct accggacgca atggaagcgc
    73981 caacaccgat cagcgcctat ctccacgccg catcgatggt gaaagtgggc gtttacattt
    74041 ttgcccgcgc cattatcgac ggcggcaata tcccgcatgt gattggcggc gttggcatgg
    74101 tcatgacact ggtcaccatt ctttatggct ttctgatgta tttgccacag caggatatga
    74161 agcggttgct ggcctggtcg accatcactc aacttggctg gatgttcttc ggcttgtcgc
    74221 tctccatctt cggctcgcgg ctggcgctgg agggtagcat cgcctacatc gtcaaccacg
    74281 cgttcgctaa aagcctgttt ttccttgtag caggtgcgct gagttacagc tgcggcacgc
    74341 gcttgttgcc gcgtctgcgt ggcgtattgc acaccctgcc gttgccaggc gtgggtttct
    74401 gcgtggcagc gctggcaatt accggtgtgc cgccgttcaa cggcttcttc agtaaattcc
    74461 cgctgtttgc cgccggtttt gcgttgtcag tggagtactg gatcctgctg cccgccatga
    74521 ttctgctgat gattgaatcg gtcgccagtt tcgcctggtt tattcgctgg tttggtcgcg
    74581 tcgtgccggg caaaccgagc gaggccgtcg ccgatgccgc accgctgcca gggtcaatgc
    74641 gcctggtgtt gattgtactg attgtgatgt cgctgatttc cagcgtaatc gccgcgacct
    74701 ggttgcagta aggagatgat gaatgaccgg ttctatgatc gtaaataatc tggcgggact
    74761 gatgatgctg acatcgctgt ttgtgattag cgtcaaaagc tatcgcctgt catgcggatt
    74821 ttacgcctgc cagtcgctgg tgctggtgtc tattttcgcc actctctcgt gcctgttcgc
    74881 cacagagcaa ctgctgatct ggtccgccag cgcctttatc accaaagtgc tgctggtacc
    74941 gttaatcatg acttacgctg cacgaaatat tccccagaac atcccggaaa aagcgttatt
    75001 cggtccggca atgatggcac tgctcgcggc gttaattgtc ctgctttgcg catttgtcgt
    75061 tcagcccgtg aagctaccga tggctaccgg gctgaaaccg gcgctggcgg tagcgttagg
    75121 tcattttctg cttggcctgc tgtgcattgt cagccaacgc aatatcctgc ggcaaatttt
    75181 tggttactgc ctgatggaaa acggctccca tctggtgctg gcgcttcttg cctggcgagc
    75241 accggaactg gtggaaatag gtatcgctac cgacgccatc ttcgccgtca ttgtgatggt
    75301 gttactggca agaaaaatat ggcgtaccca cggcacgctg gacgtgaaca acttgaccgc
    75361 gctgaaggga taatgagatg agttattctg tgatgttcgc tttactcctg ctcacgccgc
    75421 tgcttttttc gctgctctgt tttgcctgcc ggaaacgggg gctctctgcg acttgtacgg
    75481 tgactgtatt acatagctta gggatcacac tgctgctgat tctagcactc tgggtggtcc
    75541 aaactgccgc tgatgcagga gaaatattcg ctgcgggact gtggcttcat attgatggtc
    75601 tgggcggttt gttcctcgcc attcttggtg tgattggctt tctcaccggt gtttactcga
    75661 ttggctacat gcgtcatgaa gtggcacacg gcgagctttc acccgttaca ctgtgcgatt
    75721 actacggttt cttccatctg tttttgttca ccatgctgct ggttgttacc agcaataacc
    75781 tgattgtgat gtgggcggcg atcgaagcca ccaccttaag ctcggcgttt ctggtaggca
    75841 tttacggtca gcgttcatcg ctggaatcag catggaagta catcattatt tgtactgttg
    75901 gtgtcgcttt tggtctgttc ggtaccgtgc tggtatacgc caacgccgcc agcgttatgc
    75961 cgcaggcaga aatggcgata ttctggagcg aggttcttaa gcaatcgtcc ttgcttgacc
    76021 caacattaat gctgttggcc tttgtgtttg tgctaattgg ctttggtacc aaaaccgggc
    76081 tatttcccat gcacgcctgg ctgccggatg ctcacagtga agcgccgagt ccggtcagcg
    76141 ccctgctctc cgccgtattg ctgaactgcg cgctgttggt gctgattcgc tattacatca
    76201 ttatttgcca ggccatcggc agcgatttcc ccaaccggtt gttgctcatc ttcggcatgt
    76261 tgtcggttgc cgtggcggca tttttcattc tggtacagcg ggacattaag cgtctgctgg
    76321 cgtactccag cgtggagaac atggggctgg tcgcggtggc gctaggcatt ggcgggccgc
    76381 tgggaatttt tgccgcgctg ctgcacacct taaaccacag tctggcaaaa acgctgctgt
    76441 tctgcggttc cggcaatgta ctgctcaagt acggcacgcg cgatctcaac gtcgtctgtg
    76501 ggatgctcaa aatcatgcca tttaccgccg tgctgtttgg cggcggtgcg ctggcgctgg
    76561 cgctggcagg gatgccgccc ttcaacattt ttcttagcga atttatgacc gttaccgcag
    76621 gactggcacg taatcacctg ctgattatcg tcctgctgtt attgctgtta acgctggtgc
    76681 tggcgggcct ggtacggatg gctgcgcggg tgttaatggc gaaaccgccg caggccgtta
    76741 accggggtga tctcggctgg ttgaccacct cgccaatggt gattctgctg gtcatgatgc
    76801 tggcgatggg aacgcatatt ccacaacctg tcatcaggat cctggcgggc gcttccacta
    76861 tagtcctctc agggacgcac gacctgcctg cacaacgtag cacctggcat gattttttgc
    76921 cttcaggcac cgcatctgtt tcggagaaac acagtgaacg ttaattcatc gtcaaatcgt
    76981 ggcgaagcga ttctcgccgc cctgaaaacg cagttccccg gcgcggtgct ggatgaagag
    77041 cgacaaacgc ctgaacaggt caccattacg gtgaaaatca atctgctgcc tgacgttgta
    77101 cattatcttt attatcaaca tgatggctgg cttccagtcc tgtttggcaa cgacgagcgg
    77161 acacttaacg gtcattacgc ggtttattat gccctttcaa tggaaggggc cgaaaaatgc
    77221 tggatcgtgg tgaaggcact ggtcgatgcc gacagtcggg agtttccgtc agtcacaccg
    77281 cgcgtccctg ccgcggtctg gggcgagcga gaaattcgcg atatgtacgg gctgattccg
    77341 gttggcctgc cggatcagcg tcgcctggtg ttgcccgatg actggccgga agatatgcat
    77401 ccgctgcgca aagatgcgat ggattatcga ctgcgccctg aaccgacgac agattccgaa
    77461 acgtatgcgt ttatcaatga gggcaacagc gatgcgcggg tgatccctgt cggcccgctg
    77521 catatcacct cctatgaacc gggtcacttc cgcttgtttg tggatggcga gcaaattgtc
    77581 gatgctgatt accgcctgtt ttatgtccat cgcggcatgg agaaactggc agaaacgcgg
    77641 atgggctaca acgaagtgac cttcttatcg gaccgcgtgt gtgggatttg cggttttgcc
    77701 cacagtgtgg cctataccaa ctcggttgaa aatgcactgg ggattgaggt gccgcaacga
    77761 gcacatacta ttcgctcgat tctgctggaa gtcgagcggc tacacagtca tttgctcaac
    77821 cttggcctct cctgccattt tgttggtttt gataccggct ttatgcaatt tttccgcgtg
    77881 cgggaaaagt cgatgacgat ggcggaattg ctgaccgggt cgcgtaaaac ctacggtctg
    77941 aatctgattg gtggtgttcg ccgcgatatt ctcaaagagc aacgtctgca aacgctgaaa
    78001 ctggtgcgcg agatgcgcgc cgacgtgtcg gagctggtag agatgctgct tgctacgccg
    78061 aatatggaac aacgcactca gggcattggc attctcgacc gacaaatcgc ccgtgattat
    78121 agccctgtag ggccgctgat ccgcgcagtg gttttgcccg tgatttgcgc tttgatcacc
    78181 cctacgccga ctacggcaat attccaaaaa cactgtttac ctttaccggc ggcgatgttt
    78241 tctcccgcgt gatggtccgt gtcaaagaga cgtttgattc gctggcaatg ctggaatttg
    78301 ccctcgacaa catgccggat accccactgc tgaccgaagg ctttagctat aaacctcacg
    78361 cattcgcgct gggctttgtt gaagcgccac gcggtgaaga cgtgcactgg agcatgctcg
    78421 gtgataacca aaaattgttc cgctggcgct gtcgtgccgc cacctacgcc aactggccgg
    78481 tgttgcgtta catgctgcgc ggcaataccg tttctgacgc accgctgatt atcggtagcc
    78541 ttgatccctg ctactcctgt accgaccgtg tgacgctggt agatgtgcgc aagcgccagt
    78601 caaaaaccgt gctgtataaa gagatcgaac gctacggcat tgatcgtaac cgttcgccgc
    78661 tgaagtaagg acagaagatg ctgaagttac tgaaaactat tatgcgcgcc ggaaccgcga
    78721 cggtgaaata tcccttcgcg ccactggagg tcagcccagg ctttcgcggg aaaccggacc
    78781 tgatgcccag ccaatgtatt gcctgcggtg cctgcgcctg tgcttgtccg gcaaatgcgc
    78841 tgactatcca gaccgacgac cagcaaaatt cgcgcacctg gcagctctat ctggggcgtt
    78901 gtatttactg cggacgttgt gaagaagtgt gcccgaccag agccatccag cttaccaata
    78961 actttgaact gaccgtcacc cataaagccg atctctatac ccacgcgacg ttccatctac
    79021 aacgttgcag ccgttgcgaa cgcccgtttg ccccgcaaaa aaccgtcgca ctggctgctg
    79081 aattgttggc acagcaacaa aatgctccac aaaaccgcga aatgttatgg gcgcaagcga
    79141 gcgtctgccc ggagtgcaaa caacgcgcaa cgctgatcaa cgacgataca gatgtaccgc
    79201 tggtggctaa ggagcagcta tgagtccagt gcttacgcaa tatgtcagcc agcccatcac
    79261 gctggacgag caaacgcaaa agatgaagcg gcatttgcta caggatatcc gtcgctcggc
    79321 ttacgtttat cgcatcgatt gcggcggctg caacgcctgt gaaatcgaaa tttttgctgc
    79381 catcaccccc gtattcgacg cagaacgttt tggcattaag gttgtttcat caccgcgtca
    79441 cgccgatatt ttgttattta ctggcgcagt cacccgggcg atgcgtatgc ctgcacttcg
    79501 ggcgtatgag tctgcccccg atcataaaat ctgtgtttcc tacggcgcgt gcggtgtcgg
    79561 cggcggtatt ttccacgatc tctacagcgt ctggggcggc agcgacacca ttgtccccat
    79621 tgatgtttgg atccccggct gcccgccaac accggccgcc accattcacg gtttcgccgt
    79681 ggcgctcggt ttgctgcaac agaagattca cgcggtggat tatcgcgatc ccaccggggt
    79741 gactatgcaa ccgttgtggc cgcagatccc gccatcacag cgtatcgcca ttgagcgaga
    79801 agcgcggcgg ctggcgggct atcgtcaggg gcgagaaatt tgcgatcggc tcctgcgcca
    79861 tttaagcgac gatcctacag gaaatcgggt taacacctgg ttgcgcgatg ccgacgatcc
    79921 acgtctcaat agtatcgttc agcaactctt tcgcgtactc cgggggttac atgactgaag
    79981 agtgcgggga aattgttttc tggacgctgc gaaaaaagtt tgtcgccagt agcgacgaga
    80041 tgccggaaca cagctctcag gtaatgtatt actcgctggc tatcggtcat cacgttggcg
    80101 tgattgattg tctgaatgtc gccttccgct gcccactgac ggaatacgaa gattggcttg
    80161 cactggtcga agaggagcaa gcccgacgta agatgctggg ggtgatgact tttggtgaga
    80221 ttgttattga cgccagccac accgccctgt tgacccgggc attcgcgcca ctggcagatg
    80281 acgcgacgtc tgtgtggcaa gcgcgtagca ttcaattcat tcatctgttg gatgaaattg
    80341 tgctggaacc ggctatctat ctgatggcca gaaaaattgc gtgagaagga tttctcatta
    80401 ataaggactg ttgatggcta tgtcagacga agcgatgttt gccccgccgc aaggaataac
    80461 aattgaagcg gtaaacggaa tgctcgcgga gcggttagcg cagaaacacg gcaaggcgtc
    80521 tttattacgc gccttcatcc cgctgccgcc gccgttcagc ccggtacaac ttattgaact
    80581 gcatgttctc aaaagcaact tctattaccg ctaccatgat gatggcagcg atgtgacggc
    80641 aacaacagag tatcagggcg agatggtcga ttattcgcgt tacgccgtcc ttctcggcag
    80701 tagtggaatg gcggagctac gctttattcg tacccacggc agccgtttta ctccccagga
    80761 ttgcacactg tttaactggc tggcacgcat tatcaccccg gttctgcaat catggctcaa
    80821 tgatgaagca cagcaggtgg cgctgcgttt gctggagaaa gatcgcgatc atcatcgggt
    80881 actggtggat atcactaatg cagtgctgtc acatcttgat ctcgacgatc tgatcgctga
    80941 cgtcgctcgt gagatccatc attttttcgg tctggcttca gtcagtatgg tactgggcga
    81001 tcatcgaaag aacgagaagt tcagcctgtg gtgcagcgat ctttctgcct cacattgtgc
    81061 gtgtctgcca cacaatatgc ctggcgacag tgtattgctg acacaaacgc tacaaacccg
    81121 acaaccgacc ttgacgcacc gtgcagatga tctgtttctc tggcaacgcg acccgttatt
    81181 actcttactt gcatctaacg gctgcgaatc tgcgctcctt ataccgctta cctttggcaa
    81241 ccatacaccg ggtgcattgt tgctggcgca tacctcttcc actctcttta gtgaggaaaa
    81301 ctgccagcta ctacaacaca tagccgatcg catcgctatt gccgttggca atgccgatgc
    81361 ctgccgtaga atgaccgatt tgcaggaaag tttgcagcaa gaaaaccacc agcttagcga
    81421 gcagctcctt tcgaatctgg gcgtcggtga cattatctat caaagccagg caatggaaga
    81481 cctgctccag caggtagata ttgtggcgaa gagcgacagt acggtgttga tttgcggtga
    81541 aaccggaacc ggcaaagagg tgatcgccag agcgatccat caacttagcc cgcgacacga
    81601 caagccgctg gtcaaaatca actgcgctgc catccccgcc agtcttctgg aaagtgagtt
    81661 attcggtcat gacaaagggg cgtttactgg tgcgattaat acccatcgtg gtcgttttga
    81721 aattgccgat ggcggcacgt tgtttctcga tgaaattggc gatctgccgt tagaacttca
    81781 gcctaaactg ctgcgcgtat tgcaggaacg ggagattgag cgtctcggcg ggagtagaac
    81841 gatcccggta aatgtcagag tcattgccgc caccaaccgt gatttgtggc aaatggttga
    81901 agatcgccag tttcgcagcg atctctttta tcgcctgaat gtcttcccac tggaattgcc
    81961 tccgctgcgc gaccgtccgg aagatatccc tcttttagca aagcatttca cgcaaaaaat
    82021 ggcgcgccat atgaatcgtt caattgacgc catcccgacc gaggcgctac gccagttgat
    82081 gtcgtgggac tggccgggca acgtgcgcga gctggaaaac gtgattgagc gagcggtact
    82141 gctgactcgc ggtaacagtc tgaatttaca tcttaatgtc cgacaaagcc gtttactgcc
    82201 gacgctaaat gaagattcag cgcttcgcag ttcaatggcg tagttgctgc acccgacgac
    82261 gccagagaat gacgaagaag aacgtcagcg cattgttcag gtattgcgag aaaccaatgg
    82321 cattgttgcc gggccccgtg gcgcggcgac acgattaggg atgaagcgca ccacgctgct
    82381 gtcacgaatg cagcgtctgg gaatctcggt tcgcgaggtg ttgtaatctg cttttgcagg
    82441 agtatgcatg agaaacaaac tctctttcga cttgcagttg agcgccagaa aagcggcaat
    82501 cgctgaacgg attgccgccc ataaaattgc ccgcagtaaa gtgtcggtct ttttaatggc
    82561 gatgtccgct ggcgtgttta tggcgatgtc cgctggcgtg tttatggcga tcggatttac
    82621 tttttacctt tccgttatcg ccgatgcccc gtcttcatag gcattaaccc atctggtggg
    82681 cggcctttgc tttacacttg gctttatttt gctggcggtt tgcggcacca gcctgttcac
    82741 ctcgtcggta atgacggtaa tggcaaaaag tcggggcgtt attagttggc gaacttggct
    82801 gattaacgca cttctggtgg cctgcggtaa tctggcaggt attgcctgtt tcagtttgtt
    82861 aatctggttt tccgggctgg tgatgagtga aaacgcgatg tggggagtcg cgattttaca
    82921 ctgcgccgag ggcaaaatgc atcatacatt tactgaatct gtcagcctcg gcattatgtg
    82981 caatctgatg gtttgcctgg cgctgtggat gagttattgc ggacgttcgt tatgcgacaa
    83041 aatcgtcgcc atgattttgt ccatcaccct gtttgtcgcc agtggctttg agcactgtat
    83101 cgccaatttg tttgtgattc cgttcgccat tgccattcgc catttcgccc ctacctcctt
    83161 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtca gccattttat
    83221 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
    83281 tatttgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
    83341 gttcgtcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc
    83401 gtcgggctga agattattct tgcgcgattt gcccatcggg ccaggcgtgg atcacggctt
    83461 tgatcagcgt cgccaatgga atggcgaaga atacgcccca gaatccccac aaaccaccga
    83521 agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg gagaacaaca
    83581 ccggtactag caggttgccg tccagcgcct gaataatcag atacaccgcg aagcagctcc
    83641 agaattcagt acctgcgcca aactggaata gcgccacgcc aaccaccgga atggtcacca
    83701 caaatgcgcc aatgtacgga ataagaaccg agaagccgac cagcactgcc agcagcagcg
    83761 aatagttcag cccgaagagc aagaacccca gccaggtggc gatccccacc acgatcatct
    83821 ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc cacacctgtc
    83881 ctgccagccc acggttacgc ggcagcaccc ggcgaacggc gttcagcatc tgctctttgt
    83941 ctttcagcag gaagaagacc atcaatggca ccagcaccag atagacggct atggtcagca
    84001 aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc agcatccgac
    84061 tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaacacc ggatagcggc
    84121 gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata tcgcggatta
    84181 agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt agcaatatac
    84241 cgacaaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca atagattgca
    84301 ggcgcacggt tggccattcc agcaaataag ccagcacaat agccaccagc aacggagcaa
    84361 gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc agcaaggcaa
    84421 tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg agcataaaga
    84481 ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat gtcacaaaaa
    84541 agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg ggctaatctt
    84601 cgccgttaca ctcaaaggcg gcgaggtggg aacgatattt cacagtatcg gtcaaatgac
    84661 tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa cctggttgca
    84721 accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga cagcgcagac
    84781 accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca ggaaatgcag
    84841 atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa tgacccgctg
    84901 ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa ttcggttaag
    84961 acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc tttctttggc
    85021 ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag tcaactggct
    85081 tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg agcgatggaa
    85141 gatcaacaac gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat tttactggcg
    85201 atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg aacgcgccag
    85261 gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg tattcaggtg
    85321 ctgcaacgct cgggattcga tccacaggcg atgccgacct tcctggaaaa attgctcgat
    85381 caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt gccggaaagt
    85441 cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt gcagtcgtcg
    85501 gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg acgtaaccag
    85561 ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca acgagcggcg
    85621 caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc gcgtaaaacg
    85681 ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct ggctaccgat
    85741 atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa tgcccgcgat
    85801 ttacgcacca atccggtgtt gcagctcaac ctggcgaacg cttatctgca aggcggtcaa
    85861 ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga tgacagcaac
    85921 ggctgggatt tgctggcaca ggcggaagcc gcgctaaata accgcgatca ggagctggct
    85981 gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc gctgttgagt
    86041 agcgccagtt cgcaggtgaa attaggcagc ctgcaacaag cgcgttacga tgcgcgcatc
    86101 gaccagttgc gccagctgca gaaacgcttt aagccttata ccaagatgta attcgcatta
    86161 aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt tcaaagagcc
    86221 gggaaacgct gaacctgctg aaagaaaacg gcgtggagcc agaagtggtg ctctaccttg
    86281 agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg atgaatagcg
    86341 cccgtgaatt gatgcgccag aaagaggatc tttataaaga actaaacctg gctgacagtt
    86401 cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg atggagcgcc
    86461 cgattatggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag gtactggaaa
    86521 tcgtgggtta agatgtgctc catcgctagt tgaagcacat tgccggatgc gacgcttgcc
    86581 acgtcttatc cggcctacga atactatgga ttaacttcgc gtaggtcagg cgttcgcgcc
    86641 gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa cggaatggtc
    86701 agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat aaatagcgtg
    86761 cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg cagttcaaaa
    86821 ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga aagtggctgc
    86881 aatttgtaga tctgccccca gtcgagacgc gacgcgagat ccggtaatcc cagattcaac
    86941 tgccgcggtg gacgatcgcc ggtgatcaac aaccgtgttt tgcccgattc cagaattcga
    87001 ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca ctcaatgttg
    87061 tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac aaaccaggtg
    87121 cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc cgcgcaagcc
    87181 gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat gtaaccgcta
    87241 tgttcctgac gcagcacgtt ttgcagcgcg gccagtaaag aggagttatc ccccggccag
    87301 aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg tgccggtgtg
    87361 ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac agaatcccat
    87421 gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg ttatatccgc
    87481 gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctgatcagtt tgaaaatcaa
    87541 acttaagccg ataccgacga tggtcgccag cgccatacct tttaactccg ccgcgccgat
    87601 gtttaccttc gcgccactga cgccgatgat caaaatcacg gaagtcagga tcaggttctg
    87661 tgctttgttg taatccactt tcgattcgat caaaacacga ataccggaag caccaatgac
    87721 accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct ggatagcggc
    87781 agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccgc cgataaccca
    87841 ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag tggtatttgg
    87901 cgtagagcca aagaagccgg agatcacggt cgacaggccg ttagcaaaca tcgaacggtg
    87961 cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta ccaggtgccc
    88021 tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg caaaccactc
    88081 gaagcgcggc gtatagaggg ttggcagtgc aaaccagtga gcattaataa tcggcgtggt
    88141 atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa ttaaaatcgg
    88201 gataattgcc aggaaaccac gaaacagcac ggaacctaaa accgtgaccg ccagggtggt
    88261 aatagagatg atgatggttt tggagtttgg cgtttgccct tcagccggga gtaaacccgc
    88321 cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg cgcccattgc
    88381 cgcaggtgga aacagcacgt ccaaccagcc ggtccccgct ttcttcacga taaaagaaac
    88441 caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat accctaacgg
    88501 caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag ccggaatttt
    88561 ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca gtacagtcgc
    88621 cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg cgaacaaatg
    88681 ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc cgatagcacg
    88741 gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca aaaaaaagcc
    88801 gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag attttgtcac
    88861 cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg tcaatcgatg
    88921 cagtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct tctggcgcag
    88981 cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg tcgatggtcg
    89041 cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata cgctcatcga
    89101 tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca ttacggtaca
    89161 taccgacaac gctgatgcgc gcgctcggaa cgttttccag cacaccgtcc atcataccaa
    89221 gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg tcgatttcta
    89281 ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg gtcgcttcgt
    89341 aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg ctgatatctt
    89401 gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc acgatcttca
    89461 tactctttct cctttgaggg gcagccacaa aaaaatcgac ggattatacc tcctttcttc
    89521 agggcggcaa tattattttc gttgacttta gtcaaaatga taacagtttg agataaagtt
    89581 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa cctttgccct
    89641 ggcacaagtc ttctttcgcc gcgcgcccgg ggaaaagacg tgcaaaaagg ttgtgtaaaa
    89701 cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
    89761 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
    89821 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
    89881 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
    89941 caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
    90001 ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
    90061 aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga
    90121 aaactggatg ttgataccgc ttcagcggtg atcagcggca tcgcggaagg ttgtctgcaa
    90181 tcgggctgtt ctctggtggg tggcgaaacg gcagaaatgc cggggatgta tcacggtgaa
    90241 gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat catcgacggc
    90301 tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac
    90361 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
    90421 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
    90481 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
    90541 ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat tgatgaatct
    90601 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt tgagcgccat
    90661 gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa
    90721 gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc
    90781 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
    90841 tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta
    90901 aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
    90961 gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt cgtgaagcct
    91021 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
    91081 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
    91141 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
    91201 atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
    91261 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
    91321 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
    91381 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg
    91441 gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc gctggcgttt
    91501 ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc tggcatagtt
    91561 ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag
    91621 taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt
    91681 tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc
    91741 gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac
    91801 tgaagcgacg catcattatt agcgaagaac aaggctccaa cgctcattcc cgccatttac
    91861 tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg
    91921 agctattgct ggaaatggcg cgcaaccaga tcttcctgat taatgaacgc cagctctccg
    91981 tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc
    92041 cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc
    92101 tggcggtgga aattatccgt agcgatagca tccgttacgc gctgctggag atcccatcag
    92161 ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc aagccgatga
    92221 ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt
    92281 atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc
    92341 atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg
    92401 ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac
    92461 gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt
    92521 ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc
    92581 cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc
    92641 gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg ctggaactgc
    92701 tgcgtcaggc ttcgttcgac ccaagcgtac tggcgattaa aattaacatt taccgcgtgg
    92761 cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt aagaaagtca
    92821 ctgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc
    92881 gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca
    92941 aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga
    93001 ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag
    93061 atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc
    93121 cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa
    93181 tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc
    93241 taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg
    93301 taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta
    93361 gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata
    93421 tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca
    93481 atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg
    93541 tgctggacat catcgacata ttgttcagcg atacggtcaa agcacgttat atcgataaag
    93601 aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga
    93661 tttacgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa
    93721 tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc
    93781 atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat
    93841 ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg
    93901 aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata
    93961 gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa
    94021 aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt
    94081 atgggcgtgg aacataccca accggaaaaa ggtcgcaaac tggttattga tattggcggc
    94141 ggatctacgg aactggtgat tggtgaaaat ttcgaaccta ttctcgttga aagccgccgg
    94201 atgggttgtg tcagctttgc ccagctttat tttcctggcg gggtcatcaa taaagagaat
    94261 tttcagcgcg ctcgcatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt
    94321 attcagggct ggaacgttgc aatgggcgct tccggtacca taaaagccgc ccatgaagtg
    94381 ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta
    94441 aaagaaattt tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag
    94501 cggaaaacag tcttcgttcc tggactggcg attttatgtg gtgtgtttga tgctttagcc
    94561 atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta tgaaatggaa
    94621 ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat
    94681 cacatagaca gcgaacaggc ccgacgagtg ctggatacca ctatgcaaat gtacgaacag
    94741 tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc
    94801 gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct
    94861 tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg
    94921 acactggtgc gctatcaccg taaagcgatt aagctcgacg atctgccgcg ctttaccttg
    94981 tttaagaaga aacagttcct gccactgata cagctattgc gccttggcgt attactaaac
    95041 aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac
    95101 tggacactgc gtttcccgca tgactggttt agtcagaatg cgctggtact gcttgatctg
    95161 gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa
    95221 agtaccccag aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt
    95281 attcaggcac tttcgcgaat gggttcgatt tcattcagcg tatcaattaa cggttgcggc
    95341 ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc
    95401 tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa
    95461 atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat aaagctgccg
    95521 tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg
    95581 ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc
    95641 gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct
    95701 tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc
    95761 gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc
    95821 ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg
    95881 tgcgcgaccg gcaagaagct accggggccg atcagttcat cattctcacc tttcatgcgc
    95941 agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa
    96001 aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat
    96061 tcgcgttgca aatacattgc cccgcaacgc tgcatatttt ccggggcgtt ggtcacgatg
    96121 gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag
    96181 cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa
    96241 cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc
    96301 agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag
    96361 ggtgacagcc agtgagaaag tttttgcttg t
//
LOCUS       DADLEE010000002        95092 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000002 DADLEE010000000
VERSION     DADLEE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.002"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="JQH51_000098"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="JQH51_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY6074598.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="JQH51_000099"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="JQH51_000099"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY6074599.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="JQH51_000100"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="JQH51_000100"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY6074600.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="JQH51_000101"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="JQH51_000101"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY6074601.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="JQH51_000102"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="JQH51_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY6074602.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="JQH51_000103"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="JQH51_000103"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY6074603.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="JQH51_000104"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="JQH51_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY6074604.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="JQH51_000105"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="JQH51_000105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY6074605.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="JQH51_000106"
     CDS             complement(8942..9223)
                     /locus_tag="JQH51_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074606.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="JQH51_000107"
     CDS             complement(9237..10898)
                     /locus_tag="JQH51_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY6074607.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="JQH51_000108"
     CDS             complement(10882..11208)
                     /locus_tag="JQH51_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY6074608.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="JQH51_000109"
     CDS             complement(11264..11446)
                     /locus_tag="JQH51_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074609.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="JQH51_000110"
     CDS             complement(11430..11870)
                     /locus_tag="JQH51_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6074610.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="JQH51_000111"
     CDS             complement(11870..12166)
                     /locus_tag="JQH51_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY6074611.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="JQH51_000112"
     CDS             complement(12163..12501)
                     /locus_tag="JQH51_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY6074612.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="JQH51_000113"
     CDS             complement(12498..13709)
                     /locus_tag="JQH51_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY6074613.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="JQH51_000114"
     CDS             complement(13711..14283)
                     /locus_tag="JQH51_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY6074614.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="JQH51_000115"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="JQH51_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15784..15945)
                     /locus_tag="JQH51_000116"
     CDS             complement(15784..15945)
                     /locus_tag="JQH51_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY6074615.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15955..16260)
                     /locus_tag="JQH51_000117"
     CDS             complement(15955..16260)
                     /locus_tag="JQH51_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074616.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16257..16535)
                     /locus_tag="JQH51_000118"
     CDS             complement(16257..16535)
                     /locus_tag="JQH51_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074617.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16824..18572)
                     /locus_tag="JQH51_000119"
     CDS             complement(16824..18572)
                     /locus_tag="JQH51_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY6074618.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18569..18868)
                     /locus_tag="JQH51_000120"
     CDS             complement(18569..18868)
                     /locus_tag="JQH51_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY6074619.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18874..19107)
                     /locus_tag="JQH51_000121"
     CDS             complement(18874..19107)
                     /locus_tag="JQH51_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074620.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19109..19329)
                     /locus_tag="JQH51_000122"
                     /pseudo
     CDS             complement(19109..19329)
                     /locus_tag="JQH51_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19322..19543)
                     /locus_tag="JQH51_000123"
     CDS             complement(19322..19543)
                     /locus_tag="JQH51_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074621.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19533..19733)
                     /locus_tag="JQH51_000124"
     CDS             complement(19533..19733)
                     /locus_tag="JQH51_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY6074622.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19863..20042
                     /locus_tag="JQH51_000125"
     CDS             19863..20042
                     /locus_tag="JQH51_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074623.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20167..20496
                     /locus_tag="JQH51_000126"
     CDS             20167..20496
                     /locus_tag="JQH51_000126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074624.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20546..20734)
                     /locus_tag="JQH51_000127"
     CDS             complement(20546..20734)
                     /locus_tag="JQH51_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY6074625.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21109..22338)
                     /locus_tag="JQH51_000128"
     CDS             complement(21109..22338)
                     /locus_tag="JQH51_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6074626.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22587..23708
                     /locus_tag="JQH51_000129"
     CDS             22587..23708
                     /locus_tag="JQH51_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY6074627.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="JQH51_000130"
     CDS             complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="JQH51_000130"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY6074628.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25233..26369
                     /gene="potA"
                     /locus_tag="JQH51_000131"
     CDS             25233..26369
                     /gene="potA"
                     /locus_tag="JQH51_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY6074629.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26353..27210
                     /gene="potB"
                     /locus_tag="JQH51_000132"
     CDS             26353..27210
                     /gene="potB"
                     /locus_tag="JQH51_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY6074630.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27207..28001
                     /gene="potC"
                     /locus_tag="JQH51_000133"
     CDS             27207..28001
                     /gene="potC"
                     /locus_tag="JQH51_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY6074631.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27998..29044
                     /gene="potD"
                     /locus_tag="JQH51_000134"
     CDS             27998..29044
                     /gene="potD"
                     /locus_tag="JQH51_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY6074632.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="JQH51_000135"
     CDS             complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="JQH51_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY6074633.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="JQH51_000136"
     CDS             complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="JQH51_000136"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY6074634.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="JQH51_000137"
     CDS             complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="JQH51_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY6074635.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="JQH51_000138"
     CDS             complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="JQH51_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY6074636.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="JQH51_000139"
     CDS             complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="JQH51_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY6074637.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34376..35449
                     /locus_tag="JQH51_000140"
     CDS             34376..35449
                     /locus_tag="JQH51_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY6074638.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35577..39023
                     /gene="mfd"
                     /locus_tag="JQH51_000141"
     CDS             35577..39023
                     /gene="mfd"
                     /locus_tag="JQH51_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY6074639.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39166..40128
                     /gene="ldtC"
                     /locus_tag="JQH51_000142"
     CDS             39166..40128
                     /gene="ldtC"
                     /locus_tag="JQH51_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY6074640.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40211..40423)
                     /locus_tag="JQH51_000143"
     CDS             complement(40211..40423)
                     /locus_tag="JQH51_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY6074641.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40708..41340
                     /gene="comR"
                     /locus_tag="JQH51_000144"
     CDS             40708..41340
                     /gene="comR"
                     /locus_tag="JQH51_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY6074642.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41402..41941)
                     /locus_tag="JQH51_000145"
     CDS             complement(41402..41941)
                     /locus_tag="JQH51_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY6074643.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42168..43472)
                     /locus_tag="JQH51_000146"
     CDS             complement(42168..43472)
                     /locus_tag="JQH51_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY6074644.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43880..44422)
                     /locus_tag="JQH51_000147"
     CDS             complement(43880..44422)
                     /locus_tag="JQH51_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6074645.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="JQH51_000148"
     CDS             complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="JQH51_000148"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY6074646.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="JQH51_000149"
     CDS             complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="JQH51_000149"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY6074647.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="JQH51_000150"
     CDS             complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="JQH51_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY6074648.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46942..47319)
                     /locus_tag="JQH51_000151"
     CDS             complement(46942..47319)
                     /locus_tag="JQH51_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY6074649.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="JQH51_000152"
     CDS             complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="JQH51_000152"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY6074650.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48014..50199
                     /gene="fhuE"
                     /locus_tag="JQH51_000153"
                     /pseudo
     CDS             48014..50199
                     /gene="fhuE"
                     /locus_tag="JQH51_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="JQH51_000154"
     CDS             complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="JQH51_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY6074651.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51987..52784)
                     /locus_tag="JQH51_000155"
     CDS             complement(51987..52784)
                     /locus_tag="JQH51_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6074652.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52795..53799)
                     /gene="holB"
                     /locus_tag="JQH51_000156"
     CDS             complement(52795..53799)
                     /gene="holB"
                     /locus_tag="JQH51_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY6074653.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53796..54437)
                     /locus_tag="JQH51_000157"
     CDS             complement(53796..54437)
                     /locus_tag="JQH51_000157"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY6074654.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="JQH51_000158"
     CDS             complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="JQH51_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY6074655.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="JQH51_000159"
     CDS             complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="JQH51_000159"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY6074656.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="JQH51_000160"
     CDS             complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="JQH51_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY6074657.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="JQH51_000161"
     CDS             complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="JQH51_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY6074658.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="JQH51_000162"
     CDS             complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="JQH51_000162"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY6074659.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="JQH51_000163"
     CDS             complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="JQH51_000163"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY6074660.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59848..60801)
                     /locus_tag="JQH51_000164"
     CDS             complement(59848..60801)
                     /locus_tag="JQH51_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY6074661.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="JQH51_000165"
     CDS             complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="JQH51_000165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY6074662.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="JQH51_000166"
     CDS             complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="JQH51_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY6074663.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="JQH51_000167"
     CDS             complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="JQH51_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY6074664.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62965..63549
                     /gene="yceF"
                     /locus_tag="JQH51_000168"
     CDS             62965..63549
                     /gene="yceF"
                     /locus_tag="JQH51_000168"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY6074665.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="JQH51_000169"
     CDS             complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="JQH51_000169"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY6074666.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65193..68384
                     /gene="rne"
                     /locus_tag="JQH51_000170"
     CDS             65193..68384
                     /gene="rne"
                     /locus_tag="JQH51_000170"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY6074667.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="JQH51_000171"
                     /pseudo
     CDS             complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="JQH51_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="JQH51_000172"
                     /pseudo
     CDS             complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="JQH51_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="JQH51_000173"
     CDS             complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="JQH51_000173"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY6074668.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72184..73281)
                     /locus_tag="JQH51_000174"
     CDS             complement(72184..73281)
                     /locus_tag="JQH51_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY6074669.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="JQH51_000175"
     CDS             complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="JQH51_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY6074670.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="JQH51_000176"
     CDS             complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="JQH51_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY6074671.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74964..75715)
                     /locus_tag="JQH51_000177"
                     /pseudo
     CDS             complement(74964..75715)
                     /locus_tag="JQH51_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(75735..77594)
                     /gene="flgD"
                     /locus_tag="JQH51_000178"
                     /pseudo
     CDS             complement(75735..77594)
                     /gene="flgD"
                     /locus_tag="JQH51_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="JQH51_000179"
                     /pseudo
     CDS             complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="JQH51_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="JQH51_000180"
     CDS             complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="JQH51_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY6074672.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78584..79243
                     /gene="flgA"
                     /locus_tag="JQH51_000181"
     CDS             78584..79243
                     /gene="flgA"
                     /locus_tag="JQH51_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY6074673.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79319..79612
                     /gene="flgM"
                     /locus_tag="JQH51_000182"
     CDS             79319..79612
                     /gene="flgM"
                     /locus_tag="JQH51_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY6074674.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79617..80033
                     /gene="flgN"
                     /locus_tag="JQH51_000183"
     CDS             79617..80033
                     /gene="flgN"
                     /locus_tag="JQH51_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY6074675.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="JQH51_000184"
     CDS             complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="JQH51_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY6074676.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81808..82641)
                     /locus_tag="JQH51_000185"
     CDS             complement(81808..82641)
                     /locus_tag="JQH51_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY6074677.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLCD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82643..83290)
                     /locus_tag="JQH51_000186"
     CDS             complement(82643..83290)
                     /locus_tag="JQH51_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY6074678.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="JQH51_000187"
     CDS             complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="JQH51_000187"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY6074679.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84121..85329
                     /gene="mdtH"
                     /locus_tag="JQH51_000188"
     CDS             84121..85329
                     /gene="mdtH"
                     /locus_tag="JQH51_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY6074680.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85393..86040
                     /gene="grxB"
                     /locus_tag="JQH51_000189"
     CDS             85393..86040
                     /gene="grxB"
                     /locus_tag="JQH51_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY6074681.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86174..86734
                     /locus_tag="JQH51_000190"
     CDS             86174..86734
                     /locus_tag="JQH51_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6074682.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86840..87886
                     /gene="pyrC"
                     /locus_tag="JQH51_000191"
     CDS             86840..87886
                     /gene="pyrC"
                     /locus_tag="JQH51_000191"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY6074683.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87960..88205
                     /gene="dinI"
                     /locus_tag="JQH51_000192"
     CDS             87960..88205
                     /gene="dinI"
                     /locus_tag="JQH51_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY6074684.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88495..88749
                     /gene="bssS"
                     /locus_tag="JQH51_000193"
     CDS             88495..88749
                     /gene="bssS"
                     /locus_tag="JQH51_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY6074685.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88864..89982
                     /gene="solA"
                     /locus_tag="JQH51_000194"
     CDS             88864..89982
                     /gene="solA"
                     /locus_tag="JQH51_000194"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY6074686.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90030..90143
                     /locus_tag="JQH51_000195"
     CDS             90030..90143
                     /locus_tag="JQH51_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY6074687.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90404..90970
                     /locus_tag="JQH51_000196"
     CDS             90404..90970
                     /locus_tag="JQH51_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY6074688.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90974..91549
                     /locus_tag="JQH51_000197"
     CDS             90974..91549
                     /locus_tag="JQH51_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY6074689.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91591..92643)
                     /locus_tag="JQH51_000198"
     CDS             complement(91591..92643)
                     /locus_tag="JQH51_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY6074690.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92868..93788
                     /locus_tag="JQH51_000199"
     CDS             92868..93788
                     /locus_tag="JQH51_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY6074691.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93960..94157
                     /locus_tag="JQH51_000200"
     CDS             93960..94157
                     /locus_tag="JQH51_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY6074692.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94181..>95092
                     /locus_tag="JQH51_000201"
     CDS             94181..>95092
                     /locus_tag="JQH51_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6074693.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT        22884 a        25208 c        23473 g        23527 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agtagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgcgtggtgt ggttctgata
    15781 ttcctactct gctaatttgc tcagccccat gtctgcgtaa gtgcgattta ctgcatttct
    15841 caggtctgcg tagttctccg ttggcggctc cggtgtgccc ttgctgctct gttttaccag
    15901 cgggtcgccc accagctgcc cgtaagctgc aatttgtgct gtcatattgc cacctcagtg
    15961 aagcgatccg gcattgttct ttttcatctc ttccatcatt ttttcagtga gcattacgca
    16021 ctgaaacccc atgtttttga tgtctacggg tttcatgcct ttgtcagtgg atatcacagg
    16081 cagaatatcc tgatacacat cgcggtcagg gtaactaagc acacaaagct cccatgatgc
    16141 cacgggtgag gatccttcgt ctgtttcaac ccaccagcct ggtgtagctg cgcttatttg
    16201 cagaatcgtg atccgtccgt tgtgcttgta aaatttttca ccaaattttg gttcgctcat
    16261 gattccacct ctccggtttt aacgttgatt gttgttacct gttccgcttc ggcaatctcc
    16321 cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca tgaatgcgct tatcagttcg
    16381 ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta tcgttctgat tgccaccttt
    16441 aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt ctgttctgtc tctgcgtttt
    16501 ttgaatgatt tattaaattt ccctgaagtg tgcatattta tttttacccc ctcgtttaaa
    16561 aagttttgag ttgtgcctcc ccttgtctac cttatctacc ttagtggccc tcatgccagt
    16621 aatggcgctg ctttcagcgg ggtagagtgc ttttatccac tatctacccc gtgtctacct
    16681 ccctgtctga ttcaggtaaa atcaggtaga gagggtagat agtgggtaga cagtaaaaaa
    16741 aggctatcta cctaacttaa tgcactgaat taaatgtatt tttctttact caggtagaca
    16801 gggtagacag caattacaaa aaattataaa aacgcgtcgc actcgtctgt tgttattgcg
    16861 ttagtctgcg ttactccctt aactttccgc gtaatatatt catgtccgta aactttcgcg
    16921 gctggcttca tagccttgcc aaagtcattt acgtttagcg gtttgctcct gcctgcgtac
    16981 gccataaacg ccagatagac gcggtaaagg ctgtttctgg tcgtgtactt cactgaatcg
    17041 ccaccgccgc ccatcatcag gccgcgtgct tcctccagaa aattcaggaa ctggcaaaac
    17101 tcaataaccg gatccgtctg ttgctttatt gccagagctt catcaccgtc acgctgttcc
    17161 agtagtaaag cccgtgcttt ctcagagtcg gtaaagttcg ccagcaatcg gcggataatg
    17221 acagggattt cagccgcaat cttttccggt agctccctgt ctttttcggc ctcactaacg
    17281 atattgtcga aacggaaaat cacgcgacga cgtgccacac ctccggcccg ttcggtgaat
    17341 atcatcgggt tattgttggt cgccagcacc accgccctga ttacagccgt gaaacgcttt
    17401 tcgtatttcg ggttaatttc cacggggtca ccgcccgtga ttttcttgat gcccgttcct
    17461 tcgcctgtat atttcggctg gtcagccagg acgataagac gactcccgac aacctgcgca
    17521 cgtccaccag catcatcaag cgatgtcatt tcagcgctta ccgtgttctg tttccctgcc
    17581 agaaggctgg ctatgtgtgt gaatgtactt ttaccgctcc cgccgtctcc ggtggcctca
    17641 ataaacatct gccagtcgta ccggttcgcc ataatcatgt acagcgcggc acatatacgc
    17701 atcatcttgc gctggtcttt tccggctgcg tgctcaagcc atttatgaaa gtttagcgcg
    17761 ttatcgcgga tgttctcccc tggtgctggt ggcgtgtact caatgccgtt gtgcgtggtg
    17821 atccagttct ccggcgtgtg cggggaaaat tcccccgttt tcaggtcaag cgcaccattg
    17881 gcgaacggca gcaaatcgcc ggacggctcg cccattggtt cggcaataac ttttaacgct
    17941 tccacggcgt tattgattac gcgcttgctg aaagtggccc tgtgctctga atagatcacc
    18001 accatttcgc ggctaagttc cattgtgctg accggacacc ataccccgcc gcgccatacg
    18061 tgaacgattt cactttcctg atgcacacaa acgccatcaa agcgctcggc aagcagctgc
    18121 gcgcgctcac tgtctgccat ttgtgccagt tgcgtatttt tctgctccgg catggtaagc
    18181 cctgcggcaa tattttcacg ctcagcattc agatagcgcc gccagttttc acacttctgg
    18241 acgtgcattc cttcggggta aaaatttgcg tcctgaatat ctgccgccgc cagcttctga
    18301 ccaatctttt tggtctccac caaatccagt tctccggcct ggtacagcct tacgcgtttt
    18361 tttccatccg gaacaatttt cagtgcatca agttcggcaa gctgatttgg cccaagccac
    18421 acaggcggca cattatcgcc ggatgcgggg ccatcctgct cctgccattg tttcgcgtgc
    18481 gaccaggcat cactacccgc aaagataatt acctctgtgt ctttgtgttt tattccgcgt
    18541 ggctgttttt ttacgttcgg tgccagtttc attttttacc cctgaatacg ttaagcatct
    18601 tttttatttc ctgaatattg gcgcgtgctt tctccctgct ggttggttta ctgcggggcg
    18661 ctgcctgtac cagcgtgaaa tcacgccgga attgataaac aggcatcacg caatcatatt
    18721 cgtaaccttc acggcggtag gtgatgcgcc gttctgccac gcctttaatc gttaccatgc
    18781 cgccgtactg gtcgcggtaa atatcaccgc gcgtaaattt agggtgagtg ttgccactgg
    18841 cagttaagcc agaatattta agtttcatta tttttattct ccggtgtgtt gttctttata
    18901 tctgtcgtgc aatagatcta tttcttgcag ttccattatt acaggctcaa gaagcgttat
    18961 taatgccgtg gcaattcttg atttttgttt gtcgcgttca ttgtcgccaa gtgtttcaag
    19021 ccatatgcgc aatatttcca gcatgttttc actgtgagaa agtgcaagaa atgcgcggtc
    19081 tattgtttcg tggtaaatat cacgcatatt aatccccgtc cgtcgttttt cttaaaataa
    19141 cctctgtcac gaaatcagca taatcggcgg cgatattcag tacatcaagc ccgttgattt
    19201 atattctctc gtggaaagta agaaaaaagc cgctctaata agttctggca ttgacgaaag
    19261 cgcatcagcc gcatcatcag gaacgccgga aaattcctgt ttcagggaat taaaacgatc
    19321 atcacgcata acccccccca ttttcacaat cggcaacaag aatattttta gcgtcattca
    19381 gtgaccgcgt tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg tctttctgtt
    19441 cttctccctg cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc atcagcgtag
    19501 tgagtgcggc ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc cccctgacga
    19561 atacgggcgg cgaatacagc aacacaaccg gacgggcaac ggctacgcgc ttcgcgttcc
    19621 gtccaagcgg ttacgtggat gatttgggat tctccggcac tcagtgccag aaaacgccac
    19681 acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct
    19741 cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg ggctttgctt
    19801 tttgtgttga ttgtgtgtaa tgtgtcacta cacataaata cattacctga ggtgtaatta
    19861 acgtgtcaac acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc cgtttagatc
    19921 ctgacttacg ggaaaagatg gaaaaggcac aacaacagga cggcgatgat tctcttgctg
    19981 cctggataaa gcgaataatc cgcaaagaat tacagcaacg cggcatagaa cccaaaggat
    20041 aaaaactatc agcgccgtgg tgtgaggtac tacggcgcat tgctttacag gcgcacacaa
    20101 tgaccaacga agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga
    20161 aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag
    20221 aatactcatc aaaggagaga acttcgcagt tatggcaacg cactgaacca gactgattgc
    20281 attgcccacc agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac
    20341 aaacggcgca gcgggttatc tgttcagaaa ggcggctcca tttcggggcc gcttttttta
    20401 tgcctgaaaa accccaattt tgtggttttc cagtttcacc agggcgaacg aatccccgcc
    20461 cacgttctgg cgtatattca atcttcatgg ttatagctct gtgttcagat gattgatgtg
    20521 tggcggctgc gtgccgccag cgtgattaat gaactgcctt gcagctatcc ttccaggcca
    20581 gaacctcgga taaagaccag ccaacggaac gaccgccaag tttacgacgt gatgggaatt
    20641 gtccggcctt ttccaggcgg tagcggcatg agcggctaag gcctgttagc ttttcgcatt
    20701 ctttttcacg tataaaccga tcagtgctta acactattgc cccctttcgt ttcttaaaga
    20761 gttatttcgt gttctattgc gttgggatgt gtctgattgt gtcaggatga ttcagagttg
    20821 gcaaatgttg aggccgtatg gtttacagaa agaggaataa tcaggataaa atcctttaaa
    20881 ttcatgttaa tacaaaggca taaaatattg tttcatgcct tttttctcgc tatttaaaga
    20941 gtgattcgct agtgtataaa aaatcagtaa cacattaaaa atcagctact tataaatctg
    21001 tacacttttt cacctcttgt tcgtagttgt tccgcattgt tgctcattgt tgcacgttgt
    21061 atctgttcgc atatccagta tgcgcatact gaaaaaacac gaaaaaaatt attttcttct
    21121 ggctactggt agcgtggtta cgttttcatg tgttcccgcc agtatcccta accgctccgt
    21181 ccacatatcc agcgcattgc gtttagcatc cagataacgg gaatgattat aaactcgctg
    21241 cattcctggc atctggtggc ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc
    21301 gttcagcatc gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag ggtgatttag
    21361 cctcctgtgc gctaatctgc cgtactgcga cacgcttgtt tcctgtttca cttcccccag
    21421 caataagccc gtgtgcctgt tctgctccac cagctgcgtg acgaacggca gtattgcttc
    21481 cggtatgggc cggaatattg ccaccttcgt tttgctgtgt tctttcggca cggtccagag
    21541 catttcggta aaatcccact cgctgatctc cgataacctc agttctaccg tccggcatcc
    21601 gaacacaatc aggaggcgga ttaacgcgat gtagtagggg gagaatattt ttttgtccag
    21661 tgcctgcaat aattcgccca gttctttggt gcttaagaca cgctcgctta tatccggttt
    21721 tttcccaacg tccgccacac tcatatcatc aagaacgttg ctgattgcat agcgccgcct
    21781 ccggcagaac ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag taacaggcgt
    21841 tcgctttgcc acctggtcaa aacaggccag ccagtgccgt agctcgcatt tatccagcgg
    21901 catagcacca atgtgctgta ttacgtgatt attaaggcgc tttttcaggg cggcataatc
    21961 cacgcggttt tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc caaccgttac
    22021 cggctttaac gcttcctgta cggtgtaatt aagctcatga cgtggatttt tcccctctgc
    22081 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggctca gatcaggata
    22141 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
    22201 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
    22261 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
    22321 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
    22381 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
    22441 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
    22501 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
    22561 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
    22621 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
    22681 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
    22741 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
    22801 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
    22861 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
    22921 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
    22981 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
    23041 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
    23101 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
    23161 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
    23221 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
    23281 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
    23341 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
    23401 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
    23461 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
    23521 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
    23581 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
    23641 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
    23701 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
    23761 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
    23821 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
    23881 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
    23941 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
    24001 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
    24061 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
    24121 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
    24181 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
    24241 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
    24301 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
    24361 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
    24421 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
    24481 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
    24541 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
    24601 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
    24661 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
    24721 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
    24781 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
    24841 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
    24901 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
    24961 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
    25021 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
    25081 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
    25141 aagcggtttg ccgtagaatt accgccctca ttaggagtca ccaaggtggt taaccacaaa
    25201 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
    25261 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
    25321 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
    25381 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
    25441 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
    25501 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
    25561 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
    25621 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
    25681 gtggtcaaca gcaacgcgtc gccattgctc gagcggtggt taacaagcct cgtctgttgt
    25741 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
    25801 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
    25861 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
    25921 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
    25981 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
    26041 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
    26101 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
    26161 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
    26221 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
    26281 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
    26341 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
    26401 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
    26461 acccgcgacg acgccagttt cgtcaaaatg gtctttacac tggataacta cacgcgtctg
    26521 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
    26581 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
    26641 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
    26701 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
    26761 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
    26821 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
    26881 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
    26941 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
    27001 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
    27061 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
    27121 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
    27181 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
    27241 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
    27301 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
    27361 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
    27421 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
    27481 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
    27541 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
    27601 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
    27661 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
    27721 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
    27781 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
    27841 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
    27901 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
    27961 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
    28021 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
    28081 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
    28141 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
    28201 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
    28261 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
    28321 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
    28381 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
    28441 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
    28501 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
    28561 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
    28621 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
    28681 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
    28741 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
    28801 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
    28861 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    28921 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    28981 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    29041 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    29101 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    29161 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    29221 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    29281 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    29341 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    29401 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    29461 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    29521 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    29581 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    29641 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    29701 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    29761 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    29821 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    29881 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    29941 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    30001 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    30061 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgtg gaacacgagc
    30121 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    30181 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    30241 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    30301 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    30361 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    30421 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    30481 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    30541 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    30601 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    30661 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    30721 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    30781 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    30841 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    30901 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    30961 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    31021 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    31081 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    31141 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    31201 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    31261 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    31321 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    31381 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    31441 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    31501 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    31561 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    31621 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    31681 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    31741 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    31801 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    31861 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    31921 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    31981 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    32041 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    32101 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    32161 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    32221 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    32281 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    32341 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    32401 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    32461 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    32521 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    32581 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    32641 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    32701 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    32761 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    32821 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    32881 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    32941 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    33001 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    33061 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    33121 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    33181 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    33241 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    33301 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    33361 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    33421 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    33481 tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    33541 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    33601 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    33661 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    33721 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    33781 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    33841 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    33901 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    33961 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    34021 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    34081 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    34141 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    34201 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatatttt atcatcaatc
    34261 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    34321 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    34381 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    34441 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    34501 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    34561 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    34621 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    34681 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    34741 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    34801 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    34861 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    34921 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    34981 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    35041 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    35101 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    35161 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    35221 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    35281 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    35341 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    35401 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    35461 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    35521 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    35581 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    35641 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    35701 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    35761 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    35821 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    35881 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    35941 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    36001 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    36061 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    36121 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    36181 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    36241 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    36301 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    36361 cattgttctt cagcgaacca ctgccaccgc tgttcagtta tttccctgcc aataccttgc
    36421 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc
    36481 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    36541 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    36601 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    36661 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    36721 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    36781 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    36841 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    36901 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    36961 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    37021 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    37081 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    37141 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    37201 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    37261 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    37321 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    37381 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    37441 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    37501 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    37561 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    37621 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    37681 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    37741 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    37801 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    37861 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    37921 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    37981 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    38041 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    38101 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    38161 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    38221 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    38281 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    38341 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    38401 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    38461 aacaaagcgg ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    38521 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    38581 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    38641 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    38701 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    38761 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    38821 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    38881 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    38941 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    39001 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    39061 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    39121 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    39181 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    39241 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    39301 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    39361 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    39421 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    39481 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    39541 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    39601 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    39661 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    39721 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    39781 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    39841 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    39901 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    39961 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    40021 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    40081 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    40141 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    40201 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    40261 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    40321 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    40381 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    40441 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    40501 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    40561 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    40621 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    40681 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    40741 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    40801 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    40861 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    40921 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    40981 agcgtggagt ctgcgctggc tgactatttt gctgccatcg ccaactgctt taccagcaaa
    41041 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    41101 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    41161 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    41221 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    41281 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    41341 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    41401 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    41461 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    41521 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    41581 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    41641 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    41701 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    41761 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    41821 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    41881 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    41941 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    42001 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    42061 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    42121 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    42181 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    42241 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    42301 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    42361 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    42421 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    42481 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    42541 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    42601 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    42661 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    42721 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    42781 gcagcagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    42841 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    42901 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    42961 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    43021 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    43081 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    43141 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    43201 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    43261 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    43321 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    43381 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    43441 acaatcacaa tctttttcaa tggcgtagtc aacgtgcccc ccttaaattt attaaccaat
    43501 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    43561 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    43621 aagttttgtt gcttaaaaga taggcaactt taccggggga gatcaaaaaa tagagtttat
    43681 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    43741 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    43801 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    43861 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    43921 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    43981 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    44041 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    44101 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    44161 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    44221 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    44281 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    44341 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    44401 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctactttc
    44461 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    44521 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    44581 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    44641 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    44701 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa ccccagttcc
    44761 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    44821 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    44881 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    44941 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    45001 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    45061 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    45121 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    45181 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    45241 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    45301 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    45361 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    45421 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    45481 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    45541 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    45601 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    45661 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    45721 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    45781 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    45841 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    45901 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    45961 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    46021 cgcaccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    46081 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    46141 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    46201 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    46261 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    46321 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    46381 cagaggcgca ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    46441 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    46501 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    46561 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    46621 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    46681 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    46741 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    46801 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    46861 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    46921 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    46981 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    47041 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    47101 ggtaacgggt tcttgccttt cgttataaat cgttgaggat gctgaaggtt gaatatcaga
    47161 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    47221 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    47281 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    47341 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    47401 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    47461 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    47521 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    47581 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    47641 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    47701 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    47761 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    47821 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    47881 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcagc aaaattattc
    47941 gcacactaaa tataaccgct catcaccctc ctccggcatg agcctgacga caacataaac
    48001 caagagattt caaatgcttt caacacaatt taacagggat aatcaatatc aagtcatcac
    48061 caaaccgtca ctacttgccg gttgcatagc actggcacta ttaccttctg ccgcttttgc
    48121 tgcaccagcc actgaagaaa cggtgattgt tgagggttca gccacagctc cagatgatgg
    48181 cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga tgactcaaca
    48241 tgatattcct cagtcggtca ctattgttag ccagcagcgg atggaagatc agcagttaca
    48301 aacgctgggc gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg cggattccga
    48361 tcgtgctctt tattattccc gcggattcca gatcgataac tatatggttg atggtatccc
    48421 cacctatttt gaatcgcgct ggaatctggg cgacgcactt tctgatatgg cactgtttga
    48481 acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc
    48541 ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa tttaaaggcg atgtctcggc
    48601 ggaatacggt agctggaaca aagaacggta tgtggcggat ttacaaagcc cactcaccga
    48661 agacggtaaa atccgcgcgc gaattgtcgg cggctaccag aataacgact catggctgga
    48721 ccgctacaat agtgaaaaga ccttcttctc gggcattgtc gatgctgatt taggcgatct
    48781 tacgacgctt tcagccggtt acgaatatca gcgcattgat gttaatagcc caacctgggg
    48841 cggtttaccg cgctggaata ctgatggcag cagcaacagt tacgatcgcg cacgcagtac
    48901 cgcacctgac tgggcgtaca acgataaaga gatcaacaag gtctttatga ccctgaagca
    48961 gcggtttgct gatacctggc aagcgacact gaatgccacc cactctgaag tcgaatttga
    49021 cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc
    49081 atacagtaat tatggacctg gctttgatca tgtcggcggc accggttgga acagtggcaa
    49141 acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt tatgaattgt ttggtcgtca
    49201 gcacaatcta atgtttggtg gcagttacag caaacaaaac aatcgttcag ttcatgggcc
    49261 aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaagcc
    49321 gactggtcat cacagagcct ggcgcaggac gataccacac atatgaaatc gttatatgct
    49381 gccactcgtg tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac
    49441 tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt
    49501 ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc tattttccag
    49561 ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac
    49621 gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc
    49681 cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc
    49741 gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac
    49801 ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatct tgcagaggat
    49861 aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc
    49921 tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc
    49981 gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg
    50041 ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc
    50101 aataacctgt tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca
    50161 ccgcgtaatt tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca
    50221 gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca
    50281 tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tagcctgaac accagaacca
    50341 gcaaccacta cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca
    50401 tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta
    50461 ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct
    50521 tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag
    50581 atggtgtagt aaacaatcgc ataaccgata ccgacgatcg gtaacagcca cagtttgctg
    50641 ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt accgtcacgc
    50701 atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac
    50761 aggatcggcg caacgaacat gaagaagaac tcgatcggct cggtgatacc ggtcaggaac
    50821 gaggtcagcg ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca
    50881 gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaagccacca
    50941 gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc
    51001 tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg
    51061 tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc
    51121 gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa
    51181 atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag
    51241 cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac
    51301 atgtacgctg caatcgcacc ggagataatc cctccgagta cgccagtatc cgccaagtgt
    51361 ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt
    51421 ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta
    51481 aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct
    51541 gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg
    51601 cccagcagaa tacctgcgat aggtagtacg gataccggca gcatcagcga tttaccgacc
    51661 ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga gtatgggtgc
    51721 tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa
    51781 gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt gacggctatc
    51841 acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata
    51901 tttcaaacgc ggttattcac cgcccaattt tcggcggtga actttactcg ttttagccat
    51961 taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg
    52021 tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct
    52081 ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct
    52141 cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg atccaggggg
    52201 acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat gccggaaaag
    52261 gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag
    52321 tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggca
    52381 cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga
    52441 tggatgaagg actcttgctg acgtacttta gtttccggtg tgtaataata atccagcccg
    52501 gtttcaccca gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg
    52561 tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg
    52621 cctaccagat cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat
    52681 ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca
    52741 taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag
    52801 atgaggaacc ggtaacacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc
    52861 ggtgatgaga agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca
    52921 aacatccccc agtatagcct gcaggcacga gggagaaaga tggtttgcca gttcggcgac
    52981 caggcccggc acatcaacat tggtcacctg cgcagcgcca tgatggcgtt ttagcgcatc
    53041 catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat taagggccgc
    53101 cagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac acaatgtttc
    53161 acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct
    53221 taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaagt
    53281 cacggcgtac tgttctggag gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt
    53341 tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caggtttctg ctggtggctc
    53401 ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac
    53461 ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg
    53521 taccgcatca atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg
    53581 atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg
    53641 cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc
    53701 cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag
    53761 tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag
    53821 tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg
    53881 gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag
    53941 aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag
    54001 ccaacttccg gggtaacatc gagatagagc gttaagtctg ggcgaaaatc cccgagaaca
    54061 gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc
    54121 tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc cgttagccag cgcaggtttg
    54181 atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg
    54241 gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca
    54301 agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc
    54361 gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg
    54421 atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt
    54481 atgactggca agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag
    54541 atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat
    54601 cgcacccggc ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc
    54661 tgcacgagaa agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt
    54721 ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc
    54781 actggcaacg gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc
    54841 tttataaggc agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt
    54901 cttgtgaact cgcttgagta acgcgacatc ggtggtattg gcggtataca tcaaggtgtc
    54961 tggccagaac caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt
    55021 ggcgtactta tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag
    55081 gtagtcgctc agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct
    55141 ttccaacaat ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc
    55201 tttaaagtga gaaagatccg gttcgatacg cagcagccat tgaaaaaccc gtgggcgatt
    55261 gatgatctta tcagcataaa gctgttcacc gagcgccaga cgtccggtcc ctggcttcag
    55321 ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca
    55381 gacgcccaca ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac
    55441 ttttttcatg actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct
    55501 gacgaaaagg tgacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa
    55561 ataaccatct catctgcctg caacgcctcc tccagcgagg cttgcacttc gacaagctga
    55621 taagaggatt gtgccagtaa acggatacag aattgtcgca taatgccgtt aacacctgcc
    55681 tgatccagtc gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag
    55741 cattccgtaa cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca
    55801 agatgagagc gaatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga
    55861 ggattgcgcc ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg
    55921 tcgtaatgtg caggataagc cgtaacggag agaatccgcg ttgctggtcc gctgttcaat
    55981 gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc
    56041 tgttctgctg ccagcgtttt catctcttgt tcaagctgag gccagaagtc acaggaaatc
    56101 atcaaccgct gacaagcatc ctgtagtcgc aggatatgcg ccgataacaa actgacttta
    56161 ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt
    56221 actgccagcg attcctgctt ataaccgtta attaagaaca taccggctcc ttatcagaga
    56281 attgttagtg tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag
    56341 gcccgcaagc ggacctttta tatgggtgga aaatgacaac ttagatcttt ttaaagatca
    56401 aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga
    56461 cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt
    56521 tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg
    56581 cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc
    56641 ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg
    56701 tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca
    56761 gagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat
    56821 aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt
    56881 tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat
    56941 cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg
    57001 cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg
    57061 caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca
    57121 cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag
    57181 tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac
    57241 gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc
    57301 cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat
    57361 cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct
    57421 gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg
    57481 ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca
    57541 gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca
    57601 caactacacg acgcttagac acgtttgtcc tccaaggagg gaaaaatgat tctagtggga
    57661 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
    57721 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
    57781 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
    57841 cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
    57901 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
    57961 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
    58021 aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg
    58081 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
    58141 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
    58201 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
    58261 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
    58321 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
    58381 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
    58441 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    58501 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    58561 gttagtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    58621 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    58681 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttggc
    58741 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    58801 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    58861 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    58921 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    58981 caggccagta agcaccttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    59041 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    59101 acggatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa cagaaactgt
    59161 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    59221 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgccagcagc
    59281 tttacaggca gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc
    59341 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    59401 cgcaataggc gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttccggtac
    59461 ggcttcttgc atgaacttac cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    59521 cacgccagcg caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    59581 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    59641 tggctgggtt tgccaggttt tattcagttc ttcagctgac ccctgctggg tcagcgccca
    59701 caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    59761 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    59821 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcagagc cccaagtgaa
    59881 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    59941 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt
    60001 caccacgaca ttgtccatcg acataccgag ttttttcgcc gttgcactga taatacgcag
    60061 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    60121 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    60181 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    60241 ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg attcccggct cttcagaggc
    60301 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    60361 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    60421 tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa cctgcacagg ctgctgcaac
    60481 gtcaaatgcc gggcaacctt taatgtccaa catgctttga atctgacaag ctgcgctcgg
    60541 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    60601 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    60661 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    60721 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    60781 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    60841 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    60901 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    60961 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    61021 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    61081 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    61141 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    61201 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    61261 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    61321 ggattgtttt aagcaccgct gaggcatccc gaatactgtc aagacccttt acttcttctt
    61381 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    61441 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    61501 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    61561 gctcaatccc ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat
    61621 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    61681 ttgaactccc acggctggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    61741 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    61801 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    61861 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    61921 ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    61981 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    62041 gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt
    62101 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    62161 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    62221 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    62281 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    62341 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    62401 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    62461 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    62521 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    62581 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    62641 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    62701 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    62761 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    62821 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    62881 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    62941 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    63001 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc tgaggtcgac
    63061 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    63121 tcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    63181 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    63241 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    63301 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctaagcgag
    63361 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    63421 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgatcc taacacgctg
    63481 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    63541 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    63601 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    63661 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    63721 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    63781 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    63841 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    63901 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    63961 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    64021 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg
    64081 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    64141 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    64201 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    64261 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    64321 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    64381 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    64441 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcacctt tacgcaaaat
    64501 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg
    64561 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    64621 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttattttt
    64681 agttttgcac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    64741 ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct
    64801 ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg tccatccttg ttaaaacaag
    64861 aaattttacg gaataaccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac
    64921 gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa
    64981 tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga
    65041 aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac
    65101 gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta
    65161 gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc
    65221 agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctggatatcg
    65281 aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg
    65341 aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac
    65401 taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca
    65461 ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg
    65521 gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga
    65581 tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg
    65641 aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca
    65701 ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac
    65761 actgggaagc catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg attcatcagg
    65821 agagcaacgt aatcgttcgc gccttccgcg attatttacg tcaggatatc ggcgaaatcc
    65881 ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc
    65941 cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc
    66001 agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca tccggtggtt
    66061 ccattgttat cgacagcacc gaagcgttaa cagccatcga catcaactcc gcacgcgcga
    66121 cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc gccgatgaga
    66181 ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca
    66241 tgacgccagt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg
    66301 accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgcc
    66361 agcgcctgag cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta
    66421 ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg atcgaagaag
    66481 aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtgcca atcgcttctt
    66541 atctgctgaa tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag gacggtgttc
    66601 gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc
    66661 gcaaagggga agaaacgccg accttaagct acatgctgcc gaagctgcat gaagaagcga
    66721 tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg
    66781 caacctttgc catgccggat gtaccgccag cgccaacccc agctgaacct gccgcgactg
    66841 tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc agctcctgca caacctgggc
    66901 tgttaagccg cttcttcggc gcactgaaag cgctgttcag cggtggtgaa gaagccaaac
    66961 cgaccgagca accaacaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca
    67021 aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta
    67081 ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaaa
    67141 ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg
    67201 acgagcagca agtgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg
    67261 cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg
    67321 aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc
    67381 gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg
    67441 ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc
    67501 tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg
    67561 acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc
    67621 agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg
    67681 tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac
    67741 gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga
    67801 tggtgactga ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg ccagttgttg
    67861 aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg
    67921 ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta
    67981 ccgagtctga tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg atggttgaac
    68041 cgcaggaaga gacggcagat gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    68101 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    68161 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    68221 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    68281 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    68341 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    68401 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    68461 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    68521 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    68581 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    68641 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    68701 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    68761 ctccagtttg ttcagctgcg tgcctaattc cgcgcgaacg gtaaggacat tattcagcga
    68821 gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt tatccgcttc
    68881 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    68941 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    69001 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    69061 ttctgttccg ccaacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    69121 tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct
    69181 gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga
    69241 caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca ctctcttcca
    69301 gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt tgcgcctgtg
    69361 cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg ttaacgactc
    69421 gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa ttggtgatgc
    69481 cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg
    69541 aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag
    69601 gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat
    69661 cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt
    69721 caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga
    69781 tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg
    69841 gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt cgtcggtgat
    69901 taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac
    69961 ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc
    70021 tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc
    70081 gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg tggcaccgat
    70141 cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagtaaagaa
    70201 atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt tgaaagcctc
    70261 ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat cctgagaacg
    70321 gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt
    70381 gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac
    70441 tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg catcgtgatg
    70501 ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt taattcgctc
    70561 accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc
    70621 aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca
    70681 acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg
    70741 gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgccgccggg
    70801 tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc
    70861 agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg
    70921 gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgtatcatac
    70981 tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg
    71041 ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag
    71101 atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta
    71161 ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag
    71221 cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc
    71281 tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt
    71341 gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc gcactcgcgg
    71401 cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg
    71461 cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct tcgccgtttt
    71521 catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag gctttgacac
    71581 caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag
    71641 attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca
    71701 gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc agcgaatcat
    71761 cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt tgataacgca
    71821 ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt
    71881 gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca
    71941 tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc tcgctgctga
    72001 acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc
    72061 cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc gctttcgcct
    72121 ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga
    72181 tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcaaaat
    72241 agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca cgttattgag
    72301 gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt
    72361 ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc
    72421 ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac
    72481 ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg
    72541 ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg aactgttgcc
    72601 acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg
    72661 cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg
    72721 caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc
    72781 attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc ctgcggaggc
    72841 tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc
    72901 cttaagcgga gtcatcaaca acgtacctcc acgcaagctt ttggcattac ccatggaaga
    72961 aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg ctgtcaccat
    73021 taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg
    73081 tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccagt
    73141 gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta ccccctgaac
    73201 actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa
    73261 tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa
    73321 cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaata
    73381 cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta
    73441 accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc
    73501 accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta
    73561 aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg
    73621 acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca
    73681 aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc
    73741 tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc
    73801 ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc
    73861 gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc
    73921 caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg
    73981 tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttat
    74041 gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt
    74101 gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt
    74161 agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc
    74221 gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagtcc
    74281 ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc
    74341 ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg
    74401 aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc cgttctgatc
    74461 cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca ttacctggaa
    74521 aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca gattgccctg
    74581 gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta atccggaggg
    74641 taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat aaagcagatc
    74701 ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca ggttgttggc
    74761 aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact
    74821 gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg cgacagtgtg
    74881 aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg cgcgctttta
    74941 ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc acggcctgcg
    75001 ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc gctggcaatc
    75061 atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc cgacatcaca
    75121 cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc tgcgcttaaa
    75181 cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac cagtttcaga
    75241 cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga gattgtgcca
    75301 tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc ctcgcctatc
    75361 accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct gccattacgc
    75421 gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc aacgcaacgt
    75481 ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg
    75541 ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt
    75601 ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct
    75661 gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga tccattgagc
    75721 tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc ctgggttttg
    75781 atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac cagttcttta
    75841 ctgagatcga cgttggacgc ttccagcgca ccgttggtca gagtgccaaa gtttcccgtc
    75901 ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct
    75961 tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg
    76021 gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg ataactcacc
    76081 agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc gccggttgcg
    76141 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc cagtgttgtc
    76201 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    76261 tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    76321 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    76381 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    76441 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    76501 ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa caggttacgg
    76561 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    76621 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    76681 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    76741 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    76801 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    76861 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    76921 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggatc
    76981 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    77041 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgaaccg
    77101 tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    77161 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    77221 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtt
    77281 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    77341 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    77401 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    77461 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    77521 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    77581 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    77641 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    77701 taaccatctc cccgacgaca tcaacgttcg gcatttttat gtagcccttt gcatctgcca
    77761 gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa catcggcaac
    77821 ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca cctgttttgc
    77881 ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat tactggccgc
    77941 cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    78001 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    78061 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    78121 atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt attgcagttc
    78181 tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc gttgaggtca tcgtcagtgc
    78241 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    78301 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    78361 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    78421 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    78481 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atggtcattg
    78541 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga caataaaacg
    78601 tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca atctcacatc
    78661 gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc
    78721 tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt cgatgagcaa
    78781 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    78841 tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc ctattgtgcg
    78901 gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg ataccctgcc
    78961 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    79021 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    79081 acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct
    79141 gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcggaac aggtagtcag
    79201 cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta aagattaccc
    79261 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    79321 gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac
    79381 cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca gcaccagtgt
    79441 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    79501 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    79561 aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    79621 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    79681 tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    79741 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcaaga
    79801 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    79861 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    79921 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    79981 cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc tgaatattat
    80041 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    80101 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    80161 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    80221 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    80281 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    80341 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg tttcaacgga
    80401 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    80461 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    80521 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    80581 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat gcccaacgca
    80641 accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    80701 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    80761 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    80821 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    80881 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccagca
    80941 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    81001 tacaccagct gcagaatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    81061 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    81121 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    81181 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    81241 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    81301 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    81361 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    81421 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    81481 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    81541 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    81601 aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc aggcgctgaa
    81661 agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt acagggttta
    81721 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctattc
    81781 gccggcggtt tgcggaactg tctgatttta cacgcattca atgaagtgcc gcgcacagcc
    81841 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat tcactacacc
    81901 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
    81961 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag tggtgatctg
    82021 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    82081 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    82141 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc tattgctacg
    82201 atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact caccgtagag
    82261 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    82321 caccagcctt tcagcatcgc acagattttc tgccagcggt ttatcgacac agacatgtac
    82381 ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg agtgcacaaa
    82441 aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    82501 gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta acgtccagtc
    82561 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccactac
    82621 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgtt
    82681 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa tcaccgtcaa
    82741 ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg aaaagatgca
    82801 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    82861 cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    82921 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    82981 tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    83041 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
    83101 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
    83161 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    83221 gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg gcagtaagtt
    83281 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    83341 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    83401 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    83461 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    83521 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    83581 aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    83641 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccctagc
    83701 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    83761 aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg atcatgcacc
    83821 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    83881 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    83941 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    84001 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat actgtccccg aagtgatggg
    84061 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    84121 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    84181 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    84241 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    84301 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    84361 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    84421 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    84481 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
    84541 atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    84601 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg tgcggcgttc
    84661 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    84721 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    84781 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    84841 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    84901 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    84961 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct acaacaactt
    85021 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    85081 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    85141 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    85201 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    85261 ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc
    85321 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    85381 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    85441 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    85501 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    85561 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    85621 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    85681 gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    85741 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    85801 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    85861 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    85921 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctaat
    85981 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    86041 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    86101 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    86161 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    86221 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    86281 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    86341 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    86401 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    86461 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    86521 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    86581 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    86641 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    86701 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    86761 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
    86821 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    86881 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    86941 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    87001 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    87061 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    87121 taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    87181 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    87241 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    87301 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    87361 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    87421 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    87481 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    87541 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    87601 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    87661 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    87721 attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca cattcatcga
    87781 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    87841 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    87901 tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag ggggctatta
    87961 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    88021 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    88081 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    88141 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    88201 aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat tttacttacg
    88261 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    88321 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    88381 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    88441 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    88501 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    88561 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    88621 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
    88681 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
    88741 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    88801 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    88861 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    88921 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    88981 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    89041 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    89101 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    89161 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    89221 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    89281 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    89341 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    89401 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    89461 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    89521 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    89581 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    89641 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    89701 gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    89761 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    89821 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    89881 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga ccaaaaaagc
    89941 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    90001 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
    90061 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
    90121 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
    90181 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
    90241 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
    90301 attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt cattctccga
    90361 cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac
    90421 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
    90481 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
    90541 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
    90601 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
    90661 tcgtcttgcc gcaaaagctg gacatctcgc attgtgccta ttgctgttcg ccatcggcat
    90721 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
    90781 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
    90841 ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg ccctgaagca
    90901 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
    90961 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
    91021 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
    91081 taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta aagatttcga
    91141 cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    91201 caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca gtgcggattt
    91261 tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    91321 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    91381 ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg caggctttga
    91441 ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag gtccagcttc
    91501 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    91561 tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa tacccagtgt
    91621 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    91681 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    91741 acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc agccatcatt
    91801 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    91861 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    91921 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    91981 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    92041 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    92101 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    92161 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    92221 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    92281 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    92341 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    92401 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    92461 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    92521 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc
    92581 gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt gtaacactgg
    92641 catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat atagcaatta
    92701 tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt atttgttatt
    92761 tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat
    92821 gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg acgaatctac
    92881 ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg ggtattggcg
    92941 tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt tgtggattag
    93001 gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac
    93061 tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat
    93121 ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac cgccgaatcg
    93181 cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg
    93241 gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg cagtttggta
    93301 tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt gactggctac
    93361 aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca
    93421 tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat gattacggcc
    93481 cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc acgaccggaa
    93541 cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc
    93601 cagatggcaa agggtatcaa ttgattatgc tgccaccaga gtgttctccg ccactggatg
    93661 atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc atgatggcac
    93721 cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc gttccttcac
    93781 gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc acagcaaatc
    93841 cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg gcgcataatt
    93901 agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag cggattgcta
    93961 tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc gtcgcctggc
    94021 taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta cccctctacg
    94081 ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt attgtcttca
    94141 gtataatggg ttcctaaaca catcatttta ggtattgaat atgagacggg caatcagcta
    94201 catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac aatctaagct
    94261 ggttactgac tggcttgata agaaccctga gttttacttg gattcatcct tgagctttaa
    94321 agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag gtgacttttt
    94381 aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg agagtttaga
    94441 ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta tcttaagggc
    94501 tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa gtttaaaaga
    94561 cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg aaagtttgag
    94621 aaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct ccgaaggtat
    94681 caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc gaacattcac
    94741 aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat tacagggttt
    94801 aagcttttgg gctatcacca gaactttaaa cgatgaaggt cacttgtcat tgaatcaata
    94861 cacacccaaa caaaaggggt ggtctgatac tgctgtaaag aaactgctca gaaatcgtgc
    94921 tgtgattgga tgtttcacac ctgctggacg tgaagaagtt cagggttact accccgcaat
    94981 aatcagtgag tcactattct atcgagtaca gcaactgaac acagggcaat atggtagggc
    95041 ttctgtatca agtaatccgc tatcagtcaa cttgttcagg ggtatcatca aa
//
LOCUS       DADLEE010000003        75676 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000003 DADLEE010000000
VERSION     DADLEE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75676
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.003"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(435..641)
                     /locus_tag="JQH51_000202"
     CDS             complement(435..641)
                     /locus_tag="JQH51_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY6074694.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JQH51_000203"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JQH51_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY6074695.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JQH51_000204"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JQH51_000204"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY6074696.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JQH51_000205"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JQH51_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JQH51_000206"
     CDS             4795..5367
                     /locus_tag="JQH51_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY6074697.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdbD"
                     /locus_tag="JQH51_000207"
                     /pseudo
     CDS             5360..8040
                     /gene="kdbD"
                     /locus_tag="JQH51_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="JQH51_000208"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="JQH51_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="JQH51_000209"
     CDS             8997..9101
                     /locus_tag="JQH51_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY6074698.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="JQH51_000210"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="JQH51_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="JQH51_000211"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="JQH51_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY6074699.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="JQH51_000212"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="JQH51_000212"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY6074700.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="JQH51_000213"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="JQH51_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY6074701.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="JQH51_000214"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="JQH51_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6074702.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16212..16574
                     /gene="ybfE"
                     /locus_tag="JQH51_000215"
     CDS             16212..16574
                     /gene="ybfE"
                     /locus_tag="JQH51_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY6074703.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="JQH51_000216"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="JQH51_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY6074704.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="JQH51_000217"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="JQH51_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY6074705.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="JQH51_000218"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="JQH51_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY6074706.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="JQH51_000219"
     CDS             complement(18063..18389)
                     /locus_tag="JQH51_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074707.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="JQH51_000220"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="JQH51_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY6074708.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="JQH51_000221"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="JQH51_000221"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY6074709.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="JQH51_000222"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="JQH51_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY6074710.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="JQH51_000223"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="JQH51_000223"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY6074711.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="JQH51_000224"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="JQH51_000224"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY6074712.1"
                     /translation="MYALTLGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="JQH51_000225"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="JQH51_000225"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY6074713.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="JQH51_000226"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="JQH51_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="JQH51_000227"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="JQH51_000227"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY6074714.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="JQH51_000228"
     tRNA            31034..31110
                     /locus_tag="JQH51_000228"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="JQH51_000229"
     tRNA            31119..31203
                     /locus_tag="JQH51_000229"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="JQH51_000230"
     tRNA            31227..31301
                     /locus_tag="JQH51_000230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="JQH51_000231"
     tRNA            31336..31410
                     /locus_tag="JQH51_000231"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="JQH51_000232"
     tRNA            31426..31502
                     /locus_tag="JQH51_000232"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="JQH51_000233"
     tRNA            31551..31625
                     /locus_tag="JQH51_000233"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="JQH51_000234"
     tRNA            31663..31737
                     /locus_tag="JQH51_000234"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="JQH51_000235"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="JQH51_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY6074715.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="JQH51_000236"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="JQH51_000236"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY6074716.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34750..35829
                     /locus_tag="JQH51_000237"
     CDS             34750..35829
                     /locus_tag="JQH51_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY6074717.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="JQH51_000238"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="JQH51_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY6074718.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="JQH51_000239"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="JQH51_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY6074719.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="JQH51_000240"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="JQH51_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY6074720.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="JQH51_000241"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="JQH51_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY6074721.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="JQH51_000242"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="JQH51_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY6074722.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="JQH51_000243"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="JQH51_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY6074723.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="JQH51_000244"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="JQH51_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY6074724.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="JQH51_000245"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="JQH51_000245"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY6074725.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /locus_tag="JQH51_000246"
                     /pseudo
     CDS             43575..45245
                     /locus_tag="JQH51_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="JQH51_000247"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="JQH51_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY6074726.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="JQH51_000248"
     CDS             complement(46753..47460)
                     /locus_tag="JQH51_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY6074727.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="JQH51_000249"
     CDS             47562..48116
                     /locus_tag="JQH51_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY6074728.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="JQH51_000250"
     CDS             complement(48126..49553)
                     /locus_tag="JQH51_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY6074729.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="JQH51_000251"
     CDS             complement(49550..50257)
                     /locus_tag="JQH51_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY6074730.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="JQH51_000252"
     CDS             50421..51398
                     /locus_tag="JQH51_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY6074731.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="JQH51_000253"
     CDS             complement(51468..51950)
                     /locus_tag="JQH51_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY6074732.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="JQH51_000254"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="JQH51_000254"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY6074733.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="JQH51_000255"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="JQH51_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY6074734.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="JQH51_000256"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="JQH51_000256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY6074735.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="JQH51_000257"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="JQH51_000257"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY6074736.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="JQH51_000258"
     CDS             57061..57672
                     /locus_tag="JQH51_000258"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY6074737.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="JQH51_000259"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="JQH51_000259"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY6074738.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="JQH51_000260"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="JQH51_000260"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY6074739.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="JQH51_000261"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="JQH51_000261"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY6074740.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="JQH51_000262"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="JQH51_000262"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY6074741.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="JQH51_000263"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="JQH51_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY6074742.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="JQH51_000264"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="JQH51_000264"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY6074743.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="JQH51_000265"
     CDS             64327..64590
                     /locus_tag="JQH51_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY6074744.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="JQH51_000266"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="JQH51_000266"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY6074745.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="JQH51_000267"
     CDS             65591..66544
                     /locus_tag="JQH51_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY6074746.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="JQH51_000268"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="JQH51_000268"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY6074747.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="JQH51_000269"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="JQH51_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY6074748.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="JQH51_000270"
     CDS             complement(68151..68939)
                     /locus_tag="JQH51_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY6074749.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="JQH51_000271"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="JQH51_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY6074750.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="JQH51_000272"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="JQH51_000272"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY6074751.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /locus_tag="JQH51_000273"
     CDS             complement(69853..70413)
                     /locus_tag="JQH51_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY6074752.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="JQH51_000274"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="JQH51_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY6074753.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="JQH51_000275"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="JQH51_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="JQH51_000276"
     CDS             complement(73107..73616)
                     /locus_tag="JQH51_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6074754.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="JQH51_000277"
     CDS             73779..74024
                     /locus_tag="JQH51_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6074755.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="JQH51_000278"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="JQH51_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        18625 a        19004 c        19221 g        18826 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgagg catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac cctgggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat ggttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa tcattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat gtataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgagatagcc tgagtttaac ggacactcct
    75661 tcctgaaata gaatgg
//
LOCUS       DADLEE010000004        72934 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000004 DADLEE010000000
VERSION     DADLEE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72934
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.004"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..209)
                     /locus_tag="JQH51_000279"
     CDS             complement(<1..209)
                     /locus_tag="JQH51_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6074756.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH51_000280"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQH51_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY6074757.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH51_000281"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQH51_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY6074758.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH51_000282"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQH51_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY6074759.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH51_000283"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQH51_000283"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY6074760.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH51_000284"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQH51_000284"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY6074761.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH51_000285"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQH51_000285"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY6074762.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JQH51_000286"
     CDS             complement(4598..5320)
                     /locus_tag="JQH51_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6074763.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQH51_000287"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQH51_000287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6074764.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JQH51_000288"
     CDS             6520..6738
                     /locus_tag="JQH51_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY6074765.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JQH51_000289"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JQH51_000289"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY6074766.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JQH51_000290"
     CDS             complement(7472..7672)
                     /locus_tag="JQH51_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY6074767.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JQH51_000291"
     CDS             complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JQH51_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY6074768.1"
                     /translation="MGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFI
                     IGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGFAMSST
                     AMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWMKIGMK
                     VLEFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMALGTF
                     IAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLV
                     AVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMAPLLM
                     KLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLER
                     DISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHF
                     PHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLH
                     FRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JQH51_000292"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JQH51_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY6074769.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="JQH51_000293"
     CDS             10101..12014
                     /locus_tag="JQH51_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6074770.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="JQH51_000294"
     CDS             12014..13036
                     /locus_tag="JQH51_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6074771.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="JQH51_000295"
     CDS             13030..13248
                     /locus_tag="JQH51_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY6074772.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="JQH51_000296"
     CDS             13302..14171
                     /locus_tag="JQH51_000296"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY6074773.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="JQH51_000297"
     CDS             complement(14226..14630)
                     /locus_tag="JQH51_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY6074774.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="JQH51_000298"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="JQH51_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY6074775.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="JQH51_000299"
     CDS             15615..17705
                     /locus_tag="JQH51_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074776.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="JQH51_000300"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="JQH51_000300"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY6074777.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="JQH51_000301"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="JQH51_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY6074778.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="JQH51_000302"
     CDS             complement(19673..20275)
                     /locus_tag="JQH51_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY6074779.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="JQH51_000303"
     CDS             complement(20265..20432)
                     /locus_tag="JQH51_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY6074780.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="JQH51_000304"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="JQH51_000304"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY6074781.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="JQH51_000305"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="JQH51_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY6074782.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /locus_tag="JQH51_000306"
     CDS             22832..25375
                     /locus_tag="JQH51_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY6074783.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="JQH51_000307"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="JQH51_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY6074784.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="JQH51_000308"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="JQH51_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY6074785.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="JQH51_000309"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="JQH51_000309"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY6074786.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="JQH51_000310"
     CDS             28269..28436
                     /locus_tag="JQH51_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY6074787.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="JQH51_000311"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="JQH51_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="JQH51_000312"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="JQH51_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY6074788.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="JQH51_000313"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="JQH51_000313"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY6074789.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="JQH51_000314"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="JQH51_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY6074790.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="JQH51_000315"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="JQH51_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY6074791.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="JQH51_000316"
     CDS             complement(33764..34849)
                     /locus_tag="JQH51_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY6074792.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="JQH51_000317"
     CDS             complement(34861..36165)
                     /locus_tag="JQH51_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074793.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="JQH51_000318"
     CDS             complement(36177..36530)
                     /locus_tag="JQH51_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY6074794.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="JQH51_000319"
     CDS             complement(36541..37419)
                     /locus_tag="JQH51_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY6074795.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="JQH51_000320"
     CDS             complement(37416..38642)
                     /locus_tag="JQH51_000320"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY6074796.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="JQH51_000321"
     CDS             complement(38643..39806)
                     /locus_tag="JQH51_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY6074797.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="JQH51_000322"
     CDS             complement(39890..40252)
                     /locus_tag="JQH51_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY6074798.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="JQH51_000323"
     CDS             complement(40269..41174)
                     /locus_tag="JQH51_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074799.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="JQH51_000324"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="JQH51_000324"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY6074800.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="JQH51_000325"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="JQH51_000325"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY6074801.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="JQH51_000326"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="JQH51_000326"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY6074802.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="JQH51_000327"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="JQH51_000327"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY6074803.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="JQH51_000328"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="JQH51_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY6074804.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="JQH51_000329"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="JQH51_000329"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY6074805.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="JQH51_000330"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="JQH51_000330"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY6074806.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="JQH51_000331"
     CDS             complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="JQH51_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY6074807.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="JQH51_000332"
                     /pseudo
     CDS             complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="JQH51_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="JQH51_000333"
     CDS             complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="JQH51_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY6074808.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50254..50793)
                     /locus_tag="JQH51_000334"
     CDS             complement(50254..50793)
                     /locus_tag="JQH51_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY6074809.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="JQH51_000335"
     CDS             complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="JQH51_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY6074810.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51692..54244
                     /gene="mrcA"
                     /locus_tag="JQH51_000336"
     CDS             51692..54244
                     /gene="mrcA"
                     /locus_tag="JQH51_000336"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY6074811.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANSGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="JQH51_000337"
     CDS             complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="JQH51_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY6074812.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55289..57424
                     /gene="igaA"
                     /locus_tag="JQH51_000338"
     CDS             55289..57424
                     /gene="igaA"
                     /locus_tag="JQH51_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY6074813.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57489..58157
                     /gene="yrfG"
                     /locus_tag="JQH51_000339"
     CDS             57489..58157
                     /gene="yrfG"
                     /locus_tag="JQH51_000339"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY6074814.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58168..58569
                     /gene="hslR"
                     /locus_tag="JQH51_000340"
     CDS             58168..58569
                     /gene="hslR"
                     /locus_tag="JQH51_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY6074815.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58594..59463
                     /gene="hslO"
                     /locus_tag="JQH51_000341"
     CDS             58594..59463
                     /gene="hslO"
                     /locus_tag="JQH51_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY6074816.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59607..61358)
                     /locus_tag="JQH51_000342"
     CDS             complement(59607..61358)
                     /locus_tag="JQH51_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY6074817.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61737..63359
                     /gene="pckA"
                     /locus_tag="JQH51_000343"
     CDS             61737..63359
                     /gene="pckA"
                     /locus_tag="JQH51_000343"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY6074818.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="JQH51_000344"
     CDS             complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="JQH51_000344"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY6074819.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="JQH51_000345"
     CDS             complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="JQH51_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY6074820.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65695..66207
                     /gene="greB"
                     /locus_tag="JQH51_000346"
     CDS             65695..66207
                     /gene="greB"
                     /locus_tag="JQH51_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY6074821.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66304..68625
                     /locus_tag="JQH51_000347"
     CDS             66304..68625
                     /locus_tag="JQH51_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY6074822.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69063..69290
                     /gene="feoA"
                     /locus_tag="JQH51_000348"
     CDS             69063..69290
                     /gene="feoA"
                     /locus_tag="JQH51_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY6074823.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69307..71628
                     /gene="feoB"
                     /locus_tag="JQH51_000349"
     CDS             69307..71628
                     /gene="feoB"
                     /locus_tag="JQH51_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY6074824.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71628..71864
                     /gene="feoC"
                     /locus_tag="JQH51_000350"
     CDS             71628..71864
                     /gene="feoC"
                     /locus_tag="JQH51_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY6074825.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72067..>72934
                     /gene="rpnA"
                     /locus_tag="JQH51_000351"
     CDS             72067..>72934
                     /gene="rpnA"
                     /locus_tag="JQH51_000351"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY6074826.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17578 a        19763 c        18446 g        17147 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgccgggtaa gattagcgta
    48121 aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa
    48181 gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga
    48241 tgaaacacgt tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca
    48301 gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa
    48361 ataattgccc gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat
    48421 ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gctttttacc tcgacctgcg
    48481 tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc
    48541 ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg
    48601 gcgtgacctc catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc
    48661 cgctggaaac ctgatatgga atttcgctct cctgtttaat gctggcaggc tgaagatgtg
    48721 aggccagcag acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa
    48781 gctcaagatc cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg
    48841 ccgtcgctac ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt
    48901 gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga
    48961 caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt
    49021 caagcgcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga
    49081 cggtcatact ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt
    49141 cggcgtattg cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct
    49201 gctccgcctc ctggcgggcg atattgtcat tctgccaggc aatggaatgc actgagagaa
    49261 tgttgccttc ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct
    49321 tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca
    49381 ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca
    49441 ccatcagcgt cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta
    49501 gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt
    49561 tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag
    49621 ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt
    49681 acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg
    49741 ctaatccggc atatagatct tccggcgatt taaaagggtc acgcataccg gttaaaaggc
    49801 acaatgcaat acctgccaac aaccagcgtt taaccctcat gcggcatctc cagttgtagc
    49861 gtgaacaaaa gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc
    49921 tctgccagcc gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca
    49981 cctccctgcg cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt
    50041 ggcgaaaagg gcagcgtttt ttcctcgctg aagggagtgg tgtctaccag gcgatacagt
    50101 gcggcccact gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg
    50161 tgtgtcgatg atagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac
    50221 tggcggaggc ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt
    50281 ccttgttaac tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg
    50341 ctgattgaga tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt
    50401 aatgcttgtt gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag
    50461 ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt
    50521 aaattgtcgc tggcgctgcc gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa
    50581 acgtggcttc gttatctgta agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc
    50641 gtctatgcgc gcttcggcgc tgcctgtcag acgcagtatt agcgttatcc cgacggccag
    50701 cagcagaggc gcaacgaaca tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg
    50761 tcgccagggc aaaaaattaa ttggcggatt catcagtacg ccttcccaag cgccagcccc
    50821 agcgcgatgg caaagtctcc accaggcggc ggtagcggcg gctgacgaac agaaacggcc
    50881 tcccaggggt caaatccgcc ttcgccacat atcgcgacgc tgtcgggatc aacggataac
    50941 gctgccgcca gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag
    51001 cgcgtcgccc acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt
    51061 aaaaaaggca ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca
    51121 cgcaaccttt ctgccagcgt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg
    51181 ttataagcgg ggctgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata
    51241 tccagctcgc gggccatcgt ccctgacagc caggccgttt gctcccgctc accaagggac
    51301 atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg
    51361 tgatgacgct gcggcagttc gcgactccac ggtaacaacg ttttagccag ctgctgcgca
    51421 tcaacaatcc gcccatcttt gataatgtcg tgcgccagcg gcaaccgcca ccagcgttgc
    51481 aaaaagcatt cttttgcgcc ccgcacgatc gcaaccgctg ccgcttcttg ctgttgtaaa
    51541 tgcaaaccaa tttgccagat cttaaatgtc attgtgatga tctccttatc acccgtcact
    51601 ctgacgggta tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac
    51661 tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct
    51721 tgcagtctgt tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga
    51781 gccacaactg ccggatgtgg tgacattaaa agatgttcgc ctgcaaattc cgatgcagat
    51841 ttacagcgcc gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac
    51901 gttggatcaa atcccaccgg agatggtgaa agcctttatc gcgacggaag acagccgctt
    51961 ctacgagcat cacggcgttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt
    52021 ctccggccac gcgtcacaag gggcgagtac cattacccag cagctggcga gaaatttctt
    52081 cctcagtcca gaacgcacga tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat
    52141 tgaacagttg ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttaccttgg
    52201 ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca
    52261 actgacgctg aacgaaatgg cggtgatagc cgggttgccg aaagcgcctt ccacctttaa
    52321 tccgctctac tcaatggatc gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct
    52381 ggatgaaggg tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc
    52441 taattatcac gcgccggaga ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca
    52501 ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac
    52561 catcacccgc aaagtgcagc aggccgcgca gcaggcggta cgtaataacg tgctggacta
    52621 cgacatgcgc cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc
    52681 gtgggataac aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct
    52741 gcctgccgca gtcaccagcg ccaatcctca gggagcgacg gcgatgctgg cggacgggtc
    52801 gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca
    52861 gcagggaccg acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt
    52921 tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt
    52981 gtcgatcaat ccgcaaaatg gtgccgttat ggcgctggtc ggtggctttg atttcaatca
    53041 gagcaagttt aaccgcgcca cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt
    53101 cctctacacc gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc
    53161 aatttctcgc tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca
    53221 gtatgctggt ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt
    53281 acgtgcaatg cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt
    53341 cccggcgcaa aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcacccc
    53401 aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggc ggcttcctag tggacccgtg
    53461 gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt
    53521 agcctgcccg gaatgcgata ttccagtgat ttacggtgat acgcagaaat cgaacgtgct
    53581 ggaaaataac gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc
    53641 aatgccgcag ctggagcagg caaatcaggc gttagtggcg aagactggcg cgcaggagta
    53701 cgcaccgcac gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa
    53761 tatctttggt gaaccaggct ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg
    53821 tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg
    53881 ttacggtccg ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct
    53941 cggtcataca acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc
    54001 caagagtgcc cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga
    54061 gcagccgctg acgccgcctc cgggtattgt gacggtgaat atcgatcgca gcaccgggca
    54121 gttagctaat agtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca
    54181 acaggcggtg cacgaggtgg gaacgaccat tatcgataat ggtgaggcac aggaattgtt
    54241 ctgattaaaa aggcgcttcg gcgccttttc agttggctga caaagtgcat ttgtttatgc
    54301 cggatgcggg tgaacgcgtt atccggctaa caaaatcgtg aaaaatcaat atattgcagg
    54361 aaccccataa gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc
    54421 ctgccctttc aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa
    54481 gtcagggtct tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtagcgg
    54541 ctctggctca tcgccttcca gcgattccgg gtagagatct tgcgcgacca cgatattcat
    54601 tttgctggaa aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc
    54661 accaaatcca acctcttctt ttagctcgcg gttagcggct tcgtagacgc tttcacccgg
    54721 atcaattaat cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg
    54781 gatcaggatc aggtgatcgt ccacaatcgg cacaatcatc actgcttccc ggttggttgg
    54841 acgcattcgt tcataaacac gccgcacgcc attgctgaac tccagatcca cgctctcgac
    54901 ggtaaacagt cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga
    54961 tttgttcatc gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc
    55021 tttcggcaat gtgaattgca tgttatttac atttatgtaa cttaataaat aatcgtcctc
    55081 aaatcaaatt aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt
    55141 cagagggttt gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaagct
    55201 ccacgcttgc cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc
    55261 ctgacagact aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt
    55321 gctggcctgc tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct
    55381 gccctggacc aacgccttcg cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag
    55441 cgccgttgaa aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacgga
    55501 agctagcgcg gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct
    55561 gacacacgct atcacacgtt acggcatctc taccgacgat ccgaataaat ggcgttacta
    55621 cctcgattcg gtagaagtcc acctgccccc cttctgggaa cagtacatca acgatgagaa
    55681 taccgttgaa ctgattcata ccgattcgct gccgttggtt atttcactca acggtcatac
    55741 gctgcaggag tacatgcagg aaacgcgcgg ctatgccttg caacctgttc cgtccacgca
    55801 ggcgtcgatt cgcggggaag agagtgagca aatcgagcta ctcaatattc gcaaagaaac
    55861 gcatgaagaa tatgcgctga gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc
    55921 ctccttcctg atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc
    55981 aggcggcgca ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa
    56041 atcctccctg cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg
    56101 cgaaaacgat caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc
    56161 cgcacactgg cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta
    56221 tctcgaccgc cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa
    56281 ctttccgtta cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctagtgct
    56341 gtttatgctg ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat
    56401 gaaaggcgcg cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg
    56461 ggtaggcgat acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac
    56521 ctggagcgcg aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa
    56581 tgacgcccgc tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac
    56641 tgaagcagtt aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc
    56701 attacgttca gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt
    56761 actgaagaca gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct
    56821 ggtcaatctc ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa
    56881 gctcgatggc gtgaatgtgt tattacgccc ggtgagtgct gaatcgctgg ataacctggt
    56941 ggcaacctcc accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa
    57001 cagtccggcc cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca
    57061 gccctggcct tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca
    57121 gaaactggca caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat
    57181 cttcactgac gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg
    57241 cctgtggcgc tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta
    57301 caatggcgta caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca
    57361 ggcctattat gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga
    57421 ataacacgtt tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga
    57481 tttccctcat gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg
    57541 acggcacgtt gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa
    57601 catggggcgc gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat
    57661 atcacgacgt acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg
    57721 gtctggatat ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata
    57781 ccattccgtt tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg
    57841 ctcatccgca caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt
    57901 tattactttc cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg
    57961 tggccgaagc tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga
    58021 ttctcgatgc tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt
    58081 ccgggattgc cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga
    58141 tcccctcgct aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg
    58201 gataaatggc tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt
    58261 gaaggcggta aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg
    58321 aatgccacgc tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt
    58381 actgaacagc gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt
    58441 gtagagaaac gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac
    58501 ccggaccgac gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac
    58561 agtgaataac tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc
    58621 tatctgtttg aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa
    58681 cagatccttg agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg
    58741 gttgcgacca gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg
    58801 cagggcgacg gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc
    58861 ggtgtggcgc gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc
    58921 aatggttacg tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt
    58981 ggtctggaag gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag
    59041 ctgccgacgc gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt
    59101 atgttgttgc aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg
    59161 acgctaaccg aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg
    59221 tggcgtttgt atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa
    59281 tgcacctgct cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt
    59341 gatagcatcc tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac
    59401 tatctgttca atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg
    59461 taagttcatt aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg
    59521 cctgatgtgc tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata
    59581 cagcgtcgta tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca
    59641 tctaccgtca gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct
    59701 gtgagtaatt tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat
    59761 tcttttctgt ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg
    59821 atccgctccg cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    59881 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    59941 ggtttaacag aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt
    60001 tcctgaagat gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct
    60061 ttcataaacc cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc
    60121 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    60181 atatgactgt tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt
    60241 atcaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    60301 tgcccattac gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc
    60361 tccgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    60421 aacccccagg tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg
    60481 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    60541 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    60601 atcgcgctca ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    60661 aacggcagca accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt
    60721 gactgtgtac gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata
    60781 aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac
    60841 tcaccccaca gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat
    60901 ataagtacgt tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg
    60961 atacgaaggc tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag
    61021 ccaaaatccc aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc
    61081 agccaaccgc tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    61141 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    61201 acgccataaa caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa
    61261 accagcccct gaagtaatcc ggtagcaatc atcccctgaa gtaatccggt agcaatcatc
    61321 ccccagcgtg ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag
    61381 gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa
    61441 aagttagcgt ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat
    61501 gcgattccac tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt
    61561 tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga
    61621 gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa
    61681 cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc
    61741 gcgttaacaa tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg
    61801 atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga
    61861 caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct
    61921 tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt
    61981 tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct
    62041 ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg
    62101 acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg
    62161 cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag
    62221 gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag
    62281 aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga
    62341 ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc
    62401 tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg
    62461 atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga
    62521 aacgccgcct gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg
    62581 aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca
    62641 acgctatccg tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg
    62701 actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg
    62761 ataacatcgt taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg
    62821 ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc
    62881 acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga
    62941 cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg
    63001 cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg
    63061 gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca
    63121 tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga
    63181 tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt
    63241 ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg
    63301 ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat
    63361 gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt
    63421 cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc
    63481 agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta
    63541 tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc
    63601 gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc
    63661 ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg
    63721 atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc
    63781 gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc
    63841 tcatagccac tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc
    63901 atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac
    63961 tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca
    64021 gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc
    64081 agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta
    64141 acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca
    64201 acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc
    64261 cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa
    64321 tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt
    64381 ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc
    64441 agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc
    64501 tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg
    64561 aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac
    64621 atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag
    64681 ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat
    64741 aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt
    64801 ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat
    64861 cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg
    64921 aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat
    64981 ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt
    65041 cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct
    65101 cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac
    65161 gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca
    65221 ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt
    65281 tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat
    65341 ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag
    65401 cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc
    65461 gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag
    65521 gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat
    65581 ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct taagtataca
    65641 atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc
    65701 attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg
    65761 tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca
    65821 aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat
    65881 aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa
    65941 aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg
    66001 gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa
    66061 atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa
    66121 aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt
    66181 aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca
    66241 tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa
    66301 accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa
    66361 caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt
    66421 tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt
    66481 ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag
    66541 caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa
    66601 ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt
    66661 gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa
    66721 gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg
    66781 gatggcgcgc gctatatcct gatggaacgg tttgccgaag atgccacgct gctggcgaaa
    66841 gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa
    66901 gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct
    66961 tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg
    67021 aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg
    67081 gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg
    67141 agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg
    67201 cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg
    67261 atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg
    67321 gtaaaagtgg cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg
    67381 cacaccggac aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac
    67441 gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc
    67501 gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc
    67561 gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg
    67621 ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa
    67681 atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg
    67741 gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac
    67801 accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac
    67861 atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc
    67921 agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt
    67981 gataacccgc tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt
    68041 ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac
    68101 ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc
    68161 aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc
    68221 gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc
    68281 aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac
    68341 atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac
    68401 attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg
    68461 cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg
    68521 caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc
    68581 gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc
    68641 cctaaacctg cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat
    68701 aaaagtgccg gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc
    68761 gttaatattt atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt
    68821 aactcactat tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag
    68881 tcagattatt attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt
    68941 ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct
    69001 catgtcgcgg tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac
    69061 ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc
    69121 cggcatatcg ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg
    69181 tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat
    69241 tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata
    69301 acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc
    69361 ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc
    69421 gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc
    69481 ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt
    69541 cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt
    69601 gagcgtaacc tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg
    69661 ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg
    69721 gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg
    69781 ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca
    69841 cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg
    69901 aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac
    69961 gccggtgaag cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat
    70021 gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg
    70081 gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg
    70141 ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg
    70201 gctatcaaca tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt
    70261 gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc
    70321 ctcgcccagg gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc
    70381 atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt
    70441 gtgatggacc gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc
    70501 gtcggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt
    70561 gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc
    70621 ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg
    70681 tatatgctcg gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg
    70741 cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa
    70801 agcctgatta tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg
    70861 attatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc
    70921 gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc
    70981 aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca
    71041 atggcaaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag
    71101 gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat
    71161 gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa
    71221 gctagcaaag gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc
    71281 ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc
    71341 tcggtgatgg gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg
    71401 tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt
    71461 cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct
    71521 atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag
    71581 tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta
    71641 ttcaggtacg tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga
    71701 cattgaacac tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca
    71761 aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc
    71821 cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct
    71881 gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa
    71941 aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact
    72001 cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg
    72061 aacgccatga gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta
    72121 cgccaaccgg agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg
    72181 ctctgtgata tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt
    72241 gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat
    72301 tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac
    72361 gctattgccg caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg
    72421 ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat
    72481 tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat
    72541 gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta
    72601 attcaaaaac atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta
    72661 ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa
    72721 actggcgacg ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa
    72781 cacaaggaga gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa
    72841 gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga
    72901 tttaccgggc tgtcagaaga agagttggct gcgg
//
LOCUS       DADLEE010000005        71476 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000005 DADLEE010000000
VERSION     DADLEE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71476
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.005"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(750..2336)
                     /locus_tag="JQH51_000352"
     CDS             complement(750..2336)
                     /locus_tag="JQH51_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease glcA"
                     /protein_id="HAY6074827.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="JQH51_000353"
     CDS             complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="JQH51_000353"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY6074828.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4884..5288)
                     /locus_tag="JQH51_000354"
     CDS             complement(4884..5288)
                     /locus_tag="JQH51_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074829.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="JQH51_000355"
                     /pseudo
     CDS             complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="JQH51_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="JQH51_000356"
     CDS             complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="JQH51_000356"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY6074830.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="JQH51_000357"
                     /pseudo
     CDS             complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="JQH51_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9325..10095
                     /gene="glcC"
                     /locus_tag="JQH51_000358"
     CDS             9325..10095
                     /gene="glcC"
                     /locus_tag="JQH51_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY6074831.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10219..11360)
                     /locus_tag="JQH51_000359"
                     /pseudo
     CDS             complement(10219..11360)
                     /locus_tag="JQH51_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11722..13452
                     /locus_tag="JQH51_000360"
     CDS             11722..13452
                     /locus_tag="JQH51_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAY6074832.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13467..14363
                     /locus_tag="JQH51_000361"
     CDS             13467..14363
                     /locus_tag="JQH51_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY6074833.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            14396..14644
                     /locus_tag="JQH51_000362"
     CDS             14396..14644
                     /locus_tag="JQH51_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY6074834.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14644..15815
                     /locus_tag="JQH51_000363"
                     /pseudo
     CDS             14644..15815
                     /locus_tag="JQH51_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="JQH51_000364"
     CDS             complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="JQH51_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY6074835.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="JQH51_000365"
     CDS             complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="JQH51_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY6074836.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18028..18588)
                     /locus_tag="JQH51_000366"
     CDS             complement(18028..18588)
                     /locus_tag="JQH51_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY6074837.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18600..19424)
                     /locus_tag="JQH51_000367"
     CDS             complement(18600..19424)
                     /locus_tag="JQH51_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074838.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19424..20773)
                     /locus_tag="JQH51_000368"
     CDS             complement(19424..20773)
                     /locus_tag="JQH51_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY6074839.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20819..21577)
                     /locus_tag="JQH51_000369"
     CDS             complement(20819..21577)
                     /locus_tag="JQH51_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074840.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21609..22322)
                     /locus_tag="JQH51_000370"
     CDS             complement(21609..22322)
                     /locus_tag="JQH51_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY6074841.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22496..23188
                     /locus_tag="JQH51_000371"
     CDS             22496..23188
                     /locus_tag="JQH51_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY6074842.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="JQH51_000372"
     CDS             complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="JQH51_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY6074843.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25028..26887)
                     /gene="gss"
                     /locus_tag="JQH51_000373"
     CDS             complement(25028..26887)
                     /gene="gss"
                     /locus_tag="JQH51_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY6074844.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27092..27958
                     /gene="yghU"
                     /locus_tag="JQH51_000374"
     CDS             27092..27958
                     /gene="yghU"
                     /locus_tag="JQH51_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY6074845.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="JQH51_000375"
     CDS             complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="JQH51_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY6074846.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="JQH51_000376"
     CDS             complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="JQH51_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY6074847.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="JQH51_000377"
     CDS             complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="JQH51_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY6074848.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29278..29772)
                     /locus_tag="JQH51_000378"
     CDS             complement(29278..29772)
                     /locus_tag="JQH51_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY6074849.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="JQH51_000379"
     CDS             complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="JQH51_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY6074850.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="JQH51_000380"
     CDS             complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="JQH51_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY6074851.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="JQH51_000381"
     CDS             complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="JQH51_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY6074852.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="JQH51_000382"
     CDS             complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="JQH51_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY6074853.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34936..35223)
                     /locus_tag="JQH51_000383"
     CDS             complement(34936..35223)
                     /locus_tag="JQH51_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY6074854.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35342..36268)
                     /locus_tag="JQH51_000384"
     CDS             complement(35342..36268)
                     /locus_tag="JQH51_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY6074855.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            36386..37426
                     /gene="gpr"
                     /locus_tag="JQH51_000385"
     CDS             36386..37426
                     /gene="gpr"
                     /locus_tag="JQH51_000385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY6074856.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37466..37960)
                     /locus_tag="JQH51_000386"
     CDS             complement(37466..37960)
                     /locus_tag="JQH51_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY6074857.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38151..39035
                     /gene="yghA"
                     /locus_tag="JQH51_000387"
     CDS             38151..39035
                     /gene="yghA"
                     /locus_tag="JQH51_000387"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY6074858.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="JQH51_000388"
     CDS             complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="JQH51_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY6074859.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="JQH51_000389"
     CDS             complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="JQH51_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY6074860.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40725..41912
                     /gene="metC"
                     /locus_tag="JQH51_000390"
     CDS             40725..41912
                     /gene="metC"
                     /locus_tag="JQH51_000390"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY6074861.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42052..42711
                     /locus_tag="JQH51_000391"
     CDS             42052..42711
                     /locus_tag="JQH51_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6074862.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42751..43650)
                     /locus_tag="JQH51_000392"
     CDS             complement(42751..43650)
                     /locus_tag="JQH51_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6074863.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43844..45007
                     /gene="yqhD"
                     /locus_tag="JQH51_000393"
     CDS             43844..45007
                     /gene="yqhD"
                     /locus_tag="JQH51_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY6074864.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45112..45939
                     /gene="dkgA"
                     /locus_tag="JQH51_000394"
     CDS             45112..45939
                     /gene="dkgA"
                     /locus_tag="JQH51_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY6074865.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46139..47065
                     /locus_tag="JQH51_000395"
     CDS             46139..47065
                     /locus_tag="JQH51_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY6074866.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47116..47373
                     /gene="yqhH"
                     /locus_tag="JQH51_000396"
     CDS             47116..47373
                     /gene="yqhH"
                     /locus_tag="JQH51_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY6074867.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47416..49635)
                     /locus_tag="JQH51_000397"
     CDS             complement(47416..49635)
                     /locus_tag="JQH51_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY6074868.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="JQH51_000398"
     CDS             complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="JQH51_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY6074869.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="JQH51_000399"
                     /gene_synonym="parF"
     CDS             complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="JQH51_000399"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY6074870.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52204..54462)
                     /gene="parC"
                     /locus_tag="JQH51_000400"
     CDS             complement(52204..54462)
                     /gene="parC"
                     /locus_tag="JQH51_000400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY6074871.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55008..55490)
                     /locus_tag="JQH51_000401"
     CDS             complement(55008..55490)
                     /locus_tag="JQH51_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6074872.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="JQH51_000402"
     CDS             complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="JQH51_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY6074873.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56087..56746
                     /gene="qseB"
                     /locus_tag="JQH51_000403"
     CDS             56087..56746
                     /gene="qseB"
                     /locus_tag="JQH51_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY6074874.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56743..58017
                     /gene="qseC"
                     /locus_tag="JQH51_000404"
     CDS             56743..58017
                     /gene="qseC"
                     /locus_tag="JQH51_000404"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY6074875.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58063..58395)
                     /locus_tag="JQH51_000405"
     CDS             complement(58063..58395)
                     /locus_tag="JQH51_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY6074876.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58714..59295
                     /gene="mdaB"
                     /locus_tag="JQH51_000406"
     CDS             58714..59295
                     /gene="mdaB"
                     /locus_tag="JQH51_000406"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY6074877.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59326..59640
                     /locus_tag="JQH51_000407"
     CDS             59326..59640
                     /locus_tag="JQH51_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY6074878.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59688..61580)
                     /gene="parE"
                     /locus_tag="JQH51_000408"
     CDS             complement(59688..61580)
                     /gene="parE"
                     /locus_tag="JQH51_000408"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY6074879.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="JQH51_000409"
     CDS             complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="JQH51_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY6074880.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="JQH51_000410"
     CDS             complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="JQH51_000410"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY6074881.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63042..63464)
                     /locus_tag="JQH51_000411"
     CDS             complement(63042..63464)
                     /locus_tag="JQH51_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY6074882.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="JQH51_000412"
     CDS             complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="JQH51_000412"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY6074883.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64299..65780
                     /gene="tolC"
                     /locus_tag="JQH51_000413"
     CDS             64299..65780
                     /gene="tolC"
                     /locus_tag="JQH51_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY6074884.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65928..66599
                     /locus_tag="JQH51_000414"
     CDS             65928..66599
                     /locus_tag="JQH51_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY6074885.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66605..67765
                     /locus_tag="JQH51_000415"
     CDS             66605..67765
                     /locus_tag="JQH51_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY6074886.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67803..68618)
                     /gene="ygiD"
                     /locus_tag="JQH51_000416"
     CDS             complement(67803..68618)
                     /gene="ygiD"
                     /locus_tag="JQH51_000416"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY6074887.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68734..69507
                     /gene="zupT"
                     /locus_tag="JQH51_000417"
     CDS             68734..69507
                     /gene="zupT"
                     /locus_tag="JQH51_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY6074888.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69565..69735)
                     /locus_tag="JQH51_000418"
     CDS             complement(69565..69735)
                     /locus_tag="JQH51_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074889.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="JQH51_000419"
     CDS             complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="JQH51_000419"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY6074890.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70754..70902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            70964..71314
                     /gene="ubiK"
                     /locus_tag="JQH51_000420"
     CDS             70964..71314
                     /gene="ubiK"
                     /locus_tag="JQH51_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY6074891.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT        17836 a        19053 c        17654 g        16933 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac
      841 agttcagatt ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg
      901 gagatcatct tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag
      961 acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg
     1021 tccgacccgg taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc
     1081 acgcctgtac ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg
     1141 aaggcgaatg ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt
     1201 tcggcaaaga cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc
     1261 gccgcaataa aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc
     1321 attggcgttg gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg
     1381 atctggaaat taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac
     1441 ggcttcatgg tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga
     1501 atgatttgcc ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc
     1561 accatcgcac ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag
     1621 actttaagga ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt
     1681 tccggaccaa tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc
     1741 agcgccgctg gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag
     1801 aacggcacaa gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac
     1861 ggatcgattc ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc
     1921 accgtcgcac caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt
     1981 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     2041 tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt
     2101 ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt
     2161 ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt
     2221 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     2281 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     2341 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     2401 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2461 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2521 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2581 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2641 ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt
     2701 tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc
     2761 acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc
     2821 gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc
     2881 tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata
     2941 tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc
     3001 ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc
     3061 tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc
     3121 accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg
     3181 gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta
     3241 gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc
     3301 tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct
     3361 ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc
     3421 cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc
     3481 atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc
     3541 tgagcgatac agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt
     3601 ttcagggtat tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg
     3661 aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc
     3721 gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga
     3781 ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga
     3841 cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg
     3901 gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt
     3961 ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct
     4021 tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg
     4081 acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt
     4141 tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg
     4201 ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac
     4261 tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt
     4321 tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc
     4381 tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac
     4441 gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt
     4501 gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt
     4561 tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta
     4621 taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc
     4681 tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc
     4741 tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca
     4801 tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc
     4861 attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc
     4921 tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca
     4981 acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta
     5041 cgtccgttgt tcaccatctc ttcatagccc ttagttgcac gacgccccag cgcggcggta
     5101 cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac
     5161 gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc
     5221 gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta
     5281 gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac
     5341 agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat
     5401 ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc
     5461 gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc
     5521 cgttaaggta aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc
     5581 atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt
     5641 ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg
     5701 cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc
     5761 gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca
     5821 ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc
     5881 caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag
     5941 cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag
     6001 cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc
     6061 aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt
     6121 cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg
     6181 ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt
     6241 gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc
     6301 gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata
     6361 gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc
     6421 ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc
     6481 tgccgcatct cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat
     6541 gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg
     6601 gaataaagga gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg
     6661 accgccagcg ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca
     6721 gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc
     6781 gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc
     6841 acgcaattgc tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc
     6901 ttttaccgat ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa
     6961 gccactaatg ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag
     7021 gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat
     7081 ttcagtgagc acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc
     7141 ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat
     7201 gcgcgtgccg aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag
     7261 cccgcaggcg accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg
     7321 aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg
     7381 agcggttatc accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc
     7441 cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac
     7501 cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc
     7561 acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca
     7621 tgcatggcac caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc
     7681 aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg
     7741 aactgggcgc acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg
     7801 ccgccaactt caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat
     7861 tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga
     7921 aagacgttgg caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg
     7981 ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt
     8041 cctaccgccg ggaacgcatt tttgagaccg gcccagaaac gtacgcgctc tgcttcgtcc
     8101 tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag
     8161 tcttcctgta cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg
     8221 acgggataac cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc
     8281 tccagcccgc cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt
     8341 tctaccgagt caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt
     8401 accgtcactt cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg
     8461 tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt
     8521 tgcacttcaa ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca
     8581 ccggcatttt ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc
     8641 gcgtaataga gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc
     8701 tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc
     8761 accaacaaca cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca
     8821 cgggtcacca ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc
     8881 atttgcttag gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac
     8941 tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc
     9001 agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg
     9061 tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc
     9121 gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc
     9181 ggtcagacca ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg
     9241 atgtaacata atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt
     9301 cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt
     9361 gcagagagta tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc
     9421 tcggaacgtc gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg
     9481 accgtgctgc gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca
     9541 cggcttaatc gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga
     9601 acgctgtacg atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg
     9661 gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc
     9721 gccgccagtg agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct
     9781 ttccatctcg ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca
     9841 ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca
     9901 cagcaaaaaa agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg
     9961 ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc
    10021 cacgatatcg agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac
    10081 aaggtgggaa tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac
    10141 ttaacaggat ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc
    10201 gctaaacagg atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc
    10261 cgccccaatg cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat
    10321 ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag
    10381 cgcaatcacc gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac
    10441 tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg
    10501 aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac
    10561 gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta
    10621 agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc
    10681 tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga
    10741 ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga
    10801 gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa
    10861 cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt
    10921 ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc
    10981 ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg
    11041 gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg
    11101 taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct
    11161 ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa
    11221 tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat
    11281 gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc
    11341 atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt
    11401 tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat
    11461 agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt
    11521 gtcaatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt
    11581 cataataaaa acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat
    11641 gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta
    11701 tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta
    11761 cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt
    11821 agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag
    11881 acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa
    11941 ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc
    12001 tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg
    12061 gattcctgga gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc
    12121 actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat
    12181 gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca
    12241 gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc
    12301 gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt
    12361 aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg
    12421 gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag
    12481 gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt
    12541 tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc
    12601 gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca
    12661 gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc
    12721 atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc
    12781 ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg
    12841 ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa
    12901 catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat
    12961 atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac
    13021 gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat
    13081 ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg
    13141 caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct
    13201 tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa
    13261 cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc
    13321 gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc
    13381 cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa
    13441 tccctggcat aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata
    13501 ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg
    13561 ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta
    13621 gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca
    13681 aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa
    13741 aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg
    13801 agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg
    13861 acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt
    13921 taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata
    13981 cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg
    14041 ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact
    14101 ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca
    14161 ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag
    14221 gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg
    14281 caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg
    14341 cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt
    14401 aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg
    14461 tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt
    14521 gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct
    14581 gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa
    14641 ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac
    14701 aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg
    14761 atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat
    14821 catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt
    14881 tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag
    14941 tttttcaatc gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca
    15001 tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta
    15061 tttatgacgt ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta
    15121 aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg
    15181 ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca
    15241 aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg
    15301 caacggggcg cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg
    15361 gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg
    15421 aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca
    15481 ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc
    15541 tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt
    15601 cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct
    15661 ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg
    15721 caggcatcac gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg
    15781 ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc
    15841 cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa
    15901 acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat
    15961 taaccatgat ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac
    16021 ggctacccat cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca
    16081 tcgccagggt caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc
    16141 tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag
    16201 tattccccgg cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa
    16261 gattatctaa tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat
    16321 gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa
    16381 gcgagagatc gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca
    16441 gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt
    16501 catcgtcctc ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg
    16561 gcgacgccac ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca
    16621 gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac
    16681 tgtttttcga taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa
    16741 gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat
    16801 aaccgcccgg gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca
    16861 gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga
    16921 aaacattcat cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag
    16981 gcccattagc atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt
    17041 agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag
    17101 tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag
    17161 cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc
    17221 ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg
    17281 gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc
    17341 acgcccctga tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc
    17401 gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt
    17461 agaagtataa atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag
    17521 gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag
    17581 ctccgactgc gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata
    17641 catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta
    17701 caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat
    17761 ctgccctagc gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc
    17821 aatcagtact ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc
    17881 cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac
    17941 gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc
    18001 actaacgccc tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat
    18061 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc
    18121 tttattcagc accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg
    18181 tccattgccg tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt
    18241 gccaacaaca aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg
    18301 ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt
    18361 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg
    18421 tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc
    18481 ttcctggata taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
    18541 cggggctaag agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc
    18601 tatagcggaa aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc
    18661 aacggtgcat aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
    18721 tctcccatca ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt
    18781 tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
    18841 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc
    18901 atttgctttt gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat
    18961 gaatagttca ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa
    19021 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg
    19081 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
    19141 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
    19201 tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg
    19261 agccagaatt gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt
    19321 gcaatagtcg gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca
    19381 aatagacgct cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg
    19441 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga
    19501 gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
    19561 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
    19621 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg
    19681 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
    19741 caaacaccag cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac
    19801 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
    19861 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
    19921 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
    19981 tccccaccaa cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
    20041 ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt
    20101 tgaaggcgtt atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg
    20161 tgccgccaac cagattcgac acgtaccaag caataacaaa acccacaaaa ccaaagtcaa
    20221 aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
    20281 caatcaaatc aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
    20341 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
    20401 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
    20461 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
    20521 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
    20581 tcgacttggc gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt
    20641 taccggcaca cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta
    20701 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
    20761 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt
    20821 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt
    20881 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
    20941 caatcgtcga catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc
    21001 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
    21061 ccattttttg gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
    21121 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
    21181 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
    21241 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
    21301 ttttcatgct ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa
    21361 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc
    21421 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac
    21481 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
    21541 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
    21601 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg
    21661 gctggttgcc gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta
    21721 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
    21781 tgttcatcaa tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac
    21841 agcttcaact cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    21901 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    21961 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    22021 gaaagcagat agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt
    22081 acatgtgccg ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt
    22141 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    22201 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    22261 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    22321 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    22381 gttcaacctt attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa
    22441 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
    22501 gtcaataaca cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt
    22561 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    22621 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt
    22681 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc
    22741 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
    22801 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
    22861 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
    22921 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
    22981 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
    23041 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
    23101 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
    23161 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    23221 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
    23281 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    23341 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    23401 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    23461 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    23521 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
    23581 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    23641 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc
    23701 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    23761 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    23821 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
    23881 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg
    23941 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag
    24001 gggcaatccc caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc
    24061 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
    24121 gtttacgttt gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    24181 ttttcgtccc actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    24241 cgacgatagg ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg
    24301 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    24361 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    24421 taatcgccgc cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca
    24481 gcaacaaatc gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata
    24541 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac
    24601 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    24661 agaacaacac aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa
    24721 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    24781 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
    24841 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
    24901 gtattccact ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat
    24961 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
    25021 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
    25081 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
    25141 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
    25201 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
    25261 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
    25321 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
    25381 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
    25441 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
    25501 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
    25561 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
    25621 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
    25681 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
    25741 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
    25801 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
    25861 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
    25921 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
    25981 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
    26041 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
    26101 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
    26161 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
    26221 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
    26281 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
    26341 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
    26401 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
    26461 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
    26521 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
    26581 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
    26641 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
    26701 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
    26761 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
    26821 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
    26881 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
    26941 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
    27001 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
    27061 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
    27121 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
    27181 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
    27241 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
    27301 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
    27361 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
    27421 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
    27481 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
    27541 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
    27601 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
    27661 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
    27721 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
    27781 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
    27841 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
    27901 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
    27961 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
    28021 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
    28081 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
    28141 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
    28201 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
    28261 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
    28321 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
    28381 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
    28441 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
    28501 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
    28561 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
    28621 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
    28681 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
    28741 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
    28801 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
    28861 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
    28921 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
    28981 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
    29041 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
    29101 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
    29161 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
    29221 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
    29281 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
    29341 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
    29401 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
    29461 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
    29521 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
    29581 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    29641 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    29701 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    29761 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    29821 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    29881 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    29941 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
    30001 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    30061 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
    30121 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
    30181 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
    30241 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    30301 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    30361 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    30421 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
    30481 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    30541 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
    30601 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    30661 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
    30721 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
    30781 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
    30841 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    30901 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    30961 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    31021 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    31081 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    31141 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    31201 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    31261 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    31321 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    31381 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    31441 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    31501 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    31561 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    31621 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
    31681 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
    31741 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    31801 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    31861 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
    31921 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
    31981 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    32041 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
    32101 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
    32161 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
    32221 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    32281 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    32341 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    32401 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    32461 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    32521 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    32581 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    32641 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    32701 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    32761 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    32821 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    32881 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    32941 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    33001 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    33061 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
    33121 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    33181 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    33241 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    33301 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    33361 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    33421 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    33481 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    33541 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    33601 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    33661 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    33721 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    33781 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    33841 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
    33901 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    33961 agccgaggtg gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa
    34021 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    34081 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    34141 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    34201 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
    34261 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
    34321 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
    34381 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
    34441 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
    34501 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    34561 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    34621 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    34681 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    34741 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
    34801 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    34861 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    34921 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    34981 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    35041 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    35101 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    35161 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    35221 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    35281 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    35341 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    35401 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    35461 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    35521 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    35581 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    35641 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    35701 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    35761 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    35821 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    35881 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    35941 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    36001 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    36061 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    36121 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    36181 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    36241 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    36301 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    36361 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    36421 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    36481 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    36541 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    36601 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    36661 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    36721 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    36781 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    36841 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    36901 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    36961 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    37021 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    37081 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    37141 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    37201 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    37261 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    37321 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    37381 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    37441 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    37501 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    37561 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    37621 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    37681 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    37741 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    37801 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    37861 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    37921 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    37981 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    38041 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    38101 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    38161 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    38221 caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    38281 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    38341 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    38401 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    38461 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    38521 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    38581 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    38641 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    38701 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    38761 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    38821 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    38881 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    38941 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    39001 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    39061 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    39121 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    39181 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    39241 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    39301 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    39361 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    39421 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    39481 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    39541 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    39601 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    39661 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    39721 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    39781 agcggcgctg gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac
    39841 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    39901 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    39961 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    40021 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    40081 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    40141 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    40201 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    40261 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    40321 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    40381 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    40441 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    40501 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    40561 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    40621 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    40681 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    40741 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    40801 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    40861 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    40921 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    40981 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    41041 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    41101 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    41161 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    41221 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    41281 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    41341 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    41401 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    41461 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    41521 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    41581 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    41641 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    41701 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    41761 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    41821 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    41881 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    41941 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    42001 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    42061 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    42121 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    42181 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    42241 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    42301 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    42361 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    42421 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    42481 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    42541 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    42601 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    42661 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    42721 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    42781 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    42841 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    42901 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    42961 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    43021 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    43081 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    43141 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    43201 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    43261 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    43321 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    43381 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    43441 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    43501 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    43561 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    43621 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    43681 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    43741 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    43801 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    43861 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    43921 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    43981 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    44041 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    44101 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    44161 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    44221 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    44281 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    44341 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    44401 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    44461 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    44521 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    44581 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    44641 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    44701 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    44761 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    44821 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    44881 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    44941 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    45001 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    45061 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    45121 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    45181 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    45241 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    45301 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    45361 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    45421 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    45481 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    45541 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    45601 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    45661 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    45721 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    45781 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    45841 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    45901 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    45961 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    46021 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    46081 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    46141 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    46201 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    46261 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    46321 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    46381 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    46441 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    46501 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    46561 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    46621 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    46681 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    46741 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    46801 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    46861 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    46921 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    46981 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    47041 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    47101 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    47161 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    47221 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    47281 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    47341 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    47401 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    47461 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    47521 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    47581 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    47641 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    47701 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    47761 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    47821 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    47881 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    47941 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    48001 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    48061 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    48121 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    48181 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    48241 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    48301 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    48361 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    48421 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    48481 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    48541 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    48601 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    48661 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    48721 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    48781 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    48841 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    48901 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    48961 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    49021 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    49081 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    49141 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    49201 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    49261 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    49321 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    49381 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    49441 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    49501 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    49561 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    49621 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    49681 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    49741 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    49801 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    49861 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    49921 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    49981 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    50041 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    50101 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    50161 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    50221 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    50281 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    50341 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    50401 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    50461 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    50521 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    50581 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    50641 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    50701 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    50761 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    50821 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    50881 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    50941 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    51001 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    51061 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    51121 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    51181 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    51241 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    51301 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    51361 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    51421 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    51481 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    51541 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    51601 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    51661 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    51721 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    51781 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    51841 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    51901 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    51961 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    52021 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    52081 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    52141 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    52201 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    52261 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    52321 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    52381 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    52441 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    52501 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    52561 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    52621 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    52681 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    52741 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    52801 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    52861 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    52921 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    52981 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    53041 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    53101 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    53161 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    53221 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    53281 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    53341 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    53401 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    53461 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    53521 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    53581 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    53641 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    53701 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    53761 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    53821 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    53881 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    53941 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    54001 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    54061 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    54121 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    54181 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgatatcgc cgtgcggatg
    54241 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    54301 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    54361 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    54421 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    54481 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    54541 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    54601 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    54661 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    54721 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    54781 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    54841 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    54901 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    54961 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    55021 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    55081 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    55141 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    55201 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg
    55261 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    55321 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    55381 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    55441 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    55501 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    55561 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    55621 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    55681 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    55741 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    55801 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    55861 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    55921 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    55981 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    56041 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    56101 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    56161 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    56221 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    56281 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    56341 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    56401 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    56461 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    56521 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    56581 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    56641 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    56701 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    56761 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    56821 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    56881 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    56941 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    57001 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    57061 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    57121 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    57181 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    57241 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    57301 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    57361 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    57421 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    57481 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    57541 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    57601 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    57661 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    57721 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    57781 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    57841 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    57901 gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    57961 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    58021 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    58081 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    58141 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    58201 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    58261 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    58321 agaatctatt aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat
    58381 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    58441 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    58501 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    58561 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    58621 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    58681 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    58741 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    58801 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    58861 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    58921 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    58981 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    59041 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    59101 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    59161 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    59221 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    59281 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    59341 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    59401 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    59461 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    59521 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    59581 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    59641 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    59701 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    59761 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    59821 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    59881 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    59941 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    60001 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    60061 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    60121 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    60181 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    60241 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    60301 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    60361 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    60421 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    60481 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    60541 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    60601 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    60661 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    60721 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    60781 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    60841 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    60901 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    60961 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    61021 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    61081 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    61141 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    61201 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    61261 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    61321 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    61381 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    61441 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    61501 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    61561 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    61621 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    61681 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    61741 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    61801 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    61861 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    61921 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    61981 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    62041 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    62101 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    62161 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    62221 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    62281 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    62341 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    62401 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    62461 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    62521 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    62581 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    62641 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    62701 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    62761 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    62821 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    62881 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    62941 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    63001 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    63061 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    63121 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    63181 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    63241 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    63301 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    63361 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    63421 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    63481 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    63541 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    63601 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    63661 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    63721 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    63781 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    63841 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    63901 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    63961 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    64021 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    64081 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    64141 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    64201 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    64261 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    64321 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    64381 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    64441 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    64501 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    64561 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    64621 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    64681 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    64741 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    64801 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    64861 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    64921 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    64981 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    65041 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    65101 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    65161 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    65221 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    65281 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    65341 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    65401 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    65461 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    65521 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    65581 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    65641 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    65701 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    65761 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    65821 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    65881 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    65941 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    66001 ctctcgcggt tgccactgtt cttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    66061 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    66121 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    66181 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    66241 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    66301 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    66361 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    66421 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    66481 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    66541 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    66601 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    66661 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    66721 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    66781 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    66841 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    66901 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    66961 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    67021 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    67081 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    67141 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    67201 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    67261 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    67321 gctgcgcgaa atgttctcca ccaagctgga agatgctggc gtacgctggc tggaaccggc
    67381 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    67441 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    67501 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    67561 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    67621 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    67681 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    67741 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    67801 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    67861 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    67921 tctggcgttg ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    67981 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    68041 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    68101 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    68161 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    68221 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    68281 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    68341 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    68401 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    68461 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    68521 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    68581 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    68641 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    68701 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    68761 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    68821 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    68881 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    68941 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    69001 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    69061 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    69121 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    69181 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    69241 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    69301 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    69361 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    69421 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    69481 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    69541 acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact
    69601 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    69661 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    69721 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    69781 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    69841 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    69901 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    69961 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    70021 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    70081 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    70141 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    70201 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    70261 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    70321 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    70381 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    70441 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    70501 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    70561 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    70621 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa cctcactaaa attatggtta
    70681 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
    70741 tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
    70801 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
    70861 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
    70921 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
    70981 cactacaatg gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
    71041 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg
    71101 ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct
    71161 tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact
    71221 ggcgctgctt gaacaaagaa tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca
    71281 gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat
    71341 tatgaattaa tcctctaatt tacagtgttt tctattacat tatcgcttaa tcccattcag
    71401 agtatcatca caaaaatgaa tgattgcaat tgaaaatata attgttatgt attctttaag
    71461 ttttacttaa tcgata
//
LOCUS       DADLEE010000006        70712 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000006 DADLEE010000000
VERSION     DADLEE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.006"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..795
                     /locus_tag="JQH51_000421"
     CDS             <1..795
                     /locus_tag="JQH51_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6074892.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="JQH51_000422"
     CDS             806..1897
                     /locus_tag="JQH51_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6074893.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="JQH51_000423"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="JQH51_000423"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY6074894.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /locus_tag="JQH51_000424"
     CDS             2865..4901
                     /locus_tag="JQH51_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY6074895.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="JQH51_000425"
     CDS             4859..5254
                     /locus_tag="JQH51_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY6074896.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="JQH51_000426"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="JQH51_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY6074897.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="JQH51_000427"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="JQH51_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY6074898.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="JQH51_000428"
     CDS             complement(6563..7603)
                     /locus_tag="JQH51_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY6074899.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="JQH51_000429"
     CDS             complement(7676..8311)
                     /locus_tag="JQH51_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY6074900.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="JQH51_000430"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="JQH51_000430"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY6074901.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="JQH51_000431"
     CDS             complement(8937..9380)
                     /locus_tag="JQH51_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY6074902.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="JQH51_000432"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="JQH51_000432"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY6074903.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="JQH51_000433"
     CDS             complement(9720..10223)
                     /locus_tag="JQH51_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6074904.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="JQH51_000434"
     CDS             complement(10217..10741)
                     /locus_tag="JQH51_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY6074905.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="JQH51_000435"
     CDS             10950..11945
                     /locus_tag="JQH51_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY6074906.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="JQH51_000436"
     CDS             11954..12832
                     /locus_tag="JQH51_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY6074907.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="JQH51_000437"
     CDS             12913..13920
                     /locus_tag="JQH51_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY6074908.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="JQH51_000438"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="JQH51_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY6074909.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /locus_tag="JQH51_000439"
     CDS             complement(15436..17325)
                     /locus_tag="JQH51_000439"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY6074910.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="JQH51_000440"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="JQH51_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY6074911.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="JQH51_000441"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="JQH51_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY6074912.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="JQH51_000442"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="JQH51_000442"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY6074913.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="JQH51_000443"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="JQH51_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY6074914.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="JQH51_000444"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="JQH51_000444"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY6074915.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="JQH51_000445"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="JQH51_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY6074916.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="JQH51_000446"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="JQH51_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY6074917.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="JQH51_000447"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="JQH51_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY6074918.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="JQH51_000448"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="JQH51_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY6074919.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="JQH51_000449"
     tRNA            complement(27654..27730)
                     /locus_tag="JQH51_000449"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="JQH51_000450"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="JQH51_000450"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY6074920.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="JQH51_000451"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="JQH51_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="JQH51_000452"
     tRNA            complement(31514..31600)
                     /locus_tag="JQH51_000452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="JQH51_000453"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="JQH51_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY6074921.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="JQH51_000454"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="JQH51_000454"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY6074922.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="JQH51_000455"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="JQH51_000455"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY6074923.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="JQH51_000456"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="JQH51_000456"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY6074924.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="JQH51_000457"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="JQH51_000457"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY6074925.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="JQH51_000458"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="JQH51_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY6074926.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="JQH51_000459"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="JQH51_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY6074927.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="JQH51_000460"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="JQH51_000460"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY6074928.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="JQH51_000461"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="JQH51_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY6074929.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="JQH51_000462"
     CDS             complement(41212..42177)
                     /locus_tag="JQH51_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6074930.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="JQH51_000463"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="JQH51_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY6074931.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="JQH51_000464"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="JQH51_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY6074932.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="JQH51_000465"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="JQH51_000465"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY6074933.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="JQH51_000466"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="JQH51_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY6074934.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="JQH51_000467"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="JQH51_000467"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY6074935.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="JQH51_000468"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="JQH51_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY6074936.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="JQH51_000469"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="JQH51_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY6074937.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="JQH51_000470"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="JQH51_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY6074938.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="JQH51_000471"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="JQH51_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY6074939.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="JQH51_000472"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="JQH51_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY6074940.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="JQH51_000473"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="JQH51_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY6074941.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="JQH51_000474"
     CDS             49715..50680
                     /locus_tag="JQH51_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY6074942.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="JQH51_000475"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="JQH51_000475"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY6074943.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="JQH51_000476"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="JQH51_000476"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY6074944.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="JQH51_000477"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="JQH51_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY6074945.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="JQH51_000478"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="JQH51_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY6074946.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="JQH51_000479"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="JQH51_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY6074947.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="JQH51_000480"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="JQH51_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY6074948.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="JQH51_000481"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="JQH51_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY6074949.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="JQH51_000482"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="JQH51_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY6074950.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="JQH51_000483"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="JQH51_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY6074951.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="JQH51_000484"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="JQH51_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY6074952.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="JQH51_000485"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="JQH51_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY6074953.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="JQH51_000486"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="JQH51_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="JQH51_000487"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="JQH51_000487"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY6074954.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="JQH51_000488"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="JQH51_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY6074955.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="JQH51_000489"
     CDS             complement(62547..63476)
                     /locus_tag="JQH51_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY6074956.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="JQH51_000490"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="JQH51_000490"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY6074957.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /locus_tag="JQH51_000491"
     CDS             68624..70042
                     /locus_tag="JQH51_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6074958.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70705
                     /locus_tag="JQH51_000492"
     CDS             70226..70705
                     /locus_tag="JQH51_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY6074959.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19176 c        17560 g        16662 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DADLEE010000007        67654 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000007 DADLEE010000000
VERSION     DADLEE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67654
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.007"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..228
                     /locus_tag="JQH51_000493"
     CDS             <1..228
                     /locus_tag="JQH51_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6074960.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            623..1262
                     /locus_tag="JQH51_000494"
                     /pseudo
     CDS             623..1262
                     /locus_tag="JQH51_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1365..1688
                     /locus_tag="JQH51_000495"
     CDS             1365..1688
                     /locus_tag="JQH51_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HAY6074961.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="JQH51_000496"
     CDS             complement(1755..3212)
                     /gene="nuoN"
                     /locus_tag="JQH51_000496"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY6074962.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="JQH51_000497"
     CDS             complement(3219..4748)
                     /gene="nuoM"
                     /locus_tag="JQH51_000497"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY6074963.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="JQH51_000498"
     CDS             complement(4912..6753)
                     /gene="nuoL"
                     /locus_tag="JQH51_000498"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY6074964.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="JQH51_000499"
     CDS             complement(6750..7052)
                     /gene="nuoK"
                     /locus_tag="JQH51_000499"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY6074965.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="JQH51_000500"
     CDS             complement(7049..7603)
                     /gene="nuoJ"
                     /locus_tag="JQH51_000500"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY6074966.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="JQH51_000501"
     CDS             complement(7615..8157)
                     /gene="nuoI"
                     /locus_tag="JQH51_000501"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY6074967.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="JQH51_000502"
     CDS             complement(8172..9149)
                     /gene="nuoH"
                     /locus_tag="JQH51_000502"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY6074968.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="JQH51_000503"
     CDS             complement(9146..11872)
                     /gene="nuoG"
                     /locus_tag="JQH51_000503"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY6074969.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="JQH51_000504"
     CDS             complement(11925..13262)
                     /gene="nuoF"
                     /locus_tag="JQH51_000504"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY6074970.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="JQH51_000505"
     CDS             complement(13259..13759)
                     /gene="nuoE"
                     /locus_tag="JQH51_000505"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY6074971.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="JQH51_000506"
     CDS             complement(13762..15564)
                     /gene="nuoC"
                     /locus_tag="JQH51_000506"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY6074972.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15658..16320)
                     /locus_tag="JQH51_000507"
     CDS             complement(15658..16320)
                     /locus_tag="JQH51_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY6074973.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="JQH51_000508"
     CDS             complement(16336..16779)
                     /gene="nuoA"
                     /locus_tag="JQH51_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY6074974.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="JQH51_000509"
     CDS             complement(17410..18348)
                     /gene="lrhA"
                     /locus_tag="JQH51_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY6074975.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19267..20484
                     /locus_tag="JQH51_000510"
     CDS             19267..20484
                     /locus_tag="JQH51_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY6074976.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20568..21167
                     /gene="yfbR"
                     /locus_tag="JQH51_000511"
     CDS             20568..21167
                     /gene="yfbR"
                     /locus_tag="JQH51_000511"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY6074977.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21226..23058)
                     /locus_tag="JQH51_000512"
     CDS             complement(21226..23058)
                     /locus_tag="JQH51_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY6074978.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="JQH51_000513"
     CDS             complement(23145..23795)
                     /gene="hxpA"
                     /locus_tag="JQH51_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY6074979.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23806..24300)
                     /locus_tag="JQH51_000514"
     CDS             complement(23806..24300)
                     /locus_tag="JQH51_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY6074980.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24383..24838)
                     /locus_tag="JQH51_000515"
     CDS             complement(24383..24838)
                     /locus_tag="JQH51_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY6074981.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25176..26378
                     /gene="ackA"
                     /locus_tag="JQH51_000516"
     CDS             25176..26378
                     /gene="ackA"
                     /locus_tag="JQH51_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY6074982.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26453..28597
                     /gene="pta"
                     /locus_tag="JQH51_000517"
     CDS             26453..28597
                     /gene="pta"
                     /locus_tag="JQH51_000517"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY6074983.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28787..30307
                     /gene="yfcC"
                     /locus_tag="JQH51_000518"
     CDS             28787..30307
                     /gene="yfcC"
                     /locus_tag="JQH51_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY6074984.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="JQH51_000519"
     CDS             complement(30340..30882)
                     /gene="yfcD"
                     /locus_tag="JQH51_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY6074985.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="JQH51_000520"
     CDS             complement(30940..31491)
                     /gene="yfcE"
                     /locus_tag="JQH51_000520"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY6074986.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="JQH51_000521"
     CDS             complement(31547..32191)
                     /gene="yfcF"
                     /locus_tag="JQH51_000521"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY6074987.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32327..32974
                     /gene="yfcG"
                     /locus_tag="JQH51_000522"
     CDS             32327..32974
                     /gene="yfcG"
                     /locus_tag="JQH51_000522"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY6074988.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33031..33393
                     /gene="folX"
                     /locus_tag="JQH51_000523"
     CDS             33031..33393
                     /gene="folX"
                     /locus_tag="JQH51_000523"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY6074989.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33414..34307
                     /locus_tag="JQH51_000524"
     CDS             33414..34307
                     /locus_tag="JQH51_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY6074990.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34355..35245)
                     /locus_tag="JQH51_000525"
     CDS             complement(34355..35245)
                     /locus_tag="JQH51_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY6074991.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="JQH51_000526"
     CDS             complement(35442..36215)
                     /gene="hisP"
                     /locus_tag="JQH51_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY6074992.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36223..36939)
                     /locus_tag="JQH51_000527"
     CDS             complement(36223..36939)
                     /locus_tag="JQH51_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6074993.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36936..37622)
                     /locus_tag="JQH51_000528"
     CDS             complement(36936..37622)
                     /locus_tag="JQH51_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY6074994.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="JQH51_000529"
     CDS             complement(37712..38494)
                     /gene="hisJ"
                     /locus_tag="JQH51_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY6074995.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38715..39497)
                     /gene="argT"
                     /locus_tag="JQH51_000530"
     CDS             complement(38715..39497)
                     /gene="argT"
                     /locus_tag="JQH51_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY6074996.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39763..40332)
                     /locus_tag="JQH51_000531"
     CDS             complement(39763..40332)
                     /locus_tag="JQH51_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY6074997.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40427..41944)
                     /gene="purF"
                     /locus_tag="JQH51_000532"
     CDS             complement(40427..41944)
                     /gene="purF"
                     /locus_tag="JQH51_000532"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY6074998.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="JQH51_000533"
     CDS             complement(41981..42469)
                     /gene="cvpA"
                     /locus_tag="JQH51_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY6074999.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="JQH51_000534"
     CDS             complement(42728..43390)
                     /gene="dedD"
                     /locus_tag="JQH51_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY6075000.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43380..44648)
                     /gene="folC"
                     /locus_tag="JQH51_000535"
     CDS             complement(43380..44648)
                     /gene="folC"
                     /locus_tag="JQH51_000535"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY6075001.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44718..45632)
                     /gene="accD"
                     /locus_tag="JQH51_000536"
     CDS             complement(44718..45632)
                     /gene="accD"
                     /locus_tag="JQH51_000536"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY6075002.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45788..46447)
                     /locus_tag="JQH51_000537"
     CDS             complement(45788..46447)
                     /locus_tag="JQH51_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6075003.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46530..47342)
                     /gene="truA"
                     /locus_tag="JQH51_000538"
     CDS             complement(46530..47342)
                     /gene="truA"
                     /locus_tag="JQH51_000538"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY6075004.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47342..48355)
                     /locus_tag="JQH51_000539"
     CDS             complement(47342..48355)
                     /locus_tag="JQH51_000539"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY6075005.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="JQH51_000540"
     CDS             complement(48421..49557)
                     /gene="pdxB"
                     /locus_tag="JQH51_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY6075006.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49656..50651
                     /locus_tag="JQH51_000541"
     CDS             49656..50651
                     /locus_tag="JQH51_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY6075007.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50648..51809)
                     /locus_tag="JQH51_000542"
                     /pseudo
     CDS             complement(50648..51809)
                     /locus_tag="JQH51_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(52064..53305)
                     /gene="fabB"
                     /locus_tag="JQH51_000543"
     CDS             complement(52064..53305)
                     /gene="fabB"
                     /locus_tag="JQH51_000543"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY6075008.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53464..55470
                     /gene="mnmC"
                     /locus_tag="JQH51_000544"
     CDS             53464..55470
                     /gene="mnmC"
                     /locus_tag="JQH51_000544"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY6075009.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55591..55869)
                     /locus_tag="JQH51_000545"
     CDS             complement(55591..55869)
                     /locus_tag="JQH51_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY6075010.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55903..56451)
                     /locus_tag="JQH51_000546"
     CDS             complement(55903..56451)
                     /locus_tag="JQH51_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY6075011.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56451..57260)
                     /locus_tag="JQH51_000547"
     CDS             complement(56451..57260)
                     /locus_tag="JQH51_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY6075012.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57260..58084)
                     /gene="mepA"
                     /locus_tag="JQH51_000548"
     CDS             complement(57260..58084)
                     /gene="mepA"
                     /locus_tag="JQH51_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY6075013.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58088..59173)
                     /gene="aroC"
                     /locus_tag="JQH51_000549"
     CDS             complement(58088..59173)
                     /gene="aroC"
                     /locus_tag="JQH51_000549"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY6075014.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59208..60140)
                     /gene="prmB"
                     /locus_tag="JQH51_000550"
     CDS             complement(59208..60140)
                     /gene="prmB"
                     /locus_tag="JQH51_000550"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY6075015.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60306..60857
                     /gene="smrB"
                     /locus_tag="JQH51_000551"
     CDS             60306..60857
                     /gene="smrB"
                     /locus_tag="JQH51_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY6075016.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60961..61851)
                     /locus_tag="JQH51_000552"
     CDS             complement(60961..61851)
                     /locus_tag="JQH51_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY6075017.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61838..62362)
                     /locus_tag="JQH51_000553"
     CDS             complement(61838..62362)
                     /locus_tag="JQH51_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6075018.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62359..62829)
                     /locus_tag="JQH51_000554"
     CDS             complement(62359..62829)
                     /locus_tag="JQH51_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6075019.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62826..63374)
                     /locus_tag="JQH51_000555"
     CDS             complement(62826..63374)
                     /locus_tag="JQH51_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6075020.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63379..64101)
                     /locus_tag="JQH51_000556"
                     /pseudo
     CDS             complement(63379..64101)
                     /locus_tag="JQH51_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64121..66761)
                     /gene="yfcU"
                     /locus_tag="JQH51_000557"
                     /pseudo
     CDS             complement(64121..66761)
                     /gene="yfcU"
                     /locus_tag="JQH51_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66843..67406)
                     /locus_tag="JQH51_000558"
     CDS             complement(66843..67406)
                     /locus_tag="JQH51_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6075021.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16921 a        18274 c        16778 g        15681 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaatgac
      601 gccttttatt gataatttac atatgaaaaa attttccttt tgttatatta tttctgtttt
      661 cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa cgccccgaca
      721 cgtaagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg tgcatccacg
      781 taatgccggc aatattgata atctgaatag gtccgcagag tatataaaag agttctttgt
      841 cagtagcggt gccagagtta ccgcgcaggg cgtccccgat tggctattac gactctgcgc
      901 ctggaagcca gaattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact
      961 ttattgctgt ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcggcat
     1021 tgttgtcatc ccgggattta tctgtttatt ctatgaatgc cccagggttt attcccggta
     1081 ttgatttttc tgatcacctc aattattggc aacacgatat tcccgccgta atgattactg
     1141 acaccgcttt ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga
     1201 attatcagaa aatggctcag gtggtagatg gtgttataac cttgttatac aacagtaaat
     1261 aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat tttccctcgt
     1321 ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga atgattggct
     1381 attttgcaga aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt
     1441 tgatggataa tatacatgac acgctttcag gattacgccg actggaatca ctggatcgaa
     1501 gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag
     1561 tgaccttttc agaagagtta gaggggcaac tctttgcctc gatcatgctt gatatggaaa
     1621 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     1681 ttgggtgatg gtgtcagagc gccaaaaaaa accgccgaac atgtcggcgg ctttctgact
     1741 tacaaactaa cagtttacat cagcggcatt gctaaccgca caatactaat cagcggttgt
     1801 ggccatacac ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg
     1861 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg
     1921 ctcaccgcca cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc
     1981 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag
     2041 cccagcgtca tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg
     2101 atcggacgat gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga
     2161 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg
     2221 gccaggtaaa cccctaccgc ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc
     2281 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc
     2341 tggctcaacg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact
     2401 acgcgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg
     2461 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2521 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2581 atcatcatgc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt
     2641 ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccgaac
     2701 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa
     2761 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata
     2821 ccgaggaaca gagacgccag atggtttgca ttcgccagca ggatcccgcc cagcgcggca
     2881 attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag
     2941 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3001 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac aaaccagagc
     3061 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3121 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3181 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3241 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3301 tgcggataga agcccagcag taccagcagc accaccagca acaggatcat aaacagctca
     3361 cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa
     3421 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3481 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3541 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3601 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     3661 taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     3721 attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag cacgaagccc
     3781 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc
     3841 cacgcaccgt agaagatgcc gataacaccc agccacatag cgattggcgc gaactctgcc
     3901 gacgcgttcg ggaacagcgg cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc
     3961 aagatccccg cgaggtccac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4021 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatc
     4081 aacagatatt ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4141 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcgat caacatcacc
     4201 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4261 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4321 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4381 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4441 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagaccgtca
     4501 atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4561 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4621 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     4681 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     4741 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     4801 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc
     4861 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     4921 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     4981 cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa gaaccgccgg
     5041 gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5101 cttgacgaac actttgtcgt acagccagtc aaagccccaa gcgttgtacc accaggtact
     5161 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagaacgtt tacccagcca
     5221 cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt
     5281 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5341 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5401 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5461 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5521 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5581 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     5641 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     5701 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     5761 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     5821 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     5881 cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca tcaggaacag
     5941 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag
     6001 cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg
     6061 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6121 gccgtcagca aagtgcgctg gtgccagttc caccatatcg cggaagttca gggtgcccag
     6181 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6241 gaacgctttc attgctgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6301 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag
     6361 aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6421 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6481 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6541 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6601 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     6661 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     6721 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     6781 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     6841 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     6901 cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     6961 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc
     7021 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7081 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7141 ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7201 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7261 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccgggc
     7321 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7381 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7441 ccagcgcacc ggcgaagtaa gcgcctagtg agaagaacac cccggaaatc gccagcagcg
     7501 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7561 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7621 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     7681 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     7741 gatcagcaga tcctctttct cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     7801 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     7861 gcagcgtgag aagttaatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     7921 tttttgcagc gagatacagc cgaccgggca ggctaccgca cagaggttac aggctacgca
     7981 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8041 atagaccggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8101 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8161 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8221 agcgtcagcg gcaggcagat tttccagccg aaggacatta cccggtcata acgcggacgc
     8281 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8341 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8401 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8461 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8521 tgacggtgac acaccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8581 atgacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     8641 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     8701 gaagcacgca tcgcacccag cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc
     8761 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     8821 gcaaccaccc aacccggact gactggcaca attgcaaagg ccagcagcag cgaggtaaag
     8881 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     8941 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9001 ttaggtccat aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9061 gccccacagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9121 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9181 cgccagccag caccggagca atgccggaca tacccatcgg caagcccacc tgccctgccg
     9241 tcagtccttc ggcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga
     9301 cgcgtgtacc tgcgttcaca cccaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9361 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9421 ggtggtagta cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9481 tggtgaagta atccagaccg ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9541 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9601 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcggt cggctggttg ttaccttcca
     9661 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
     9721 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
     9781 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc cgccagttct gggattttcg
     9841 ccacaacagc gtcaattaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg
     9901 tgctgtgcag cgagtgcagc cagcgccagc tttccagcat gacagttttg ctgtcgtaat
     9961 aagccggatc gtaaacctgg aagaaacgtt gggcgcgacc ttcattatta attaccgtac
    10021 cgtcgctttc agcaaagctg gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg
    10081 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10141 tagcagaagc gtaacgatgc agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg
    10201 tttccagttc ggttaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10261 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10321 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10381 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ttttgcagct
    10441 caggttcgat accgtcaacc gctggtgcgg agttatccag cgcatgggcg atggcaaaac
    10501 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10561 gagtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10621 ttgccgcaat ctgccagtca gccactttct gtgctgcagc catttcgcgc gctttacctt
    10681 tcaccgcctg acgcactgcc agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    10741 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    10801 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    10861 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    10921 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    10981 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11041 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11101 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11161 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11221 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11281 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11341 tgtcgtgcgc gccataaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11401 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11461 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg
    11521 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11581 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    11641 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    11701 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    11761 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    11821 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    11881 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    11941 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12001 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12061 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12121 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12181 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12241 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12301 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12361 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12421 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12481 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12541 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12601 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    12661 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    12721 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    12781 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    12841 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    12901 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    12961 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13021 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc
    13081 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13141 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13201 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13261 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13321 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13381 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13441 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13501 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13561 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13621 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    13681 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    13741 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    13801 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    13861 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    13921 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    13981 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14041 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14101 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14161 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14221 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14281 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14341 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14401 cggcatccag tcgaggaact cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc
    14461 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14521 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14581 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    14641 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    14701 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    14761 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    14821 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    14881 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc
    14941 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15001 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag
    15061 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15121 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15181 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15241 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15301 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15361 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15421 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15481 ttcgccaatt accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt
    15541 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15601 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    15661 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    15721 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    15781 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    15841 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    15901 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    15961 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16021 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16081 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16141 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16201 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16261 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16321 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16381 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16441 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16501 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16561 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16621 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    16681 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    16741 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    16801 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    16861 aaaaacctta caataactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    16921 cctttctata accttttgtc aactttaaca aaagcttctt cacattagtt tacataatat
    16981 caacaccatt agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta
    17041 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17101 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17161 gttattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag
    17221 gtgatttttt tcaggcgatt atttgtgcat tcgggacgtg aatctctggt gattgaaaaa
    17281 taaacagttt tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct
    17341 ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga attatctatc
    17401 gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga
    17461 cattgggctg tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc
    17521 cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg
    17581 taagccatct actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc
    17641 cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata
    17701 agccagtcgc catggaatat ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg
    17761 acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc
    17821 tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg
    17881 atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt
    17941 acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag
    18001 gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag
    18061 attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta
    18121 accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag
    18181 ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg
    18241 acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct
    18301 cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt
    18361 actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg
    18421 taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt
    18481 tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat
    18541 atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt
    18601 gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac
    18661 gcgaacacgt acaaacagca atggtgaagc ggcatcaaga gcagggatcc gtcatttatg
    18721 cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa
    18781 agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt
    18841 gaatattaaa taataattaa ttagcatcat cctcattcat taattccgct taacaatagt
    18901 ttcacacctt acccccattg cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa
    18961 tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat tttcacacaa
    19021 ttatcttaca gataaaaaaa ccagacttgc aattaagaat tagaacaatt cactatataa
    19081 cattgcatgt aaagcatata agcctcatta tttgtcatta ttaagttatt aacagcacaa
    19141 tcgagccttc ccctctggca aaatcttatt ctgcagacct tcaaaacacc gtcctggggg
    19201 agtacattgt tctaagctga cttccacggc agggagtggc gataacagca aaaaaggtca
    19261 agattcatgt cccccattga aaaatccagc aaattagaga atgtctgtta tgacatccgt
    19321 ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag gtaacaaggt actgaaactg
    19381 aacatcggca acccagcccc attcggtttt gacgcgccag atgaaatcct cgttgacgtg
    19441 atacgcaacc tgcctaccgc tcaagggtat tgcgattcca aagggcttta ctccgcgcgt
    19501 aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt
    19561 tacatcggca atggtgtatc ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc
    19621 ggggacgaaa tgttggttcc tgcaccagat tacccactct ggaccgcggc ggtttcgctt
    19681 tccagcggta aagcggtgca ttatctttgc gatgaatcct ctgactggtt cccggacctc
    19741 gatgatattc gcgctaaaat tacgcctcgt acgcgtggga ttgttattat caacccaaat
    19801 aacccaaccg gcgcggtata ttccaaagag cttttaatgg agattgtgga gattgcacgt
    19861 cagcataatc tcattatctt cgccgatgaa atttatgaca aaattctcta cgacgatgct
    19921 gagcatcact caattgcgcc gctggcacct gacctgctga ccattacctt taacggactg
    19981 tcgaaaacgt accgcgttgc aggcttccgt caggggtgga tggtgttgaa cgggccgaaa
    20041 aaacacgcca aaggctacat cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct
    20101 aacgttcctg cgcaacacgc cattcagacc gcgctgggtg gttatcagag catcagtgaa
    20161 tttattaccc ctggcggtcg tctttatgag cagcgtaacc gcgcgtggga actgatcaac
    20221 gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc
    20281 gacgccaaac gctttaacat tcatgacgat cagaaaatgg tgctggattt cctgttgcag
    20341 gaaaaagttc tgttggtgca agggacggca ttcaactggc cgtggccgga tcacttccgc
    20401 attgtcacgc taccgcgtgt cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc
    20461 ctttctggtt atcatcagct gtaatcttaa tttcactgcc ggagattgca tccggcagtg
    20521 ttatcccgcc acaatgacct gatgatgtca tcatacgtaa ggtcactatg aaacagagcc
    20581 atttttttgc ccatctctcc cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg
    20641 tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg
    20701 cagctatcaa aaatcggaaa tttggcggta atgtcaacgc cgaacgtatc gctttactgg
    20761 cgatgtacca cgatgcctca gaagtgctca ccggcgatct ccctactccg gtgaaatact
    20821 tcaatgcgca aatcgctcag gagtacaagg ctattgaaaa aatcgctcag cagaaactgg
    20881 tcgatatggt tccggaagaa ctgcgggata tctttgcgcc gttaattgac gagcatgcat
    20941 atagcgatga agaaaaatcg ctggtgaaac aggcagatgc actgtgtgca tatctgaaat
    21001 gtctggaaga actcgcggcc ggaaataatg aattcttgct ggcaaaaacg cgactggaag
    21061 cgacgcttga agcgcgtcgc agccaggaga tggactactt catggaagta tttgttccca
    21121 gcttccatct ttcgctcgat gagattagcc aggattcacc gctgtaagca gccggagtct
    21181 gcgtcgcatc agtcaataag cgccggatgc gacatcaggc tcttgtcaaa acggaaacag
    21241 catcgggatc atcaccacac aaaccgccat cacgataatg gtgaacggta cccccaactt
    21301 cacaaagtca ctgaagctgt aatttcccgg acctaaaacc agggtgttaa caggtgaaga
    21361 caccggtgtc ataaaggcgg cggatgctgc catcgccacg accatcgcaa atggataagg
    21421 cgacactccc atcgttttgg cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc
    21481 ggtattagag ataaacagcc caataaccgc cgacaagaca aacaaacacc ccagcatcat
    21541 atgtggccca taaccgccgc caatatccat cagccctttc accgccagcg cgacacctcc
    21601 cgttttctgt aatgccacag caaacggcat catcccaacg atcaaaataa tgctcggcca
    21661 gtgaatggat ttataggcgc tttcagcatc tatacagcgg aatttcccca tcagcaggca
    21721 ggcgatgata gcggcaacag gattaggaat ttcatttgtc agcattaacg ccaccattag
    21781 caccagacag aaaatggcat ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc
    21841 ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg gccagcatac cgatcagttt
    21901 ccagttaccc acaaccagga tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc
    21961 ttccagcgcc acgccattgc gcttgagccc caccacattc agtccgtagc gggtacgaaa
    22021 accaatttcg cgcaccgatt taccaatcag ttctgactca ggaattaatg aaatctctgc
    22081 catgcccaca tcaagggcct ggtcagaaaa atactcgccg cgcagtacca tcggctccag
    22141 caattgctca ctacaaaatt gccggagatc gacatcagcc gcagacatat caataagcaa
    22201 aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc acgataacgc gacgaaaacg
    22261 ccgccagcgt tcaacaccga tgacgttagc gccataacgc tcacgtaatt tgagatcatc
    22321 cagccgttga cctatcattg gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt
    22381 cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg
    22441 ggtctgggta tcccctttca gcatgaaacg catcactaac atatacaaaa tacccagcac
    22501 cagcacgacc aggccaatag gtgttacgct aaagaaactg aagccgtgat agccttcacg
    22561 cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct
    22621 aatcagcccg gcaaaactta acggcatcat cagacgtgac ggcgaagttt gcatacgcat
    22681 ggcaacgctt aacaccacgg gaataaagat agcgacaacg ctggttgaac tcataaacgc
    22741 gccaagcccc gcgacggtca gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc
    22801 cactttgacc agccatgttc ccattacggt ggcaacaccg gtacggacca aaccatcgcc
    22861 aataataaac aaggcggcaa tcaggacaac gttaggatca gaaaagccgg aaaatacttc
    22921 ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc
    22981 catacgcact ctgcccgtcg caaacaagac gatggcaacg gccagtaatg aaagaaccca
    23041 aatcaattca ccgttcacaa cttatccttg ttaattgagg gggatgactt gattctgcca
    23101 taaaaaagcc ccgacgagac ggggctaaat catgatcagg tgtttcactg aataataaca
    23161 tcgccatttg gctgtttggt cacagtaatt tgctccagac tgtggaggac caaatcgacc
    23221 tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa tgacatggca acccgccgcc
    23281 aggccagaaa gcacgccagc gggagcatct tccaccacca cacactcctg cggcgcaagc
    23341 cccagcagct gcgcacctaa cagatacgca tcaggttctg gttttccgcg cttcactcgc
    23401 tcagcggtta caaacacctc tggtgcggga agccccgcta ttttatggcg cgctcgcgct
    23461 accggcatgg agccagaagt cacaatggcc cacggaatac ctgctttatt caaatgattg
    23521 agtaaggcga ttgccccagg aagcgcggta ataccttcgg tttccgtggc ctcgatttgc
    23581 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
    23641 aaagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
    23701 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
    23761 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cgttgataat
    23821 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
    23881 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccatgag tcccagcgtc
    23941 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
    24001 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
    24061 ctgcaaatta gaccaagaaa catacaacgc atgatacata tccataatgt cgatgatagt
    24121 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
    24181 gtaaccacgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
    24241 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
    24301 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
    24361 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
    24421 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
    24481 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
    24541 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
    24601 aggcgaacag tgcagtggca actgccggcc caagcagacc gcccagggca atctgccagc
    24661 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
    24721 cgcgattttc gacaaagacc ggagcaaggc gtttttccag tggccacgtc tttgagtaat
    24781 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
    24841 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
    24901 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
    24961 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
    25021 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
    25081 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
    25141 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
    25201 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
    25261 acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg
    25321 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
    25381 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
    25441 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
    25501 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
    25561 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
    25621 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctatgcc ctgccgtaca
    25681 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
    25741 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
    25801 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
    25861 ctatgggtct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
    25921 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
    25981 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
    26041 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
    26101 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
    26161 cattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
    26221 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
    26281 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
    26341 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
    26401 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
    26461 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
    26521 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
    26581 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
    26641 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
    26701 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
    26761 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
    26821 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
    26881 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gtggtgccaa
    26941 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
    27001 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
    27061 tgttgatccg gcgaagctgc aagaatctag cccgctgccg gttctcggcg ctgtgccgtg
    27121 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
    27181 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
    27241 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
    27301 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
    27361 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
    27421 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
    27481 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
    27541 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
    27601 cagccgtcgt ctgtctccgc cagcgttccg ttatcagctg actgaactgg cgcgcaaagc
    27661 gggcaaacgt atcgttctgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
    27721 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
    27781 tgttgcagcg tctcagggtg tagaactagg tgcaggcatt gaaatcgttg atccagaagt
    27841 ggttcgcgaa agctatgttg gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga
    27901 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
    27961 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
    28021 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
    28081 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
    28141 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
    28201 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
    28261 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
    28321 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
    28381 tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg gtaacaccac
    28441 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
    28501 gcgcaaaccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
    28561 cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc cacagctttg
    28621 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatttg
    28681 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatctca
    28741 ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
    28801 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    28861 tgctatttta accagtcttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    28921 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    28981 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    29041 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa gggttaacct caggatcgaa
    29101 atacgggaca gccgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    29161 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    29221 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    29281 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    29341 gggctatgac agtattacca ccgtcctggt gacttatatt gccacgcaaa tcggttttgc
    29401 cagttcgtgg atgaacccgt tttgtgtggt tgttgctcag gggattgccg gcgttccggt
    29461 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga ttggcctgat
    29521 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    29581 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    29641 tgactggctg gtattgattg tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat
    29701 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    29761 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    29821 ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    29881 gattttgctg ctggttggta atggtgaagc aggtaatgcc agcgtgttaa atactatcct
    29941 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    30001 gctcttccag gctgtattta atttcttcgt gacgtccggt tctggtcagg cggcattaac
    30061 tatgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    30121 ggcgttccag tttggtgatg gcttcagcca catcatttac ccaacctcag cttcgttaat
    30181 ggcaacgctc ggtgtgtgcc gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    30241 gcttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    30301 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    30361 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    30421 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa ctccactgac
    30481 ttcatcttcc tgtagggcga agggaccgtg agagacgcaa ctgaacaacg cgccccagac
    30541 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    30601 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    30661 tacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    30721 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    30781 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    30841 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    30901 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    30961 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    31021 ataacccgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    31081 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    31141 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    31201 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca tcatttgatc
    31261 cacttcgctg tcgcagttgc cacgcacagc gataacctta tgtgccactt cattaagccg
    31321 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    31381 cacgtcgcca aggatcacca gccactgggc accgctttgg acaaacaact ccacaacacg
    31441 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    31501 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    31561 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    31621 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    31681 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    31741 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    31801 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    31861 tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    31921 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    31981 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    32041 gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    32101 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    32161 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    32221 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    32281 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    32341 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    32401 ttatcgcttg attaaggtag acttggggaa aggcggtcag tttcgtccgg aatttttgcg
    32461 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    32521 accgttaagc ctctttgagg ctgctgccat tttgttgtat ctggctgaga aaacaggact
    32581 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    32641 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    32701 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    32761 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    32821 ctgctggccg tgggttaatg cctggactcg cctgcgaatt gacctcgcaa tgtatccggc
    32881 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    32941 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    33001 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    33061 aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    33121 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    33181 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    33241 aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    33301 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    33361 tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc tgcatgaaca
    33421 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    33481 tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc gttctcggcc
    33541 ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg
    33601 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacaag caaaaagagc
    33661 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgctagcg
    33721 acacgccgcc gtcggtactc atttccggct cggcaacggg ctattatggc gacttaggtg
    33781 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    33841 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    33901 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    33961 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    34021 atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca tttaatatgg
    34081 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc
    34141 gcccggctat tttgcgcgtc cccacaaccg ccattcggct gttaatgggc gaatcttcag
    34201 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    34261 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    34321 aaacatccgc cagttaactc cctgtgttac aggattagtg gctttgcgcg ataagatcgt
    34381 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    34441 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    34501 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    34561 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    34621 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    34681 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    34741 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    34801 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    34861 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    34921 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    34981 cttccggctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    35041 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    35101 gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    35161 taataaaatt ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg
    35221 gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg
    35281 acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga cagcgaattc
    35341 agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact cgcaaattcc
    35401 ccttgaaaat actccggcag cgttagccac cggagaggaa attatttcag cgatccctta
    35461 aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg cgcgccctct
    35521 tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg agcaaagccc
    35581 atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg cataatacgt
    35641 aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa cagtaaaact
    35701 tctggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc gccggaaaga
    35761 tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag atacttcacc
    35821 gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc ttccatgacg
    35881 ttttccaaca ccgtcatatg gctccagaga ttgaagtgct ggaataccat cgtcaggcgt
    35941 gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc tttgtcgcgc
    36001 accagattga tcgtctggcc attgaccacg atcgatcctt cactcggttt ttcgaggaag
    36061 ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat gcttattaca
    36121 tctccggcat tcgcttgcag tgagacccct ttcagcactt cgtgttcgcc atagcgtttg
    36181 tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg ttgaagaagg
    36241 tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca ggacataaga
    36301 gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat acgtggcggc
    36361 gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt
    36421 agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac gcagcgcaga
    36481 aggcaaaata atgcagcgat acattttaaa ggtcgagaag ccataggctc tggcggcttc
    36541 aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag tgtaagcgca
    36601 ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga aagcgttaag
    36661 gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca ccagcaactg
    36721 aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa actggatgta
    36781 tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc ctatcactac
    36841 cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac cgtcggtcca
    36901 cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct cttcacaccc
    36961 acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacggtggt gaaaaccagg
    37021 taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt gcctgccagc
    37081 tgcgtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt agatttgagg
    37141 atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc cggaaacatg
    37201 atccgccgaa acacttgccc gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct
    37261 ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc gtagataaaa
    37321 ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc
    37381 tccgtcacca tgtttagcgc aatctgcaaa ccatagaaaa tcagcagcat caacaccaag
    37441 tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa tcagccccga aagccgattt
    37501 tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac tacagagctg
    37561 atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac
    37621 atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc cggttacccg
    37681 aagggagcgg gctttctttc accgatggcg attaaccacc ataaacatca aaatcgaagt
    37741 actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag gctttgttca
    37801 gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct acgccaaaca
    37861 gtttttcatc tttaacagac ggaccaccga atttgtaatc tttaccgacg ggttgtttga
    37921 ggaaaccttc gctggcagcg acctcatcct ggaacgcggc atcaatacgt ccggcagtca
    37981 ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct tttggtgccc
    38041 agtgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt
    38101 tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaga cgagaatcgg
    38161 cagcgtacag tttgtcggtg aaggcaattt cttgctgacg tttttccgta atggaaagcg
    38221 atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca tccagcgggt
    38281 tttcgacaaa cgtacattgc gtattgatgc gcttgcacaa ctccttcgcc agctcgatat
    38341 cgaaaccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg
    38401 taccgatgcg gatgttttgc ggaattgcag caagtgccgc agttgcgctg gagaaggcca
    38461 gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac agtcttttta
    38521 tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca tcaaggcaaa
    38581 atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat gcatttcaat
    38641 gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat caggtacagc
    38701 ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt cgccatcttg
    38761 tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc cgtcagttca
    38821 gcataatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt gtcttttact
    38881 gatgagccag caaaagcgaa atctttacca gcaggttgct tgaggaatcc ttcgctggca
    38941 gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga atagaccaaa
    39001 tcctggttgg cataggccac cacatccacg cctttactac gccaggtctc gttagcgtaa
    39061 gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga atccagcgtt
    39121 ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg
    39181 gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg
    39241 atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac ccaggtacat
    39301 ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc aacaaaatca
    39361 cctttagcat ctttcgacga gaacagcgca taggtggtat cggttccgat acgtaccgtc
    39421 tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaataa agacagagcg
    39481 agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg
    39541 tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga caacagtaac
    39601 attcaacgtt aaatatgtta ataagacatt gcattattgt cctgaagttg aagatagcag
    39661 gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg
    39721 atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg
    39781 ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat taaccgtctg
    39841 atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg gaggcataat
    39901 caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt gcaatggtgt
    39961 ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg ccgcacgggt
    40021 cagtaagcca tcagtatagc tatggacaat gccggacagg gttttgattg aacagggtaa
    40081 aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc
    40141 atcgagcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga
    40201 taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg
    40261 cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga tgcctacaat
    40321 gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca gtatttcggc
    40381 gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat ccttcgttat
    40441 gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca tcattacgta
    40501 acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag acgccgttga
    40561 acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg tcgatcagat
    40621 cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt tcatcaactt
    40681 cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa acgttcggga
    40741 agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct tcgcgtgcca
    40801 tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt
    40861 ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac ttacgacgca
    40921 gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg aagccctggc
    40981 ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag gtttctggga
    41041 tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc tcgcccagtt
    41101 tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc
    41161 gggcaaagta tacatactca aacaggcacg gattgctgac cggattgtca gcacattgac
    41221 gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca
    41281 ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata tattctgtac
    41341 ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt ggatcgcgga
    41401 aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg cggattaagc
    41461 ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt
    41521 tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg gtgttgatgt
    41581 ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc agattgccgt
    41641 tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct tcagaggcgc
    41701 tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc tgcaaacgct
    41761 gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc aaacggaagc
    41821 agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc tgaagcaccg
    41881 ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg acaataccgc
    41941 acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg ggcaagaaac
    42001 ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat tgcgggatca
    42061 gctgtgattt gctccagtct tcacttttcg acaccccggt aaaggagtcg agaaagaaga
    42121 gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc agcacccgat
    42181 cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc acgatagcac
    42241 caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga accagttcgt
    42301 cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca acaaagaaag
    42361 cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgtggatc aggctaacca
    42421 gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata tgtgtcccac
    42481 gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa aacgtttgcg
    42541 tagggatttc cttcccgcgc atcaataaaa atggcgctga aaaaatattc aacgccatcg
    42601 actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc
    42661 cgacctacgg ttctaacact gcccaagcct gataagacgc gccagcgtcg catcaggcaa
    42721 gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa agttgcttca
    42781 actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc agaatacggg
    42841 taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca
    42901 gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt tgcacaacat
    42961 aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg
    43021 caggtggtac ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc ggtggggcga
    43081 ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc ggatcgagcg
    43141 acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg
    43201 gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc gctttcggca
    43261 ccagcgggat agccgcgaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg
    43321 gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact
    43381 tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa
    43441 gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc
    43501 tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg
    43561 gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag
    43621 gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg
    43681 tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg
    43741 acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg
    43801 ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca gcccgctggc acgcagtgcc
    43861 gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcagatt ttccagcgtg
    43921 ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc
    43981 cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc
    44041 ggctcaccga caattgccgg tttttcgctg cggaagatgc ctgctttctc gagaccaata
    44101 ctttcgcgat ctggacccag ccagtcggta tgatccagcg caatactggt tactaccgcg
    44161 acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc
    44221 accacgtcaa gttgtgcctg cttgaacagc cacaacgccg acagcgtacc gtactcgaaa
    44281 taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc
    44341 gattccggca attcctggcc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc
    44401 gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag
    44461 gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcaggacg
    44521 ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc
    44581 aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg
    44641 ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc
    44701 cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg
    44761 ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc
    44821 gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc
    44881 aggaattcac tgcgctggaa tctaggcggc agtttttcgc gaacggtctg ttcgataaca
    44941 cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc gcccagcatg
    45001 gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag
    45061 ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc
    45121 agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct
    45181 tcctgcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc cattgaaccg
    45241 cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc
    45301 atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag acggtcttta
    45361 tacttcttgg agtcacgaaa cttcagctca tctttcggct caagctcgct acccagctcc
    45421 acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga
    45481 tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga
    45541 ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta
    45601 atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc
    45661 gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat
    45721 gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag
    45781 ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc
    45841 ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca
    45901 tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc
    45961 cgatcacgtt ataagcagcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg
    46021 gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt
    46081 tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc gaattgggat
    46141 tactgaataa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa
    46201 tcgcggcgat cagcatcagc actaccatca tatggacgtt gagatcgttg gtttccagtg
    46261 atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc attaccacca
    46321 gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg
    46381 cgactagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca
    46441 ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac
    46501 agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc
    46561 ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct
    46621 tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc
    46681 tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga
    46741 tgtacaaagg cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta
    46801 atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg
    46861 aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt
    46921 tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg
    46981 atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct
    47041 ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc
    47101 gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct
    47161 gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc
    47221 ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc gtaatactta
    47281 ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg ctggtcggac
    47341 attagtacag atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc
    47401 caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat
    47461 tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc tgagtcggga
    47521 attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca cgcgcttctt
    47581 ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg tagaataccg
    47641 gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt
    47701 cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac aacggcagca
    47761 tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca
    47821 atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa
    47881 tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt ttcagtgccg
    47941 ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg cggtaatcgg
    48001 tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca
    48061 gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg
    48121 tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg
    48181 catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac
    48241 gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg
    48301 cttcgcccat agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg
    48361 tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga
    48421 ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact
    48481 ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg
    48541 cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg
    48601 cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagca gaccatgcag
    48661 cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg
    48721 cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag
    48781 ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag
    48841 ttccggctcg ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca
    48901 ggtcagcagc gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc
    48961 gggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt acggaccatc
    49021 tttaaagagt ggcgtatgga aagtcagaat atccgcgcgc tggactaact catccagcga
    49081 gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat
    49141 ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc
    49201 caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa
    49261 aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc
    49321 ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagttc caacaaattt
    49381 aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc
    49441 atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc
    49501 caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt
    49561 tgtgttacct gtatgagacg agagttaacc ggacaagtat gccataatct cgcggccagg
    49621 catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc
    49681 ctcctgggca gccaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt
    49741 tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc
    49801 aacaacaaag agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt
    49861 caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg
    49921 ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc
    49981 tggggaatca tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc
    50041 tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga
    50101 tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta ccggcggagc
    50161 acaatgcgct aaaacagctg gtgaccaaac ttacggcggc aacgggggaa cccagcaaac
    50221 agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga
    50281 aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc cagcaaaata
    50341 cgccgacgct ggaatcacta cagatggcgc taaaacaacc tttagatgcc agtgaactgg
    50401 cggcgttatc ggcatatatc cagcaaaaat atggcctttc tgcgcaatca tcgctttctt
    50461 ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa gggattgatc
    50521 cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata
    50581 tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc
    50641 tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc accgcagaga
    50701 tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg
    50761 gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc
    50821 gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac
    50881 atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg gcttgccaga
    50941 gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc acgccgccaa
    51001 aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg
    51061 taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc
    51121 cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc
    51181 cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc
    51241 cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac
    51301 cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg
    51361 ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc
    51421 aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa cgcccgatga
    51481 ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc cagaccacaa
    51541 gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggcg
    51601 taaccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatgcc gaccatggta
    51661 ttgccatagc ccagatcatg atgaacaaac agcgggataa ccggcaacgg caaccctacg
    51721 gtcatgtagg tgagaaaaac cgcaaaagag atgcggaaga gcgaaaaact ggcagaagat
    51781 cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg
    51841 agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgcg
    51901 tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctagaactg gtggcgattg
    51961 tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg gaggccgcat
    52021 tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc ggcctactgc
    52081 gaattaatct tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc
    52141 tgttagacat aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg
    52201 cagcctgctc gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca
    52261 tcagcagaga gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg
    52321 ctttggttgc agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct
    52381 ctttcacgtc gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggggtat
    52441 caacgccatg catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca
    52501 tgtctgcacc atcagaggtt gcgccgtagc caacgatttc agcatagatg taagcaccac
    52561 gcgccagcgc gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga
    52621 aaccgtcacg gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag
    52681 tagatagcgc acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc
    52741 cgccagcaaa cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac
    52801 agtgtgcgga agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg
    52861 gcgtggcgag acaggcagaa acgccggatg ccatcgcttt agtgaccaca tacgggccaa
    52921 ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg
    52981 aaccgccgcc ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag
    53041 agaggcccgc atcagcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc
    53101 tcataaagcg cacaactttg cggtcaatga ggccagtggt atccagtttt atgttgcccc
    53161 agacgtggct acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc
    53221 cttcacgcag agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca
    53281 ggccagtaat cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt
    53341 cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga
    53401 tatttgcgca gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact
    53461 tacgtgaaac actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct
    53521 gtttcccgag attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt
    53581 tatgtttttc tgggaggtaa ccaattagag gtacgctttc ctgagcatcc acatcctctg
    53641 tttgtgatag cagagagcgg cttcggcacc ggattaaact tcctgacgct atggaaggca
    53701 tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt
    53761 tttgagaaat ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa
    53821 ctggctccgt gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat
    53881 cgtttattgc tcgatgaaag ccgcgtgacg ctggatttat ggtttggcga tattaacgaa
    53941 ctgaccagcc aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc
    54001 tttgcgccag ctaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg
    54061 ttggcgcgtc caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt
    54121 ttgcaggaag ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg
    54181 ctttgtgggg tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt
    54241 acgggcagca gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg
    54301 tcgctggcgt tattacggcg cggctggcag gtagcgcttt attgcgcgga tgaagccccc
    54361 gcgctgggtg cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat
    54421 gaggcgctaa accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa
    54481 ttacccgtta aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa
    54541 agccagcata aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc
    54601 gttgaggcaa atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact
    54661 tatccgcaag gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg
    54721 caacagcagg gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat
    54781 gactgttggt tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg
    54841 gcgaacgggc atcaaatcag ccgtttcagc caaacgtcga ctctcccggt gtattcggtt
    54901 gccgggcagg tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg
    54961 tgctatgacg gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc
    55021 agttatcatc gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc
    55081 cagcggttga ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat
    55141 aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat
    55201 gttcccgatt atgaggcaac actcgtggaa tatgcgtcgt tggcggagca aaaagatgag
    55261 gcggtaagcg cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt
    55321 ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt
    55381 ccgatggatg ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg
    55441 ttgaagggta aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc
    55501 gtgaacgcct tatccaacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg
    55561 catcaggcat ggtgctccag acgccgcaac ttacagctgt gacgcctgct gaaacaggtt
    55621 ttcccacata tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc
    55681 tttttccaga ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact
    55741 ttccagttct gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc
    55801 atcatcactg gcatggtcaa ccatgccgtc gattaatgcc agaatgcgtg attcaaactc
    55861 cgcgatcatc ttctttcctc attgtaaaac gtggcgctgg cttcagttga gcgcttccgg
    55921 ccacgggaac tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat
    55981 gaagcgtgcc gggcgttccg gaatgccatt tgtcagataa tccatcacct gcgcatgtac
    56041 ccgccgttga aacaccacac gatccggttc gaaatcccct tccagattgt cgcaactgac
    56101 gttaaacgga tatccagccg ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc
    56161 aacatcttca aactggcttt gcgtctgggc atcgcgtccg tccgggcaat accagtagcc
    56221 gaaatcaacc agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat
    56281 tgcgctggca taaaagccat gagcaaagac gattcggtta tacggtactt ccgcatcagc
    56341 aggaagatag atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa
    56401 gcagctatta aaaatttcaa tcaactgctc gtagtggtgt gtactgttca ttagttcatc
    56461 cccaaccagt ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc
    56521 gccgagacga taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccaaccgt
    56581 gaacccatgc gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc
    56641 actttgccgc cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat
    56701 ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg
    56761 taaaacgatc cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca
    56821 attaacgcga agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc
    56881 atcagcaaga aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca
    56941 tcagcctgaa cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca
    57001 atattgagtt tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg
    57061 gaaatagagc cgccgcaggc ttgcagttta ttggttgcca gcgcgttagc gggagacatt
    57121 cccgcagcca tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata
    57181 aatcccgcca gcatggcgac aaaaaagaga actcccagca acagcggaga aaccataaac
    57241 aggctattaa acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg
    57301 gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc
    57361 agctttgcag ttctgccccg caaccatcgc ctgatggcgg taaaggttga tcttcacact
    57421 ccagactatc ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg
    57481 gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa
    57541 tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc
    57601 tgaaaatttc tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt
    57661 ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa
    57721 agatatcgac atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag
    57781 cgggcatccc catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac
    57841 tcagacgctg gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac
    57901 gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat
    57961 tagaaaaact gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg
    58021 gcgtcgctgc caggctagca ctactggcaa gcagagccag cagcgcaatc gcggttttgt
    58081 tcatttttta ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta
    58141 acaggtgatc cattaaaacg atcgccagca tcgcttcagc gatcggcact gcacggatcc
    58201 cgacacaggg atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa
    58261 tagtgcgccc cggcacggta atgctggagg tcggtttcag cgccatatga gcaatgattt
    58321 gctgcccgct gctgataccg ccgaaaatgc cgcccgcatg gttgctctgg aaaccgtctt
    58381 tggtgatttc gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa
    58441 tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc
    58501 ggtcaaagac cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg
    58561 cgccgatgga gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt
    58621 tgtcggggtc cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca
    58681 gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga
    58741 gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac
    58801 gtccaccgcc gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc
    58861 ctggacggaa gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga
    58921 tcaacaagcc aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga
    58981 cctgatccgg ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa
    59041 ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc
    59101 agccgagcgc cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg
    59161 tgtttccagc catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa
    59221 atcgcgaaat gttctcgtgc ggcaataagc tgctctttag tgagcataaa cacaccatcg
    59281 ccgccgttat caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt
    59341 accatgctgt tgcccacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg
    59401 ttaccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc
    59461 ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg
    59521 ttagtgacaa tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc
    59581 ggaatgacgt tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg
    59641 tcgggagaga tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg
    59701 gcgatgcagc cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt
    59761 ccggcaaatt tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca
    59821 tcgacgtaaa attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg
    59881 cgctcgttga cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga
    59941 cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc
    60001 tgtacggctt catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg
    60061 aagcggctta ccgaccagcg caatatgtcc tgaatggttt gcagctcatt tactgcttca
    60121 tcaacgaaaa ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta
    60181 gtttgccatg aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt
    60241 ttaaaacgat cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca
    60301 gataaatgaa aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga
    60361 tggcggggac tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa
    60421 tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct
    60481 ccgatgagtt tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg
    60541 tcagccattt tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg
    60601 atttacacgg tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct
    60661 gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga
    60721 agcaacaaac accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac
    60781 cgaaagagta cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gagtggttgc
    60841 cgccggagtt gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg
    60901 cctacaaata tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca
    60961 tcagagcaat actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc
    61021 aatcggttgc ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt
    61081 taccggatga cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa
    61141 ggttaacaat tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt
    61201 actgccatca cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt
    61261 tgaccccgtg gactgaattc gtaacgcctt attatggctg acataatttt cactccagga
    61321 gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt
    61381 atagcttcca aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc
    61441 ataatcatca gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt
    61501 atctatgcca gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg
    61561 agctgaaccc gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga
    61621 atcatatttc ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct
    61681 cgctacagca ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc
    61741 actcataagt ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag
    61801 cattaggctc gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg
    61861 tcactgtggc actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg
    61921 tattaaaatt taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt
    61981 tgattggcac cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg
    62041 acgaggagtc aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta
    62101 ctttgacatt aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc
    62161 gctctgactg cccaaggttc attaaatcac tggcggcaat ggcaggaaaa tgtacttctg
    62221 ccaactggct accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt
    62281 gcaaattatc tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa
    62341 acaacgtacg ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc
    62401 cgcctctggc agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc
    62461 ggtttcattc accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg
    62521 aatgcctaaa ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc
    62581 actccagctc aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga
    62641 atacggcacg gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa
    62701 agttatttca atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc
    62761 gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat
    62821 ccgtttcatt ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac
    62881 gttgtaccac tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta
    62941 aatggcaacc atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc
    63001 cgaataccaa acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt
    63061 gtcgcctgca tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag
    63121 tcaatgggac gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca
    63181 actgccgcac catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata
    63241 cttactgact tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca
    63301 gttaccgccc agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg
    63361 gctctgctct tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg
    63421 agcgccatag tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg
    63481 tgcagaggtt ttcggcgaca tcactattgg cgagaaaccg gctgccggat tgccattttt
    63541 ctgtgtgctg gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc
    63601 ctgaccgttg cgggttaaag taatcttata ttgctagggg tttttcatgc tgacgttctc
    63661 cagcgccttc ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt
    63721 ggctttgttt gattttggcg ggatctcacg cacgttgaag tagaacaggc tttcacgatc
    63781 ggcaggcaat ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga
    63841 atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc
    63901 atcttcgatc cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac
    63961 gctgattgat ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc
    64021 ctgggcagac aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc
    64081 agagcaaagt aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag
    64141 aaacaggggg attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt
    64201 catcaggaac aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa
    64261 acagatcggc aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga
    64321 acacactcat gtgttcgcca ggtttcactc cagccagata aacattgccg tcatcatcga
    64381 caaggcccac tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc
    64441 cgtcttgtaa acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt
    64501 agccaatcgc cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt
    64561 ccggcagttt gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa
    64621 cgacggcttt accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat
    64681 cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa
    64741 gtgcgccacc gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt
    64801 attgcccttc atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt
    64861 aatagccgtc aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg
    64921 cgtcatcgac acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc
    64981 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt
    65041 gtcggcctgg ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat
    65101 actgcccata ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc
    65161 ccagtaggta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
    65221 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
    65281 ggcatccaga tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa
    65341 agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc
    65401 gtccagcgaa cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac
    65461 atcaaacgcg accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg
    65521 atagttttca tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga
    65581 agcttcagca ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc
    65641 catcatgatc ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct
    65701 gatgtcatat tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga
    65761 gacagaatca ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat
    65821 cacacgcccc atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc
    65881 tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc
    65941 aacatagtta aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt
    66001 ggcttttaat gatggtaacg cccgcctggc atagtagcga ctccactccc atttttttga
    66061 gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata gttagtctgc
    66121 cagtcagcac gcatacgcca cggccctagg ttaaccccaa ccgtcccgtt gccgctgatc
    66181 tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat gctgtagtcc
    66241 gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatccctgtc gggataagta
    66301 tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa atcagcctta
    66361 atttccatgc cttccagttg accggatttc aggcatttag catcgtggct ccattgcaga
    66421 tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt cagacaagca
    66481 tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc ttccgccaat
    66541 ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata tccctggctg
    66601 gaaaaacgct tcatatcaat ttttgtgtcg ccttttaatt ccagaaaacg ggaatcaaac
    66661 tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag cgcaatgcac
    66721 cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc tatccgtttt
    66781 tattcttgtt cggtcaaaat acatttaaat cccccatagc agtgtcgcta tgggggtatg
    66841 ccttacaggt aagtaatctg gaaggtggta tttgcttcaa aattacccag atctggcgca
    66901 tcagctgcgc cgaccagcca ggcgttaaag ttcagtgtct gcttcgcttt gcctttgttg
    66961 gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta agaggtaccc
    67021 agagagtcac cgatcgccag gctaacattg ttaaatgccg caccggtatc ggtattgaaa
    67081 atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt agtggtcatt
    67141 gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt cggcgtggtg
    67201 gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc cagtttgtga
    67261 tcgtccggaa cgatagaaca cactgagtct tcaatggtgc cgtaaaaacg tgcagtaccg
    67321 ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat tgccgcagca
    67381 atagctgttt taacaaactt actcattatt tgtccttaaa ctataaataa ttaaaaatat
    67441 aaacattgca atacattgat tcagtcaata gccaatgttt atacccagcc attgaatacg
    67501 gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt cattggtgtg gggccgcata
    67561 gtgatgtttc cacacccctg atccaattta attttactaa agagtaaatg tagatttaat
    67621 taatatattg acagaagaaa ggttatctta agga
//
LOCUS       DADLEE010000008        66649 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000008 DADLEE010000000
VERSION     DADLEE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.008"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="JQH51_000559"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="JQH51_000559"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY6075022.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="JQH51_000560"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="JQH51_000560"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY6075023.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="JQH51_000561"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="JQH51_000561"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY6075024.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="JQH51_000562"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="JQH51_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY6075025.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="JQH51_000563"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="JQH51_000563"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY6075026.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="JQH51_000564"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="JQH51_000564"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY6075027.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="JQH51_000565"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="JQH51_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY6075028.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="JQH51_000566"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="JQH51_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY6075029.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="JQH51_000567"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="JQH51_000567"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY6075030.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="JQH51_000568"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="JQH51_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY6075031.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /locus_tag="JQH51_000569"
     CDS             complement(14848..15543)
                     /locus_tag="JQH51_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY6075032.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="JQH51_000570"
     CDS             complement(15666..16703)
                     /locus_tag="JQH51_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6075033.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="JQH51_000571"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="JQH51_000571"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6075034.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="JQH51_000572"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="JQH51_000572"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY6075035.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="JQH51_000573"
     CDS             19445..19885
                     /locus_tag="JQH51_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY6075036.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="JQH51_000574"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="JQH51_000574"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY6075037.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20637..21524)
                     /gene="ugpC"
                     /locus_tag="JQH51_000575"
     CDS             complement(20637..21524)
                     /gene="ugpC"
                     /locus_tag="JQH51_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY6075038.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="JQH51_000576"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="JQH51_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY6075039.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="JQH51_000577"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="JQH51_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY6075040.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="JQH51_000578"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="JQH51_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY6075041.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="JQH51_000579"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="JQH51_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY6075042.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="JQH51_000580"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="JQH51_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY6075043.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /locus_tag="JQH51_000581"
     CDS             complement(26623..27900)
                     /locus_tag="JQH51_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY6075044.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="JQH51_000582"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="JQH51_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY6075045.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livk"
                     /locus_tag="JQH51_000583"
     CDS             complement(28871..29980)
                     /gene="livk"
                     /locus_tag="JQH51_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY6075046.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="JQH51_000584"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="JQH51_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY6075047.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /locus_tag="JQH51_000585"
     CDS             complement(30986..32089)
                     /locus_tag="JQH51_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY6075048.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="JQH51_000586"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="JQH51_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY6075049.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="JQH51_000587"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="JQH51_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY6075050.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="JQH51_000588"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="JQH51_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY6075051.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36683)
                     /gene="ftsY"
                     /locus_tag="JQH51_000589"
     CDS             complement(35181..36683)
                     /gene="ftsY"
                     /locus_tag="JQH51_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY6075052.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEEAAKEEITDEELEAQ
                     ALAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFI
                     SLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKE
                     EMGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDT
                     FRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQN
                     KSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTK
                     LDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36833..37429
                     /gene="rsmD"
                     /locus_tag="JQH51_000590"
     CDS             36833..37429
                     /gene="rsmD"
                     /locus_tag="JQH51_000590"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY6075053.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37419..37688
                     /locus_tag="JQH51_000591"
     CDS             37419..37688
                     /locus_tag="JQH51_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY6075054.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37691..38050)
                     /locus_tag="JQH51_000592"
     CDS             complement(37691..38050)
                     /locus_tag="JQH51_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY6075055.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38191..38817
                     /locus_tag="JQH51_000593"
     CDS             38191..38817
                     /locus_tag="JQH51_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY6075056.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38891..41089
                     /gene="zntA"
                     /locus_tag="JQH51_000594"
     CDS             38891..41089
                     /gene="zntA"
                     /locus_tag="JQH51_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY6075057.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41191..41436)
                     /gene="tusA"
                     /locus_tag="JQH51_000595"
     CDS             complement(41191..41436)
                     /gene="tusA"
                     /locus_tag="JQH51_000595"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY6075058.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41657..42322
                     /locus_tag="JQH51_000596"
     CDS             41657..42322
                     /locus_tag="JQH51_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY6075059.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42395..42952
                     /gene="dcrB"
                     /locus_tag="JQH51_000597"
     CDS             42395..42952
                     /gene="dcrB"
                     /locus_tag="JQH51_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY6075060.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42956..44173)
                     /locus_tag="JQH51_000598"
     CDS             complement(42956..44173)
                     /locus_tag="JQH51_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6075061.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44307..45356
                     /locus_tag="JQH51_000599"
     CDS             44307..45356
                     /locus_tag="JQH51_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY6075062.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45411..45998
                     /gene="acpT"
                     /locus_tag="JQH51_000600"
     CDS             45411..45998
                     /gene="acpT"
                     /locus_tag="JQH51_000600"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY6075063.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46109..47683
                     /gene="nikA"
                     /locus_tag="JQH51_000601"
     CDS             46109..47683
                     /gene="nikA"
                     /locus_tag="JQH51_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY6075064.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47683..48627
                     /gene="nikB"
                     /locus_tag="JQH51_000602"
     CDS             47683..48627
                     /gene="nikB"
                     /locus_tag="JQH51_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY6075065.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48624..49457
                     /gene="nikC"
                     /locus_tag="JQH51_000603"
     CDS             48624..49457
                     /gene="nikC"
                     /locus_tag="JQH51_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY6075066.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49457..50221
                     /gene="nikD"
                     /locus_tag="JQH51_000604"
     CDS             49457..50221
                     /gene="nikD"
                     /locus_tag="JQH51_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY6075067.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50218..51024
                     /gene="nikE"
                     /locus_tag="JQH51_000605"
     CDS             50218..51024
                     /gene="nikE"
                     /locus_tag="JQH51_000605"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY6075068.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51030..51431
                     /gene="nikR"
                     /locus_tag="JQH51_000606"
     CDS             51030..51431
                     /gene="nikR"
                     /locus_tag="JQH51_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY6075069.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51551..51910)
                     /locus_tag="JQH51_000607"
     CDS             complement(51551..51910)
                     /locus_tag="JQH51_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY6075070.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51907..52182)
                     /locus_tag="JQH51_000608"
     CDS             complement(51907..52182)
                     /locus_tag="JQH51_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY6075071.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52241..53365)
                     /locus_tag="JQH51_000609"
     CDS             complement(52241..53365)
                     /locus_tag="JQH51_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY6075072.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53365..56100)
                     /gene="rbbA"
                     /locus_tag="JQH51_000610"
     CDS             complement(53365..56100)
                     /gene="rbbA"
                     /locus_tag="JQH51_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY6075073.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56097..57164)
                     /locus_tag="JQH51_000611"
     CDS             complement(56097..57164)
                     /locus_tag="JQH51_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6075074.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57530..59152)
                     /locus_tag="JQH51_000612"
     CDS             complement(57530..59152)
                     /locus_tag="JQH51_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY6075075.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59414..61003)
                     /locus_tag="JQH51_000613"
     CDS             complement(59414..61003)
                     /locus_tag="JQH51_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY6075076.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61499..62418
                     /locus_tag="JQH51_000614"
                     /pseudo
     CDS             61499..62418
                     /locus_tag="JQH51_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(62505..63020)
                     /locus_tag="JQH51_000615"
     CDS             complement(62505..63020)
                     /locus_tag="JQH51_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075077.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(62974..63738)
                     /locus_tag="JQH51_000616"
     CDS             complement(62974..63738)
                     /locus_tag="JQH51_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6075078.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63984..64745
                     /locus_tag="JQH51_000617"
                     /pseudo
     CDS             63984..64745
                     /locus_tag="JQH51_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64852..65364)
                     /locus_tag="JQH51_000618"
     CDS             complement(64852..65364)
                     /locus_tag="JQH51_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075079.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(65503..>66649)
                     /locus_tag="JQH51_000619"
     CDS             complement(65503..>66649)
                     /locus_tag="JQH51_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6075080.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16190 a        17891 c        16347 g        16221 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cttggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaga cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttcttcttc tgccgcttcg acaatctcta cggtttccgc ttcagcctgc
    36301 cactcttctg acgaaacctc ttcggcgttg acgtcttccg gcaacggcag ctcttcacgt
    36361 tcgatagcca ctggctccgg cgtttcttct acgaccggtt ccggctgtgc aacaacttcc
    36421 gcttcaggct gcgctttttc actttcaaca acctgttcag tgacttccac aacgtcggca
    36481 gcaaaagttt ccgcctcggc ttcagtatgc gcctgcggct gctcttcaac ggcttgttca
    36541 gaagccttcg caggctcttg cgcctgaacg atttcttcta caaccggttg ttcattctga
    36601 acttctgtct ctttttccgg ggtctgctct ttttgaccaa agcccagcca ggaaaaaaag
    36661 ccacgtcttt tttctttcgc catttgcgac tacactcctc gctgttgctt catggcacag
    36721 cgttaacgct atgtacatag cagctaaaaa aatgatgaaa tagtctatca cttaacttaa
    36781 ttcacatcac cgcctgcaag tatgtgttat ctggcggatt gagcaattta tcatgaaaaa
    36841 accgaatcat tccggcagcg gccaaatccg cattattggc gggcagtggc gaggccgtaa
    36901 actcccggtt cctgatagcc caggtctgcg ccccaccacc gaccgcgtac gcgaaacgtt
    36961 gtttaactgg ctggctccgg tcattgttga cgcccaatgt ctggattgct tcgccgggag
    37021 cggcgcgctg gggctggaag cgttatcgcg ctacgctgcg ggggcaacgt tgattgagat
    37081 ggatcgcgtg gtttctcagc agttaattaa gaatctggcg acactaaaag caggcaatgc
    37141 acgcgtggtg aacagcaacg caatgtcatt cctggcgcaa aaaggcacac cgcataatat
    37201 cgtgtttgtc gatccaccgt tccgccgtgg cttgttagaa gagacgataa atttactgga
    37261 agataacggc tggctggctg acgaagccct gatttatgtc gaaagcgaag tcgaaaacgg
    37321 tctgcccact gttccggcaa actggtcatt acatcgggaa aaagtggcgg gtcaggtggc
    37381 ttatcggctg tatcaacacg aagcacaagg agaaagtgat gctgattaat attggtcgtt
    37441 tgttaatgct ctgcgtttgg ggatttttaa tcctcaatct ggtgcatccc ttcccgcgcc
    37501 cgctaaatat tttcgttaac gtggcgctga tttttaccgt gctgatgcat ggtatgcagc
    37561 tggcgctatt gaaatccact ttaccgaaag atggcccgca gatgaccacc gccgaaaagg
    37621 tgcggatttt ccttttcggc gtgtttgaac tgctggtctg gcagaagaaa tttaaagtta
    37681 aaaaataacc ttattgttcg ccaacaaagc taacaaagcg cgttccttta tagctcagcg
    37741 tacctttatc gcccactgtc agggcgtggt actgctgggc gtcgaggcga aacgtctgct
    37801 ccattcctcc gctttgcggt ttgaagcttg cctcatagcg catactggtg cctgccggag
    37861 tcacttcctg ctggcgcgaa cggcgatcgt taatcggttt ttcccgcttg ttgctcacca
    37921 ccaccagctt ttgctggagc ggagccatat cattatcagc tttttcccgt cgctgttgca
    37981 taaaacgaaa cgatgcggcg acgacaatta agccaatgat aacaataaag aaaagaggtg
    38041 gtttactcat ctttatccct catcggaaaa tgcggaatta agcataccct gccagttatg
    38101 gtgttgtcat ccgtccaccc tcgccactaa actggaagca agaccgtagg cattctgctt
    38161 acgaaaaaat aacgaattca aggaactaag atgctttggt cgtttatcgc tgtctgtctt
    38221 tccgcatggc tatctgtgga tgcatcgtat cgtgggccaa cctggcaacg ctgggtgttt
    38281 aaaccgttaa cccttcttct cctgctgtta ctggcctggc aagcaccgat gttcgacgcc
    38341 attagctatc tggtgctggc gggactgtgc gcctcactgc tgggcgatgc gctaaccctg
    38401 ttgccacgtc aacgtctgat gtacgccatc ggcgcgtttt tcctctcgca cctgctgtac
    38461 accatctatt tcgccagtca gatgacgctc tctttcttct ggcctctgcc actggtgttg
    38521 ctggttctgg gtgcgctgct actggcgatt atctggacgc ggctggaaga gtaccgttgg
    38581 cctatctgca cgtttatcgg catgacgctg gtgatggtgt ggctggcagg tgaactgtgg
    38641 ttcttccgtc cgaccgctcc ggcgctctct gcgtttgttg gcgcttcgtt gctgtttatc
    38701 agtaactttg tctggctggg gagccactat cgccgacgct tccgtgcgga taacgcgatt
    38761 gctgcggcct gctactttgc cggtcacttc ctcatcgtcc gctcactgta tctctgataa
    38821 aacttgactc tggagtcgac tccagagtgt atccttcggt taatgagaaa aaacttaacc
    38881 ggaggatgcc atgtcgacac ctgacaatca cggcaagaaa gcccctcaat ttgctgcgtt
    38941 caaaccgcta accacggtac agaacgccaa cgactgttgc tgcgacggcg catgctccag
    39001 ctcgccaacg ctctctgaaa acgtctccgg cacccgctat agctggaaag tcagcggcat
    39061 ggactgcgcc gcctgtgcgc gcaaggtaga aaatgccgtg cgccagcttg caggcgtgaa
    39121 tcaggtgcag gtgttgttcg ccaccgaaaa actggtggtt gatgccgaca atgacatccg
    39181 tgcccaggtt gaatctgcgg tgcaaaaagc gggctattcc ctgcgcgatg agcaggccgc
    39241 cgacgagcca caagcatcgc gcctgaaaga gaatctgccg ctgattacgc ttatcgtgat
    39301 gatggcaatc agctggggtc tggagcagtt caatcatccg ttcgggcaac tggcgtttat
    39361 cgcgaccacg ctagttgggc tgtacccgat tgctcgtcag gcattacggc tgatcaaatc
    39421 cggcagctac ttcgccattg aaaccttaat gagcgtagcc gctattggtg cactgtttat
    39481 tggcgcaacg gctgaagctg cgatggtgtt gctgctgttt ttgattggtg aacgactgga
    39541 aggctgggcc gccagccgcg cgcgtcaggg cgttagcgcg ttaatggcgc tgaaaccaga
    39601 aaccgccacg cgcctgcgta acggtgagcg ggaagaggtg gcgattaaca gcctgcggcc
    39661 tggcgatgtg attgaagtcg ccgcaggtgg gcgtttgcct gccgacggta aactgctctc
    39721 accgtttgcc agttttgatg aaagcgccct aaccggagaa tccattccgg tggagcgcgc
    39781 gacgggcgat aaagttcctg caggagccac cagcgtagac cgtctggtaa cgctggaagt
    39841 gctgtcagaa ccgggtgcca gcgccattga ccggattctg aaactgatcg aagaagccga
    39901 agagcgccgc gcccccattg agcggtttat cgaccgtttc agccgtattt atacgccagc
    39961 gattatggcc gtcgccctgc tggtaacgct agtgccgccg ctgctgtttg ccgccagctg
    40021 gcaggagtgg atttataaag gtctgacgct gctgctgatt ggttgcccgt gtgcgttagt
    40081 tatctcaacg cctgcggcga taacctccgg gctggcagca gcagcgcgtc gtggggcgtt
    40141 aattaaaggc ggtgcggcgc tggaacaact aggtcgtgtt acccaagtgg cgtttgataa
    40201 aaccggtacg ctgaccgtcg gtaaaccgcg cgttaccgcg attcatccgg caacgggtat
    40261 tagtgaatct gaactgctga cactggcggc ggcggtcgag caaggcgcga cgcatccact
    40321 ggcgcaagcc atcgtacgcg aagcacaggt tgctgaactc gccattccca ccgccgaatc
    40381 acagcgggca ctggtcgggt ctggcattga agcgcaggtt aatggtgagc gcgtgttgat
    40441 atgcgctgcc gggaaacatc ccgctgatgc atttgctggt ttgattaatg aactggaaag
    40501 cgccgggcaa acggttgtgc tggtagtacg taatgatgac gtgctgggta tcattgcatt
    40561 gcaggatacc ctgcgcgccg acgctacaac tgccatcagt gaactgaacg cgctgggggt
    40621 taaaggagtg atcctcaccg gcgataatcc acgcgcagcg gcggcaattg ccggggagct
    40681 ggggctggag tttaaagcgg gcctattgcc ggaagataaa gtcaaagcgg tgaccaagct
    40741 gaatcaacat gcgcccctgg cgatggtcgg tgatggtatt aacgacgcgc cagcgatgaa
    40801 agctgccgcc atcgggattg ctatgggtag cggcacagac gtggcgctgg aaaccgccga
    40861 cgcagcatta acccataacc acctgcgcgg cctggtgcaa atgattgaac tggcacgcgc
    40921 cactcacgcc aatatccgcc agaacatcac cattgcgctg ggactgaaag ggatcttcct
    40981 cgtcaccacg cttttaggga tgaccgggtt gtggctggca gtgctggcag atacgggtgc
    41041 gacggtgctg gtgacggcga atgcgttaag gttgttgcgc aggagataag gcaaaccgat
    41101 cgcaacattg agcgcgatcg gtcccctcgc ccctctgggg agagggttag ggggtgagga
    41161 aaaggcggca tcgaagccaa tcagccccta tcaaccgcct ttacgaatca aataacgata
    41221 aggcagtcca tccgtctctt tcgcaaccag ttcgtgttcc ataaaggtac aaaacccagg
    41281 aatatcgcgg gtagtggccg gatcgtcggc gataatcagc aacgtttcgc caggctgcat
    41341 attgcgcacg gttttgcgca ccatcatcac cggttccggg cagcgcaggc caagcgcgtc
    41401 gagtgtgtgg tcagggctgg aaaagagatc ggtcattttc ttctcatcac ttaaaaaaac
    41461 ggcgctagtt tacgccctgt gagtccgtaa gcaaaccagg ttaacgattg cgtgaaaatt
    41521 aaccattgca ttgtcaacgt aaagcagtat catgcggcgg ctcgaaaaaa gggtaagcac
    41581 gttattatgt taaggtaaca gacgtgtcgt acgtattggg ttccctcacc ccaatggtta
    41641 atcaaaaagg tacaatatga acgttttctc gcaaactcaa cgctataagg cgttgttctg
    41701 gttatcgtta tttcatcttc tggtgatcac ctccagtaac tatctggttc agcttcccgt
    41761 ctccattttt ggtttccata ccacctgggg cgcgtttagc tttccgttta tttttcttgc
    41821 taccgacctg accgtgcgta tttttggcgc accgctggcc cgacgcatta tcttcgcggt
    41881 aatgatccct gcgttattaa tctcctacgt catctcgtcg ctattctata tgggttcctg
    41941 gcagggattc ggcgcactcg cccacttcaa cctgtttgtc gcccgtatcg ccaccgccag
    42001 tttcatggcc tacgcgctgg ggcaaatcct cgacgtgcac gtttttaacc gcctgcgtca
    42061 gagtcgccgc tggtggctgg caccgacagc gtccacactg ttcggtaacg tcagcgacac
    42121 gctggccttt ttcttcattg ccttctggcg tagcccggat gcctttatgg ctgaacactg
    42181 gatggaaatc gcgctggtcg attactgttt caaagtgtta atcagtatcg ttttcttcct
    42241 gccaatgtat ggcgtattac tcaatatgct gttgaaaaga ctggcagata aatccgaaat
    42301 caacgctttg caggcgagtt aaaggttcgt tatcagagtt gtgataagat ggatgaatga
    42361 gccgttatgg ccgtttatcg aaaggaagaa gtcaatgcgc aatctggtta aatatgtcgg
    42421 aattggcctg ctggttatgg ggcttgcggc ctgtgatgat aaagacacta acgctacggc
    42481 gcagggttcg gttgcggaaa gtaacgctac cgggaatccc gtcaacctgc ttgatggcaa
    42541 gttaagtttc tcgctgccag cggatatgac cgaccagagc ggtaagctgg gaacgcaggc
    42601 caataacatg catgtctggt ccgacgccac cgggcagaaa gcagtcatcg tcatcatggg
    42661 cgatgatccg aaagaagatc tggcggtgtt ggcgaagcgt ctggaagatc agcaacgtag
    42721 ccgcgatccg cagctgcaag tggtaaccaa taaagccatt gagctgaaag gtcacaaaat
    42781 gcagcagtta gacagtatta tctccgcgaa aggccagacg gcgtactctt ccgttattct
    42841 gggtaacgtg ggtaatcaac tgctgaccat gcaaattacg ctgccagctg acgatcagca
    42901 aaaagcgcaa accaccgcag aaaacatcat taatacgctg gttattcagt aagttttaag
    42961 atgatgaggc ggcctcaggg acgtgttccg gaggccgttt ttttaatcgc cacgtcagta
    43021 ataacgcgat tgcgaccagt cccgccgccg ccagataaat caccggtacg cccgcccagc
    43081 tcatcaccag cccagccagt ggtccagtca cgccaagcga taaatccata aatacggtgt
    43141 aagttgccag cgccgccccc tgattttgct gcggaaccgc ttttaccgcc actacaccca
    43201 atgccgggaa caccagcgaa aacccggccc ccgccagtaa gacgccgatt ttcgccatcc
    43261 acggcatagt cgccacgcca accagtagca ggccgattat ctcaacgcta aagcaaatca
    43321 tcgctacgtt taagccaccg atacggttaa tgccgttagg gaataacaaa cgcgtaccga
    43381 caaacgcaca gctaaacagc gtcagcgcga aagccgcacc gtcccaacct ttagcgtcat
    43441 aaaacagcgt gataaaggtg gcgatgacgc caaatccggc ggaagccagt gccagcgcca
    43501 taccgtacag ccagacgcgc ccaagcaccg cgcgaaacgg cagcggtttg cctttactgg
    43561 cttttaccgt cggacgcggg atcgccaaca aaatggccac cagcgccacg cccataatga
    43621 ttaacgctaa cgcctgcaag ccgccccagt gataaaacac gacgcctaac ggcgcaccca
    43681 tcgccatcgc cccgtaagtg acaatgccgt tccacgaaat cacccgcccg atatgcagcg
    43741 agccaaccac tccaacgccc catagggtcg atcccgttcc ggcaaaactt tgcccaatcc
    43801 caaggatgac gcgccccagg caaagtaata acaggctgat gacaggcaga ctggcggtta
    43861 atcctgccgt cagatacccc agaccgctca aaaagcagcc gcataaaccg aagacgacaa
    43921 tctttttggg tcccagcaaa tcggcgtaac gtccggcatg agggcggctc agcaaggtgg
    43981 cgaaatattg caggctgata accaatcctg cccagaaggc gctaaagccc atcacatcat
    44041 ggacatagcc cggtaataca gcgagcggca acccgatggt gaggtagctg gcgaagttaa
    44101 acatgacaat ggagacaatg cgcaaattca ggcgcaatcc gtttagcgcg ggttcggcta
    44161 cgggttcggg catgaggatc accacatttt tacaacagtg tttcattttt accatgtgct
    44221 gacgtgaaaa tcagcagtaa gaatcagaat attgctggcg tgactcccgc gctacactta
    44281 acacaaaaag tcacaaggaa gccccaatgg aaacccctca acccgataaa acgggcatgc
    44341 acattctgct caagttggcc tcgctggtag tgatcctcgc gggcattcac gcagcggcag
    44401 atatcattgt gcagctgtta ctggcgctgt tttttgccat cgtcctcaac ccgctcgtta
    44461 cctggtttat tcgtcgggga gtacaacgcc ccgttgccat tacgattgta gtggtggtga
    44521 tgctgatcgc actaaccgcg ctggtcggcg tactggcggc atcgtttaac gaatttatct
    44581 ctatgctgcc gaagtttaat aaggagctga cgcgcaaact ttttaaattg caggagatgt
    44641 tgccttttct taatttgcat atgtcgccgg agcgaatgct gcagcggatg gactcggaaa
    44701 aagtggttac cttcaccaca gcgctaatga ccgggctttc cggggcaatg gcgagcgtgc
    44761 ttttgctggt gatgaccgta gtttttatgc tgtttgaagt gcgccacgtc ccttacaaaa
    44821 tgcgttttgc gctgaataac ccacagattc acatcgcagg attacaccgc gcacttaaag
    44881 gcgtttcgca ttatctggcg ttaaagacgc tactcagttt atggacaggt gtcatcgtct
    44941 ggctggggct ggcgctaatg ggcgtacagt ttgcgctgat gtgggcagta ctggcgtttt
    45001 tgctcaacta cgtgcccaat atcggcgcgg taatttccgc cgtaccgcca atgattcagg
    45061 tgctgctgtt taatggtgtt tacgaatgta ttctggtcgg cgcattgttt ttagtggtcc
    45121 atatggtcat cggcaatatt ttagaaccac ggatgatggg ccatcgcctg gggatgtcca
    45181 ccatggtggt atttctttca ttgttaatct ggggatggct gctcggcccg gtagggatgc
    45241 tactttcggt gccattaacc agcgtgtgta aaatctggat ggaaacgacg aaaggtggca
    45301 gcaaactggc gattttactg gggccgggca gaccgaaaag tcggttaccg ggatgaggcg
    45361 acaagtgata cgatacgcac tttcattttc cattaaacgt tggccctgat atgtatcgga
    45421 tagttctggg gaaagtttcg accttaagcg cagctccact gccaccgggt ttacgcgagc
    45481 aagcaccgca aggtccacga cgcgaacgct ggctggcggg gcgtacattg ctttcgcaca
    45541 cgctttcccc gctaccggag atcatctatg gcgaacaagg caaacctgca ttcgcgccgg
    45601 aaacgccgct atggttcaac ttaagccata gcggtgacga tatcgctctg ctgttgagcg
    45661 atgaaggcga agtcggctgc gatatcgaag tgattcgccc gcgcgtcaac tggcgctggc
    45721 tggcgaacgc cgtgttcagc ctcggggaac acgctgaaat ggacgccgtg catcctgatc
    45781 agcaactgga aatgttctgg cgcatctgga cgcgcaaaga agccatcgtt aaacagcgtg
    45841 gcggcagcgc ctggcaaatc gtcagcgtag atagcaccta tcactcctcg ctgtcagtca
    45901 gccattgtca gctagaaaat ttaagccttg cgatctgcac ccccaccccc tttacgctca
    45961 ccgccgacag tgtgcaatgg atcgattcag ttaactgatc cgcccacccg actgcccatc
    46021 tattgatcca gaacaggtaa tcagtatgac gaatacttaa aatcgtcata cttatttccg
    46081 ccatctattt taatccattg gggttaccat gctctccaca ctccgccgca ctctatttgc
    46141 gctgctggct tgtgcgtctt ttatcgtcca tgccgctgca ccagatgaaa tcaccaccgc
    46201 ctggccggtg aatgtcgggc cactaaaccc gcacctttac acgcctaacc agatgttcgc
    46261 ccagagcatg gtttatgaac cattggtgaa atatcaggca gacggttcgg tgatcccgtg
    46321 gctggcaaaa agctggactc attcagaaga tggtaaaacc tggaccttca ccctgcgtga
    46381 tgacgtgaaa ttctccaacg gtgaaccgtt cgatgccgag gcggcggcag aaaacttccg
    46441 cgcagtgctc gataaccgtc aacgtcacgc ctggctggag ctggcaaacc agattgttga
    46501 tgttaaagca ctcaataaaa cagagctgca aattaccctg aaaagcgcct actatccttt
    46561 cctgcaagaa ctggccctgc cccgtccttt ccgttttatc gctccctcgc agtttaaaaa
    46621 ccatgaaacc atgaacggaa ttaaagcgcc gattggcacc ggaccgtgga ttttgcagga
    46681 atcgaaacta aatcagtacg atgtcttcgt ccgtaacgaa aactactggg gcgaaaagcc
    46741 agcgattaaa aagatcacct ttaacgtcat cccggacccg actacccgcg cggtggcgtt
    46801 tgaaactggc gatatcgacc tgctgtacgg aaacgaaggg ttattaccgc tcgatacctt
    46861 cgcccgcttt agccagaatc cggcttacca cacccaactg tcacagccga tcgaaaccgt
    46921 gatgctggcg ctcaataccg ccaaagcccc caccaacgag ctggcagtac gtgaagctct
    46981 taattacgcg gtaaacaaaa aatcgctgat tgataacgcg ttatatggca cccagcaggt
    47041 cgccgacacc ctgtttgccc cttctgtgcc ttacgctaat ctcggcctga aaccgcgcca
    47101 gtacgatccg cagaaagcga aagagttgct ggaaaaagcc ggttggacgc tgcctgcggg
    47161 caaagacatc cgcgagaaaa atggtcagcc gctgcgcatt gaactgtcgt tcatcggcac
    47221 cgatgcgtta agcaaatcga tggcggaaat cattcaggct gatatgcgcc agattggcgc
    47281 agatgtctcg ctgattggcg aagaagagag cagtatctat gctcgtcagc gcgacggtcg
    47341 ttttggcatg attttccacc gcacctgggg cgcgccatac gatccacacg ccttcctgag
    47401 ttcaatgcgc gtaccgtcgc acgctgactt ccaggcacag caaggattag ccgacaagcc
    47461 gctgattgat aaagagatcg gcgaagtgct ggcgacccat gacgaaacgc aacgtcaggc
    47521 gctgtatcgc gacattctga cccgtctgca tgacgaggcg gtttatctgc ctatcagcta
    47581 catctcgatg atggtggtat caaaaccgga gctgggtaat atcccctacg cgccgatcgc
    47641 caccgaaatt ccgttcgaac agattaaacc ggtgaaacct taatgttgcg ttacgtatta
    47701 cgccgctttc tgctgctgat cccgatggtg ctcgccgcct cggtgatcat ttttctgatg
    47761 ctgcgcctcg gtaccggcga cccggcgctc gattatctgc gtttatctaa cctgccgccg
    47821 acgccggaga tgctggcctc tacccgcacc atgctcgggc tggatcagcc gctgtacgtc
    47881 cagtacggta cctggttgtg gaaggcgctg catcttgact ttggtatctc attcgccagc
    47941 caacgcccgg tactggacga tatgctgaac ttcctgcccg ccacgctgga acttgcaggt
    48001 gcggcgctgg tattaattct gctcacttcc gtaccgctcg gtatctgggc ggcgcgccac
    48061 cgcgatcgtt tgccggattt cgccgtgcgc tttatcgcgt ttcttggcgt gtcgatgccc
    48121 aacttctggc tggcattttt gctagtgatg gcgttttcgg tgtatctgca atggctaccc
    48181 gcgatgggct acggcagctg gcagcacatc attttacctg cggtttccat cgcctttatg
    48241 tcgctggcga ttaacgcgcg actgctgcgc gccagtatgc tggacgtcgc cggtcagcgt
    48301 cacgtcacct gggcgcggct acgcggcctg aatgacaaac agaccgaacg ccgccatatt
    48361 ctgcgcaatg cctcgctgcc gatgatcacc gccgtgggga tgcacattgg cgaattgatt
    48421 ggcgggacga tgattatcga aaacatcttt gcctggcctg gcgtcgggcg ctatgcggtg
    48481 tcggcgattt ttaaccgtga ctatccggtg attcagtgct ttacgctgat gatggtggtg
    48541 gtttttgtgg tctgtaattt gattgtcgat ttgctcaacg ccgcgctgga cccgcgcatt
    48601 cgtcgtcatg aaggagcgca cgcgtgaact ttttcctctc ttcccgctgg tcggtacgcc
    48661 tggcgctgat cattatcgcc ctgctggcgc tgattgcgct caccagccag tggtggcagc
    48721 cgtacgatcc acaggcaatt gatttgccgt cgtgcctgct ttcgccggat gcgcagcact
    48781 ggctgggcac cgatcactta ggtcgcgata ttttctcgcg gctgatggca gcgacccgcg
    48841 tgtcgctcgg ttcggtaatg gcctgcctgc tgctggtgct gacattaggg ctggttattg
    48901 gcggcagcgc cgggttgatt ggcgggcacg ttgatcaggc caccatgcgc gtcgccgata
    48961 tgtttatgac cttcccgacc tcgattctgt cgttctttat ggttggcgtg ctcggcaccg
    49021 ggctgaccaa cgtaattatc gccatcgccc tgtcgcactg ggcgtggtat gcacgcatgg
    49081 tgcgcagctt ggtgatttca ctacgccaac gcgagtttgt gctggcgtca cggctttccg
    49141 gtgcgggcca tgtgcgggtg tttgtcgatc atctggcagg cgcggtgatc ccttcgctgc
    49201 tggtgctggc aacgctggat atcggccata tgatgctgca cgtcgcgggg atgtctttcc
    49261 ttggcctcgg tgtgaccgcg ccgaccgccg aatggggcgt gatgattaac gacgcgcgcc
    49321 agtatatctg gacccagccg ctgcaaatgt tctggccggg gctggcgctg tttatcagcg
    49381 tgatggcctt taacctggtg ggtgacgcac tgcgcgatca tctggatcct catctggtga
    49441 cggagcacgc acactaatgc cgcaacagat tgaactacgt aatatcacgc tacaggccgc
    49501 gcagccgctg gtacacggtg tatcgttaac cctgcaacgc gggcgcgtgc tggcgttagt
    49561 cggcggtagc ggcagcggga aatcattaac ctgcgccgcg acgctgggca ttttgcccgc
    49621 tggcgttcgc cagacggcgg gggaaatttt agccgatggc aaaccggttt cgccttacgc
    49681 cctgcgcggc atcaaaattg ccaccatcat gcagaacccg cgcagcgcct ttaatccact
    49741 gcacaccatg cacacccacg cgcgggaaac ctgcctggcg ttagggaaac ccgccgatga
    49801 cgctacgctt accgctgcca tagaagcggt ggggctggaa aacgccgcgc gcgtgctgaa
    49861 gctgtacccg ttcgagatga gcggcggcat gttgcagcgc atgatgattg cgatggcggt
    49921 gctgtgtgaa tcaccgttta tcatcgccga tgaaccgacc accgacctcg acgtggtagc
    49981 acaggcgcgc atcctcgatc tgctggaaag cattatgcaa aaacaagcgc cgggaatgct
    50041 gctggtcacc catgatatgg gcgttgtggc gcgtctggcg gatgacgtgg cggtgatgtc
    50101 acaaggtaaa attgtcgaac agggcgatgt agaaacgctg tttaacgccc ccaaacatac
    50161 ggtgacgcgc agcctggttt ccgctcatct cgccctctac ggtatggagc tggcatcatg
    50221 accttactta acgtctgcgg cctttcccat cactatgccc acggtggatt tagcggaaaa
    50281 catcaacatc aggcggtgct gaataacgtt tccctgaccc tgaaaagcgg cgaaaccgtc
    50341 gccctgctgg gacgcagcgg ctgcggaaaa agcaccctcg cgcggctgct ggtaggctta
    50401 gagtctccct cacaagggaa tattagctgg cacggcgaat cgctggcgaa acttaatcgc
    50461 gcccaacgta aagccttccg ccgtgatatt cagatggtgt ttcaggactc catcagtgcc
    50521 gtgaatccgc gcaaaaccgt gcgcgagatc ctgcgtgaac cgatgcgcca cctgctatca
    50581 ctgaaaaaat ccgaacaact ggcgcgcgcc agcgaaatgc tgaaggcggt cgatctcgat
    50641 gacagcgttc tcgacaaacg cccaccgcag ttaagcggcg gccagctcca gctcgtctgc
    50701 ctggcacgcg cgctggcggt cgaaccgaaa ttgctgattc tggatgaagc cgtttctaac
    50761 cttgatctcg tgttacaggc gggcgtcatt cgcctgctga aaaagctaca acaacagttt
    50821 ggcactgcct gcctgttcat cactcatgac ttacgcctgg tggaacgctt ttgccagcga
    50881 gtgatggtga tggacaacgg acaaatcgcc gaaacccagg cggtgggaga taaattaacc
    50941 ttttcctctg acgccggacg tgtgctacaa aacgcggtat tacccgcatt ccccgtgcgc
    51001 cgtcgcacct cagaaaaggt ttaacgccaa tgcaacgagt caccatcacg cttgatgacg
    51061 atttactgga gacgctggac agcctgagcc agcgtcgtgg ttataacaac cgttccgaag
    51121 ctatccgcga tattctgcgt agtgccctgg cgcaggaagc tacccagcag cacggcacac
    51181 aaggtttcgc ggtgctgtcg tatgtgtatg aacacgaaaa acgcgactta gccagccgta
    51241 ttgtttccac ccagcatcat caccacgacc tctccgtcgc cacgctacat gtgcacatca
    51301 accacgacga ctgtctggaa atcgccgtgt tgaaaggtga catgggtgac gtgcagcatt
    51361 ttgccgatga cgttatcgcc cagcgcggcg tgcggcacgg gcatttgcag tgcctgccga
    51421 aggaagattg agttatttct aaaacaagga aagaaaagat attaactgaa cttaatttat
    51481 aaaattaagt tcagttgcat agatagatcc tgtaaaggca gatattctgc gtaatgtacg
    51541 tcgctaaaat ttataaatga ttcaaacgct cattcagtgt ttcaataata taggtattaa
    51601 cagagacttg ttgctgcgca gccgcattat taaggcgttc actcagcgat tctggatagc
    51661 gtaacgtaaa ggtcttaatc ttttctgtac gcgcatacgg ctcaataccc gcagctttac
    51721 aatcctcaag atactctcgc agggaaagct ctccttcttt ttgcagcccc tgaatactgt
    51781 cagagacaaa atcacaataa cctgacaagc ccaggaattt gccacgaaat gcattcagtt
    51841 ctggcacgta ggtaataaca gctggttgcc cggcaatctc catagagttt ggcgtcttca
    51901 gtttgatcat ggttttaccc ctatacttag taaccagtca cacacgcttt ctacagcgcc
    51961 tttatcggta tctggcgacg gatgaggccg atggaaacac gcaacgctca tatttagtat
    52021 gaacttgcaa cgcgaacctc ttccttcctt aatttctccg cctaatgctt taaccagtga
    52081 ctctatatca gaccacttga tcccttgagg aacaggagtt ttaaatattt gatccaatgt
    52141 atttttttgt ttcttccgta aagcctgtac cttggttctc atggcatgtc cttatgactt
    52201 cactttatga catcacagta tgaagtcaag tttcccttcc ttacgccatt gtcccaatcg
    52261 tcttcctgaa tcgcagcagt gcgatggtga aaaacgcgcc gccaattgcc atcagcgtca
    52321 ggaactgcgg ccagacgatt tcgaatccgg caccccggta gaggatggcc tgcgcgaggc
    52381 taacaaagtg tgtcgtcggc atggtcagca taatgtcctg caccatctgc ggcatacttt
    52441 cgcgcggcgt ggatccaccg gaaagcattt gcagcggcag cagcaccaga atcaccagca
    52501 gccccagttg cggcattgaa cgcgctatcg tccccataaa aatgccgatt gacgtggtgg
    52561 caaacagact gagcgccacg cccagcataa acagcgggat cgagccttca atcggtacgc
    52621 ccagtacacc tttcaccatc agcaccagcg ataatcccga taccaccagc accaccagcc
    52681 ccatcgacca gatcttcgcc atcatgatct caaacggcgt tatcggcatc accagtaaat
    52741 gttccaccgt gccgtgttca cgctcgcgga tcagcgccga tccggtcaat acaatcgcca
    52801 gcatggtaat gttgttgatg atcgccatca ccccgccaaa ccacgcggga tcaaggttcg
    52861 ggttaaagcg catccgggtt tccagcgata ccaacggttc gctgttatca cggtagcgcg
    52921 cgacaaagct gttcacttca ccgttgataa tattctggat atacccattg ccggtaaatg
    52981 cctggctcat gcgcgtggca tcgacgttca cctgaatatc cggctggcgt ccggcgagga
    53041 catcacgctg aaaattaggc ggaatattta tcgcgaaggt atagcgtccg gcgtccagtc
    53101 cggcatccat ctcatcggcg gtgatcatct ccggtggcaa aaaccacgga cgatagaagc
    53161 tgttaacgat ccggttcgat aactgcgatt gatccatatc ggcaatggcg atcggcgcga
    53221 ggttcaacga tcctggcgta acggtcgctg acgaatacac cgacaccgta aacgagaaga
    53281 caatcagcgt cagcatcgct ttatcaccga gcagactgcg caactctttg atacccagat
    53341 taaaaatatt gcgtaaatgg cgcatcatcc ctcctgtttt ttcagcagca ggatgcttaa
    53401 gcccatcacc agcgggatgg ctatcagcaa cgggataaaa agttgccaca aatcagtcag
    53461 atccagcgct ttcgagaacg ttccgcgggc gatagtcaga aaatgactgg tcgggtaaac
    53521 ctcgccgatc caacgtccag gcccttccag cgaagctacc ggatcgatca tcccggaaaa
    53581 ctgtgtcgcc gggatcaatg tgataatcgc cgttccgaaa atggcggcaa tctggctttt
    53641 cataaaggtg gagatcagca gccccattcc agtggcaatg atgatataca gcagcgccgc
    53701 caaggtgagc gtcaggaaac tgcctttatg cggtacgcca aacacaaaca ccgacaggcc
    53761 gcagagcagg aaaaagttca gcatccccag cgcgatgtac ggcagctgtt tgccaagcaa
    53821 aaattcgcta cgggtggtgg gggtcacgta aaggttgata atcgacccta gctctttttc
    53881 ccgcaccacg ctaagggcgc ttagcattga cgggatcatc atcagcagaa gcgggatcac
    53941 cgccggaaca atcgctggca ggctttttac gtccgggtta tagcgataac gcgtctcaat
    54001 gttcatcagc ccgctttggc tggcgggtgt cgattgtcgg ctcgccacat cctgaagcca
    54061 actctggtgc attgcctgca cgtaaccttt taccgtttcg gcacgactgg gcatcgctcc
    54121 gtcgatccag acgccgagtt ccacaggcgt accacgcgcg atatcgcgcc cgaaattggg
    54181 cgggatctca atggccaccg tgatatcgcc cgcacgcatc cgacgatcaa gctcgtcata
    54241 actggtgagc ggcggctgtt cgataaagta acgggaaccg gagaggttga gtgtccacgc
    54301 ctggctactg acggtctggt cgcggtcgag caccgcaaag cgcaggtttt ccacatccat
    54361 actgatgccg taacccatta tcagcatcag gatcaccgtt cccatcagcg ccagcgtcga
    54421 acgtactgga tcgcgtcgca gttccagcgc ttcgcggcgg ctgtagctaa acagacggcg
    54481 caggctaaat ccctgacgcg gcgcgtgggt ggtgtcgtgt accacgggcg gcgcttcggc
    54541 ttcgttgctc tgccctgccg cttcctgcaa ataggcgata aatgcctctt ccagactggc
    54601 ggctccgcgt ttctcaacca gttcctgcgg tgtaccgctg gcaagcactt ttccggcgtg
    54661 catcagtgag atgcggtcgc aacgttccgc ttcgttcata aagtgggtgg agatgaagat
    54721 agtcactttg tcctggcgcg agagatcgac catcaactgc cagaacatat ccctcgccac
    54781 cggatcgaca ccagaagtag gctcatcgag gattaacatc tccgggcgat gaatcaccgc
    54841 caccgccagc gaaagccgct ggcgaatgcc gagcggcaat gactccggca gaacatcttc
    54901 aacgtcgttg agcttaaaac gctcgctcat ttcagccact cgcgcgggaa tttccgcttc
    54961 cgggatgtga aacaaacggg catgtaactc caggttttgc cgcacggtga gttcgttata
    55021 gagcgaaaac gcctgcgaca tatagcccac acgacggcgg gtatcgatat cttttggatc
    55081 aaccggttgc ccaaatagcc acgcctcacc ttcgctggcg ggcagcagtc cggtaagcat
    55141 tttcatggtg gtggatttac cgcagccgtt cgaaccaagg aatccaaaaa tctccccgcg
    55201 tggaatgcgg aaattgacgt gatcaacggc aacgaaggaa ccaaaacgca tggtcagatc
    55261 gcgcgcttcg atggcaatct ctgcgttttc aggttgatac ggtgggatca ctaccgcctg
    55321 atgcgcctgg cgttgcgctt gcggtaacag attaataaat gcttcttcca gcgtggcgct
    55381 ttgcgtttgc tgccgcagct cttcggcgct gccggttgcc agcacttctc cggcattcat
    55441 cgctaccagc cagtcgaagc gttcggcctc ttccatatag gcggtggcga ccagcacgct
    55501 catattgctc tgccgctggc gaatactgtc gatcagatcc cagaactggg cgcgggagag
    55561 cgggtcaacc cccgttgttg gctcatcaag gatcaacagt tccgggtcgt ggattaacgc
    55621 gcagcacagc ccaagttttt gcttcatccc gccggagagt ttacctgccg gacgatcgcg
    55681 aaacggtgct aacccggtgc tggtcagcag ctcattaatt cgcgcttccc gctccgcttt
    55741 atcgtgaccg aacaggcgag cgaaaaaatc gacattttca tacaccgaca aggtgtggta
    55801 gaggtttttg cccagcccct gcggcatcca ggcgatgcgc gggcagacgt ctcggcgatg
    55861 cttcgggtcg tgcatatcgc cacccagcac caccacattg ccctgctcaa tgacgcgggc
    55921 accggaaatc agtgacaata aactcgactt cccgacgcca tccggaccaa tcagcccgac
    55981 catgcaacgg gccggaatat cgagagtgat attgttcagc gcaacggttt ttccataatg
    56041 ctggctcacg cccgccagtt gcgcgacagg cgggacggga accagttcca gatgcgtcat
    56101 tgcggcaacc tcaccacgag gtcgtcaggc cacggaagtt cttcattcac ccgcacccac
    56161 gctacgcccg gtaaaccggt tttgacatat tccagatgct gctggagtaa ttccggtggg
    56221 atacgcgctt tgacgcggaa catcagtttc agccgttcat cgctggtttc gacggttttt
    56281 ggcgtgaact gggcgacact ggcgacaaaa ctgatggttg caggaatacg cagatctggc
    56341 gcggcatcga ggatcagccg ggcttcaccg cccagtttca gcgtgcccgc ctgttcggtt
    56401 ggcaggaaga aggtcatata gacgtcgctg agatcgacca tattcagcac ccgaccgcct
    56461 gccgccagca cttcccctgg ctcggcaacc cgatactgca cgcgtccgtc acgcggggct
    56521 ttcagttcac tgtcatcgat atctgcggca atgcgccgtt cagtggcttg cgctgcttcg
    56581 acccgagttt gcgcctgaat gatattggtg cgtgctgctt ctatagccgc tttagaagcc
    56641 gaaacctggg ctttcgctga ttccagcgca gctcgggcgc tctcagcggc ggcgcggtcg
    56701 tcatccagct gttgcgcaga aatagcccct cgttgggcga gcgagcggga acgcgtatga
    56761 cgtttggcaa cagagtccag ttcggcctgg cgttgattga ccagcgactg tgcggcacga
    56821 gtttcacttt gtcgttgctc cagcaaagcc tgcgcggcgg caacggcgct ttgtgcctct
    56881 ttgatttgcg cgatggcttc cagtcgctgt tcctgcaaca cgcgagtatc catcttcgcc
    56941 agcacttcgc cttcgcgaac aaactggcct tctttcacca gaatggtgtc gatacgcccg
    57001 gcaattttgc tggcgatatc cacttccgtc gcttcaatgc gcccattgct gacagcaaag
    57061 ccttccggca cacccgccgg gcgcaacagc caccacgcca cgacggcagc caccgccagt
    57121 gccccgacaa cccaccacgc cagatggcgc ttactcttat ccataatcga cccgccataa
    57181 tccctgtaag caaacgacgc gtgttacaac tgccgccgaa cgacgttcag cggattcttc
    57241 catccgggat aagaaggaaa cgacaaatac gaccagcgcg cagagcattg agcgccaacg
    57301 gggaaggctt agtgaaaacg ggtagtaatg ttgtcatcat cagcattcct ggccgtaaat
    57361 gaaacacgca tactcataag tgtgatctat aaaactggct gacaaaacgg tatccatcct
    57421 gttggatcgc atttcacgcc tgttcagttg caggaacaga gcattccttt ttacgtttta
    57481 tcaacagtct caaccagata gtaacattaa tattcatgtt gtctatggtt cagagctgta
    57541 acattgtgcg ccttctaatc aaaggcataa gtccatttct gtttttttca tgctggtaag
    57601 acttccaggg agtggttatc tctggccctg caccagggtt aatttggcaa ttatgcatgc
    57661 tgaacattct tccagcatgt ttactgtcac gggcgttaag acctaaaact gtccccgttt
    57721 cacctatgcg ttctctccca ccatttccgt tatgtgcaac aacttgattt atacctaaca
    57781 tttgtgaaca tgctaatagt gcttcaagtt taggtctgta atctgtcgaa acagaaaaat
    57841 aacgattaaa ggatcgacaa gagatagccc ggctagtttt aaactttttg ccattaatta
    57901 tagcatgatg ctttttatta ataatttccg ctaattcgac cggatcgaga gcattggtca
    57961 tttcatcaat agagacagtt atcggtccta taatgtaacg ttttcgctga tcatcaaaag
    58021 ttatcccatg atgatttgcc attattttgt atttggggtc atagaaacat acaggataat
    58081 cttttatccc gtcgtaggta tcctcatcaa gtggtttcat gcgcgtgaag ttttgcatat
    58141 agttaccatt gcaattcgtt tcatgatttc cagctaatac atgaacattt tcattgatac
    58201 tcattatgcg ttttaacaaa tcgatgataa attgatcgcc acagatatta ctaaatcgat
    58261 cgccggtttg gtcgccaaga aacgtacatg gcgtgactgc gtctttatta ataaccaaat
    58321 aaggctgaag actttctaaa acattatctc ttctgttcag tatactgatt attgtattaa
    58381 cattagtgtc aagttgctca ttaaagttct cccttagcaa tacgttgaga acttcatctt
    58441 ctatattaag caactggcta aggaaatttt ctccttcagc catgattccc aaatgtcctg
    58501 ttgcaaaaag cgcgtacaaa actgcaccga ctgagccatc ggtatcgcca aaataggtcg
    58561 gcctggtact tgtgggatca acaggtgaac aattggagaa ataggttctg tcagcgaact
    58621 ggggttttat aattgtttcg aatttaatat caaatacatt gtattgcgat atttcccagt
    58681 tccagatggg tttgtacgac tcagtaactg caacatcatt agcattatca tttactgtca
    58741 tattattaag taaataaaca atgctgcacc aattaatatc ggtattatca agagggcaac
    58801 cgtaattttt actattaatt gtcacaataa actgataaca gttattccta cgtagaatat
    58861 gagcgcgcat gcccgatagc atactatatt tttctgaatg attcgtttgt atggtaaatg
    58921 aaattttgtc atcttgaata gattgttgga aacgttctat ttgccaatca tcaatttttt
    58981 ccacaacttc accgagcatt gtcctggcaa tacttgatat atcatcacct ataccatttg
    59041 caatataccc aaacagttct ttagtgaagt tggtaacatg ctcttgcacc atatcatttg
    59101 ttgcgattgt cgttgtactg ccattagtgc ctgccacagt tccaattttc atcttttacc
    59161 tcatattatt caccgcgagg aatacatagt taacatttta tcttatgaca tacacctcat
    59221 attcgatggg gaaataatta tattttgcac aaatcattca gccttaaagc ataatatgaa
    59281 atttcttatc tcatagcaga gaaagtccct aaatattgta gaggcgtcat cagtacatac
    59341 ctctacaatc gattaagaaa acctatgaaa tactggcaaa attaaaaaga gataccacag
    59401 cactcatgaa gttttattga ggttgaaaac aactaagccc tgggacacct tttttatcac
    59461 cacgttcgtt aggtgctatc ttccaagaat ttagccccat ggtataatta tcaatttttt
    59521 gatgctggtc tacccctaat ccataatggc caaatttctg tttagttttt ttgagcgtct
    59581 gtccacattt gaagatatct tcaggttttg gtctgaaagc agtagtgtta ttaaaataat
    59641 tttcatatac aggtgtgggt gctatagcat tgcttctaat tagatggaga agatgatcat
    59701 ccatgtgttg tttatgtttt ttattacata tatttgctaa ttcctcagga tcaattgggt
    59761 ctttcatctg attaaatgga acctgcaaag caccgagaca ataacattga ttttcttcat
    59821 ttcttattat tccgtgatga ctggttaaaa ctttagtttc tgagttataa ttacaaacat
    59881 cgagcgtttt aattaagtta tacgtatcac cggcagagag tttataattt tctagtcgtg
    59941 cagtatagtt accattaaaa ttgatttcat gatttccagc cagaacgatg acatttttat
    60001 taatacggct atctttatta ccctccatat ttcgcataga atttagcaaa gtaaggatat
    60061 atttatcacc aaaaatagta ctaaatctat cgcctgtatg atccccaagg aagatacaag
    60121 gggttatcgc atcgtccttt aaaaaaacat gttgatgcaa ctcattcaat atcatgtctc
    60181 tcgtatttaa taaattgtag agcaatttat tatttttatg tatgtatgca gatgaagcca
    60241 tgtcttcgtg ttttaataat tcacataata aactccaacc cttttccctg atgcctatat
    60301 gccctgaaac gagcaatgaa taaagtacag cgcctactct tccatctgta tcgccaaaat
    60361 aagccgtgtt ggcattttgt gggttaatca caccacattg tttataaaag tctattttag
    60421 aaaacacaga tttaacatag ctatcaaact tatccttagg aaacgtctct ttagagacat
    60481 cccaacgcca gtcagcttta aaattaccgc taagaagagt aaagaaattt tcaggtatga
    60541 tagaaagccc attaacttta taaattatgt ctgatttatc tccggagaaa ttaataaaat
    60601 aataaccatc cttacactca atcttaactg taatgggaga cgataatttt atcacatggt
    60661 tattgtctgg tataattgta aagataaacg tgttagtagt attatcggca aaactattat
    60721 attcttgaat atctatagat ggctcactat tgtaaaacca tattttctcc atctgaagac
    60781 tatgttcatt taccctccag tgaccatgtt gcaggaaata gatgatatgc atgccaaaat
    60841 cgttatctat ttgcgaatca ttatccagct tatcaatttc aactatcttt gaacgatgtg
    60901 ttttatcaac cttgctatgg tgttgatgat tattaacttt atctgaatgc ttatctttat
    60961 ggtttgaagg gatgtactta aatatattgt caattgcttt cataaataac ccgcgatatt
    61021 tttgacctgt tagtaaaaaa taaatgatac atgtctgaaa gacatattta cgacgatata
    61081 aaacaataat acttatatgt tcgctgattt taagcaccga gactatccgg tcttttacta
    61141 aataatggaa aacaatccat ccctggaaga caattgtaaa aaaaacaaca gaataagaat
    61201 taatcttagg ataaatattt atttatcatg gccttttgac cgctaaaaaa tggcactcgg
    61261 cacagtaaac cccgctatag ttatgctatg catttgtctt taatgatatc tcgtcgttat
    61321 attaacaggc tgaaattatc attaatgcac atttcaatat tagcagggat acctgaggag
    61381 tttaaagtga acatatatat cgggtagctt ttcaaattaa tccctttgat tataggctta
    61441 atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaacgtca cgggtcatgt
    61501 gctgatttct ctggcggcca tatgcctggc attattcact accgcattta ttatcatttc
    61561 gcaactcacg cgcggcgtta atacgtttta caatacattg ttccccatta ttggctatgc
    61621 ggggtcaatt atcaccatga tatggggttg ggcactgtta gcaggtaatg atgtgatggc
    61681 agacgagttt gttgccggcc atgttatttt tggcgttggt atgattgccg catgtgtatc
    61741 gacggtggca gcgtcgtccg gtcactttct gctcattccc aaaaatgcag cggggagcaa
    61801 gagcgacgga acaccggtac aggcttattc ttcattaatc ggtaattgcc tcattgccgt
    61861 tcccgtttta ctcaccctgc tcggtttcat ttggtctatt acgctgttac atagtgccga
    61921 cataactccg cattatgtcg ccttgggtta accgcaatct gtgcctgtct aattggccta
    61981 gtggccacaa tcgtccatca aacacgtaat acgttctcat ccaaagagca ctggctgtgg
    62041 tgttattggg ttatttttct cggctcagtc acggtgctgc aggggatata cgtcttagtc
    62101 agttccgatg caagcgcccg actggctccc ggcattattc tcatttgcct cggaatgatc
    62161 tgttacagca tattctcaaa agtctggcta ctggcactgg tctggagacg tacctgttcg
    62221 ttagccaaca gaataccgat gataccggta tttacctgtc tgttttgctt gtttttgtca
    62281 tcattcctcg cggaaatcgc acaaactgag ctggactact ttattccatc tcgggttctg
    62341 gttggattag gcgcagtatg ttttacattg ttctctattg tttcaatatt agaggccggt
    62401 tctgcgaaaa aaaattaaaa tttacataat cccagcgtta ttcatatctt aacgctggtt
    62461 ttttacgaac ttagtgaaat aatgtagaat ataaaattat aattttatat tggagcacat
    62521 gtgttaatga tcaaccccgg gatactaaaa atatagacat tattaaactt gcttagcctc
    62581 gcattgcctt tacctgttaa agatgcatcg agaaaattga gtctgaagtt ctcatccttt
    62641 atctgatttt ccagtcccga aactttggcc ttagcattac ctgagatccg tccgttacta
    62701 tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc tcggtgtatc ttcatcagtt
    62761 aagccaacaa tcgtaaaact accgctacca tcttttttta aaatgactga aaggttagca
    62821 gagtagtata aagaatgatc tgttttagca tttcttgtta acgtcgacgt acattcaaga
    62881 gacgttgtgc taccatcacg agccttccag agagcaaaaa aacacacctg tagcaatgtg
    62941 aaaacaagta ttaatataaa atacattttc ttattagtca tataatagca cctctgaaca
    63001 aattgcataa gtttcgtcat tagaggattt tatacatctt gataaatacc gctgcccttc
    63061 cccaagcaaa taatgcatac tatttacatc atatataaaa tttgcacctg gcacacaggg
    63121 taaatgcttt ttcgaaattt cagcggcctc tatttgagat ttagcgatgc caatctcgtt
    63181 aagattctct gaaagatata aatcacaggt gtcaatctta cctgatttaa ccaatggata
    63241 atccgttata ctgtaggtat ctttaaaagc cacggcaaac atttcaataa taacaattac
    63301 taccacaagc gaaatcaaaa tgcgaaaaag aggaatactc cttttgcgct ctactttttc
    63361 aacaacatct tcttgtaaat gagaatcatc ataagattgt ttacttattg ttatgttttc
    63421 actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa caccaatatt
    63481 tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg acagatcaaa
    63541 tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc caccattact
    63601 ttccagtaac actgccaaaa tacaccccgc cgtagctgga aaatttatct catcctgggt
    63661 atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat tgataatgaa
    63721 aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat tttagcctat
    63781 tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa agtcatataa
    63841 aacttatatc cacagaataa aaaatcaaac atgtcagttt tatcaatata aagtatcgca
    63901 aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca tgaaattatc
    63961 aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta cttaagatga
    64021 ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat gactggtcag
    64081 ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag tcttttctgc
    64141 cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc tcataaaata
    64201 attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta aggctaaatc
    64261 actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt atgaccgggt
    64321 gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat ataatgaaaa
    64381 cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt tctctggcat
    64441 cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa atagaaaacg
    64501 ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca gatatactct
    64561 ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt aatgagattt
    64621 cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt gctatcaata
    64681 agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta attcagttca
    64741 actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt ttttataagt
    64801 taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc attattctgc
    64861 gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct gttctttcat
    64921 atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca tataacgctt
    64981 accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg tcagggaaac
    65041 tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg aagtactgct
    65101 ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc catcagatgc
    65161 gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat ataccccttt
    65221 cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat tagctgacgc
    65281 ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc ccgccagcat
    65341 gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta cactgcaaaa
    65401 tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca aataatggaa
    65461 acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac atgtcgccgc
    65521 tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg cgatacagga
    65581 ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca agccagaaac
    65641 ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt tgctataact
    65701 aatcacagcg ccgaagggga tgggtttacc ctctttctgt tttaaggtta ctaatccttt
    65761 atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg gtacaaccaa
    65821 ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca gaataacatc
    65881 gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag tactcagtcc
    65941 cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg ctaatgtccc
    66001 atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac cgcttaaatt
    66061 tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact gaccatatga
    66121 tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact gatgagcgtt
    66181 agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg ataatgaaac
    66241 ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat aaatttgatt
    66301 ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat ctactttcca
    66361 tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta tgcctctcca
    66421 taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg aagaattata
    66481 cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata aggatatcgt
    66541 agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg cacttactgc
    66601 ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DADLEE010000009        66032 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000009 DADLEE010000000
VERSION     DADLEE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.009"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH51_000620"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JQH51_000620"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY6075081.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH51_000621"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQH51_000621"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY6075082.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH51_000622"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQH51_000622"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY6075083.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /locus_tag="JQH51_000623"
     CDS             complement(3024..3479)
                     /locus_tag="JQH51_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY6075084.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH51_000624"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQH51_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY6075085.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH51_000625"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQH51_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY6075086.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH51_000626"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JQH51_000626"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY6075087.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="JQH51_000627"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="JQH51_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY6075088.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="JQH51_000628"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="JQH51_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY6075089.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="JQH51_000629"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="JQH51_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY6075090.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="JQH51_000630"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="JQH51_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY6075091.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="JQH51_000631"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="JQH51_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY6075092.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQH51_000632"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQH51_000632"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY6075093.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="JQH51_000633"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="JQH51_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY6075094.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="JQH51_000634"
     CDS             19304..19369
                     /locus_tag="JQH51_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075095.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="JQH51_000635"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="JQH51_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY6075096.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="JQH51_000636"
     CDS             complement(19753..20376)
                     /locus_tag="JQH51_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY6075097.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="JQH51_000637"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="JQH51_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY6075098.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="JQH51_000638"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="JQH51_000638"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY6075099.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="JQH51_000639"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="JQH51_000639"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY6075100.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="JQH51_000640"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="JQH51_000640"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY6075101.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /locus_tag="JQH51_000641"
                     /pseudo
     CDS             25215..26371
                     /locus_tag="JQH51_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26460..26846)
                     /locus_tag="JQH51_000642"
     CDS             complement(26460..26846)
                     /locus_tag="JQH51_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY6075102.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="JQH51_000643"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="JQH51_000643"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY6075103.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="JQH51_000644"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="JQH51_000644"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY6075104.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="JQH51_000645"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="JQH51_000645"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY6075105.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="JQH51_000646"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="JQH51_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY6075106.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="JQH51_000647"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="JQH51_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY6075107.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="JQH51_000648"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="JQH51_000648"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY6075108.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="JQH51_000649"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="JQH51_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY6075109.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="JQH51_000650"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="JQH51_000650"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY6075110.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="JQH51_000651"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="JQH51_000651"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY6075111.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="JQH51_000652"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="JQH51_000652"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY6075112.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="JQH51_000653"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="JQH51_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY6075113.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="JQH51_000654"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="JQH51_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY6075114.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="JQH51_000655"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="JQH51_000655"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY6075115.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="JQH51_000656"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="JQH51_000656"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY6075116.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="JQH51_000657"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="JQH51_000657"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY6075117.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="JQH51_000658"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="JQH51_000658"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY6075118.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="JQH51_000659"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="JQH51_000659"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY6075119.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="JQH51_000660"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="JQH51_000660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY6075120.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="JQH51_000661"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="JQH51_000661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY6075121.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="JQH51_000662"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="JQH51_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY6075122.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="JQH51_000663"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="JQH51_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY6075123.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="JQH51_000664"
     CDS             53914..54303
                     /locus_tag="JQH51_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6075124.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="JQH51_000665"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="JQH51_000665"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY6075125.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="JQH51_000666"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="JQH51_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY6075126.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="JQH51_000667"
     CDS             complement(55962..56162)
                     /locus_tag="JQH51_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY6075127.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="JQH51_000668"
     CDS             56328..56873
                     /locus_tag="JQH51_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY6075128.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="JQH51_000669"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="JQH51_000669"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY6075129.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="JQH51_000670"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="JQH51_000670"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY6075130.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="JQH51_000671"
     CDS             complement(58171..58995)
                     /locus_tag="JQH51_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY6075131.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQH51_000672"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQH51_000672"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY6075132.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQH51_000673"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQH51_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY6075133.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQH51_000674"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQH51_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY6075134.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQH51_000675"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQH51_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY6075135.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQH51_000676"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQH51_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY6075136.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQH51_000677"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQH51_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY6075137.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="JQH51_000678"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="JQH51_000678"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY6075138.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="JQH51_000679"
     rRNA            65762..>66032
                     /locus_tag="JQH51_000679"
                     /product="16S ribosomal RNA"
BASE COUNT        15626 a        16346 c        18197 g        15863 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cttgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DADLEE010000010        60395 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000010 DADLEE010000000
VERSION     DADLEE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60395
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.010"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..322
                     /locus_tag="JQH51_000680"
     CDS             <1..322
                     /locus_tag="JQH51_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6075139.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="JQH51_000681"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="JQH51_000681"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY6075140.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="JQH51_000682"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="JQH51_000682"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY6075141.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /locus_tag="JQH51_000683"
     CDS             complement(2166..3068)
                     /locus_tag="JQH51_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY6075142.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="JQH51_000684"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="JQH51_000684"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY6075143.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="JQH51_000685"
     CDS             complement(3726..4955)
                     /locus_tag="JQH51_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY6075144.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="JQH51_000686"
     CDS             complement(5019..5459)
                     /locus_tag="JQH51_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY6075145.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="JQH51_000687"
     CDS             complement(5564..6334)
                     /locus_tag="JQH51_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6075146.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="JQH51_000688"
     CDS             complement(6331..7257)
                     /locus_tag="JQH51_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6075147.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="JQH51_000689"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="JQH51_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY6075148.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="JQH51_000690"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="JQH51_000690"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY6075149.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /locus_tag="JQH51_000691"
     CDS             8811..11201
                     /locus_tag="JQH51_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY6075150.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="JQH51_000692"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="JQH51_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY6075151.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="JQH51_000693"
     CDS             12964..13311
                     /locus_tag="JQH51_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY6075152.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="JQH51_000694"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="JQH51_000694"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY6075153.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="JQH51_000695"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="JQH51_000695"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY6075154.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="JQH51_000696"
     CDS             complement(15131..15493)
                     /locus_tag="JQH51_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY6075155.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="JQH51_000697"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="JQH51_000697"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY6075156.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="JQH51_000698"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="JQH51_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="JQH51_000699"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="JQH51_000699"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY6075157.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24149)
                     /gene="aceF"
                     /locus_tag="JQH51_000700"
     CDS             complement(22293..24149)
                     /gene="aceF"
                     /locus_tag="JQH51_000700"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY6075158.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAK
                     VKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSKFGEIEEVE
                     LGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLDVKITPVVF
                     IMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKGII
                     ELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKS
                     AMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM"
     gene            complement(24164..26827)
                     /gene="aceE"
                     /locus_tag="JQH51_000701"
     CDS             complement(24164..26827)
                     /gene="aceE"
                     /locus_tag="JQH51_000701"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY6075159.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26988..27752)
                     /gene="pdhR"
                     /locus_tag="JQH51_000702"
     CDS             complement(26988..27752)
                     /gene="pdhR"
                     /locus_tag="JQH51_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY6075160.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28293..29666
                     /gene="aroP"
                     /locus_tag="JQH51_000703"
     CDS             28293..29666
                     /gene="aroP"
                     /locus_tag="JQH51_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY6075161.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29709..30563)
                     /gene="ampE"
                     /locus_tag="JQH51_000704"
     CDS             complement(29709..30563)
                     /gene="ampE"
                     /locus_tag="JQH51_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY6075162.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30560..31111)
                     /gene="ampD"
                     /locus_tag="JQH51_000705"
     CDS             complement(30560..31111)
                     /gene="ampD"
                     /locus_tag="JQH51_000705"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY6075163.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31199..32092
                     /gene="nadC"
                     /locus_tag="JQH51_000706"
     CDS             31199..32092
                     /gene="nadC"
                     /locus_tag="JQH51_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY6075164.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32295..32735
                     /gene="ppdD"
                     /locus_tag="JQH51_000707"
     CDS             32295..32735
                     /gene="ppdD"
                     /locus_tag="JQH51_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY6075165.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32745..33892
                     /gene="cpaF"
                     /locus_tag="JQH51_000708"
                     /pseudo
     CDS             32745..33892
                     /gene="cpaF"
                     /locus_tag="JQH51_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
     gene            33882..35084
                     /gene="hofC"
                     /locus_tag="JQH51_000709"
     CDS             33882..35084
                     /gene="hofC"
                     /locus_tag="JQH51_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY6075166.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35119..36162)
                     /gene="guaC"
                     /locus_tag="JQH51_000710"
     CDS             complement(35119..36162)
                     /gene="guaC"
                     /locus_tag="JQH51_000710"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY6075167.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36387..37007
                     /gene="coaE"
                     /locus_tag="JQH51_000711"
     CDS             36387..37007
                     /gene="coaE"
                     /locus_tag="JQH51_000711"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY6075168.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37007..37750
                     /gene="zapD"
                     /locus_tag="JQH51_000712"
     CDS             37007..37750
                     /gene="zapD"
                     /locus_tag="JQH51_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY6075169.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37760..37957
                     /gene="yacG"
                     /locus_tag="JQH51_000713"
     CDS             37760..37957
                     /gene="yacG"
                     /locus_tag="JQH51_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY6075170.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38057..38446)
                     /gene="mutT"
                     /locus_tag="JQH51_000714"
     CDS             complement(38057..38446)
                     /gene="mutT"
                     /locus_tag="JQH51_000714"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY6075171.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38506..41211)
                     /gene="secA"
                     /locus_tag="JQH51_000715"
     CDS             complement(38506..41211)
                     /gene="secA"
                     /locus_tag="JQH51_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY6075172.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41273..41785)
                     /gene="secM"
                     /locus_tag="JQH51_000716"
     CDS             complement(41273..41785)
                     /gene="secM"
                     /locus_tag="JQH51_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY6075173.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42016..42933)
                     /gene="lpxC"
                     /locus_tag="JQH51_000717"
     CDS             complement(42016..42933)
                     /gene="lpxC"
                     /locus_tag="JQH51_000717"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY6075174.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43034..44185)
                     /gene="ftsZ"
                     /locus_tag="JQH51_000718"
     CDS             complement(43034..44185)
                     /gene="ftsZ"
                     /locus_tag="JQH51_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY6075175.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44246..45508)
                     /gene="ftsA"
                     /locus_tag="JQH51_000719"
     CDS             complement(44246..45508)
                     /gene="ftsA"
                     /locus_tag="JQH51_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY6075176.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45505..46335)
                     /gene="ftsQ"
                     /locus_tag="JQH51_000720"
     CDS             complement(45505..46335)
                     /gene="ftsQ"
                     /locus_tag="JQH51_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY6075177.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46337..47257)
                     /locus_tag="JQH51_000721"
     CDS             complement(46337..47257)
                     /locus_tag="JQH51_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY6075178.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47250..48725)
                     /gene="murC"
                     /locus_tag="JQH51_000722"
     CDS             complement(47250..48725)
                     /gene="murC"
                     /locus_tag="JQH51_000722"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY6075179.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48779..49846)
                     /gene="murG"
                     /locus_tag="JQH51_000723"
     CDS             complement(48779..49846)
                     /gene="murG"
                     /locus_tag="JQH51_000723"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY6075180.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49843..51087)
                     /gene="ftsW"
                     /locus_tag="JQH51_000724"
     CDS             complement(49843..51087)
                     /gene="ftsW"
                     /locus_tag="JQH51_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY6075181.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51087..52403)
                     /gene="murD"
                     /locus_tag="JQH51_000725"
     CDS             complement(51087..52403)
                     /gene="murD"
                     /locus_tag="JQH51_000725"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY6075182.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52406..53488)
                     /gene="mraY"
                     /locus_tag="JQH51_000726"
     CDS             complement(52406..53488)
                     /gene="mraY"
                     /locus_tag="JQH51_000726"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY6075183.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53482..54840)
                     /gene="murF"
                     /locus_tag="JQH51_000727"
     CDS             complement(53482..54840)
                     /gene="murF"
                     /locus_tag="JQH51_000727"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY6075184.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54837..56324)
                     /gene="murE"
                     /locus_tag="JQH51_000728"
     CDS             complement(54837..56324)
                     /gene="murE"
                     /locus_tag="JQH51_000728"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY6075185.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56311..58077)
                     /locus_tag="JQH51_000729"
     CDS             complement(56311..58077)
                     /locus_tag="JQH51_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY6075186.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58093..58458)
                     /gene="ftsL"
                     /locus_tag="JQH51_000730"
     CDS             complement(58093..58458)
                     /gene="ftsL"
                     /locus_tag="JQH51_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY6075187.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58455..59396)
                     /gene="rsmH"
                     /locus_tag="JQH51_000731"
     CDS             complement(58455..59396)
                     /gene="rsmH"
                     /locus_tag="JQH51_000731"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY6075188.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59398..59856)
                     /gene="mraZ"
                     /locus_tag="JQH51_000732"
     CDS             complement(59398..59856)
                     /gene="mraZ"
                     /locus_tag="JQH51_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY6075189.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16724 c        14840 g        14358 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgctgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgagct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgaaccagtt
    23341 ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccgga
    23401 acttccatag aagctttatc gccttctacg gtaatcagtg actgttcagc ggcaactttg
    23461 tcgcccactt tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg
    23521 ttaacttctt tcacgccagc tgctggtgca ggggtcgctg ccggagtcgc ttcctgttta
    23581 gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg
    23641 gtagacactt tgtcacccac gttcactttg atctctttca cggtacctgc aaacggagcc
    23701 ggaacttcca tagaagcctt gtcgccttct acggtgatca gcgactgttc agcttcaact
    23761 ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga
    23821 acgttaacgt cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc ttctttcttc
    23881 tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc
    23941 agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac gatacccgcc
    24001 tgcggagatg gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg
    24061 gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg
    24121 atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt
    24181 taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga
    24241 tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt
    24301 cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta
    24361 cgcgatagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt
    24421 cggtagatgc cactgccggc gcgtcgttca tcacctgagc gatatacgga acgcgcggag
    24481 tttccagcgg atgcagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg
    24541 tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg
    24601 ccgcttcacg gacgtgacgc aggatggaac cggagcccag cagctgaact ttacctttgc
    24661 taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg
    24721 gcattgccgg catgtggtag ttttcgttca gcgtagtgat gtagtagtaa acgttctctt
    24781 gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact tcgtaagcgt
    24841 aagccgggtc gtaagagata cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat
    24901 cttcgtgctg cagaccttca ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga
    24961 agccacgcgc ttgctggtct ccagccgccc agcacagatc gccgatacgc tggaagccga
    25021 acatcgagta atagatgtag aatgggatca tcggcagatt gttcgtgctg taagaggtcg
    25081 ccgctgccag ccaggaacac cctgcgccca gctcgttgat cccttcctgc agaatctgac
    25141 ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac
    25201 cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt
    25261 cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc atcacgttca
    25321 gagcacgaac gaaagcgata gtggtagaga gctctttgct ctgctcttcc agcagcgcgc
    25381 cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca
    25441 gataaccgtg cagtttctga cgctgagcgt gcagataggt atgctcttca gaaccttccg
    25501 ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt
    25561 cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac
    25621 cttcagccgc gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt
    25681 tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac
    25741 cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag
    25801 gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt
    25861 agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca
    25921 gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga
    25981 agatgccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct
    26041 gcaggttaca gttgataacg aaaaccaggt tatccagttt ttcacgggta gcgatggtga
    26101 tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct
    26161 gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa
    26221 tagcaccaat cggacccaga cccatagata cggtcgggaa ctgccagaat tccggcatca
    26281 gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca
    26341 gctgctcctg agtcagacga ccttccagga aagcacgggc gtaaacgccc ggggagatgt
    26401 ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt
    26461 taaagcacac atcataaatg gttgcggaag actggaagga cgccatgtgg ccgcccagtt
    26521 cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc gttccagcgg atagctgaac
    26581 gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg
    26641 tgttgatgta gttgctgata cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt
    26701 cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg
    26761 attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt
    26821 ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt
    26881 gaactctcgc cagaaagctc aataagacag gttctacgtt tagttaccgc gcttttatat
    26941 gcgcttgatt tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt
    27001 tgctccagac gacgcagaga acgctcacgg cggctctctt cacggcttct gtcgagcaaa
    27061 atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg
    27121 accataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga
    27181 cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac
    27241 cttagcagat gaagcagaac cacattgtgg gccgcttcgg tgacggcaat ctgatactgg
    27301 agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagctc tatggcgtgg
    27361 tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg
    27421 gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgattc aggatggtcg
    27481 gagagcagct ccaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg
    27541 ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc
    27601 aaggagggac gggagacgtc gaactgtttt gccagttcgc gttccggtgg gagtttttcg
    27661 cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag
    27721 agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa
    27781 gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat
    27841 gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata
    27901 taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa gggatttttt
    27961 aacgattatt aaactataaa aatccgaatt gaaccgattc acttaccaat tttttgattt
    28021 ttaagtcaat aaaaacgaat ttaaattcac tctgcatatt gagaggggtt gaggctgagc
    28081 tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc
    28141 gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacctcc
    28201 ttgatgtaaa caaatcaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
    28261 agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
    28321 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
    28381 ggacagggct attcctaggt agcgcctccg taatacagtc cgcagggcca gggattatcc
    28441 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
    28501 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
    28561 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggcag
    28621 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatccca acctgggttt
    28681 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
    28741 gcgagatgga gttctggttt gccattatca aagttattgc ggtagtagcg atgatcatct
    28801 tcggcggctg gctgctgttc agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt
    28861 gggatcaagg tggtttcctg ccgcacggct tcaccggact ggtgatgatg atggcgatta
    28921 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
    28981 cggagcaaag tatcccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata
    29041 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
    29101 gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca
    29161 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
    29221 tgctgtttgg tctggcacaa caggggaacg cgccaaaagc gctggcgtct gtcgataaac
    29281 gcggcgtacc ggttaacacc attctggtgt ctgcgctggt tacagcattg tgcgtattga
    29341 ttaactatct tgctccggaa tccgcatttg gcctgttaat ggcactggtg gtatccgcac
    29401 tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg
    29461 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
    29521 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
    29581 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
    29641 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc
    29701 aataatctct acaccaacgc cccgtaaatc gtcagtagcg caatcaccac cacgaccacg
    29761 aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt atcgacatgc
    29821 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaata cctgatattg cgaagtatgg
    29881 aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca
    29941 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac tgcatcaatg
    30001 ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc
    30061 aagaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac
    30121 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
    30181 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
    30241 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
    30301 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag cagcgtggga
    30361 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacacc catcgcaata
    30421 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
    30481 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
    30541 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga aaccgtgccc
    30601 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
    30661 tgttattagc gatatcggga tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt
    30721 gatactgcgc atcggtatac gccagagtat cggtgccttc aagctcaatc ccaatagaaa
    30781 aatcattgca gcgttcacgc ccctgatact gagagactcc cgcatgccag gcacgtttat
    30841 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
    30901 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
    30961 atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca
    31021 gcagggtggg tgtttcgtca tccaggcggc aatcgtaatg tggtgaggga acgcggcgcg
    31081 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
    31141 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
    31201 gccgcctcgc cgctacaacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
    31261 cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
    31321 caacaatgac attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat
    31381 cacccgcgag aatggcgtat tttgtggcaa acgctgggtt gaagaggtgt ttattcaact
    31441 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaacgccaa
    31501 tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
    31561 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
    31621 actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg gcctgcgttc
    31681 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
    31741 tgccttcctg atcaaagaaa accatattat tgtctccggc tcagtgcgcc aggcggtcga
    31801 aaaagcgtcc tggctgcatc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
    31861 acttgatgag gccctgaaag ccggagccga tatcatcatg ctggataact tcgaaacaga
    31921 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
    31981 cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg
    32041 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aattgctgac
    32101 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
    32161 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg
    32221 tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcac caaccaaatc
    32281 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
    32341 ttggcatcat tgccatttta agctccattg gtattcccgc ttatcaaaac tacctgcgca
    32401 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
    32461 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
    32521 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
    32581 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg
    32641 gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga cagcgcattg cagcaagcct
    32701 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
    32761 tcacggccct gtgcctgcgt tatcagggag tcttgctgga tgccagcgaa gaagtggttc
    32821 atgttgcggt ggtcgatgcc ccctcacatg agttgctgga cgcattgcat ttcgctacca
    32881 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
    32941 cacaacagac attacccgta gctgttcagg agaagcatca gcccaaagca gagttgctga
    33001 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
    33061 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
    33121 caccggatgc cggagtcgca ttaacagcca gattaaaagt gctgggaaac ctggatattg
    33181 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacaccgtct
    33241 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
    33301 agcaggtgag ccaggcactg gatgttaaca cgctgggaat gcagccgtta caactggcgg
    33361 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
    33421 gtggcaaaac ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct
    33481 ggtacgttta cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt
    33541 aatagcccag cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc
    33601 catccatatt ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg
    33661 tgtacattgc taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac
    33721 accagccata cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc
    33781 ccgacaggcg ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt
    33841 aaccaccttt gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct
    33901 ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga
    33961 gccgtacttt actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc
    34021 gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac
    34081 tggcgacgct acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac
    34141 agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg
    34201 gcattgcttt ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg
    34261 cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc
    34321 gtcagcaaaa atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cgttatccca
    34381 tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg
    34441 agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg caggggatca
    34501 tgacgctggc agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc
    34561 tggcgatagc caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat
    34621 tgctgttacg catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct
    34681 ttacgattct ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg
    34741 tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata
    34801 tcagtaacgg tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct
    34861 gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc
    34921 tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg
    34981 aaccggcgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc
    35041 tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc
    35101 cacgccagcg ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg
    35161 aataaacgtg gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc
    35221 tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca acctggcctc gcagcggcag
    35281 cttaacggtt ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat
    35341 cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat
    35401 gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc
    35461 gccgccaaag gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat
    35521 tccgcccaga ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc
    35581 gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat
    35641 atcagcaccg gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca
    35701 aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga
    35761 ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg
    35821 tttagttttt tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac
    35881 atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac
    35941 agcagtcaga atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc
    36001 catatttgcg gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa
    36061 ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag
    36121 aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt
    36181 aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg
    36241 gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat
    36301 agtgagcgaa cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc
    36361 atgatgaatt cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg
    36421 gcagtggtaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg
    36481 ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg
    36541 atcactttgg cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg
    36601 agcggatctt cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga
    36661 ttcaacaaga gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg
    36721 tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg
    36781 tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg
    36841 tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg
    36901 tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac
    36961 actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt
    37021 cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat
    37081 tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg
    37141 taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa
    37201 agaacttaac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca
    37261 gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gtgagcgtat taatttccgc
    37321 gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact
    37381 gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct
    37441 accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac
    37501 ccaggcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac
    37561 cagcctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct
    37621 gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg
    37681 ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc
    37741 ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa
    37801 aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct
    37861 gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc
    37921 cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag
    37981 gcctgataag acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt
    38041 gaacgcctta tccgacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg
    38101 gaaaatcatc ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac
    38161 cccacggccc cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg
    38221 ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt
    38281 cctgaagttc acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg
    38341 gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat
    38401 tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat
    38461 aaaaaaggcg cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc
    38521 atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt
    38581 gcgttcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct
    38641 aagctgctgc atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc
    38701 aacctcttca ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa
    38761 cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc
    38821 tttctgtgca tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg
    38881 ctctttccac agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat
    38941 ctcagcacca accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg
    39001 ctcacgcagc gtctcttcat gcagttctgg ttctttatcc agccactcgg taattggtaa
    39061 atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc
    39121 cagcgactgt ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct
    39181 gttgatggtt tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc
    39241 gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt
    39301 acgactttca actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat
    39361 ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa
    39421 aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg
    39481 acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc
    39541 gatgatatgc aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt
    39601 aattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct
    39661 acctccgagc acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc
    39721 cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac
    39781 attgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga
    39841 gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat
    39901 cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat
    39961 tggacggttg gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc
    40021 ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg
    40081 gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc
    40141 cacagcctgg tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc
    40201 gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc
    40261 atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag
    40321 agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc
    40381 acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg
    40441 gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg
    40501 tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc
    40561 atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg
    40621 ttcttcaggg ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt
    40681 accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag
    40741 gttgatacca acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg
    40801 ttgcgccagg tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag
    40861 gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca
    40921 gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac
    40981 gcgcttactt gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc
    41041 gcctttttcc agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag
    41101 tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag
    41161 ggtgcgatcg ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc
    41221 tcaaacgccc cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga
    41281 ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt
    41341 ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt
    41401 cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca
    41461 aagattcttc agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta
    41521 ccgtacgaat agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg
    41581 tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc
    41641 gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta
    41701 aactcgccgc gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc
    41761 gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta
    41821 gcgcacaaat cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg
    41881 taaaagtgct taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa
    41941 aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg
    42001 acagtataaa tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc
    42061 tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag
    42121 cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg
    42181 accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc
    42241 aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa
    42301 gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa
    42361 cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg
    42421 ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc
    42481 attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag tctctttgat
    42541 gcgaacaaat tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac
    42601 aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat
    42661 gttatcgatg cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac
    42721 atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc
    42781 cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg
    42841 cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg
    42901 aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc
    42961 aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa
    43021 ttccagtcaa ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg
    43081 gctctttcgc agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct
    43141 gggtcagcgg agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct
    43201 tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg
    43261 ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt
    43321 tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt
    43381 cgaagcccgc cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca
    43441 gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca
    43501 cgccagaacc catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt
    43561 ccacgttcat caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca
    43621 gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc agaactttca
    43681 gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc
    43741 cctgctcggc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag
    43801 caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac
    43861 cggtaccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac
    43921 gcaatgcatc gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc
    43981 ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca
    44041 gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca
    44101 tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat
    44161 tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgagtcc
    44221 gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct
    44281 tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat
    44341 gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat
    44401 aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc
    44461 gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg
    44521 ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt
    44581 cgttgaccag gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac
    44641 gttgcagact ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc
    44701 caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg
    44761 gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct
    44821 ttgtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat
    44881 cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca
    44941 gtccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt
    45001 ttttcgccat atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg
    45061 aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca
    45121 catgcagcac acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt
    45181 ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc
    45241 agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca
    45301 attctgcctg gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc
    45361 cgcctttatc cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt
    45421 cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta
    45481 ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg
    45541 agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa
    45601 atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag
    45661 ttctacaaag cgaaccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc
    45721 gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa
    45781 tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct
    45841 gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct
    45901 gaaggtgttt ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata
    45961 ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat
    46021 ccacggcagg cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt
    46081 acccggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg
    46141 ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa
    46201 cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc
    46261 cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag
    46321 agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc
    46381 tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg
    46441 tattggcttc cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc
    46501 atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt
    46561 gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct
    46621 cataatcata gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga
    46681 gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt
    46741 gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc
    46801 ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt
    46861 ctgctaactg cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg
    46921 gcgcgaccgg taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa
    46981 gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct
    47041 gcagcgtacc atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca
    47101 tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc
    47161 cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg
    47221 aagtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc
    47281 ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga
    47341 acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg
    47401 tccggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg
    47461 ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta
    47521 tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg
    47581 tgcggctgaa acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg
    47641 gtggcgtcaa cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt
    47701 ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc
    47761 tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc
    47821 gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcac
    47881 atcggctctt tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa
    47941 tcttctacac gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca
    48001 cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa
    48061 ggcaggttgt gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag
    48121 gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg
    48181 aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca
    48241 tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct
    48301 gcgtagatgc tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg
    48361 atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata
    48421 cgcgcttcat gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg
    48481 accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga
    48541 ttcagtaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct
    48601 tcattggcca gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt
    48661 atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta
    48721 ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt
    48781 acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg
    48841 cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt
    48901 tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt
    48961 ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg
    49021 ccggtagtcc tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga
    49081 catccgccca cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg
    49141 gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat
    49201 gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa
    49261 tgcgtgcgcc ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca
    49321 aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt
    49381 ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc
    49441 atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg
    49501 accacgcggc cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag
    49561 gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg
    49621 cagctatcag tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt
    49681 cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa
    49741 cttgccaacc ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac
    49801 cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac
    49861 aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc
    49921 tgtcgacata atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt
    49981 ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat
    50041 gccaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc
    50101 aatcgacatc gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac
    50161 ataacctaat tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata
    50221 ctccagtttt tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc
    50281 catcagcgat tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa
    50341 tgcggtaaca cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat
    50401 gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc
    50461 cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac
    50521 tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc
    50581 gtcgccttta cgcaccagat agttggcgat atagcaaaac agcgacagtt ttgtcagctc
    50641 cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct
    50701 acccactacc agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta
    50761 gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat
    50821 cagatagaca ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat
    50881 gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca
    50941 cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac
    51001 ccagcccttt agcgccgtgg agatccagac caggatactg aatcctggca ggcgcggcat
    51061 tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca
    51121 ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga
    51181 accatatcgc ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg
    51241 gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa
    51301 tacagacgta cgttatcgcc attcaggtaa cgcaccagtg ggctaaagtc cgccgattta
    51361 ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct
    51421 tccgtactgc cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc
    51481 agcaccactt caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg
    51541 gcgcgcggta accctgcggc atctgccagc gccagcgcca ccagcgcatt ggtgtagtta
    51601 tgctgcccgg aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc
    51661 caggtttcgc cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg
    51721 cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa
    51781 actttcgcgt tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg
    51841 tagcgatcca tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg
    51901 gaggtggttt ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc
    51961 agtagcatca acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct
    52021 ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc
    52081 acaatcggtg cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg
    52141 gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc
    52201 agatctgccg ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg
    52261 ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg
    52321 cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca
    52381 tttttaccct ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga
    52441 ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca
    52501 gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg
    52561 agccgacctg caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca
    52621 gcaggaattc ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg
    52681 aaccgacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc
    52741 ctgccccgac tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta
    52801 tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac
    52861 cggcgacaaa tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg
    52921 cgttgccagt acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg
    52981 gcatcacatc tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa
    53041 ggtacaaagc gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag
    53101 cgatcaaccc tttggtgtct ttacgcacca ctttgcgata atcatcaaca aagccaataa
    53161 caccgtaacc taccagcacc accaacacgc accagacgta cggattggac gggtaagccc
    53221 acagcagcac ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac
    53281 cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt
    53341 tttgcaaatg agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca
    53401 ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga
    53461 ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc
    53521 ttccatggca gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat
    53581 cagtgattta agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct
    53641 ggcggtgctg atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc
    53701 ttttgccgcc tcacccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat
    53761 atcgcccacc accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt
    53821 cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg
    53881 gatggggaac agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc
    53941 aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg
    54001 acgccccggc aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc
    54061 cataccgtgc gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg
    54121 tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt
    54181 gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc
    54241 tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac
    54301 cagcgccgct tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc
    54361 gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac
    54421 accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat
    54481 cgccgccgtc atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgag
    54541 cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt
    54601 gacgattaac tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc
    54661 gccagctttc gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac
    54721 aaacaggcag cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc
    54781 tgcaccttgc agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat
    54841 gcaatccccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg
    54901 ccaacaatct ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta
    54961 gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg
    55021 gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg
    55081 ttatcgtccg tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga
    55141 cgcttacctt tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag
    55201 tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc
    55261 accaccgtcg gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga
    55321 cgcgcggcgg ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc
    55381 gcgagcaaca ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg
    55441 ccccagcttg aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct
    55501 ttcaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg
    55561 tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga
    55621 ccgcaatgat gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca
    55681 tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc
    55741 gccacacggt gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc
    55801 tgatccacca gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct
    55861 gtcgggatca ctttccccag cagaccgtta ccaacggtgc ccattaccgc gctggtttcg
    55921 ccaagcagtt ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc
    55981 gttacgccta cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca
    56041 gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt
    56101 tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc
    56161 gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg
    56221 cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct
    56281 ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg
    56341 attaatcaca aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt
    56401 acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta
    56461 tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg
    56521 cgcaacgcct gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt
    56581 tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc
    56641 gccgcctggt agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg
    56701 gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat
    56761 gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc
    56821 gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa
    56881 gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta
    56941 agtatctact aacactgagg acggcatcgt taacgccagc ttggaaacac cgacgttact
    57001 cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc
    57061 gtggccgtta attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg
    57121 ttgcaacgcg gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt
    57181 gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga
    57241 cgggctgtta gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc
    57301 agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag
    57361 gcgttcatca atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc
    57421 ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag
    57481 ccatttatcg aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc
    57541 gatgaggtga gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg
    57601 aatccccggc agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc
    57661 cagataaata aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg
    57721 aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc
    57781 atgcacttct ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg
    57841 accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg
    57901 catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc
    57961 gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat
    58021 aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg
    58081 tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat
    58141 ttgcagcttt tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc
    58201 aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg
    58261 ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat
    58321 aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac
    58381 accaggcaat gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc
    58441 tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca
    58501 cgagggttct cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga
    58561 cggccaccca gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga
    58621 ccgcggctgt tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg
    58681 atgatcgaaa gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct
    58741 atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga
    58801 tgtttaaact tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg
    58861 cgggtcatcg gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa
    58921 tgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc
    58981 cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc
    59041 ataaaggaaa agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg
    59101 agaatgccgt cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag
    59161 aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc
    59221 tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga
    59281 cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg
    59341 aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta
    59401 tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat
    59461 atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg
    59521 tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa
    59581 caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac
    59641 acggcgctca accgggttca tgctcaacag acgcgataat ttttgctcga taatttccca
    59701 ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg
    59761 accggcagcg ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt
    59821 gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac
    59881 tttgccccac aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc
    59941 agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta
    60001 ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc
    60061 atcgtcatca ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt
    60121 tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg
    60181 tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc
    60241 aattcatcgc cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat
    60301 tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag
    60361 tcaggaatag tcttatttac tttaaggata ttgat
//
LOCUS       DADLEE010000011        58573 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000011 DADLEE010000000
VERSION     DADLEE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.011"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1100)
                     /locus_tag="JQH51_000733"
     CDS             complement(<1..1100)
                     /locus_tag="JQH51_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY6075190.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JQH51_000734"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JQH51_000734"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY6075191.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JQH51_000735"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JQH51_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY6075192.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JQH51_000736"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JQH51_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY6075193.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JQH51_000737"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JQH51_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY6075194.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JQH51_000738"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JQH51_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY6075195.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="JQH51_000739"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="JQH51_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY6075196.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JQH51_000740"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JQH51_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY6075197.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /locus_tag="JQH51_000741"
     CDS             complement(5804..7084)
                     /locus_tag="JQH51_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY6075198.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="JQH51_000742"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="JQH51_000742"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY6075199.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="JQH51_000743"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="JQH51_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY6075200.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="JQH51_000744"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="JQH51_000744"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY6075201.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /locus_tag="JQH51_000745"
     CDS             10283..11701
                     /locus_tag="JQH51_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY6075202.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="JQH51_000746"
     CDS             11850..12014
                     /locus_tag="JQH51_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075203.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="JQH51_000747"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="JQH51_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY6075204.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="JQH51_000748"
     CDS             13523..13768
                     /locus_tag="JQH51_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY6075205.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="JQH51_000749"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="JQH51_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY6075206.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /locus_tag="JQH51_000750"
     CDS             15721..16686
                     /locus_tag="JQH51_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial peptide ABC transporter permease
                     SapB"
                     /protein_id="HAY6075207.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="JQH51_000751"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="JQH51_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY6075208.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="JQH51_000752"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="JQH51_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY6075209.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="JQH51_000753"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="JQH51_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY6075210.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="JQH51_000754"
     CDS             19431..19784
                     /locus_tag="JQH51_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY6075211.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="JQH51_000755"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="JQH51_000755"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY6075212.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="JQH51_000756"
     CDS             21084..22211
                     /locus_tag="JQH51_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY6075213.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="JQH51_000757"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="JQH51_000757"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY6075214.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="JQH51_000758"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="JQH51_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..24909
                     /locus_tag="JQH51_000759"
     CDS             24448..24909
                     /locus_tag="JQH51_000759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075215.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDALRFSTTKTTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIG"
     gene            24928..25629
                     /locus_tag="JQH51_000760"
                     /pseudo
     CDS             24928..25629
                     /locus_tag="JQH51_000760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            25642..26422
                     /locus_tag="JQH51_000761"
                     /pseudo
     CDS             25642..26422
                     /locus_tag="JQH51_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            26570..26743
                     /locus_tag="JQH51_000762"
     CDS             26570..26743
                     /locus_tag="JQH51_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075216.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="JQH51_000763"
     CDS             26833..27582
                     /locus_tag="JQH51_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6075217.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="JQH51_000764"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="JQH51_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY6075218.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="JQH51_000765"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="JQH51_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY6075219.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="JQH51_000766"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="JQH51_000766"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY6075220.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="JQH51_000767"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="JQH51_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY6075221.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="JQH51_000768"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="JQH51_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY6075222.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="JQH51_000769"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="JQH51_000769"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY6075223.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="JQH51_000770"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="JQH51_000770"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY6075224.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="JQH51_000771"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="JQH51_000771"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY6075225.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="JQH51_000772"
     CDS             complement(35511..35606)
                     /locus_tag="JQH51_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075226.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="JQH51_000773"
     CDS             complement(35720..35887)
                     /locus_tag="JQH51_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075227.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="JQH51_000774"
     CDS             complement(35890..36054)
                     /locus_tag="JQH51_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY6075228.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="JQH51_000775"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="JQH51_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY6075229.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="JQH51_000776"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="JQH51_000776"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY6075230.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="JQH51_000777"
     CDS             40510..40761
                     /locus_tag="JQH51_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY6075231.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="JQH51_000778"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="JQH51_000778"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY6075232.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="JQH51_000779"
     CDS             42066..42824
                     /locus_tag="JQH51_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY6075233.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="JQH51_000780"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="JQH51_000780"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY6075234.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="JQH51_000781"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="JQH51_000781"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY6075235.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="JQH51_000782"
     CDS             complement(44424..45044)
                     /locus_tag="JQH51_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY6075236.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="JQH51_000783"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="JQH51_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY6075237.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="JQH51_000784"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="JQH51_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY6075238.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="JQH51_000785"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="JQH51_000785"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY6075239.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="JQH51_000786"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="JQH51_000786"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY6075240.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49357..50715
                     /gene="trpCF"
                     /locus_tag="JQH51_000787"
     CDS             49357..50715
                     /gene="trpCF"
                     /locus_tag="JQH51_000787"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY6075241.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="JQH51_000788"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="JQH51_000788"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY6075242.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="JQH51_000789"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="JQH51_000789"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY6075243.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="JQH51_000790"
     CDS             53106..53285
                     /locus_tag="JQH51_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY6075244.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="JQH51_000791"
     CDS             53371..53871
                     /locus_tag="JQH51_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY6075245.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="JQH51_000792"
     CDS             53917..54423
                     /locus_tag="JQH51_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY6075246.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="JQH51_000793"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="JQH51_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY6075247.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="JQH51_000794"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="JQH51_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="JQH51_000795"
     CDS             55478..56221
                     /locus_tag="JQH51_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY6075248.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="JQH51_000796"
     CDS             56251..56790
                     /locus_tag="JQH51_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY6075249.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="JQH51_000797"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="JQH51_000797"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY6075250.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="JQH51_000798"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="JQH51_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY6075251.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="JQH51_000799"
     CDS             58276..58572
                     /locus_tag="JQH51_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY6075252.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14061 a        15144 c        14644 g        14724 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DADLEE010000012        58031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000012 DADLEE010000000
VERSION     DADLEE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58031
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.012"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(24..278)
                     /locus_tag="JQH51_000800"
     CDS             complement(24..278)
                     /locus_tag="JQH51_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY6075253.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JQH51_000801"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JQH51_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY6075254.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JQH51_000802"
     CDS             1228..2694
                     /locus_tag="JQH51_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY6075255.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JQH51_000803"
     CDS             2812..3798
                     /locus_tag="JQH51_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY6075256.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JQH51_000804"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JQH51_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY6075257.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JQH51_000805"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JQH51_000805"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY6075258.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JQH51_000806"
     CDS             5709..7265
                     /locus_tag="JQH51_000806"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY6075259.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JQH51_000807"
     CDS             7347..9161
                     /locus_tag="JQH51_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY6075260.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JQH51_000808"
     CDS             9162..10256
                     /locus_tag="JQH51_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY6075261.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JQH51_000809"
     CDS             10256..11281
                     /locus_tag="JQH51_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6075262.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JQH51_000810"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JQH51_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY6075263.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JQH51_000811"
     CDS             complement(12876..13220)
                     /locus_tag="JQH51_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075264.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQH51_000812"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQH51_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY6075265.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQH51_000813"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQH51_000813"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY6075266.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JQH51_000814"
     CDS             15616..17376
                     /locus_tag="JQH51_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY6075267.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JQH51_000815"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JQH51_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY6075268.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQH51_000816"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQH51_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY6075269.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JQH51_000817"
     CDS             19113..19340
                     /locus_tag="JQH51_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY6075270.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JQH51_000818"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JQH51_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY6075271.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JQH51_000819"
     tRNA            21195..21271
                     /locus_tag="JQH51_000819"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JQH51_000820"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JQH51_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY6075272.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JQH51_000821"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JQH51_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY6075273.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JQH51_000822"
     CDS             complement(25143..26195)
                     /locus_tag="JQH51_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY6075274.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JQH51_000823"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JQH51_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY6075275.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JQH51_000824"
     CDS             complement(26746..28689)
                     /locus_tag="JQH51_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY6075276.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JQH51_000825"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JQH51_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY6075277.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JQH51_000826"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JQH51_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY6075278.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JQH51_000827"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JQH51_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY6075279.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JQH51_000828"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JQH51_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY6075280.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JQH51_000829"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JQH51_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY6075281.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JQH51_000830"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JQH51_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY6075282.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JQH51_000831"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JQH51_000831"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY6075283.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JQH51_000832"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JQH51_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY6075284.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JQH51_000833"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JQH51_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY6075285.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JQH51_000834"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JQH51_000834"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY6075286.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JQH51_000835"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JQH51_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY6075287.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JQH51_000836"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JQH51_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY6075288.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="JQH51_000837"
     CDS             37391..37555
                     /locus_tag="JQH51_000837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075289.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JQH51_000838"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JQH51_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY6075290.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="JQH51_000839"
     CDS             complement(38300..39331)
                     /locus_tag="JQH51_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY6075291.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="JQH51_000840"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="JQH51_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="JQH51_000841"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="JQH51_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY6075292.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="JQH51_000842"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="JQH51_000842"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY6075293.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="JQH51_000843"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="JQH51_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY6075294.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="JQH51_000844"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="JQH51_000844"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY6075295.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="JQH51_000845"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="JQH51_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY6075296.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="JQH51_000846"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="JQH51_000846"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY6075297.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="JQH51_000847"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="JQH51_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY6075298.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="JQH51_000848"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="JQH51_000848"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY6075299.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="JQH51_000849"
     CDS             complement(53338..54114)
                     /locus_tag="JQH51_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY6075300.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="JQH51_000850"
     CDS             complement(54119..55768)
                     /locus_tag="JQH51_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY6075301.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="JQH51_000851"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="JQH51_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56487..>58031)
                     /locus_tag="JQH51_000852"
     CDS             complement(56487..>58031)
                     /locus_tag="JQH51_000852"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY6075302.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15655 c        14257 g        13921 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtagc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga cgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta a
//
LOCUS       DADLEE010000013        55613 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000013 DADLEE010000000
VERSION     DADLEE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55613
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.013"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..441)
                     /locus_tag="JQH51_000853"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="JQH51_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="JQH51_000854"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="JQH51_000854"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY6075303.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2372)
                     /locus_tag="JQH51_000855"
     CDS             complement(1383..2372)
                     /locus_tag="JQH51_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY6075304.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /locus_tag="JQH51_000856"
     CDS             complement(2384..3898)
                     /locus_tag="JQH51_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY6075305.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /locus_tag="JQH51_000857"
                     /pseudo
     CDS             complement(3968..4957)
                     /locus_tag="JQH51_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            5754..6257
                     /locus_tag="JQH51_000858"
     CDS             5754..6257
                     /locus_tag="JQH51_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY6075306.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="JQH51_000859"
     CDS             complement(6337..6588)
                     /locus_tag="JQH51_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY6075307.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="JQH51_000860"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="JQH51_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY6075308.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="JQH51_000861"
     CDS             7052..7375
                     /locus_tag="JQH51_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075309.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="JQH51_000862"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="JQH51_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY6075310.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="JQH51_000863"
     CDS             complement(8080..8319)
                     /locus_tag="JQH51_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY6075311.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="JQH51_000864"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="JQH51_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY6075312.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="JQH51_000865"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="JQH51_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY6075313.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="JQH51_000866"
     tRNA            complement(10643..10729)
                     /locus_tag="JQH51_000866"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="JQH51_000867"
     tRNA            complement(10742..10815)
                     /locus_tag="JQH51_000867"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="JQH51_000868"
     tRNA            complement(10870..10945)
                     /locus_tag="JQH51_000868"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="JQH51_000869"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="JQH51_000869"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY6075314.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="JQH51_000870"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="JQH51_000870"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY6075315.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="JQH51_000871"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="JQH51_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY6075316.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="JQH51_000872"
     CDS             14483..14659
                     /locus_tag="JQH51_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075317.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="JQH51_000873"
     CDS             14646..14870
                     /locus_tag="JQH51_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY6075318.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="JQH51_000874"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="JQH51_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="JQH51_000875"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="JQH51_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY6075319.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="JQH51_000876"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="JQH51_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY6075320.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="JQH51_000877"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="JQH51_000877"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY6075321.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="JQH51_000878"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="JQH51_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6075322.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="JQH51_000879"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="JQH51_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY6075323.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /locus_tag="JQH51_000880"
                     /pseudo
     CDS             complement(19761..20480)
                     /locus_tag="JQH51_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /locus_tag="JQH51_000881"
     CDS             complement(20645..22297)
                     /locus_tag="JQH51_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HAY6075324.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="JQH51_000882"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="JQH51_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY6075325.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="JQH51_000883"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="JQH51_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY6075326.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="JQH51_000884"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="JQH51_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY6075327.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="JQH51_000885"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="JQH51_000885"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY6075328.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="JQH51_000886"
     CDS             complement(26368..26781)
                     /locus_tag="JQH51_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6075329.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="JQH51_000887"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="JQH51_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY6075330.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="JQH51_000888"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="JQH51_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY6075331.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="JQH51_000889"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="JQH51_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..30428)
                     /gene="tnpB"
                     /locus_tag="JQH51_000890"
                     /pseudo
     CDS             complement(29220..30428)
                     /gene="tnpB"
                     /locus_tag="JQH51_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            30496..31111
                     /gene="tnpA"
                     /locus_tag="JQH51_000891"
                     /pseudo
     CDS             30496..31111
                     /gene="tnpA"
                     /locus_tag="JQH51_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31439..32521
                     /locus_tag="JQH51_000892"
     CDS             31439..32521
                     /locus_tag="JQH51_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY6075332.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(33010..33808)
                     /locus_tag="JQH51_000893"
                     /pseudo
     CDS             complement(33010..33808)
                     /locus_tag="JQH51_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="JQH51_000894"
     CDS             complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="JQH51_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY6075333.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="JQH51_000895"
     CDS             complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="JQH51_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY6075334.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="JQH51_000896"
     CDS             complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="JQH51_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY6075335.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            35743..37402
                     /gene="fliF"
                     /locus_tag="JQH51_000897"
                     /pseudo
     CDS             35743..37402
                     /gene="fliF"
                     /locus_tag="JQH51_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            37395..38390
                     /gene="fliG"
                     /locus_tag="JQH51_000898"
     CDS             37395..38390
                     /gene="fliG"
                     /locus_tag="JQH51_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY6075336.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            38383..39069
                     /gene="fliH"
                     /locus_tag="JQH51_000899"
     CDS             38383..39069
                     /gene="fliH"
                     /locus_tag="JQH51_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY6075337.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            39069..40442
                     /gene="fliI"
                     /locus_tag="JQH51_000900"
     CDS             39069..40442
                     /gene="fliI"
                     /locus_tag="JQH51_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY6075338.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            40464..40805
                     /gene="fliJ"
                     /locus_tag="JQH51_000901"
                     /pseudo
     CDS             40464..40805
                     /gene="fliJ"
                     /locus_tag="JQH51_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            40802..41929
                     /gene="fliK"
                     /locus_tag="JQH51_000902"
     CDS             40802..41929
                     /gene="fliK"
                     /locus_tag="JQH51_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY6075339.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            42034..42498
                     /gene="fliL"
                     /locus_tag="JQH51_000903"
     CDS             42034..42498
                     /gene="fliL"
                     /locus_tag="JQH51_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY6075340.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            42503..43507
                     /gene="fliM"
                     /locus_tag="JQH51_000904"
     CDS             42503..43507
                     /gene="fliM"
                     /locus_tag="JQH51_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY6075341.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            43504..43917
                     /gene="fliN"
                     /locus_tag="JQH51_000905"
     CDS             43504..43917
                     /gene="fliN"
                     /locus_tag="JQH51_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY6075342.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            43920..44285
                     /gene="fliO"
                     /locus_tag="JQH51_000906"
     CDS             43920..44285
                     /gene="fliO"
                     /locus_tag="JQH51_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY6075343.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            44285..45022
                     /gene="fliP"
                     /locus_tag="JQH51_000907"
     CDS             44285..45022
                     /gene="fliP"
                     /locus_tag="JQH51_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY6075344.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            45032..45301
                     /gene="fliQ"
                     /locus_tag="JQH51_000908"
     CDS             45032..45301
                     /gene="fliQ"
                     /locus_tag="JQH51_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY6075345.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            45310..46095
                     /gene="fliR"
                     /locus_tag="JQH51_000909"
     CDS             45310..46095
                     /gene="fliR"
                     /locus_tag="JQH51_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY6075346.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            46380..47003
                     /gene="rcsA"
                     /locus_tag="JQH51_000910"
     CDS             46380..47003
                     /gene="rcsA"
                     /locus_tag="JQH51_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY6075347.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(47047..47289)
                     /gene="dsrB"
                     /locus_tag="JQH51_000911"
     CDS             complement(47047..47289)
                     /gene="dsrB"
                     /locus_tag="JQH51_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY6075348.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            47398..47625
                     /gene="yodD"
                     /locus_tag="JQH51_000912"
     CDS             47398..47625
                     /gene="yodD"
                     /locus_tag="JQH51_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY6075349.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            47923..48738
                     /locus_tag="JQH51_000913"
                     /pseudo
     CDS             47923..48738
                     /locus_tag="JQH51_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(48735..50429)
                     /gene="yedQ"
                     /locus_tag="JQH51_000914"
                     /pseudo
     CDS             complement(48735..50429)
                     /gene="yedQ"
                     /locus_tag="JQH51_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(50600..50782)
                     /locus_tag="JQH51_000915"
     CDS             complement(50600..50782)
                     /locus_tag="JQH51_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY6075350.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(50861..51778)
                     /locus_tag="JQH51_000916"
     CDS             complement(50861..51778)
                     /locus_tag="JQH51_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY6075351.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            51951..52871
                     /gene="yedA"
                     /locus_tag="JQH51_000917"
     CDS             51951..52871
                     /gene="yedA"
                     /locus_tag="JQH51_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY6075352.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(52860..53330)
                     /locus_tag="JQH51_000918"
     CDS             complement(52860..53330)
                     /locus_tag="JQH51_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY6075353.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(53311..54729)
                     /locus_tag="JQH51_000919"
     CDS             complement(53311..54729)
                     /locus_tag="JQH51_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY6075354.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(54796..55336)
                     /locus_tag="JQH51_000920"
                     /pseudo
     CDS             complement(54796..55336)
                     /locus_tag="JQH51_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(55376..>55613)
                     /locus_tag="JQH51_000921"
     CDS             complement(55376..>55613)
                     /locus_tag="JQH51_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075355.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        13906 a        14313 c        13455 g        13939 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccacata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
    29701 gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat
    29761 ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa
    29821 ctgtttacag gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca
    29881 tccagcccga ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga
    29941 atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg
    30001 acctgacggc tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt
    30061 tcctgagcga gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg
    30121 aatcagggga acgccgagcg cttctggaaa aagtttttgc aggcctgctc agggtctttc
    30181 agtgactgtt gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt
    30241 ttccactcaa ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta
    30301 tgattctcat tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag
    30361 cgcctcattt cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt
    30421 cgtttcacag ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg
    30481 cactgtgttt tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt
    30541 tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg tgcttatttt
    30601 gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatctgtt aattaactat
    30661 ccccccaaac tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag
    30721 gttactgcga cgggatcgac cagatattgc tgccaggtat taccgcaaag atgttttgtg
    30781 gcgtcccggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    30841 cattgaacaa cagcaaacac ctggtcagat gaaaaaacga gccttatatc cccgccctga
    30901 aggacgggct tttacggtgc actggataag cctggagatt atctacacct gcatggacat
    30961 cattgtacta tgccccgtac atgctccatc acatcctcaa catcaaagta ttagagcctt
    31021 gcaaaccatt atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat
    31081 atcaagcggc gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa
    31141 ataaatatca tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa
    31201 attatgagtc aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg
    31261 ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta
    31321 cttcacaatt taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg
    31381 aatatttgta gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat
    31441 gaaaaaatta acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc
    31501 tcaggcagca gagatttaca ataaagacag taacaagctg gatctgtacg gaaaagttaa
    31561 tgctaagcac tacttctctt ctaacgatgc agatgatggc gacacaactt atgttcgtct
    31621 gggcttcaaa ggcgaaactc aaatcaacga tcaactgact ggtttcggtc agtgggaata
    31681 tgaattcaaa ggcaaccgtg ctgaatctca aggttcttcc aaagataaaa cccgtcttgc
    31741 cttcgctggc ctgaaattcg gtgactacgg ctccatcgat tatggccgta actacggtgt
    31801 agcatacgat atcggtgcat ggaccgacgt tctgccagaa ttcggtggcg atacctggac
    31861 ccaaacagat gtgttcatga ctggtcgcac cacaggtgtt gcaacctatc gtaacaacga
    31921 cttctttggt ctggttgatg gtctgaactt tgctgctcag tatcagggta aaaatgaccg
    31981 cactgacgta actgaagcta acggtgatgg tttcggtttc tccactactt atgagtatga
    32041 aggattcggt gtaggtgcaa cctatgctaa atctgaccgc actaatgatc aggttatcta
    32101 cggtaacaac agcctgaatg catctggtca aaatgctgaa gtatgggcag ctggtctgaa
    32161 atatgatgcg aacaacatct atctggctac cacctattct gaaacccaga acatgactgt
    32221 ttttggtaat aaccatattg ccaacaaagc acaaaacttc gaagtagttg cacaatatca
    32281 gttcgacttc ggtctgcgtc cgtccgttgc ttacctgcaa tctaaaggaa aagacttggg
    32341 tgcgtggggt gatcaggacc tgattgaata tattgatgta ggtgcaacct attacttcaa
    32401 caaaaatatg tccacttttg ttgattacaa aatcaacctg attgataaga gcgatttcac
    32461 gaaagcatct ggcgttgcta ccgatgatat cgttgctgta ggattggttt accagttcta
    32521 atctgattac tcaaagatat gttgtgggag gctttgcctc cccaacatat aagtggcgct
    32581 ctcaagccac tttccttaga agcactatct tgcttctgac tatataaatt accctttatt
    32641 ttctgggacg tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat
    32701 tagatttaat aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt
    32761 taattatccc ctcaacctct tgtaaatttc ccactcttga gctccacata gcccaaacgc
    32821 cggatgcaaa aaaaatcata cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa
    32881 ttatatgtgc ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca
    32941 ataggttgag tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat
    33001 aaaatacttt tactctctgg acgcttttct tagctctctt ggtgttatat taaaacgatt
    33061 atggaacctt tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct
    33121 gatgggtttt actgtcgttt gtatagcagg acaacgcatt atttaacctc acgtcgtcca
    33181 atatactttg aaaacttatt ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa
    33241 tgtagagata tctcgctacc ttgtttgctg tccattttgt tctaggttcg gatagcatta
    33301 ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa caattaacat
    33361 gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga
    33421 agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg gggttctgag
    33481 gtaagcagaa acctgtcgtg ttacgaattt gatcagttat cgcataaaat ttttgaaact
    33541 tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct gcttctggat
    33601 aattcacaat ataaaattca tatccacagg gaaataatat tatctgatta ttatcaacag
    33661 ttagactatg cgtctcccaa ttgataactt tttttccctg acggatacga cataaagctg
    33721 gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct
    33781 ttaggttaga cttatctctt gccagcatac tcttacccta ttttatctca taaactggtg
    33841 ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg
    33901 aattagaatc cacaggaatc ttatcagttt ttagagccca tactttaaac tggtaatgat
    33961 ggggtttatc tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc
    34021 ggccttgaac agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagtgggca
    34081 aatatgttac agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag
    34141 gagcatctgg atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc
    34201 aggttaatga gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg
    34261 ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcctgcg
    34321 cagaaaatgt tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat
    34381 tgttgtgacc tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca
    34441 atgatcgcgc aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca
    34501 caaatcagat tcctgttatc tcaaggagaa ttatttttta atgcggtgca cttcgtgaca
    34561 acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt
    34621 ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgactcctg
    34681 accagatagc ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga
    34741 atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg
    34801 aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat
    34861 aagggttcat agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac
    34921 acaactcacc gttttcatcc tggtgtatgt gtatccaagg ggctctggcg tggcaagaga
    34981 taaaggcagg aacaataatg atcagaagga accccaggct aacgaatgag ttcactcttc
    35041 ctgggagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa
    35101 caaccaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc catccatgcc
    35161 ccacgctgtg ggatcggata taattaaaca agcatagtca acaggttaca caacctacac
    35221 ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
    35281 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg
    35341 ttcaccgagg gtgaattttt ctgcctgcgt gcgggcgact gtttgtgtat cgcttatgcg
    35401 atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg
    35461 cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat
    35521 cgctgacatt ttcgtctccc ggataatttc tggtaacaaa gcctaccagt aagtcaataa
    35581 gacaaaggcg ctaaatagca acaaaaaaac tggtttattg gcggatagaa aaaacgaaag
    35641 cacaaataat gggagcgtca atttttcgag tgtgctgacc cgagagtgag tcttgttcca
    35701 ctttgccaat aacgccgtcc ataatcagcc acgaagtgcg cgatgaatgc gactgcagcc
    35761 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    35821 gttaccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
    35881 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
    35941 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaggttccg
    36001 gcagataaag ttcacgaact gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
    36061 ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
    36121 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
    36181 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
    36241 ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt
    36301 agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgcctgg aaacgtcacg
    36361 ctggtggatc agggcgggca tctgttaacc cagtccaata ccagcgggcg cgatcttaat
    36421 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcagcg tattgaagcg
    36481 atcctgtcac ctattgttgg taacggtaat attcacgcct aggtcacggc gcaactggac
    36541 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
    36601 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
    36661 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
    36721 ccgacaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag tgggccgcgt
    36781 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
    36841 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg
    36901 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agctctgacc
    36961 cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt taactcgccg
    37021 ttcaatagca gtgacgaaag cggcggagca ctgccgtttt ggcaacagca agtgtttatc
    37081 gatcagttgc tggctgccgg tcgctggttg ctggttgctg gtactgctgg tggcgtggtt
    37141 gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc gccgaggcgg tgaagactgt
    37201 gcagcaacag gcgcaggccc gcgaggaagt ggaagatgcg gtggaagtcc gtctgagcaa
    37261 agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg ggggcagaag tcatgagcca
    37321 gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg
    37381 gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc ctgctgatga
    37441 ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc gaagtgcaaa
    37501 ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc
    37561 tggcggagtt tgagcaagaa gctgaacagt ttgctgcact gaatatcaac gccaacgatt
    37621 atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc ctgctggaag
    37681 atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc
    37741 agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc attctggtgc
    37801 atctgaggcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg
    37861 acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gccgaactga
    37921 ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg
    37981 tgagaacggc agccgaaatt atcaacctga tgaaaacgca gcaggaagaa gccgttatta
    38041 ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag atgttcctgt
    38101 tcgagaatct ggtggatgtc gatgatcgca gcattcagcg tctgttgcag gaagtggatt
    38161 ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc
    38221 gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaag cgtggtccgg
    38281 tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct gctgattgtg cgccgtcttg
    38341 ccgaaactgg cgagatggtg attggcagcg gcgaggatac ctatgtctga taatctgccg
    38401 tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg cagagtttgt gccaatggtc
    38461 gagtcggaag aaaccattat tgaagaggcc gaacccagcc ttgagcagca actggcgcaa
    38521 ctgcaaatgc aggcccacga gcagggttat caggcgggga ttgccgaagg tcgccagcaa
    38581 ggtcacgaac agggctatca ggaaggactg gcccaggggc tggaacaagg tctggcagag
    38641 gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa
    38701 actacccttg atgcgcttga tagtgtgata gcttcgcgcc tgatgcagat ggcgctggag
    38761 gcagcacgtc aggtgatcgg tcagacacca acggtggata actcggcact gatcaaacag
    38821 atccaacagt tgttgcagca agaaccgtta ttcagcggga aaccacagct gcgcgtacac
    38881 ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ctttaagttt gcatggctgg
    38941 cgcttgcggg gcgatcccac cctccaccct ggcggatgta aagtctccgc cgatgaaggc
    39001 gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga
    39061 gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca
    39121 aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct accgggctgg
    39181 tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt gagcgccaga
    39241 acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt
    39301 ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgta tatgccaaaa
    39361 acatttcggc agaagggctg caaagcggca agcagttgcc gctcggtccg gcgttattag
    39421 gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct gcccttcccc gatacgacgg
    39481 aaaccggtgc gctgattacc ccgccattta acccgttgca acgtacaccg attgaacatg
    39541 tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcggacgc gggcagcgta
    39601 tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct gctggggatg atggcacgct
    39661 acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag
    39721 attttattga gaatatcctc ggtgccgaag gacgtgcacg ctcagtggtg attgccgctc
    39781 cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc ctatgccacg cgcattgccg
    39841 aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg
    39901 cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acctgccact aaaggttatc
    39961 caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggtaat ggcattagcg
    40021 gcggcggctc gattaccgcg ttttataccg tgctcaccga aggcgatgac cagcaggacc
    40081 ccattgctga ctccgcgcgg gcaatcctcg acggtcacat tgtgctgtct cgccgactgg
    40141 cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagtcgc gcaatgacgg
    40201 cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt
    40261 ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc
    40321 tcgataaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa ggcatttttg
    40381 aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtgtcat
    40441 aacccaggag ataacggcag atggcggaag aacagctcaa aatgttgatc gattatcaga
    40501 atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc aaccgctgga
    40561 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
    40621 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
    40681 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
    40741 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
    40801 aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat tgcctggcgg
    40861 caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat tagcaggcga
    40921 gacaacaact gacaaagcgg ccccccagtt gctggtagca acagataagc ccacgacaaa
    40981 aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt tactgatccc
    41041 tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac cgttaaccac
    41101 cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag acgaaaaagc
    41161 ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga tgttgccggg
    41221 ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg cagagaaacc
    41281 aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg atgacgcccc
    41341 cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
    41401 catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc cacaccagac
    41461 acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt ctcacgaatg
    41521 gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac aaagtgcaga
    41581 gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag tggatgataa
    41641 ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc tggaagcagc
    41701 gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc aaagcaacat
    41761 tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc aaagccaacg
    41821 cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgtctc
    41881 tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac gtcagaggta
    41941 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
    42001 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
    42061 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
    42121 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
    42181 gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt gggcgatgcg
    42241 gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac ccgctcgcgg
    42301 ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc acgtcaggat
    42361 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccaca
    42421 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
    42481 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
    42541 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtatta gcggcgaaag
    42601 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
    42661 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt tcaacctgct
    42721 gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccgtacc atgaatttgc
    42781 ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc tacgcggcac
    42841 agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
    42901 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg aacagcgcgt
    42961 catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga aagcgattaa
    43021 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca atatcaccac
    43081 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
    43141 cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat tgttggttaa
    43201 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
    43261 ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc tacgcctgtc
    43321 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg atcgcatcat
    43381 cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggtactctca acggtcagta
    43441 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
    43501 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
    43561 gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga cgcggtgttc
    43621 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gattatggat
    43681 attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa agagctgttg
    43741 cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc gctggatatt
    43801 ctgatcaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga taaatatggc
    43861 gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag ccgttagtga
    43921 tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca ctgctgcagg
    43981 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
    44041 ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
    44101 cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
    44161 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
    44221 agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt ggcgggagat
    44281 cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta cgcccctcgc
    44341 cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac aaagctggtc
    44401 gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag cgattttact
    44461 gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg
    44521 aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt tgaccttttt
    44581 tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga
    44641 gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct
    44701 gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata ccggcccgtt
    44761 gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt
    44821 gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt
    44881 gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca ccattgctct
    44941 gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct
    45001 ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg
    45061 aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg tagcgctggt
    45121 cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa tgacgctgtc
    45181 gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac cgtggatgct
    45241 caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta
    45301 gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag cctgtacttc
    45361 tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag cgaacgcagc
    45421 gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat tgccccatca
    45481 ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtacagcag
    45541 atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc tgtgcgaacc
    45601 gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt cgatcctggc
    45661 agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt actgctgttc
    45721 ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt tcacaccctg
    45781 ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa agcagggagt
    45841 ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt gctgaccctg
    45901 aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt tgttattgga
    45961 tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc gttaattgcg
    46021 cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat tattagtgaa
    46081 ttaccattaa tataattccg taacgtttat catgctaccc taaggattat ccgaaaaata
    46141 atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta agctcactca
    46201 catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact ttacgggata
    46261 gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt gttccgttta
    46321 gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca
    46381 tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt
    46441 tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca
    46501 tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt ttcatccacg
    46561 atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat acgttattta
    46621 tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaatt
    46681 tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc
    46741 tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa
    46801 ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc
    46861 aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca
    46921 aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg
    46981 gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga
    47041 ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt cattaaagaa
    47101 ccagatgccg agcgggtagt cttccagcgc aaccaggtac attgtgcctt cactaaactc
    47161 ctcaactgcc agtaccactc caggacgacg cggaccgcca tccgttttga ctgttacccg
    47221 atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt
    47281 ttctggcagc gccaagcgcg cgcgagtgct gattttctcg acggtctata cttaagagat
    47341 gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg
    47401 aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc
    47461 ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct
    47521 gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc
    47581 gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa
    47641 atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc
    47701 gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga
    47761 aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc
    47821 ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg
    47881 ctgttttgag aatcatcctg gtaaggggag tactgcgcga ccatgttttc aattcaacaa
    47941 ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg
    48001 caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt
    48061 agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta
    48121 ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac
    48181 ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg
    48241 ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt
    48301 gccgaatgga cgggattaag ccgtagctag gcggcgctga cgcagctgca tgaggcgtcg
    48361 gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac
    48421 gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc
    48481 ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcagacgc
    48541 ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat
    48601 tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga tgaggatccg
    48661 gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat
    48721 tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc ggcctgtttt
    48781 gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt
    48841 tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc
    48901 agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc
    48961 agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc
    49021 tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc
    49081 tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa
    49141 aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacagtgcg
    49201 ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc
    49261 agaacataca tgttgctgac catccggcga atcacatacc aggagaggag taacatggtg
    49321 gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg
    49381 ccttcgctta gcgtatggac gcgcgccagc acaccgtcga aatggtccag acgttcccag
    49441 ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc
    49501 agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag
    49561 gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg
    49621 gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac
    49681 cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg
    49741 tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg
    49801 ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac
    49861 aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc ctgttcaacc
    49921 atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt
    49981 tcattgtcga ggctttcatt ttcgcgagac aggaaattcc cctcgctgac taatgcatcc
    50041 gatacgccat tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg
    50101 tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc
    50161 acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa
    50221 tgcttatcga gcgcgttggc tacgttttcc agatgattac gctagctgga gatataggca
    50281 tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata
    50341 aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc
    50401 tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat
    50461 tctggcataa cccggcaata gcgtaccgca aaacagcgat aattttataa tggtggcatg
    50521 ataagacgcg actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc
    50581 tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa
    50641 gatcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa
    50701 ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt
    50761 cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta
    50821 gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca
    50881 ttttcgctac tgctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
    50941 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgatatgtt
    51001 caatagcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
    51061 tcgccagcgt gccgacaatc gataatgctt tcatcagcca aggcgcgata atcaataacc
    51121 ctttacctaa tgcctgcatc agcgcgctag atttttccgc cagccaatac cctaggtcat
    51181 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
    51241 ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg
    51301 cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt
    51361 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgctggat
    51421 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga
    51481 gaaacgcgcc accaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga
    51541 tcagcgccag cggcaccaga atcactttat tgatcagcga tcctttcgcc acgccccaga
    51601 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataaat
    51661 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcaca gagatatcgt
    51721 ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
    51781 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
    51841 gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat
    51901 gattttcacg tttactatga accacctttt tatttccgcc gtgagggatt atgcgtttcc
    51961 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
    52021 attttgtcat tcggattggc gtgggaagct ggcctccgtt aatgatggcg ggcgttcgat
    52081 tccaggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc
    52141 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
    52201 gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtgattg
    52261 caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa acgcgcaaac
    52321 tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
    52381 atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
    52441 ttggctcagt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg
    52501 agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt gaaaaactga
    52561 cggcgctccc ttccctgtca ggcttccttg cggtcggcta tctggcgctg tttggttcga
    52621 ttatcgccat taacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca
    52681 gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa
    52741 cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca
    52801 cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat
    52861 caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc
    52921 cactcttcca gacgctcggt aagcgccgca tccgtcagct tctcgcgcct acgtaacgca
    52981 cactcccaga caatcagtac gcgccagccg agctcctgca agcgactgat atcgcggcga
    53041 tcgcgttcaa catttttacc tatcttctcc agccagaact cagttcgagt cgcaggcact
    53101 ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga
    53161 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca
    53221 aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca
    53281 atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc
    53341 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
    53401 aaagaccggc acgaccaccg agttgccgaa ctggcgatag gcctgagtgt ccgaaaccgg
    53461 aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg
    53521 cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt
    53581 ggccatatcc caaccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgagcaga
    53641 cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
    53701 accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
    53761 gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg
    53821 gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa
    53881 acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat cgatgatttt
    53941 cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca tagcccagtt cgtccagcgt
    54001 ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacgttttc
    54061 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
    54121 gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
    54181 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
    54241 ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat
    54301 gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata
    54361 agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccac caatcgattc
    54421 aaaaccacga cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg
    54481 cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact
    54541 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
    54601 attctcaccc acaccattaa gctgcgccac cagcatttta acatcataaa tttgcagcag
    54661 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
    54721 ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg
    54781 tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc
    54841 ttcattctcc cccgccagtt cggcaccgag cttcgccatc aaacagcgcc aagctcaatg
    54901 cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg
    54961 catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag
    55021 cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcac
    55081 ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat
    55141 ttttcgccag gctcacgata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga
    55201 catcgtcatc tgccgccagt ttctgtgccg tagcccagac acggcgaaaa tgacacaggt
    55261 catgcgccgc gtcctgatgt tgatgatggt ttttcaacca gttctcgaac tatgcctgcc
    55321 agtgttgtaa ctccatacgc gctccgttgc caaagagtct caaccttagc agtttttatt
    55381 catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca tgcaatgccg cctaacaagg
    55441 caccaaccag ggccataaac agaaatccga ccaatgctcc taaccacttc cccgcggtag
    55501 atcctaaatc actttcaaat cccgacacgg gggcatcggg gatccggctc atcacccaga
    55561 tgtatcgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc cac
//
LOCUS       DADLEE010000014        55418 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000014 DADLEE010000000
VERSION     DADLEE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.014"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQH51_000922"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQH51_000922"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY6075356.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQH51_000923"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQH51_000923"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY6075357.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQH51_000924"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQH51_000924"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY6075358.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JQH51_000925"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JQH51_000925"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY6075359.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JQH51_000926"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JQH51_000926"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY6075360.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JQH51_000927"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JQH51_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY6075361.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JQH51_000928"
     CDS             9708..10559
                     /locus_tag="JQH51_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6075362.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JQH51_000929"
     CDS             complement(10546..10887)
                     /locus_tag="JQH51_000929"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY6075363.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JQH51_000930"
     CDS             complement(10889..11767)
                     /locus_tag="JQH51_000930"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY6075364.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JQH51_000931"
     CDS             complement(11733..14030)
                     /locus_tag="JQH51_000931"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY6075365.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILCRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JQH51_000932"
     CDS             complement(14081..14401)
                     /locus_tag="JQH51_000932"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY6075366.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JQH51_000933"
     CDS             complement(14416..15495)
                     /locus_tag="JQH51_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY6075367.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="JQH51_000934"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="JQH51_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JQH51_000935"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JQH51_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="JQH51_000936"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="JQH51_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="JQH51_000937"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="JQH51_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21938)
                     /gene="yijE"
                     /locus_tag="JQH51_000938"
     CDS             complement(21000..21938)
                     /gene="yijE"
                     /locus_tag="JQH51_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY6075368.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="JQH51_000939"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="JQH51_000939"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY6075369.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="JQH51_000940"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="JQH51_000940"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY6075370.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /locus_tag="JQH51_000941"
     CDS             complement(25747..28179)
                     /locus_tag="JQH51_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY6075371.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="JQH51_000942"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="JQH51_000942"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY6075372.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="JQH51_000943"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="JQH51_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY6075373.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="JQH51_000944"
     CDS             30120..30728
                     /locus_tag="JQH51_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY6075374.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="JQH51_000945"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="JQH51_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY6075375.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="JQH51_000946"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="JQH51_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY6075376.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="JQH51_000947"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="JQH51_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY6075377.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="JQH51_000948"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="JQH51_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY6075378.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="JQH51_000949"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="JQH51_000949"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY6075379.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="JQH51_000950"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="JQH51_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY6075380.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="JQH51_000951"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="JQH51_000951"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY6075381.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="JQH51_000952"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="JQH51_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY6075382.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="JQH51_000953"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="JQH51_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY6075383.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="JQH51_000954"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="JQH51_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="JQH51_000955"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="JQH51_000955"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY6075384.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="JQH51_000956"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="JQH51_000956"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY6075385.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="JQH51_000957"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="JQH51_000957"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY6075386.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="JQH51_000958"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="JQH51_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY6075387.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="JQH51_000959"
     CDS             complement(44843..45142)
                     /locus_tag="JQH51_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY6075388.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="JQH51_000960"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="JQH51_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="JQH51_000961"
     CDS             45895..46494
                     /locus_tag="JQH51_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY6075389.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="JQH51_000962"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="JQH51_000962"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY6075390.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="JQH51_000963"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="JQH51_000963"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY6075391.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /locus_tag="JQH51_000964"
     CDS             complement(48286..49275)
                     /locus_tag="JQH51_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6075392.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="JQH51_000965"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="JQH51_000965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY6075393.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="JQH51_000966"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="JQH51_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY6075394.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="JQH51_000967"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="JQH51_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY6075395.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="JQH51_000968"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="JQH51_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY6075396.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="JQH51_000969"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="JQH51_000969"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY6075397.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="JQH51_000970"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="JQH51_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY6075398.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcacaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DADLEE010000015        55128 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000015 DADLEE010000000
VERSION     DADLEE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55128
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.015"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..354
                     /locus_tag="JQH51_000971"
     CDS             <1..354
                     /locus_tag="JQH51_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075399.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JQH51_000972"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JQH51_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY6075400.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JQH51_000973"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JQH51_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY6075401.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3052..3261
                     /gene="mokC"
                     /locus_tag="JQH51_000974"
     CDS             3052..3261
                     /gene="mokC"
                     /locus_tag="JQH51_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY6075402.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JQH51_000975"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JQH51_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY6075403.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JQH51_000976"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JQH51_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY6075404.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="JQH51_000977"
     CDS             6877..7281
                     /locus_tag="JQH51_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY6075405.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="JQH51_000978"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="JQH51_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY6075406.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="JQH51_000979"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="JQH51_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY6075407.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JQH51_000980"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JQH51_000980"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY6075408.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JQH51_000981"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JQH51_000981"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY6075409.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="JQH51_000982"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="JQH51_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="JQH51_000983"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="JQH51_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY6075410.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="JQH51_000984"
     CDS             complement(13133..13429)
                     /locus_tag="JQH51_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY6075411.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="JQH51_000985"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="JQH51_000985"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY6075412.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="JQH51_000986"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="JQH51_000986"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY6075413.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="JQH51_000987"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="JQH51_000987"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY6075414.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="JQH51_000988"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="JQH51_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY6075415.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="JQH51_000989"
     CDS             complement(18686..19372)
                     /locus_tag="JQH51_000989"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY6075416.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="JQH51_000990"
     CDS             complement(19772..19912)
                     /locus_tag="JQH51_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075417.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="JQH51_000991"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="JQH51_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY6075418.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="JQH51_000992"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="JQH51_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY6075419.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="JQH51_000993"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="JQH51_000993"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY6075420.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="JQH51_000994"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="JQH51_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY6075421.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="JQH51_000995"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="JQH51_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY6075422.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="JQH51_000996"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="JQH51_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY6075423.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="JQH51_000997"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="JQH51_000997"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY6075424.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="JQH51_000998"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="JQH51_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="JQH51_000999"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="JQH51_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY6075425.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="JQH51_001000"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="JQH51_001000"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY6075426.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="JQH51_001001"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="JQH51_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY6075427.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="JQH51_001002"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="JQH51_001002"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY6075428.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="JQH51_001003"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="JQH51_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY6075429.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="JQH51_001004"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="JQH51_001004"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY6075430.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="JQH51_001005"
     CDS             35470..36114
                     /locus_tag="JQH51_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY6075431.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="JQH51_001006"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="JQH51_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="JQH51_001007"
     CDS             37189..37452
                     /locus_tag="JQH51_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6075432.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="JQH51_001008"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="JQH51_001008"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY6075433.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="JQH51_001009"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="JQH51_001009"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY6075434.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="JQH51_001010"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="JQH51_001010"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY6075435.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="JQH51_001011"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="JQH51_001011"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY6075436.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="JQH51_001012"
     CDS             42150..43691
                     /locus_tag="JQH51_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY6075437.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /gene="yjjW"
                     /locus_tag="JQH51_001013"
     CDS             43663..44526
                     /gene="yjjW"
                     /locus_tag="JQH51_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY6075438.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="JQH51_001014"
     CDS             complement(44740..45522)
                     /locus_tag="JQH51_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6075439.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="JQH51_001015"
     CDS             complement(45519..46592)
                     /locus_tag="JQH51_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY6075440.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="JQH51_001016"
     CDS             complement(46714..46875)
                     /locus_tag="JQH51_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY6075441.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="JQH51_001017"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="JQH51_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY6075442.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="JQH51_001018"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="JQH51_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY6075443.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="JQH51_001019"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="JQH51_001019"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY6075444.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="JQH51_001020"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="JQH51_001020"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY6075445.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="JQH51_001021"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="JQH51_001021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY6075446.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="JQH51_001022"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="JQH51_001022"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY6075447.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52601..52687
                     /locus_tag="JQH51_001023"
     tRNA            52601..52687
                     /locus_tag="JQH51_001023"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52635..52637,aa:Leu,seq:cag)
     gene            complement(52726..52962)
                     /locus_tag="JQH51_001024"
     CDS             complement(52726..52962)
                     /locus_tag="JQH51_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY6075448.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53103..53891
                     /gene="fhuF"
                     /locus_tag="JQH51_001025"
     CDS             53103..53891
                     /gene="fhuF"
                     /locus_tag="JQH51_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY6075449.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(53929..54606)
                     /gene="bglJ"
                     /locus_tag="JQH51_001026"
     CDS             complement(53929..54606)
                     /gene="bglJ"
                     /locus_tag="JQH51_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY6075450.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54564..>55128)
                     /locus_tag="JQH51_001027"
     CDS             complement(54564..>55128)
                     /locus_tag="JQH51_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6075451.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13192 a        14886 c        13744 g        13306 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaaccgcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt
    52621 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
    52681 cgcaccaatt atctttactt cctttcttgt ttcttccctg ttttgtcatc cctgattcaa
    52741 attcattatc atcagcatga tgccagcaat aagcgccagg cacgatggta gcaaaatgta
    52801 atgccgcaac tgtttgtgat acagcatcac cggcgtcaat actaatccca tcagaatgac
    52861 aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca gcaatcccgg
    52921 cagcaacact ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt
    52981 gataatgaaa accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt
    53041 gctatcgagt aaacctatcg ctaggattag caatcattat catttagatt actatcacga
    53101 ttatggccta tcgttccgca ccgctctatg aagatgtcat ctggcgaacg catctccagc
    53161 cgcaggatgc tgggcttgca caagccgtac gggcgatgat cgccaaacat cgtgaacatt
    53221 tgctggagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat
    53281 ggtcatcacc gaatgcgcta agttctctac tggcggtcta ttccgatcat atctatcgca
    53341 accaaccaac gatgatccgc gagaacaaac cgctgatctc actatgggca caatggtata
    53401 tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaaa gcattagatg
    53461 tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc tgtttctggg
    53521 tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt
    53581 taatcagcca ggcgctggtt ccggttgtgc aagcactaga agcgaccgga gagatcaacg
    53641 gcaaacttat ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc
    53701 aactgctcgg cgaggctact gttgaatcgc tgcgccatgc cctctttttt gagaaaacgc
    53761 tcacgaacgg tgaagataat ccactctggc gtaccgtggt actgcgcgac ggcctgctgg
    53821 tgcgccgcac ttgctgccag cgttatcgct taccggatgt gcagcaatgt ggctattgta
    53881 cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg
    53941 caacacatta cttgcttcgc aatgccgcat acataacaga atatcttcgg cctccaacaa
    54001 ccctgcatca gaactgacgc ccagtttcga catcacatta aatttatgcg ctcggatcgt
    54061 tttgatatta cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa
    54121 gcgcaatatt tctctctccg tcgggcttaa cgtccggctt tggttaatgt accactgatt
    54181 gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa
    54241 aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc
    54301 aatcagccga gcttcaatat catcatccac aataactaaa cgccgggtac gcggaaactt
    54361 aatcgccagc tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa
    54421 agaaaaaatg accgccgcaa acgagatccg cgacatggca tcctgaaatg actcctgact
    54481 ggtataggta tggagcttat aagggttgcc cgcaaacttt ctgaataaac tctcaatacc
    54541 gatactactc atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg
    54601 ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg
    54661 ctatttatcc atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg
    54721 tttttttggg cgctgatagt tttattactt ttcttaagca atgaagctat ctggttgatg
    54781 ccccatcctt tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag
    54841 cgatagctaa aatcatgagt ttcagtcatt gccggagagg tcagcatggc gctgatacgc
    54901 tctgcgtgcg tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca
    54961 gacaataacg tcgccgtagg gcacatgagc agttctacag cctgggaata ccaggagcgt
    55021 gacaccatga aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca
    55081 caaacagaac gtggatcttc actttcaccg gctaaatcag caatgact
//
LOCUS       DADLEE010000016        53211 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000016 DADLEE010000000
VERSION     DADLEE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.016"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(11..322)
                     /locus_tag="JQH51_001028"
     CDS             complement(11..322)
                     /locus_tag="JQH51_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY6075452.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JQH51_001029"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JQH51_001029"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JQH51_001030"
     CDS             701..1054
                     /locus_tag="JQH51_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY6075453.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQH51_001031"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQH51_001031"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY6075454.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JQH51_001032"
     CDS             complement(2810..3280)
                     /locus_tag="JQH51_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075455.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQH51_001033"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQH51_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQH51_001034"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQH51_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY6075456.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQH51_001035"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQH51_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY6075457.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JQH51_001036"
     CDS             complement(5283..8432)
                     /locus_tag="JQH51_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY6075458.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQH51_001037"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQH51_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY6075459.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JQH51_001038"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JQH51_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY6075460.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JQH51_001039"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JQH51_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY6075461.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQH51_001040"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQH51_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY6075462.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQH51_001041"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQH51_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY6075463.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JQH51_001042"
     CDS             14911..15288
                     /locus_tag="JQH51_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY6075464.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JQH51_001043"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JQH51_001043"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY6075465.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JQH51_001044"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JQH51_001044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY6075466.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JQH51_001045"
     CDS             18099..18428
                     /locus_tag="JQH51_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY6075467.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JQH51_001046"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JQH51_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY6075468.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JQH51_001047"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JQH51_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY6075469.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JQH51_001048"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JQH51_001048"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY6075470.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JQH51_001049"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JQH51_001049"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY6075471.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQH51_001050"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQH51_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /locus_tag="JQH51_001051"
     CDS             24044..25348
                     /locus_tag="JQH51_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY6075472.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="JQH51_001052"
     CDS             complement(25481..27157)
                     /locus_tag="JQH51_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY6075473.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /locus_tag="JQH51_001053"
     CDS             complement(27395..28615)
                     /locus_tag="JQH51_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6075474.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /locus_tag="JQH51_001054"
     CDS             28833..30485
                     /locus_tag="JQH51_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY6075475.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQH51_001055"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQH51_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY6075476.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="JQH51_001056"
     CDS             complement(31205..31999)
                     /locus_tag="JQH51_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075477.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="JQH51_001057"
     CDS             32137..32478
                     /locus_tag="JQH51_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY6075478.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQH51_001058"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQH51_001058"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY6075479.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="JQH51_001059"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="JQH51_001059"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY6075480.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="JQH51_001060"
     CDS             36394..37686
                     /locus_tag="JQH51_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6075481.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /locus_tag="JQH51_001061"
     CDS             37811..38218
                     /locus_tag="JQH51_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY6075482.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="JQH51_001062"
     CDS             complement(38219..38677)
                     /locus_tag="JQH51_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY6075483.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="JQH51_001063"
     CDS             complement(38674..39591)
                     /locus_tag="JQH51_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY6075484.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="JQH51_001064"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="JQH51_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY6075485.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="JQH51_001065"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="JQH51_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY6075486.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQH51_001066"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQH51_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY6075487.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQH51_001067"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQH51_001067"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY6075488.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQH51_001068"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQH51_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="JQH51_001069"
     CDS             43550..44236
                     /locus_tag="JQH51_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6075489.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46512
                     /locus_tag="JQH51_001070"
     CDS             44233..46512
                     /locus_tag="JQH51_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6075490.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGGLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCDVRHAWVCFR
                     PGGRNWCGNGTGSVAGESV"
     gene            46490..46648
                     /locus_tag="JQH51_001071"
     CDS             46490..46648
                     /locus_tag="JQH51_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6075491.1"
                     /translation="MLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGK
                     ALFRQFVG"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="JQH51_001072"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="JQH51_001072"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY6075492.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="JQH51_001073"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="JQH51_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY6075493.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="JQH51_001074"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="JQH51_001074"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY6075494.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="JQH51_001075"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="JQH51_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY6075495.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="JQH51_001076"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="JQH51_001076"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY6075496.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="JQH51_001077"
     CDS             52939..>53211
                     /locus_tag="JQH51_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY6075497.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12831 a        13689 c        14016 g        12675 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaacaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DADLEE010000017        49830 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000017 DADLEE010000000
VERSION     DADLEE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.017"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1224
                     /locus_tag="JQH51_001078"
     CDS             <1..1224
                     /locus_tag="JQH51_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY6075498.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JQH51_001079"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JQH51_001079"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY6075499.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JQH51_001080"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JQH51_001080"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY6075500.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JQH51_001081"
     CDS             2714..3427
                     /locus_tag="JQH51_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY6075501.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JQH51_001082"
     CDS             complement(3399..4076)
                     /locus_tag="JQH51_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY6075502.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JQH51_001083"
     CDS             complement(4070..4747)
                     /locus_tag="JQH51_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY6075503.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JQH51_001084"
     CDS             complement(4735..5118)
                     /locus_tag="JQH51_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY6075504.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JQH51_001085"
     CDS             complement(5134..5442)
                     /locus_tag="JQH51_001085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075505.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JQH51_001086"
     CDS             complement(5443..6021)
                     /locus_tag="JQH51_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY6075506.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JQH51_001087"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="JQH51_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JQH51_001088"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JQH51_001088"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY6075507.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JQH51_001089"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JQH51_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY6075508.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JQH51_001090"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JQH51_001090"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY6075509.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JQH51_001091"
     CDS             10564..11424
                     /locus_tag="JQH51_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY6075510.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JQH51_001092"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JQH51_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY6075511.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JQH51_001093"
     CDS             12291..13031
                     /locus_tag="JQH51_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY6075512.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="JQH51_001094"
     CDS             13198..14148
                     /locus_tag="JQH51_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6075513.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JQH51_001095"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JQH51_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JQH51_001096"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JQH51_001096"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY6075514.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JQH51_001097"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JQH51_001097"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY6075515.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JQH51_001098"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JQH51_001098"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY6075516.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /locus_tag="JQH51_001099"
     CDS             complement(19724..20617)
                     /locus_tag="JQH51_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY6075517.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JQH51_001100"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JQH51_001100"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY6075518.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /locus_tag="JQH51_001101"
     CDS             21915..23147
                     /locus_tag="JQH51_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY6075519.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JQH51_001102"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JQH51_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY6075520.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JQH51_001103"
     CDS             23768..23827
                     /locus_tag="JQH51_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY6075521.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="JQH51_001104"
     CDS             complement(23928..24530)
                     /locus_tag="JQH51_001104"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY6075522.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="JQH51_001105"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JQH51_001105"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="JQH51_001106"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="JQH51_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY6075523.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="JQH51_001107"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="JQH51_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY6075524.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="JQH51_001108"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="JQH51_001108"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY6075525.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="JQH51_001109"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="JQH51_001109"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY6075526.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="JQH51_001110"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="JQH51_001110"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY6075527.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="JQH51_001111"
     CDS             complement(29676..29759)
                     /locus_tag="JQH51_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075528.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="JQH51_001112"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="JQH51_001112"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY6075529.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="JQH51_001113"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="JQH51_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY6075530.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="JQH51_001114"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="JQH51_001114"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY6075531.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="JQH51_001115"
     CDS             34816..35559
                     /locus_tag="JQH51_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6075532.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /locus_tag="JQH51_001116"
     CDS             complement(35616..37049)
                     /locus_tag="JQH51_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY6075533.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="JQH51_001117"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="JQH51_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="JQH51_001118"
     CDS             37568..38227
                     /locus_tag="JQH51_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY6075534.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="JQH51_001119"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="JQH51_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY6075535.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="JQH51_001120"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="JQH51_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY6075536.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="JQH51_001121"
     CDS             39893..40300
                     /locus_tag="JQH51_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075537.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="JQH51_001122"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="JQH51_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY6075538.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="JQH51_001123"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="JQH51_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY6075539.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="JQH51_001124"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="JQH51_001124"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY6075540.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="JQH51_001125"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="JQH51_001125"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY6075541.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="JQH51_001126"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="JQH51_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY6075542.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="JQH51_001127"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="JQH51_001127"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY6075543.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="JQH51_001128"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="JQH51_001128"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY6075544.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="JQH51_001129"
     CDS             47705..47776
                     /locus_tag="JQH51_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075545.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="JQH51_001130"
     CDS             complement(47773..47898)
                     /locus_tag="JQH51_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075546.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /locus_tag="JQH51_001131"
     CDS             complement(47900..49348)
                     /locus_tag="JQH51_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY6075547.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12451 c        13406 g        11922 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattgattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtca
//
LOCUS       DADLEE010000018        49739 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000018 DADLEE010000000
VERSION     DADLEE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49739
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.018"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..185
                     /locus_tag="JQH51_001132"
     CDS             <1..185
                     /locus_tag="JQH51_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY6075548.1"
                     /translation="SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVE
                     LYDKVIGHYLNIKHYQ"
     gene            complement(179..1357)
                     /gene="setB"
                     /locus_tag="JQH51_001133"
     CDS             complement(179..1357)
                     /gene="setB"
                     /locus_tag="JQH51_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY6075549.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1725..2855
                     /locus_tag="JQH51_001134"
     CDS             1725..2855
                     /locus_tag="JQH51_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY6075550.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2855..3793
                     /gene="fruK"
                     /locus_tag="JQH51_001135"
     CDS             2855..3793
                     /gene="fruK"
                     /locus_tag="JQH51_001135"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY6075551.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3810..5501
                     /gene="fruA"
                     /locus_tag="JQH51_001136"
     CDS             3810..5501
                     /gene="fruA"
                     /locus_tag="JQH51_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY6075552.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5925..6866
                     /locus_tag="JQH51_001137"
     CDS             5925..6866
                     /locus_tag="JQH51_001137"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY6075553.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6854..7792
                     /locus_tag="JQH51_001138"
     CDS             6854..7792
                     /locus_tag="JQH51_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY6075554.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7886..9136
                     /locus_tag="JQH51_001139"
     CDS             7886..9136
                     /locus_tag="JQH51_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY6075555.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9207..9901)
                     /gene="yeiL"
                     /locus_tag="JQH51_001140"
                     /pseudo
     CDS             complement(9207..9901)
                     /gene="yeiL"
                     /locus_tag="JQH51_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            10032..10973
                     /gene="rihB"
                     /locus_tag="JQH51_001141"
     CDS             10032..10973
                     /gene="rihB"
                     /locus_tag="JQH51_001141"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY6075556.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11073..12323
                     /locus_tag="JQH51_001142"
     CDS             11073..12323
                     /locus_tag="JQH51_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY6075557.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12379..13467)
                     /locus_tag="JQH51_001143"
     CDS             complement(12379..13467)
                     /locus_tag="JQH51_001143"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6075558.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="JQH51_001144"
     CDS             complement(13470..14327)
                     /gene="nfo"
                     /locus_tag="JQH51_001144"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY6075559.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14401..15450)
                     /locus_tag="JQH51_001145"
     CDS             complement(14401..15450)
                     /locus_tag="JQH51_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY6075560.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15549..16430
                     /locus_tag="JQH51_001146"
     CDS             15549..16430
                     /locus_tag="JQH51_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6075561.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16636..18105
                     /gene="lysP"
                     /locus_tag="JQH51_001147"
     CDS             16636..18105
                     /gene="lysP"
                     /locus_tag="JQH51_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY6075562.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18397..20388
                     /gene="cirA"
                     /locus_tag="JQH51_001148"
                     /pseudo
     CDS             18397..20388
                     /gene="cirA"
                     /locus_tag="JQH51_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="JQH51_001149"
                     /pseudo
     CDS             complement(20420..21255)
                     /gene="yeiG"
                     /locus_tag="JQH51_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21513..22181
                     /gene="folE"
                     /locus_tag="JQH51_001150"
     CDS             21513..22181
                     /gene="folE"
                     /locus_tag="JQH51_001150"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY6075563.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22198..23274
                     /locus_tag="JQH51_001151"
     CDS             22198..23274
                     /locus_tag="JQH51_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY6075564.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23416..24456
                     /gene="galS"
                     /locus_tag="JQH51_001152"
     CDS             23416..24456
                     /gene="galS"
                     /locus_tag="JQH51_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY6075565.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24736..25734
                     /gene="mglB"
                     /locus_tag="JQH51_001153"
     CDS             24736..25734
                     /gene="mglB"
                     /locus_tag="JQH51_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY6075566.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25795..27315
                     /gene="mglA"
                     /locus_tag="JQH51_001154"
     CDS             25795..27315
                     /gene="mglA"
                     /locus_tag="JQH51_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY6075567.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27331..28341
                     /gene="mglC"
                     /locus_tag="JQH51_001155"
     CDS             27331..28341
                     /gene="mglC"
                     /locus_tag="JQH51_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY6075568.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28377..29590)
                     /gene="preA"
                     /locus_tag="JQH51_001156"
                     /pseudo
     CDS             complement(28377..29590)
                     /gene="preA"
                     /locus_tag="JQH51_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29584..30822)
                     /locus_tag="JQH51_001157"
     CDS             complement(29584..30822)
                     /locus_tag="JQH51_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY6075569.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(31016..31255)
                     /locus_tag="JQH51_001158"
     CDS             complement(31016..31255)
                     /locus_tag="JQH51_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY6075570.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="JQH51_001159"
     CDS             complement(31258..31977)
                     /gene="sanA"
                     /locus_tag="JQH51_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY6075571.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="JQH51_001160"
     CDS             complement(32127..33011)
                     /gene="cdd"
                     /locus_tag="JQH51_001160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY6075572.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33141..33836)
                     /locus_tag="JQH51_001161"
     CDS             complement(33141..33836)
                     /locus_tag="JQH51_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY6075573.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33833..34231)
                     /locus_tag="JQH51_001162"
     CDS             complement(33833..34231)
                     /locus_tag="JQH51_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY6075574.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34470..35417
                     /gene="dusC"
                     /locus_tag="JQH51_001163"
     CDS             34470..35417
                     /gene="dusC"
                     /locus_tag="JQH51_001163"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY6075575.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="JQH51_001164"
     CDS             complement(35788..35871)
                     /gene="yohP"
                     /locus_tag="JQH51_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY6075576.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36005..37531
                     /gene="mdtQ"
                     /locus_tag="JQH51_001165"
                     /pseudo
     CDS             36005..37531
                     /gene="mdtQ"
                     /locus_tag="JQH51_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37584..38345
                     /locus_tag="JQH51_001166"
                     /pseudo
     CDS             37584..38345
                     /locus_tag="JQH51_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38462..39040)
                     /locus_tag="JQH51_001167"
     CDS             complement(38462..39040)
                     /locus_tag="JQH51_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6075577.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39210..39797
                     /locus_tag="JQH51_001168"
     CDS             39210..39797
                     /locus_tag="JQH51_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY6075578.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39971..40903
                     /gene="pbpG"
                     /locus_tag="JQH51_001169"
     CDS             39971..40903
                     /gene="pbpG"
                     /locus_tag="JQH51_001169"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY6075579.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40941..42656)
                     /gene="dld"
                     /locus_tag="JQH51_001170"
     CDS             complement(40941..42656)
                     /gene="dld"
                     /locus_tag="JQH51_001170"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY6075580.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42852..45149
                     /gene="bglX"
                     /locus_tag="JQH51_001171"
     CDS             42852..45149
                     /gene="bglX"
                     /locus_tag="JQH51_001171"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY6075581.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45401..46318
                     /gene="osmF"
                     /locus_tag="JQH51_001172"
     CDS             45401..46318
                     /gene="osmF"
                     /locus_tag="JQH51_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY6075582.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46325..47482
                     /gene="yehY"
                     /locus_tag="JQH51_001173"
     CDS             46325..47482
                     /gene="yehY"
                     /locus_tag="JQH51_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY6075583.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47475..48401
                     /gene="yehX"
                     /locus_tag="JQH51_001174"
     CDS             47475..48401
                     /gene="yehX"
                     /locus_tag="JQH51_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY6075584.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48406..49137
                     /gene="yehW"
                     /locus_tag="JQH51_001175"
     CDS             48406..49137
                     /gene="yehW"
                     /locus_tag="JQH51_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY6075585.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49118..49225)
                     /locus_tag="JQH51_001176"
     CDS             complement(49118..49225)
                     /locus_tag="JQH51_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY6075586.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49285..49739)
                     /locus_tag="JQH51_001177"
                     /pseudo
     CDS             complement(49285..49739)
                     /locus_tag="JQH51_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT        12023 a        12251 c        13360 g        12105 t
ORIGIN      
        1 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgaac
       61 gacataatct gaatctgaga caacatctgg caaggctggg acggaagtca ctgtcgttct
      121 caaaatcggt ggagctgtat gacaaggtca tcgggcatta tctgaacata aaacactatc
      181 agtaagttgg agtcattacc caaaacagag tggcgataat catcaccatc gcaaaccaga
      241 acacagcgtg ataattccag atctcggcga cgatgcccgc cactgatcct gcgatgatcc
      301 agcccacgcg cgacgtgttg gtatagagcg tggtggctga acctgcctga ccgggcatca
      361 aatcctgaaa atagagcatc ccgatgccgc ccagaatgcc aataaaaata gcatttagca
      421 gctgcaagcc caacagaatg gcaggtgaat gcgccatcag cattcctgcg taaaaacaga
      481 cgccacccac ggcagcaacg cgcattaaga aacgcttacc cagacgtttg gcgaaatatc
      541 cggcaatcaa catcgtcggg atttccagcc cggcggcggt ccccatcatc acaccggcca
      601 gtttctcggg aagatgcagt tcgttgataa taaatagcgg catgttgatg atgtacaggc
      661 tgttcgagcc ccacatcaat gtacaaatga caaacagcag tagcgtatcg cgacggttac
      721 ggcgcggcgc ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc gacggtaaaa
      781 acagccacac catcacgccg caaacaataa acgctaccgc tgcgctcaga tacattaccg
      841 taaagctgaa acccatcgct aacgcataag ccagcggtgg gccaatgacc catgccagtg
      901 aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat
      961 gttcacgggc aagggcaaac atttgcgggt tagcggtcga gccaaagctg ctaagaaaga
     1021 cgccaacgaa tagcaaaaca aagtagttgc gattccaagc aaaaagggtg caggccagca
     1081 cgcctaacag gcagcaaaag acaatcagcg atttgcgatc gccgcgctta tcagagcgcc
     1141 cagcgagaaa ctgacttacc agaatcccaa tgacagcgct gccggtaaag aagaatccca
     1201 ccatcgccgg acgggcatgt acttcatcgg taaggaaaat actgagtgtc ggggtttgca
     1261 gagcacccgc aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa
     1321 acgatttcgc gctggagact gcgggggagt tatgcatgct ggtcgatcac tggaagagga
     1381 agacgcggag tttacgctgc ttgtcagaaa ataaacagac agcgaaatca aaagcgccac
     1441 gaataatcaa aatgccattt cactgttgct gattggctgc tgacgctgct gaaatgaaat
     1501 tgctgataac ggattttccc atcagcaatt aggaaaaatg acaaaaaatt gtgcagcaca
     1561 tcaaactttt gctcataact ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct
     1621 ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa gctaggttgg cgcttttttt
     1681 ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc cagttatccg
     1741 tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg
     1801 ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc
     1861 gcgaacagca aacctcaacg ttcctcggca atggtattgc tattccacac ggcactaccg
     1921 acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca
     1981 cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc tcggataaac
     2041 atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt gctgaacaac
     2101 tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa aagcagagtg
     2161 agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc gatctgctga
     2221 ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag ggcagttgac gccactttcg
     2281 tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg ctgagcgata
     2341 gcgccgaagg caatctgcgt agtgcgattg cggtaagccg tgcggcaaat gcttttgatg
     2401 tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat cagcccatcg
     2461 cggttcttaa gcgtctcgct gatttgttgc tcgacaataa agctgaccgc ttgctgaaag
     2521 cggatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt
     2581 taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc
     2641 tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt gatggcaccg
     2701 gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt aagaaaggtc
     2761 atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa gccattggcg
     2821 acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg ctacgatcac
     2881 ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa
     2941 cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt
     3001 aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca atcaggatgg
     3061 ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg tacaggggcg
     3121 cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact tcaacttctc
     3181 gggtttcgaa gtcacccccg ccgactggga acgctttgtg actgattctc tgagctggct
     3241 cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc
     3301 gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct ttgatagtag
     3361 ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg
     3421 cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc
     3481 acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc
     3541 gctttgggtt aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg atgtcgtaag
     3601 caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc tgatgcgtga
     3661 atccagtgaa cacacactgc gtctggcgac agctgttgca gccctggcgg taagtcaaag
     3721 caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca
     3781 accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt gacgctaatc
     3841 tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa
     3901 aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc ggtgattcca
     3961 tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt tcccgggcag
     4021 ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc
     4081 cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg
     4141 cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa accgaagcga
     4201 aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa
     4261 tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat atcgaagtgg
     4321 atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg gcgctgaaga
     4381 aaacctcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca
     4441 aagctcaaac ggcgaccact gaaggtaaga aagagagtgc aggcgcttat cgtcacttgc
     4501 taacgggcgt ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt atcgcgcttt
     4561 cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg gcgctgatgc
     4621 agattggtgg tggttcagcc tttgcgctga tggtgccggt actggcaggt tatattgcct
     4681 tctccattgc cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg ctggcggtca
     4741 gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt tacattgcga
     4801 agttaatcag tacgcaactg aaactgccgc agagtatgga ggcgctgaaa ccgatcctga
     4861 tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg atcggtaaac
     4921 cagttgctgg cattctcgaa gggctgactc actggctgca gaccatgggg actgcgaatg
     4981 cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa
     5041 acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg
     5101 cggcgattat ggcggcaggt atggtgccac cgctggcaat gggcctggca acaatggtgg
     5161 cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg gtattgggac
     5221 tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc
     5281 cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac
     5341 tgatggcacc gcacggtggt ctgtttgttc tgctgatccc tggcgctatt acgccggtat
     5401 tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc tatgccttcc
     5461 tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg tgttacaggg
     5521 cagaaatttc ctgccctgta acatttgatg agcgcaaaga gaatgaccag cttgtgaatg
     5581 taattttatg aatttaaatg gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca
     5641 ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttagtgtt cctgttctgt
     5701 gcatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat tttgtgagcc
     5761 tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa aacatgcata
     5821 ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt atctatattc
     5881 ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc gaaaaggatt
     5941 atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat gaatcattaa
     6001 attatgcgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta gggcgcaata
     6061 ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc gtaggcagtg
     6121 atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat gtcgataaat
     6181 gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat aataccggtg
     6241 aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct gaatatctcg
     6301 cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcggactgt aatatcagtg
     6361 aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt gtcgatccgg
     6421 tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa tcagaaccac actctgaagc
     6481 caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt gacgatgtgg
     6541 caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg agcatgggcg
     6601 gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg ccgatcaaaa
     6661 ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt
     6721 gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg
     6781 cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt atatcgttag
     6841 tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa tttctccgga
     6901 agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga ccattatttc
     6961 tcacgggatg ccgttcccac aaaatgccca gaccgcaatt gaagtagaag aaactattcg
     7021 taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga aagtgggttt
     7081 aagcaaagaa aaaattgaat tactgggtcg tgaagggcat aacgtgacca aagttagtcg
     7141 tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac
     7201 gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa ttggtggtgt
     7261 gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac tggcaaatac
     7321 taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat taaccactga
     7381 gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc tgcctgcgtt
     7441 tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc
     7501 ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc
     7561 gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag atcaagccgt
     7621 cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc tgctggcgcg
     7681 cgttgctgag ctgaccagcg gtgacagcct gaaatccaat atccagctgg tgttcaacaa
     7741 cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac
     7801 gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct gacgccgggt
     7861 ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg ggatggtggt
     7921 gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt
     7981 tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact tcccaccggg
     8041 aaaatgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt acagtgatgc
     8101 cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg
     8161 tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta ctgcgctcat
     8221 cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg gcagcatttt
     8281 ccagaaagcc ctcaacatca gcaaaatcga atcttttgtt gcggttacca ctattttcct
     8341 cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcgtga atcgcaacga
     8401 gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcggtga tgattggtta
     8461 tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg cgatccccgg
     8521 cggtattttg tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg tcacatttga
     8581 aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc
     8641 gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat
     8701 tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt tcgccaatgc
     8761 ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga
     8821 ctggagtgat gccaaccttg ctggtagcct gattgggcag aaactggcga ttaacgaatt
     8881 cgtcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac
     8941 cgtggcgatt atatcctttg cgctttgtgg ttttgctaac tttggttcta tcggtgttgt
     9001 cgttggcgca ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc agcttggttt
     9061 acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgactattg ccgggttctt
     9121 tattggtctg gcgtaatgct cgatttgtag accggataaa acgtattaag cgttgcatcc
     9181 ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt attctccggg
     9241 tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata tcctttcttt
     9301 taattaataa accgtcgtga atgaattgcg cgagaacata taaaagatgt cgataagaaa
     9361 cgcctaaata ctctgccgct tgcgtatgct tttcgtgata aagatcacct tcttgtgaga
     9421 gtaatataaa tgctgccagg cgattcacta acggaaatga ttgattctga gttaaagaaa
     9481 caatattacg ataattttta tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt
     9541 cgtttaataa cagcggacgg taatgtttca tagggagcgc aaggcaccaa cactcttcaa
     9601 tagcctgcac cgcacgcggt tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac
     9661 agggagcggc aaagaaatcg atcagcgaca cgcgaccatt ggcaagcgtg gcgtaaagcc
     9721 tggcgcggcc tcgcgtcaga taaaacagcc atgacggctg ttgcccttcc tgcacaatgt
     9781 agtcacgcgc taaaaaatga aacagccgcg tatcggctga aacatccgtt gaaaagcaat
     9841 ccttaaacgc agattcactc atcaattgct gcttaaggtc attattgtgg atttctttca
     9901 cgatgtttat ccgctatgag atttctcata tttagcatac gtacgctgtt gttaagattc
     9961 aatcattggc ccggtcgaat tttctttgct gtagaaaaaa ataacgcaac tggaaacaga
    10021 ggaaataaac aatggaaaag agaaaaatta ttctggattg tgagccgggt catgatgatg
    10081 ctattgcaat gatgatggca gcgaaacatc cggcaataga tttattaggc atcactattg
    10141 tagcgggtaa tcagacgctt gataaaacat taattaatgg cctgaatgtt tgccagaaac
    10201 tggagattaa tgttccggtt tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg
    10261 ttgccgataa tattcacggt gaaaccggac tggatggccc ggtattcgag ccgctgaccc
    10321 gccaggcaga aagtacccat gcggtgaaat atatcatcga taccctgatg gcaagcgatg
    10381 gcgatatcac tctggtgccg gttggtccgc tttcaaatat cgcggtggca atgcgtatgc
    10441 aacccgcaat cctgcccaaa atccgtgaaa ttgtgctaat gggcggcgct tacggtacag
    10501 gcaacttcac cccatctgcc gagttcaata tctttgccga cccggaagcc gcccgcgtgg
    10561 tgttcacctc cggcgttcca ttagtgatga tgggcctcga tcttaccaac cagaccgttt
    10621 gcaccccgga tgtaattgct cggatggaaa gggtaggcgg ccctgccgga gagctgttca
    10681 gcgacatcat gaacttcact ctcaaaacgc agttcgaata ctacggcctg gctggcggcc
    10741 cggtgcatga cgccacctgc attggttatc tgatcaaccc tgatggcatt aaaacccagg
    10801 atatgtacgt cgaagtggac gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg
    10861 aactgggcgt tcttggcaag cccgctaata ccaaagtcgg catcactatt gataccgact
    10921 ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat caaaacccat taagtgttga
    10981 taacaagccg ggacagtatc gcaagatgca ggcccggcta tttctgatgg gcagaaagcc
    11041 gtcgcccaaa atgacatgaa gagataatca ctatggatgt catgagaagt gttctgggaa
    11101 tggtggtact gctggcaatt gcgtttttac tgtcagtaaa caagaagaag atcagcctgc
    11161 gtaccgttgg cgcggcgtta gtgttacagg tcgtgattgg cgggattatg ctttggttac
    11221 cgccagggcg ttgggtcgca gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca
    11281 gcgacgcagg tagcgcattt atcttcggtt ctctggtcgg accgaaaatg gataccttat
    11341 ttgatggcgc aggatttatc tttggtttca gggtattacc ggcaattatc ttcgtcactg
    11401 cactggtgag tattctctac tacatcggtg tgatggggat cttaattcgc attctcggcg
    11461 gtatcttcca gaaagcttta aatatcagca agatcgagtc attcgtcgcg gtcaccacca
    11521 ttttcctcgg gcaaaacgaa attccggcga tcgtcaaacc ctttatcgat cgtctgaatc
    11581 gcaatgaatt atttacagcg atttgtagtg gcatggcctc gattgctggt tcgacaatga
    11641 ttggttacgc cgcactgggc gtacctgtgg aatatttgct ggcggcatcg ttaatggcga
    11701 tccctggcgg gatcttgttt gcccgcctgt taagcccggc tacggaatct tcgcaggttt
    11761 cttttaataa cctctctttc accgaaacac cgccaaaaag cattattgaa gccgatgcga
    11821 caggggcaat gaccgggctg aaaatcgccg ccggtgtagc gacagttgtt atggcatttg
    11881 tcgccatcat tgcgttaatt aacggtatta tcggcggcgt tggcggctgg tttggttttg
    11941 aacatgcctc gctggagtcc attttaggtt acctgctggc cccacttgcg tgggtaatgg
    12001 gtgttgactg gagtgatgca aatcttgccg ggagtttgat tggacagaaa ctggcaataa
    12061 atgaatttgt cgcttatctc aatttctcac cctatctgca aacggctggc actctggatg
    12121 ctaaaaccgt ggcgattatt tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg
    12181 gggtggtggt gggggcgttt tctgcggttg cgccacaccg tgcgccggaa atcgcccagc
    12241 ttggtttacg cgcgctggcg gcggcgacac tttctaatct gatgagtgcc accattgccg
    12301 ggttctttat tggtttagcg tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg
    12361 tcgaatccgg caacattgtt acaccattgg cactaatgac agcgcgttat cggcagacag
    12421 ggtagggttg tttaagctgc cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg
    12481 ggcgctgtca cggaaattgt ttccctccag aaagctatat accagacccg ccataaaacc
    12541 atcgtcagca ccaaaactgt ctaccgtcgt atgcgctggc acagtcagca aaaattgttc
    12601 accatccttt tcgctgcaat aaactgactc atcgggcaaa taaacaaaca gttgctgaac
    12661 accttgctga tgcaatgcat tcactgcggc attacggtca gcatcgctgg tgatcgcctg
    12721 tccccataaa atttccagct ccggtaaagt gggtttcagg gtgtgaatat gcgccagcca
    12781 gtgtttgatt ttgcccgctt tgaattctga aacggtatcg acaaacaccg ggatctcatc
    12841 agcgagggta aagacccatt ccagcgcctc ggctgtcagg ttgcaatcag ccagtaccac
    12901 gcccgcatga cgaagtaaat cgcgcgaccc gttcagtagt tgcggtgtca actgttccag
    12961 cagatgggta tcgttaattg ccagcacggt ttgatcgtct cgattggcaa ttgccagata
    13021 cgtcgatgtg ctttgaccat gcaaacgaac gcagccggag acattcacgc cggcacggcg
    13081 cgtttcttcc aggagcattt cgccataaaa gtcatcgcca atcactgaaa gcaaatggac
    13141 gtcacggcct aacagcgcca gattgtgggc gatattgcgt ccaacgccgc ctgccgaaca
    13201 atgaattgta ccgggatgag aagccgcttg cgggtaatgg atatccgcca tcccgcgaat
    13261 atccatattg attgtcccca ccactacgca gtattcctgc tcggtgagaa tgtaaccttt
    13321 gtctttaatc cggcctttgc gcattaaatc cataatatgc gccgcaacgc gcgaacgact
    13381 gatttgcagc atgtccgcaa tttcgttctg ctgaatcagc gggttacgcc gtaaaattgc
    13441 aaggatctcc ttttcccggt tatttatctt caggctaccg ctttttcagt ttgttgcgct
    13501 ttcagccagg cgatctcttc tgcccagata tccgggttga tggtttcaag gatcagcgga
    13561 atgccgtcga aacggtcgtt ctgcataatc cagcggaacg catcatgacc gatattgcct
    13621 tcaccgaggc tatgatggcg gtcaacgcgg ctgccaaagg tacttttcgc atcgttaagg
    13681 tgcatcccgc gcaaatactt aaagccgaca atacgggcaa aatcagcgaa cgttttttcg
    13741 cattcggctg gagtacgcaa atcatacccg gcagcgaaag catggcaggt atcaatgcag
    13801 acgccgacgc gggatttatc ttccacgccg tcgataatcg ccgcgagatg ttcgaattta
    13861 aaccctaagt tactgccctg accggcggtg ttttcaatca ccgctgttac accttgagtt
    13921 ttatccagcg caatgttgat ggattcggca atacgcgcaa ggcaatcctc ttctgaaatc
    13981 tgcatcagat ggctgccagg gtggaagttg agcaaagaaa gccccagttg ttcgcaacgc
    14041 tgcatttcat ctataaaggc atcgcgcgat ttttccagag cttcagtgac cggatgtccg
    14101 aggttgatca gataactgtc gtggggaaga atttgcgccg atgtgtagtg atatttttca
    14161 caggcggctt tgaattcatc gatggtttgc gtcgtgagcg gtgcggcacg ccactgacgt
    14221 tggtttttgg tgaacaaggc aaacgcggtt gcgtcgattt cggcggcgcg aattgcggca
    14281 tttgccagac cgccagcagc actaacgtgc gctccaatgt atttcatgcg aggactcctg
    14341 ttaaacccgc tggaggaaaa cggtaatgat agcgggttaa caggagcaag aagtagtggt
    14401 ttatgcgatg acgctttgaa tcacatagtt aatcgcacca ccaccaacaa tcagccaggc
    14461 aaacagtacc agtgccatca gcagaggttt cgccccagct tttttcagcg cgctgacgtg
    14521 agtggtcaga cccagcgccg ccatcgccat tgccagcagg aaggtatcca gcgttaccag
    14581 catgttcacc acgctctgcg gtaacaggtg gaacgagtta aagatggcaa ctacgatgaa
    14641 caagatggca aaccacggaa tagtgatttt gcttttctcg ccgctgttcg ccccagacag
    14701 ctgtttaaca cgcgccgcca gcaggatgag gaacggagcc agcatcatca cgcgcagcat
    14761 tttggaaata actgctgcgt tttctgcatc cgggctgatg gcatgacctg ccgccaccac
    14821 ctgcgccact tcgtgcacag tagaaccaat gtagataccg aaagtttccg gactaaacca
    14881 ttgagacatc agcggatata tcgccgggta gaggaaaatc gcgacggtcc cgaagataac
    14941 aacggttgca acagccacgg ttactttact ggcttccgct ttcactaccg gctcagtcgc
    15001 cagtaccgcg gcagcaccac agatactgct accggcaccg atcaaccaac tggtgtgctt
    15061 atccagacca aacactttct gccccaggaa gcaagccagc aggaaggtac tggacagcgt
    15121 cagcacgtca atgatgatcc cactgatacc gacatcggca atttgcgaga acgtcagacg
    15181 gaagccataa agaatgatac ccagacgtaa taaatattgc ttggcaaaca gcacaccacc
    15241 gtcacagctt ttccagatgt gcggatagat ggtgttgcct aaaaccatcc ccaacaagat
    15301 tgcgagggtg agggcactaa acccggcacc cgcaaccgcg ggaatggaac caccccacag
    15361 ggcgaccccg gtgataactg cactcagggc taaccccgga ataaaatgcc acagtgtacg
    15421 atgttgtttc tgtaaggtga tattcgtcat aaccctctcc tttaaccggg cataaggtta
    15481 cgactaactg gtttaaaaat aaaattgatt atatatttat aattaatctt tataagtggt
    15541 aagcgactat gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg
    15601 gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct
    15661 tgactgacct ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg
    15721 ttgttaatga acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg
    15781 ttgaaatcga acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta
    15841 ccatcggtaa ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt
    15901 tgccgattga acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc
    15961 gcgttgatat tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt
    16021 ggctggaaga cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg
    16081 tcaccttaga acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc
    16141 gggatattgt cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat
    16201 taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt
    16261 cgcgacgtgt gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc
    16321 tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg
    16381 cgctacggcg ctttctggac tattgcgatc ccgcaaatgt gccgcgttaa gttgctgtac
    16441 aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc cttcagcatg cattcgccag
    16501 aaaagagatt ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg
    16561 tgtatttctg ccggaaggat tgccaatcgt ctgctacaat cgcgccatca tttttaagat
    16621 ggatagcatt tttgtatggg ttccgaaact aaaactacag aagcgccggg cttacgccgt
    16681 gaattaaagg cgcgtcacct gacgatgatt gccattggcg gttccatcgg tacaggtctt
    16741 tttgttgcct ctggcgcaac gatttctcag gcaggtccgg gcggggcatt gctctcgtat
    16801 atgctgattg gcctgatggt ttacttcctg atgaccagtc tcggtgaatt ggctgcatat
    16861 atgccggttt ccggttcgtt tgccacttac ggtcagaact atgttgaaga aggctttggc
    16921 ttcgcgctgg gctggaacta ctggtacaac tgggcggtga ctattgccgt tgacctggtt
    16981 gcagcacagc tggtcatgag ctggtggttc ccggatacac cgggctggat ctggagtgcg
    17041 ttgttcctcg gcgttatctt cctgttgaac tacatctctg ttcgcggctt tggtgaagcg
    17101 gaatactggt tctcactgat caaagtcacg acagttattg tctttatcat cgttggtgtg
    17161 ctgatgatta tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa ctggacaatc
    17221 ggcgaagcgc cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat gattgtcggc
    17281 ttctctttcc agggaaccga gctgatcggt attgctgcag gcgagtccga agatccggcg
    17341 aaaaacattc cacgcgcggt acgtcaggta ttctggcgaa tcctgttgtt ctatgtgttc
    17401 gcgatcctga ttatcagcct gattattccg tataccgatc cgagcctgct gcgtaacgat
    17461 gttaaagaca tcagcgtcag tccgttcacc ctggtgttcc agcacgcggg tctgctctct
    17521 gcggcggcgg tgatgaacgc agttattctg acggcggtgc tgtcagcagg taactccggt
    17581 atgtatgcgt ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc gccgcgcatt
    17641 ttcgctaaat tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac gacggtgatt
    17701 gccggtttgt gcttcctgac ctccatgttt ggcaaccaga cggtatacct gtggctgctg
    17761 aacacctccg ggatgacggg ttttatcgcc tggctgggga ttgccattag ccactatcgc
    17821 ttccgtcgcg gttacgtatt gcagggacac gacattaacg atctgccgta ccgttcaggt
    17881 ttcttcccac tggggccgat cttcgcattc attctgtgtc tgattatcac tttgggccag
    17941 aactacgaag cgttcctgaa agataccatt gactggggcg gcgtagcggc aacgtatatt
    18001 ggtatcccgc tgttcctgat tatttggttc ggctacaagc tgattaaagg aactcacttc
    18061 gtacgctaca gcgaaatgaa gttcccgcag aacgataaga aataagtttc ctcccttcct
    18121 tgctaagccc tctcaaccga gagggctttt tcaattccat ttccctgaca aatcatgcgg
    18181 atataaaatt taacatttgg attgataatt gttatcgttt gcattatcgt tacgacgcaa
    18241 tcaaaaaagg ctgacaaatc agaggctgtt ccggctttct gggatgatca cctgcataca
    18301 aataagtcca ccgcgatgct gccgtacgca aggggacgta aagaagatgt gagcgatacc
    18361 cattttattt tcgtagttac ctcatggaga tatggaatgt ttaggttgaa ccctttcgta
    18421 cgggtcgggc tgtgtttgtc cgctatttct tgtgcatggc ctgtgttagc ggtcgatgat
    18481 gatggcgaaa cgatggttgt cactgcatct tccgtggaac aaaatcttaa agatgcaccc
    18541 gccagtatca gcgtcattac ccaggaagac ctgcagcgaa aaccggtaca gaatctgaag
    18601 gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg aaggggataa ccgtaagggc
    18661 gttagtattc gtggtctgga cagcagctac accctgattc ttgtcgacgg taaacgcgtt
    18721 aactcccgca atgccgtctt ccgccacaat gatttcgatc tgaactggat cccggtcgat
    18781 tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt cgctatacgg ctccgatgcg
    18841 ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc agaaatggtc gggtaccgtt
    18901 accgtcgata ccaccattca ggaacatcgc gatcgcggtg atacctataa cggtcagttc
    18961 tttaccagtg gaccattaat tgatggtgtg ctgggaatga aagcttacgg cagcctggca
    19021 aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg ataccggaga aacgccgcgt
    19081 attgaaggat tctccagccg cgacggcaat gtcgaatttg cctagacacc gaatcaaaat
    19141 cacgatttta ctgccggata cggtttcgac cgtcaggatc gtgattccga ttcgctggac
    19201 aaaaaccgcc tggaacgcca gaactactgc gtcagccata atgggcgttg ggattacggc
    19261 accagcgaac tgaaatccta cggtgagaaa gtcgagaaca aaaaccctgg caacagcagc
    19321 ccgataactt ccgaaagcaa tacggtcgac ggcaaataca cgttgccgct gacggcgatt
    19381 aatcagtttc tcacggttgg cggtgaatgg cgtcacgaca aacttagcga tgcggtgaac
    19441 ctgaccgggg gaaccagctc caaaacgtct gccagccagt acgcgctgtt tgtggaagat
    19501 gaatggcgga tcttcgagcc gctggcgctg acgaccggcg tgcgtatgga cgatcacgaa
    19561 acctacggtg aacactggag tccgcgtgcc tacatggttt ataacgccac cgacaccgta
    19621 acggtgaaag ggggctgggc gacggcattt aaagcacctt ctctgttgca acttagccct
    19681 gactggacga gcaattcctg ccgtggcgca tgtaagattg tgggtagccc ggatctgaaa
    19741 ccagaaacca gcgaaagttg ggagctgggg ctttactaca tgggtgaaga aggctggctg
    19801 gaagggggtg aatccagcgt tatcgttttc cgtaacgatg tgaaagatcg tatcagcatc
    19861 agccgtacgt ctgacgtcaa cgctgcaccg ggctaccaaa actttgttgg ttttgagacg
    19921 ggcgctaacg gacggcgcat accggtattt agctactaca gcgttaacaa agctcgtatt
    19981 cagggcgtgg aaaccgaact gaaaattccg ttcaacgatg aatggaaact gtcgatcaac
    20041 tacacctaca acgatggtcg tgatgtcagc aacggcgaaa acaaaccgct atccgatctg
    20101 ccgttccata ctgctaacgg tacgctggac tggaaaccgc tggcgctgga agactggtca
    20161 ttctatgttt ctggtcacta taccgggcag aaacgcgccg acagcgcgac ggctaaaaca
    20221 ccgggcggtt ataccatctg gaataccggc gcggcctggc aggtgactaa agacgtcaaa
    20281 ctgcgcgcag gcgtgctgaa ccttggcgac aaggatctca gtcgtgacga ctacagctat
    20341 aacgaagacg gacgtcgtta ctttatggca gtggattatc gcttctgatg agaagatgcc
    20401 cggcgaaccg ggcggacttt cacttcagta aatactgcgc atggaagcgc aggtgatcct
    20461 ctataaaaga ggcgatgaag tagtaactgt gatcatatcc cggctgaata cgcagcgtca
    20521 tcggccacgc tttctggcgc gcggcttctg ccaataccgc aggttgcaac tggtcggcaa
    20581 gaaactgatc attatcgccc tgatcgataa gcgtcgggat cgcatcctgc gcgttactgg
    20641 catacatcag cgcgcaacta tcccattcca gcatgcattt ttgtcctcac ctaaataggt
    20701 gctaaacgct ttgattcccc acgggacgct gcacggattc acaatgggcg caaaggccga
    20761 aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat
    20821 tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
    20881 taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
    20941 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
    21001 gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttccgccg ctacccgctg
    21061 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
    21121 cactggtggc ggggtgtgat cacgaggggg ggggagaaag atactgaacg tcatcgggca
    21181 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
    21241 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
    21301 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
    21361 tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
    21421 ccgcctcacg cacaataatc aagctgtaaa tcagcttaat aactttgccc ccacgcaggg
    21481 tggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
    21541 tggttcatga agcgttagtt gcgagaggac tggaaacacc gctgcgcccg cccgtgcatg
    21601 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
    21661 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
    21721 tgtatgtcga tgaaattttc tccggtctgg attacgccaa tttcccgaaa atcaccctca
    21781 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
    21841 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
    21901 attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc
    21961 aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca
    22021 ataacgtggc tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg
    22081 atgcaaccag tgccacgaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
    22141 cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg attaaaaggc aggaaccatg
    22201 gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta
    22261 aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg gtacgatgct
    22321 attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc
    22381 ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc ccgtggcaga
    22441 cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg gctttattca cggtttattg
    22501 ttctgggacg gcgatattct gctggcttac gggctggtgg ggcttggcgt tttgctgtta
    22561 ttggggctga tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt
    22621 ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat
    22681 ggtgttggca acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg
    22741 atgctcattg gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt
    22801 cactatcgtc gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc
    22861 atcgccctgc aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg
    22921 ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc
    22981 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    23041 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    23101 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    23161 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    23221 cgtcagttaa ctttgcgtgc tgccggaccg gcaatatcta aaacatcaag ataacgatct
    23281 ggattacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    23341 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    23401 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    23461 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    23521 gtaatgaaag ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca
    23581 actcaggtta gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
    23641 gcgctggtaa aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc
    23701 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
    23761 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg
    23821 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    23881 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    23941 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
    24001 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    24061 actggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    24121 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    24181 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt
    24241 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
    24301 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
    24361 agccactgtt ttatgccgac gttagtgcgt cgccattctg tcgcaacgcg ccagaatgcg
    24421 gcggcgatca ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt
    24481 cacagtatct taacaatgtg atagctatga ttgcaccgtt ttaatgttgt aacccgtatg
    24541 taacagtgaa taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt
    24601 tctggagcgt taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta
    24661 acacttcatt aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga
    24721 aaaaccggag ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg
    24781 ttattcggtg ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac
    24841 gacgataact ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca
    24901 gatgttcagc tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc
    24961 gacgtattgc tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct
    25021 gcgggtacgg tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa
    25081 gaaccgtctc gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgactcc
    25141 aaagagtccg gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt
    25201 tgggatctga acaaagacgg tcagattcag ttcgtactgc tgaaaggtga accgggccat
    25261 ccggatgcag aagcacgtac cacttacgtg attaaagaat tgaacgataa aggcatcaaa
    25321 actgaacagt tacagttaga taccgcaatg tgggacaccg ctcaggcgaa agataagatg
    25381 gacgcctggc tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat
    25441 gcgatggcaa tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg
    25501 tttggcgtcg atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc
    25561 acagtactga acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg
    25621 gccgatggca aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc
    25681 gtaccttatg ttggcgtaga taaagacaac ttggctgaat tcagcaagaa ataagactga
    25741 ttgggcgcag tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc
    25801 agctcaacga ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc
    25861 tttcctggtg ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat
    25921 gcattaatgg gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt
    25981 tatcaaaaag actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc
    26041 aaagaggcac tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa
    26101 cgttcggtga tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat
    26161 caggacaaaa tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat
    26221 ccgcgtgcgc gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa
    26281 gcgttttcct ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa
    26341 aaagaggtca atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt
    26401 gtttatatct cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg
    26461 cgcgacggtc agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc
    26521 gccatgatgg ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc
    26581 gaagtcatcc tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc
    26641 tcgtttgatc tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt
    26701 accgatattg ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg
    26761 cacggcaaac agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg
    26821 gtaactgagg agcgccgctc aacgggaatt tatgcctatc tggatattgg ttttaactcg
    26881 ttaatttcca atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg
    26941 aaaagcgata cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg
    27001 caaattggtt cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta
    27061 acgcaaccag aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag
    27121 tttgaaattt atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc
    27181 tcctctgaaa tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt
    27241 ctcgtttccg gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg
    27301 tctttgcacc tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt
    27361 acttacctga aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt
    27421 ttccaggacc caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg
    27481 gtgcgtatta ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt
    27541 tctgctggtc gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg
    27601 gataacgcca acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg
    27661 attgtctgtg ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc
    27721 aacgtgacgc cgttcattac cacgctcggc acgatgatca tcgtctatgg catcaactcg
    27781 ctctattacg actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc
    27841 tttgctcagg gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg
    27901 ttgattgcgg tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt
    27961 tttgccattg gcggtaaccc ggaagcggca aaaatatctg gtgtcaacgt cggcctgaac
    28021 ctgctgatga tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc
    28081 ggacgtatcg gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg
    28141 gcgtgcgtgg taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg
    28201 accggggtaa ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca
    28261 tactggcagt acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg
    28321 aaatacgcgc gtaagaagtg atgtgattgc ggatattgcc ggatgcgtct ctggttttac
    28381 aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa
    28441 cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc
    28501 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    28561 taacagcgtc cacagccaac acatttgtca agattgatac ggggatagac aatataactg
    28621 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac
    28681 gaatcaaatc cctgatcggc gagataatgc gataacccgc tcgccatatc ttctacgatg
    28741 cgatagccgt actgcataat gccggtggtg acctgtaacg ttgctgcgcc gagcaataaa
    28801 aactccgccg catcttccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc
    28861 agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt taccgcttta
    28921 ccggaatatc cggaaatact cgatttaccg ttaacgatcg gcataccgat tttctgatta
    28981 agatcgatat tggtgatgga tttaacggtg ttaattgccg caatgccatc tgcgccgccg
    29041 cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc
    29101 attggcagcg tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt
    29161 tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg
    29221 atcatatccg cgccagctgc tcccattgct gctcattttc ccccatgatc gaagcgatca
    29281 atactttgtc cgggtaatct tccttcagcc gacgcagggc ggtcagattc tcttccaacg
    29341 gatgttcagc aatctgctcc atatttttga agccgataaa accggtatct tctttcacca
    29401 gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt ttaaacacca
    29461 caccgcccca acctgtgtcg taggctttgg cacacatctc atagcagttg cctaccggcg
    29521 aagaagagag gcagaacggg ttgggaaagt tcacgccgca aaaagtaatc gaaagatctt
    29581 tcgttaacat gagcaagctc cctctaaata gtgatgaatc gcctcggcgg cttctttccc
    29641 ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag cagcaaagac
    29701 ttgcggatcg cggatctgat aattttgtgt tttgatggtg ttacgctgct gctctaactc
    29761 cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat ctgccgccat
    29821 cgtcagttcg cctgataacc gtacatgctt aaacgtgact ttattgcctt ccacggctac
    29881 tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa actctttttc
    29941 gcttgccgga aactcatcta actcttcacg tgctacgcaa gttaccgcct gacagccgag
    30001 aactttcagc gtgctggcta cgtccatcgc gacatcaccg ccgccgataa ttaatgcgct
    30061 ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt tgcaggaagt cgacggcaat
    30121 ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg cttgataacc ccacggtgac
    30181 cagtaccgcg cggttttccg ctttcagttg ctccagggtg agtgttttac cgatttcgtt
    30241 attgcactta atggtcacgc ccattttttc aatacgggcg atctctgcat ccagcactga
    30301 ttgcggtaac cggaattgcg gaataccgtt acgcaaccag ccaccggggt gcgcttcttt
    30361 ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc acactggcct gcaatccggc
    30421 aggacctgcg ccaataatcg cgactttgcc gagcgtttta gtaccgggct gataaatttc
    30481 catcccggtt tgttgttcaa aatcagtaac aaaacgctgt aagcggccga tatcaatggg
    30541 agcatcaaca ccggcacggg tacaaccgct ttggcataat ttctccgtcg ggcacactct
    30601 ggcacaaacg gcaccgaggg cattattttc gcgaattgtc tcggcagcgc ctttaaaatt
    30661 acgaaagtag attgagcgaa taaatttccc cggatcggtc tgtgccgggc aagcctgact
    30721 acagggagcg tcgtgacata ataaacagcg agaggcttct ttaatcgcca gtaacggcgt
    30781 aaaagccgga gtgagttcat ccagataatt ttgttgcggc atgtgatctt ccttaatgct
    30841 atgagtaatt atggcgaacg ctaaataaat attgcgtgct gatattaagg ctatgcgcgt
    30901 ggggaaattt aagctgtgaa aggattcaca aatagctgcg gttcagaatg ttatatccgc
    30961 agcttattga gtgaagtaat aattattctc aataattaac agaaaagaga aaatattaac
    31021 gatagaaaat aagataaaca atcatgccga tagataaaat tccgaagcca atatatgcca
    31081 ccatataagc gaagaagagt ccgggatttt ttgctcgcca gacatacacc ggttccggtg
    31141 catttttaac ccgtttatca atcgcgcctg cgcgccagcc tttccatgct tcgcggaaac
    31201 aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa ctgctgtaca tccataacta
    31261 ctttccttgt ttcttttgta attcaagtaa ctgttcgggt gtaacggcgg gatacccctg
    31321 cgcatcttcc ggcacctggt gcatagccgg aatagggacc agcggcccta aaaaacgcgg
    31381 ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa cgggcggcaa attcgcgaat
    31441 acgtactgac agcatatctt tcggtgacgg tacggcataa cactgagcct gaatccccat
    31501 atgcagcgca ataaataatg ctcgctcaca gtggaaacgt tgggtgataa taatgaaatc
    31561 attagtatcg aaaactttgc gtgtacgcac gatggaatcc agcgtacgaa agcctgcgta
    31621 atcgagaaca atatctgatg ggtcgacacc agcagcgatt aaatctttgc gcatggtcat
    31681 cggctcgtta taactttgca atgcgttatc gccgctcagt aatagataat ttaccttacc
    31741 gctgttatag gcattaatcg ctccttgaat gcggtagcga taatactgat taataacgcc
    31801 ggtacgataa tattttgctg tgccgagcac cacgccgacc tggcggtagg ggagatcctg
    31861 caattcgtcg tagatataag gcgcggtttt ccagctcatc cagcgatcga ggccgagcac
    31921 agtcaacagc agcaagccga tcaggactaa caggctgagg aacacgcgct ttaacatgca
    31981 ggagactcga gctaaggaaa atttccatca ggctacttta cccgctggtt ttgcgcaaga
    32041 aacggcaaac tgtttgccgg gaatttcgac accaggagag ggctggcaag ccggacaagg
    32101 cgttcacgcc gcatccggca ccaggcttaa gcgagaagca cgcggtcgat actgtgacag
    32161 ccgagagctt tcagcgttgc ggaggtggca tcccactgaa tcaacggcgc atcggctttt
    32221 tctgccagaa ccgcgcgctg gatatccggg taatcataac ccttgagatt caacagaatt
    32281 aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg cgttttcagc gtagctgccg
    32341 ctgaaaatac gaccgtcttt acattccagc gcgacaccgc ttggtgactt actgtaaggc
    32401 atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa gcgcatcacc agtcagcgca
    32461 tagccgtgat cctgctcgtc catcagcagc gttttaatct ccagatcttt cggcccaaag
    32521 gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc ccggcaaatg aatacgcaga
    32581 tccagaccgc tgttcagttc attcataaac tgacggcagt gaccacaagg cgtatagtta
    32641 acggtgatgg ctgcaagcgc tttttcacca ctcaaccaag cgtggctgat cgcgctttgt
    32701 tcggcatgaa cggtttgctg cattgttgca ccaataaatt ccatattggc accaaaatac
    32761 caggttccgc tcacaccgcg cgcaattgcg ccaacattaa aattcgacaa tggcgtacgc
    32821 gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg cgtcttcgtc cagtcccgtt
    32881 gcgctcttca gcgatgagac ttgctccccg gtcaacaaag cggggaagta cttgtctgcc
    32941 agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt gggcaaaagc ggtttgaaaa
    33001 cgtggatgca tggcgttgcc tcataatcat gtaatgggat aacagtttac gaacccgtta
    33061 tggctttata tgtgatctga atctcattaa ttatgcaaca cgtgaaactt acatcaaaaa
    33121 tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa taatcgggaa aaggaacggt
    33181 gcgatcagcg aagtaattat cccgcataac accagcgcta gcgaactaaa tgcaccttcc
    33241 tgataatcca gctcggcgca gcgcgccgta ccgagggcgt gcgaggcagt ccccattgcc
    33301 agaccgcgcg cagctttggt acgaatacgc atcgcattaa gcaatgtatg gccaaatacc
    33361 gcgccgagga tgccgacgaa aatcacgcaa actgcgctga ttgccggaat accgccaata
    33421 ctgccgccaa ccgccattgc aataggcgtg gtgactgatt tcggcaggat tgacgcggcg
    33481 atttccggtg aagcgcccat caacaatgcc acggaagtcc ccgtcaccat cgcaaccacg
    33541 ctgccgataa aacagatggt gatgatcgat ttccagcgcg cgcggatctg gtgtagctgc
    33601 tcatataaag gataggccag cgcgacgacc gccggttgca gcagatcgtt tagcacttcg
    33661 ctacctttaa agtaactgtc gtaagagatg ccggtcagca ttaaaaaagg aatgatcacc
    33721 accattgcta ccagcaacgg gttaagcagg ggaaacttat accgtgccgc cagtttgcgg
    33781 gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc agatattcgc catcattctt
    33841 ctgatccttt ctgacctacg actttacgtt caccgtgtac cagttgtgaa ctccagctca
    33901 ccaccagaaa aaccaccagc gtactgacag cacaggaaac cactaccggg ccaaactgcg
    33961 cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat cggcacaaac aatagcgcca
    34021 tatagcgaat cagtacgtag caccccggat tgacccattt cgccggaaga atttgcaagg
    34081 ccagcaggac aaacaggatc agcatcccga tgatgctgcc cggaatggtt accggtagca
    34141 gggaagcaat gaaaatgcct gcatacaggc aggcataaat caggacgaaa gcgcgtaaat
    34201 attgccagat aatgttcagt gtcttactca taatcaacac cccagatgag atggattcat
    34261 catacaatta attcgtaaaa cgtgctaccg atcacattaa gaaatcagcg cttatttttt
    34321 cgctacccgg tattacgcaa tgagcagcga actcaggcat acatcaagca tagagaggga
    34381 ttgcagtttg cagcaatccc tctttgcaga cctttaatcc gctgaccata ggcgctaaca
    34441 tagcgcctca tttttttgcg ggtgatgata tgcgtgtgtt actggcaccg atggagggag
    34501 tgcttgactc tctggtgcgt gaattgctga ccgaagttaa cgactacgat ctgtgcatca
    34561 ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa agtctttcat cgcatttgcc
    34621 ctgagctaca aaacgccagc cggacaccat ctggtacgct ggtgcgcgtg cagttgttag
    34681 gtcagttccc acaatggctg gcagagaacg ccgcccgtgc ggtggagtta ggtttctggg
    34741 gcgtggatct caattgcggc tgcccgtcga aaacggttaa cggtagcggc ggcggggcga
    34801 cgttactcaa agatcctgaa ctcatctacc agggggcaaa agcgatgcgt gaagctgtac
    34861 cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg ggacagcggt gagaagaaat
    34921 ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga gctggtggtg catgggcgga
    34981 cgaaagagca gggttaccgc gcggagcata ttgactggca ggcgattggc gagattcgcc
    35041 agcggctgaa tattccggtg attgccaacg gtgaaatctg ggactggcag agcgcgcaac
    35101 aatgcatggc gatcagcggc tgcgacgcag tgatgattgg tcgcggggcg ctcaatattc
    35161 ccaacctgag ccgggtggta aaatataacg aaccgcgaat gccgtggccg gaggtggttg
    35221 ctttgctgca aaaatatacc cgtctggaaa agcagggcga taccgggtta tatcacgttg
    35281 cgcggattaa acagtggttg agttatttgc gtaaagaata cgatgaagca acggaattat
    35341 ttcagcatgt tcgggtgttg aataactccc ctgatattgc aagggctatt caggcaattg
    35401 atatcgagaa actctaaaaa ccatagtgat gtttttgaca atgtgttagc tctttatcaa
    35461 taggccgcac ggttgtttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc
    35521 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc
    35581 ggtagccgta agggataagc cgagggcacc gcgcagcggc gattttgttc gccagagccc
    35641 gggggggcag ggggcggcgg cgattggtcg ccccctgcgc gctccttgcg ccagtgatta
    35701 tatgaagcaa tgcacatgac aggagcgcaa caggatgaga gactgaaata ttctcgttat
    35761 tttcggatct gatgacatta attaatttta aaatatcatc ttaaatacgc cagtcaccac
    35821 cagtagccca atcaggaaaa taatcaatac agcccagagt ataattttca ttggcgttcc
    35881 tttatttgtg agtgctgaat aagtatagga aaaaacgaag ccctttgccg ttatccataa
    35941 acgattaaga taaatcggaa gatatcattt acttagtcta tatttttctg ggtatgtcgt
    36001 tttattgcta ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac acacggtaat
    36061 ctccgcgcac cgtttcttac ctgattattc ccccatgaat cgtgattcct tttatccagc
    36121 catcgcctgt tttccgctgt tactgatgct ggccgggtgt gcgcctatgc atgaaacccg
    36181 ccaggcgtta agccagcaaa cgcccgctgc acaagttgac accgcattac ccacggcgct
    36241 gaaaaatggt tggccagaca gccaatggtg gctggagtat cacgataatc agctcacttc
    36301 attaattaac aatgcactac agaacgcacc ggatatgcag gtggctgaac agcgcattta
    36361 gctagctgaa gcccaggcga aggcagttgc cactcaggat ggtccgcaga tcgacttttc
    36421 ggcggatatg gagcggcaaa aaatgtcggc agaagggcta atggggccgt ttgctctgaa
    36481 cgatccggcc gcaggtacga ccggtccgtg gtacaccaac ggaacttttg gcttaacggc
    36541 gggctggcat ctcgatatct ggggaaagaa tcgggcggag gttactgccc gcctgggtac
    36601 ggttaaagca cgggcggcgg aacgcgagca aacccgccaa ttgctggctg gcagcgtagc
    36661 ccgcctgtac tgggagtggc aaacccaggc ggcgttaaac acggtcttgc agcaaataga
    36721 aaaagagcag aacaccatta tcgcgaccga tcgccagcta tatcagaacg ggattacttc
    36781 ttcagttgac ggtgtggaaa ccgatattaa tgccagcaaa acccggcagc agctcaacga
    36841 tgtcgcgggg aaaatgaaaa ttattgaggc acggttaaat gcactcacaa atcaccagac
    36901 taagtcattg aagcttaaac cggtcgcgtt gccgaaagtg gcaagccagc ttcctgatga
    36961 actggggtac tccttactgg cccggcgggc agatttgcag gcggcgcact ggtacgttga
    37021 gtcatcgtta agcactattg atgcggcaaa agcggcattt tatcctgaca tcaacctgat
    37081 ggccttcctg caacaggatg cgttgcactt aagcgatctg ttccgtcatt ccgcgcagca
    37141 aatgggcgtt acggcaggcc tgacgctacc tattttcgat agtggtcgtc ttaacgccaa
    37201 tctcgatatc gcaaaagccg aaagcaactt gtctatcgcc agctacaaca aagcggtggt
    37261 tgaagcagtg aatgacgtgg cgcgggcagc cagtcaggtt cagacactgg cggagaaaaa
    37321 ccagcatcag gcgcaaattg agcgcgatgc cttgcgtgtg gtaggtcttg cgcaggcccg
    37381 ctttaacgcg ggcatcattg ctggttcccg cgtcagcgaa gccagaatcc cggcgctgcg
    37441 tgagcgggcc aatggcctgt tattgcaagg gcagtggctg gatgcctcca ttcgactcac
    37501 cggtgcgctg ggcgggggtt acaaacgctg atgatatgcg ccttctatac ttaacgttta
    37561 ttcagcgtta agtggagaac tcgatggcat aggtcgcgat aattaccgct tccgattcgg
    37621 ggatcggcaa agagtgcgcg ttattactgg cgcagcaggg gtttgatatt ggcattacct
    37681 ggcactcaga tgaagaaggg gcaaaagata ccgcgcgtga ggtggttacc cacggcgtac
    37741 gtgcggagat cgtgcaactg gatctcggca atctgccaga aggtgcgcag gcgctggaga
    37801 cactcatcca gcgatttggg cgtattgatg tgctggtgaa taatgcgggt gcaatgacca
    37861 aagcgccgtt tcttgatatg gcttttgatg agtggcgcaa gatttttacc gttgatgttg
    37921 atggtgcatt tttatgctcg caaattgcgg ctcgtcagat ggtgaaataa gggcagggcg
    37981 gtcgcatcat caacattacg tcggtacatg aacatacgcc gctgccggat gccagcgcct
    38041 acacagccgc taaacatgcg ctcggcgggt taaccaaaac gatggcgctg gagttggtca
    38101 ggcataagat tttggtgaac gcagtcgcgc ctggggcgat agccacgcca atgaacagca
    38161 tggatgatag cgacgtgaag cccgacgcgg agccttcgat tcctctacgg cgttttggcg
    38221 caacgcatga gattgccagc ctggtggtgt ggctttgttc ggagggcgca aattacacga
    38281 cagggcagtc gttgatagtg gatggcggct ttatgttggc gaatccgcag ttcaacccag
    38341 aatagcgcag tgaggtgcgt ggatatttca atgggttagg ccagataaga caccagcgtc
    38401 gcatcaggca tggaacaccc ggtgccggat gtggagtgaa tgcttatctg tggcaactgc
    38461 tttacgcctg attatccggc tttttcttcc cgcgccgttt cagccaccag cgcacgccca
    38521 ccaccagaac cacgaccaga atcaaccaga cccagtgctt caaatgctgg tcgagattgt
    38581 gcaaccacgg cgcaatcacc tgaccaccag cgtaaccaat agtggtaaaa atcaacgccc
    38641 aggcaaatgc gccgagaata ttcagcggca gaaagatttt cggcggcagc tggctggcac
    38701 caatcagcgt cgggccaatc acccgaaagc catacataaa gcgcgtacca atgacaaaca
    38761 gatacggatg gcgttggata agtttctgtg cccgctcaat tttatcctga tgtttcgaga
    38821 aacggcgtaa cagcttgccg ccaaaccgcc gcccgcacag atagagcacc tggtcgccaa
    38881 tcatgccacc aagcgccaca gaaagtacca ccagtgggaa ctttaatagc ccctgatgtg
    38941 ccgcaacgcc tcccagcaaa gtcacggttt caccttccgc caggctaccg ataaccagcg
    39001 cggcataacc atattgtgag atgagtgtat tgagatccat aggccagttt gtctcccttt
    39061 ggttgcctgt aagcatacac cctgtgaatc attcacgctg aaaaaggaca tacatcagac
    39121 ggtgcaaatt acatggatga acaaaaaata atcacaagct ggcttatact tgaactgtca
    39181 ggttcatgaa ctcacaacaa gggggcggta tgagccatgt ctggggactg ttttcccatc
    39241 ccgatcgtga aatgcaggtg attaatcgcg aaaacgaaac gatttctcac cattacaccc
    39301 accacgtttt gctgatggcg gcgattccgg tgatttgcgc cttcattggc actacacaga
    39361 ttggctggaa ttttggcgat ggcactatcc tgaagttatc ctggtttacc ggactggcgc
    39421 tggctgtctt gttttacggc gttatgctgg ctggggttgc ggtgatgggg cgggtcatct
    39481 ggtggatggc gcgcaattac ccgcagcgtc cgtctcttgc gcactgcatg gtcttcgcgg
    39541 gctacgttgc aactccgctg tttttaagcg gtctggtggc gctttatcca ctggtctggt
    39601 tgtgtgcgct ggtcggcacg gtggcgctgt tttacaccgg ctacctgctt taccttggta
    39661 ttccgagctt cctgaatatc aacaaagagg aaggccttag cttctccagt tcgacgctgg
    39721 cgattggcgt gctggtactg gaagtgctgc tcaccctaac cgttatacta tggggctacg
    39781 gttatcgttt gttctagaaa gttctttgac gttgcattgc tggcgtaaat gtcagcaatg
    39841 cagcatttac tcacgattct cctttgacga tcgcactttt tgctcgttat gatggcgtcg
    39901 ctagcctcag taaatcctta ctccggcggt gcgcaacccg tgcgcgtgaa ccactatctg
    39961 aatgctcatc atgccgaaat ttcgagtttc tttatttagc ttggccctga tgctggctgt
    40021 gccttttgca ccgcaggccg ttgcgaaaac ggcagccgct accaccgctt cacaaccgga
    40081 aattgcctcc ggtagcgcga tgattgttga tctgaatacc aacaaagtga tctattcgaa
    40141 ccacccggat ctggtgcgtc cgattgcgtc tatcagcaaa ttaatgaccg cgatggttgt
    40201 gctggatgca cgactgccgc tggatgaaaa actaaaagtg gatatcagcc agacgccgga
    40261 gatgaaaggg gtctattcgc gcgtacgact gaatagcgaa atcagccgta aagatatgct
    40321 gttgctggcg ctgatgtctt cagaaaaccg cgcggcggca agccttgcgc accattatcc
    40381 cggtggttac aaagccttta ttaaggcaat gaatgcgaaa gcgaaatcgc tcggaatgaa
    40441 caacacgcgc tttgttgaac ctaccggatt gtcggtgcat aacgtttcaa ctgcccgtga
    40501 cttaaccaaa ctgctcattg ccagcaaaca atatccgttg atcgggcagt taagtactac
    40561 ccgggaagat atggcaacct tctctaaccc gacgtatacg ctgccgttcc gcaatactaa
    40621 tcatctggtg tatcgcgata actggaatat tcagttaacc aaaaccggct ttaccaatgc
    40681 ggcgggccat tgtctggtga tgcgtacggt tatcaataat aaaccggtgg cgctggtagt
    40741 gatggacgcg tttggcaaat atacccattt tgccgatgcc agccgcctgc gtacctggat
    40801 tgaaaccggt aaagtgatgc ctgtgccggc agcagcgttg agctataaaa aacaaaaagc
    40861 cgcccaaatg gcggcggcgg ggcagacggc acagaacgat taagtgaatt cggatggcga
    40921 tactctgcca tccgtaattt ttactccact tcctgccagt ttttccgttt actggtttta
    40981 ccgatccccg gattcatgct attggtcgga tcattctcgc gatagaactt ctgcaacgtc
    41041 tccggtgctt tatacaaatg accgacgtta tgctcggcag ggtactgcgc gccgcgctgc
    41101 tgtagcagtt ccagcatctg ttcttttaac gcatgcacat ccacgccttt tttcactatg
    41161 taatcctgat ggaagacata gcacataaaa tggccgtaat agagcttgtg caccagctgg
    41221 ctgtcgatct ccggcggtaa atgctcatac cactcggtgt cgttacgccg cagagcgata
    41281 tccagtgcca gaatgtcttc gacttcatcg gaatgcaccg cctgataacg aattgctgcg
    41341 cccgcagcgg cgaaacggtg taaaaacgct ttgctacctt cctccggcgt acagacaaag
    41401 aaatcgccct cggcctgttt gaaataatcc accagccacg atttggcttc accgacgcca
    41461 tcgcctgcca tttttaacag cagatgatgc tcgtatttat cgcgccagtt tttcatgcgc
    41521 ggcggtaaat ggctggggaa caggtgaccg aatttttgca ttgcacggtc agtaaaatgc
    41581 ggacggaaga atttcacttt ctccagcatc gcatcggtgc gtcccttgag attaaagaag
    41641 aacggcatct tgtcggtgcc taacttatca atcatcagga aggtgtcttt gccgtatttt
    41701 tccgcaatat cgtagatatc ccggtgcata tattccccgg caaccggcag attttcgaag
    41761 ttagccagaa tatgacggcg gatttcggtc agcacttccg gctggttggt gccgatataa
    41821 aacacctgct gatttttttc cgcttcgaag gtatcaagac gtaccgcaaa gacggccagc
    41881 ttcccggcgc aaccagaaga ttcaaataac cgatccggat cggcgttata acgtgcgggc
    41941 gtgtcggctt caatatcacg aacgcggtgg acatagtcat aatcgtgggc gtgacgacca
    42001 tcgtgacgca catcgtcatc tttgatgcga tcgtcatcca gcttgctaag gatctgctcc
    42061 ggcgtttcgc ccagatcaat ccccagatgg ttcaccagcg tcagtttgcc atcttcatct
    42121 atacgcgcga ataacgacat ttcggtatac gccgggccac gttgcaccag cgagccgccg
    42181 gagttgttac aaataccgcc gatgaccgat gcgcctatac acgatgatcc aatcaccgaa
    42241 tgcggttcgc gtcccagcgg tttgagggct ttttccagcg aatagagcgt ggtgcccgga
    42301 taggccagca cctgttcgcc cttgccgaga acgtgcagct tgtcgagacg cagggtgctg
    42361 atgatcacga tatcgcgatc atagtcgtta ccgtttggcg tcgatccttc ggtcaggcct
    42421 gtattggcag cctgcatcag aataattttg tcggcggtga cgcaggcttt cagcacccgc
    42481 cacaattcca gtagtgagcc agggaaaacg acagccagcg cgtcgccctg accagaacgg
    42541 aagcccttgc gatagcgggc cgtttttgcg ggatcggtga gcaggtgtga atgaccgacc
    42601 agacgggcaa gttcattcaa aaaggcttta ttatcagttg ttgtcatgga agacattttc
    42661 cactccttgt ggtggcgaaa aaatatcaca aactagaata gcgccttgta tgacaattca
    42721 gcgagtgaat attttgaaaa ttcagacaat aaactgagca ttctttttct ctctccatca
    42781 tgcttatggc aaactacggt ttttgcacca ttttcgctgc cacgtcgggc aacaaaggaa
    42841 gaaaaatcca tatgaaatgg ctatgttcag taggaatcgc ggtgagtctg gtcctgcagc
    42901 cagcactggc ggatgattta ttcggtaacc atccattaac gcctgaagcg cgggatgcgt
    42961 tcgtcaccga tctgctcaag aaaatgacgg ttgatgagaa aattggtcag ctgcgcttaa
    43021 tcagcgtagg cccggataat ccgaaagagg cgatccgcga gatgatcaaa gacggtcagg
    43081 ttggggcgat tttcaacacc gtaacccgtc aggatatccg cgccatgcag gatcaggtga
    43141 tggaattaag ccgcctgaaa attcctcttt tctttgctta cgacgtgctg cacggtcagc
    43201 gcacggtgtt cccgattagc ctcggtctgg cctcgtcttt taacctcgat gcggtgaaaa
    43261 cggtcgggcg tgtctctgct tatgaagcgg cagatgatgg cctgaatatg acctgggcac
    43321 cgatggtcga tgtctcgcgc gatccgcgct ggggacgtgc ttccgaaggt tttggcgaag
    43381 atacgtatct cacctcaata atgggtaaaa ccatggtgga agcgatgcag ggtaaaagcc
    43441 cggcagatcg ttactcggtg atgaccagcg tcaaacactt tgccgcatac ggcgcggtag
    43501 aaggcggtaa agagtacaac accgtcgata tgagtccgca gcgcctgttt aatgattata
    43561 tgccgccgta caaagcgggg ctggacgcag gcagcggcgc ggtgatggtg gcgctgaact
    43621 cgctgaacgg cacgccagcc acctccgatt cctggctgct gaaagatgtt ctgcgcgacc
    43681 agtggggctt taaaggcatc accgtttccg atcacggtgc aatcaaagag ctgattaaac
    43741 atggcacggc ggcagacccg gaagatgcgg tgcgcgtggc gctgaaatcc ggaatcaaca
    43801 tgagtatgag cgacgagtac tactcgaagt atctgcctgg gttgattaaa tccggcaaag
    43861 tgaccatggc agagctggac gatgctgccc gccatgtact gaacgttaaa tatgatatgg
    43921 ggttgtttaa cgacccatac agccatttgg ggccgaaaga gtctgacccg gtggatacca
    43981 atgccgaaag ccgcctgcac cgtaaagaag cgcgtgaagt ggcgcgcgaa agcctggtgt
    44041 tgctgaaaaa ccgtctcgaa acgttaccgc tgaaaaaatc ggccaccatt gcggtggttg
    44101 ggccactggc ggacagtaaa cgtgacgtga tgggcagctg gtccgcagcc ggtgttgccg
    44161 atcaatccgt gaccgtactg accgggatta aaaattccgt cggtgaaaac ggtaaagtgc
    44221 tgtatgccaa aggggcgaac gttaccagtg acaaaggcat tatcgatttc ctgaatcagt
    44281 atgaagaagc ggtcaaagtc gatccgcgtt cgccgcaaga gatgattgat gaagcggtgc
    44341 agacggcgaa acaatctgat gtggtggtgg ctgtagtcgg tgaagcacag gggatggcgc
    44401 acgaagcctc cagccggacc gatatcacta ttccgcaagg ccaacgtgac ttgattgcgg
    44461 cgctgaaagc caccggtaaa ccgctggtgc tggtgctgat gaacgggcgt ccgctggcgc
    44521 tggtgaaaga agatcagcag gctgatgcga ttctggaaac ctggtttgcg gggactgaag
    44581 gcggtaatgc aattgccgat gtattgtttg gcgattacaa cccgtccggc aagctgccaa
    44641 tttccttccc gcgttctgtc gggcagatcc cggtgtacta cagccatctg aacaccggtc
    44701 gtccgtataa tgccgacaag ccgaacaaat atacttcgcg ttattttgat gaagctaacg
    44761 gagcgcttta tccgttcggc tatggtctga gctataccac tttcaccgtc tctgatgtga
    44821 aactttctgc gccgaccatg aagcgtgacg gcaaagtgac ggccagcgtg caggtgacga
    44881 acaccggtaa gcgcgagggg gccacggtag tgcagatgta cttgcaggat gtgacggctt
    44941 ccatgagtcg ccctgtgaaa cagctgaaag gctttgagaa aatcaccctg aaaccgggcg
    45001 aaactcagac tgtcagcttc ccgatcgata ttgaggcgct gaagttctgg aatcaacaga
    45061 tgaaatatga cgccgagcct ggcaagttca atgtatttat cggcactgat tccgcacgcg
    45121 ttaagaaagg cgagtttgag ttgctgtaat gctttagtaa gggcgcgacg tttgcgccct
    45181 ttgcctgatg ttcatatctt aacttgctga attttcgccc tttgtaggcc ggataaggcg
    45241 ctcgcgccgc atccggcagg aaacgccgga ccgaatatgc ctcttaagta tttatctgat
    45301 tctgcaactg aatccttccg ctcaggctaa ctctgtcatt atcaactatg cttttctctt
    45361 aattcgctga aaacaacagt aaaagaatga ggaaagcatc atgccactct taaagctctg
    45421 ggcaggttca ctggttatgt tggcagccgt gagcctgccg ctacaggcgg cttcccccgt
    45481 taaagtcggt tcaaaaatcg ataccgaagg cgcgctgctc ggcaacatca tcttgcaggt
    45541 actcgaaagc cacggcgtac cgacggtgaa taaagtgcaa ctcggaacga ctcctgtggg
    45601 gcgcggggcg attacttccg atgaactgga tatctatccg gaatataccg gcaatggcgc
    45661 tttcttcttt aaagatgaaa acgatgcagc gtggaaaaat gccgggcagg gctacgagaa
    45721 agtcaaaaag ctcgatgcgg agcacaacaa gttaatctgg ctgacgcccg cgcctgcaaa
    45781 taacacctgg actatcgccg tgcgtcagga tgtggcagag aaaaacaaac tcacttcgct
    45841 tgccgacctg agtcgttatc tgcaagaggg cggcaccttc aaactggcag cctcggcaga
    45901 gtttatcgaa cgcgccgatg cgttacccac gtttgaaaaa gcctatggct ttaagctcgg
    45961 tcaggatcag ttgctgtcac tggctggtgg cgacacggcg gtgacgatta aagccgctgc
    46021 ccagcaaacc tctggcgtta atgccgcaat ggcttacggc actgacggtc cggtcgcggc
    46081 gctggggctg caaaccttaa gcgatccgca aggcgtgcaa cctatctacg cgcctgcacc
    46141 agtggtgcgt gaatcggtgt tgaaggagta tccgcaaatg gcacagtggc tacagccagt
    46201 cttcgccagc ctcgatgcaa aaacattgca gcaactgaat gccagcattg cagtggaagg
    46261 actggatgcc aaaaaagtgg ctgccgacta cctgaaacaa aaagggtggg cgaagtaatt
    46321 tcccgtgact tatctacgta ttaatcctgt tctggcgctg ctgttgttgc tgacggcaat
    46381 cgcagcggcg ctgccgttta tcagttacgc gcctaatcgt ttagtctcgg gtgaagggct
    46441 tcatctctgg caactgtggc cgcaaacgat ctggatgctg gtgggcgttg gttgcgcctg
    46501 gctgacagcc tgttttgtcc ccgctaaaaa aggcagcatt tttgcactca ttctggcgca
    46561 attcgtcttc gtattgttgg tgtggggagc tggaaaggcg gcgacccaac tggcgcaaaa
    46621 tggcagtgcg ctggcgcgta ccagcctcgg cagaggtttc tggctggctg cggcgctggc
    46681 attgctggcc tgtagcgatg ccatccgccg aatctccacg catccgctgt ggcgctggtt
    46741 gttgcatatg cagattgcca ttattccgct gtggttgctg tactccggca cgcttaacga
    46801 tctctcacta atgaaagaat acgccaaccg tcaggatgtg tttgacgacg cgctggcaca
    46861 acatctgacg ttgctgtttg gtgcggtgct gcctgcgtta gtgattggtg tgccgttggg
    46921 catctggtgc tacttttcca ctgcgcggca gggggcaatt ttttctctgc tcaatgtcat
    46981 tcagaccgtg ccttcggtgg cgctctttgg cctgttgatt gcgccgcttg ccgcgctggt
    47041 tacggccttt ccgtggctgg ggaagctcgg catagcagga accggaatga cacccgcact
    47101 gattgcgctg gtgctctatg ccttgctgcc gctggtgcga ggcgtggtag tcggtttgaa
    47161 ccagatcccg cgcgatgtgc tggagagcgc cagagcgatg gggatgagcg gggcgcagcg
    47221 tttcctgcat gttcagttac cgctggcgtt accggtattt ctgcgcagcc tgcgggtggt
    47281 gatggtgcaa actgtaggta tggcggtgat tgcggcgtta atcggcgcag gcggttttgg
    47341 tgcgctggtt ttccaggggc tgctaagcag cgccattgat ttagtgttgc tgggggtgat
    47401 cccggtaatt gttctggcgg tgcttaccga cgcgctgttc gatttgctta tcgcactgct
    47461 gaaggtgaaa cgtaatgatt gaatttagcc atgtcagcaa actgttcggc gcacaaaaag
    47521 ccgttaacga tctcaatctc aattttcagg aagggagttt ttcggtgctg attggcacat
    47581 ctggctccgg caaatccacc accctgaaaa tgattaaccg cctggtggag catgacagcg
    47641 gagtgatccg ctttgccgga gaagaaattc gctcgctgcc agtactggag ttgcgccgcc
    47701 ggatgggcta tgccattcaa tctattggcc tgttccccca ctggagcgtg gcgcaaaaca
    47761 tcgctaccgt gccgcaatta caaaaatggt cgcgggcgcg gattgacgat cgtatcgacg
    47821 aattaatggc gctactgggg ctggagtcaa atttgcgtga gcgttatccg catcagcttt
    47881 ccggtggtca gcagcaacgt gtgggagtgg cgcgtgcact ggctgccgat ccgcaagtct
    47941 tactgatgga tgaacctttt ggcgcactgg acccggtaac gcgcggcgcg ttgcaacaag
    48001 agatgacgcg cattcaccgt ttgctggggc gcactattgt gctggtcact catgatattg
    48061 atgaggcgct acggctggca gaacatctgg tattgatgga tcacggtgaa gtggtgcagc
    48121 aggggaatcc gctgacgatg ctgactcgtc cggcgaatga ttttgtccgc cagttttttg
    48181 gacgtagtga actgggggtg cacctgcttt cgttacgtag cgtggcggat tacgtgcgtc
    48241 gcgaagaacg ggcagaaggt gaggcactgg cagaagagat gacgctacgc gatgcgctct
    48301 ccctgtttgt cgcgcgggga tgcgaggtgc tgccggtggt gaacacgcag ggccagcctt
    48361 gcggcacgct gcattttcag gatctgctgg tggaggcgta agcgtatgaa gatgttgcgc
    48421 gatccgctgt tctggctcat tgctcttttt gtggcgctga ttttctggct gccttacagc
    48481 cagccgctgt ttgctgcctt gttcccacaa ctgccacgac ccgtttatca gcaagaaagt
    48541 tttgcagctc tggcactggc tcatttctgg ctggtgggaa tttcgagttt gtttgcggtg
    48601 atcattggca caggtgccgg aattgctgtt actcgcccgt ggggcgcgga atttcgccca
    48661 ctggtggaaa ctattgccgc cgttggacag acttttccgc ctgtcgcagt gctggcgatt
    48721 gccgttccgg tgatcggctt tggtctgcaa ccagcgatta tcgccttgat cctttacggt
    48781 gtgctgccca tcctgcaggt gacacttgcc gggctgggag cgattgatcc cagcgtgaca
    48841 gaagttgcga aaggtatggg aatgagtcga ggtcagcgac tgcgtaaggt cgagctaccg
    48901 ctggcggctc cggtgattct ggcgggcgtg cgaacttcgg tgattatcaa cattggtacg
    48961 gcgacgatcg cctcaacggt aggggccagt acgctgggta cgcccatcat catcgggctt
    49021 agcggattta ataccgcgta tgtgatccag ggggcgttac tggtggcact ggcggcgatc
    49081 atcgcagacc gcctgtttga aaggctggtg caggcgctta gccagcacgc aaaataaagg
    49141 tataacctgc gagcatgacg ccaccaattc cgcctaacgc cataaacagg aacagggcga
    49201 tgaccccaat tttagctatg cgcataatgc actccttatg ttaacgaaag gattgtacag
    49261 taaagcgcat ttgttaacga atcattaaat accgagtggg aaaatatcat ggccttgttc
    49321 ttgccaactg gtgagttgct gctgttgggc ggaggttcga ttttcaccgc accacaccag
    49381 caatgtacgg ccttcgaata gttcagggcg tagttgattg agcgagtggg cgagcgagga
    49441 catcaatgcg ccatccttgt tgactggcaa tccagccctc cagccacaga cgggtggtat
    49501 cctgaatatt ccagccaacc accagcgcat ctttaccctg ttttttacgt gccgaagcca
    49561 gacaaatggc gatgtagttg atcagtacgc cgtcgaggat cgccagcagc gcctggagag
    49621 tcggttgttg gcactgaagc cgtcggcgca gaggaataaa cagatgtgtg gtgagtgtct
    49681 gggcggggta atcctgaccg cgctctttga tccacgttcg caggctatgt agattgccg
//
LOCUS       DADLEE010000019        48321 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000019 DADLEE010000000
VERSION     DADLEE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.019"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            187..480
                     /locus_tag="JQH51_001178"
     CDS             187..480
                     /locus_tag="JQH51_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6075587.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQH51_001179"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQH51_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY6075588.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="JQH51_001180"
     CDS             complement(1922..2374)
                     /locus_tag="JQH51_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY6075589.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /locus_tag="JQH51_001181"
     CDS             complement(2427..3761)
                     /locus_tag="JQH51_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6075590.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="JQH51_001182"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="JQH51_001182"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY6075591.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQH51_001183"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQH51_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY6075592.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQH51_001184"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQH51_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY6075593.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQH51_001185"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQH51_001185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY6075594.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQH51_001186"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQH51_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY6075595.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQH51_001187"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQH51_001187"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY6075596.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQH51_001188"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQH51_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY6075597.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQH51_001189"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQH51_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY6075598.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="JQH51_001190"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="JQH51_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY6075599.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="JQH51_001191"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="JQH51_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY6075600.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="JQH51_001192"
     CDS             complement(15085..15582)
                     /locus_tag="JQH51_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY6075601.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="JQH51_001193"
     CDS             complement(15579..15941)
                     /locus_tag="JQH51_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY6075602.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="JQH51_001194"
     CDS             complement(15931..16278)
                     /locus_tag="JQH51_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY6075603.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="JQH51_001195"
     CDS             16387..16800
                     /locus_tag="JQH51_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075604.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18313)
                     /locus_tag="JQH51_001196"
                     /pseudo
     CDS             complement(16847..18313)
                     /locus_tag="JQH51_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="JQH51_001197"
     CDS             complement(18324..20039)
                     /locus_tag="JQH51_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY6075605.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="JQH51_001198"
     CDS             20206..21099
                     /locus_tag="JQH51_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6075606.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="JQH51_001199"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="JQH51_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(21910..23526)
                     /locus_tag="JQH51_001200"
     CDS             complement(21910..23526)
                     /locus_tag="JQH51_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY6075607.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="JQH51_001201"
     CDS             23821..24495
                     /locus_tag="JQH51_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6075608.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="JQH51_001202"
     CDS             complement(24534..26195)
                     /locus_tag="JQH51_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY6075609.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="JQH51_001203"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="JQH51_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY6075610.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="JQH51_001204"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="JQH51_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY6075611.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            27649..27981
                     /locus_tag="JQH51_001205"
     CDS             27649..27981
                     /locus_tag="JQH51_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY6075612.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29251)
                     /locus_tag="JQH51_001206"
     CDS             complement(27983..29251)
                     /locus_tag="JQH51_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY6075613.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTNTNTNTWTPDSQWL
                     VFDVRPSGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPEN
                     PDETWHYDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFT
                     YNDHVMHQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSD
                     EINRAYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTET
                     TLPAPPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLI
                     SPQGGEPRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANP
                     PSADAVVFSPDGQWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="JQH51_001207"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="JQH51_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY6075614.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="JQH51_001208"
     CDS             complement(30377..31714)
                     /locus_tag="JQH51_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6075615.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="JQH51_001209"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="JQH51_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /locus_tag="JQH51_001210"
     CDS             complement(32934..33551)
                     /locus_tag="JQH51_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY6075616.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /locus_tag="JQH51_001211"
     CDS             complement(33535..34413)
                     /locus_tag="JQH51_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY6075617.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="JQH51_001212"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="JQH51_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY6075618.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="JQH51_001213"
     CDS             35377..36033
                     /locus_tag="JQH51_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075619.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="JQH51_001214"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="JQH51_001214"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY6075620.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37412)
                     /locus_tag="JQH51_001215"
     CDS             complement(37005..37412)
                     /locus_tag="JQH51_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY6075621.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="JQH51_001216"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="JQH51_001216"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY6075622.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="JQH51_001217"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="JQH51_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY6075623.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="JQH51_001218"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="JQH51_001218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY6075624.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="JQH51_001219"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="JQH51_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY6075625.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="JQH51_001220"
     CDS             43964..44044
                     /locus_tag="JQH51_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075626.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="JQH51_001221"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="JQH51_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY6075627.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="JQH51_001222"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="JQH51_001222"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY6075628.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="JQH51_001223"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="JQH51_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY6075629.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="JQH51_001224"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="JQH51_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY6075630.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="JQH51_001225"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="JQH51_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY6075631.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DADLEE010000020        47956 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000020 DADLEE010000000
VERSION     DADLEE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.020"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="JQH51_001226"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="JQH51_001226"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY6075632.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="JQH51_001227"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="JQH51_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY6075633.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="JQH51_001228"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="JQH51_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY6075634.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="JQH51_001229"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="JQH51_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY6075635.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="JQH51_001230"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="JQH51_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY6075636.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="JQH51_001231"
     CDS             5377..5835
                     /locus_tag="JQH51_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY6075637.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="JQH51_001232"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="JQH51_001232"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY6075638.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="JQH51_001233"
     tRNA            6887..6971
                     /locus_tag="JQH51_001233"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="JQH51_001234"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="JQH51_001234"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="JQH51_001235"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="JQH51_001235"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY6075639.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="JQH51_001236"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="JQH51_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY6075640.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="JQH51_001237"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="JQH51_001237"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY6075641.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="JQH51_001238"
     CDS             complement(10252..13995)
                     /locus_tag="JQH51_001238"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY6075642.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="JQH51_001239"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="JQH51_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY6075643.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="JQH51_001240"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="JQH51_001240"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY6075644.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="JQH51_001241"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="JQH51_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY6075645.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="JQH51_001242"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="JQH51_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY6075646.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="JQH51_001243"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="JQH51_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY6075647.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /locus_tag="JQH51_001244"
     CDS             complement(20534..21229)
                     /locus_tag="JQH51_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY6075648.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="JQH51_001245"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="JQH51_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY6075649.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="JQH51_001246"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="JQH51_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY6075650.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="JQH51_001247"
     CDS             23392..23499
                     /locus_tag="JQH51_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY6075651.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="JQH51_001248"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="JQH51_001248"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY6075652.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="JQH51_001249"
     CDS             complement(24538..25347)
                     /locus_tag="JQH51_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY6075653.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="JQH51_001250"
     CDS             complement(25351..25743)
                     /locus_tag="JQH51_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY6075654.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="JQH51_001251"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="JQH51_001251"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY6075655.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="JQH51_001252"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="JQH51_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY6075656.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="JQH51_001253"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="JQH51_001253"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY6075657.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="JQH51_001254"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="JQH51_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY6075658.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="JQH51_001255"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="JQH51_001255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY6075659.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /locus_tag="JQH51_001256"
     CDS             30792..31739
                     /locus_tag="JQH51_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY6075660.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="JQH51_001257"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="JQH51_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY6075661.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="JQH51_001258"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="JQH51_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY6075662.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="JQH51_001259"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="JQH51_001259"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY6075663.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="JQH51_001260"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="JQH51_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY6075664.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="JQH51_001261"
     CDS             36790..39657
                     /locus_tag="JQH51_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6075665.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41685)
                     /gene="dhaR"
                     /locus_tag="JQH51_001262"
     CDS             complement(39757..41685)
                     /gene="dhaR"
                     /locus_tag="JQH51_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY6075666.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="JQH51_001263"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="JQH51_001263"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY6075667.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="JQH51_001264"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="JQH51_001264"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY6075668.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="JQH51_001265"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="JQH51_001265"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY6075669.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /locus_tag="JQH51_001266"
     CDS             45366..47063
                     /locus_tag="JQH51_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY6075670.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="JQH51_001267"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="JQH51_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12041 a        12343 c        11743 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DADLEE010000021        47311 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000021 DADLEE010000000
VERSION     DADLEE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47311
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.021"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..481
                     /gene="sseA"
                     /locus_tag="JQH51_001268"
     CDS             <1..481
                     /gene="sseA"
                     /locus_tag="JQH51_001268"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY6075671.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1300..2076)
                     /gene="sseB"
                     /locus_tag="JQH51_001269"
     CDS             complement(1300..2076)
                     /gene="sseB"
                     /locus_tag="JQH51_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY6075672.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2218..3501)
                     /gene="pepB"
                     /locus_tag="JQH51_001270"
     CDS             complement(2218..3501)
                     /gene="pepB"
                     /locus_tag="JQH51_001270"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY6075673.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3560..3760)
                     /gene="iscX"
                     /locus_tag="JQH51_001271"
     CDS             complement(3560..3760)
                     /gene="iscX"
                     /locus_tag="JQH51_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY6075674.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3772..4107)
                     /gene="fdx"
                     /locus_tag="JQH51_001272"
     CDS             complement(3772..4107)
                     /gene="fdx"
                     /locus_tag="JQH51_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY6075675.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4109..5959)
                     /gene="hscA"
                     /locus_tag="JQH51_001273"
     CDS             complement(4109..5959)
                     /gene="hscA"
                     /locus_tag="JQH51_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY6075676.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5976..6491)
                     /gene="hscB"
                     /locus_tag="JQH51_001274"
     CDS             complement(5976..6491)
                     /gene="hscB"
                     /locus_tag="JQH51_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY6075677.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6587..6910)
                     /gene="iscA"
                     /locus_tag="JQH51_001275"
     CDS             complement(6587..6910)
                     /gene="iscA"
                     /locus_tag="JQH51_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY6075678.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6927..7313)
                     /gene="iscU"
                     /locus_tag="JQH51_001276"
     CDS             complement(6927..7313)
                     /gene="iscU"
                     /locus_tag="JQH51_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY6075679.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7341..8555)
                     /gene="iscS"
                     /locus_tag="JQH51_001277"
     CDS             complement(7341..8555)
                     /gene="iscS"
                     /locus_tag="JQH51_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY6075680.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8667..9155)
                     /gene="iscR"
                     /locus_tag="JQH51_001278"
     CDS             complement(8667..9155)
                     /gene="iscR"
                     /locus_tag="JQH51_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY6075681.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9425..10165)
                     /gene="trmJ"
                     /locus_tag="JQH51_001279"
     CDS             complement(9425..10165)
                     /gene="trmJ"
                     /locus_tag="JQH51_001279"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY6075682.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10284..11087
                     /gene="suhB"
                     /locus_tag="JQH51_001280"
     CDS             10284..11087
                     /gene="suhB"
                     /locus_tag="JQH51_001280"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY6075683.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11232..12086
                     /locus_tag="JQH51_001281"
     CDS             11232..12086
                     /locus_tag="JQH51_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6075684.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12277..13557
                     /gene="csiE"
                     /locus_tag="JQH51_001282"
     CDS             12277..13557
                     /gene="csiE"
                     /locus_tag="JQH51_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY6075685.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13549..14688)
                     /locus_tag="JQH51_001283"
     CDS             complement(13549..14688)
                     /locus_tag="JQH51_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY6075686.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14848..15738)
                     /gene="hcaR"
                     /locus_tag="JQH51_001284"
     CDS             complement(14848..15738)
                     /gene="hcaR"
                     /locus_tag="JQH51_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY6075687.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15874..17235
                     /locus_tag="JQH51_001285"
     CDS             15874..17235
                     /locus_tag="JQH51_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HAY6075688.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17232..17750
                     /locus_tag="JQH51_001286"
     CDS             17232..17750
                     /locus_tag="JQH51_001286"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY6075689.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17750..18070
                     /locus_tag="JQH51_001287"
     CDS             17750..18070
                     /locus_tag="JQH51_001287"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY6075690.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18067..18879
                     /locus_tag="JQH51_001288"
     CDS             18067..18879
                     /locus_tag="JQH51_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY6075691.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18889..20106
                     /locus_tag="JQH51_001289"
     CDS             18889..20106
                     /locus_tag="JQH51_001289"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY6075692.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20203..20625
                     /locus_tag="JQH51_001290"
     CDS             20203..20625
                     /locus_tag="JQH51_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY6075693.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20673..21545)
                     /locus_tag="JQH51_001291"
     CDS             complement(20673..21545)
                     /locus_tag="JQH51_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY6075694.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21557..22651)
                     /locus_tag="JQH51_001292"
     CDS             complement(21557..22651)
                     /locus_tag="JQH51_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY6075695.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22684..23682)
                     /locus_tag="JQH51_001293"
     CDS             complement(22684..23682)
                     /locus_tag="JQH51_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6075696.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISDEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23707..25218)
                     /locus_tag="JQH51_001294"
     CDS             complement(23707..25218)
                     /locus_tag="JQH51_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6075697.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25241..26223)
                     /locus_tag="JQH51_001295"
                     /pseudo
     CDS             complement(25241..26223)
                     /locus_tag="JQH51_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            complement(26320..29421)
                     /locus_tag="JQH51_001296"
     CDS             complement(26320..29421)
                     /locus_tag="JQH51_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY6075698.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            29719..30912
                     /locus_tag="JQH51_001297"
     CDS             29719..30912
                     /locus_tag="JQH51_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY6075699.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31110..32363)
                     /locus_tag="JQH51_001298"
     CDS             complement(31110..32363)
                     /locus_tag="JQH51_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY6075700.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32690..33880
                     /gene="hmpA"
                     /locus_tag="JQH51_001299"
     CDS             32690..33880
                     /gene="hmpA"
                     /locus_tag="JQH51_001299"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY6075701.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33925..34263)
                     /gene="glnB"
                     /locus_tag="JQH51_001300"
     CDS             complement(33925..34263)
                     /gene="glnB"
                     /locus_tag="JQH51_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY6075702.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34324..35658)
                     /gene="glrR"
                     /locus_tag="JQH51_001301"
     CDS             complement(34324..35658)
                     /gene="glrR"
                     /locus_tag="JQH51_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY6075703.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35648..36361)
                     /gene="qseG"
                     /locus_tag="JQH51_001302"
     CDS             complement(35648..36361)
                     /gene="qseG"
                     /locus_tag="JQH51_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY6075704.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36526..37953)
                     /gene="qseE"
                     /locus_tag="JQH51_001303"
     CDS             complement(36526..37953)
                     /gene="qseE"
                     /locus_tag="JQH51_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY6075705.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38511..42398)
                     /gene="purL"
                     /locus_tag="JQH51_001304"
                     /gene_synonym="purI"
     CDS             complement(38511..42398)
                     /gene="purL"
                     /locus_tag="JQH51_001304"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY6075706.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42657..44213
                     /gene="mltF"
                     /locus_tag="JQH51_001305"
     CDS             42657..44213
                     /gene="mltF"
                     /locus_tag="JQH51_001305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY6075707.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44210..44746)
                     /gene="tadA"
                     /locus_tag="JQH51_001306"
     CDS             complement(44210..44746)
                     /gene="tadA"
                     /locus_tag="JQH51_001306"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY6075708.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44771..45406)
                     /gene="yfhb"
                     /locus_tag="JQH51_001307"
     CDS             complement(44771..45406)
                     /gene="yfhb"
                     /locus_tag="JQH51_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY6075709.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45615..46463
                     /locus_tag="JQH51_001308"
     CDS             45615..46463
                     /locus_tag="JQH51_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY6075710.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46802..47293)
                     /locus_tag="JQH51_001309"
                     /pseudo
     CDS             complement(46802..47293)
                     /locus_tag="JQH51_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436854.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11279 a        12962 c        11899 g        11171 t
ORIGIN      
        1 acttgcaggc tggcagcgcg atgacctgct attagaagaa ggtgcagtag agctgccgga
       61 aggagagttt aacgccgcgt ttaatcctga agcagtggtg aaagtaaccg atgtgttatt
      121 ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac gttttaacgc
      181 agaagttgat gaacctcgcc caggtttacg tcgcggacat attcccggtg cattgaacgt
      241 tccgtggacg gaactggtgc gcgaaggcga actaaaaacg accgatgaac tggatgcgat
      301 attttttggt cgcggcgtca gctatgacaa accgattatc gtcagctgcg gctctggtgt
      361 aacggcagcc gtggttttgt tagcactcgc gacgctggat gtgccaaacg tgaaactgta
      421 cgacggcgca tggagtgaat ggggcgcgcg ggcagattta ccggttgagc cagtgaaata
      481 agtatattac aggcaataaa aaaccgccga atttggcgtt tttttattgc tggtctggtt
      541 cgcggccttt ccagcaggtt gacttgtgtt acatgagcaa cgcaggtgct tcacagcaaa
      601 acaatactca ccagtaactc tctttttgtc aagcaaaaga gagtaattat tgtttattta
      661 gcgtattatc gacaccggcc ctttccgccg tgttcggtaa taaaataacc tggcttatta
      721 gtccgaattc agacaaatat aaacaaatcc tgctcaaaat taaaaattct aaccggtaaa
      781 agatattact taaacatgta aatttacttt cctttaaaaa acaaaaaacc gccaaaatca
      841 ggcggttatt tgttgctggt ccggttcgcg gcctttccag caggttgtat taccgtagta
      901 atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa
      961 gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa
     1021 gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc
     1081 ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca
     1141 caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag
     1201 taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca
     1261 gcaagtaagc ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt
     1321 aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat gttcggtaat
     1381 aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc
     1441 aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc
     1501 ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat
     1561 aaaagcacgc ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg
     1621 tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg
     1681 gctgctcagc ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc
     1741 tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa
     1801 tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc
     1861 taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc
     1921 aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt
     1981 ggattccagt agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc
     2041 cagcaggtct tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt
     2101 cggggcgcaa atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt
     2161 gaatttgcag aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta
     2221 cgccgttaac agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg
     2281 ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata
     2341 gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc
     2401 gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc
     2461 cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagta aacgaccggc
     2521 aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc
     2581 cccggtgagg gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag
     2641 accatcggca agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc
     2701 gttgcgatag gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc
     2761 acagcagagg aacagcttca cgcgcttgtt cagtccgcgc ataatggcaa atgccagcgc
     2821 cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg
     2881 tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac
     2941 cggtgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc
     3001 tgaaccgcgc ccgacagtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt
     3061 ggtgatccga taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg
     3121 tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc
     3181 gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc
     3241 cactttacgc gtgcctttcg ggcctttata accttgccag aatgcccagc agcgatccgc
     3301 atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgggc
     3361 cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt
     3421 attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt
     3481 aatcttcatc gcttctgtca ttttagttat ccttattcgt ttaacagcaa aacgggctac
     3541 ccccaggcag cccgttcatt cactcggctt cgtccagcca gactaacaaa atcgcttcga
     3601 ggattttctc gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact
     3661 gatgcatatc ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg
     3721 cttcgccaat ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc
     3781 acgcgcatgg ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtgacgcg
     3841 cgcctggcag cttaaacggc tttccggctc cagtccccag gctttgtcca gcatgtcgtc
     3901 ttcctgctct gagctttccg gcagtgagtc aaaaccttca cgaacgatgc agtggcaggt
     3961 ggtgcaagca caggattttt cacaggcgtg ttcaatctca ataccgttac gcagcgctgc
     4021 gtcgagaatg gtttcaccgc tattagcttc cagaacagcg ccatcagggc agaggtcctg
     4081 atgaggcaaa ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg
     4141 cacgacgaac cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt
     4201 ttttaatcgc ttgttcgatg gcgtcaacat catcgccctg cgccacttca ctcaggtgag
     4261 cggcagcatt gtcaatgacc tgacgctctg cggcgcttaa cagcgcggca tcagcagcca
     4321 gcgcgccgtg cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcatac
     4381 gggcttttac gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc
     4441 tatcggtcag accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct
     4501 ccatcgccgt cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg
     4561 caccgccagc cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt
     4621 cctgcaccag ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt
     4681 taaaggtggt gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt
     4741 tctccaccag gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca
     4801 gcatttcgct gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag
     4861 cgacgacttt atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc
     4921 gttcacgcac cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt
     4981 cagcttctac acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt cgttttacca
     5041 gtggcgcgat cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt
     5101 taacggtcac ggagtccgca tcgctcagcg cgattttggc tgcaatggtg gcatccagca
     5161 gttcacgctg aacgcggtta tcgctacgat caggaatgtc cgcctgctcg cgaatgtaat
     5221 ccgccagcag atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca
     5281 cttcaaacac gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt
     5341 cataaacagc gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcag
     5401 tcggttcgtt aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc
     5461 cctgacgctg ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc
     5521 ctgccagggc ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca
     5581 ccgggttcag tagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga
     5641 attgataagg cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc
     5701 gtttaacaga actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac
     5761 ccaccgaatg cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat
     5821 ggtcggctaa cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc
     5881 ccaggtcaat accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac
     5941 taatttgtaa taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg
     6001 agttgttcgg cactgctccg cagtttatcg agaaaacgca gcttacgcac cgtatccgcc
     6061 gccgcgtccc acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac
     6121 atctttttca cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc
     6181 tcgtccagct cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta
     6241 tgctgctcgc tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac
     6301 ggatgacgca gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt
     6361 tgttccgcca ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga
     6421 aaacgcaggc tcagtgcctg ggtatcgagt tgataacggg caggcaagcc aaagagggtg
     6481 aagtaatcca taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa
     6541 caaaccccac gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc
     6601 tttcgccgca accacactca tctttaacgt tcgggttggt gaatttaaac ccttcattca
     6661 ggccttcttt tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga
     6721 ccacgacttt cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa
     6781 attccagtac ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa
     6841 accctttgcc gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg
     6901 taatcgacat aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca
     6961 atggcggctt tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt
     7021 tcaagttctt cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag agacttccct
     7081 ttcacccatt cggtgaccag ggagctggaa gcgatagcgg aaccgcagcc gtaagtttta
     7141 aaacgcgcgt cttcaatgat accttcatcg ttgactttaa tctgcaactt cattacgtcg
     7201 ccacaggccg gtgcacccac catgccgctg ccgacgttct cgtcgttgtt atcaaaggaa
     7261 cccacgttac gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat
     7321 tctcctgatt ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc
     7381 tgcttgtaca tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact
     7441 aactcgatgg tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata
     7501 gagctatgtg ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg
     7561 cttgctgacg tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc
     7621 gactcacctt caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg
     7681 tcaccgttca ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg
     7741 ccgcgcagac gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcttcgccc
     7801 attccgacga tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg
     7861 ccgtgcattt gcgcttcgat gcgtacacgc ggtttacgac gcacatacag cgcaccgata
     7921 cctttcgggc catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc
     7981 aggtcgattg gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga
     8041 gcacggcaca tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg
     8101 tgcatgatgg aaacaaggat ggtgtcgtca cgcatcgccg cttcgagttc tttcaggtcg
     8161 ataataccgt tacgctgcgg tgccaggtag gtgacttcga aaccttcgcg ctccagctga
     8221 cggcaagtat ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt
     8281 ttctgataaa agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag
     8341 gtaaagacga tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata
     8401 tctaccgctt cttcagcctg ccagccgaaa cggtgagaac gagaggccgg gttaccaaag
     8461 gttccgtcca tcgtcatgaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg
     8521 gttgcggagt agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact
     8581 caatgcaagg aatcaggcta ccggctggat gtacgaccgt gtttacggag tatttagcac
     8641 tccggcctga ttctgaattc tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt
     8701 gtgcgggtgc gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg
     8761 ttattaacca gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa
     8821 tcacgccaca gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga
     8881 caacgggtgg catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg
     8941 ctggcatctt tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca
     9001 tttttacgca gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg
     9061 gaaatatcag ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg
     9121 gtcacggcat agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc
     9181 ctgcccgggg ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat
     9241 tcccgagtaa attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt
     9301 gccatttttt tatcgtggcg tttgtacctg ttgtcggatg cggtgtgaac accttatccg
     9361 acctacgatt ctgtacattg taggcctgat aagacgcgtt aagcgtcgca tcaggcaacc
     9421 actgttattc ggccttgtta cctttattct gctgctcaat agaagccaga atcccgcgca
     9481 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
     9541 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat
     9601 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
     9661 gctcgccatt ttcctgagtt gccagccagg ccatacgcac ttcataggca ataacctgaa
     9721 ccgccattgc caggttcagc gaactgtatt ccgggtttgc cgcaatcgcg acatgataat
     9781 ggcatttctg caactcttca ttggtcaagc cgacgcgctc acgaccaaac accagcgcca
     9841 ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
     9901 gccacggcag cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
     9961 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgcagcca
    10021 gggcaatcgc ctgggagtcg ggtttcacca gtggattaac cagccacaga ttggttaatc
    10081 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
    10141 gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt
    10201 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
    10261 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
    10321 gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
    10381 agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
    10441 acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg
    10501 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggtact accaacttta
    10561 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
    10621 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcaaggcg
    10681 cacagctgaa cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc
    10741 tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
    10801 gcaagctgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
    10861 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt
    10921 tcgcggcagg cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg
    10981 gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgtgtt gtgaaagcca
    11041 tactggcgaa catgcgtgac gagttaagcg acgctctgaa gcattaatga ctcaggcggg
    11101 tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt
    11161 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc
    11221 aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct
    11281 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    11341 ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta
    11401 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaacg
    11461 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    11521 tggtcagttg gttacctgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    11581 gtaaatcaaa aggcactccg tcccaggccg gattgctgga cgatacgcaa agcgccatca
    11641 atgtggtgcg ccatcgcagt gatgtaaacc cacaacagct ggtgctgttc gggcagagta
    11701 ttggcggggc gaatattctt gctgttattg gtcagggtga tcgtgaaggc atacgtgcgg
    11761 tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca
    11821 gtggctactt acttgatgag agttacagcg gcgaaaatta catcgccagc gtcagcccga
    11881 tcccgctttt actcattcac ggtaaagccg atcacgttat cccatggcag cacagcgaaa
    11941 agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca
    12001 ttgatgcttt ttccgatcgt cacggcaatg tttatcgcga acagatggtg gactttatcc
    12061 ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa
    12121 ccaccttaag acatgctaat ccactggtca gaacagttta aaatgagaaa aattctgtga
    12181 cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag caaactttag
    12241 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    12301 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    12361 ttgaccgcca ccatggcaac cttcagcgag cttaatggtg tggatgatga tattgccagt
    12421 cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact
    12481 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    12541 cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca attcaccccc
    12601 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat
    12661 ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga atcgcgcgat
    12721 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc agcaccacgc tggaattacg
    12781 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    12841 caggaaattg gacgccactg gcagcgtcgg gcgctccagc atgtaccgcc tgatgagcca
    12901 ctgtttatgg cattactttt ttccatgttg cgggttcccg atccactgcg ggatgcgcat
    12961 cagcgggaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg
    13021 ggaaatgttc gtttttatga tgaacagggg ttatgtgatc agctttatat ccacctcgcc
    13081 caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc
    13141 gccagactgt atccacgcct ggtacgcacc acccgcgcgg cgctggccgg atttgaaagt
    13201 gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc
    13261 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat
    13321 agcgagcgag aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac
    13381 attaagcata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    13441 attattgcgc cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac
    13501 ctgacgctga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    13561 aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatta cccagaacac
    13621 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    13681 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctaa cctggcgagc
    13741 agcaatataa cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg
    13801 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    13861 gcaaatcgcc gagatcaaca gcatatcgcg agcactacaa cggcggaaaa gtttattact
    13921 cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    13981 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    14041 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    14101 cgcagaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg
    14161 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcacga ggatcacccg
    14221 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    14281 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    14341 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    14401 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg gcagcagtgc
    14461 cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa
    14521 actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    14581 taaaccaatc cctttaagcc agacgctcca gaaaggtaaa aaaatgccgt aactaaaaaa
    14641 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    14701 tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt
    14761 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgcgatccgc
    14821 gacgcatgtt gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac
    14881 aatggcgatg aaatcgcgta atgccagctt catctcccct ttcttccacg ccatcagtaa
    14941 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt
    15001 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    15061 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc
    15121 tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc
    15181 atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    15241 caccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    15301 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    15361 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt
    15421 tggcaataaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    15481 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    15541 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    15601 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    15661 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    15721 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    15781 attaactatt tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag
    15841 gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt
    15901 taccaactga ttgacaccca aaacggtcgg gtcactccgc gtatttatac cgacccggac
    15961 atttaccaac tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa
    16021 agccagatcc caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcagttgtc
    16081 gtggtgcgcc agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc
    16141 atgcgtgtga gttatgcaga ttgtggcaac agtcgcgcct ttacctgccc gtatcacggc
    16201 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    16261 caagggttgt gtaaatccca ctgggggcta aacgaagttc cttgtgtgga gagttataaa
    16321 ggactgattt ttggcaactg ggataccagc gcaccgggcc tgcatgatta cctgggtgac
    16381 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    16441 ggcgtacaaa agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt
    16501 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    16561 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    16621 aaagatgcgc tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa
    16681 ccggatgcta atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc
    16741 gaagcagaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatata
    16801 ttccccacgc tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc
    16861 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    16921 aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag
    16981 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    17041 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    17101 ggcattacta actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc
    17161 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    17221 gaggtgatga aatgagtgcg caagtttcac tggagttaca tcaccgcatt agccagtttc
    17281 tctttcacga agccagtctg ctggatgact ggaaatttcg tgactggttg gcacagctcg
    17341 acgaagagat tcgttacacc atgcgcacca cggttaacgc gcaaacacgc gaccgtcgca
    17401 aaggcgtcca gccgccgacg acctggattt tcaatgacac caaagaccag ctggagcggc
    17461 gaatcgcccg tctggaaacg ggcatggcct gggcagaaga gccgccgtca cgcactcgtc
    17521 acttaatcag caactgccag gtaagcgaaa ccgacatccc aaacgtattt gccgtgaggg
    17581 tgaattatct actttatcgg gcacaaaaag agcgcgatga aacattctat gttggaacgc
    17641 gtttcgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    17701 tcctggatca agcggtgatc acttcccata acctgagtgt actgttctga tgaatcgaat
    17761 ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatag atacctcgcc
    17821 cgtcatcgcc ttgttcaacg tgggcggcga gttttatgcc attaacgatc gttgcagcca
    17881 tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    17941 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    18001 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    18061 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    18121 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aagttgccac gctggaactg
    18181 tcggcggcaa aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa
    18241 ggtaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    18301 ggcaagctgg attgttttat cggcaatgca ggcatctggg atcacaatgc ctcactggtt
    18361 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc
    18421 ggttacctgc tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg
    18481 attttcacac tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct gtacaccacc
    18541 agtaaacatg ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg
    18601 cgggtgaatg gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    18661 gggcaaagtg aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    18721 ccgctgcaat ttttcccgca accggcggat tttacggggc cgtatgtgat gttggcatcg
    18781 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt
    18841 cgcggcattc gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa
    18901 acgatcatta ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    18961 caagggttca ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga
    19021 cctccactct cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc
    19081 gctaactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg
    19141 ggtcgcgaca cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt
    19201 tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    19261 tgctttaccc tacaccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    19321 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    19381 cagcgcagcg cagcgcagcg cagatgtaag gtgacagtga ttgaactggc ggcaaccgtc
    19441 atgggccgta atgcaccacc gcccgtgcaa cgctatcttt tacagcgcca tcagcaggct
    19501 ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg tcgatggtga aaaagtagaa
    19561 ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg tgatttacgg tattggtatc
    19621 agcgccaacg agcaactggc tcgcgaggcc aaccttgata ctgccaatgg cattgtcatt
    19681 gatgaggctt gccgcacctg cgatcccgcg atctttgccg gtggcgatgt ggcaatcact
    19741 cgtcttgata atggtgcact acaccgctgc gaaagctggg aaaacgccaa taaccaggcg
    19801 caaattgccg ctgccgcaat gttagggcta ccgctaccgc tactgccgcc gccgtggttc
    19861 tggagcgatc agtacagtga taacttacag tttattggcg atatgcgtgg cgatgactgg
    19921 ctttgtcgtg gcaacccgga aactcagaag gcgatttggt ttaatctgca aaacggcgtg
    19981 cttatcggtg cggtcacgct gaatcagggc cgtgagattc gcccaattcg caaatggatc
    20041 cagagcggca aaacgtttga tgcgaaactg ctgatagatg agaacatcgc gcttaaatca
    20101 ctgtaatcag gataattagc gaatatctca atgcctgggg cgtggcgagg tgcaagagtg
    20161 tgtattacgt ttaaatcaca ttatcttgca aagggattgg ttatgaacac actacgttat
    20221 tttgattttg gagctgcccg ccccgttttg ttattaattg cccgtatcgc cgtggtctta
    20281 attttcatta tttttggttt tcccaaaatg atgggctttg acggtacggt ccaatatatg
    20341 gcctcgttgg gcgcgccaat gccgatgctg gcagcgatta ttgcggtagt tatggaagtg
    20401 cccgccgcga tattaatcgt gcttggcttt ttcacccgtc cgctggcggt gctgtttatt
    20461 ttctacacgc tgggtacggc ggtgattggt caccattact gggatatgac cggcgatgcg
    20521 gttgggccaa atatgattaa tttctggaag aatgtcagta tcgctggcgc gttcttgcta
    20581 ttggcaatta ccggaccggg ggcaatttct ctcgatcggc gttaggaaag atgccggatg
    20641 cggcgtgaac gccttatccg gcattttata aattacagcc actctacccg caacgacatc
    20701 tctgaggttg ttgattcccc tggtgctagc gcaatgagat cacccccttc cgggcgatga
    20761 tgctcatccg gcgcatggct catcggttcc agacagaaga aatcaaacgc atatccttta
    20821 tcaaacgcag ggtcggaaac aaagatgaaa taacacggtg caggtggcgt cgtttccatg
    20881 atgatggcat aaccttcctg cggctgctcg atgcgggcct gaccattcca tccggcaaaa
    20941 ccattgttca cccactgacg cggcaacggc gcgagctggt taaaatccag ttcctgcggt
    21001 agctgctcgc aaaactcacc cgccagccac tgctcccgtt ccagccagta accgctcgcc
    21061 tgcgcctgaa tccgcgtttg tggtgacaac ggaaaataag gatgccagcc ggtaccaaat
    21121 ggcagcgtct ctgccccttg attggtgaca gagagcgtca ccgtcagcgt atccgccgtt
    21181 aaatgaaacg cctgacttac ccgatagtga tagacaccgc tgcgatgttc atacaccaga
    21241 cacaaactat catcgctgtg cgagacacat tgccactcgc ccagccagcc atcgccgtgt
    21301 agatagtgcg catcccactc aacattcggt tgcagttgat actcacgccc ctgccagaca
    21361 aaacggttgc cgctcacccg gtttgcaaat ggcaccagcg gaaaacatga ggcatcggtt
    21421 gccacaccgc tttttttacc aggacgtaag agcggcgtcg tatcgcgcca gaagccttcg
    21481 attacgccgc cctggtcaga aacgtccagc tttagcgacc cgtgggataa ggtatagatg
    21541 gtcatccgct aactccttaa tccgggaagt taatcacaac tttcccgcat ttgccgctcg
    21601 ccatcagcac ataggcatca cctgcctgtt ctagcgagaa gcgatgggta atggcgttac
    21661 gcggccacag cttccagtca gtcaaatcgt gggcgcattt ttccatatgg aacagactgg
    21721 tcacccagga gccgataatc cgccgttgat ggtgcatcag atcagcgctg acctcgaatt
    21781 ccacttttcc ggtttcacca atgtaaacca cccgccccca gtcagcagtg gattgcagtg
    21841 ccagcaagcg acctgcggca ttaccggaac aatcgagcgc aacatccgcg ccaccgtggg
    21901 taagttcagc aattatctgc ggcagacctt cggtggttgc taaatagccg tgatccatcc
    21961 cccctaactg ttttgccatc gccagacgtt ccggcagcat atcaacgccg atgatccgtt
    22021 ttgcaccgcg acctttcgcc agcatcatcg ccatcatgcc gaccggcccc agaccgacca
    22081 ccagcacgtt atcgctgccg gaaacttcgc cgcgcaaaat cccttcatag gccgtgccaa
    22141 cgccgcaact gataaacgca ccatcttcgt agctcagcgc atccggcagg aggatcagat
    22201 ctttttcttc cgccagcaga tattcggcat gaccgccgtc acgctgccag ccgtaagccg
    22261 cttttccttt gccagtacaa gagataggaa agccgcggcg gcagttcggg caaaaaccac
    22321 agccagaaat gtgatacacc agcacgcggt cgccctcttt aaaatggcgg cagccttgcc
    22381 ccatcgccac aatctgcccg cacggttcat gaccgttgat aaatccctgg tataacggtt
    22441 tatcgggtgc cgccgccgtg gcacggtgtt gatgatagat atagtggaca tcgcttccgc
    22501 aaatcccgga ggatttcatt ttgatcagta cctggttaat ccccggcgtc ggcaccgcaa
    22561 cttcccgcag atcgacggtt gaatttccta gtaaataagc agccagcatc gttttcataa
    22621 atccctcatt aacaatacga ttaattttca tccctgcagc acccgcgcca ggggcgttaa
    22681 ggtttagcgt ttcgctttac tgcttcgctg ggtcagcaag atattcgcca gcaccgccac
    22741 cacgatgatg acgccgcgta ccacctgctg gaaaaaggag ttaataccga gcagcaccag
    22801 accgttaccg attagcgtaa tcaccagcac accaagcaat gtaccgaaca gagaaccgcg
    22861 accgccggaa agtgccgtac cgccgaccac gaccgcggcg atgacgtcaa actccagacc
    22921 gtttgcggca cctgcgttac cggaaccgag gcgtgccgcc aacaaaatgc cggtcaccgc
    22981 cgctaataat cccgaaaggg taaagataag aatgcgcact cgacgaacgt taataccgca
    23041 caactgcgcc gccgtggcat taccgccaac ggcaaaaacc gagcgcccga aggcggtttt
    23101 tcggctaatg aacacaaaca gcgcaaacaa caccatcatg atcagcgcgg atactggcac
    23161 accgagaaat tgtccgccca gccagtccag cacctcgttt tcgtcaatcg gcactggcag
    23221 tgcgttggtc ataaacagcc ccattccgcg cagagcgctc cacagcccca gtgtggcaac
    23281 gaaacttggt acgttaaaca cgccgcgcag caccccggcg agcgttccca tcagcgcgcc
    23341 taacagcaac accagcagac aagcaatcgc caacggaact tcaaattgca gcaaaaatgc
    23401 caggcacacc gagacaaaag ccaccatcgg cccaacgctg acatcaattt catcggagat
    23461 aataatcagc gtcatcgccc aggcggcaat cccaatggtg gcggcatcgc gcagcacgtt
    23521 catctggtta ttcaatgaga taaagccagg cgcgttcagg gagaagacca gataaagaat
    23581 ggcaatcacc accagcaaac cgatctcatt aatatggcga ctgacaaatt gtttgagcga
    23641 gacgctcttg ccctgcggca atggtaatga cgaagcatac atcttcaggt tcctcggtaa
    23701 aattcatcag tgcacagaca gaatggcgga catcagctca tccacattga ccggcgagtg
    23761 aaactcctgc gagaacgtgc cgtgctgtaa taacaggatg cggtcacaca ccagcggcaa
    23821 ttcctccact tcactggaga taaacaccac gctttttcct tcggcagcca gctcacggac
    23881 aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgttggct cgtcgagcaa
    23941 caaaatctgg ctggcggcat agacccaacg accgatcacc actttttgct gattgccacc
    24001 agaaagcgtg ccgatgggtg tttcgctact ggcagccttg accgtcatcc gctgcatcac
    24061 ctcttcggtc aggcggcgga tggtggacca ttgcagcaca ccgttggtgc tgattttttg
    24121 ccgattggtc agcactgtat tttcgtcaac gcctagccag ggaatgatcc ccgcttcttt
    24181 gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat cggggcgcat
    24241 gattttctcg ccgttgataa caatttcgcc ctgttcatac gtctccagcc caacaatcgc
    24301 cttcagcaat tcactgcgcc ctgcccccag caggccagca atgccaagca cttcgccacg
    24361 acgtagcgta aagctgatat cctccagctt gggcttatgg cgtaacgcac ggacttccag
    24421 tacggcctga tccacaattt cctgaggggc aaccggcgca atatcaacgt gatcacgccc
    24481 tagcattagc gacacaatat gatgcgtgga ggtgttttcg agcatcacat cgcccgccac
    24541 ctgaccatcg cgcataacgg tggcatagga ggcaatgcgg cggatttctt ccatccggtg
    24601 gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcaccgcac tgatcaccag
    24661 ttcaacttct gcactggcaa gcgagctggt gggttcatca agaatgacca cgcgcggctc
    24721 gcccttcatc acccgtgcaa tttccaccag ttgcttttgc gccgggctta gcgttgaaac
    24781 aagttgttca ggactaacgt caacgcccag cgcctgtaag caacgttggg catcctgcgc
    24841 catttgcagg taatcaatca tgccgttgcg gcgaggccat tgaccaaggc agaggttttc
    24901 tgccaccgtc agcccttcca ccagacttaa ttcctgataa accgcacgaa cccccagttc
    24961 agcggcacgg cgagtcaacg tagcttcgtc accttccagt cgcgtctcgc caatccagat
    25021 atcaccgcta tccgggcgtt cgctgccggt aagcattcga atgagagtcg atttgcccgc
    25081 gccgttttta cctaacagcg cacgaacttc gcctttattg agcgtgaagt taacgttatc
    25141 cagcgcaacg acgccggggt aacgcttatt tcctgccacc acttttgcta ccgggactgc
    25201 ctctgttgcc gtgaacatag ggacctctgc gaatcagcga ttagggcaga ccatcaacgt
    25261 gcgttgccag ccactgttta ccatcttccg ttttggtata gagatcgatt ggcacctgaa
    25321 tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg aaaacagcat
    25381 tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg ttttccagct
    25441 cctgagcaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg gcctgatttt
    25501 gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgactccccc ataatggcgt
    25561 tgagatccgg cgtggagata atcagttttt caccaacgga aatcgcttta tctaaaacag
    25621 tcccttcctg gttagcgaca atttgcgcgc cgggaacgcg ggattttaat acttcttcaa
    25681 atcctttacg tcgctgcaca caaacttcaa aggcttcgca attgatgacg gcaattttcg
    25741 gctggtcaat tttattggca ataaaataat cggcggcagc gttacccagt ttttaccaaa
    25801 ttccagtgga tcgccgacca gatacgccga gacatattta tcgacaccct tttgattaat
    25861 acaggtgttg tagcaaatca ccggaatacc cgcttcactg gcgcgacgaa cggtacggct
    25921 actgccattt tcagacactg ccgataaaat aatggcatcg acattacgcg ccacgagggt
    25981 atcaacaaag gtactttctt tcgaaatatc cccctgggcg ttagtttcaa ttaactgtac
    26041 ctgtactgaa gaatcttttg ccgcatcctg aacgccctga cgcactccag cgtaatatcc
    26101 ctgggtatca aggtatattg caccaatggt catttcttta tccgcagccc tggcaaatag
    26161 tgcgctacct aatagcgtag ccattaataa tagattacgg gttgttctca tttttttagg
    26221 catagtgcct cctgtagggt ttttattaac aacggcttat tctaattatt ttgtgatgag
    26281 cggcagcgcg tgccgctcaa atattacagt taatagggtt tagtgaacgt agttaaaaat
    26341 aaaaggcatc acagtggtga ataatgccgc tttcggccag gccggattaa tcgccgtcaa
    26401 ttcagcgcga gcagactcat attgcgcaac ctccccgagt cccgcggacg ccagcatcgc
    26461 caccatcagg catttttctt tatgctgctg ttgtaaatcg ccatacagcg ataacaggtc
    26521 aggttgtgaa acagcaaaga aatccgcctc aatactggtt ttcgccatct cttgcgccca
    26581 ctgtttcatt tcactaaaca gttgctgtgc ggtttgctgt tcacccagca gtcgcagcgc
    26641 cattccttgc cagaagagat aatcaaccgg ctgatcgttg taataactgt ggatgttaat
    26701 ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcatc gcttcagttt catcgccctg
    26761 cgcgttggcg catatcgcct gccagaacca gatgtcgtta tcagtttgcc ccggtaaacg
    26821 gccttcgctt aaattctccg gataatacag cgcggcatga agcagttcgc tggcctgctg
    26881 cggctctctg gcatcaagat gctgccaggc gcgtaataac tggttgagga taaactgact
    26941 ggtgaccttc ccttccccgc cttcccacgg gtggaattta cgcgtggcga gaatgtccgc
    27001 cgctttgtct gcctgacccg tgagatgcca caaattgagc agttctgcgg tcatgtcgtc
    27061 gcgtttcagc gcaatttcca gattattttc cagacgcgcc agtcgtttct ccggtgtggc
    27121 tccacttaac ttatccagca aatcccgttc gaaaagcaga cgtgcatcct gcggcgcaag
    27181 ctgataagca ttatcaagat aacgcgcggc cagctcataa tcgtgtttct tattccacgc
    27241 atggatcgct aacccgcgcc agccgtcggc aaactccggc gacatctcta cgcaacgttg
    27301 ccataaggca atggctttgt tgtagctacg tttgttgtag tagaagcagg ccagtaaatg
    27361 gcgagcaaac caacactctt caatactctc cagcgccgcc acttcttcca gcgtattcgg
    27421 gaaacggaca aactgcggga agacatcaat ggcttttgcg accagttcgc cacgttcggc
    27481 tttcggcagc aagctggctt gcaggtaaag cggcagcgtg cgctggcagt ccagtgcgtt
    27541 cagcatctct gccgccaggg tgggcattcc ccagttaatc agttgcccgg cggtcatcag
    27601 agcgttaacg tcgcgtccct gacacagacc gcgccactgc gcgagggctg attcgctacg
    27661 accatcgaac cagttcagcc accacagagt ggcgttcagc ggataatcac gcagcagttt
    27721 ctcgcgctgc aaacgcgcgt tgtcctgacg accacttaac accagcagca gattatgcaa
    27781 gcaaagcact tcctgattgg ttgggcaggc acgaagactt tgttggcaaa aatccagacc
    27841 cgcgtcgaag ttatcattac gcgccgccag tcgtgccaga ccataatagc cgcctgcttt
    27901 actattgccg ctccagaccg cacgccagaa atcctcttcg gcttgttgat attgtccctg
    27961 acgttcgtaa gcactggcgc gaatcaaact tgcttgtcca cactgcggtt ttttgttcag
    28021 cgcatgtgca cgtttcagcg cctgactggc atacgccacc gcttgcggga aatctgcgcg
    28081 gttatattcc agcatcgcca gcgccaggtt acagcgataa tccagcgggt ccagcgccac
    28141 gccgcgcagg tagtaatcga acggtgaacg gctggcgtga tgatattgct ccagatgctg
    28201 accgataaac caggcttcat ctgtactggt aatgtcttgt gctgccagtg gcgctttggc
    28261 gacgtccggc aacggcaact cctgcggctg atgttcctga taactcagta caatattgcc
    28321 gtcggcatcg gagagttcaa tggtcagtct gtcaggattg ataccgtgca acacgccctg
    28381 gatggcggtc gctggtgtca gggccacggc atcatcgagc aacgcgttgc atttgccgat
    28441 ttcgcggatc gccagacggt atccgttcaa cggagagatg gcatacagcc cccactcaat
    28501 cccccgctca ctacgctgga gttttatcac cgcatcgcgg gaggcatttt gcaccatgcc
    28561 cagagaatga taaggcagga aatactgctc gaaacgcttc tcttcgtaag catcaagcca
    28621 ggtaaaatca ggctggttat cggcaaaaat accggtcatc agttcgatat acgggccgtt
    28681 attgtcggtc agactcttat cccacgcctg gccaaattca ctgtgtcccc aactccactg
    28741 ttttttacct ggcgcaatat ggtggttggc aacgtgcagc aaaccgccat cttcatcgtg
    28801 acaccacgcg ccgacaaaat cgtactgcga tttttcggcc atatatgagg ttggaacagg
    28861 cacattttta tagcgagaaa tgtccactcc ggcggagtag tccactttgt agtaagtgcc
    28921 ggtggcgatg gggaaagcgg agacggcccg tttgccgtga tcaaacaccg cagtcacatc
    28981 cggtgggaaa acgctctgat gcccttcacc ccctttcact gccgggttga cccaccacaa
    29041 gaaatgacgc ggcgtggcgt tgccgttata gacgcggctg gcgatttcca gtgccgcccg
    29101 gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca tgcatcggct ccgtttcgcc
    29161 gacccacacc gtctgtgcac cgtcttcatg ggcttcgagg gtgaaatcaa cgggcataaa
    29221 ggtggtcggg cgatggtgtt gcggccagtt aaactcaatc ccgccggaga tccacggtcc
    29281 cagcatcccc accagcgcag gtttaatgac ttcattgtga tagacaaaat cgcgctgttt
    29341 cactttatcc catgcgcgat gcacccgacc gcccagttcc ggcaggatca tcactttgat
    29401 gtagtcgttt tccagccaca ccgcctgcta ggatttcagg gttttctgct cgctcagcgt
    29461 atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc tgataaacgc gattttccag
    29521 gaacatggga tgtatatcct gcggcccggt ttcataggtc gggatctcaa cgcgctcttg
    29581 ccacactttt actggagtca tggtgccctc aatattaaca agacatactg aattaaaaga
    29641 tttgtggcag tgtattgaac aatctggcaa tgttttcgcg gaataatcac gcaattaact
    29701 aaacaaggtt tagtgaagat gagagcctgc attaacaatc aacagattcg ccaccataac
    29761 aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg ccaataaatc aacgctggcc
    29821 cggctggcgc aaatttcgat tccggcagtc agtaatattt tgcaggaact ggaaagcgaa
    29881 aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg ggcatagtag cggtacatgg
    29941 ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg tgacgcccac cagtattgag
    30001 tgtcaggttg ctaatgcttg tttaagtccg aaaggcgaat ttgagtattt acagattgat
    30061 gtaccgacgc cgcaggcgct gctgtccgaa atcgaaaaat gctggcatcg ccaccgtaaa
    30121 ttgtggccgg accgcaccat caatctggcg ctggcaatcc acgggcaggt tgatccggtg
    30181 accggcgtat cgcaaaccat gccgcaagcg ccgtggacaa cgccagttga ggtgaagtat
    30241 ctgctggaag agaagctggg cattcgggtg ctggtcgata atgactgcgt gatgctggcg
    30301 ctggcggaga aatggcaaaa taattcgcag gaacgggatt tctgcgtgat caacgttgat
    30361 tacggcattg gctcgtcgtt cgtgattaac gagcaaattt atcgcggcag tttgtatggt
    30421 agcggacaga ttggtcacac catcgttaat ccggatggag tcgtctgcga ctgtggacgt
    30481 tacggcagcc tggaaaccgt cgcctcgtta agtgcattaa aaaaacaggc gcgggtatgg
    30541 ctaaaatcac aaccggttaa tactcagctt gagcctgaaa aactgacaac cgcgcagtta
    30601 atcgctgcct ggcagagtgg agaaccgtgg atcaccagct gggttgatcg ctctgccaat
    30661 gccattggtt tgagtctgta taacttcctc aacatcctca atattaatca gatttggttg
    30721 tacggtcgca gttgtgcctt tggtgagaac tggcttaata ctattattcg ccagacagga
    30781 tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag cgacgcaaat tggcttcggg
    30841 caattaagcc gcgcacaaca ggtgctggga attggctatt tgtatgttga ggcgcagtta
    30901 cgacagattt gatggcgcga taacgtagaa aggcttcccg aaggaagcct tgatgatcat
    30961 aaacgaaaaa ttgcctgatg cgctacgctt atcaggccta cacggagatt gcaatatatt
    31021 gaatttgcaa gattttgtag gcctgagaag gtgttcatgc cgcatccaac atgaacaacg
    31081 agcacattgt cagcaaatca ccgtttcgct tatgcgtaaa ccgggtaacg tgcgcagatg
    31141 tcgagaactt tacctttgat gcgctcgata acggcttcat cattgatgct gtccagcacg
    31201 tcacacatcc agccagccag ttctttcgct tcggcttctt taaagccgcg acgggtaatc
    31261 gccggagtac ctacacgaat accggaggtc acaaacgggc tcttcggatc gttcggtacg
    31321 ctgtttttgt tgacggtgat gttagcacgg cccagagcgg cgtctgcttc tttaccggtc
    31381 aggtttttat caaccagatc aaccaggaac aggtggttat cagtgccgcc ggaaaccact
    31441 ttgtagccgc gctcgaggaa cacttctacc atcgctttag cgtttttcgc gacctgctgc
    31501 tggtaagttt tgaactcagg ctccatcgct tctttcagag caaccgcttt accggcgatt
    31561 acgtgcatca acggaccgcc ctgaccacca gggaaaacgg cagagttcag ttttttgtac
    31621 agctcttcgc taccaccttt cgccaagatc aggccgccgc gcggacccgc cagggttttg
    31681 tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt tcgggtagac gccagcagca
    31741 accaggcccg caacgtgcgc catatcaacg aacagataag caccgatgct gtcagcgatt
    31801 tcacgcattt tcgcccagtc aaccacgccg gaatatgcag agaaaccacc gataatcatt
    31861 ttcggcttgt gttctttggc ttgtttttcc agatcggcgt aatcgatatg accggtagca
    31921 tcgataccgt aaggaacgat gttgtacagt ttaccggaga agttaaccgg agaaccgtga
    31981 gtcaggtgac cgccatgcgc caggttcata cccagaacgg tatcacctgg ttccagcagc
    32041 gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt agcgtagtca
    32101 gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc aacatactcg
    32161 caaccgccgt agtagcgttt gcccggataa ccttcagcat atttgttggt cagctgagaa
    32221 ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc
    32281 tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc ataatcggca
    32341 atgttcattt cacgctttaa catccgcatc tcctgactca gctaacaata aaatttttgg
    32401 cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat tgtgcgataa
    32461 caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa ggctttggag
    32521 aataaagagc ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca aatttttcac
    32581 ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg ctatttttta
    32641 taagatgcat ttgagataca tcaattaaga tgcaaaaaag gaagaccata tgcttgacgc
    32701 tcaaaccatc gctacagtaa aagccaccat ccctttactg gtggaaacgg ggccaaagtt
    32761 aaccgcccat ttctacgacc gtatgtttac tcataaccca gaactcaaag aaatttttaa
    32821 catgagtaac cagcgtaatg gcgatcaacg tgaagccctg tttaacgcta ttgccgccta
    32881 cgccagtaat attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa tcgcgcagaa
    32941 gcacaccagc ttccagatca aaccggaaca gtacaacatc gtcggtgaac acctgttggc
    33001 aacgctggac gaaatgttca gcccggggca ggaagtgctg gacgcgtggg gtaaagccta
    33061 tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa atctataacg aaaacgccag
    33121 caaagccggt ggttgggaag gtactcgcga tttccgcatt gtggctaaaa caccgcgcag
    33181 cgcgcttatc accagcttcg aactggagcc ggtcgacggt ggcgcagtgg cagaataccg
    33241 tccggggcaa tatctcggcg tctggctgaa gccggaaggt ttcccgcatc aggaaattcg
    33301 tcagtactct ttgactcgca aaccggatgg caaaggctat cgtatcgcgg tgaaacgcga
    33361 agagggtggg caggtatcca actggttgca caatcacgcc aatgttggcg atgtcgtgaa
    33421 actggtcgct ccggcaggtg atttctttat ggctgtcgca gatgacacac cagtgacgtt
    33481 aatctctacc ggtgttggtc aaacgccaat gctggcaatg ctcgacacgc tggcaaaagc
    33541 aggccacaca gcacaagtga actggttcca tgcggcagaa aatggcgaag ttcacgcctt
    33601 tgctgatgaa gttaaggaac tggggcagtc actgccgcgc tttaccgcgc acacctggta
    33661 tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt gatagcgaag gtctgatgga
    33721 tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg cagttctatc tctgcggccc
    33781 ggttggcttc atgcagttta ccgcgaaaca gttagtggat ctgggcgtga agcaggaaaa
    33841 cattcattac gaatgctttg gcccgcataa ggtgctgtaa tttgatgttg ccggatggaa
    33901 acatccggca acccttgacg cggtttaaat tgccgcgtcg tcctcttcac cggtacggat
    33961 gcgaatgacc cgagccacgt caaagacgaa gattttaccg tcaccgattt tgccggtttg
    34021 cgccgtgcga ataatggtat cgacacaggt atcgacaatg tcgtccggta cgacaatctc
    34081 aattttcact ttcggcagaa aatccaccat atactccgcg ccgcggtaca gctcggtatg
    34141 gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc atgccggtaa taccgacttc
    34201 ggccagtgct tcgcggacat cgtccagctt gaagggtttt ataatcgcat caatcttttt
    34261 catgctattc cttgaaaagg ttgcctgtct tttgatctgc taaacgtaac acataacgcc
    34321 aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg gaaagcagtt tataaaattc
    34381 tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg tgatttgcag
    34441 cagcttacgc aagtagttga gttcaaaatg attacgtgcc tcaacaaagg ttggcagcgc
    34501 cgtattttca ccctccagcg cctgctccac cagcgcatca ctaatcaccg gagatgaggt
    34561 cagcgccacg cactgttcaa tcacgttgac caactggcgc acattacccg gccagctcgc
    34621 ggtcatcagg cgtttcatcg catcggtaga gaacgcgcgg acaaacggtt tatgtcgctc
    34681 tgccgcctgg cgcaacaggt gatttgccag tagcggaatg tcttctgtgc gctccgccag
    34741 tgccggaatt ttcaggctga caacgttgag gcggtaatag agatcttcgc ggaattcccc
    34801 gcgcgccatc gcttttgaca gatcacggtg agtggcagaa ataatccgca cattgatatc
    34861 aatatcgcgg ttactgccca gcgggcgcac tttacgctcc tgcaacacgc gcagcagttt
    34921 gacctgtaac ggcgcaggca tatcgccaat ttcatcgaga aatagcgttc cgccttccgc
    34981 cgcctggaat aaaccttcgc gattgctgac agcgccagta aacgcgccac gcgcatgacc
    35041 aaacagctcc gactccagca attgctcggg caatgcgcca cagttaatag caataaatgg
    35101 tttgctgttg cgcgggctgg cgttgtggat agcctgggcg aaaatctctt tcccggtgcc
    35161 gctctgaccg ttaatcaaaa cgctgacgtc tgattgcgcc accagccgcg cctgttccag
    35221 caaacgcagc atcagcgggc tgcgggtgac aattgcctcg cgccagcgtt catcggtggc
    35281 tggcgcggat tgctccagcg catcgtcaat tgcctgatat agcgcatctt tatcgacagg
    35341 cttggtgagg aaactaaaaa cgccctgctg tgttgcagca acggcatcgg ggatagaacc
    35401 atgcgcagta agaataatta ctggcattcc cggctgcact ttctggattt cagcaaatag
    35461 ctgcataccg tccatttcat ccatccgcag gtcgctgata actaaatcta ctttttcgcg
    35521 attcagtacc cgtaatcctt cagcgccact ttccgccgtg accacactgt agccttcgct
    35581 ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg tcatcgacca ataataaatg
    35641 cgcaggttta tggctcatca ggagtgacct catgggtgga tggcgcgggc ttttcactct
    35701 cgtgcggcgt atccggcgag aaatttccag ccggtttgcg ggtcgagagc tggcgttcaa
    35761 tatcggtcag gttttccagc ttgcgggtgg tgagttccag ttgctgctgt agaacgtgat
    35821 gttgctggcg caatgtatcc agctcgctgt cgctggactg ctggagttta ctgtaacgtt
    35881 ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc gcgccagagt tgatacagtg
    35941 ggcgaacctg tgccgggatc tcggtactta acgcttcaat acgcgcgacc agctggcggc
    36001 gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag gtattttgcc
    36061 agctgccgtc gtcatattgg cgagcttgct gacgcgactg cgcaggcatt aaacgatcag
    36121 cacaatccat cgcccgcagc cagtaaagcg gattggtttc ggttgattta ccttgcagcg
    36181 cccagatgtc gctacattca gtagaaagat agtcagccag ttgataaacc ggaatttttt
    36241 cttctgctgg cgtatcaatg gctggcttat tgtgattctg cacgcaaccc agcaatgcca
    36301 gacatggcag ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa atgtgtcgca
    36361 tattcaccag acttaaagcc tatcccagtg ggcgtaattg ttgcagacag tctggacatg
    36421 gactgcgcgg agaaaccggg gcgtacatat cgtacgtgaa gatttcgagc actacccggg
    36481 gccgaactga caaataaaat agcctgatag gataggctgt tagcattatt tcgtgttttt
    36541 cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga ccagatacag
    36601 ttccccttgc atacggcgaa tacaatccct ggcaatgctt aatcccagac cgctgccctt
    36661 caccgcccct tttcgctggt ggcttccctg aaaaaagggt tcgaagatca tggcgcgttc
    36721 ctcttgcgga atgggcgtgc ctgtattgat gacatcaata taaacccgcg caccatgtaa
    36781 actgctgcga aggcaaatgt taccggattc agccccgtag tgcaccgcat tggagtaaag
    36841 attatccagt acgctcatca gcagcattgg ctccgccagg caagctgttg ctttgagatc
    36901 gacgtcggta tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa ccactgtctc
    36961 caccagcggt gctaattcaa cattctccag ttccaccgca ctgtccgcct gtttacggtt
    37021 gtaatcaagc agttgttcga tcagtttttg caaattgcag ctgctgctat caagaatgct
    37081 caccacctct ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca gtaattcagt
    37141 gccctcgcgc atactcgcca gtggcgtttt taattcatga gataaatgtc ttaaaaattg
    37201 atggcgttgg gattccagcc atgacaggcg ctcacttaac caaagaatac gttgcccaac
    37261 cgagcgtaac tcgctcggtc cacagaacga aacgctattg cccagagaac gcccttcccc
    37321 cagacggttg atcatgcgct cgatattttt caccggcccg ataatcatcc gcgtgaaaag
    37381 tagcaccatc accagactca ccagaaatag caccagcgat tgccaaccaa aatattgccc
    37441 acgttcggcg atttcacgct gaagttgctg cccacgagag aacaccactg tgcgcgtggc
    37501 ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga cgcgcggcgg cggcagcatc
    37561 gggaccactg ttgttacact gaagttgggc cagattgttc aaatcctgac gcaatgcctg
    37621 gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg agcatttcgc tgtaacgctt
    37681 gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc agcacgcaat actgacggta
    37741 gctacgctcc atctccagcg ccgcgttggt catcgcttca ctacgccggg catcaataag
    37801 cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca ttcaggcttt gccaggcttg
    37861 ccatgccagc accaacaggg gtagtagaat tagcaaaaat gccagcatta ccagttgtcg
    37921 taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta ctctcgtcag acgcgaatag
    37981 cctgatgcta accgagggga agttcagata caacaaagcc gggaattacc cggctttgtt
    38041 atggaataag gcggtgccta actcgacgtt tcgcccgatg gttgatatag ctacgctgat
    38101 atcagaagtt ggacggcagg caccttgttg tgcgtcattc gaattttatg tagcacgtcc
    38161 cgaaggggct gacataagtc ggtgaatgag ccactgctta ctattatgca gtaactgtgc
    38221 caataaagaa aatagtttgg taacgtactg attatacgtt gctttgaggg atttatggct
    38281 tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa cttaagataa
    38341 acttattaga ttgcaataaa atcaatcatt taagtgtcgc cagaaagcga cacggtaaac
    38401 cacccattgt cgtgatttac agacacaaaa aagctcccgg agttgggagc ttatgatagt
    38461 ggttggtgct taatgccgca tccgggctgc aaaaccaatg aactgatgac ttaccccaac
    38521 tgcttacgcg cattgcggaa aatgcgcatc catgggccat cctcgcccca gttttccgga
    38581 tgccaggagt tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact
    38641 cgaccacttt cagtcgtaac cgccgtaata ccgttcggtg aaccgttcgg gttagccggg
    38701 taggtttcag tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt
    38761 tccagtgccg ccagatgcgc cgcatcacgc acttccacgc gcccttcacc atgagagaca
    38821 gcaatcggca tttgcgagcc caccatcccc tgcaacagca gagacgggct ttgggttact
    38881 tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtgt tacgcacaaa acgtggccac
    38941 aactcactcc ccgggatcag ttcacgcagg ttagacatca tctggcaacc gttacatacc
    39001 cccagggcca gcgtttgcgg acgatggaag aaggttgcaa actcatcgcg tacacggtca
    39061 ttgaacagga tagacttcgc ccaaccttca ccggcaccca gcacatcacc gtaggagaaa
    39121 cctccgcacg cgaccagggc gtgaaaatcc tccagtcccg tacgtccagt cagcaggtcg
    39181 ctcatatgca cgtcgatagc atcaaagccc ccacgttgga aagctgccgc catttcaaca
    39241 tgcgagttca cgccctgctc acgcagtacg gcaactttcg gacgtgcgcc agtggcaata
    39301 tacggtgctg ccacatcttc gttgatatcg aacgacagtt ttacattcag gcccggatcg
    39361 gcgtcgttag atttcgcctg atgctcctga tcggcacact ccgggttgtc acgcaggcgc
    39421 tgcatctgcc aggtagtttc tgcccaccag acacgcaacg tagtgcggct ttcgctgaat
    39481 acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa
    39541 tggacgcaat ccgcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg
    39601 gcacgaacct gaatcaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtca
    39661 cccagagagg cgatatccgc gtcaatgcca caatgacctg caaaggccat ttccgccagc
    39721 gttaccagca ggcctccatc agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc
    39781 agcgcctgaa tcgcgtcata gaagcctttc agttggacaa catcgcgtac atctgccggt
    39841 ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct
    39901 ttgcccaaat caatcagcag cagtgcgtta tcttcggtac aaagctgcgg cgtgatggtg
    39961 tgacgtacat cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt
    40021 tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc
    40081 gggatcgtca ggcccagcgc cggacaaagt tcttcaccca cggctttaac ggcttcatac
    40141 aggcccgcat cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg
    40201 cgtttgatat cgccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga
    40261 cgggcagagg cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct
    40321 tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc
    40381 cacggcccca ccatctgatc gcgcgccacc atgccggtta cgctgcggtc accgatggtc
    40441 accaggaaag ttttctccgc cacggtcggc agatgcagta cgcgtttcac cgcgtctgca
    40501 atggtgatcc cttcacggac cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg
    40561 gtcatcttcg gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca
    40621 aaatgacgat cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag
    40681 ggtgcgcgct cacgcttaca cagttcgtca aacagcggta attgatcggc agcaaccgcc
    40741 agcacgtagc gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg
    40801 tcgcttagaa tatcgcgcag ttcaaattta ccgccgcgtc cgccgtcgct caccagctcc
    40861 ggcatcgcgt tagaaagacc gcccgcaccg acgtcgtgga taaacagaat tgggttagca
    40921 tcaccaagct gccagcaacg gtcgatcact tcctggcaac gacgctccat ctccgggttg
    40981 tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact gaccagacgc catggaagac
    41041 gccgcgccgc cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagcttcgca
    41101 ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc
    41161 agcatgatcg gtttgtgata accgcgcagc tcttcgccat tgtggctgtt cactttttct
    41221 tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa attcgttgtt aaacgccgca
    41281 ccgcccagcg ggccttcggt catgatgtcc agcgcggtaa caatgcgctc aggcttaccg
    41341 aaatcttctt cccacggctg ttcgaagcca ggaattcgca ggttggatac ggagaaacca
    41401 accaaaccag ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg
    41461 ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg
    41521 actttcatca ggatgtgcgc cggttcctga tggaaattgt agcggcccgt ttcgtggtca
    41581 gcaaagtagc ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag
    41641 agaacgtgat ctggcgtggt ttcgaaagta tttttgatca tcctgaacag cgatttcggc
    41701 tgttgttcac cgtcgataat ccagtcggcg ttaaaaattt tgtggcggca gtgctcagag
    41761 ttcgcctgag cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa
    41821 gcatcctgca gatagtcaat ttcatcttcc gccagagcca agccaagacg caggttagcg
    41881 tcgatcagcg cctgacggcc ctgccccagc aaatcaacgc ttgtgaccgg agtcggttga
    41941 tggtgggcaa acaactgctc tgcatcatct aaatcaaaaa agaccgtttc catcatgcgg
    42001 tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata
    42061 tagtaagcaa cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgggcaata
    42121 tcggtcgctt tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt
    42181 ttgccttgcg gggcgtggct ggcgagtgcc ggaccatatt tcagcaggcg ttcaagttgt
    42241 gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa
    42301 atattgtgaa ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat
    42361 gccgacagtg caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt
    42421 tcggtgtacc caaggggggg ggaacgggcg tcattataaa gaatctgatg cgctgacgaa
    42481 accgtttgcg tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggctg
    42541 taataagttg ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt
    42601 tcactattat tacgtttttt caagctggga cgcgcacgac acagagaatt aactaattga
    42661 aaaaattaaa gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc
    42721 tctggccatc cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc
    42781 ggggagagtt gcgtgtgagc accattcata ctcccctgac ttataacgaa atcaacggga
    42841 aaccttttgg cctggattac gaactggcga aacagtttgc cgattacctc ggcgtaaaac
    42901 tgaaagtgac cgtgcggcag aatatcagcc agctgtttga cgaccttgat aatggtaacg
    42961 ccgacctgct ggcggcagga ctggtctata acagtgagcg ggtaaaaaat tatcagcctg
    43021 gccctaccta ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccac
    43081 gtacgctggg caacctgacg gcggagcaac tcaccgttgc accgggtcat gtggtagtta
    43141 acgatctcca gaccctgaaa gaaacaaaat tcccggaatt aagctggaag gtagacgaca
    43201 aaaaaggctc tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg
    43261 ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg
    43321 atatcaccga tgaacaaccg gtgacttggt ttagcccgtt agatggcgat aatacccttt
    43381 ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg
    43441 aagagaaata cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac
    43501 gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa
    43561 ttgactggcg tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca
    43621 cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg
    43681 gcattacgga tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg
    43741 atatgatgag taaagtgccg gaaagtgtgc cggagaacga acggatctgg tttgccctcg
    43801 ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca
    43861 aagggaatcc tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac
    43921 cctattacag caagctgact tacggctacg ctcgtgggca tgaagcctac gcttatgtcg
    43981 aaaatattcg taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg
    44041 ctacagaagc ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg
    44101 gcaaagagaa atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc
    44161 attctccctc tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg
    44221 aggattgcgc ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga
    44281 gcaacgccgc gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac
    44341 ccggatgatg cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac
    44401 caaagaccac gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt
    44461 caagcgtgac atacaacgtg gcgtcgatca gacgataatt ttgcatcacc agaccaccct
    44521 gccgcagggc catgatttct gcatgtgcgg tgggatcatg gcgaccaatc gggcggttcc
    44581 agccttcgcc gattacccga ttgttatgca ctaataccgc gccgaccggc acttcccgct
    44641 catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat ccaatattcg tggctaaatt
    44701 cgacttcaga caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct
    44761 atgctttact ctattccagt tgctggagtt caccgcgcgg ggttactcgc caacgatgct
    44821 gacagaaata aagcagcgga ttgtcctgat tactgtcgct atagccactg tacagccgca
    44881 gcggcgtgcc gattttgcgc tccagttgtg cgaccttttc atgtcccaga caacgcatcg
    44941 tcaataccca accaccatag ccacgctgaa tttggctggc gataagatta acccgcggca
    45001 gccagggcgt atcgaaataa accgcttcaa ccagcggctg cggagagccg gtaatcaacc
    45061 agatatcagc atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg
    45121 cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt aaacgtgctt
    45181 cgctgtgacc aaaagtgcac ccccacagaa gcagactcat cggccagcgt gccgcacgac
    45241 cttttatcaa taacgcaatg gctataatcg gtaacaacgg caggacaagt aacgcattca
    45301 gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg
    45361 ttccatctaa gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg
    45421 gactgaaaca tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt
    45481 tccggcaaca atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg
    45541 caatggaata taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca
    45601 tcgcgaggaa agatatgaac ggcttacttc gtatccgtca gcgttaccaa gggcttgccc
    45661 agagtgataa aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa
    45721 gctctcagca actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc
    45781 aaaaactcgg ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa
    45841 gccagccgga atcgccctcc gtgcccattc ataaccaaat ccgcggtgat gatccgttac
    45901 ggctggtcgg cgaaaaactg attaaagaaa ataccgccgc tatgtacgca acgctaaacg
    45961 ttaatagtga agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa
    46021 ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga
    46081 agattggctt caatgctgcc gcactgcgcg atatgcatgc gctactcgca acagtacagg
    46141 cgtcgtcccc tgacgatctg ttattagcca tttcctacac cggtgtacga cgcgagttaa
    46201 acctggcggc agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta
    46261 ctccgaatgc cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag
    46321 cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg gatttgctgt
    46381 tcattgcgct gattcagcag gatctggaac tggcaccaga acgtattcgt catagtgaag
    46441 cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt
    46501 tgttttgaga gtttccttat ggcgctgtta atcactaaaa aatgcatcaa ttgtgatatg
    46561 tgtgaaatca aataaaaaca catttatttt catagtgata cagcaatgtg ttattgaatg
    46621 tgttgttgga tttatggtgc aaaaaaattt tgcataagtt cagcaacaca aagaacacca
    46681 ctttctttaa cgtcctccgc gccctaaagg acggggcttt acggcgcaaa aggcctgtga
    46741 catacaacat taacttttca gtcaaccgta gcacgttctt gtatacgccg tgtctgcggt
    46801 tttatataac cctggctgga aacctcttat acaaagtcga ttcgccaaca tcatagatga
    46861 tcgccacctt ctggcgagaa actcctgatg caattagtcg cccggcctgc gcccattatt
    46921 ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg tgcgcgagca gcttccagtc
    46981 ctgcttttgt tcgttcaaca atcagttcac gttccatttc agccagggca cccatcacat
    47041 ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga atcagtcaga ctacgaaagt
    47101 tgatgcctcg ttcgcgcaac gcctccacca gcacgacaag atgccgcata ctgcgcccca
    47161 gccgatccag cttccagaca accagtgtgt cgcctgccga taatgtcttg agcagttttt
    47221 tcagccccgg tctttcggac tttgtaccac ttatcttgtc ttcaaaaatc agctcgcatc
    47281 ctgcacagtt caacgcatta cgttgtagat c
//
LOCUS       DADLEE010000022        46417 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000022 DADLEE010000000
VERSION     DADLEE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46417
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.022"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(133..208)
                     /locus_tag="JQH51_001310"
     tRNA            complement(133..208)
                     /locus_tag="JQH51_001310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Lys,seq:ttt)
     gene            complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="JQH51_001311"
     CDS             complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="JQH51_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY6075711.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1174..1695)
                     /gene="pal"
                     /locus_tag="JQH51_001312"
     CDS             complement(1174..1695)
                     /gene="pal"
                     /locus_tag="JQH51_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY6075712.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="JQH51_001313"
     CDS             complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="JQH51_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY6075713.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="JQH51_001314"
     CDS             complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="JQH51_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY6075714.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="JQH51_001315"
     CDS             complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="JQH51_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY6075715.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="JQH51_001316"
     CDS             complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="JQH51_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY6075716.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="JQH51_001317"
     CDS             complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="JQH51_001317"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY6075717.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="JQH51_001318"
     CDS             complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="JQH51_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY6075718.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="JQH51_001319"
     CDS             complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="JQH51_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY6075719.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="JQH51_001320"
     CDS             complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="JQH51_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY6075720.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="JQH51_001321"
     CDS             complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="JQH51_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY6075721.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="JQH51_001322"
     CDS             complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="JQH51_001322"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY6075722.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12780..14756)
                     /gene="mngA"
                     /locus_tag="JQH51_001323"
     CDS             complement(12780..14756)
                     /gene="mngA"
                     /locus_tag="JQH51_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY6075723.1"
                     /translation="MVLFCRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTLQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14866..15588
                     /gene="mngR"
                     /locus_tag="JQH51_001324"
     CDS             14866..15588
                     /gene="mngR"
                     /locus_tag="JQH51_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY6075724.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="JQH51_001325"
     CDS             complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="JQH51_001325"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY6075725.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="JQH51_001326"
     CDS             complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="JQH51_001326"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY6075726.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="JQH51_001327"
     CDS             complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="JQH51_001327"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY6075727.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="JQH51_001328"
     CDS             complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="JQH51_001328"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY6075728.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPAPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22154..22870)
                     /locus_tag="JQH51_001329"
     CDS             complement(22154..22870)
                     /locus_tag="JQH51_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY6075729.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="JQH51_001330"
     CDS             complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="JQH51_001330"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY6075730.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="JQH51_001331"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="JQH51_001331"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY6075731.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="JQH51_001332"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="JQH51_001332"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY6075732.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25778..25885
                     /locus_tag="JQH51_001333"
     CDS             25778..25885
                     /locus_tag="JQH51_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075733.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26091..27374
                     /gene="gltA"
                     /locus_tag="JQH51_001334"
     CDS             26091..27374
                     /gene="gltA"
                     /locus_tag="JQH51_001334"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY6075734.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27765..28331
                     /locus_tag="JQH51_001335"
     CDS             27765..28331
                     /locus_tag="JQH51_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6075735.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28382..30841
                     /locus_tag="JQH51_001336"
     CDS             28382..30841
                     /locus_tag="JQH51_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6075736.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30856..31587
                     /locus_tag="JQH51_001337"
     CDS             30856..31587
                     /locus_tag="JQH51_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY6075737.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31584..32660
                     /locus_tag="JQH51_001338"
                     /pseudo
     CDS             31584..32660
                     /locus_tag="JQH51_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32767..33858
                     /locus_tag="JQH51_001339"
     CDS             32767..33858
                     /locus_tag="JQH51_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY6075738.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33855..34646)
                     /gene="nei"
                     /locus_tag="JQH51_001340"
     CDS             complement(33855..34646)
                     /gene="nei"
                     /locus_tag="JQH51_001340"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY6075739.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34682..35416)
                     /gene="pxpA"
                     /locus_tag="JQH51_001341"
     CDS             complement(34682..35416)
                     /gene="pxpA"
                     /locus_tag="JQH51_001341"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY6075740.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35406..36338)
                     /gene="pxpC"
                     /locus_tag="JQH51_001342"
                     /pseudo
     CDS             complement(35406..36338)
                     /gene="pxpC"
                     /locus_tag="JQH51_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36332..36988)
                     /gene="pxpB"
                     /locus_tag="JQH51_001343"
     CDS             complement(36332..36988)
                     /gene="pxpB"
                     /locus_tag="JQH51_001343"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY6075741.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37011..37754)
                     /gene="ybgI"
                     /locus_tag="JQH51_001344"
     CDS             complement(37011..37754)
                     /gene="ybgI"
                     /locus_tag="JQH51_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY6075742.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38025..39506
                     /gene="dtpD"
                     /locus_tag="JQH51_001345"
     CDS             38025..39506
                     /gene="dtpD"
                     /locus_tag="JQH51_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY6075743.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39656..41074)
                     /gene="phrB"
                     /locus_tag="JQH51_001346"
     CDS             complement(39656..41074)
                     /gene="phrB"
                     /locus_tag="JQH51_001346"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY6075744.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41071..41580)
                     /locus_tag="JQH51_001347"
     CDS             complement(41071..41580)
                     /locus_tag="JQH51_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY6075745.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41709..41996
                     /locus_tag="JQH51_001348"
     CDS             41709..41996
                     /locus_tag="JQH51_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY6075746.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42051..42431)
                     /locus_tag="JQH51_001349"
     CDS             complement(42051..42431)
                     /locus_tag="JQH51_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075747.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42434..43069)
                     /locus_tag="JQH51_001350"
     CDS             complement(42434..43069)
                     /locus_tag="JQH51_001350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY6075748.1"
                     /translation="MTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRET
                     GLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKAN
                     RLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGS
                     YHWDDIKDPSVTKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(43014..45820)
                     /locus_tag="JQH51_001351"
                     /pseudo
     CDS             complement(43014..45820)
                     /locus_tag="JQH51_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(45839..46060)
                     /locus_tag="JQH51_001352"
     CDS             complement(45839..46060)
                     /locus_tag="JQH51_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY6075749.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
BASE COUNT        11165 a        12629 c        11200 g        11423 t
ORIGIN      
        1 actacgctgg atactacaac taactatgtt agtgggggtc gtgcaggatg actcgacttc
       61 gtctcgccct tcggcgccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc
      121 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      181 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
      241 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
      301 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
      361 gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc
      421 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
      481 ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt
      541 tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa
      601 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
      661 attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc
      721 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
      781 agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt tgatcgctgc tggttgattg
      841 cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc
      901 cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc
      961 ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga
     1021 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagctgacac tactgattgg
     1081 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
     1141 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
     1201 tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
     1261 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
     1321 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
     1381 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
     1441 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
     1501 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
     1561 cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt
     1621 gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
     1681 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
     1741 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
     1801 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
     1861 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
     1921 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
     1981 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
     2041 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct
     2101 ggtcagaagt aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac
     2161 tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
     2221 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
     2281 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa
     2341 taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
     2401 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
     2461 catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg
     2521 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
     2581 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
     2641 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
     2701 gattcggagt gacttgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg
     2761 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
     2821 atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac
     2881 caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt
     2941 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
     3001 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
     3061 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
     3121 ctgatgttga ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
     3181 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
     3241 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
     3301 taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
     3361 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
     3421 ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg
     3481 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
     3541 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc gctgccgctt
     3601 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttatcagcag
     3661 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
     3721 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt tcagcagctt
     3781 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
     3841 tcagtgccgc agcggctacc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
     3901 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgctgcttct tccgcagctt
     3961 tagcatctgc cgccgctttc gctgccgcca cttcagcttg cttctgcttt aactcggcct
     4021 gttttgcggc ttcttcagcc tgctttttct gctcctgagc cgctaaccgc tctttctcaa
     4081 gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca gcagcctgct
     4141 gttccttcat cttgcgctgt tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc
     4201 gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg gacgaaccac
     4261 cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc agcgccgcaa
     4321 ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt
     4381 cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg ctctctttca
     4441 agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga tttcacaccc
     4501 gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc ttttgcgcca
     4561 ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc cgccaccacc
     4621 tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta ctgaccaata
     4681 ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg tgattcagta
     4741 gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc
     4801 agcaacagca ccagcagtac gtccagcaac ggaacaatgt tgatttcgga cttgagatcg
     4861 cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc gctaacggta
     4921 aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta attcagttcc
     4981 agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat tgcggcgaac
     5041 agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt
     5101 gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac cgtaccaaac
     5161 agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg cgtttccaga
     5221 ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc cactacggct
     5281 tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc gctgtaaaag
     5341 atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata gaggcgagag
     5401 agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc cgctgcgtta
     5461 agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc aatcaaaatc
     5521 aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat attcatgtca
     5581 gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc attttgagtg
     5641 ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg gcgttgacaa
     5701 tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc tgtatttcga
     5761 gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt accacaaagg
     5821 caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc atctctgtgc
     5881 gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg gcatcggtat
     5941 cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta catcccggta
     6001 atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt gaggtttggg
     6061 aatgagggca agttaagggc gcgtaaaaat ttatgggcct agagcggccc ataatttttt
     6121 aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa caatatcggc
     6181 aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg
     6241 aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt ccagttcact
     6301 ggtcttcgcg gcaaaacgag atgggtccca aaacatacat cctgctaaca gaagcgccat
     6361 cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg tcgcgataat
     6421 cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc
     6481 gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc gaaataccac
     6541 atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct tctttggtga
     6601 tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc ggtaccagaa
     6661 ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca tcccacattg
     6721 tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt gcgataccgg
     6781 ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac gcggctttat
     6841 ccatacgtgc agtcaggatg gtcagcagca gcagaaccac acccagtgcc ggaatagccc
     6901 acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga accacttctt
     6961 tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca taaccatcga
     7021 taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc agcgcagcca
     7081 cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg
     7141 ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg ccgaacgggt
     7201 taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca acgttgaacg
     7261 gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga acgaagctac
     7321 caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc ttggagcggt
     7381 agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc gccacataga
     7441 agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg
     7501 tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc ataattcgac
     7561 gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt
     7621 cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata aaacgcaata
     7681 cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta gtcgtggaag
     7741 actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt gcaaacttga
     7801 acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc agcaccattg
     7861 agaagatgag atcgcctgcg gtcagtgacg aattcgccac agctgtcggc agcacttcac
     7921 cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct acagcaatcc
     7981 acggcagcgg aataccgtac agcgcggcgc gcagaagcca ttttttctcg ccaatgcggt
     8041 tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag ccacacgcca
     8101 ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg gatggagtct ttggttgcct
     8161 gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc agcagcagac
     8221 cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgtctgg tcggtagaac
     8281 cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg cgttcttcat
     8341 gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc gttgcaatga
     8401 tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa
     8461 tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc ccactcggct tcaatagcag
     8521 ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc agaacaataa
     8581 cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg
     8641 cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac atcgcgccag
     8701 tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg ttaagcacca
     8761 gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg gacgcgattg
     8821 ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg
     8881 ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa ccgaagaaga
     8941 acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt gccagcggcg
     9001 caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg aactggaact
     9061 ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac ttgccccaga
     9121 acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt tccataatgg
     9181 ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca
     9241 aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct tgctcatcgc
     9301 atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc aatagaaatt
     9361 tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg
     9421 caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc aaaatcctta
     9481 caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca atttatagct
     9541 aaattgccgc ctttcagcca atttgatcga gaacaattta tctctttttg atgcccattt
     9601 ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca tgtttcaaca
     9661 aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt ataagactga aattagtttc
     9721 ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac cttaattctt
     9781 tttttggtca ctggctaata cgacgcagag tttcaggatt attaataaag gcataaatct
     9841 ccctcccgcc gatattaaca tttaattaac ttataattat attataatta actcacccgt
     9901 ggttattatt ttgttaaaaa taaagccaca tatacgtagc ttgagagtga agataaacac
     9961 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10021 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10081 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10141 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10201 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10261 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10321 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10381 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10441 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    10501 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    10561 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcctaggcc ttaaaagcag
    10621 atcttctttc cccagtaagc ccacgccgcg cagcaacgta atggcaaagg ttttcttctc
    10681 ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag acagccatgc cgttacgccc
    10741 ttcctgtaag gctgcatagt tgagcatatt ccataccgga accggcgctt ctttccagct
    10801 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    10861 ggtatctgcc agaacactat cggtgttaaa agatgtaggg atcaggacgc gaaggcgatg
    10921 atcgtcagcc tggttatcaa ggttgatatc cacatcaatg cgcctgttgt tatgactaag
    10981 agtgacaacc aactcgacat ctactctgcc agtggattgt cttgcgctgc gttgttgcag
    11041 attacgcggc accgccattt catagcggat aacagccctg ctctgccagg cctcatgaat
    11101 aatctcgcat tgcggtttcg cattcgccga agtaattacc cactcttctt ttgcgggtga
    11161 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaacaccc ggtcatagcg
    11221 cacaccgcta tctttatcta ccagttgtag cgttccatcc tcattgagcg caatttgcca
    11281 gaaagcattt tccagtatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11341 attcgcttcg atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgtat
    11401 atcaaactcc ataaagggat cgtaattacc gtaatgaact atttgccgat cgattaggcc
    11461 tggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtctcg
    11521 tagattaaac tggctggcgc gcagccgcac agtggtgttg atcacttctt cccgcggcca
    11581 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtcggcgat
    11641 tttacgcata tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    11701 gacgatttcg cgatgaactt tgtcacgaca gcagcagccg atgctgtcgt gggcatgatt
    11761 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtatccaa aacccaacgt
    11821 cctggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    11881 ggcaactttg atatccatgc gcgtggaacc gatggtgcga tgcacgcgca tatatttgcc
    11941 atcaataaat tcccctttca gggttgccag attatctcgc tgcgcttcga tcttctcaaa
    12001 tacctcttca aagcggctca tcacaaactt tcgttgagga taaatttcgc gcagtttctc
    12061 catcacttcg aagatatttt gctgcaatgg catctgatca tgcccattcg gcagcaaaat
    12121 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttgcgtaa
    12181 tccgttttcg tccgcaggta agtacttacc gatggcataa cccaacggca tcacctgcgc
    12241 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatccg taccgtggcg
    12301 ctcagaacat ccacgccaga acatggtgca ggtaatgcca aatccattgt agatatgcgg
    12361 cagttgcccg gacatgccaa aggaatcggg taaataacct attttcatcg gctcgccaaa
    12421 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    12481 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttgccggctt ctaccagttt
    12541 cttcaagcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    12601 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    12661 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    12721 gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    12781 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    12841 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    12901 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccagcaccg
    12961 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13021 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13081 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13141 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13201 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13261 gcggctttat tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13321 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13381 caggcgacag gttcccccac cacaaacagc atcagactgc ccgctcccaa tgtaccgagc
    13441 accgggtaga gataaaaagt caggaagcca ttgaatttac tgctaagacg caagtgattt
    13501 ttcatccagc gtatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    13561 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    13621 gccagcgaat aggcggtata ggctgccagc accggtacca tcaaaattcc gagcagcccg
    13681 ccgcccagtt tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    13741 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tcccccccgc gacaatcaac
    13801 ggtacggcaa aagagatccc gctcaacagt gcctgtttca gctccgtttt gacgcttttc
    13861 accggttgcg tatcttgctg taaggtacgc gtctcatcgc cacgctcgag ggtaagcgat
    13921 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcacaga aagcgcagga
    13981 atgccgttaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcagtt
    14041 gcactattga gttgatccgc cgttaaacgc ccttcaatac cgttagcacc ttgcttttca
    14101 acgtagacat tcacgccgag tttgcgtccg gctttttcca gatactccgc agccatgtag
    14161 gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga tagttggcgc gttggaaaaa
    14221 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaattc atcaggcgtc
    14281 gtcgccgact gtatacgcgc ccgaatctca tcatccgcca ggcgcgtggt cagcgctgtc
    14341 agcagttgca tgtgcgtagt acccgcttca ttgaggggaa tcgccagcaa caccactaaa
    14401 tcaaccgctt ccgggccatc cacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    14461 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcactt
    14521 aaggcggtcg ggccaaggct ttcacggcga tacacttctt tcagaaattg ctcagtactg
    14581 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt gaatggcctc ttcacggctg
    14641 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag gttcatcatg
    14701 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gacaaaacaa taccattgcg
    14761 taatcaattt cacttgattg aaattatttg cattttattt gtattgttta caggatgagt
    14821 ttatcgggca taatggctaa attcgtatta atgagatgct aaataatggg acacaaaccc
    14881 ttataccgac agattgctga tcgcattcgt gagcaaattg cgcgtggcga gcttaaacct
    14941 ggcgatgcgt tacctaccga atcggcatta caaacagagt ttggcgtcag ccgggttacg
    15001 gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc tcgaaagcat tcagggcagc
    15061 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15121 gaaaaactgt cggaccgtca cgtcgataca cacagtgaag ttctgatatt tgaagtgatt
    15181 ccggctgacg attttcttca gcaacagcta caaatcaccg tgcaggatcg tgtttggcat
    15241 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15301 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15361 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15421 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15481 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15541 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaggc
    15601 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    15661 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    15721 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    15781 atccgcagtc cctttcccac cggcaatgat cgcacccgcg tggcccatac gtttgccttt
    15841 cggcgcagtc acaccagcga tgtaacccac aactggcttg gtaacatgct ctttgatgta
    15901 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    15961 ctgcggatct ttttcgaaca tttcgagaat gtcgataaag ttagagccag ggatcgggtc
    16021 accgccgata ccgacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16081 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16141 acccggctgg ataccaattt tgcattcacc cggggtgata acgcctgggc agttcgggcc
    16201 gatcatacga acgcctgctt cgtccagttt cactttcacg gtcagcatat ccagcgtcgg
    16261 gatgccttca gtgatggtga taatcagttt gatgcctgca tcgatagctt ccagaatgga
    16321 gtctttgcag aacggtgctg gtacgtaaat aacagacgcg gaagcgccag tagcagcaac
    16381 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtgccgc ctttacctgg
    16441 agttacgcca ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    16501 ctggctaccg gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    16561 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    16621 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    16681 agacgtacca cgaccggtac gttaacaccc acttccgcta ccgcgccgat gataccgtca
    16741 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    16801 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    16861 acgtccagga agttagccgg ttcgccgccg tgcagtttaa ctatgtccat cgtacccatc
    16921 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    16981 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17041 agatcaggct ggcggaacag ggcgttgcca tcagcgccca gtttgccgtc gaggcaaatc
    17101 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcagcgccag gtcgcgctcc
    17161 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17221 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17281 agcgcaactt tatggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17341 ccttcggtgg aggccataaa gaccacgcga cgggaactac ggtctacaac ggcaccgaga
    17401 tacagctctt tagcgatatc ggtcgccgct tcaaccagaa tctggttaac cggttggcca
    17461 ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc aaaagcacgg
    17521 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    17581 acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc ttctgcttcg
    17641 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaacagttgt
    17701 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    17761 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    17821 tacacgtcca gcagcagacg cgtcggctct tccagtaact ctttgatcgt taccaggaag
    17881 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    17941 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18001 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18061 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccatcacgg
    18121 cctttgactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18181 tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa ccgccatgct gacgtcgaaa
    18241 tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt tcacttccgg gtaacgtttc
    18301 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18361 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18421 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    18481 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    18541 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    18601 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    18661 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    18721 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    18781 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    18841 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    18901 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    18961 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19021 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19081 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19141 ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19201 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19261 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19321 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19381 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19441 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    19501 gatgacgcag cagggatttc ggcgacatca cgaccagcgg acgacgcatc ccgcgcagcg
    19561 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggtacgcaa acctgcatgt
    19621 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    19681 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgct
    19741 cgccagagga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    19801 gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc ataaccatat tcaaacgcca
    19861 gcacggcttc ttctgacagt acggagtccc agacacggaa cgcgccctga ccgttatgga
    19921 tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    19981 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcatcaa
    20041 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggctg
    20101 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttctg
    20161 gtaccgtact gatacgttta gccagttcct gcaggcgctt catctcaact ttgttcgggt
    20221 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20281 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20341 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20401 tgcgcggtgc cggatgtttt ttgattttct gatacatcag tggctgggtt gcgctcggct
    20461 cgtcggcttc gttgtggccg tgacggcggt agcataccag gtcgatgaag acatcacgtt
    20521 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    20581 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    20641 tagaacgggc atccagcggg ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    20701 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    20761 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgt
    20821 tcggctcatc aagtctgtcc agacgggcac gaacagagcc gataacgacc gggcttacaa
    20881 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    20941 agtcagacga gaagcccatg tggtatttca cgtcgcctgt gccgaggtgt tctttatgtt
    21001 taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21061 tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21121 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21181 ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc ttcagcggcg gtcagttcgc
    21241 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21301 ggatccagcg tttttcttcg gtgctggtga tgtgcatata ctcggcacca atcgggccgc
    21361 agtaggtttg cttgagggcg gaaataagct cgccgagttt catggtttct ttgccgctgg
    21421 caaatgaacc gacgttgaag gtttcctgga agtctgcttc ggtcagatcg tggaaagacg
    21481 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatctaga ttcgcatgct
    21541 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    21601 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    21661 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    21721 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    21781 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    21841 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    21901 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta cgtagtggct
    21961 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22021 tttctttgtt acgcctgatg cgctgcgctt atcaggcctg cgggtaatga cttgtaggcc
    22081 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcaacgctt gcgcgtctta
    22141 tcaggcctac agtttacgca ttacgttgca acaacatcga cttgatatgg ccgatggcgc
    22201 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22261 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22321 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22381 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22441 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    22501 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    22561 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    22621 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    22681 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    22741 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    22801 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    22861 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    22921 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactca ggcagataca
    22981 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23041 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23101 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23161 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23221 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacggatcg
    23281 ccaccggatc ttcaccgtta cgattattgt tccaccggtt caggcgatcc agagacgctt
    23341 caacatcaga ctcgctggca tcgtgcagtg cgccctgctc ggcgatagac tcttgcagat
    23401 gcagacctgc cgcgcgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    23461 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagtccc ggaacaacca
    23521 catcttcgcc tttctcattc acagtcagcg cctgaccggt aactttggtc ggaataccgc
    23581 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    23641 cgaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    23701 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    23761 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    23821 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    23881 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    23941 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24001 taatgtgggc gttagtggtg gactgataaa tacgccctgc tccgccagtc gccagcacgg
    24061 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24121 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24181 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24241 cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24301 gacgttgata gatacgacca tcatcgagac gcgagaacgg caggcccatg tgttcgagtt
    24361 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24421 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    24481 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    24541 tagagagcag ggcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    24601 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    24661 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    24721 cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    24781 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    24841 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    24901 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    24961 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25021 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25081 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25141 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25201 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccactg caggatgccc
    25261 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25321 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25381 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25441 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    25501 cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    25561 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    25621 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    25681 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    25741 acaaagttgt tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    25801 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    25861 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    25921 ttcgtcatca atggattctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    25981 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26041 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26101 caaaagcaaa actcaacctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26161 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26221 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26281 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26341 tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26401 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
    26461 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    26521 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
    26581 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    26641 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    26701 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    26761 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    26821 cttcgggggc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    26881 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    26941 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27001 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27061 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27121 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27181 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27241 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agttacggta
    27301 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27361 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27421 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
    27481 attcatatat tctaaaaaaa aatacatcgg tataaattat aaacaaatag atgattttta
    27541 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagcgca cccctataat
    27601 tcccaatact ttattcatca aacatttaat tcataccatc aattaattac ttttacattt
    27661 aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa ataagatatt
    27721 cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa
    27781 aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg
    27841 aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc
    27901 aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt
    27961 cacatcacag cgatgccgtt gctgtcgacc tgctcttagt caactgtgat ctggaaaact
    28021 ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga
    28081 aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg
    28141 gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata
    28201 tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa
    28261 ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg
    28321 attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg
    28381 tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc
    28441 ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat aaatcaatgc gcgaccgcat
    28501 tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt
    28561 tgcggtaaat aataaccaaa tcagtaacga gcaaaagatt aactggcaca aaaatgacga
    28621 taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt
    28681 ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct
    28741 cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc
    28801 gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtgtcct
    28861 gatggattac aacttgtttg ccagcagcta ccgcccacag gatggcagca gcagcactaa
    28921 tcttaacgcc tatggaacag ccggaatcaa cacgggggca tggcgcttac gcagtgatta
    28981 tcagttgaat catactgata gcgatgataa ccatgaacag tcaggcgaaa tatcacgtac
    29041 ctatcttttt cgcccgttac cgcaattagg ttcaaaatta accctcggcg aaacggattt
    29101 tagtcccaat attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgatcg
    29161 aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa
    29221 tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag tcccaccagg
    29281 cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac
    29341 ggaagaagat ggtcgggtga acaatttcca ggtttcagca gcatcgacgc ccttcctgac
    29401 tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca
    29461 tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac
    29521 ctcgctgtac agcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat
    29581 tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca
    29641 ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt
    29701 ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca
    29761 cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca
    29821 gacgatcagc ttatctgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa
    29881 cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg
    29941 ttttaatgtt gatattggtg actggagaga tatctctatt tctacgtcat tcaatacaac
    30001 ccattacgaa gataaagatc gcgacaacca gatttacctg tcgatttcgc tccccttcgg
    30061 taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat
    30121 gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc
    30181 cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg
    30241 tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg
    30301 gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa
    30361 tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct
    30421 cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc
    30481 caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat
    30541 caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa
    30601 agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat
    30661 ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt
    30721 aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc
    30781 gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta
    30841 attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt
    30901 ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat
    30961 aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc
    31021 tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata
    31081 caacgtctgg aaccgaaagc aacaacacag gtacggatag taaagcaagc ctcgaccaca
    31141 aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc
    31201 ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg
    31261 ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag
    31321 gtcagccagc agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt
    31381 gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg
    31441 ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt
    31501 tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat
    31561 ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc
    31621 tgttatttct gtcgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg
    31681 gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg
    31741 gcgataagat ctgggaatct gacgatatta aaataagatc tgggaatctg acgatattaa
    31801 aattcccgtc tattgtgaca acaataccaa cggcaacttt gaaagcgaac atgtctacgt
    31861 atgggtaaac ccctacccgg gggtgcagga tcgttattat caactcggag taacttacaa
    31921 tggcgttgat tatgacgcca accagggaaa aagccgcatc gacactaacc agtgtatcga
    31981 cagtaagaac attgacattt atacccctga tgggatggca gaacaaaatt tgctcgggtg
    32041 atcccgctaa tattcacatg tcgcgaacct ttctcgctcg catgcggtta tacgtcaaaa
    32101 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
    32161 ttgacggtgc cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta
    32221 ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
    32281 cacagatgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
    32341 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
    32401 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
    32461 gtaatggtct gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat
    32521 atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacagctg
    32581 aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt
    32641 ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt
    32701 tcagaaaatt gctgattttc cccttttcct tgattgctgt tattttttcc tagcccggct
    32761 ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt
    32821 ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta
    32881 catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc
    32941 ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc
    33001 atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc
    33061 gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg
    33121 cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg
    33181 gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggtggc gtttatgcaa
    33241 tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt
    33301 gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc
    33361 ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct
    33421 tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc
    33481 accatcgaac taccggaatg gctgctggcg atggcgtata tggcaattgg ctggcggatt
    33541 ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg
    33601 ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg cgtggggact gacccggttt
    33661 atgcatattg attttatgac tgcctacctc gccaccagtc ccggcgggct tgataccgtg
    33721 gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg
    33781 cgcctgttca gtattttgct gacggggcca gccattgcgc ggtttatttc aacctatgcg
    33841 ccgaagcggt cggcctagtg ctggcagcct ggacaccagt aaaacgggcg cgaggacaac
    33901 gtggttttct caatgatgcc gccacagcgt tcgcacagtt cgccatcacg atgaaaaacc
    33961 ttaaagcgaa acagcgcccc atgatgctta ttctcatcca cctgcccccg cgtagcgtaa
    34021 gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga
    34081 tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa
    34141 ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg acggttacga
    34201 aagcgcggcg acaataaccg ttcctttacc acctccggcg tcagattcgg atccagcaca
    34261 tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct caacatctca
    34321 atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat
    34381 actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga
    34441 ttatggctgt agagcgttaa accgttggga aaatgagtta acaacgcctt accacgcgtt
    34501 tccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag
    34561 gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca
    34621 cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt attaagcggg
    34681 gttatgctgc gacaacaatc ccctttttgg caaatgtagc tcgtaatcgg cgggcgaacg
    34741 ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg cgccgcgacc gttgcccatt
    34801 cgccggtgat acttttgact ctgccgtgtt gcaccatctc cagcgtttgc gccaacgcct
    34861 gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg caccagcgag ccgtcagcct
    34921 gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac
    34981 gaatcagctc gcttcctgcc agcccaacga gaaccaatgc cggatcgcaa gcgtataccg
    35041 ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt
    35101 gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa
    35161 tttgatacag cgtctgggcg taaacggttt ccggcggcag ctgcatggcg ctgcgaccaa
    35221 aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt tttattgctt
    35281 cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg aaatccacaa gcaatattgg
    35341 cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt
    35401 tcaggtcaat tttcattgtg cagccgccac gctaattgtt caaaataacg ttgctgatct
    35461 tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc
    35521 ggaatttgcg ccagatggta catatcagcc tcaatgatac aggcaatacg tggataacca
    35581 ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgcgg cacctgcacc
    35641 acgcccggta acaaaccgtg agataacagt tcgcgatcgg tgatgcgttt taaaatttgc
    35701 ccctgtaaac gatagcccat tcggttactt tgcgagctaa gctgccaggg cgaacgccag
    35761 aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg
    35821 cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgatttg
    35881 ccaatcggga gtcagtcacc atccttcagt aaacggcctt ccagcccgcc aatccccact
    35941 ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc
    36001 agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc
    36061 ggcaatcgcc agccggtcca gacggcgtta tcatccaacc gcgcttcgca acctgcaccc
    36121 gtcagggcaa accacccatc agtttcgaac tcaactgtta actgaccgag cgtgatctcc
    36181 agcgcggggg cattggcgtc attacccacc agcaggttag caatgcgcag cgcaggcata
    36241 tccagtgcgc cacagtggct gatacccgac tggcaaaaac cgtgacgacc gccatcctgc
    36301 acagtggtgt acatgcccgc acgaataatc ttcagcatat tccctccttc tgcggtacaa
    36361 atcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc
    36421 tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag
    36481 tctgcggccc gccgatcccg acagaacctg ccggaacgag caagcgcggt tcagcgcgcc
    36541 gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta
    36601 aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctcaacc
    36661 cgcaatacgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa
    36721 tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac
    36781 gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgtg atattgttca
    36841 tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga
    36901 tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac
    36961 ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga
    37021 ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat
    37081 gcgcgaatac caccgcgttc agtggcatgg tgacctgcag cataaaaatg caatccctgc
    37141 tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca
    37201 aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg
    37261 accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc
    37321 caggaggcta gttccattcc cggcacaggc atggtcagct ctccccacgg caccaacggc
    37381 tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc
    37441 tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac
    37501 gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag
    37561 ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg
    37621 ctggcggtga caccggtaac aattttttgc accgtctctt tgccttccac ctgcaaacca
    37681 ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt
    37741 tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt
    37801 acatcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa
    37861 aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag
    37921 catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg
    37981 ccgcgcaact attacgtgac gttgccgtca attgtggaat tattatgaat aaacacgcat
    38041 cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt
    38101 acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc
    38161 acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg
    38221 gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga
    38281 tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt
    38341 ccctggcgat tatcgtctgc ggctatggcc tgtttaaatc taacgtaagc tgtctgctcg
    38401 gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcgg
    38461 cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca
    38521 gctgggcgat gggctttggc ctggcggcgg ttggaatgat cgcgggtctg gtcattttct
    38581 tatgtggcaa tcgtcatttc actcataccc gcggcgttaa caaaaaagta ctgcgtgcga
    38641 caaactttct cctgccgaac tggggatggc tgctggttct gctggtggca acgcctgcac
    38701 tgattaccgt gctgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta
    38761 ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcggaagg
    38821 agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac
    38881 agggcggtag ctcgattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt
    38941 ataccgttcc gaccgcgatg ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg
    39001 tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg
    39061 gaaaatttgc tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg
    39121 cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtgtta ggcctcgcgg
    39181 tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg
    39241 aaattccggg cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg
    39301 cgaactatct ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg
    39361 gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg
    39421 cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa
    39481 accgcgcgct ggcgctggag tcataatagg ccagcaccga tgcctgatgc gacgcttgac
    39541 gcgtcttatc aggcctacgt atttcctgca atttattgaa tttgcacaaa tttgtaggct
    39601 ggattaggcg ttcacgccgc atccggcatc tgggctcgat tgcctgacgc gtctgttatt
    39661 tccccttccg cgccgcctca tacgctgcca acgtttgtac tctcgcctct ttgtgctcga
    39721 ctatcggttg cggataatcc agcttcacac ctgctttctg cgcccacttc cacggctcat
    39781 gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca tcgacgaata aactcgccct
    39841 cacggtcaaa tttctcgccc tgagttatcg gattgaaaat acgaaaatac ggcgcagcat
    39901 cagtacccgt agaagccgcc cactgccagc caccgttatt agctgccaaa tcaccatcaa
    39961 tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca
    40021 gaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
    40081 gcatagcggc atcaataatc gggtatcccg ttttgccttt ctgccaggcc tgtaaatgtg
    40141 cgggattgct ttgccactgc acacgatccg tccaggcaat aaatggacga tgtttacaca
    40201 acgaggggta atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc
    40261 agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac
    40321 actggcgagg cgataacccg cccgttgcca agctggcgga caaacggctg gtgccttcca
    40381 ctgccggaaa atctcgttgt tgctcatact ctccagcacc gttctcgcaa aactggcgta
    40441 attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag
    40501 gataattcag cgtaatggat ggcgcgggct ttatcgatcc gctactacga acttttggtg
    40561 cagcgacgca ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg
    40621 taaagacttt gtacatctcg tgattgccgg tcatcaccgc ccccggcggc aggatcacgc
    40681 tgtcatcaaa tccttcacac accacgttac gcagcgctct ttcaacgtcc acatcccgcg
    40741 ctcgctcatt cacctcatac tgatagttat aaaaaagatg agtaacgctg ttttccgcgc
    40801 acacctgttt aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag
    40861 gaataccttt ttccgccagc gcgatttgta gcccattcag ttgagcattg ataagttcag
    40921 cctgacgcgg cgacatgtta tgcgtctccc acttgcgtgg tgtagcgata tacagcgcca
    40981 gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta
    41041 aatcctggcg aaaccaggcc agatgggtag tcataaaact cctgaatggc gcaagttaat
    41101 cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc
    41161 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
    41221 gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgcgggc gaaaataacc
    41281 ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag aaagcaaaac
    41341 aattacgcgt tggcggtact ggttgtaaaa atcatccaga ttttgccact gatgaatatc
    41401 agccacaaac gggccaagtt tgcggtactc gggctgagaa tgggccagaa agacgagttt
    41461 atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg
    41521 taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt gtagattcat
    41581 tactgctcct gaacggtata caggagtaag tgtaggcgtt ggtaaatgat gtttgtgaag
    41641 ttaatccgct agcagggcaa gattacgaaa gcgcgctccc cgcaagcaac taatcaaggt
    41701 agttaatcat ggctgaacac gatattacgc cagaagtcac tatttccaaa acacagcgcc
    41761 gctataaagt cggctatgtc agagcccgcc accaggaccg caaaaccggt atagcccggt
    41821 attattcaca acatcccagt ctgcacctta agggcgactg gctgaaagag gccggatttg
    41881 ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc atcgctgaca
    41941 gtgacgaggt gcaggaactg cgggatggga tgtttagtgt gctgaacgaa agttaaatat
    42001 taaaaaagct gggaagattt aaaaccttcc tagcttttta ttctgtctta ttataatgga
    42061 tactcatcat cttccgatga atttataaat tgctccaagt ttacatgaag agtacttaac
    42121 ctagttttta actcattaag tgaagcgata atttcatatt ttccaacata atcatatgat
    42181 ggatcgtatt cattatctat gtcagaagct aaaaatgatg atattcgtag tttaaattta
    42241 gcttcatctt tattacattc attggcataa ggtgaatgcc ctatcatagc atattcatct
    42301 aatctaaaag gataagatgg ttttaattca tcgatcaatg aaatttcaat ccatgttcta
    42361 tatccaggca ttaatggact aatttctttg agcaaaacct ttccacttaa taatgtattg
    42421 agattgttca ttattatgtc tcccacggga aaaatatatg tctaacactt ccaccatgtt
    42481 gatgaacttg taaatgtggt aaatcaccat caggatttcc tggcccatga cctgacactc
    42541 tatatgaagg ttttgttaca gaaggatctt ttatatcatc ccaatgatat gaacctgctt
    42601 ttgttttatt ttcagtaaga tattccatta agttcttagt gctggggcct gactcacctg
    42661 tcatattttt ataattatga cctaaatatc tatccataaa caattgttca gcgtcacttc
    42721 tagacctaac agtaactttg gtttcactac tatctaataa acgattcgct ttttggttac
    42781 taacagcaca tggagttaat ccaagtggat cagcatattt tagaggatta ggtgcataac
    42841 tgtagagatt taaaccgcct ctcaacccta tcgggtcagt taccatgaac cgcccgacat
    42901 ccggatcata gtacctgtgc agattgtagt gcagccccgt ttccctgtcg aggtactgtc
    42961 cctggtagcg caggttctgc tccaccccgc cgtggtcctt ctcctgtatc gtgttacccc
    43021 agacctgata ccccgtctcc cacacgattt tcccgtcgct gtccgtcatc tcttccggcg
    43081 cgccgttcac atccgtgtga aaatacagta tccggttcgg gccctctttg cccgccttat
    43141 ccacccgcgc cagcggttca taactcccct ggtccctgta gatgtacagg ctgcctgttc
    43201 cctccggcct gctctcctgc gccagccgca ttccgtccca taaaaatttt gtccggttgt
    43261 acggcttacc gtcagggctg atgtgctgtt tttccgtccg cctccccagt gcatcgtaca
    43321 cataccggta tgtcccccgc gctgtcgtca cttccaccat ccggtgttcc gcatcataac
    43381 ggtaactctg cgttccgctc cggccctcct tttccaccgc acggccaaat tcgtcataac
    43441 ggtagtgcac accacggtaa gacatcagcc ggttgaacgg caccacgctg ttttctgccg
    43501 tggccctttc ccccgcgcgc ctccccagca ggtttcccgc cgcgtccacc tccgtttccg
    43561 gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg tcgtgcgtct
    43621 ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga gggtcggtca
    43681 tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt ccggcttcgt
    43741 cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg cgccccagtc
    43801 cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac tgttcaccgc
    43861 tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca taccagcccc
    43921 actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag tggttttcgt
    43981 cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc aggtggccgg
    44041 cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa tgctgataaa
    44101 tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg cacggacgct
    44161 gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga ttcagacagt
    44221 tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc gggtcagcct
    44281 caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc tcaccacgcc
    44341 ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg gcttccatat
    44401 ccaagcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg ctacccggtg
    44461 tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt ccttccgtac
    44521 gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc ggcagactga
    44581 aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg accggatgcg
    44641 gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca gcaaaacagg
    44701 cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt gccaccaggg
    44761 ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg ccaatgcgca
    44821 catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc tcgcaggtac
    44881 agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc gcgccttccg
    44941 cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg atgctgccaa
    45001 taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc agcaggttaa
    45061 gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga aactgcgtca
    45121 gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg gatcccgggg
    45181 ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg ccatccacca
    45241 tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac atcagcacag
    45301 gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga tattccccgg
    45361 ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt tctgccttcg
    45421 cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct gcccgcgcgg
    45481 caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct tcaatctgag
    45541 gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct gtcacgccgg
    45601 tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg caccccatcg
    45661 tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca tcaataacga
    45721 catttgcggc cacctccagc accgccccca gcacatctgc cataaacgac gtatgcagca
    45781 gggcatcacc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc ctgtatgatt
    45841 aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt tcagcccagc
    45901 gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc atcatgaccc
    45961 gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta ccccggagaa
    46021 actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa tgtaaatagt
    46081 ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat gaatgtaata
    46141 cccatcaccg ctttacagca cactttatct ttccagtcat aatcactaat aatattaagc
    46201 aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa caatcccaat
    46261 atattcacta tgcaaataaa accactagta acagacacgc taccgcctcc atataaaaat
    46321 ggacacctta taaaattgta tcttgcatag aaaaagctga aatatcggat gcgttttaat
    46381 tatcaactat aaatctataa gctttttatt aacaaca
//
LOCUS       DADLEE010000023        45785 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000023 DADLEE010000000
VERSION     DADLEE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.023"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            95..2248
                     /locus_tag="JQH51_001353"
     CDS             95..2248
                     /locus_tag="JQH51_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075750.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="JQH51_001354"
     CDS             2301..4031
                     /locus_tag="JQH51_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075751.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQH51_001355"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQH51_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY6075752.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="JQH51_001356"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="JQH51_001356"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY6075753.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="JQH51_001357"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="JQH51_001357"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY6075754.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQH51_001358"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQH51_001358"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY6075755.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="JQH51_001359"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="JQH51_001359"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY6075756.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="JQH51_001360"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="JQH51_001360"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY6075757.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="JQH51_001361"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="JQH51_001361"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY6075758.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="JQH51_001362"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="JQH51_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY6075759.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQH51_001363"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQH51_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY6075760.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQH51_001364"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQH51_001364"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY6075761.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQH51_001365"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQH51_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY6075762.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQH51_001366"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQH51_001366"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY6075763.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQH51_001367"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQH51_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY6075764.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="JQH51_001368"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="JQH51_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY6075765.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="JQH51_001369"
     CDS             16289..16828
                     /locus_tag="JQH51_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY6075766.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /locus_tag="JQH51_001370"
     CDS             17126..18010
                     /locus_tag="JQH51_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY6075767.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="JQH51_001371"
     CDS             complement(18187..18534)
                     /locus_tag="JQH51_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY6075768.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="JQH51_001372"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="JQH51_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY6075769.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="JQH51_001373"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="JQH51_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY6075770.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="JQH51_001374"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="JQH51_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY6075771.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="JQH51_001375"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="JQH51_001375"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY6075772.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="JQH51_001376"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="JQH51_001376"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY6075773.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="JQH51_001377"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="JQH51_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="JQH51_001378"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="JQH51_001378"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY6075774.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="JQH51_001379"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="JQH51_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY6075775.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="JQH51_001380"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="JQH51_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY6075776.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="JQH51_001381"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="JQH51_001381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY6075777.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="JQH51_001382"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="JQH51_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY6075778.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="JQH51_001383"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="JQH51_001383"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY6075779.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="JQH51_001384"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="JQH51_001384"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY6075780.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="JQH51_001385"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="JQH51_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY6075781.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="JQH51_001386"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="JQH51_001386"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY6075782.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="JQH51_001387"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="JQH51_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY6075783.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="JQH51_001388"
     CDS             complement(39992..40447)
                     /locus_tag="JQH51_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075784.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="JQH51_001389"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="JQH51_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="JQH51_001390"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="JQH51_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY6075785.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="JQH51_001391"
     CDS             complement(41290..41967)
                     /locus_tag="JQH51_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY6075786.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="JQH51_001392"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="JQH51_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY6075787.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="JQH51_001393"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="JQH51_001393"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY6075788.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="JQH51_001394"
     CDS             complement(43173..43631)
                     /locus_tag="JQH51_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY6075789.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="JQH51_001395"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="JQH51_001395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY6075790.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="JQH51_001396"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="JQH51_001396"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY6075791.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11901 a        11944 c        11202 g        10738 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgaga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DADLEE010000024        45630 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000024 DADLEE010000000
VERSION     DADLEE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45630
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.024"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JQH51_001397"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JQH51_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY6075792.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JQH51_001398"
     CDS             complement(1151..1237)
                     /locus_tag="JQH51_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY6075793.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JQH51_001399"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JQH51_001399"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY6075794.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JQH51_001400"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JQH51_001400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY6075795.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JQH51_001401"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JQH51_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY6075796.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="JQH51_001402"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="JQH51_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY6075797.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="JQH51_001403"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="JQH51_001403"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY6075798.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="JQH51_001404"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="JQH51_001404"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY6075799.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="JQH51_001405"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="JQH51_001405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY6075800.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="JQH51_001406"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="JQH51_001406"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY6075801.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JQH51_001407"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JQH51_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY6075802.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JQH51_001408"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JQH51_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY6075803.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /locus_tag="JQH51_001409"
     CDS             11797..13452
                     /locus_tag="JQH51_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY6075804.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWLEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="JQH51_001410"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="JQH51_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY6075805.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="JQH51_001411"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="JQH51_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY6075806.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="JQH51_001412"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="JQH51_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY6075807.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="JQH51_001413"
     CDS             complement(16981..17745)
                     /locus_tag="JQH51_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6075808.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="JQH51_001414"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="JQH51_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY6075809.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="JQH51_001415"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="JQH51_001415"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY6075810.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="JQH51_001416"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="JQH51_001416"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY6075811.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="JQH51_001417"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="JQH51_001417"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY6075812.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="JQH51_001418"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="JQH51_001418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY6075813.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="JQH51_001419"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="JQH51_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY6075814.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="JQH51_001420"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="JQH51_001420"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY6075815.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JQH51_001421"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JQH51_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY6075816.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="JQH51_001422"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="JQH51_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY6075817.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="JQH51_001423"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="JQH51_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY6075818.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="JQH51_001424"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="JQH51_001424"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY6075819.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="JQH51_001425"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="JQH51_001425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY6075820.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="JQH51_001426"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="JQH51_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY6075821.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="JQH51_001427"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="JQH51_001427"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY6075822.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JQH51_001428"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JQH51_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY6075823.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JQH51_001429"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JQH51_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY6075824.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JQH51_001430"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JQH51_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY6075825.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="JQH51_001431"
     CDS             complement(40421..41815)
                     /locus_tag="JQH51_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6075826.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JQH51_001432"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JQH51_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY6075827.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="JQH51_001433"
     CDS             complement(42158..43444)
                     /locus_tag="JQH51_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6075828.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="JQH51_001434"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="JQH51_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="JQH51_001435"
     CDS             complement(44451..45221)
                     /locus_tag="JQH51_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY6075829.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        11172 a        12494 c        11753 g        10211 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcttga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgtcc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca
//
LOCUS       DADLEE010000025        45345 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000025 DADLEE010000000
VERSION     DADLEE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45345
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.025"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            97..396
                     /locus_tag="JQH51_001436"
     CDS             97..396
                     /locus_tag="JQH51_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6075830.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="JQH51_001437"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="JQH51_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY6075831.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="JQH51_001438"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="JQH51_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY6075832.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="JQH51_001439"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="JQH51_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY6075833.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="JQH51_001440"
     CDS             complement(2535..2879)
                     /locus_tag="JQH51_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY6075834.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="JQH51_001441"
     CDS             3031..3261
                     /locus_tag="JQH51_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075835.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="JQH51_001442"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="JQH51_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY6075836.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="JQH51_001443"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="JQH51_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY6075837.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="JQH51_001444"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="JQH51_001444"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY6075838.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="JQH51_001445"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="JQH51_001445"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY6075839.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="JQH51_001446"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="JQH51_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY6075840.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="JQH51_001447"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="JQH51_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY6075841.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="JQH51_001448"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="JQH51_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY6075842.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="JQH51_001449"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="JQH51_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /locus_tag="JQH51_001450"
     CDS             12505..13242
                     /locus_tag="JQH51_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY6075843.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="JQH51_001451"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="JQH51_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY6075844.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(13586..13687)
                     /locus_tag="JQH51_001452"
     CDS             complement(13586..13687)
                     /locus_tag="JQH51_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075845.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="JQH51_001453"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="JQH51_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY6075846.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="JQH51_001454"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="JQH51_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="JQH51_001455"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="JQH51_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY6075847.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="JQH51_001456"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="JQH51_001456"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY6075848.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="JQH51_001457"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="JQH51_001457"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY6075849.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="JQH51_001458"
     CDS             complement(19400..19828)
                     /locus_tag="JQH51_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6075850.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="JQH51_001459"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="JQH51_001459"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY6075851.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20539..20615)
                     /locus_tag="JQH51_001460"
     tRNA            complement(20539..20615)
                     /locus_tag="JQH51_001460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20579..20581),aa:Arg,seq:acg)
     gene            complement(20680..20756)
                     /locus_tag="JQH51_001461"
     tRNA            complement(20680..20756)
                     /locus_tag="JQH51_001461"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20720..20722),aa:Arg,seq:acg)
     gene            complement(20760..20852)
                     /locus_tag="JQH51_001462"
     tRNA            complement(20760..20852)
                     /locus_tag="JQH51_001462"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20816..20818),aa:Ser,seq:gct)
     gene            complement(21168..21353)
                     /gene="csrA"
                     /locus_tag="JQH51_001463"
     CDS             complement(21168..21353)
                     /gene="csrA"
                     /locus_tag="JQH51_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY6075852.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21588..24218)
                     /gene="alaS"
                     /locus_tag="JQH51_001464"
     CDS             complement(21588..24218)
                     /gene="alaS"
                     /locus_tag="JQH51_001464"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY6075853.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24346..24846)
                     /gene="recX"
                     /locus_tag="JQH51_001465"
     CDS             complement(24346..24846)
                     /gene="recX"
                     /locus_tag="JQH51_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY6075854.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25089..26150)
                     /gene="recA"
                     /locus_tag="JQH51_001466"
     CDS             complement(25089..26150)
                     /gene="recA"
                     /locus_tag="JQH51_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY6075855.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26230..26727)
                     /gene="pncC"
                     /locus_tag="JQH51_001467"
     CDS             complement(26230..26727)
                     /gene="pncC"
                     /locus_tag="JQH51_001467"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY6075856.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26872..27957)
                     /gene="mltB"
                     /locus_tag="JQH51_001468"
     CDS             complement(26872..27957)
                     /gene="mltB"
                     /locus_tag="JQH51_001468"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY6075857.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28213..28775
                     /gene="srlA"
                     /locus_tag="JQH51_001469"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28213..28775
                     /gene="srlA"
                     /locus_tag="JQH51_001469"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            28772..29731
                     /gene="srlE"
                     /locus_tag="JQH51_001470"
                     /gene_synonym="gutE"
     CDS             28772..29731
                     /gene="srlE"
                     /locus_tag="JQH51_001470"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY6075858.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            29742..30113
                     /gene="srlB"
                     /locus_tag="JQH51_001471"
                     /gene_synonym="gutB"
     CDS             29742..30113
                     /gene="srlB"
                     /locus_tag="JQH51_001471"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY6075859.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30117..30896
                     /gene="srlD"
                     /locus_tag="JQH51_001472"
     CDS             30117..30896
                     /gene="srlD"
                     /locus_tag="JQH51_001472"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY6075860.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31001..31360
                     /gene="gutM"
                     /locus_tag="JQH51_001473"
     CDS             31001..31360
                     /gene="gutM"
                     /locus_tag="JQH51_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY6075861.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31427..32200
                     /gene="srlR"
                     /locus_tag="JQH51_001474"
                     /gene_synonym="gutR"
     CDS             31427..32200
                     /gene="srlR"
                     /locus_tag="JQH51_001474"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY6075862.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32193..33158
                     /gene="gutQ"
                     /locus_tag="JQH51_001475"
     CDS             32193..33158
                     /gene="gutQ"
                     /locus_tag="JQH51_001475"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY6075863.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33155..34669)
                     /gene="norR"
                     /locus_tag="JQH51_001476"
     CDS             complement(33155..34669)
                     /gene="norR"
                     /locus_tag="JQH51_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY6075864.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            34856..36295
                     /gene="norV"
                     /locus_tag="JQH51_001477"
     CDS             34856..36295
                     /gene="norV"
                     /locus_tag="JQH51_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY6075865.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36292..37425
                     /gene="norW"
                     /locus_tag="JQH51_001478"
     CDS             36292..37425
                     /gene="norW"
                     /locus_tag="JQH51_001478"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY6075866.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37553..39805)
                     /gene="hypF"
                     /locus_tag="JQH51_001479"
     CDS             complement(37553..39805)
                     /gene="hypF"
                     /locus_tag="JQH51_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY6075867.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(39958..40485)
                     /gene="hydN"
                     /locus_tag="JQH51_001480"
     CDS             complement(39958..40485)
                     /gene="hydN"
                     /locus_tag="JQH51_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY6075868.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40634..41647)
                     /locus_tag="JQH51_001481"
     CDS             complement(40634..41647)
                     /locus_tag="JQH51_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6075869.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            complement(41658..41777)
                     /locus_tag="JQH51_001482"
     CDS             complement(41658..41777)
                     /locus_tag="JQH51_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAY6075870.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(41787..43496)
                     /locus_tag="JQH51_001483"
     CDS             complement(41787..43496)
                     /locus_tag="JQH51_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY6075871.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43514..44437)
                     /gene="hycD"
                     /locus_tag="JQH51_001484"
     CDS             complement(43514..44437)
                     /gene="hycD"
                     /locus_tag="JQH51_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY6075872.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44440..>45345)
                     /locus_tag="JQH51_001485"
     CDS             complement(44440..>45345)
                     /locus_tag="JQH51_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075873.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT        10810 a        12228 c        11490 g        10817 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    20581 tagccgagta ctctatccag ctgagctacg gatacatcgg gaaacttact ttactgcaga
    20641 ttttttgata ccgctactaa agccgtatca agtaagagat ggtgcatccg ggaggattcg
    20701 aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcaaat
    20761 ggcggtgagg cggggattcg aaccccggat gcagcttttg accgcatact cccttagcag
    20821 gggagcgcct tcagcctctc ggccacctca ccacacgcct cttacgagtg cttcgaagaa
    20881 cttgtttatc gctcatcatc gctgcgtggc gcacatatta ctttctgaga cttataagtc
    20941 aaacaatttt tcccacactt ttatcgtttg cacacttcac gctcaattag tctgtaaaag
    21001 cgtcaaaaag agtgttttat ctacagaaaa acggaggtct ggcagatagc agtaatgcaa
    21061 aaaaatggag acttaagttg aatgaacggg agtaaagcga aaagactata gagtgaagga
    21121 gaaattttga gggtgcgtct caccgataaa gatgagacgc ggaaagatta gtaactggac
    21181 tgctgggatt tttcagcctg gatacgctgg tagatctctt cacggtgaac agaaacttcc
    21241 ttcggggcat ttacgccaat acgtacctgg ttgcccttta cccctaaaac tgtcacggtg
    21301 acctcatccc caatcatgag ggtctcacca actcgacgag tcagaatcag cattctttgc
    21361 tccttgaaag attaaaagag tcgggtctct ctgtatcccg gcattatcca tcatataacg
    21421 ccaaaaagta agcaatgaca aacacattac atgtaagcag tcatggcatt acattctgtt
    21481 aaacctaagt ttagccgata tacacaactt caacctgact ttatcgttgt cgatagcgtt
    21541 aatgcgaatg ccgtgaagcg agtccacggc attgcctgac gcttatatta ttgcaatttc
    21601 gcgctgaccc agcctttcac actggctaac gctgcaggta aggccgcagc atccgtacca
    21661 ccggcttgcg ccatgtcagg acgtccacca cccttgccgc ccacctgctg agcgaccata
    21721 ccaatcagtt cccctgcttt cacacgatct gtgacgtcct tagatacgcc tgcaatcaga
    21781 gaaaccttac cttcggctac cgttgccagc acgataattg tcgaccccag ctgatttttt
    21841 aaatcgtcaa ccatggtacg caacattttc ggctcaacac cgctaagctc gctaaccaac
    21901 agcttaacac cattaacatc aattgcctta ctggaaagat ttgcgctctc ctgtgcggca
    21961 gcttgttctt taagctgttg tagttctttt tctaactgac gcgtacgttc cagtactgag
    22021 cgcactttat ccgccagatt attgctatcg cctttcagca gatgcgcgac ttcgcttaag
    22081 cgatcgctgt ctgcatgaac ggtggtgata gcgccttctc cggttaccgc ttcgatacga
    22141 cgaacgcctg cagcagtacc cgattcagag atgatgcgga acagaccaat atcaccagtg
    22201 cggctggcgt gagtaccgcc acacagctcg gtggagaaat cgcccatgct cagcacgcgt
    22261 acacgctcat catacttctc gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg
    22321 agattcatga tgttggtttc gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct
    22381 tcgaccgcac gaatctcttc cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc
    22441 accttgtcgt taaccagtga acctttctgc gatacatgag tccccagaac ctggcgcagc
    22501 gcagcgtgca tcaggtgcgt tgcggagtga ttcagacgaa tacgggcgcg acgagcctca
    22561 tcaatatcag cctgcacagc gtcgcccact ttcagagaac ccgcagcaag tttaccgatg
    22621 tgaccaatcg cctggccgta tttctgcgta tcttccaccg cgaaggagaa gttagcgcct
    22681 ttcagttcgc ctttatcacc aacctgaccg ccggattccg catagaatgg cgtttgatcc
    22741 agcacgacca cagcttcctg gccggcattg atggcatcaa ccgctttacc atcaacaaac
    22801 agcgcggtca ctttgccgtt cagttccaga tggtcatagc ctttaaattc agatgcacta
    22861 tcaacacgga tcattgcgtt gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga
    22921 cgctgctctt ccattgctgc ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga
    22981 caaacatcag ccgtcaggtc aaccgggaag ccataggtgt cgtacagacg gaaagccgtt
    23041 tcaccatcca gcgtatcacc agaaagtttt gccagctctt catccagcaa cgccagaccg
    23101 cgctccagag tacgggcaaa ctgctcttct tcagtcttca gcacctgctc aacctgcgcc
    23161 tgctggcgtt tcaggtcttc acccgcagag cccataacgt cgatcagcgg accaaccagt
    23221 ttgtagaaga aggtttcttt cgcgccgagc atattgccgt gacgcactgc gcgacgaatg
    23281 atacgacgca gtacataacc acggttttca ttggacggca ttacgccatc cgcgatcagg
    23341 aacgcacaag aacgaatgtg gtcagcgatt acgcgcagcg atttattgct cagatcggtc
    23401 gcgccagtga ctttcgctac cgcctggatc aacgtgcgga acaggtcgat gtcatagtta
    23461 gagttaacgt gttgcagcac cgcagcaata cgctccagac ccataccggt atctacagac
    23521 ggcttcggca gcggttccat cgtgccatct gcctggcggt tgaactgcat gaagacgatg
    23581 ttccagatct caatgtagcg gtcgccgtct tcttccggac ttcccggagg gccaccccaa
    23641 atgtggtcgc cgtgatcgta gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc
    23701 atctgccaga agttgtcaga tgcgtatggc gcacctttgt tatcgccgat gcgaataata
    23761 cgttcgcgcg ggatcccgac ttctttttcc cagatttcgt aggcttcgtc gtcgctttca
    23821 tagacggtaa cccacagacg ctctttcggc agggcaaacc atttttcgct ggtcagcagt
    23881 tcccatgcaa actgaatggc atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc
    23941 atttcgaaga aggtatggtg acgcgcggtg taaccgacgt tttccaggtc gttgtgttta
    24001 ccacccgcac gcacgcagcg ttgggaagtg gtagcgcggg aataattacg cttgtcgagc
    24061 ccaaggaaca catccttgaa ctggttcatc ccggcgttgg taaacaacaa agttgggtcg
    24121 ttatggggta ccagggagct gctggcaact acctgatgtc ccttactatg gaaaaagtcg
    24181 agaaacgcct gacggatctc agcggtgctc ttgctcataa ttatcctgaa atcaagctaa
    24241 cgaaatatcg ccaccagctc cagcgtgtct taaccgccgg gctggtaact gaaaagtggg
    24301 aataagataa gttttcttga ctgggaagta aaataccgta tgcgttcagt cggcaaaatt
    24361 tcgccaaata tcctggatat cttccatcag atagccacga tagagcagaa aacgctggat
    24421 cttaactttt tctgaaaaga cagttggcaa aggttcacca tattttcgcg tcgcctgatc
    24481 gcgcgccagt gcgcaccagt cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg
    24541 ggaaatacct ttctgattca gttcctgacg aatacgtgca ggtccatacc ctttgcggct
    24601 acggctggcg ataaagcgcg caacaaatcg gctgtcatcg agatagccat gttcatggca
    24661 ccaggcaata acgcgctcgt aatcttctgc cgtagcatca atctcttctg ggccattttt
    24721 gcccataatc ggtgccgcga gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc
    24781 cagaatgcgt accgcacgat ccaacaggcg agcatatgcc gggcgacggg atgttgattc
    24841 tgtcatggca tatccttaca acttaaaaaa gcaaaagggc cgcagatgcg ccccaggttt
    24901 tgctgataaa cagatttaat agtcaatgag tatgcagaat gttcccgcag gcccaattcg
    24961 ttagaggatc gaggtgagag taatttttca cgccagtgct ggcctgttcc cctcacccta
    25021 accctctccc caaaggggcg aggggaccgt ccgtgcacac attatctatt atcatcaata
    25081 acgaacgatt aaaaatcttc gttagtttct gctactcctt cgctgtcatc tacagagaaa
    25141 tccggcgttg agttcgggtt gctcagcagc aactcacgta ctttcttctc gatctctttc
    25201 gcggtttccg ggttatcttt cagccaggca gttgcattcg ctttaccctg accgatcttc
    25261 tcacctttgt agctgtacca cgcgcctgct ttctcgatca gcttctcttt tacgcccagg
    25321 tcaaccagtt cgccgtagaa gttgatacct tcgccgtaga ggatctgaaa ttcagcctgt
    25381 ttaaacggtg cagcgatttt gttcttcacc actttcacgc gggtttcgct acccaccacg
    25441 ttttcgccct ctttcaccgc gccgatacga cggatgtcga gacgaacaga ggcgtagaat
    25501 ttcagcgcgt taccaccggt agtggtttcc gggttaccga acatcacacc aattttcata
    25561 cggatctggt tgatgaagat cagcagcgtg ttggactgct tcaggttacc cgccagctta
    25621 cgcatcgcct ggctcatcat acgtgccgca aggcccatgt gagagtcgcc gatttcgcct
    25681 tcgatttccg ctttcggcgt cagtgccgcc acggagtcaa cgacgataac gtctactgcg
    25741 ccagaacgcg ccagggcgtc acagatttcc agtgcctgct cgccggtatc cggctgggag
    25801 cacagcaggt tatcgatatc gacgcccagt ttacgtgcgt agattgggtc cagcgcgtgt
    25861 tcagcatcga taaacgcaca ggttttacct tcacgctgcg ctgcggcgat cacctgcagc
    25921 gtcagcgtgg ttttaccgga agattccggt ccgtagattt cgacgatacg gcccatcggc
    25981 agaccacctg ccccaagcgc gatatccagt gaaagcgaac cggtagagat ggtttccaca
    26041 tccatggaac ggtcttcacc caggcgcatg atggagcctt taccaaattg tttctcaatc
    26101 tggcccagtg ctgccgccaa cgctttctgt ttgttttcgt cgatagccat ttttactcct
    26161 gtcatgccgg gtaataccgg atagtcaata tgttctgttg aaacaattat actgtatgct
    26221 catacagtat caagtgtttt gtagaaattg ttgccacaag gtctgcaatg catacgcagt
    26281 agcctgacga cgcaccgcat cacggtcgcc gctgaagcat tcccgccggg taatgccttc
    26341 accgcgggca gtggcaaaag caaaccagac ggtgccgaca ggcttctctt cactgccgcc
    26401 atccggcccg gcgataccac taatagacac ggcataatca gcacgagccg ctttcagtgc
    26461 acctatcgcc atttccacca cgacgggttc actcaccgcg ccatgctgcg ccagcgtctc
    26521 ttcgcgtacg ccgatcatct gcgctttggc ttcgttactg taggtgacaa atccgcgttc
    26581 aaaccaggcg gagctaccgg caatatcggt aatcactttc gctacccaac caccggtaca
    26641 agactcggca gttgttacgg ttgcgccacg ggctttcagc gcctgcccaa cctgttcgct
    26701 taactgcatc agttcactgt cagtcatcac aacctctgtc agtcaaaaat ttatgccgga
    26761 caagatagca ctttcgtcag gaagatggtg ttggcgttgc aattttacga ggaggattca
    26821 gaaaaaagct gattagccag agggaagctc acgcccccct cttgtaaata gctactgtac
    26881 tcgcgccagc gccacggctt gtcctaactg ccagaccgcc attgcgtaat gggtgctgtg
    26941 gttgtaacgg gtgatggtgt agaagttcgg cagaccgtac cagtactggt agccggtgcc
    27001 aacatccaga cgcagcaggc tggcttgttg atggttgccc agcggctgct gtggcgttaa
    27061 acctgcggcg gcaagctgcg aaatgctgta cttagttttg aaaccatttg gcaagcctgg
    27121 agcctgaccg tttgccatta tcgcgacctg atcgcctttc acccagccgt gcgctttgaa
    27181 atagttcgcc acgctaccga tcgcatcaac cggatcccac aggttgatat gcccgtcgcc
    27241 gctgaaatct accgcatatt gtttgtaaga cgacggcata aactgtccgt agcccatcgc
    27301 cccggcaaag gaacctttca gattgagcgg atcgtcctgc tcgtcgcgcg ccatcagcag
    27361 gaaggtttcc agctcgccag agaaatactc cgcgcggcgt gggtagttaa atgacagcgt
    27421 tgccagcgca tcgaggatgc gagttttccc catcacgcgc ccccagcggg tttcaacgcc
    27481 gataatcccg acgataattt ccggcggtac gccatacacc tgccacgcgc gattcaacgc
    27541 atcttcatac tgattccaga aaaccacacc gttctgcacg ttgtccggcg taataaattt
    27601 tttgcgataa cgcagccatg cgccgttcgg acctgatggc ggtttcaccg atgtggttgg
    27661 tgcctggtta tccatcagcc gcagtaccga atccagacgc ttcgcctggg agagaatttc
    27721 ctgcaactgc tgacgatcga aaccgtgttt gttcaccatt ttgtcgatga actgctgggc
    27781 attcgggtta ttagcgaaat cgccgcccat ctgcatcaca ttgtgctgcg gctcaagcag
    27841 gaagccgcca gacggcgttc cggtggtcgt ttcagtctca gtaggttttg gcttgctgct
    27901 acaggcggca agcaacacaa aaaggggaag caatgttaca taacgacgct tgaacatgag
    27961 gggtccattt aacagattca accaggggca agtatggtaa agcatcacac cccgcacaag
    28021 gaagcggtag tcactgcccg atacggactt tacataactc aactcattcc cctcgctata
    28081 cttttattca aactttcaaa ttaaaatatt tatctttcat tttacgatca aaataacact
    28141 tttaaacctt tcaacctgat tagattaggt tgccgtttgg taataaaaca ataaatcctg
    28201 aaggagagaa caatgataga aaccattact catggtgcag agtggtttat cgggctgttc
    28261 caaaagggcg gagaggtgtt taccgggatg gtgaccggca ttcttccgct gttgattagc
    28321 ctgcaggtta tcatgaacgc actgattaat tttatcggtc agcatcgtat tgaacgtttt
    28381 gctcaacgtt gcgccggtaa ccctgtttcc cgttacctac tgttaccgtg cattggcacg
    28441 tttgtctttt gcaatccgat gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa
    28501 cccagctact acgcggcggc ctcttatagc tgccactcaa tgaatggcct cttcccccat
    28561 atcaaccctg cgaactgttt gtttatcttg gcattgccag cggtctgaca acgctgaacc
    28621 tgccacttgg cccactggcg gtgagttatc tgctggttgg tctggtcacc aatttcttcc
    28681 gcggctgggt gaccgatctg accaccgcca tttttgagaa aaagatgggc attcaacttg
    28741 aacaaaaagt tcacctggca ggagcaacat catgacgcgt attcggatcg aaaaaggaac
    28801 gggtggctgg ggcggcccgc ttgagctgga agccacgccg ggcaaaaaaa tcgtctatat
    28861 caccgccggt acccggcctg cgattgttga caaactggca caacttactg gctggcaggc
    28921 tattgacgga tttaaagaag gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga
    28981 ctgtggcggc acattacgct gcggcatcta tccgaaacga cgtattccca ccattaatat
    29041 ccactcgacg ggcaagtccg gtccgctggc gcagtacatt gtggaagata tttatgtctc
    29101 tggcgtaaaa gaagaaaaca tcactgtagt gggtgatgcg acaccacaac cctcttccgt
    29161 gggccgtgac tatgacacca gtaagaaaat caccgaacaa agcgatggtt tactggcgaa
    29221 ggtgggaatg ggcatggggt ccgccgttgc cgtgttgttt cagtctggtc gtgacaccat
    29281 cgacactgta ttaaaaacca ttctgccgtt tatggcattc gtctcggcgc tcattggcat
    29341 cattatggct tctggccttg gtgactggat tgcccacggt cttgctccgc tagcgagcca
    29401 tccactgggt ctggtcatgc tggcgctcat ctgctccttc ccactgcttt cacctttcct
    29461 cggcccaggc gcagttatcg cacaggttat cggcgtattg attggcgtgc agattggtct
    29521 cggcaatatt ccgccgcatc tggctttacc ggcactgttt gccatcaacg cgcaggcggc
    29581 ttgcgacttc atcccggtcg gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt
    29641 cggtgtccct tctgtactgg tgagccgctt tttaaccggc gcgccgactg tactgatcgc
    29701 ctggtttgtc tccggtttta tctatcaata gaggctgaaa catgaccgtt atttatcaga
    29761 ccaccatcac ccgtatcggc gcgagtgcca ctgacgccct cagcgaccag atgctcatca
    29821 cctttcgtga aggcgcgcca gcggacctcg aagagtattg cttcattcat tgccacggcg
    29881 agttgaaagg tgcactccat cccggtttgc aattttcact cgggcagcat cgctatccgg
    29941 tgaccgctgt tggcagcgtg gcggaagaca accttcgcga actgggtcat gtcaccctgc
    30001 gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt ccatgtggca ggccctgtac
    30061 ccgacgatat cgcgccggga tcggttttga agtttgaatc tgttaaggag taaaaaatga
    30121 atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc
    30181 tggctgccga ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa gccgcaaatg
    30241 tggcacaaga aattaacgcc gaatatggtg aaggtatggc gtacggtttt ggtgctgacg
    30301 ccactagcga gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc tttggtcgcg
    30361 tggatttgct ggtctatagc gccggaatag caaaagcagc ctttatcagc gacttccagc
    30421 tcggcgattt tgaccgttcg ctacaagtga atctggtggg ttatttcctg tgtgcccgtg
    30481 aattttcgcg tttgatgatc cgcgacggga ttcagggtcg cattattcag atcaactcga
    30541 aatccggcaa agtgggcagc aaacacaact ctggctacag cgcagcgaaa tttggtggcg
    30601 tcgggctgac tcaatcactg gcgctggatc tggcggagta cggcattacg gtgcattcac
    30661 tgatgctcgg taacctgctg aaatcgcgga tgttccagtc actgttgcca caatacgcga
    30721 ccaagctggg tatcaaaccg gatcaagtcg agcagtatta catcgacaaa gtaccgctca
    30781 aacgcggctg cgattatcaa gatgtgctga atatgctgct gttctacgcc agtcctaagg
    30841 cgtcgtactg caccggacag tcgatcaatg tcaccggcgg tcaggtgatg ttctgatcaa
    30901 cagcggagat ccgttaagga tctccgtgag actatagaat gcctgatgcg ctacgctcat
    30961 caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac
    31021 cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt
    31081 taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtggacc gttccagcgg
    31141 gcgctttaaa ccgcgggtcg tggtcgccat cgcgctggac gatcagcagt gcatcgtcga
    31201 caccttgttt atgaaaggac tgactgtctt cgcccgaccg caaaaaattc ccgcaattac
    31261 cggcatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca
    31321 gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt
    31381 gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca
    31441 gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt
    31501 ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca ttctggaaca
    31561 tgccggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc
    31621 gcctatcgat cataaaacgc tcatcaacac ccacaagaaa gagctgattg cagaagctgc
    31681 cgttagtttt atccatgatg gcgattcgat cattcttgat gctggcagta ccgttttgca
    31741 gattgttccc ctgctctcgc gctttaataa catcacggtg atgaccaaca gcctgcatat
    31801 cgtcaatgcg ctatccgaac tggataacga acaaactatc ctgatgccag gcggaacatt
    31861 tcgcaaaaaa tcggcctcat ttcacgggca actggcagag aatgccttcg agcatttcac
    31921 cttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg taaccacgtt
    31981 caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat
    32041 ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt
    32101 cgataagcta attaccgacg caggtatcga tccggcgttt cgtcaggcgc tggaagagaa
    32161 agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg aacacgggac
    32221 gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt ctgggcgatg
    32281 attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg gtttcgggaa
    32341 ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac acttgccagt accggcacac
    32401 cggctttttt tgtccatccg gcagaagcac tgcacggcga tctggggatg attgaaagcc
    32461 gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat ctgattattc
    32521 cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc
    32581 tgggcctggc ggcgaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc
    32641 accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg
    32701 cggtcatgca ggcgcgcggt tttaatgaag aagattttgc ccgctcccac ccagctgggg
    32761 cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgtcgtgat gacgctatcc
    32821 cacaggtagc gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc
    32881 tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg
    32941 atttacgtcg ctggctggta ggcggcggcg cactcaccac gccagttaat gaagcgatga
    33001 cgacaggcgg caccacatta caggcacaaa gtcgcgccat cgacgccaaa gaagttctga
    33061 tgaaacgcaa aatcactgcc gcaccagtgg tagatgaaaa cggcaaactc accggcgcaa
    33121 ttaacctgca ggatttctat caggccggga ttatttaatc cttcaatccc agacgtttcg
    33181 ccagccgatg caggttggcg acgtcagttt ccagcattcg cgcacaggct gcccagttgt
    33241 gatgattttg tgccagtgcc tggcgaatag tttcacgctg gaacgcttct gtcgcttcac
    33301 gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg cggcggcaac gtcacctcag
    33361 gaaaagcaaa atgttgcgcc tcaagaatca cttcatcgcc gttgcgggtg gctctcgcca
    33421 gcactaccgc ccgatgaata gcatgttcca gttcgcgcac gtttcccgga aaactgtagt
    33481 gttgcagtaa atttcgcgcc ccggcactta ataccacgcg ggagagcccc tgccgcaaac
    33541 gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg
    33601 gcggcaccga aagtggaaac acgctcaggc gatgaaacaa atcggcgcgg aatcgccctg
    33661 ccagcacctc ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca tcgacccgca
    33721 aactacggtc atcgccaacg cgctgaatat cgccatactg caacaccctc agcagcttgg
    33781 cctgcaatgc caacgacaac tcgccgatct catcgagaaa cagagtaccg ttatccgcca
    33841 tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc acatgcccga
    33901 acaactcact ttccgccaca ctttccggca gtgcggcaca gttgagatag accagcggat
    33961 tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta ccggttcccg
    34021 tctcaccgct gatcagaacg ttgagatcgg acgccgccac aatctcaatc tcttttttca
    34081 gttgcgtcat gccaggggac aagccaatca tctgcgtctg tttcaccgct tcaaacggcg
    34141 tggcatcgcc tggcagcata ttctggcttt ccagttgttc aatcagcaac gcattgctta
    34201 acgctcccgc cgccagcgcg gcaatcagcc gtaactcttc gtcgctgaaa acatcgaact
    34261 gatcgggctg catcccgtcg agcgtcagtg cgccgatcag gttttgtccg acaaacaatg
    34321 gcagaccaac gcaggcgtga accttcagac tctcctgccc aggaatcaaa ccgtcatagg
    34381 gatcgggcaa ttcgctgtct gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt
    34441 ccagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttt gccagaccgt
    34501 cgatggcaag cggaataaac tgccgcgaat cgtaacgtag caacgcagac gcatcgcact
    34561 ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga ccaatcccac
    34621 gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac
    34681 tgtcaatttg actatagata ttgtcatatc gaccatttga ttgatagtca ttttgactac
    34741 tcattaatga gcataatttt atttatagag taaaaacaat cagataagaa actggcacgc
    34801 aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc
    34861 tattgtggtg aaaaataaca ttcattgggt gggtcaacgt gactgggaag tgcgtgattt
    34921 tcacggcacg gaatataaaa cgctgcgcgg cagcagctac aacagctacc tcatccgcga
    34981 agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa
    35041 cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcagaaga
    35101 ggaccacgcc ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc cgatctactg
    35161 tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt ggaattttaa
    35221 tgtggtgaaa actggcgaca cgctggatat cggcaacggc aaacagctca tttttgtcga
    35281 aacaccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct
    35341 gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt
    35401 ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc tgacgccgtt
    35461 cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac cagtcgatat
    35521 gatagcgacc tcccacggcg tggtctggcg cgataacccg acgcaaattg tcgagctgta
    35581 cctgaagtgg gccgctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc
    35641 gaataacacc cgcatgatgg ctgacgctat cgcccaggga attgcggaaa ccgacccacg
    35701 cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt
    35761 cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa
    35821 aatcgccggg ctggtggagg agatgaccgg attacgcttc cgtaacaaac gcgccagtgc
    35881 tttcggatct cacggctgga gcggcggtgc gatggatcgc ctttccacgc gcctgcagga
    35941 tgcgggtttc gaaatgtcgc tcagcctgaa agccaaatgg cgaccagacc aggacgctct
    36001 ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc
    36061 gcagagcacg gtgaatacgg tagttaaaga agaaacctct gccacgacga cggctgacct
    36121 cggcccacga atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc
    36181 aatgcaggac gttgcgccag gaacgccgtg gagtgaagtc ccggataact tcctctgccc
    36241 ggaatgctcc ctcggcaaag acgtctttga cgaactagca tcggaggcaa aatgagcaac
    36301 ggcattgtga tcatcggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa
    36361 caggacgcca ctattccatt aaccctgatt gccgccgaca gcatagatga gtacaacaaa
    36421 cccgacctca gccatgttat cagtcagggg caacgtgccg acgaccttac ccgccagacg
    36481 gcgggtgaat ttgccgggca gtttaatctg cgcctgtttc cgcacacctg ggtgacggat
    36541 atcgatgccg aagcccatgt ggtgaaaagc cagaataatc agtggcaata cgacaagtta
    36601 gtgctggcaa ccggtgccag cgcctttgtc ccgcctgtgc ctgggcgtga gttaatgctg
    36661 acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc
    36721 gtgttgattg tcggcggtgg tttgattggt agcgaactgg cgatggattt ttgtcgtgca
    36781 ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt aatgccacca
    36841 gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct gctgttgaaa
    36901 tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct ggaccaccag
    36961 cgtagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aacagccctg
    37021 gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc
    37081 agtaatgccg atatttacgc gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg
    37141 ttcctgcagc cgattcaact tagcgggatg gtgctggcaa aaaatcttct cggcaataac
    37201 acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt accgctgcat
    37261 ctggcaggcg aaacccagcg tcaggattta cgctggcaga ttaataccga acgccaggga
    37321 atggtggcgc gcggcgttga cgatgctgac cagcttcgcg cctttgtggt cagtgaggat
    37381 cggatgaaag aggcatttgt attgttgaaa acgttgccgg tgtaggtggg ctactgtgcc
    37441 taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc atgtgtaggc
    37501 cggataaggc atttacgccg catccggcaa gggtgtccaa atgcaacacg ctttatccgt
    37561 tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc agagacaaac
    37621 cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg agataatgcg
    37681 ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc agcgtagtga
    37741 taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc agcgcatcat
    37801 gaaacgccca cgcgcgttga ttaaccagtg cctgccagtt cagccactgc tgccagaaag
    37861 tggcgagatc cagttgattg tccacccgcg gcatcgtcac cggatgcgtc actccgtcgc
    37921 atgaggctgc gagcgcctcc agagcacaag ccgcttcacc ttcataactt aacgtggctg
    37981 gcgcacagcc cagtgccgcc gccactgcat cgaacaaccg cccacacgat gatgccagcg
    38041 gcgcgttaat tccacgctca atggcccgcg ccagtacgct ccagttttgc tgttgcacac
    38101 ttgccgtttc agggtaattc tgccactccg gcacaaagcg caggcactgc gccagcaggt
    38161 ttcgccacgg ctgcttcgcc gccaaatcgc cacccggaag cgccactgca ggcaagccgc
    38221 ccaggtgctc acattcgcga tagttcaccc gcaggcactc gccgccccac aaagcgccat
    38281 tctcccccat accgataccg tcgagcgtca aagcaatgac atcaccgcca tccagcggcc
    38341 actgatgctc tgccagacac gccgctgcat gggcatgatg atgcagtacc gtttgcgtcg
    38401 gcagattcat ttcgctcgcc cactggctgg agacatagcc cggatgcaca tcatgcacaa
    38461 tgtattgcgg agtgaaatcg tagatgtttt gcatcaggcg taacgcttcg cgccactgca
    38521 tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct tgttcaccgc
    38581 gcaccaggca gaaggtattt ttcagatccg cgccaagaca cagcacaggc ggaacatttt
    38641 taaagcccgg aggcaaaacc agcgcatccg gcacataccc ccgcgaacgg cgcagcattt
    38701 cgccgctttc gcgcaccact gaatcatcca tccgctgcac gatgtcgcgg ttatgtagca
    38761 agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg ttcgttgctg atagctggtg
    38821 gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc tgtaacagca
    38881 aatgctggag cgggttcgca ggcaacatca ccccgacttc gttaaggcca ggggcgatat
    38941 catcacaaag ctcaggtacg tattttttat ccaccagcac aatcggcgca gcgggcgtgg
    39001 taagcaactg gcgcgcagcg tctggtaaac tttctgccac tggcaacatg actgccagcg
    39061 gtttcgccgg gcgatgtttg cgctcccgca gtgtcgccac cgcgttactg ttacgtgcat
    39121 cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc atttttaact
    39181 gtgcgatagc tgcctgtaat gccgcctctt gttctgcatg ttcaccatga cttacccatt
    39241 caagatgcgg gccacactcc gggcaggcca ccggctgggc gtggaagcga cgatcgagcg
    39301 ggtcgcggta ctctttgtca caggccggac atagcggaaa cgccgccatc acggtaaacg
    39361 ggcggtcgta aggcatcgcg cgaataatgg tgaaacgcgg gccgcagtgg gtacagttga
    39421 taaacggata acgataacgc cgctcgcctg gggtattcat ttcggcaagg caagcagggc
    39481 aagtggcggc atccgggaca atttgcgtat tcatggtgcc gccggtgctc tggcggatgg
    39541 tgaactcggt gggcagttgt gaccagataa acggctcacg ctcgacgcta tcaatacgcg
    39601 ccagtggcgg acagtgctga tgcaattgaa caagaaacgt ttccgggtct tccagcagcc
    39661 ggacttctac gccatcgccg tcattacata catcgccgtg aagatttaat tgctgtgcca
    39721 gctgccagac aaacggacga aaaccgacgc cctgcacttt gccacgaata cgcagttgga
    39781 caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt gcgtaacccg
    39841 ccctgccgga cacgacagcg tcgtatccgg cagtctcagg tcggcgatac cgcccgctcc
    39901 gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctgt aagattttta
    39961 gaacatcagc gccgtacggc ggcgtttttc tgcgctcagt tgttcaagtt tattacgatc
    40021 gacacaaatc agcgcatggg tcgggcaagc cgccatacac gccgggccgt cttcacgatg
    40081 gttgcacagg tcgcatttat tggcttcggc tttgtcagcc cgtacattca gacccgcgcc
    40141 gctgttgcga atcacaggac gtaccaccac ttccatcgcg ccatacgggc aagccaccac
    40201 gcaggttttg caaccaatgc aacgttcctg catcacagga acaaaccctt tatcacggct
    40261 gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac ggcaaactgt
    40321 cgccgtggaa atgttcacac ctttaatgac atggatacgc ggtaaaaatg tttccggggt
    40381 cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt cacaggtacg
    40441 gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat tctccagcaa
    40501 tgacagttaa tgcgccgata cactcacaaa tcatgccagt ttttaatttg ctgttattta
    40561 aggaaattaa tttctgtaat gcaggaaaaa agatgtcatc gacactagtg acgatgacat
    40621 gtgatgacaa tgtttatcgc gaaagggcaa tgagtgagtc gcggcggatc agttttccgc
    40681 taaaggtttt cggcggtgag aaatccccgc catcgagcat aaaaatcagc cgtccaataa
    40741 tttcctgaat catctcagtc accggaattt ttacgctgga gagcgccgga acgatatagg
    40801 gggcaattgc gatatcatcg aatccgataa ctgacacctg ctctggcacc gctacgccgc
    40861 gctcgtgcaa cgctttgatc gcacctatcg ccatatcgtc gttactggca actaacgcgc
    40921 taaatttagc ccctcgttcg agcaacgttt ctaccccttc ggccccgctg gcgggcgtcc
    40981 atttaccgtt agcgataagt ttttcattga gcgcaatacc atgctgcgac agcgcgtctt
    41041 tatacccggc aagacgttca atgctggtgg gggaatccat cgagccggta aggaaagcaa
    41101 tctcctgatg cccggcgttt atcaactctg ccacggcgtt aaagctggtc tgtttatgat
    41161 cgcaccagac gctatggctg ctattattgc gcaggcggcg attaagcacc attatcggct
    41221 gactgtgcgc gtcaatgatg tcatcaatct catccacgct taaaaaacgc gggtaaatca
    41281 taatggcgtc gcagcgcaaa tccagcagat actgaatcgc ctggcgctct tcttcggcgc
    41341 tgtgtttacc atctgccaat agcaactgtc gccctttctc ttccgccatt cgcgcggcat
    41401 gaaacagtaa ttcactaaaa taaatgccgt gataaagcgt gttggtcact accagcccca
    41461 gcgtctgagt actcttcgcc gacagattgc gggccagcaa atttgggcgg taactgctct
    41521 cttctaccgc ctgaaacacg cgatctttag tctcctggct gacgtagcca ttacctgaaa
    41581 gcacgcggga aacggtcgct tttgaaaccc cggcgcgctt cgccacttcc agcatcgtcg
    41641 tcatcatttt catcccttta cacgcaatca acgcagtgta ctgcaccgtt tgccgattgt
    41701 ccttgcgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt gatgactttt
    41761 ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg ctcaatgctg
    41821 taacgctcga gttctttgta cggcaccact ttgctcttct tcttacgcac atcgactacg
    41881 gtcatgcggt cggtacagga gtagcaaggg tccaggctac cgataatcag cggcgcatag
    41941 gaaacggtat tgccacgcag catgtagcgc agggtcggcc agttcgcgta ggtcgcggca
    42001 cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg gatatcgtcg
    42061 ccgcgcggcg cttcggcaaa gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc
    42121 accatcagcg gaccgcccgg caggttatcc agaccgtagt cgatcatatt cagcgcggta
    42181 tagacctcgt tgattcgcac tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg
    42241 acttccattg gcagcaggcc ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg
    42301 agaccgctgg cgcgaaccat cgggccaacg ttactgaaat cgcgggcgat ttccgggtcc
    42361 agacgaccaa tgccgacggt gcgttgttcc atgttcggcg tactcagcag cacatccacc
    42421 agctcctgca cttcacgacg catttgttgc gccagctggc gggtctggat catgtcatct
    42481 ttcagcagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt acgcgcaccg
    42541 gtaaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg cataaagccg
    42601 gagtcgaagc cggtaaagtg acaggcgagg ccgaggttga gcagatgcgg gtgcaggcgt
    42661 tccacctcca gcagaatggc gcggatcatc tgcgcgcgtt ctggcacctg aatacccatc
    42721 gcgttttcca ccgacgtggt gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca
    42781 cggtcagaga ggaaggtcac ttcgttataa cccatgcggg tttccgccag tttttccata
    42841 ccgcgatgga cgtagaacag acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac
    42901 agacggaagt gacccggttc gtcagaagtg acgtgcagcg gaccaatcgg cacgacgttg
    42961 tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt agtcggtgcc
    43021 ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc cggccagtca
    43081 tccggcagca ccagacgacg ttcatccggc agaccaaccg gaatcaaacc gtacatatcg
    43141 cgcacttcgc gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga cggatactcc
    43201 ggtttgttgg cgtcaacttc gacgcgaacc gttacccaac acttggtgcc cttctccatc
    43261 gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc
    43321 accgacagcc agccaccctg cttgtagtag agaaactcca ccacttccgg cagatagttc
    43381 acctttacgg tgacagtcag ctgatctttg gtctgccagg cgtggtccag cacgacgcct
    43441 agaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc agacatgctc
    43501 aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca aagccaaacc
    43561 cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca
    43621 gcgcgataac caggacgccg accaccagtt tgacgatggc aatcaccagc gccagcagca
    43681 gtccaccgac ggtgaaggtt tccatttgtc cccacggaat aaacaccccg acgaacatct
    43741 gcaacaccac cagctgtttc aggctgatac cccatttcat gacgccaaag ccgctaccgc
    43801 tgtattcgga gagcggacct tcctgcaact cctgctcggc ttccgccagg tcgaacggca
    43861 gtttgcccat ttcgataaaa gtggcgaacg cacaggcgca gagcgccagc accagcggga
    43921 tgctttggct cagcggccag tgataaacgg tgtcggtaat gttgctgatg ttggtggaac
    43981 cggcaacctg tgcggcaacc cacagaccaa gcagcaacat cggttcgacc agcacgccaa
    44041 gcatcgcttc acggctcgca ccgatagcgg taaacgggct accggtatcc agaccggaaa
    44101 tggcaaagaa gaaacgcgcg atggcgaaga gatacagtaa ggtgatcaaa tcgcccagtt
    44161 gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc
    44221 ccaccatcac atacggcgtc aggcggaaca tccagccaga ggcatccggg ccaacgctct
    44281 ggcggcccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc ccgcgacggt
    44341 tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca acggcaaata
    44401 acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc gtgaaacaat
    44461 aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca acgcactacc
    44521 ttcgcactgc cagcccggca ctagcgacac cggattcagc catttgcgca gtttcagcac
    44581 tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca ccattgattt
    44641 ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca ggcggtcgcc
    44701 tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca gcaatgtgat
    44761 cattggttga gaaacggtgg tgttagcagg ctccagaggc agaggtacag cagcagagag
    44821 catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg ccagtgccac
    44881 tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt tggtacgcgg
    44941 cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca gcgcaccggt
    45001 aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg cgccgctatt
    45061 gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt tcagtggcgg
    45121 cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg gcattttctt
    45181 gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga cgctccccgc
    45241 cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca gaccaccgac
    45301 caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgt
//
LOCUS       DADLEE010000026        42863 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000026 DADLEE010000000
VERSION     DADLEE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42863
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.026"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQH51_001486"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQH51_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY6075874.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JQH51_001487"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JQH51_001487"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY6075875.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JQH51_001488"
     CDS             complement(3736..4092)
                     /locus_tag="JQH51_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY6075876.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JQH51_001489"
     CDS             complement(4096..4512)
                     /locus_tag="JQH51_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY6075877.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JQH51_001490"
     CDS             complement(4623..5336)
                     /locus_tag="JQH51_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY6075878.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="JQH51_001491"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="JQH51_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQH51_001492"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQH51_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY6075879.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQH51_001493"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQH51_001493"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY6075880.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /locus_tag="JQH51_001494"
     CDS             complement(9023..10438)
                     /locus_tag="JQH51_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY6075881.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="JQH51_001495"
     CDS             10521..11504
                     /locus_tag="JQH51_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY6075882.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQH51_001496"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQH51_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY6075883.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQH51_001497"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQH51_001497"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY6075884.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14153)
                     /locus_tag="JQH51_001498"
     CDS             complement(13446..14153)
                     /locus_tag="JQH51_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY6075885.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14177..14431)
                     /locus_tag="JQH51_001499"
     CDS             complement(14177..14431)
                     /locus_tag="JQH51_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075886.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="JQH51_001500"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="JQH51_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY6075887.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="JQH51_001501"
     CDS             complement(15306..15515)
                     /locus_tag="JQH51_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY6075888.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..17010)
                     /gene="dinF"
                     /locus_tag="JQH51_001502"
     CDS             complement(15631..17010)
                     /gene="dinF"
                     /locus_tag="JQH51_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY6075889.1"
                     /translation="MPLGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQH51_001503"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQH51_001503"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY6075890.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /locus_tag="JQH51_001504"
     CDS             complement(17747..18115)
                     /locus_tag="JQH51_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY6075891.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="JQH51_001505"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="JQH51_001505"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY6075892.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQH51_001506"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQH51_001506"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY6075893.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQH51_001507"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQH51_001507"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY6075894.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="JQH51_001508"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="JQH51_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQH51_001509"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQH51_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY6075895.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQH51_001510"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQH51_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY6075896.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQH51_001511"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQH51_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY6075897.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="JQH51_001512"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="JQH51_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY6075898.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="JQH51_001513"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="JQH51_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY6075899.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="JQH51_001514"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="JQH51_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY6075900.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="JQH51_001515"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="JQH51_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY6075901.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="JQH51_001516"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="JQH51_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY6075902.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="JQH51_001517"
     CDS             complement(34461..34604)
                     /locus_tag="JQH51_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075903.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="JQH51_001518"
     CDS             34605..34883
                     /locus_tag="JQH51_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075904.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="JQH51_001519"
     CDS             complement(34930..37026)
                     /locus_tag="JQH51_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY6075905.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="JQH51_001520"
     CDS             complement(37026..37763)
                     /locus_tag="JQH51_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY6075906.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="JQH51_001521"
     CDS             complement(37760..38398)
                     /locus_tag="JQH51_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY6075907.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="JQH51_001522"
     CDS             complement(38512..38754)
                     /locus_tag="JQH51_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075908.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="JQH51_001523"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="JQH51_001523"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY6075909.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..42740
                     /gene="lysC"
                     /locus_tag="JQH51_001524"
     CDS             41391..42740
                     /gene="lysC"
                     /locus_tag="JQH51_001524"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY6075910.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10670 a        11026 c        10521 g        10646 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctaggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc aaatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagctaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca
    42661 gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc
    42721 atagtaattt gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga
    42781 ttttcttgtc actatgaggt agcctgagtt taacggacac tccttcctga aatagaatgg
    42841 catcagaagg agctaataat gag
//
LOCUS       DADLEE010000027        40544 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000027 DADLEE010000000
VERSION     DADLEE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.027"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..956)
                     /locus_tag="JQH51_001525"
     CDS             complement(<1..956)
                     /locus_tag="JQH51_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY6075911.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /locus_tag="JQH51_001526"
     CDS             1202..1828
                     /locus_tag="JQH51_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY6075912.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..1980)
                     /locus_tag="JQH51_001527"
     CDS             complement(1825..1980)
                     /locus_tag="JQH51_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY6075913.1"
                     /translation="MSVNGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGEL
                     LKVSPRN"
     gene            complement(2001..2939)
                     /locus_tag="JQH51_001528"
     CDS             complement(2001..2939)
                     /locus_tag="JQH51_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY6075914.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGS"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQH51_001529"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQH51_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY6075915.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="JQH51_001530"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="JQH51_001530"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY6075916.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="JQH51_001531"
     CDS             complement(5018..6346)
                     /locus_tag="JQH51_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6075917.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="JQH51_001532"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="JQH51_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="JQH51_001533"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="JQH51_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY6075918.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="JQH51_001534"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="JQH51_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY6075919.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="JQH51_001535"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="JQH51_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY6075920.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="JQH51_001536"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="JQH51_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY6075921.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="JQH51_001537"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="JQH51_001537"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY6075922.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="JQH51_001538"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="JQH51_001538"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY6075923.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="JQH51_001539"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="JQH51_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY6075924.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="JQH51_001540"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="JQH51_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY6075925.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="JQH51_001541"
     CDS             18267..19166
                     /locus_tag="JQH51_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY6075926.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="JQH51_001542"
     CDS             19172..21604
                     /locus_tag="JQH51_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY6075927.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="JQH51_001543"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="JQH51_001543"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY6075928.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="JQH51_001544"
     CDS             22717..23982
                     /locus_tag="JQH51_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY6075929.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="JQH51_001545"
     CDS             complement(24223..25815)
                     /locus_tag="JQH51_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY6075930.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="JQH51_001546"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="JQH51_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6075931.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="JQH51_001547"
     CDS             complement(27012..28130)
                     /locus_tag="JQH51_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY6075932.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="JQH51_001548"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="JQH51_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HAY6075933.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="JQH51_001549"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="JQH51_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY6075934.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /locus_tag="JQH51_001550"
     CDS             29180..30763
                     /locus_tag="JQH51_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY6075935.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="JQH51_001551"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="JQH51_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY6075936.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="JQH51_001552"
     CDS             complement(31379..31444)
                     /locus_tag="JQH51_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075937.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="JQH51_001553"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="JQH51_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY6075938.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="JQH51_001554"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="JQH51_001554"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY6075939.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="JQH51_001555"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="JQH51_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY6075940.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="JQH51_001556"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="JQH51_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY6075941.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="JQH51_001557"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="JQH51_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY6075942.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36457..38520
                     /gene="ybiO"
                     /locus_tag="JQH51_001558"
     CDS             36457..38520
                     /gene="ybiO"
                     /locus_tag="JQH51_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY6075943.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWGRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHMLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIMLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="JQH51_001559"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="JQH51_001559"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY6075944.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="JQH51_001560"
     CDS             39719..39979
                     /locus_tag="JQH51_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6075945.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="JQH51_001561"
     CDS             40244..>40544
                     /locus_tag="JQH51_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY6075946.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10097 a        10718 c         9866 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DADLEE010000028        40368 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000028 DADLEE010000000
VERSION     DADLEE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40368
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.028"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            231..1664
                     /locus_tag="JQH51_001562"
     CDS             231..1664
                     /locus_tag="JQH51_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY6075947.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JQH51_001563"
     CDS             complement(1672..2730)
                     /locus_tag="JQH51_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6075948.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JQH51_001564"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JQH51_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JQH51_001565"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JQH51_001565"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY6075949.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQH51_001566"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQH51_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY6075950.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQH51_001567"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQH51_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY6075951.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JQH51_001568"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JQH51_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY6075952.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JQH51_001569"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JQH51_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY6075953.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQH51_001570"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQH51_001570"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY6075954.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQH51_001571"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQH51_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY6075955.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQH51_001572"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQH51_001572"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY6075956.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQH51_001573"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQH51_001573"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY6075957.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JQH51_001574"
     CDS             complement(17699..18322)
                     /locus_tag="JQH51_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY6075958.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JQH51_001575"
     CDS             18619..18894
                     /locus_tag="JQH51_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6075959.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JQH51_001576"
     CDS             complement(18971..19213)
                     /locus_tag="JQH51_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY6075960.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JQH51_001577"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JQH51_001577"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY6075961.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JQH51_001578"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JQH51_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY6075962.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQH51_001579"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQH51_001579"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY6075963.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="JQH51_001580"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="JQH51_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /locus_tag="JQH51_001581"
     CDS             24093..24827
                     /locus_tag="JQH51_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6075964.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="JQH51_001582"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="JQH51_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQH51_001583"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQH51_001583"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY6075965.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQH51_001584"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQH51_001584"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY6075966.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQH51_001585"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQH51_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="JQH51_001586"
     CDS             complement(30355..31602)
                     /locus_tag="JQH51_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY6075967.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="JQH51_001587"
     CDS             complement(31624..31950)
                     /locus_tag="JQH51_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY6075968.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQH51_001588"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQH51_001588"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY6075969.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="JQH51_001589"
     CDS             33338..34594
                     /locus_tag="JQH51_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY6075970.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="JQH51_001590"
     CDS             34709..35035
                     /locus_tag="JQH51_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY6075971.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /gene="mntH"
                     /locus_tag="JQH51_001591"
     CDS             complement(35176..36414)
                     /gene="mntH"
                     /locus_tag="JQH51_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY6075972.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="JQH51_001592"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="JQH51_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY6075973.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="JQH51_001593"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="JQH51_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10769 a        10037 c         9323 g        10239 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctt aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgc
//
LOCUS       DADLEE010000029        40030 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000029 DADLEE010000000
VERSION     DADLEE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40030
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.029"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(117..1370)
                     /locus_tag="JQH51_001594"
     CDS             complement(117..1370)
                     /locus_tag="JQH51_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY6075974.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1602..2933
                     /gene="argA"
                     /locus_tag="JQH51_001595"
     CDS             1602..2933
                     /gene="argA"
                     /locus_tag="JQH51_001595"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY6075975.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2995..4821)
                     /gene="recD"
                     /locus_tag="JQH51_001596"
     CDS             complement(2995..4821)
                     /gene="recD"
                     /locus_tag="JQH51_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY6075976.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4821..8363)
                     /gene="recB"
                     /locus_tag="JQH51_001597"
     CDS             complement(4821..8363)
                     /gene="recB"
                     /locus_tag="JQH51_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY6075977.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8356..11244)
                     /gene="ptrA"
                     /locus_tag="JQH51_001598"
     CDS             complement(8356..11244)
                     /gene="ptrA"
                     /locus_tag="JQH51_001598"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY6075978.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11420..14788)
                     /gene="recC"
                     /locus_tag="JQH51_001599"
     CDS             complement(11420..14788)
                     /gene="recC"
                     /locus_tag="JQH51_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY6075979.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14801..15151)
                     /locus_tag="JQH51_001600"
     CDS             complement(14801..15151)
                     /locus_tag="JQH51_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY6075980.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15136..15543)
                     /locus_tag="JQH51_001601"
     CDS             complement(15136..15543)
                     /locus_tag="JQH51_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY6075981.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15540..16103)
                     /locus_tag="JQH51_001602"
     CDS             complement(15540..16103)
                     /locus_tag="JQH51_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY6075982.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16094..16564)
                     /locus_tag="JQH51_001603"
                     /pseudo
     CDS             complement(16094..16564)
                     /locus_tag="JQH51_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16748..17542)
                     /gene="thyA"
                     /locus_tag="JQH51_001604"
     CDS             complement(16748..17542)
                     /gene="thyA"
                     /locus_tag="JQH51_001604"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY6075983.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17549..18424)
                     /gene="lgt"
                     /locus_tag="JQH51_001605"
     CDS             complement(17549..18424)
                     /gene="lgt"
                     /locus_tag="JQH51_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY6075984.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18575..20821)
                     /gene="ptsP"
                     /locus_tag="JQH51_001606"
     CDS             complement(18575..20821)
                     /gene="ptsP"
                     /locus_tag="JQH51_001606"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY6075985.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20834..21364)
                     /gene="rppH"
                     /locus_tag="JQH51_001607"
     CDS             complement(20834..21364)
                     /gene="rppH"
                     /locus_tag="JQH51_001607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY6075986.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22049..22738
                     /gene="mutH"
                     /locus_tag="JQH51_001608"
     CDS             22049..22738
                     /gene="mutH"
                     /locus_tag="JQH51_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY6075987.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22807..23520
                     /locus_tag="JQH51_001609"
     CDS             22807..23520
                     /locus_tag="JQH51_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY6075988.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23658..23876
                     /gene="ygdR"
                     /locus_tag="JQH51_001610"
     CDS             23658..23876
                     /gene="ygdR"
                     /locus_tag="JQH51_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY6075989.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23984..25024
                     /locus_tag="JQH51_001611"
     CDS             23984..25024
                     /locus_tag="JQH51_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY6075990.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25057..26247)
                     /gene="lplT"
                     /locus_tag="JQH51_001612"
     CDS             complement(25057..26247)
                     /gene="lplT"
                     /locus_tag="JQH51_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY6075991.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26240..28399)
                     /gene="aas"
                     /locus_tag="JQH51_001613"
     CDS             complement(26240..28399)
                     /gene="aas"
                     /locus_tag="JQH51_001613"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY6075992.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28985..30016
                     /gene="galR"
                     /locus_tag="JQH51_001614"
     CDS             28985..30016
                     /gene="galR"
                     /locus_tag="JQH51_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY6075993.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30023..31285)
                     /gene="lysA"
                     /locus_tag="JQH51_001615"
     CDS             complement(30023..31285)
                     /gene="lysA"
                     /locus_tag="JQH51_001615"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY6075994.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31407..32342
                     /gene="lysR"
                     /locus_tag="JQH51_001616"
     CDS             31407..32342
                     /gene="lysR"
                     /locus_tag="JQH51_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY6075995.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32329..33021)
                     /gene="ygeA"
                     /locus_tag="JQH51_001617"
     CDS             complement(32329..33021)
                     /gene="ygeA"
                     /locus_tag="JQH51_001617"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY6075996.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33150..34568)
                     /locus_tag="JQH51_001618"
     CDS             complement(33150..34568)
                     /locus_tag="JQH51_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6075997.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34883..35644)
                     /gene="kduD"
                     /locus_tag="JQH51_001619"
     CDS             complement(34883..35644)
                     /gene="kduD"
                     /locus_tag="JQH51_001619"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY6075998.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35674..36510)
                     /gene="kduI"
                     /locus_tag="JQH51_001620"
     CDS             complement(35674..36510)
                     /gene="kduI"
                     /locus_tag="JQH51_001620"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY6075999.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36797..37978)
                     /locus_tag="JQH51_001621"
     CDS             complement(36797..37978)
                     /locus_tag="JQH51_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY6076000.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPVRQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38344..39462
                     /locus_tag="JQH51_001622"
     CDS             38344..39462
                     /locus_tag="JQH51_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6076001.1"
                     /translation="MVLFITALVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYY
                     GKKIGNLITTLYFIAFFVVVLIYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILN
                     LIFLMGRHATIRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWIS
                     IPVMVFAFSHTPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLS
                     IPSSYIEAAKEEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGAT
                     EVVKTTLQQVGVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILF
                     IMPTLSTYLIPALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT         9936 a        10829 c         9709 g         9556 t
ORIGIN      
        1 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac
       61 gggtgttttt ttgtttctga tctcccataa aaaaagcgcc attcagcgcc tttttatcat
      121 ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc aagaatagac
      181 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg
      241 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
      301 gcctgttcaa cttgattttt atgcagcttg ttgatttttc cgagcttatt cagtaccgct
      361 ttaccaaact taaggctgtc agcaatagtc agcgactgca ccatatcgaa catggtgtgg
      421 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
      481 tgtgccagat attttgccgc agtactggtt gcgcctttgg ttgagagcgc aaacaccgag
      541 gaaccgctcg gctgacgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
      601 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
      661 gtcatgtaca ccttcatatt gccctcttcc tcgatcagag agcgcaaacg gcgagctatt
      721 tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
      781 ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt acccggttgt
      841 ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
      901 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
      961 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
     1021 aattcaaaaa ccatgcgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
     1081 aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt caggtttaca
     1141 tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt atatttcagc
     1201 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
     1261 acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca catagcgccc
     1321 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
     1381 agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga aactttagcg
     1441 aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt cacggcatta
     1501 ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
     1561 aaaatacaat aatttcaaat aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa
     1621 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
     1681 cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
     1741 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
     1801 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
     1861 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
     1921 atattactgc tcgcctgtca atgagtctca ataacacgcc gctgcagggc gcgcatatca
     1981 acgtcgttag tggcaatttt attattgccc agccgctggg cgtcgatgac ggcgtggatt
     2041 actgccatag cgggcgtatc cggcggattg atgaagacgc gctccatcgt caactggaaa
     2101 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
     2161 tgacctcgga agagattgcc actcaactag ccatcaaact gaaagctgaa aagatgattg
     2221 gtttttgctc ttcccagggc gttactaatg acgacggtga tattgtctcc gaacttttcc
     2281 ctaacgaagc gcaagcacgg gtagaagccc aggaagagaa aggcgattac aactccggta
     2341 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgc cgctgtcatt
     2401 taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg
     2461 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
     2521 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg cgccgttctc
     2581 gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagcgcgat aacacgacta
     2641 ttgcctgcgc cgcgctctat ccattcccgg aagagaagat tggggaaatg gcctgtgtgg
     2701 cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa cgcattgccg
     2761 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
     2821 ggttccagga acgtggattt accccggtgg atattgattt actgcccgag agcaaaaagc
     2881 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg taagcgatga
     2941 aaatcaccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt acgtttattc
     3001 ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg cgattgccgc
     3061 acttaatata cgctcatcgg catacagcga cagacgacga cgcgcgcggg tcaccgcggt
     3121 gtataccagc tctcgcgtta ctaccggcgt gcgttggctc ggtaaaatca acgccgcatg
     3181 gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg
     3241 caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc agacgcgcgt
     3301 cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa gcgcgctgtc
     3361 attacgggca atcatcaccg gtcggccttc gtaccaacga gagtgcggat gacgatgaat
     3421 tttgcgcttc tgttgcataa actgttcaat tcgctcattc agtccagcca cgccaaacgg
     3481 cccttcccgc agggcgcaca aaagctggta ctcattgaac gcctgaatga ttaaatccgg
     3541 ctcggcacgc gcttgcagca gatccagata acgtccgtaa cccgcaagag cttcctcaag
     3601 catcgcaata taatcttcgc cgctctgtaa aagtcgtttt tcgatatcag taaaatcctg
     3661 ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag ctaactgacc
     3721 aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac tgtcgcgcaa
     3781 agatgccgct tctgtgccgg ttcctgccgg aacgtgagtc cccgtcaggc ggcttagctg
     3841 cctggcacgc tcggcggtaa agcccgcgtt ggcataagcg cagatatcgc ccagcacagc
     3901 cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc gcgcatgatc
     3961 tggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg cttcatctac
     4021 caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct ggctacccgg
     4081 ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc
     4141 atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc gcgcggcagc
     4201 tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca tttgaattaa
     4261 cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc cggaaatcac
     4321 cgagatccgc cgcgtcagcg ccactgccgc tgcaactttt tgccagttaa tttcatcgct
     4381 tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct caatggtatg
     4441 attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc gattcaaata
     4501 aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga ccgcttgaga
     4561 agccagcaag cattcttccc aattttgtag ctcaccgatt tcactgacac aggtcgccaa
     4621 cagcggattc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc
     4681 tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt catctcccgc
     4741 cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca cagcttccag
     4801 taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa catctcatcc
     4861 atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct
     4921 ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgaagatc atagtcgtaa
     4981 tcagcaatgc gatggcgcag ataacgatgc agcgccaggg tataaagctg atattgcaga
     5041 tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct
     5101 tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg gcggaatacc
     5161 aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg cgggcagcca
     5221 gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat gagcggttcg
     5281 ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag ctgactcagg
     5341 ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc ggtcaacacc
     5401 ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca gttcgggtca
     5461 accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc cggtgacgca
     5521 ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg
     5581 gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg ctgctgcaag
     5641 ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc
     5701 agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt ttgtgccgtt
     5761 tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc tgccgcatct
     5821 tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct
     5881 tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg ccaaaccgaa
     5941 cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac ctccgcgagg
     6001 tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg gcgatcgtga
     6061 taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac cagcggatat
     6121 tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc actttcgagg
     6181 cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga taaccagcgt
     6241 accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc gctgatatgc
     6301 aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta
     6361 cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca gatttgccga
     6421 taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt cagcgtttca
     6481 atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag ggtgttctca
     6541 cgttcgggcg tcatcaccgc ctgcaacaac caaagcattt cctgcgcttc cagagtttca
     6601 aaaacactgt cgcggttcga aaggtaaacg gaagggattt ccagcaacgt taaggcatcg
     6661 cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga agcacgcacc
     6721 ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc ttgtagccag
     6781 tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc aacgccgcag
     6841 ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt aaatacaaaa
     6901 cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc gcgaaacatg
     6961 aacgtgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc aggtgcggag
     7021 cgccagttgg tgtctaaagt gtagtgggca tgaacttcac tacgcgcctt catataagtg
     7081 aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat
     7141 gcggtttccg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg ggggtcggta
     7201 tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc
     7261 tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc aaaacccaat
     7321 tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg
     7381 atcaacagat cgcggatcga caatggttct gcaagcagtt gatcgatcgc ctcaaacagt
     7441 ggatgtcgcg gggtttcccc cccggccttc gtgcgatctt ctaagaaacg ctgggagaat
     7501 ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca ggcgctgatc
     7561 ttttcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat accagaagat
     7621 tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac cgtatcaata
     7681 cgcgccacaa tttgcgcgtg gcgggaagcc agcgtttcat catcgggcgg cggtgctttg
     7741 ataaccggcg gttcgccttg cagataacga ttaatatcgc gcagcaacgc ctgcggccct
     7801 ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca gtggcgacgc
     7861 cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag ctgctgctca
     7921 aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata
     7981 gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg cgcggcttgc
     8041 gctttatcgt cgatctcttc cagcaggcgt tcgtacagtg gattgtcggt ggtttcacgc
     8101 agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa ttctgccgtg
     8161 gcagcctcgg taaaggtgac caccagcagt tcttcaacga tcagcgggcg gggaaaggcg
     8221 gcggaaccgc ctagtccaag taacaggcgc aaatagagcg ccgcaatcgt aaaggttttg
     8281 cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg caaaggatct
     8341 agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt gctgcaacgc
     8401 gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt tcccgttctg
     8461 gctgccggaa atctgcgaca gaatagccat gccttgcggc tcgaccaccg cctgatggaa
     8521 gaaatcagca agtttttgcg gcgtcagcag ttttatctgg gccacgattt tatcacgcga
     8581 atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt cttcgccgag
     8641 cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg caaactcatc
     8701 tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt aacgctccca
     8761 caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc actgacgccc
     8821 cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac gcaactgatt
     8881 gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc tggtgtattc
     8941 atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt taccggcttt
     9001 ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac accactctga
     9061 accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct gggcctcggt
     9121 catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt cgcgataggc
     9181 cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc gcgagaagta
     9241 cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc
     9301 tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgct cttccgtagc
     9361 ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac gctgggtgta
     9421 accattagca ttaatcataa ggccgttgtt agcgttggtg gaaaaactta tgccaccaac
     9481 cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat tgagcgcaaa
     9541 catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca ggctgacatc
     9601 agctttgggc tcgctggcaa aataacggct tggcgcatac accacgcgca gattcgactc
     9661 atcaacaatc agctctggat ggtcgtattt cttctctgac ttaatcagcg agaaatcatc
     9721 aggaatataa gggttaagct ctggcaaaga gagcgcaatg tcggcggctt ttttctgcca
     9781 gtcggcgaaa gtttgtgcgc tgattttatc gacctgatac ggcgcatcga caaagtaagc
     9841 cgttttgttg tgcggctctt tcgggctgat ataccagata cgcgcattct gcggcgtcat
     9901 catcgccaga cgttccttta ctgctttagc atcgtaccga tcggcaatat tgactgcatc
     9961 cagcgtatgc tcaacaggaa cgcgaatcat ggtatctgcc agccattcga cgtaatccat
    10021 atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca gttcatcgaa
    10081 gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa ttgccgccac
    10141 aacctgatcg cgattagcca ggcctttatc agttaaagac gcagagatcg ctaatacgcc
    10201 gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta atccctgctt
    10261 ttgcagccag tcagaaagtg tacctgggct gcgattgcca atcagatagg taatcaattc
    10321 atcggtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa cgcgcaacac
    10381 tttacgcggc agcgcaggga cgtaatgaat gataatgccc ttttgcgcgt cggtgactac
    10441 cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc
    10501 cgccattttt gccaactccg gcagcggttt attactgtaa ataaccgcct tcatcaaatt
    10561 ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat taccaggttt
    10621 gtcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg ccgggttaat
    10681 ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc
    10741 agcgttcact gcattacgct cacgttcggc gtatttcttg tcgagcaagg gttctgcaat
    10801 agcatcggcc aggcggtcta ccgcaccggg caaggcgtcg ttctcaactt ccagatagaa
    10861 agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt tgagatattc
    10921 ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat gttcaaggta
    10981 atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca ctaccagcgc
    11041 cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac cgttatccag
    11101 acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt cctgaatcgg
    11161 ctgccatccc gtttctgcct gacttaatgg tgcccagagg gcaacaaaca acaataatgc
    11221 tttgaaccag gtgctgcggg gcattcacgg acctcataat caactttatt ttctggacag
    11281 attcaacagg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca cgagccagcc
    11341 tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc agatagattg
    11401 cgcaattttt atacaacact catgactgat taaagcgaaa cagcggtaac aggaaacgtt
    11461 gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag agccgttgat
    11521 accagatatc atcaccttcg ccacgcacca tcatgttgcc ttcgtaagcc tgaaggaatt
    11581 tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat
    11641 aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc gcggacattc
    11701 cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg
    11761 gcggaaaacg ccactcaccg tctttgcgta gaaaaaggcg actttcgcca ttaccaccgc
    11821 tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca cttaataaag
    11881 agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca gttatctgca
    11941 caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag gcaatgactc
    12001 tgtcggcaag ctgctgcatc tcctggcact gtgtttccca gaaaatttca ccaaaagcgc
    12061 catacggtaa atcccctgcc gcccggaagc ggcggaacaa gcgttcggca tcatcctgct
    12121 caaccagtgc attcaataac tgctgattga tttgataacg gctaagtcct tccagaataa
    12181 atggctcggt gtctgggatt tcgctgtctt cagtatggaa gttcacctgc aaacgcatct
    12241 ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc ggcacggttt
    12301 ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca gcctggctgg
    12361 ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctggtag ttttgcggat
    12421 caaacggcat ccgggtatgg aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat
    12481 cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag tcgatcagtt
    12541 cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc ccgatatagc
    12601 tgatatagag tttttgctgc gcggaaatta acgcttccag gaacaggtag cggtcgtcat
    12661 cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag cccaatggcg
    12721 caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa
    12781 ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga
    12841 tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt
    12901 cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt tcgatcagcg
    12961 tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gttgagcata tcgcgacaaa
    13021 ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag atgtttagct
    13081 gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc gattcatcat
    13141 aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc agcaacatac
    13201 gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt
    13261 tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt aaataacgca
    13321 gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagc agcgccagca
    13381 catcctctga cacaaagcga ctgtcaggca gtgataacag gctgataaac gcttccagta
    13441 ccggatgtga ctgccgcgca cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag
    13501 gtgcgctacc aaacactgcc tgaataaacg gactgtagct gtcgatatca gccaccatca
    13561 cgatgatgtc gcgcggagta agtgtcgggg cttcctccag catcgccagc aagcgatcgt
    13621 gtaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaatgtgata ctgctatcca
    13681 gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc acacccgcaa
    13741 cggcgcggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg ttatctggcg
    13801 tcacatcgac aaaagcgtcc agctcctggc tgctctccag gtcagaaagg agataaatgt
    13861 agtcgcgccc aagcttcccc catgaggcca gcagcgagtt gccgacatcc tgttcaccat
    13921 cgctgttaaa gagctgtccg gcattttcgc tatcgcgaaa tagcggcaat tcgcgatctt
    13981 caaaactgtg tcgacgctga cgagtcagca gtttcgccag ataagcagga tctttaatat
    14041 cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata tgtttaccca
    14101 gcgcctggag cgcctggaga taaacaggcg gtaacgcgga aataccgcat ataaagacgc
    14161 gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata aagcgctgat
    14221 agagattcgc gcggtgccag cgcggttgtc cgagttcatg ggtatattcc accagcgcct
    14281 tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt
    14341 cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg tccgccgctt
    14401 ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca
    14461 gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc cagctcatgc
    14521 tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg aacatatccc
    14581 agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aataccaaac ttttgcgaca
    14641 gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc tctggttcga
    14701 aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc agcacgtcca
    14761 gacgattgga atggtagacc cttaacatag cggctcctga ctactgacaa ttcgggcaat
    14821 gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga
    14881 cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg
    14941 cgcgcagttg cgtttgctgc caaccatgcc gccagagctt ttggtactgg tttctgctgg
    15001 cgagactgtt cattgatgtt cgctggatac ccgataacgc agtgacaatc atcaccatca
    15061 gcaccatcag caccatcagc accatcagca ccatcgcaac cattacctcc ggcaggctaa
    15121 agccttgttg attcttcagg gaagctgaca taatgccccc tctttcagcg gacaaaaatc
    15181 gctccagccg cgtggcgaaa aaacaatttt tccatcaatg acttcgcctg ttcgccacaa
    15241 cgaaacgcct tcataaccgg caatcaataa ggcttcatta tctgccagta aacgcaaaca
    15301 aacctgggca tcggttccag cgtactgcga gcactgaact gctggctgcg tctgccagga
    15361 gtgcattttt ccccacgcca gcgccgactg aacgatggcc tggcggcgca atgactgact
    15421 ttccatgctc acgcgagagg caaaactgcg atcctgctga ctcattcctt gcaatagcaa
    15481 gctacccaaa accagcagca tcaggaccag agccagtgac gaaacacctt tttcgcggtt
    15541 cacaggttga atcctgtcac gctatagctg gcatccacca cggtttgcgg ttcagactta
    15601 ctggcagcac gcatattaac cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg
    15661 acctgaaaag tgtcgataat gatggcatcc ggattagtga ctttatccca gcccttacct
    15721 tcacaggatg tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg aaatccaatc
    15781 tggtcggact cttttaccgg ttcgcgatcc cagataccgt tactgttcgc atcccactgc
    15841 acaatgatac agtcaccctg tccgacaatt tccagccctt cgccggtaca gctgccatga
    15901 caataacccg ccctctggag atgcttcgcg acggtaaata cccgcagcca gatttcatct
    15961 tccagtgcca gcttacgagt gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt
    16021 gcagccccca gcaacaatac gctactgatc gccatagcaa tcaacacttc cagcagagaa
    16081 aaaccttgct cttttacagg catccttctg tttctccttg ctgataaagc cggagtcttc
    16141 cccacggcga aaccaccagc caccactcgc ccgttgagtt tttgaagcga atatgcacgg
    16201 cccatgcggt attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga
    16261 cttcgggcca gcgtggcaca aagaccaatg gtgaactgcc atgacaggta ttggccccag
    16321 cagcggaact cacaaggcac cataacgtcc cctccctgat aacgctgata ctgtggtcgc
    16381 ggttatgcca gttggcatct tcacgtaaat agagcaaata gtcccgcgcc tagctggcgg
    16441 tttgccaaag ccgttgcgac tgctgccagt attgccagcc atagagtcca cttgcgctta
    16501 gcatgaccaa aatcagcatc gcgaccagcg tttcaatcag cgtataacca cgttgtgttt
    16561 tcatgccggc agtatggagc gaggagaaaa aaagacgagg gccagtttct atttcttcgg
    16621 cgcatcttcc ggactattta cgccgttgca ggacgttgca aaatttcggg aaggcgtctc
    16681 gaagaattta acggagggta aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt
    16741 ttcgtaatta gatagccacc ggcgctttaa cgcccggatg tggatcgtag ccttcaatct
    16801 caaagtcttc gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg
    16861 gcagcggacg cggttcgcgg cttaattgca gatgagtttg atccatatgg ttgctgtaca
    16921 ggtgcgtatc gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca
    16981 tcatatgcac cagtaacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac
    17041 aggagcgctg ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg
    17101 catggcacgg tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa
    17161 tgcggcgcga atccgggtcg tttttcagct ggttcagtac cgtagtaatc tggtcaatat
    17221 gacgaccatc tggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc
    17281 cattttcatc ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt
    17341 tagtatcgcc ctgcaggaac cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt
    17401 tggttgtcac cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg
    17461 aaagcgttcc ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca
    17521 tcagttctaa atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat
    17581 atgcccagac catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca
    17641 tgctgatgta ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa
    17701 caatgatgcg aaacgcgccg tagccaatca ggaacaagcc tgagacagct cccattgggc
    17761 gcggtttacg aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagtt
    17821 cgtaaagctg tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact
    17881 gcgggttggt ttgcagcagc aaaatatctt ctgtacggga gcctgggaac agcatggcaa
    17941 acgggaagtt cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc
    18001 cggcaccaag accaaacgga atgagtggtg cgataaaatc agagacctgg aagaaggaac
    18061 gtttagtacg gcgggcgaag ataatcatca ccacgataac gccaatcagg ccgccgtgga
    18121 aagacatgcc gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga
    18181 aattgtagaa cagaacataa ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat
    18241 agagtaagtt ttcaacttca tttttggtcc agccactgcc cggacgattc gcccgacgtg
    18301 ttgccagcca cattgcaaaa atgaacccca ccagatacat caggccgtac cagtgaagcg
    18361 ccacgggtcc tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg
    18421 tcatctgtca ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg
    18481 ccgcaggttt caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc
    18541 cggataccgt taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc
    18601 ccatgccgcg acgctccata aaggctgcaa cctgatgacg aacttcggtc gccagttgcg
    18661 cttccagact acgctgcgca agattttctg cttcggcaaa atcaatgcgc cgcagcaggt
    18721 attttacccg cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga
    18781 ggattgccac gcacatggga tcgcccgcca tttcaccgca caaacgaaga tcgataccat
    18841 gtatttccgc ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt
    18901 cgtaaatgtt cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat
    18961 cgttggtgcc aacagagatg aaatcgaccc tctttgccag atgcggcagc ataaatacca
    19021 ttgacggcac ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct
    19081 cctcgacttc acgtccggca cgttcaatca ggcggcgtgc ttcgtcaact tcatcgaggc
    19141 tggtgaccat cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg
    19201 cccgcacctg gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac
    19261 ccaggcatgg attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat
    19321 ccagcgtacg caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg
    19381 ccacctgttc ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat
    19441 aaagaccgat gccatcaata cagctgccca gtttttcttc atgttccggg cttaaaccag
    19501 cattgagcat gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat
    19561 cttccgccag acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca
    19621 gtaccggctc cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac
    19681 gatgcagcac cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta
    19741 ccatgatcgc agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat
    19801 cctggggcag ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt
    19861 ccggccaggc gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca
    19921 gcgcacgtaa atcaccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt
    19981 cggcaaattt ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct
    20041 cggcaaacaa ttcgcgacgc agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa
    20101 tagccgccgt ttctttttgt gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt
    20161 ttgccgcttc ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg
    20221 atgcctgata cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg
    20281 ccacaccagg tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaggg
    20341 cagtcaactg cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg
    20401 attcttcact ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca
    20461 actggcgacg ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg
    20521 aggggatgta tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca
    20581 gcctgccaac caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg
    20641 gttttttcag cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt
    20701 agaccgaaca gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg
    20761 cctcattcag gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca
    20821 taattggcgt gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg
    20881 tattttcctg cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat
    20941 cacgtttaaa tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt
    21001 caaactctgg tgtactgctg gtttgcatat tgatttctgc atcgccgctt accagctgca
    21061 agagaaacca tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac
    21121 gtttcggtaa tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc
    21181 ggcttaatcc tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg
    21241 ggttgattcc gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta
    21301 cctgcccctg gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa
    21361 tcaccggact acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaggaag
    21421 acggtaaacc actctctgca gggcattaca cactaataac aattgaataa ctcacagtta
    21481 tgtgcagagt tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg
    21541 atagagttgt gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc
    21601 cgatatagac attttaaaat attcattatt agtcaatgca ttacatgtaa tttgagtaat
    21661 gaaaaagagt aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa
    21721 ccaatgccgt gtttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact
    21781 ggagtttcgt cgcacgtcaa ggctgtaaat gaaaacagta gtggaggttt ttcacagtta
    21841 tcccagcttt ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg
    21901 gaaaacctat tgcagtgttg cgcaactcga ttaccggcag cctaaaaagc aatataaatc
    21961 agagaattga acaacacatg tggaaaaagt tacactgcga atattcggca cataattgct
    22021 gtttgttttt taaccaaggt atcatgacat gtcccaacct cgcccactgc tctctcctcc
    22081 cgaaactgaa gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga
    22141 actggcggca cttgacgggc tggttacgcc agagaattta aaacgcgata agggctggat
    22201 tggcgtgtta ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt
    22261 tgctgctctg ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga
    22321 aacaacattc gtttgtgttg cccggttaac gggtaatagc ggggtgacct gggaaaccag
    22381 ccatgtgcgc cacaagctca aacgcgtact gtggataccg gttgaaggcg agcgcagcat
    22441 cccgctggcg cagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga
    22501 ccgacagcta cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga
    22561 gcggatcacc gctcgtcacg gggaatattt acagataaga ccgaaagcag cgaatgcgaa
    22621 agcgcttacc gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt
    22681 ttatttgaag aagaatttca ccagtgcact actggcccgt cattttctga tccagtagcc
    22741 atcgctttga cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct
    22801 ttttagatgt tatttgcatg gataaccgat cctaacgctt ggcttgcgct cggtacgctg
    22861 acgctgctgg agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca
    22921 aagcttccca cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt
    22981 atgcgtctgg cgctgctggc atcaatctct tgggttacgc gcctgacaaa tccgcttttt
    23041 acaatattca gtcaggaaat ttctgcccgt gatttgattc tgcttctggg tggcttgttc
    23101 cttatctgga aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg
    23161 aaaacacgcg tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc
    23221 ttcagcctcg actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg
    23281 gcagccgtgg tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt
    23341 gtcgaacgcc atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt
    23401 accctgattc tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg
    23461 ttcttctcta ttgcggttga aagcctcaac ttgattcgca acaaaaagaa tccgctctga
    23521 tactccgtac gctctcctgc gggagggcgt actcactcct tttaccctca cagtttaaga
    23581 ttttcctgct ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtaggc
    23641 caaatgagga taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt
    23701 ttgctgtttc cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt
    23761 tgaccgatgg aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc
    23821 aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa
    23881 taaggtcagc atccggctgg ccttaagatt tttctctttc ccttttccct tccctctgcc
    23941 atttttatat tccttatgtc gtgattataa aaaggaaacg gctatgcaat atcaccgtat
    24001 accccacagt tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca
    24061 gaacagcgaa gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct
    24121 tatcgacgtt gccgaaatgt acccagtacc tccgcgcccc gaaacgcaag ggttaaccga
    24181 aacctacgtc ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc
    24241 caaagtgagc ggaccgtcgc gcaataatga caagggcatc cgcccggatc aggcgctgga
    24301 tcggaagaat atccgcgaag cgctgcatga cagcctcaag cgcctacaga ctgattacct
    24361 cgatctttat caggtgcact ggccgcagcg cccgaccaac tgcttcggca aactcggtta
    24421 tagctggacg gattctgcgc ctgcggtttc gctgctggat acgctggacg cactggcaga
    24481 gtaccaacgc gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt
    24541 aatgcgctac ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcagaa
    24601 cccttacagt ctgttaaacc gcagttttga agtaggtctg gcagaagtca gccagtatga
    24661 aggggtcgaa ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct
    24721 caatggtgca aaacccgctg gcgcacgtaa tacgctcttt agtcggttca cccgctatag
    24781 cggtgagcaa acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatggcct
    24841 tgatcctgct cagatggcgc tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct
    24901 gctgggcgca accacgatgg atcagctgaa aactaacatc gaaagtttgc atctggagtt
    24961 aagcgaagac gtattagctg aaattgaagc ggtacatcag gtttatactt atcctgcacc
    25021 ataaagatta acgccggtta aaaccggcgt taaatattaa tgacggcgct gccagatcca
    25081 cagcgccgtt attgccagcg caaatagcgc accgaagcca atgccaatgg gcacgaccgg
    25141 gatgcctacc attaccgcca gcgagtaaat gcccagcatc aacaacatgg cgctgttttc
    25201 gccaaggttt tgtactgcaa tcgcattccc cgccccgacg ctttttttac cccgctcctg
    25261 tagcaacgca ttgagcggaa cgacaaaaaa gccccccagc acgccaatca gcatcaacaa
    25321 ggcataggct ggcagcagct cgtgttgcag ggagaaaatc ggtaccacca cgccaatcaa
    25381 aatcccggct ggcatacagc gtgacacggt ttccagcgta accagcttcg ctgccgcacc
    25441 tgcgccaacc acgataccaa tcgctaccat cgcgttgaga taggtcggcg tagcgttatc
    25501 ggtaatgccc agcgccaccg gtacccacag caccaacagg aaacgcagcg tgacacccgc
    25561 tccccagaat aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc accatagcga
    25621 ggtgcaggca ttcaggaaac tgcgggtcat gttgatgaga ttccaggact gccccggacg
    25681 cgccgccagt ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca
    25741 tgcggccaga gcgaccagga catgccagtc agccagcaca ccaccggcta cggaaccgag
    25801 taaaatcgcc gctatggcag aagcttccat taaaccgtta gcttttacta acttactacc
    25861 cgtggttaat tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc
    25921 caccagcgta tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag
    25981 cttcaggccg ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc
    26041 gacaaacggc gccaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct
    26101 ccactccgga tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gcgcgttatc
    26161 gccaaacgca gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga
    26221 agtgttagtg tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca
    26281 acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat
    26341 cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc
    26401 gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct
    26461 taatcgcagt ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt
    26521 ccagcgacac catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca
    26581 caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt
    26641 cgccgcgaac attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat
    26701 agccgttcat tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga
    26761 ccgacaacag gcgtgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg
    26821 ccatcggtac gttgatagaa acgacaggcg cgcattcggt cacaccgtag ccttcaagga
    26881 tgcgcaggcc aaatttatcc tgccaaagct gtttggtact ttcttgtagt ttttctgcgc
    26941 ctgccaccac atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac
    27001 cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg gtcatacacc aagtccggca
    27061 caatgcggta atgcagcggg cttggataaa ggaacacttc tgcacctgta agcagtggcg
    27121 taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg taatgccgac ataaagcgat
    27181 cgttggtggt gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat
    27241 ggctatggac gacgcctttt ggatggcctt cagaaccgga ggtaaagagg atcagcgcct
    27301 cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat cagcaaatga gcgaagatcc
    27361 atactttgtc ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct
    27421 gagtaagttg ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga
    27481 agatggtttt gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt
    27541 agttcatcat tgcgggaatg cggcgacgcg cgatggcccc aaaaatcact gccgcactga
    27601 tgcctgcatt gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga
    27661 tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga
    27721 cgtcttcgac acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt
    27781 acagagtttc acgcgggcgc accgccatac gcgcttccat cattatttga tgcagcattt
    27841 cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc cggcatcgcc acctgcgttg
    27901 gaggcaaaat atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc
    27961 ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact
    28021 tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag cgagcctgta gtggtgatgc
    28081 gtccttcagg gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa
    28141 tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca
    28201 tataccactg ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca
    28261 gtccaagcaa aatgccatca ataaaagaga cgtgattagg cgtaattagt acgcgctcgc
    28321 ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc
    28381 gaaaaaagct aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg
    28441 gagattacac gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg
    28501 catccgcgca ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa
    28561 tcaatacctc tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg
    28621 caaaggaatg aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga
    28681 tgaccggcaa aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaacac
    28741 gaagttgact tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac
    28801 gccaccagcg gtcaatcgca acagcgtttc gcaaagtgta accaaaggtt tgcaatctct
    28861 ttttttgtct tacttatttg cgtttggctc acattcccac gatgaaaaca cgccacccct
    28921 tgaaccaacg ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat
    28981 tttcatggcg accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc
    29041 ccgcgtcatt aataattcac ccaaagccag cgaagcttcc cggctggctg tgcatagtgc
    29101 aatggagtct cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac
    29161 tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa
    29221 agcggtcgaa caggtggctt atcacaccgg taatttctta ttgattggca acggttacca
    29281 caacgaacaa aaagagcgtc aggctattga gcaactgatc cgccatcgct gtgctgcgtt
    29341 ggtcgtccat gccaaaatga tcccagatgc cgatttagcc tcattaatga aacaaatgcc
    29401 cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctcttga
    29461 cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc atacccgcat
    29521 tggttatctg tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta
    29581 cgatgccctt gctgaaagtg gtattccggc caatgatcgg ctggtgacat ttggcgaacc
    29641 agacgaaagc ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac
    29701 tgcggtagcc tgttataacg attcaatggc ggcgggcgcg atgggcgttc tcaatgataa
    29761 tggtattgat gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg
    29821 ctatgtgcgt ccgcgcctga ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc
    29881 tgccgaactg gctttggcac tggcggataa tcgccctctc ccggaaatta ctaatgtatt
    29941 tagtccgacg ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca
    30001 tgcaaccagc gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct
    30061 ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac
    30121 ggctattgta gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat
    30181 aatcacctgc cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg
    30241 taaagacatc gcccgattca cataacggcc cggcgacgac ggtttccacc gttggcgcgt
    30301 gttccagaga acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg
    30361 ccgggcgcat cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt
    30421 gtttgacgct ccgcacctga gtaattaata cgccagactg cgctaccagg aagcgacccg
    30481 gttcaatttc cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat
    30541 tccacagacc ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag
    30601 aaagcccacc gcccgcagaa atagcctgta aatcctgacc gaattcgata acctgacgca
    30661 ccatagcacc acacacctgc tccagatggg cataatcaac gccagaacca atgtgcatgt
    30721 gaatgccgac tagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg
    30781 tgtaccagat accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt
    30841 gaccaaaccc cggattaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt
    30901 ggtcgagcgt atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa
    30961 gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat
    31021 tgtaacccgc cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc
    31081 cctgctcacg cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca
    31141 ccacttcaaa ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc
    31201 acaccgggca gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat
    31261 cggtgctgaa cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg
    31321 ccacatcata aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg
    31381 gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt
    31441 ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac
    31501 agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg
    31561 agcgcgtacg tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc
    31621 aacgatcctg gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc
    31681 gccagggaga actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc
    31741 tcctgcaacc ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat
    31801 caccgctact tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac
    31861 tccatacgcc tacgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt
    31921 tacctccggg tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg
    31981 agaattacat cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta
    32041 ctgaacatca ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg
    32101 caatggtacg ggcgggggta ggtatttcgg tggttaaccc gctcaccgca ctagattatg
    32161 cggcaagcgg tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga
    32221 tccgccccct gcaccgcccg tcatcagcgc tggttcaggc gtttagtggg catttacaag
    32281 cggggttacc gaaactggtc acttctcttg acgctatttt gtcgtcagct acgacagcat
    32341 aaaagcgaca gcatcctcgg catggatcgc cgcggtatca aacacaggca aaacactgcg
    32401 ctcttctggc accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg
    32461 ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc
    32521 cagacacagt tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat
    32581 aagacaattg atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt
    32641 gtaacgcgta cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt
    32701 ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt
    32761 atgcatcgta ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc
    32821 cgcctcagcc agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat
    32881 ttcatgaaaa tcgacgctat gtagcagcat ttgcgcagag tgaagcccac caagccgctg
    32941 tttaatgcct tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc
    33001 cagcaaacca attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat
    33061 aaggaacgag acaaacgcct caacggccag gtgcccaatc tctattaacg aaaaaagggc
    33121 cggatgtaca gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct
    33181 gccatcagtt tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag
    33241 aaagtaatac ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg
    33301 ccaatgctat caagcagtgt caggaaggcc gcgccgataa tcatattcga cacccagttt
    33361 gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac
    33421 aggatccaca ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg
    33481 ccaacagaga gccaggacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa
    33541 tagcccagca ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta
    33601 cgacctgctt tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc
    33661 acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata
    33721 cgcggcgcgt agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac
    33781 ataccgagaa acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgccc
    33841 tgttttaact tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta
    33901 tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag
    33961 cgcgggctat ttggcaggaa gaccaccaga ataatcagca gaactgctgg taaagcaaga
    34021 acccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc
    34081 agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt
    34141 tcacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg
    34201 cccagcacca cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata
    34261 gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac
    34321 gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc
    34381 cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg
    34441 attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac
    34501 atattcatac gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata
    34561 gtaaccattt tttcctgcca gcagagagta agacattgtg aaaaaatacg tgaacaattc
    34621 acacaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta
    34681 tgtgatggat attccaattt taaaattaag ttgaattatt gagattatta ttaacctcct
    34741 aattttaaag cagataaaat tcataaagtt cattaattga taattgatat ggattatttc
    34801 ataaccatga tatggattat gatgatctag agatataaaa aaccctgcca tgcggcaggg
    34861 tcataaaagt aagaggaatg aattaacgcg ccagccaacc gccatccaca gcaatggtat
    34921 aaccatttac ataatctgaa gcgctggagg caagaaacac taccggcccc atcaggtcac
    34981 tcggcagtcc ccaacggcca gctggaatgc gatcgagaat ttccgcgcta cgttgttcat
    35041 ctgcccgcag ttgttgggta ttgttggtcg ccatgtaacc cggagctatc gcattaacat
    35101 taatgttgtg tttagcccat tcgtttgcca tcaatcgcgt cacacccatc acgccgcttt
    35161 ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt gacgcgatat
    35221 tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc tgagacatga
    35281 agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg ctgaactcga
    35341 gagcgtcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata tgaccaaatt
    35401 cagctaccgc gcgatccagc agtccaggaa tgccatcaat ctttcgcaga tcggcggtca
    35461 ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat ggtttcagtc ggttcaacga
    35521 tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc ccctggccca
    35581 gcccagtatc acaaccagtg acgaccgcaa ctttaccttg gagagaaaat gcacttaaaa
    35641 tcataacaac accttattcg tttatgccca caactagcgc aaatctttaa cgcccacatg
    35701 gtccatatca ttaaagacct ggttttcacc gaccatgccc cagataaagg tataagcttt
    35761 ggttccgacg ccggaatgga tcgaccagct cggggagatc accgcctgct cgttatgcat
    35821 cacaatgtga cgcgtttctt gcggctgccc catcatgtgg aaaacgcagg cgtcatcatc
    35881 catattgaaa tagaaataaa cttccatccg gcgctcgtgg gtgtgacacg gcatggtgtt
    35941 ccacaagtta cctggagcca gctccgtcag ccccatactc aattgacagg tttccagtac
    36001 atccgggaca aaatatttgt taatcgtgcg acggttactg gtgaggttat cgcctaacgt
    36061 gactggagat acttcgtccg gtgtgacttt tttggtggga tacgtcgtat gcgcgggtgc
    36121 gcaattgtaa taaaacttcg ccggagtggc ggtatcaata ctggcaaaga caacttcttt
    36181 tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt tcatagcatt gaccatcgac
    36241 agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt caaggaaata
    36301 gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag ttatcggcat
    36361 aatgccgcca acaataattc ggtcaatgtg gctgtaaacc atggtgtact catcggcgac
    36421 aaataccttt tcaaccaaaa attcattgcg cagcccttgg gtatccagcg tttttgcgtg
    36481 cgcaccgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt gaagcgtgaa
    36541 aatagaaaac gatagtgaaa cactgtttcg ttttctctat aaagggatga tatgcccatg
    36601 ggctagggtt ttcaattaaa aataaaacaa tgttttattt ttaatttaag gattaaaaaa
    36661 agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa atgagggagc ccagaaaatg
    36721 tatgtcgact tgccagccat cattattcaa cagattaatg acggcagata tttatcggta
    36781 aggaggaaat gcagagctac tcgtcacgtt caatggtcaa tgccacgccc tggcccccgc
    36841 cgatacaaag cgttgccagc cctttgcggg cattacgttt caccatttca tgaaccagag
    36901 aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc gagtgcgatc gcgccaccat
    36961 tgacattgac ccgacgctca tcccactcaa gcatcttgcc aaccgaaagc gcctgtgcag
    37021 caaacgcttc attagcctcg ataagatcga catcagccaa ctgccagcct acacgctcca
    37081 ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa tgccggatct acaccaacgc
    37141 tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc
    37201 tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc gcggtcacag
    37261 aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc
    37321 gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc attaccggga
    37381 cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa
    37441 gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga ttttcggcgg
    37501 tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc agactgtcaa
    37561 ccaactggct attgccaagc tgttcaccgg tgcggctatc agtcagaaca tgtggtgcgc
    37621 ggctcatgtt ttcctggcca cctgcgatga caatatcagc ctcgccacac tgtatcgcct
    37681 gagtagccag atgcagtgct ttaagaccgg aaccgcaaac gtcattaatc gtgattgcag
    37741 aaacgctgtt aggaagacca cctttaatag ccgattgcct taccggattc tgccctgccc
    37801 ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga acgccggtac
    37861 gttctattaa cgctttcacg accagactac caagttccac ggcggaatga cccgctaacg
    37921 caccacgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg tctttcatca
    37981 caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt tatcttaatt
    38041 gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt cagagtcata
    38101 actggcacgt aaggtttgca accactaacc caccaataga tgggtagata gggcgttaat
    38161 ctcccatact taacttggtt tatggtaaat tgccctccat tttgtttaat ttgtagatga
    38221 tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtgaag tttcgcgctc
    38281 tatggcacag ccgttgatgc aggcacgcta ttccttccta ttcagttagg ttcggcaggg
    38341 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg gccacataaa
    38401 gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat aacgggcgcg
    38461 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
    38521 ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac ggaacagctg
    38581 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta
    38641 attctgaatc tcatttttct gatgggacgt catgccacta ttcgggtaat gggatttttg
    38701 gtattcccat tgattgccta tttcttattt ctttccattt accttgtcgg tagttggcaa
    38761 cctgatctat taacaaccca ggtagagttc aatcagaata cccttcacca gatatggata
    38821 tcgattcccg tgatggtttt cgcctttagc catacgccca ttatttctac gtttgccata
    38881 gacagacgtg aaaaatatgg cgaacacgct atggataaat gcaaaaaaat tatgaaagtc
    38941 gcttatctca tcatctgcat aagtgtactg ttctttgtct ttagctgcct gctttctatt
    39001 ccatcttcgt atattgaagc ggctaaagaa gaaggggtca ccattttatc ggcgctttct
    39061 atgctgccga acgccccagc atggttgtca atttccggga ttattgtcgc agtagttgcg
    39121 atgtcgaaat cattcctggg tacgtacttt ggcgttattg aaggtgccac agaggtcgtc
    39181 aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg catttaacca cgcactatca
    39241 attatgttgg tatcgctgat taccttcatt gtttgttgca ttaacccgaa cgcgatttcg
    39301 atgatttacg cgatcagcgg cccgctcatt gccatgatac ttttcatcat gcctacgctg
    39361 tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca tcggaaatct gattacgctg
    39421 atcgtgggta tcctgtgcgt atcggtaatg ttctttagct aatatccgtt gtggatggtt
    39481 caaaattgct acaattttga accattccat ttttcataat attcatttgt tattattccg
    39541 ccctatcact tctctcgtca tatcctagaa ttatcagtta gttaaacaca caaagagatc
    39601 tgaacatctt aaataagact atttaagatg cataacttag attcgcaaga tatcttctgg
    39661 tcattcaaaa acaatttgcg atatagatat tctcatacca tgagatagtt atctggacca
    39721 cgttcgacca tgcaagatta aatcagtgaa acataatcat atttaataac gagaaatgca
    39781 tttttaaata tcttaatcag aattatagca aatacagatt aatcgcaatt tttcccagca
    39841 aaattacaaa atcaaggtgt attgacatca tcaatcacca atgaaattat gcaattatat
    39901 acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct tacatgcaat
    39961 agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca gtactgcgca
    40021 aactcgtgtt
//
LOCUS       DADLEE010000030        39990 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000030 DADLEE010000000
VERSION     DADLEE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.030"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..155
                     /locus_tag="JQH51_001623"
     CDS             <1..155
                     /locus_tag="JQH51_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6076002.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /locus_tag="JQH51_001624"
     CDS             384..974
                     /locus_tag="JQH51_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6076003.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="JQH51_001625"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="JQH51_001625"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY6076004.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="JQH51_001626"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="JQH51_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="JQH51_001627"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="JQH51_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY6076005.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="JQH51_001628"
     CDS             complement(5626..7134)
                     /locus_tag="JQH51_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY6076006.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JQH51_001629"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="JQH51_001629"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY6076007.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /locus_tag="JQH51_001630"
     CDS             8609..10255
                     /locus_tag="JQH51_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY6076008.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="JQH51_001631"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="JQH51_001631"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY6076009.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JQH51_001632"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="JQH51_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY6076010.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="JQH51_001633"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="JQH51_001633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY6076011.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="JQH51_001634"
     CDS             complement(14283..15602)
                     /locus_tag="JQH51_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY6076012.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="JQH51_001635"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="JQH51_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY6076013.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="JQH51_001636"
     CDS             16699..17034
                     /locus_tag="JQH51_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY6076014.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="JQH51_001637"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="JQH51_001637"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY6076015.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="JQH51_001638"
     CDS             complement(17790..19172)
                     /locus_tag="JQH51_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6076016.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="JQH51_001639"
     CDS             complement(19358..20302)
                     /locus_tag="JQH51_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6076017.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="JQH51_001640"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="JQH51_001640"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY6076018.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="JQH51_001641"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="JQH51_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY6076019.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="JQH51_001642"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="JQH51_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY6076020.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="JQH51_001643"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="JQH51_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY6076021.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="JQH51_001644"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="JQH51_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY6076022.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="JQH51_001645"
     CDS             complement(25947..26768)
                     /locus_tag="JQH51_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY6076023.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="JQH51_001646"
     CDS             complement(26868..26951)
                     /locus_tag="JQH51_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076024.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="JQH51_001647"
     CDS             complement(27044..27379)
                     /locus_tag="JQH51_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY6076025.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="JQH51_001648"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="JQH51_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="JQH51_001649"
     CDS             29136..30047
                     /locus_tag="JQH51_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6076026.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="JQH51_001650"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="JQH51_001650"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY6076027.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="JQH51_001651"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="JQH51_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY6076028.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="JQH51_001652"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="JQH51_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY6076029.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="JQH51_001653"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="JQH51_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY6076030.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="JQH51_001654"
     CDS             complement(34283..35137)
                     /locus_tag="JQH51_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY6076031.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="JQH51_001655"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="JQH51_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY6076032.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="JQH51_001656"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="JQH51_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..39032)
                     /locus_tag="JQH51_001657"
     CDS             complement(37866..39032)
                     /locus_tag="JQH51_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY6076033.1"
                     /translation="MLIPIGRPRERTGWIYSVIMINVSHIRTLSSIESTTSSPSIRLA
                     MESIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMT
                     VEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRT
                     QEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTP
                     SGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKS
                     RTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRIL
                     PGVSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39990
                     /locus_tag="JQH51_001658"
     CDS             38958..>39990
                     /locus_tag="JQH51_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6076034.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10164 a         9700 c        10242 g         9884 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagtctg
//
LOCUS       DADLEE010000031        38786 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000031 DADLEE010000000
VERSION     DADLEE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38786
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.031"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JQH51_001659"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JQH51_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY6076035.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="JQH51_001660"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="JQH51_001660"
                     /inference="COORDINATES: protein motif:HMM:NF019659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY6076036.1"
                     /translation="MTRVQFKHHHHPD"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="JQH51_001661"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="JQH51_001661"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY6076037.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="JQH51_001662"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="JQH51_001662"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY6076038.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="JQH51_001663"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="JQH51_001663"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY6076039.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="JQH51_001664"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="JQH51_001664"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY6076040.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="JQH51_001665"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="JQH51_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY6076041.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="JQH51_001666"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="JQH51_001666"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY6076042.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="JQH51_001667"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="JQH51_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY6076043.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="JQH51_001668"
     CDS             7139..7750
                     /locus_tag="JQH51_001668"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY6076044.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="JQH51_001669"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="JQH51_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY6076045.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="JQH51_001670"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="JQH51_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="JQH51_001671"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="JQH51_001671"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY6076046.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="JQH51_001672"
     CDS             complement(11944..12303)
                     /locus_tag="JQH51_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076047.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12300..12851)
                     /locus_tag="JQH51_001673"
     CDS             complement(12300..12851)
                     /locus_tag="JQH51_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076048.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            complement(12952..13551)
                     /locus_tag="JQH51_001674"
     CDS             complement(12952..13551)
                     /locus_tag="JQH51_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076049.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="JQH51_001675"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="JQH51_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14723..15097)
                     /locus_tag="JQH51_001676"
     CDS             complement(14723..15097)
                     /locus_tag="JQH51_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY6076050.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="JQH51_001677"
     CDS             complement(15101..16249)
                     /gene="wzy"
                     /locus_tag="JQH51_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY6076051.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16269..17138)
                     /locus_tag="JQH51_001678"
     CDS             complement(16269..17138)
                     /locus_tag="JQH51_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HAY6076052.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17167..18057)
                     /locus_tag="JQH51_001679"
     CDS             complement(17167..18057)
                     /locus_tag="JQH51_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY6076053.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="JQH51_001680"
     CDS             complement(18087..19343)
                     /gene="wzx"
                     /locus_tag="JQH51_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY6076054.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="JQH51_001681"
     CDS             complement(19359..19904)
                     /gene="rfbC"
                     /locus_tag="JQH51_001681"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY6076055.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="JQH51_001682"
     CDS             complement(19909..20787)
                     /gene="rfbA"
                     /locus_tag="JQH51_001682"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY6076056.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="JQH51_001683"
     CDS             complement(20846..21745)
                     /gene="rfbD"
                     /locus_tag="JQH51_001683"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY6076057.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="JQH51_001684"
     CDS             complement(21745..22830)
                     /gene="rfbB"
                     /locus_tag="JQH51_001684"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY6076058.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23203..24096)
                     /gene="galF"
                     /locus_tag="JQH51_001685"
     CDS             complement(23203..24096)
                     /gene="galF"
                     /locus_tag="JQH51_001685"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY6076059.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="JQH51_001686"
     CDS             complement(24271..25665)
                     /gene="wcaM"
                     /locus_tag="JQH51_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY6076060.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="JQH51_001687"
     CDS             complement(25676..26896)
                     /gene="wcaL"
                     /locus_tag="JQH51_001687"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY6076061.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="JQH51_001688"
     CDS             complement(26893..28173)
                     /gene="wcaK"
                     /locus_tag="JQH51_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY6076062.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="JQH51_001689"
     CDS             complement(28353..29831)
                     /gene="wzxC"
                     /locus_tag="JQH51_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY6076063.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="JQH51_001690"
     CDS             complement(29833..31227)
                     /gene="wcaJ"
                     /locus_tag="JQH51_001690"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY6076064.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="JQH51_001691"
     CDS             complement(31282..32652)
                     /gene="cpsG"
                     /locus_tag="JQH51_001691"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY6076065.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="JQH51_001692"
     CDS             complement(32845..34281)
                     /gene="cpsB"
                     /locus_tag="JQH51_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY6076066.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="JQH51_001693"
     CDS             complement(34284..35507)
                     /gene="wcaI"
                     /locus_tag="JQH51_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY6076067.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35504..35983)
                     /locus_tag="JQH51_001694"
     CDS             complement(35504..35983)
                     /locus_tag="JQH51_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY6076068.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="JQH51_001695"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35983..36951)
                     /gene="fcl"
                     /locus_tag="JQH51_001695"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY6076069.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="JQH51_001696"
     CDS             complement(36954..38075)
                     /gene="gmd"
                     /locus_tag="JQH51_001696"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY6076070.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="JQH51_001697"
     CDS             complement(38101..38649)
                     /gene="wcaF"
                     /locus_tag="JQH51_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY6076071.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38665..>38786)
                     /locus_tag="JQH51_001698"
     CDS             complement(38665..>38786)
                     /locus_tag="JQH51_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY6076072.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10355 a         9714 c         8728 g         9989 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaattc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttttctatag aaaataccca taaaactaca acgcctataa
    15181 taaaaattcc catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa
    15241 aaagtattaa cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa
    15301 agataagcaa cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta
    15361 tctcaagagg tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc
    15421 caaacaagaa cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa
    15481 cagagtcaga atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc
    15541 tgcccaatat tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata
    15601 acagtgataa tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca
    15661 ctattatgag acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa
    15721 aaaagtagaa ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca
    15781 atgataagtg ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt
    15841 atgaaatata taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct
    15901 cacatgaaaa tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa
    15961 aaaggtatgg ttgcacatca aacagtatat catttatttt attaccagct aataagccga
    16021 caaagaagcc ataaaaagga aataaaagca accatataaa aagactgaca catttttggg
    16081 tgattgaaaa attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata
    16141 ataaatatcg caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa
    16201 tcagttcaat acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt
    16261 gatgtttttt aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt
    16321 ttccgcctta acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc
    16381 aattgattat tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt
    16441 cgataccact tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt
    16501 gctcccactt ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac
    16561 cacgaataat ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct
    16621 tgtgcagcat ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt
    16681 ataacatatg tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac
    16741 tgagccttat caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa
    16801 taacaattcg cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac
    16861 caaatatcgt cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta
    16921 attaatctat aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt
    16981 ataattgagg gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat
    17041 aaatcatctg atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat
    17101 tgttcctcaa tccagcattc gccattatac gtagccataa gtacagctac tcttttttga
    17161 tcagaactat gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc
    17221 caaaatatgt tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt
    17281 atgaattaaa gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct
    17341 aagcaaaaga aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat
    17401 gttaatccca cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg
    17461 aatgatatga actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata
    17521 atcaataaaa agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat
    17581 ggttccggat gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat
    17641 tttctctcga agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct
    17701 atcaataaaa actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt
    17761 tttgataaac tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc
    17821 tttttcaaga gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc
    17881 aagacaaatt aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt
    17941 cctattatcg actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt
    18001 cagattttta agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat
    18061 ccaagtgact cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa
    18121 agccattgta ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc
    18181 agtaacccat atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca
    18241 caatgaatca aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc
    18301 attgccagcg cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta
    18361 atttgataat tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg
    18421 attatcaccg gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta
    18481 aattctttaa agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca
    18541 ggtattattc ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct
    18601 gcccccataa tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata
    18661 ttacttactg tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa
    18721 tgaacccccg aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata
    18781 actcttgcaa atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat
    18841 atggccggaa ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca
    18901 aatttttcat ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg
    18961 caaatagcca acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt
    19021 agataattaa caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat
    19081 agcaccaaaa aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga
    19141 tgaattgcaa tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac
    19201 ccaacaagtg caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg
    19261 agaaatccta gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag
    19321 acactatttt ttattatgct cattattaca tttctttatc atataaaata atcagcatct
    19381 acaaattttt gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct
    19441 atatttatac ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta
    19501 tttgttttgt agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct
    19561 tccggtatcc ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca
    19621 aatgttggtg atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag
    19681 cgtaccagtt ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc
    19741 ttagttgatt tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg
    19801 aaaactttct gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt
    19861 tcaaaaatta gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt
    19921 aatcattttc agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt
    19981 cacttgctca gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt
    20041 cagcccctga cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg
    20101 tgtccccgtg tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg
    20161 gtccatataa atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag
    20221 gtttttcgcc atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt
    20281 actttttggt tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc
    20341 caccacaccg tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc
    20401 tctgttaaca gcagtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc
    20461 aagtaccagc gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc
    20521 cagaccatcc ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc
    20581 accaagcaat tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg
    20641 aatacccgcc aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg
    20701 tagcagttgt ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc
    20761 cagaataata cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc
    20821 atcatgagcg agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca
    20881 tacgtttcac gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt
    20941 cggtattgag gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca
    21001 cggcattaag tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca
    21061 gagcggcata atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa
    21121 cttctggttt atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag
    21181 cccccgttgg tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca
    21241 ggcgcaacat cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga
    21301 aaataagatg ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac
    21361 cgtagacatt tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg
    21421 ggaaaacata atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg
    21481 caatcgcttc gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt
    21541 tgtctactgc agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg
    21601 tttcagccac accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga
    21661 gcgcaatcaa gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg
    21721 ttttgccgaa aagcaggata ttcattactg acggccctca tagttttgtt caatccacga
    21781 ctgataagta ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac
    21841 cgttttacga atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat
    21901 tttatcggca tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat
    21961 ttgctcacga taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag
    22021 cactacgtcg atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt
    22081 accttcggtt acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg
    22141 aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac
    22201 caatggaata agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac
    22261 aatggttggt aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt
    22321 agacgcagaa tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc
    22381 ttcgttatta tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat
    22441 atgatgaaag cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc
    22501 cgcttctaaa agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt
    22561 tattgaacgg tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg
    22621 tgcgaaaata cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg
    22681 ttcagaatca gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac
    22741 attaacaaca ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa
    22801 ccctgcgcca ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc
    22861 tgtgaaatag actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg
    22921 gcttaacagc taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg
    22981 cgccaaatcc gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa
    23041 aatcctggaa aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata
    23101 aaaacggcaa cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg
    23161 ttataatttt cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt
    23221 ctcaatacct ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa
    23281 cgcctgcata tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc
    23341 atcaacggat tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca
    23401 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc
    23461 tacggccatg atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac
    23521 aatgcggctg actttaccct cacggtccag cggctcttta gtctggatga cggagtattc
    23581 agagaggtca cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg
    23641 tgcaatcatg gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac
    23701 gtctggcagt accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga
    23761 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca
    23821 gatggactgt acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa
    23881 ctcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag
    23941 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
    24001 gacgattggt agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag
    24061 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
    24121 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
    24181 gaattacttg tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat
    24241 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
    24301 aatcttttcc acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc
    24361 tctttcattc accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa
    24421 gacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga
    24481 ttcctgcatc acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag
    24541 cgaggcacgc ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga
    24601 gatttggatg ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac
    24661 tcggaaattt tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
    24721 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
    24781 gactgtcgcg ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt
    24841 gatattacga ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga
    24901 tcccgtgata ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc
    24961 ataagtgctt cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt
    25021 gatacgctcg atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca
    25081 ttcaatggcg tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat
    25141 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc
    25201 gatggtcagg ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg
    25261 gccaaagccg ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt
    25321 aatgttatgc ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa
    25381 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat
    25441 gaagatgccg gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc
    25501 ttcgctgaag gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt
    25561 aacgctttgt cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga
    25621 gcttgccgtc aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata
    25681 aagcctgcag caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga
    25741 ctttttcgca cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg
    25801 ccgccagacg ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat
    25861 cggcctccac cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg
    25921 ccatcgcttc cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg
    25981 atggcaacag gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa
    26041 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc
    26101 gttcccacag gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
    26161 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata
    26221 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca
    26281 cgcccatacg cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg
    26341 cccacagatc gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg
    26401 gagtgtagtg gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa
    26461 ttttgccgcg aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa
    26521 aatgagcgat gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca
    26581 aaatcaggtt ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt
    26641 tacgatgaat gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg
    26701 caggttcgtc ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt
    26761 gggtgttctc tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa
    26821 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga
    26881 agaagccgac cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat
    26941 tcccgtctga cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg
    27001 gcctaaggta tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat
    27061 cgccatctct ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat
    27121 ggcaatcgcc ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac
    27181 ggtgagttca caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac
    27241 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc
    27301 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
    27361 atcgagaatg cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc
    27421 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc
    27481 ggcaacgtcc agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc
    27541 gaccagccac gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt
    27601 catcaaatca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata
    27661 gttcgccagt tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa
    27721 cagcggcttt ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag
    27781 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
    27841 gaagtcggtg aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg
    27901 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac
    27961 gcccgccgcg ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg
    28021 gttgagcagc caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg
    28081 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
    28141 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
    28201 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta
    28261 tcaggcctac aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc
    28321 cggcaaccgt tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt
    28381 ttttcgctgc gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca
    28441 atcatcacca caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc
    28501 gccagttgcc ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag
    28561 agataaaacg gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt
    28621 ttaatcatca cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc
    28681 gtgacgccga ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat
    28741 gttttgaata cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa
    28801 ccaatcgggt tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga
    28861 ataatgctgt tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc
    28921 agccccagca gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta
    28981 acacgcagct tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg
    29041 atgattgggt tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc
    29101 cctgccacgc ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga
    29161 tagttgatga tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac
    29221 gccagcgaga agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt
    29281 gttctcaccg cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa
    29341 tgggcgctaa ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg
    29401 attttgttga actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc
    29461 accacaaacg ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg
    29521 tcgccgatga catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg
    29581 ttcagccagt acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag
    29641 ttagcgatac cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc
    29701 agcaggccga actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg
    29761 aggccgatga tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca
    29821 cgtaagctca tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac
    29881 gattttgata tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg
    29941 tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc
    30001 ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg
    30061 ttcgttatgc gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac
    30121 attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt
    30181 gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat
    30241 ggaacggaac ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa
    30301 aataaccggc cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga
    30361 gatcagcagc agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt
    30421 aacccccgaa agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag
    30481 acgtgaatgg agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt
    30541 atccgccagt tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat
    30601 atagacgtta tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc
    30661 ccagtcgtta gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc
    30721 taaccacggc tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc
    30781 tacggcgacc atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat
    30841 acatgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt
    30901 cagttgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa
    30961 ggtccagttt tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg
    31021 ataaaaatcg gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa
    31081 caggtgcatg tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa
    31141 catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc
    31201 tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt
    31261 ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc
    31321 ttccatcagc ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt
    31381 ggaggtgcgc aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc
    31441 caccgccagc gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg
    31501 cgccagtttg ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag
    31561 ttcgcccagc gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat
    31621 gccgctgtcg cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta
    31681 gatggcgtct tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac
    31741 cggcgtgccg cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg
    31801 gatgatcttc gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta
    31861 gtagccctcg ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc
    31921 aaaggcaatg cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca
    31981 ttccggcagc agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac
    32041 tttgattaat tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac
    32101 cggacccgct gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac
    32161 attgatataa ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg
    32221 accgcgtttg gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc
    32281 gcgcagtccg gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt
    32341 gtaatccatc ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa
    32401 cgtggcgaaa tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc
    32461 atcctgtaaa ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc
    32521 gcctaacaca atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc
    32581 gatatcttca ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt
    32641 taattttttc atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa
    32701 ttttattttt gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc
    32761 gcgtcttatc aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc
    32821 gacaaacagc gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac
    32881 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    32941 cgggttttcc aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag
    33001 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc
    33061 cgcgcggtga tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt
    33121 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc
    33181 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    33241 atcctgtacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    33301 atctttcacc ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt
    33361 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc
    33421 ccataatgaa gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc
    33481 atctgccgta ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    33541 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca
    33601 ggcatcgagg atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    33661 caggaacatg ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc
    33721 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac
    33781 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    33841 aaaggtcacc agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa
    33901 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    33961 cgggctatga cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc
    34021 tgccggttcg agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac
    34081 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    34141 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    34201 gtaaagtacg cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg
    34261 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    34321 ttctctttat cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc
    34381 aggagcgcct gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    34441 gcaatacccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    34501 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    34561 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg
    34621 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    34681 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    34741 aggagatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    34801 taaagaatga tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg
    34861 gcgtctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta
    34921 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg
    34981 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg
    35041 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    35101 tgcagcacgg tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac
    35161 agcgttggca ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga
    35221 aagaaactgc tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt
    35281 tttggcacgt acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc
    35341 caggcggaat agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc
    35401 cgcacctcat gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg
    35461 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    35521 cggcacgctt ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga
    35581 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct
    35641 cttcttccgc tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    35701 cggagaagtt atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg
    35761 gcagacgcag ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt
    35821 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    35881 tgccaagcag aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg
    35941 tggagcgcac tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg
    36001 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    36061 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    36121 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    36181 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    36241 ttgggtattc tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc
    36301 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    36361 cggcgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga
    36421 gttactcggg tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta
    36481 atcgcgtcca tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat
    36541 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    36601 ctgttttgcc agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc
    36661 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    36721 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    36781 gtcaatgcgt tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc
    36841 atcgcgggta cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc
    36901 agaaccgacc atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    36961 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    37021 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    37081 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg
    37141 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    37201 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    37261 atgcccagct gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc
    37321 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    37381 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    37441 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    37501 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    37561 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    37621 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    37681 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    37741 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    37801 acgtggctca ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    37861 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    37921 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    37981 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    38041 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    38101 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    38161 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    38221 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    38281 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    38341 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    38401 gtcatcgcca acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    38461 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    38521 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    38581 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    38641 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt
    38701 cgtacgttgg cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg
    38761 ccgttggact tttttggcgt cagcac
//
LOCUS       DADLEE010000032        38228 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000032 DADLEE010000000
VERSION     DADLEE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38228
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.032"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..74)
                     /locus_tag="JQH51_001699"
     CDS             complement(<1..74)
                     /locus_tag="JQH51_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HAY6076073.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="JQH51_001700"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="JQH51_001700"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY6076074.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="JQH51_001701"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="JQH51_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY6076075.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="JQH51_001702"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="JQH51_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY6076076.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="JQH51_001703"
     CDS             2704..2940
                     /locus_tag="JQH51_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY6076077.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="JQH51_001704"
     CDS             2943..3800
                     /locus_tag="JQH51_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6076078.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="JQH51_001705"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="JQH51_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="JQH51_001706"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="JQH51_001706"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY6076079.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="JQH51_001707"
     CDS             complement(6415..7311)
                     /locus_tag="JQH51_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY6076080.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="JQH51_001708"
     CDS             complement(7360..7599)
                     /locus_tag="JQH51_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY6076081.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /locus_tag="JQH51_001709"
     CDS             7702..8301
                     /locus_tag="JQH51_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6076082.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="JQH51_001710"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="JQH51_001710"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY6076083.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="JQH51_001711"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="JQH51_001711"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY6076084.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="JQH51_001712"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="JQH51_001712"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY6076085.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="JQH51_001713"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="JQH51_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /gene="tnpB"
                     /locus_tag="JQH51_001714"
     CDS             complement(15268..16476)
                     /gene="tnpB"
                     /locus_tag="JQH51_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY6076086.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16484..16984
                     /gene="tnpA"
                     /locus_tag="JQH51_001715"
     CDS             16484..16984
                     /gene="tnpA"
                     /locus_tag="JQH51_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY6076087.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="JQH51_001716"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="JQH51_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY6076088.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /locus_tag="JQH51_001717"
     CDS             17705..18532
                     /locus_tag="JQH51_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6076089.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="JQH51_001718"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="JQH51_001718"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY6076090.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="JQH51_001719"
     CDS             complement(19387..20556)
                     /locus_tag="JQH51_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076091.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="JQH51_001720"
     CDS             complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="JQH51_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY6076092.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="JQH51_001721"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="JQH51_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY6076093.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /locus_tag="JQH51_001722"
     CDS             complement(22132..23064)
                     /locus_tag="JQH51_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6076094.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /locus_tag="JQH51_001723"
     CDS             23177..24388
                     /locus_tag="JQH51_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY6076095.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="JQH51_001724"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="JQH51_001724"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY6076096.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="JQH51_001725"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="JQH51_001725"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY6076097.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="JQH51_001726"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="JQH51_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY6076098.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="JQH51_001727"
     CDS             complement(28137..29393)
                     /locus_tag="JQH51_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076099.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="JQH51_001728"
     tRNA            29701..29777
                     /locus_tag="JQH51_001728"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="JQH51_001729"
     tRNA            29782..29858
                     /locus_tag="JQH51_001729"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="JQH51_001730"
     CDS             29966..30271
                     /locus_tag="JQH51_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY6076100.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="JQH51_001731"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="JQH51_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /locus_tag="JQH51_001732"
     CDS             complement(31831..32643)
                     /locus_tag="JQH51_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076101.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /locus_tag="JQH51_001733"
     CDS             complement(32647..33432)
                     /locus_tag="JQH51_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY6076102.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="JQH51_001734"
     CDS             complement(33429..34097)
                     /locus_tag="JQH51_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY6076103.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="JQH51_001735"
     CDS             complement(34161..34799)
                     /locus_tag="JQH51_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY6076104.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36891)
                     /locus_tag="JQH51_001736"
                     /pseudo
     CDS             complement(34812..36891)
                     /locus_tag="JQH51_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36912..37538)
                     /locus_tag="JQH51_001737"
     CDS             complement(36912..37538)
                     /locus_tag="JQH51_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY6076105.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(37994..38203)
                     /gene="fumD"
                     /locus_tag="JQH51_001738"
     CDS             complement(37994..38203)
                     /gene="fumD"
                     /locus_tag="JQH51_001738"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY6076106.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9203 a         9986 c         9362 g         9677 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgcgtac
    36181 ttggcacgac atggttgtta ttcgcaccaa taagttcagt catcatgtaa tcattgagac
    36241 gcttcatctc ctgacgatcg gcaatgttga cccctttcgt cccttcaacc gcaatcgctt
    36301 tcaggttttt cgaacccatt attgcgccag ttcccgcacc gccgctgtgg ttacggctat
    36361 tcaacatgcc agagagagga ataaggtttt ccccagcctg accaatagcc gccacacagg
    36421 tttccgggct ggtcaatcga caaatttctt ccgtcgttgc gcgcgtccct tttccccata
    36481 agaaatcggc tttttccagg ctaactttgt catctttaat cttcagccat accggtgatt
    36541 tcgccttccc ttcgataata atgacgtcgt atccagcgaa tttcatttgc gctgcaaaaa
    36601 agccacccat atgggcatcg cgactaaatt tcctttggta aaagtagaaa gtgaggtgat
    36661 atttacgcga gaactacagg gggcaccaga tccaggtaat gggccggtag caaagactaa
    36721 tttattcgct tcatcgaaag gtttcgtgcc tagcggtact tcgtcataca taattttgta
    36781 gccgaagccc atgccaccga caaaactttt aaacttactg gaatcttcga gggtaatatt
    36841 tcctgtcgtg agattgactc ttaatatatt acctgtccaa ccgttagcca tgattttttc
    36901 ctttgcaaga ttcacacagt aatatctttc cactcgataa tttttaacgc ccctgttggg
    36961 caggcgtttg cgcattcacc gcataacaca catttcgagg attttttact ttcggtattt
    37021 acggtggcca tcatccacgg gcatgccgta gtacaggcgc tacagccaat acaacgttta
    37081 tgatcgacgg taatacagcc ttctttctgc tgccaggtaa tcgcaccaat cgggcagacg
    37141 ttcatgcatt gcggctcttt gcattgacgg caggtgtccg cggtatagtt gagatcgcca
    37201 tacaggccgc cgccagagcc aaccccgttg tcgccaaaga aataattgcg atggattttg
    37261 atacgggaga agaatgtccc tactgagcca tcgttgaagt tggtacaaga gatttcacag
    37321 cggtggcaac ccgtgcatcg cgctcgctgg gtcaccagca cccctttggg tgtatttatc
    37381 aaccctactg tgccgctatc aatatcttcc tgtttgcaac ccaaaagtga cagtaacgca
    37441 ggggcaattg ttagaccagc aaggcctttt cctgatatac gcaagaattc taatcgcgtt
    37501 aagccaatat ctaatagtgg acgatcaacc gggttcattt atttcgcatc ctcttcgcaa
    37561 acacagtgct gacaattcag cagcctgata ttaagcattc aactaatacg ctgctgactc
    37621 tgtccccaac accagatatt ttccctaacc cgatagtgta tgagaaaaga tttaacgccg
    37681 ataaactaat cctttatggt taggagaaaa aataatgtga ttattctgag cccttaacat
    37741 tgatcgttat caattaaaat tacaaagcaa gaaattataa gcgaactgat atttattatc
    37801 atttgaaata aatttaactt aagcgacaat catttgtgac gttcattgca gaaaataaaa
    37861 ataacattat cgctataaat taaaatacat accgaaattc gtataatttc agcagttgtt
    37921 atttagaatg attaactgga ccgcaactga agcaagaata tcaggatgaa gcttaccgcc
    37981 tcatcctgaa tggtcatttt accagtgcgt taatgacggt ttccatcgcc tgcttttcca
    38041 gctcactgcg ctgaatgcgt ttattggcta atgctgtacg taatacgcct gcgataacaa
    38101 tatgctttgg ttcctgcccc agatcgcgca tttccagcac aactttaccg acaactctgc
    38161 acatttctcg gtacagttca tcctcttttg tccgatttcc cattctttat acctatttat
    38221 catgccaa
//
LOCUS       DADLEE010000033        37628 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000033 DADLEE010000000
VERSION     DADLEE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.033"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..176)
                     /locus_tag="JQH51_001739"
     CDS             complement(<1..176)
                     /locus_tag="JQH51_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY6076107.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="JQH51_001740"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="JQH51_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY6076108.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="JQH51_001741"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="JQH51_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY6076109.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="JQH51_001742"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="JQH51_001742"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY6076110.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="JQH51_001743"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="JQH51_001743"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY6076111.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="JQH51_001744"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="JQH51_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY6076112.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="JQH51_001745"
     CDS             complement(5180..5422)
                     /locus_tag="JQH51_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY6076113.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="JQH51_001746"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="JQH51_001746"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY6076114.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="JQH51_001747"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="JQH51_001747"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY6076115.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="JQH51_001748"
     CDS             complement(8339..9313)
                     /locus_tag="JQH51_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6076116.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="JQH51_001749"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="JQH51_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY6076117.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /locus_tag="JQH51_001750"
     CDS             11710..12753
                     /locus_tag="JQH51_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY6076118.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="JQH51_001751"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="JQH51_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY6076119.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="JQH51_001752"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="JQH51_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY6076120.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="JQH51_001753"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="JQH51_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY6076121.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="JQH51_001754"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="JQH51_001754"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY6076122.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /locus_tag="JQH51_001755"
     CDS             16539..20339
                     /locus_tag="JQH51_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY6076123.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="JQH51_001756"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="JQH51_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY6076124.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="JQH51_001757"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="JQH51_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY6076125.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="JQH51_001758"
     CDS             23180..23383
                     /locus_tag="JQH51_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY6076126.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="JQH51_001759"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="JQH51_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY6076127.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="JQH51_001760"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="JQH51_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY6076128.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="JQH51_001761"
     CDS             26388..26660
                     /locus_tag="JQH51_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076129.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="JQH51_001762"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="JQH51_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY6076130.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="JQH51_001763"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="JQH51_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY6076131.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="JQH51_001764"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="JQH51_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY6076132.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="JQH51_001765"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="JQH51_001765"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY6076133.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="JQH51_001766"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="JQH51_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="JQH51_001767"
     CDS             complement(31907..32305)
                     /locus_tag="JQH51_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY6076134.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /locus_tag="JQH51_001768"
     CDS             32499..33626
                     /locus_tag="JQH51_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY6076135.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="JQH51_001769"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="JQH51_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY6076136.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="JQH51_001770"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="JQH51_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY6076137.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="JQH51_001771"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="JQH51_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY6076138.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQCVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="JQH51_001772"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="JQH51_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY6076139.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQH51_001773"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQH51_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY6076140.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9414 c         9900 g         9200 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggct cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagtgcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DADLEE010000034        36500 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000034 DADLEE010000000
VERSION     DADLEE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36500
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.034"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(156..1391)
                     /locus_tag="JQH51_001774"
     CDS             complement(156..1391)
                     /locus_tag="JQH51_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY6076141.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JQH51_001775"
     tRNA            complement(1538..1614)
                     /locus_tag="JQH51_001775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="JQH51_001776"
     tRNA            complement(1657..1743)
                     /locus_tag="JQH51_001776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="JQH51_001777"
     tRNA            complement(1764..1839)
                     /locus_tag="JQH51_001777"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="JQH51_001778"
     tRNA            complement(1898..1974)
                     /locus_tag="JQH51_001778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="JQH51_001779"
     CDS             complement(2077..3462)
                     /locus_tag="JQH51_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6076142.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="JQH51_001780"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="JQH51_001780"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY6076143.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="JQH51_001781"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="JQH51_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY6076144.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="JQH51_001782"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="JQH51_001782"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY6076145.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="JQH51_001783"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="JQH51_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY6076146.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /locus_tag="JQH51_001784"
     CDS             complement(8073..9203)
                     /locus_tag="JQH51_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY6076147.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="JQH51_001785"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="JQH51_001785"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY6076148.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="JQH51_001786"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="JQH51_001786"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY6076149.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="JQH51_001787"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="JQH51_001787"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY6076150.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="JQH51_001788"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="JQH51_001788"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY6076151.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="JQH51_001789"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="JQH51_001789"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY6076152.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="JQH51_001790"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="JQH51_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY6076153.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="JQH51_001791"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="JQH51_001791"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY6076154.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="JQH51_001792"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="JQH51_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY6076155.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="JQH51_001793"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="JQH51_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY6076156.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="JQH51_001794"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="JQH51_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY6076157.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="JQH51_001795"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="JQH51_001795"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY6076158.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="JQH51_001796"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="JQH51_001796"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY6076159.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="JQH51_001797"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="JQH51_001797"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY6076160.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="JQH51_001798"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="JQH51_001798"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY6076161.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="JQH51_001799"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="JQH51_001799"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY6076162.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="JQH51_001800"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="JQH51_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY6076163.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="JQH51_001801"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="JQH51_001801"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY6076164.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="JQH51_001802"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="JQH51_001802"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY6076165.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="JQH51_001803"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="JQH51_001803"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY6076166.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="JQH51_001804"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="JQH51_001804"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY6076167.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="JQH51_001805"
     CDS             complement(31267..32913)
                     /locus_tag="JQH51_001805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY6076168.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="JQH51_001806"
     CDS             complement(32916..32966)
                     /locus_tag="JQH51_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076169.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="JQH51_001807"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="JQH51_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY6076170.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="JQH51_001808"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="JQH51_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="JQH51_001809"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="JQH51_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY6076171.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="JQH51_001810"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="JQH51_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY6076172.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="JQH51_001811"
     tRNA            complement(36398..36473)
                     /locus_tag="JQH51_001811"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
BASE COUNT         8920 a         9981 c         9200 g         8399 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tattttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact
//
LOCUS       DADLEE010000035        35789 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000035 DADLEE010000000
VERSION     DADLEE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35789
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.035"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..246
                     /locus_tag="JQH51_001812"
     CDS             <1..246
                     /locus_tag="JQH51_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076173.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JQH51_001813"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JQH51_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY6076174.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JQH51_001814"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JQH51_001814"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY6076175.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="JQH51_001815"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="JQH51_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY6076176.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /locus_tag="JQH51_001816"
     CDS             complement(3257..3541)
                     /locus_tag="JQH51_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076177.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="JQH51_001817"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="JQH51_001817"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY6076178.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="JQH51_001818"
     CDS             complement(4442..5650)
                     /locus_tag="JQH51_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076179.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /locus_tag="JQH51_001819"
     CDS             5734..6270
                     /locus_tag="JQH51_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6076180.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="JQH51_001820"
     CDS             complement(6445..8130)
                     /locus_tag="JQH51_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY6076181.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="JQH51_001821"
     CDS             8400..8777
                     /locus_tag="JQH51_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076182.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="JQH51_001822"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="JQH51_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY6076183.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="JQH51_001823"
     CDS             9224..9511
                     /locus_tag="JQH51_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY6076184.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="JQH51_001824"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="JQH51_001824"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY6076185.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="JQH51_001825"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="JQH51_001825"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY6076186.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="JQH51_001826"
     CDS             11268..11744
                     /locus_tag="JQH51_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY6076187.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="JQH51_001827"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="JQH51_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY6076188.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="JQH51_001828"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="JQH51_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY6076189.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="JQH51_001829"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="JQH51_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY6076190.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="JQH51_001830"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="JQH51_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY6076191.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="JQH51_001831"
     CDS             16301..16789
                     /locus_tag="JQH51_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY6076192.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="JQH51_001832"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="JQH51_001832"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY6076193.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="JQH51_001833"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="JQH51_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6076194.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="JQH51_001834"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="JQH51_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY6076195.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="JQH51_001835"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="JQH51_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY6076196.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="JQH51_001836"
     CDS             complement(20546..21277)
                     /gene="artJ"
                     /locus_tag="JQH51_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6076197.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="JQH51_001837"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="JQH51_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY6076198.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="JQH51_001838"
     CDS             complement(22241..22756)
                     /locus_tag="JQH51_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6076199.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..25174
                     /locus_tag="JQH51_001839"
     CDS             23405..25174
                     /locus_tag="JQH51_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY6076200.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25385..25708
                     /locus_tag="JQH51_001840"
     CDS             25385..25708
                     /locus_tag="JQH51_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY6076201.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25705..26535
                     /locus_tag="JQH51_001841"
     CDS             25705..26535
                     /locus_tag="JQH51_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY6076202.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26532..27545)
                     /locus_tag="JQH51_001842"
     CDS             complement(26532..27545)
                     /locus_tag="JQH51_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY6076203.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27644..29074)
                     /locus_tag="JQH51_001843"
     CDS             complement(27644..29074)
                     /locus_tag="JQH51_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6076204.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="JQH51_001844"
     CDS             complement(29085..30086)
                     /gene="ltaE"
                     /locus_tag="JQH51_001844"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY6076205.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30123..31871)
                     /gene="poxB"
                     /locus_tag="JQH51_001845"
     CDS             complement(30123..31871)
                     /gene="poxB"
                     /locus_tag="JQH51_001845"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY6076206.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            complement(32004..32972)
                     /gene="hcr"
                     /locus_tag="JQH51_001846"
     CDS             complement(32004..32972)
                     /gene="hcr"
                     /locus_tag="JQH51_001846"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY6076207.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32984..34636)
                     /gene="hcp"
                     /locus_tag="JQH51_001847"
                     /gene_synonym="priS"
     CDS             complement(32984..34636)
                     /gene="hcp"
                     /locus_tag="JQH51_001847"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY6076208.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34780..35679)
                     /gene="lysO"
                     /locus_tag="JQH51_001848"
     CDS             complement(34780..35679)
                     /gene="lysO"
                     /locus_tag="JQH51_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY6076209.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8918 a         8848 c         9199 g         8824 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccatgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgtttagat ctgttaaaat
    24661 taataacgta aatactcatt ctttaaaatt tattaactgc aattttttca atgttgatat
    24721 gataagagtt aatctcagca aatgtctttt tcatgaatgt agtatgcatg gtgtcaagat
    24781 aaaaccctgg ttgcctgtaa aatggacaaa agaattaata agtgattatt tgtatggctg
    24841 tttactatct ctgtactcta tctgtgcaag ggatatatat aatatgaacg ccgggaataa
    24901 cgtgaaggtt gctgcggatg ctttccttga aattattttc tctcttaaaa ataaatattg
    24961 tattaaacta ttaagcgctc aggatcgggc gtttatatac gagtttgcaa gaatgatctt
    25021 cgcttatatt aatgacaaaa gcattgagat cttattactt tcttgttttg cggcggctga
    25081 ccaaaaagca atccagcggt atagacctca gtcacaagat ggggaagact ttaggagtca
    25141 tttacaatat aagttgcctc tttccgctca ttaatgttgt tggtgcaaaa taataagaca
    25201 ggtaagcggt accctataat gaaaccaata tcgggttatg caacggataa tcgtaaagac
    25261 ttaagtgggt gtttttgaca caatgagatg atttcctaaa ctgatcaaaa ccttcttccc
    25321 aacatggcat atcagtccga taatgcttta gagatcgaag gcttaacaaa caaggagatc
    25381 gatcatgcaa ttttctacaa ccccaactct ggaaggcctg accatcgttg aatattgcgg
    25441 tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct ttgccggtat
    25501 ccgcgatatc gttggcggac gttccggtgc gtatgaaaaa gaactgcgta aagcacggga
    25561 gatcgccttt gaggaattag gctcccaggc gcgggcgctg ggggccgatg ccgtagttgg
    25621 tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg ttagcgtcag
    25681 cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctggtcgc tgccgctctg
    25741 ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata tcagcttgat
    25801 acccgacacc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat ccactacacc
    25861 gcagatgatt ttgatagctc gctggcgaca ctgactgata agcaggtcag ctcgcattat
    25921 ctgatccctg cggtgccacc gcgatacaac ggtaaaccgc gcatctggca actggtgccg
    25981 gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac gcgccttaac
    26041 gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcatc cggagtgaaa
    26101 tattttgccc cgtttgaaca ggcacagatt caggcgctta ttccgctggc gaaagatatt
    26161 attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat cgcaccgcag
    26221 cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca ggggattggt
    26281 gcctggccgg atgcgcagcg ggtgaacttt taccttgccg ggcgcgcgcc gcacacaccg
    26341 gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt aaaacctgat
    26401 atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt ccgcccgacg
    26461 ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt gctggagaaa
    26521 tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc agttttctcg
    26581 atgccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc acgcgtaata
    26641 tccagcgtaa agtcaaaatt caacttggag gcgccgtagt gggtcagcgg cggctctttt
    26701 gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat cgggtaaggg
    26761 acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac gatgctgcgc
    26821 agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag cttatcgcag
    26881 gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt catatcgacc
    26941 agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat atgcgccaga
    27001 cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag aatagtaaag
    27061 cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc tgctttgctg
    27121 cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatt gcgatggtgg
    27181 tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac accccaggcg
    27241 acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa agcctgttgt
    27301 gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc cgcgagcatc
    27361 actttagctt gtgatgaaac cagctcggtc agatccgtcg gaacaaactc tgcgcccatt
    27421 ttttccagca atttacccat tgcttcgttg cgaccgctcg cacgcacgct gatgcctttc
    27481 tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt aaccagtacc
    27541 ttcatatcaa tccactgttg ttgagaaaat gacgtgcgca ttcttccgtg atttccccca
    27601 tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct gtgctttgtt
    27661 ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc gcggggatca
    27721 tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg cggacatcga
    27781 tagtacgata gtcaccttta tcttccaggg taaaacacag tcgtcccagc cccggcgctt
    27841 tcatgccaaa taacaacgta agttgctttt tcggttcaac gacaatcact ttccagctat
    27901 ccaccgcatc gccggtctgt aaatattcgc gctccgggcg gcctttcgcc agattatgac
    27961 cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag aaataacgct
    28021 ctttaccgcc gatttggttc accaccagcc ataaagccgc aaggctggcg gacgttttaa
    28081 cggtaaaccc cgcctgtttg gcgaaataac cgtactccgg tcgccagcgg gcaaaggcct
    28141 gagcgtcgta gccccagtcg ctggagttga ccagtttttc ttcctctttc aacgtgctac
    28201 gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc gcggtatcat
    28261 ctgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg ggcggtacgg
    28321 aagtaatcac attgagaaac cacaccgaaa tccagcgggt ggggaggggg atggggatca
    28381 accagcggcg cttaccgctc accgccataa aatgttcaaa ctgttgctga taactgagca
    28441 cctctggtcc ggcggcttcg aagatgcggt gttcgttggc tggatggtct aacagcgcca
    28501 ccagatagtg cagcaagttt tccagcgcga tgggcgtggt gcgtgaacgt acccagcgtg
    28561 gcggcgttaa cactggcagg ttgtagacca tatcgcgcat gacttcgaac gccgctgaac
    28621 ctgcgccaac gataattccg gcccgaagtt cggtcactgg tacattcgct tcacgaagaa
    28681 tgtccgccgt agcctgacga gctcgcagat gatccgactg ctcatgtggc ggggcctgca
    28741 acgaactgag aaagattaat tgcttaactg gtacttcacg tagcgcatcg cggacgttga
    28801 gagccagctg gcgctcctga gcgataaaat cgccgccttc gcccatgctg tgcaccagaa
    28861 aatagacggt atcgatatcc tgcaacaggg ccggaaggtt atccggccag ctgagatcga
    28921 ctttatggca actgacgttt gccagttgca gctttgcaag cctgtcgaca tgacgtgccg
    28981 ccgctaggat ctgatgccct tgctggctga gtgtgcgcac cagatgctga ccaatgtagc
    29041 cactggcacc gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat
    29101 gcgcgccagt gggcggcgac ttccgccagt tgttcgcgcg agacgtcaag atgcgtcacc
    29161 aggcggacaa tcggcgaggc gttaatcagc acgtttctcg ctttcatgta ttcgcctaac
    29221 gcggcagcat tttcttcccc gacgcgaaca aacagcatat tagtgtcctg acgcatcaca
    29281 tccgcgcctg cttcacgcag ttgctccgcc atccaggcgg cgttgtcgtg atcttcctgc
    29341 aaccgtgcga cgttattttc cagggcatac atcccggcgg cagccagaat cccggactga
    29401 cgcatcccgc caccggccat tttccgccag cggatggcac gtttaatgta atcacgatta
    29461 ccgacgagta atgaaccgac tggcgtccca agacctttcg acaggcaaat ggtgaacgaa
    29521 tcacaatatt gcgtgatctc tttcagttcg cagccgtaag ccaccacggc attaaagatg
    29581 cgcgcaccgt caacatgcag cgccagattg cgctcgcggg taaattccca tgcttctttc
    29641 aggtattcac gcggcagcac tttgccgttg tgggtgtttt ccagactgag taatttggtg
    29701 cgggcgaaat ggatatcgtc gggtttgatt ttcatcgcca ctttatccag cggtagcgtg
    29761 ccgtcggcag ccgcgtcgat gggttgcggc tgaatactgc ccagcaccgc tgcgccaccg
    29821 gcttcaaaca gatagttatg cgcggcctga ccgacaatat actcttcgcc acgttcgcag
    29881 tgactgagca gagcgaccag gttggcctga gtgccggtag gcagaaaaat ggcggcttct
    29941 ttaccggaaa gctctgctgc atagtcctgc agagcattaa cggtagggtc gtctccgtaa
    30001 acgtcgtccc caaccggggc ggccatcatc gcttcgagca tggcgcggct cggtcgggta
    30061 acggtatcac tgcgtaaatc aatcatggca tgtccttatt atgacgggaa atgccaccct
    30121 ttttacctta gccagttcgt tttcgccagt tcgatcactt catcgccgcg tccgctgata
    30181 attgcgcgca acatatacag gctaaaacct ttggcctgtt cgagtttgat ctgcggtgga
    30241 atggctaatt cttctttggc gaccaccaca tccaccaaca ccggaccgtc gatagagaag
    30301 gcgcgttgca gggcttcatc aacttcagag gctttttcta cacggatacc cgtaatgccg
    30361 cacgcttcgg caatgcgggc aaagttggtg tcgtgcagct cagtaccgtc tgtcaggtaa
    30421 cctccggcct tcatctccat cgccacaaag cccagcacgc tgttattaaa gacgataatt
    30481 ttcaccggca gtttcatctg cactactgag aggaaatcgc ccatcaacat gctaaaaccg
    30541 ccatcgccgc acatggcgac cacctgacgt tccggctcgg ttgcctgcgc acccaacgcc
    30601 tgcggcatgg cgttagccat cgaaccgtgg ttaaacgaac ctaacaggcg acgcttgccg
    30661 ttcattttca gataacgtgc cgcccatacc gttggcgtgc cgacgtcaca ggtaaagatg
    30721 gcgtcatcgg cggcaaaatg actaatttgc tgcgccagat attgcgggtg aatggctttc
    30781 tcgctcggtt tagctaaatc gtccagccct ttgcgggcat cgcggtaatc ttccagccct
    30841 ttgcgggcat cgcggtaatc ttccagcgct ttatccagaa acttgcgatc ggttttttct
    30901 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    30961 accttgctat gcgcgccgat gctggctgga ttaatatcaa tctgaataat tttggcatcg
    31021 gtcgggtaga acgcacggta ggggaactga gtaccgagta gcactaacgt gtcggcgttc
    31081 atcatggtat ggaaacctga cgagaatccg attaacccgg tcattccaac atcatacgga
    31141 ttatcgtatt cgacatgctc tttaccgcgt agggcatgaa caataggtgc tttaattttc
    31201 ccggcaaact caactaactc tttatgcgcc cccgcgcagc cgctgccaca catcagggcg
    31261 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggcatc
    31321 acgactggct gtggcgcatg ataccagtgc gtggttgccc cttctggcgc aggttttaac
    31381 gccacgtcgc ccggtaatac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    31441 gcaattgcca gcacttgtgg gatctgctcc gggctggaaa ccagctcgca atagtgacta
    31501 cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    31561 gagggaatat gagcggcaat cgccagtacc ggaacgttat tgcggtggca atcgaatagg
    31621 ccgttgatca agtgcaggtt gcctgggccg cacgatccgg cacagaccgc cagttctcct
    31681 gaaagttgtg cttcagcgcc agcggcaaac gccgccactt cttcatggcg ggtggacatc
    31741 cactcgatgg tgcccatgcg attaagactg tcgctaagac cgttcagaga gtcgcccgtg
    31801 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    31861 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    31921 acggaggggg aagggatggg agagaaagga ggcactaacg gttaaatagc ccgatgaaag
    31981 gaatatcatc gggcataagg cgattatgcg agaaccaaat ccccctgcgg atggcaggag
    32041 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt actgctcacc
    32101 gtatattcac cggaaaccac ttttgtctta cagcagccgc aaacacccgc acggcaggca
    32161 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggggcg
    32221 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttctgct
    32281 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgcttt cacttcctgc
    32341 tctacccaat ccatatacgg agccgggccg caggtcatca cggtacgtga agctaagtca
    32401 ggtacatctg ccagcagttc gcgagtgagg cgaccagcga taaagccttc ggtaacgtta
    32461 ttttctgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat aacatcctgc
    32521 ggcgtacgca cgttgtagat cacccgcaca tcggcctgtg gacggttctt cgcaagccag
    32581 cgacgcatcg acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct
    32641 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    32701 cgttttacat cgcgcgtcag ccactgggag ccgacaccgt catcaatccg ccgcacggtc
    32761 agggtgatat attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttcc
    32821 gctgagtttc gcacgctgac cagtgcatat tgcccggcgc gatatgggta gtgatcgtgg
    32881 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgtatc
    32941 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ttcaacagtt
    33001 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    33061 ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacgc
    33121 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    33181 gcgacagcgg cagatcgttc acaccacagc ccagtttctc tgccagagtg acagccagaa
    33241 taattgctga gtaagcatcg ttacattgac ctgcatctac cagacgcggc agaccttcga
    33301 tatcgccaaa ctcaagtttg ttaaagcgat atttaccaca ggcgagggtc aggatcaggc
    33361 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg gcgctcgccg cgtgcgccgt
    33421 cacagccacc aagcaggaag atatgacgca gtttttcacg gctaaccaga tcaatcagcg
    33481 tatcggctgc accaagcagc gtctggcgac caaagcccac ggtgatcagg tgaggaattt
    33541 cgctgtacgg gaagcccgcc atttgttgcg cctgggcgat gaccgcagag aaatcttcgc
    33601 catccagatg acgcacgcca ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    33661 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg ccagggaaac
    33721 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    33781 atttacgcag ctccgggtag ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    33841 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    33901 tcagaatgca tttacccgcc gtcgctttga cgttgacctg ggttggcgtc gggtgaccgt
    33961 atttaccggt ttcgcctgca tccagaatgc tcatcacttt gaagttcatc tggccgattt
    34021 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    34081 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    34141 tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt
    34201 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    34261 cgagatcatc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgc gcgttggcat
    34321 ctacagccag gcattgtgct ttcagcgcct cgcgcaaggc aatcgcttca cgagcgtagc
    34381 cgacaatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    34441 cgaagctgtc aacatcgtgg ttgatgatgc cgtattcacg cgcttttacc gcccaggcag
    34501 aaagcccttg cagcgccgcg atgagtaaat cctgaaggtc agaagtttcc gccgttttac
    34561 cacacatccc ctgcgcgtat gagcagccgt tttctgccgg agtacggata gtttgttcac
    34621 attgcacaca aaacatgatc acacctttta aagttatatt taatatacat gtttaagggt
    34681 aagacgctta acgcggggat aaaagggatt tttcatgcaa ctttaaggga gattgattta
    34741 gcgcaatttt ggcggcaggg aactaccgcc agagaggtat tacgcagaga aaaaggcgat
    34801 gaggatcggc actaacaggc taagaataaa accgtgaaca attgccgccg ggaccatatc
    34861 cagcccgcca gtacgttgaa gaacgggcag ggtgaaatcc attgatgtgg caccgcataa
    34921 gcccagtgca gtagagcggc tgcggcgaat cagcccaggg atcaacataa tagcaatcag
    34981 ttcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt
    35041 caataaaata ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt
    35101 gatggggaga tcaagaataa aggcgttaat taaaccacca attaatgaac tgacaaccac
    35161 caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt
    35221 attgcgcaac tgaataccaa cgaggaaaag tagcaaaatt aacgtgtatt cactggcttc
    35281 ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac
    35341 gccgcacagt tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg
    35401 atggtggttg cgccacggca ggcctcgctc cagccacatc agggcggcaa tattacacag
    35461 taaaataacg gtaatactga cggcagaata atgcagaatc gccaacaggt tactggcgag
    35521 gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct
    35581 taatagctga ttaatagctt ttaacgcagc ttgttggcga agcggaatga ggtaacccac
    35641 aatcagggga accagaatga ttaacagccc agaaaacatg aaaatccagt ccttgcaaag
    35701 aagaagtcga aatgcgcgat gacacactac tgaaagcgga agggcgagta aagttgcaat
    35761 taaaaggaaa tgttatgcat cagaggggg
//
LOCUS       DADLEE010000036        35113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000036 DADLEE010000000
VERSION     DADLEE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.036"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="JQH51_001849"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="JQH51_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY6076210.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1209..1871
                     /locus_tag="JQH51_001850"
     CDS             1209..1871
                     /locus_tag="JQH51_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6076211.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="JQH51_001851"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="JQH51_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY6076212.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /locus_tag="JQH51_001852"
                     /pseudo
     CDS             2405..2759
                     /locus_tag="JQH51_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2797..3102
                     /locus_tag="JQH51_001853"
     CDS             2797..3102
                     /locus_tag="JQH51_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY6076213.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="JQH51_001854"
     CDS             3105..3509
                     /locus_tag="JQH51_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076214.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="JQH51_001855"
     CDS             3499..3798
                     /locus_tag="JQH51_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076215.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3894..4280
                     /locus_tag="JQH51_001856"
     CDS             3894..4280
                     /locus_tag="JQH51_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY6076216.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLLTAFLFHSNFAEGVNSLMFMKNLT
                     ISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /locus_tag="JQH51_001857"
     CDS             4350..5336
                     /locus_tag="JQH51_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY6076217.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="JQH51_001858"
     CDS             5629..5997
                     /locus_tag="JQH51_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY6076218.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /locus_tag="JQH51_001859"
     CDS             complement(6204..7100)
                     /locus_tag="JQH51_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6076219.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="JQH51_001860"
     CDS             7205..7906
                     /locus_tag="JQH51_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY6076220.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /locus_tag="JQH51_001861"
     CDS             7929..8093
                     /locus_tag="JQH51_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076221.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /locus_tag="JQH51_001862"
     CDS             complement(8305..9615)
                     /locus_tag="JQH51_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY6076222.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="JQH51_001863"
     CDS             complement(9643..10974)
                     /locus_tag="JQH51_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY6076223.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="JQH51_001864"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="JQH51_001864"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY6076224.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="JQH51_001865"
     CDS             complement(12685..13074)
                     /locus_tag="JQH51_001865"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY6076225.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="JQH51_001866"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="JQH51_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY6076226.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="JQH51_001867"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="JQH51_001867"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY6076227.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="JQH51_001868"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="JQH51_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY6076228.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="JQH51_001869"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="JQH51_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY6076229.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="JQH51_001870"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="JQH51_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY6076230.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20219..20563
                     /gene="tdcR"
                     /locus_tag="JQH51_001871"
     CDS             20219..20563
                     /gene="tdcR"
                     /locus_tag="JQH51_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY6076231.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20819..21359
                     /locus_tag="JQH51_001872"
                     /pseudo
     CDS             20819..21359
                     /locus_tag="JQH51_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21381..21728
                     /locus_tag="JQH51_001873"
     CDS             21381..21728
                     /locus_tag="JQH51_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076232.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21927..22386
                     /locus_tag="JQH51_001874"
                     /pseudo
     CDS             21927..22386
                     /locus_tag="JQH51_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005089572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23055..23431)
                     /gene="rnpB"
                     /locus_tag="JQH51_001875"
     ncRNA           complement(23055..23431)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JQH51_001875"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23464..24609)
                     /gene="garK"
                     /locus_tag="JQH51_001876"
     CDS             complement(23464..24609)
                     /gene="garK"
                     /locus_tag="JQH51_001876"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY6076233.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24706..25596)
                     /gene="garR"
                     /locus_tag="JQH51_001877"
     CDS             complement(24706..25596)
                     /gene="garR"
                     /locus_tag="JQH51_001877"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY6076234.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25626..26396)
                     /gene="garL"
                     /locus_tag="JQH51_001878"
                     /pseudo
     CDS             complement(25626..26396)
                     /gene="garL"
                     /locus_tag="JQH51_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26412..27746)
                     /locus_tag="JQH51_001879"
     CDS             complement(26412..27746)
                     /locus_tag="JQH51_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076235.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28121..29692
                     /gene="garD"
                     /locus_tag="JQH51_001880"
     CDS             28121..29692
                     /gene="garD"
                     /locus_tag="JQH51_001880"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY6076236.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29744..30568)
                     /gene="agaR"
                     /locus_tag="JQH51_001881"
     CDS             complement(29744..30568)
                     /gene="agaR"
                     /locus_tag="JQH51_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY6076237.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30817..32097
                     /gene="kbaZ"
                     /locus_tag="JQH51_001882"
     CDS             30817..32097
                     /gene="kbaZ"
                     /locus_tag="JQH51_001882"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAY6076238.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32120..32593
                     /gene="agaV"
                     /locus_tag="JQH51_001883"
     CDS             32120..32593
                     /gene="agaV"
                     /locus_tag="JQH51_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY6076239.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32604..33383
                     /locus_tag="JQH51_001884"
     CDS             32604..33383
                     /locus_tag="JQH51_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY6076240.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33373..34182
                     /locus_tag="JQH51_001885"
     CDS             33373..34182
                     /locus_tag="JQH51_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY6076241.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34269..34703
                     /locus_tag="JQH51_001886"
     CDS             34269..34703
                     /locus_tag="JQH51_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY6076242.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34700..>35113
                     /locus_tag="JQH51_001887"
     CDS             34700..>35113
                     /locus_tag="JQH51_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY6076243.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9097 a         9063 c         8387 g         8566 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca ttactttcca aggtatcgtt attacaatta aacatcttta
    20161 aatatcaaca agttaaagca taaaaatcag cataaaccct gatttttatc aaaaaaaaat
    20221 gacggggata accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa
    20281 ggggctgaga ccatatttca agcaattacc ggataattac caggcaaaat ttgagttaaa
    20341 tttaatgagc aagttctcaa attttattat aaataaacca ttttcagcga taaatactgc
    20401 ggcacgtcac attttttcac gctatttgtt ggagaacaaa catttatttt atcaatattt
    20461 taaaatttcg aatacaggta ttgatcatct cgaacaattg attaacgtca actttttctc
    20521 ttctgacagg acgtcatttt gtgaatgcaa tcgttttcca taaattcttc tcccctcata
    20581 ggcgacgaat agcattttgt gttgaggatc acaaaacgaa taattgctgg tcgccgcgat
    20641 aaggtcagac gaagtcaaca agggggattt tcactgtgct ttacatcagc gtaggacaca
    20701 gaatgttgct tcgtttactg taacgccggg taaatgagcg ttttttgata gtgcgtaagt
    20761 gtcgtgcgag gaaaataagc atctattatt gtttgtcatg acgacaaggc aggcatcaat
    20821 gaaaggcttc ccaattgcac atatttttca cccttcaatc ccgccaatgc acgcagtggt
    20881 taacaatcac aatagaaata ttgattattg gacggtaaaa agaaagtttg cagaaattgt
    20941 ctccaccaat gacgttaata aaatttacag tataagtaat gaactccgga gagtattatc
    21001 tgcaattact gcattgaatt tctatcaagg cgatgttcct tctgtcatga ttccgaatcc
    21061 aaccggaaaa tatgagtcca ttcatcatag atatttctac aggagaacat gatgattata
    21121 tcatacaaac attagatgta ggcacttttg caccttttgg tgaacaatgt acttgctcag
    21181 ccgtcaataa aaaagagctg gaatgtatta aggagacgat ttctaagtat tgcgcaaagt
    21241 tcacccaaaa agaagccatc ttaacacccc ctaggcactt taataaaacc tgcattactt
    21301 cagattgttg gcaaattctc tttttttcac ccgatcattt taataatgat ttttattgat
    21361 tatttataaa ggataaaata atgttccctg tttcatcaat tggtaatgat attagtagtg
    21421 atttagtccg tcgaaaaatg aatgaccttc ctgaaagccc cataggaaat aacctcgaag
    21481 cactagcacc aggtatagaa aaactaaaac agacctctat tcaaatggtc actttactta
    21541 acgcgttaca accaagtgga aaatgcatta tcactggtga ttttcaaaaa gaattagcgt
    21601 acttacaaaa tgtaattctt tataatgact cgtctcttcg tctggatttt ttaggttata
    21661 acacccaaat tattcaacga tcggacaata cctgcgaact taccattaat gaaccgttaa
    21721 aaaactagga aatatctaca ggcaatatca atgttaattg cccattaaaa gatatttaca
    21781 atgaaatcag gaggttgaac gtaatcttta gttgtgggac tggagatata gttgatctat
    21841 cctctctgga cttacgtaat gtcgatttag attattatga tttcacagat aaacatatgg
    21901 ctaatactat tctaaatcat tttaaattgg attcaacaaa ttttactaat gccaacatgt
    21961 ttcaggttaa ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa
    22021 aaataacgcc tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg
    22081 tcgaaagttg tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat
    22141 ttgcaattat aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa
    22201 caaaagaaat atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac
    22261 cagagatgaa agaattctca tcaaaaaaaa catatgatta catcgataca atcattgaag
    22321 attatgaaaa tatcaaagaa aatgcttatc tggtcgtccc ccaaattaat tatactatgg
    22381 atttaaacat agaagactct agctcagaag agttaatacc ctcgagaaag acgaaaattc
    22441 tccggacaat ggctttgagg tcggggaata taacacatat gaagcatata actcagagaa
    22501 tcaatatttt accagagagg actatacgta taattacgac cttttaaatg caatatagac
    22561 ttaataactt cttacaaagt ctattattaa taccgtagat attcatctta ccctgtgcac
    22621 caaaagcaaa aacttatcga cgcgggtttg aaactgatgg caaccgcaaa aatgcctgat
    22681 gcgctacgct tatcaggcct acgccatctc tgcaatatat tgaatttgcg tgcttttgta
    22741 ggcaggataa ggcgttcacg cagcatccgg catgaataaa gcgcactcaa caatctgaaa
    22801 cccgccggaa cggtttatta cgtacatcag gtgaaactga ccgataagcc gctttctttt
    22861 gggtatagtg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg
    22921 aagcgggttt ttacgtatat caggtgaaac tgaccgataa gccgctttct tttgggtata
    22981 gcgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg
    23041 tttttacgta aaacaggtga aactgaccga taagccgggt tctgtcgtgg acagtcattc
    23101 atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt acgggccgta
    23161 ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac ggactgttac
    23221 cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg cttgcacggg
    23281 ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg cgttacctgg
    23341 caccctgccc tatggagccc ggactttcct cccctccacc cgtctccccc gaagaggacg
    23401 acgacgaagc ggcgactgtc tggtcagctt cgacgcgcag tatagagggt ttgcgcgccc
    23461 ttgtcacccc gtgttgcgca ttccaatcgc cagtgtcgcg gcaatattac gtgaagcacg
    23521 gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc tggtcaatac
    23581 gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg tcaggctacc
    23641 cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacgc caatcggtac
    23701 cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca gcgtacaatc
    23761 gtgaatatgt tcctccagat tcagcgccgt agtgacgatt tcaataccac ttttcagttc
    23821 cgcaccaaga aacgccatca gcgccgcacc cataccgccc gcagctcctg cgccggggac
    23881 atcttttaca tcaacatgca gcgctttttt aatgacatcg gcatagtgag agaggttatt
    23941 gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga tgcgcgatgc
    24001 gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga cgcaatcttt
    24061 taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac taccgccgcc
    24121 aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct gtaccatgcc
    24181 tgcgccgcca tcatttgtag cgctaccgcc aatgccgata ataatgtttg tcgcgccgct
    24241 ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga gtggatcgcg
    24301 tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa acgcggtttt
    24361 gccatcgccg gagatccccc aactggcatt cactttctcg cccagcggcc ctgtaaccca
    24421 ggcgtgacgt tcagaaccct gggtggctgc aatcatcgct tccaccgttc cttcgccacc
    24481 gtcggcaacc ggaatagaaa cgtactgtgc atcaggaaaa atttcccgaa atcctttttc
    24541 tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg gggcgattac
    24601 gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct tattcggcct
    24661 ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac gagtaacttc
    24721 gactttcgcc agtttttcgt agtagcacac cagggcgcta tgatccgccg ttcctaaacc
    24781 atctgctcgc agtgcctgca tcatctccat aaccgcagct gtgagcggca gttgcgcgcc
    24841 gacgccgtgg gaagtatcca gcgcatttgc cagatcctta atatgcagat caatacggaa
    24901 gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca cggtactgcc
    24961 cgccagtcca ccgcgaattg cctgatacac caggtccggg ttaacgcccg ctttagttgc
    25021 cagcgttaac gcttctgaca tcgcggcaat attcagcgcc acaatgacct gattcgccag
    25081 tttggtgacg ttacctgcac caattgcccc ggtatgcacc acggaacccg ccattgcttt
    25141 catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca ctgacagcgt
    25201 accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat caatgccttt
    25261 cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac tcatatcgat
    25321 caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg ccacctcttt
    25381 cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag cgatcgcttt
    25441 agccgtagag gctgtttctg cacctgcagc aatcacgtca gcaatagctt ctgggttacg
    25501 gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg gtttacccat
    25561 aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc ttgttgcggt
    25621 ggtggttatt ttttaaaggt atcagccagt ttatgagtgg cagagcggaa gacgccgaga
    25681 tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc atcggcttcg
    25741 accggcgcga ggataccgct gggtttgccg tgcgcgctgg cacggttaaa aatgtgctga
    25801 attgcttttt gtacatccgg gtgtgatgca ttgccgagat ggcctaatgc cgcggccaga
    25861 tcgctggggc cgacgaagat gccgtctacg ccttcggtag cggcaatggc atcgacgtta
    25921 tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt cgactgagcg
    25981 aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac gccgcgaatg
    26041 ccttccggtg ggtaacgggt tgatgccacc gcctgctctg cttcctcttt tgtttctaca
    26101 aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat ttccggctcg
    26161 ttggtcggca ctcacactac tggcgcgctg gcgctgcctt tcaaggccat taactgcgga
    26221 ataaacgtgg agatatcgtt tggcgcatgt tcgccatcca gcaccagcca gtcaaaccca
    26281 gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca accaatttgt
    26341 acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt attcatcgct
    26401 tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac gaagaggtag
    26461 cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt gaaggagtgc
    26521 agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac attgccaaag
    26581 acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc agaaatcacc
    26641 ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag catgaccacc
    26701 agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa cattcccagc
    26761 acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag ataatccgag
    26821 aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga ggcgaccaga
    26881 cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa ccaggtgagg
    26941 aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa catcatgcgg
    27001 ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc gctgcccggc
    27061 tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag ctcttcctca
    27121 gacatacgtg ggtgatctgt cgggttatga atcaacttga tccacagcgc cgtcagcaca
    27181 aaacaaatca cccccataac ggtaaagacg tgctcccatc cccaggcgaa agtcagccag
    27241 ccaagcagag gcgaaaagag cgccagcgag aaatattgtg ccgagttaaa gatggcggag
    27301 gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt cgccgggaag
    27361 gatggagctt ccgagaatcc cagcataaag cgcataaaga acatggagat ccctgcccaa
    27421 gccagcggga acatatcaac aaagccttgc aggaaggtga acagcgacca gaaaaagagg
    27481 ctgtaggtgt aaactttttt cgagccaaac ttatcaagca accagccgcc ggggatttgc
    27541 atcagcaagt aggcccagcc aaaagcggag aagatgtaac ccatcgaaac cgcacttaac
    27601 tgcaactctt ttgccacttc ggtaccagca atagacagcg ttgcacgatc ggcgtagtta
    27661 acggcggtaa caataaaaat aatcagtaat attaaatagc gggtatgcac acctttcctt
    27721 ttttcgtcaa cggtgtccag aatcatttta tttacctcgg gtacttatgc tgatttttat
    27781 tattatgggg aaggtgttat ttataagttt catttatgcc gtaacgacaa tgaactcggg
    27841 aattagtata agcagcgcga gaataataat cattgtgcaa atgctaattt aattaatact
    27901 ttttaaatat tattttgagc atatgcacat aaggttgcgc gctaaagcac agatttgcgc
    27961 tttaccttgc cgggcggcac tgcaatccct aaaatgattg acattgatca catttctgcg
    28021 tttaaactcc tgacattctt atttcaccca atgaaatcat ttatttttaa atgagaccag
    28081 gtcctcattt taataacccc tggctggaga atattgcaca atggccaaca tcgaaatcag
    28141 acaagaaacg ccaactgcgt tttatataaa agttcacgac acagataatg tggcaattat
    28201 tgttaatgat aatggcctga aagcaggaac gcgttttccg gatgggctgg aattaattga
    28261 acatattccc caggggcata aagtcgcatt gctggacatt ccggctaatg gtgaaattat
    28321 tcgttatggc gaagtgattg gttacgccgt gcgtgcaatc ccacgcggaa gctggatcga
    28381 cgaatcaatg gttgtactac cggaagcgcc gccgttacac acgctgccac tggcaaccaa
    28441 agtcccggaa cccttaccgc cgctggaagg atacaccttt gagggctatc gcaatgccga
    28501 tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc agcgtccact gtgtggcagg
    28561 cgtggtggac tatgtagtaa aaatcattga acgcgatctg ctaccgaaat acccgaacgt
    28621 cgatggcgtg gtggggctga atcatttgta cggttgtggc gtggcgatta acgcaccggc
    28681 agcagttgta cctattcgta ccattcacaa tatttcgctg aaccctaact ttggcggcga
    28741 agtaatggtg attggcctgg gttgtgaaaa gttgcagcct gagcgcctgc tgactggaac
    28801 ggatgatgtg caagctattc cagtagaaag cgccagcatt gtcagtttgc aggatgaaaa
    28861 gcatgtcggt tttcagtcca tggtcgagga tattttgcag gtcgccgaac gccatctaca
    28921 aaaactgaat caacgtcagc gagaaacctg tccggcttca gaactggttg tcggcatgca
    28981 ttgcggtggc agcgatgcgt tttctggcgt aacggcaaac ccggcggttg gctatgcgtc
    29041 tgatctactg gtgcgctgcg gcgcaacggt gatgttttca gaagtcacgg aagtgcgtga
    29101 cgcgatccat ctgctgacac cacgcgcagt gaacgaagag gtcggcaaac ggctgctgga
    29161 ggagatggag tggtacgata actatctcaa tatgggaaaa accgaccgca gcgccaaccc
    29221 ttcgccgggc aacaagaaag gcggtctagc aaacgtggta gagaaagcgc tcggctccat
    29281 tgctaaatcg ggcaaaagtg caattgttga agtgctgtcg cccggtcaac gcccgactaa
    29341 acgcggatta atttacgccg cgacgccagc cagcgatttt gtctgtggca cgcaacaggt
    29401 ggcttcgggt atcaccgtgc aagtgtttac gaccggtcgt ggtacgccgt acggcctgat
    29461 ggcggtaccc gtcattaaaa tggcaccccg caccgagctg gcgaaccgct ggtttgattt
    29521 aatggatatt aatgcgggca ccatcgctac cggcgaagaa actattgaag aggagggctg
    29581 gaagttgttc cactttattc tcgacgtcgc cagcgggaag aagaaaacct tctcggatca
    29641 atggggactg cataaccagc tggcggtgtt taacccagca ccggtgacct gataattcaa
    29701 ttcgtattat gcccgatagc acacgtttat cgggcatatt gagtcacagc gaagacgcgt
    29761 gatccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag
    29821 gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac
    29881 tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca
    29941 tccgacggct taaacgggct tcatcttcat tatgtgtgct gacgccgcgc tccagatcga
    30001 tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct
    30061 gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa
    30121 cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt
    30181 cagtgtactt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa tcgaggatca
    30241 cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg
    30301 tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga
    30361 tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac gccagatcat
    30421 tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc
    30481 cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcct gtcactcgct
    30541 tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt
    30601 tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattggcg
    30661 caagctactc tcgtttcagt gactttcatt acgtttcttt tgtgaatcag atcagaaaac
    30721 tattatcttt cgttttattt ttatctcacc atgacgcagt atcaattgaa acaaaacgaa
    30781 agatcaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg
    30841 agacagcaca aagcgggcaa aacaaatgca atttatgccg tttgttccgc acatccgctg
    30901 gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact gattgaagca
    30961 acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc cgattttcgc
    31021 ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgct gattctgggt
    31081 ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc aatggccaat
    31141 gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca ccttgattgc
    31201 agcatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc tgaacgcgcc
    31261 gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga agccgatctg
    31321 gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac cttaagcgag
    31381 ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg tcacgccttt
    31441 gaaaagcaag gtttgaatgc catctggcca cgcattattg ccctggtggt tcaacccggc
    31501 gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgag cgccttaagc
    31561 cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg
    31621 ccgcaatctc tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccggcg
    31681 ctgaccttcg ccctgcgtga agcccttttt tctctggcgg cgattgaaga agaactggta
    31741 ccagcgaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct cgaccgcccg
    31801 gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttac
    31861 agctactcag atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct
    31921 catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag ccagtatctg
    31981 ccgctgcagt acgtgaaagt tcgctccggc gagctgcaac cgacgccacg ggaactcatt
    32041 ataaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg ccaataagca
    32101 aaacaaagag gaacatgcta tgccaaatat tgttttaagc cggattgatg aacgcttgat
    32161 tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg tgctggtagc
    32221 caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga
    32281 agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc atcgcgccgc
    32341 cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga cgctggtgaa
    32401 aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca atggcaaaca
    32461 acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta acgacctgaa
    32521 agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt
    32581 taaattactt tgagggattc atcatggaaa tcagcctgtt gcaggcattt gcgttgggca
    32641 ttatcgcctt tatcgctggc ctagatatgt ttaacggctt aacccatatg caccgcccgg
    32701 tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga attttaaccg
    32761 gcgatacgct ggaactagtg tggatggggc tggccccgct ggcgggcgca cagccgccta
    32821 acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg
    32881 atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg attaccttcc
    32941 tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag aacgccgata
    33001 ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc
    33061 tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc
    33121 tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt
    33181 ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat accctacttc atcctgggct
    33241 ttgttgccgc aacctggctc aagttaccgg tgctggctat tgctgccgcc gcgctggcga
    33301 tggcgctgat cgatctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag
    33361 aggaatttga agatggcatc taatcaaacc accctgccga acgtcagcga aaatgaagaa
    33421 acactgctga ctggcgtcaa tgaaaacgtg tatgaagatc ggagcattgc cgcggagctg
    33481 acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac
    33541 tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct gaaaaagatc
    33601 cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc
    33661 catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg ttctaagcag
    33721 gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg cgggattggc
    33781 gatgcgatgt tctggctaac gctactgccg atttgtggcg ggataggcgc aagcctcgcg
    33841 ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt ggtacacttg
    33901 gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc gattcccctt
    33961 attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat gacggtaatc
    34021 ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac ggcgggcgac
    34081 gcagtagtta agttacaggc tgatgttatc gacaaactga tgcctgcctt cttaccgctg
    34141 gtctacaccc tgaccatgtt ctggctggta cgccgcggct agagtccgct gcgcctgatt
    34201 gcggttaccg tggttctagg gatcgtcggt aaattctgcc atttccttta aatacaatga
    34261 ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt ggcatggaaa
    34321 aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat tttcccgaaa
    34381 cctcaagcgc cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg
    34441 aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc gtagcttcaa
    34501 cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg caactgctac
    34561 tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta caggcgctgg
    34621 agtgtggaca ccgagggctg accagtctgg tggacgagtt aggccgctgc catgaagaat
    34681 gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga
    34741 gggatggttc gatgaccatc agttgcgtgt tgctgacggt gtcatcgcag caatcgaacc
    34801 gattccagtg ggcgtgactg aacgcgatgc ggaacggctc tgccccgctt acatcgacac
    34861 ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg tactcgacaa
    34921 gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca ccgtaaccgc
    34981 gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc aacgcggcgg
    35041 acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa
    35101 aggcgcgcat ccg
//
LOCUS       DADLEE010000037        34999 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000037 DADLEE010000000
VERSION     DADLEE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.037"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..815)
                     /locus_tag="JQH51_001888"
     CDS             complement(<1..815)
                     /locus_tag="JQH51_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6076244.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JQH51_001889"
                     /pseudo
     CDS             907..993
                     /locus_tag="JQH51_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JQH51_001890"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JQH51_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1398..1874)
                     /locus_tag="JQH51_001891"
     CDS             complement(1398..1874)
                     /locus_tag="JQH51_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY6076245.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="JQH51_001892"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="JQH51_001892"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY6076246.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="JQH51_001893"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="JQH51_001893"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY6076247.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="JQH51_001894"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="JQH51_001894"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY6076248.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="JQH51_001895"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="JQH51_001895"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY6076249.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="JQH51_001896"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="JQH51_001896"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY6076250.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="JQH51_001897"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="JQH51_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY6076251.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="JQH51_001898"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="JQH51_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY6076252.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="JQH51_001899"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="JQH51_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY6076253.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="JQH51_001900"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="JQH51_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY6076254.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="JQH51_001901"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="JQH51_001901"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY6076255.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="JQH51_001902"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="JQH51_001902"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY6076256.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="JQH51_001903"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="JQH51_001903"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY6076257.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="JQH51_001904"
     CDS             13849..14553
                     /locus_tag="JQH51_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY6076258.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="JQH51_001905"
     CDS             14758..15471
                     /locus_tag="JQH51_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY6076259.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="JQH51_001906"
     CDS             complement(15507..16463)
                     /locus_tag="JQH51_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY6076260.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="JQH51_001907"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="JQH51_001907"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY6076261.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="JQH51_001908"
     CDS             complement(17701..18462)
                     /locus_tag="JQH51_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY6076262.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="JQH51_001909"
     CDS             18595..19005
                     /locus_tag="JQH51_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076263.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="JQH51_001910"
     CDS             complement(18967..20073)
                     /locus_tag="JQH51_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6076264.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="JQH51_001911"
     CDS             complement(20084..21217)
                     /locus_tag="JQH51_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6076265.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="JQH51_001912"
     CDS             complement(21210..22946)
                     /locus_tag="JQH51_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6076266.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23937)
                     /gene="hlyD"
                     /locus_tag="JQH51_001913"
     CDS             complement(22939..23937)
                     /gene="hlyD"
                     /locus_tag="JQH51_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY6076267.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="JQH51_001914"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="JQH51_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY6076268.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26024
                     /gene="rhlE"
                     /locus_tag="JQH51_001915"
                     /pseudo
     CDS             24837..26024
                     /gene="rhlE"
                     /locus_tag="JQH51_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="JQH51_001916"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="JQH51_001916"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY6076269.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="JQH51_001917"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="JQH51_001917"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY6076270.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="JQH51_001918"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="JQH51_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY6076271.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /locus_tag="JQH51_001919"
     CDS             30114..31199
                     /locus_tag="JQH51_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY6076272.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31428..31688)
                     /locus_tag="JQH51_001920"
     CDS             complement(31428..31688)
                     /locus_tag="JQH51_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6076273.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="JQH51_001921"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="JQH51_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY6076274.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..33006)
                     /gene="ybiX"
                     /locus_tag="JQH51_001922"
     CDS             complement(32293..33006)
                     /gene="ybiX"
                     /locus_tag="JQH51_001922"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY6076275.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(33012..34999)
                     /locus_tag="JQH51_001923"
                     /pseudo
     CDS             complement(33012..34999)
                     /locus_tag="JQH51_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8973 c         9456 g         8275 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccatagtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgccgg
//
LOCUS       DADLEE010000038        34903 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000038 DADLEE010000000
VERSION     DADLEE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.038"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="JQH51_001924"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="JQH51_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY6076276.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="JQH51_001925"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="JQH51_001925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY6076277.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="JQH51_001926"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="JQH51_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY6076278.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="JQH51_001927"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="JQH51_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY6076279.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="JQH51_001928"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="JQH51_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="JQH51_001929"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="JQH51_001929"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY6076280.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="JQH51_001930"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="JQH51_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY6076281.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="JQH51_001931"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="JQH51_001931"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY6076282.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="JQH51_001932"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="JQH51_001932"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY6076283.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="JQH51_001933"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="JQH51_001933"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY6076284.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="JQH51_001934"
     CDS             12897..13847
                     /locus_tag="JQH51_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY6076285.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="JQH51_001935"
     CDS             complement(13888..14358)
                     /locus_tag="JQH51_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY6076286.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="JQH51_001936"
     CDS             complement(14413..15615)
                     /locus_tag="JQH51_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY6076287.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="JQH51_001937"
     CDS             complement(15702..16244)
                     /locus_tag="JQH51_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076288.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="JQH51_001938"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="JQH51_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY6076289.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="JQH51_001939"
     CDS             complement(16987..17589)
                     /locus_tag="JQH51_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY6076290.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="JQH51_001940"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="JQH51_001940"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY6076291.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="JQH51_001941"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="JQH51_001941"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY6076292.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="JQH51_001942"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="JQH51_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="JQH51_001943"
     CDS             21986..22876
                     /locus_tag="JQH51_001943"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY6076293.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="JQH51_001944"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="JQH51_001944"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY6076294.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="JQH51_001945"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="JQH51_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY6076295.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="JQH51_001946"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="JQH51_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY6076296.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="JQH51_001947"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="JQH51_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY6076297.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="JQH51_001948"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="JQH51_001948"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY6076298.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="JQH51_001949"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="JQH51_001949"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY6076299.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="JQH51_001950"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="JQH51_001950"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY6076300.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="JQH51_001951"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="JQH51_001951"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY6076301.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="JQH51_001952"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="JQH51_001952"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY6076302.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="JQH51_001953"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="JQH51_001953"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY6076303.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="JQH51_001954"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="JQH51_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY6076304.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="JQH51_001955"
     CDS             complement(33051..33512)
                     /locus_tag="JQH51_001955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6076305.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="JQH51_001956"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="JQH51_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY6076306.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="JQH51_001957"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="JQH51_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8539 a         9340 c         8647 g         8377 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat agagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttatcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DADLEE010000039        34788 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000039 DADLEE010000000
VERSION     DADLEE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.039"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..2098
                     /locus_tag="JQH51_001958"
     CDS             <1..2098
                     /locus_tag="JQH51_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6076307.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2551..3183
                     /locus_tag="JQH51_001959"
     CDS             2551..3183
                     /locus_tag="JQH51_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY6076308.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /locus_tag="JQH51_001960"
     CDS             complement(3271..4569)
                     /locus_tag="JQH51_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076309.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="JQH51_001961"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="JQH51_001961"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY6076310.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /locus_tag="JQH51_001962"
     CDS             6481..7968
                     /locus_tag="JQH51_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY6076311.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="JQH51_001963"
     CDS             8051..8602
                     /locus_tag="JQH51_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY6076312.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="JQH51_001964"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="JQH51_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY6076313.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="JQH51_001965"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="JQH51_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY6076314.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12699)
                     /locus_tag="JQH51_001966"
     CDS             complement(11683..12699)
                     /locus_tag="JQH51_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY6076315.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(12760..13452)
                     /locus_tag="JQH51_001967"
     CDS             complement(12760..13452)
                     /locus_tag="JQH51_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY6076316.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="JQH51_001968"
     CDS             complement(13529..14032)
                     /locus_tag="JQH51_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY6076317.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="JQH51_001969"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="JQH51_001969"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY6076318.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /locus_tag="JQH51_001970"
     CDS             15538..15852
                     /locus_tag="JQH51_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY6076319.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="JQH51_001971"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="JQH51_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY6076320.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /locus_tag="JQH51_001972"
     CDS             complement(16310..18328)
                     /locus_tag="JQH51_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY6076321.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="JQH51_001973"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="JQH51_001973"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY6076322.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="JQH51_001974"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="JQH51_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="JQH51_001975"
     CDS             complement(22127..23560)
                     /locus_tag="JQH51_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY6076323.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="JQH51_001976"
     CDS             complement(23623..24072)
                     /locus_tag="JQH51_001976"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY6076324.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="JQH51_001977"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="JQH51_001977"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY6076325.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="JQH51_001978"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="JQH51_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY6076326.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTCSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="JQH51_001979"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="JQH51_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30402)
                     /gene="ygjG"
                     /locus_tag="JQH51_001980"
     CDS             complement(28912..30402)
                     /gene="ygjG"
                     /locus_tag="JQH51_001980"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY6076327.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGVFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            30709..32229
                     /locus_tag="JQH51_001981"
                     /pseudo
     CDS             30709..32229
                     /locus_tag="JQH51_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(32383..33006)
                     /locus_tag="JQH51_001982"
     CDS             complement(32383..33006)
                     /locus_tag="JQH51_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY6076328.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="JQH51_001983"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="JQH51_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34082..34157)
                     /locus_tag="JQH51_001984"
     tRNA            complement(34082..34157)
                     /locus_tag="JQH51_001984"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="JQH51_001985"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="JQH51_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8485 a         8945 c         9141 g         8217 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tacaggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcacaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DADLEE010000040        34444 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000040 DADLEE010000000
VERSION     DADLEE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34444
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.040"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..331)
                     /locus_tag="JQH51_001986"
     CDS             complement(<1..331)
                     /locus_tag="JQH51_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076329.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="JQH51_001987"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="JQH51_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY6076330.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="JQH51_001988"
     CDS             complement(1692..2072)
                     /locus_tag="JQH51_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY6076331.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="JQH51_001989"
     CDS             complement(2086..2502)
                     /locus_tag="JQH51_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076332.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQH51_001990"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQH51_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY6076333.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="JQH51_001991"
     CDS             complement(3503..3970)
                     /locus_tag="JQH51_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY6076334.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="JQH51_001992"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="JQH51_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY6076335.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="JQH51_001993"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="JQH51_001993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY6076336.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="JQH51_001994"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="JQH51_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY6076337.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="JQH51_001995"
     CDS             complement(7712..8332)
                     /gene="rhtB"
                     /locus_tag="JQH51_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY6076338.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8443..9465
                     /gene="pldB"
                     /locus_tag="JQH51_001996"
     CDS             8443..9465
                     /gene="pldB"
                     /locus_tag="JQH51_001996"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY6076339.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9473..10273
                     /locus_tag="JQH51_001997"
     CDS             9473..10273
                     /locus_tag="JQH51_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY6076340.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10349..11248
                     /gene="bioP"
                     /locus_tag="JQH51_001998"
     CDS             10349..11248
                     /gene="bioP"
                     /locus_tag="JQH51_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY6076341.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11136..12089)
                     /gene="metR"
                     /locus_tag="JQH51_001999"
     CDS             complement(11136..12089)
                     /gene="metR"
                     /locus_tag="JQH51_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY6076342.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12325..14586
                     /gene="metE"
                     /locus_tag="JQH51_002000"
     CDS             12325..14586
                     /gene="metE"
                     /locus_tag="JQH51_002000"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY6076343.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14625..15440)
                     /locus_tag="JQH51_002001"
     CDS             complement(14625..15440)
                     /locus_tag="JQH51_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY6076344.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15702..16463
                     /gene="udp"
                     /locus_tag="JQH51_002002"
     CDS             15702..16463
                     /gene="udp"
                     /locus_tag="JQH51_002002"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY6076345.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16604..18031
                     /gene="rmuC"
                     /locus_tag="JQH51_002003"
     CDS             16604..18031
                     /gene="rmuC"
                     /locus_tag="JQH51_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY6076346.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18126..18881
                     /gene="ubiE"
                     /locus_tag="JQH51_002004"
     CDS             18126..18881
                     /gene="ubiE"
                     /locus_tag="JQH51_002004"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY6076347.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18895..19500
                     /gene="ubiJ"
                     /locus_tag="JQH51_002005"
     CDS             18895..19500
                     /gene="ubiJ"
                     /locus_tag="JQH51_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY6076348.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19497..21137
                     /gene="ubiB"
                     /locus_tag="JQH51_002006"
     CDS             19497..21137
                     /gene="ubiB"
                     /locus_tag="JQH51_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY6076349.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21216..21485
                     /gene="tatA"
                     /locus_tag="JQH51_002007"
     CDS             21216..21485
                     /gene="tatA"
                     /locus_tag="JQH51_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY6076350.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21489..22004
                     /gene="tatB"
                     /locus_tag="JQH51_002008"
     CDS             21489..22004
                     /gene="tatB"
                     /locus_tag="JQH51_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY6076351.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22007..22783
                     /gene="tatC"
                     /locus_tag="JQH51_002009"
     CDS             22007..22783
                     /gene="tatC"
                     /locus_tag="JQH51_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY6076352.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22825..23607
                     /gene="tatD"
                     /locus_tag="JQH51_002010"
     CDS             22825..23607
                     /gene="tatD"
                     /locus_tag="JQH51_002010"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY6076353.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="JQH51_002011"
     CDS             complement(23604..24092)
                     /gene="rfaH"
                     /locus_tag="JQH51_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY6076354.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24259..25752
                     /gene="ubiD"
                     /locus_tag="JQH51_002012"
     CDS             24259..25752
                     /gene="ubiD"
                     /locus_tag="JQH51_002012"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY6076355.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25798..26499
                     /gene="fre"
                     /locus_tag="JQH51_002013"
     CDS             25798..26499
                     /gene="fre"
                     /locus_tag="JQH51_002013"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY6076356.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="JQH51_002014"
     CDS             complement(26691..27854)
                     /gene="fadA"
                     /locus_tag="JQH51_002014"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY6076357.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="JQH51_002015"
     CDS             complement(27864..30053)
                     /gene="fadB"
                     /locus_tag="JQH51_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY6076358.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30243..31574
                     /gene="pepQ"
                     /locus_tag="JQH51_002016"
     CDS             30243..31574
                     /gene="pepQ"
                     /locus_tag="JQH51_002016"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY6076359.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31574..32188
                     /locus_tag="JQH51_002017"
     CDS             31574..32188
                     /locus_tag="JQH51_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY6076360.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32227..33678
                     /gene="trkH"
                     /locus_tag="JQH51_002018"
     CDS             32227..33678
                     /gene="trkH"
                     /locus_tag="JQH51_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY6076361.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33690..34235
                     /gene="hemG"
                     /locus_tag="JQH51_002019"
     CDS             33690..34235
                     /gene="hemG"
                     /locus_tag="JQH51_002019"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY6076362.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8697 c         9302 g         8337 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg tcacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc tgttgcggca
     7921 tgatgaattg cggaaatagc gccgccagaa acacaatact tttgggattg gtgagattca
     7981 caaaaactgc gcgctggaac aaatgtcgac gcgattgagt agaggccagc gatttaagat
     8041 caattgcgcc agcggcgcgc cactgctgga ttcccagcca aatcaagtaa gccgcgcctg
     8101 cccactttaa cacttcaaac gcaatcactg agcgggaaaa tagcgtcccc aaccccacgc
     8161 caaccagcac aatatgaatc gccagtccgg tctgaagccc agcaatagac gccaccgcgc
     8221 cgcgataacc gtggttgagc gaggtggtca tagtgttgat tgcaccagag cctggcgaca
     8281 ggcttaaaat gatcgatgtc agcaggtagg caaaccacca ttctaaggtc atgatgaact
     8341 cccggtgtgt ctatttttgt gccacaatac gctactgtcg cagcgttgtg tcaggcacgc
     8401 ttaaaaaaac gattttacgt ggtttaagag gcagattacc cgatgtttca gcagcaaaaa
     8461 gactgggaaa caagagaaaa cgcgtttgct gcttttacca tgggaccgct gactgatttc
     8521 tggcgtcagc gtgatgaagc agagtttact ggtgtggatg acattccggt gcgctttgtc
     8581 cgttttcgcg cacagcacca tgaccgggtg gtagtcatct gcccggggcg tattgagagc
     8641 tacgtaaaat atgcggaact ggcctacgac ctgttccatt tggggtttga tgtcttaatc
     8701 atcgaccatc gcgggcaggg acgttccggt cgcctgttag ccgatccgca tctcgggcat
     8761 gttaatcgct ttaatgatta tgttgatgat ctggcggcat tctggcagca ggaggttcag
     8821 cccggtccgt ggcgtaaacg ctatatactg gcacattcga tgggcggtgc gatctccaca
     8881 ttatttctgc aacgccatcc aggtgtatgt gacgccattg cgctaactgc gccaatgttt
     8941 gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga tcctcaactg ggccgaagcg
     9001 catccacgtt tccgtgatgg ctatgcaata ggcaccgggc gctggcgcgc gttgccgttt
     9061 gctatcaacg tactgaccca cagcagacag cgatatcgac gtaacttacg cttctatgct
     9121 gatgacccaa cgattcgcgt cggtgggccg acctaccatt gggtacgcga aagtattctg
     9181 gctggcgaac aggtgttagc cggtgcgggt gatgacgcca cgcaaacgct tctcttacaa
     9241 gctgaagagg aacgcgtggt ggataaccgc atgcacgacc gtttttgtga actccgcacc
     9301 gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa taaaaggtgc ttaccatgag
     9361 atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc acgccatcgt tgattttttc
     9421 aacaggcata actcacccag cggaaaccgc tctacagagg tttaaatttc ttatgtacca
     9481 ggttgttgcg tctgatttag atggcacgtt actttctccc gaccatacgt tatcccctta
     9541 cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc aactttgtgt ttgcgaccgg
     9601 tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg gagattaagt cttacatgat
     9661 tacctccaat ggtgcgcgcg ttcacgatct ggatggcaat ctgatttttg ctcataacct
     9721 ggatcgcgac attgccagcg atctgtttgg cgtagtcaac gacaatccgg acatcattac
     9781 taacgtttat cgcgacgacg aatggtttat gaatcgccat cgcccggaag agatgcgctt
     9841 ttttaaagaa gcggtgttca aatatgcgct gtatgagccg ggattactgg agccggaagg
     9901 catcagcaaa gtgtttttca cctgcgattc ccatgaacaa ctgctgccgc tggagcaggc
     9961 gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc tctaccttaa cctgtctgga
    10021 agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa gcagtggcga agaaactggg
    10081 ctacagcctg aaggattgta ttgcgtttgg tgacgggatg aacgacgccg aaatgctgtc
    10141 gatggcgggg aaaggctgca ttatgggcag tgcgcaccag cgtctgaaag atctgcatcc
    10201 ggagctggaa gttattggta ctaatgccga cgacgcggtg ccgcattatc tgcgtaaact
    10261 ctatttatcg taatcgttct ttatttggtc agttgtcaac ctgatacttc gctacaatgg
    10321 atactcgtta atcaaagagt tttccattgt ggcgttactt atcatcacca cgattctgtg
    10381 ggccttctcc tttagctttt atggcgagta ccttgcgggg cacgtcgata gctattttgc
    10441 ggtgctggtg cgcgttggct tggcggcact cgtttttctg ccgtttctgc gtacccgtag
    10501 caatagcctg aaaacggtcg gcctgtatat gctggtgggc gcgatgcagc tcggcgtgat
    10561 gtatatgctg agtttccgcg cttatctcta cctgacggtc tccgaattgt tgctgttcac
    10621 cgtactgacg ccgctctaca tcacgctgat ttatgacatc atgagtaagc gccgtctgcg
    10681 ctggggctat gcttttagcg ccttgctggc ggtgattggt gccgggatta ttcgctatga
    10741 tcaggtcacc gaccatttct ggactggctt gttgctggtg caactctcca atatcacttt
    10801 tgccatcggc atggtgggtt acaaacgcct gatggaaact cgcccgatgc cgcagtataa
    10861 cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg gtaattgcat ggttcttgct
    10921 gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg ggcattctgg tgtttcttgg
    10981 cgtggtggct tccgggattg gctactttat gtggaactac ggcgcgacgc aggtggacgc
    11041 cggaacgctg ggcattatga ataatatgca cgttccggca gggctgctgg taaacctggc
    11101 tatctggcac caacagccgc actggccaac gtttattaca ggcgcgctgg tgatcctggc
    11161 ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct tcacaaacgg cagatgatcg
    11221 caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa ctggctgacg ctgctcgcca
    11281 tcgcgcacgg cggcgtacag tcggctccac aagccttcgc ccagggtttt ggtcaccacc
    11341 agaccctggc gctcaaaact ctctactacc caatgcggta gcgcggcaat acccatccgc
    11401 gcggcaacca tttgaatcaa caataaggtg ttatcgacgc ttttcagtga cgggctgacg
    11461 cctgccggct gaagaaaatg ccgccagaca tccagtcgac tacgctgcac cggataaatt
    11521 aatagcgtct cgctggcgag atcttccggt gtaatacgcg tttttgccgc cagtggatgg
    11581 tcaggtgcta acaccaggcg tacttcataa tcgaacatcg gcgaataatg caggccactg
    11641 cgcggcagga tatcggaggt catcaccaga tccagctcgc cctgttgcaa cgcgggctgc
    11701 gggtcaaacg tcacgcccga tttaaaatcc atctctacct gcggccagtt cttatggaaa
    11761 tttcctaaag cgggtgtcag ccactgaata cagctatggc actcaatggc aatgcgcaga
    11821 cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc tgatttgcgg cagcacctgg
    11881 tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc gtagcggctg gctcttacgc
    11941 acaaatagcc ggaagccaag gcgttgttcc agatcgctaa actggtgaga cagggcggat
    12001 tgcgtctgat gcaacgttgc cgcagcggct gcgagcgagc cgcagttccg caacgcttgt
    12061 agcgttttca ggtgttttac ttcgatcatg aaagtccttc acttcggcat gaataatttg
    12121 cgcttgagga atatacagta accgccaatt atggatgtgt aaacatctgg acggctaaaa
    12181 tcattcgtct tttaaattta tggtgcgttg gctgcgtttc tccaccccgg tcacttactt
    12241 cagtaagctc ccggcgatga ataaacttgc cgccttccct aaattcaaaa tccataggat
    12301 ttacatataa ttagaggaag aaaaatgaca atattgaatc acaccctcgg tttccctcgc
    12361 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
    12421 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
    12481 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
    12541 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
    12601 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    12661 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    12721 ggccaacagt tcaaactgac ttggacgcag ctgctggagg aagtggacga ggcgctggcg
    12781 ctgagccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    12841 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    12901 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    12961 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    13021 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    13081 acgattactg cgctgcctgt tcaggggctg catgtggacc ttgtacatgg taaagataac
    13141 gttgttgaac tgcacaagcg cctgccttct gactggctgc tgtctgcggg tctgatcaat
    13201 ggtcgtaacg tctggcgcgc cgatcttact gagaaatatg cgcaaattaa ggacattgtc
    13261 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    13321 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    13381 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    13441 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    13501 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    13561 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    13621 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    13681 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    13741 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    13801 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    13861 ggttcccgct gcgtgaagcc accgattgtc attggtgaca ttagccgccc ggcaccgatt
    13921 accgtggagt gggcgaagta tgcgcaatcg ctgaccgaca aaccggtgaa agggatgctg
    13981 acggggccgg tgaccatact ctgctggtcg ttcccgcgtg aagatgtcag ccgtgaaacc
    14041 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg ccgatctgga agccgctgga
    14101 attggcatca tccagattga cgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    14161 gactgggatg cgtatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    14221 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    14281 tcgattgcgg cgctggacgc agacgtcatc accatcgaaa cctcgcgttc cgacatggag
    14341 ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa tcggtcctgg cgtctatgac
    14401 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccttgctgaa gaaagcggca
    14461 aaacgcattc cggcagagcg cctgtgggtc aacccggact gtggcctgaa aacgcgcggc
    14521 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa tttgcgtcgg
    14581 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    14641 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    14701 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    14761 tctgctttcg cattagcagc ccgcagcgcc tggcgcatcg tttcaacgct ctcctgcgga
    14821 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    14881 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    14941 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    15001 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    15061 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    15121 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    15181 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    15241 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    15301 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    15361 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    15421 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    15481 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    15541 gcagaatgaa gagtgattta tgtgatatga atcacttttg gtgggtaaat ttatgcaacg
    15601 catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    15661 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    15721 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    15781 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    15841 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgctcta
    15901 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    15961 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    16021 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    16081 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcta
    16141 ttggcgcgac aactcacgtt ggcgtgacag tttcttctga taccttctac ccaggtcagg
    16201 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    16261 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aactctgctg accatgtgtg
    16321 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    16381 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    16441 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    16501 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    16561 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    16621 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    16681 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    16741 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgtg aattgctcaa
    16801 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    16861 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    16921 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    16981 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    17041 tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    17101 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    17161 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    17221 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    17281 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg
    17341 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    17401 ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    17461 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    17521 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    17581 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    17641 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    17701 taacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    17761 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    17821 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    17881 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    17941 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    18001 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    18061 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    18121 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    18181 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    18241 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    18301 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    18361 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    18421 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    18481 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    18541 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    18601 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    18661 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    18721 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    18781 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    18841 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    18901 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    18961 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    19021 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    19081 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    19141 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    19201 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    19261 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    19321 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    19381 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    19441 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    19501 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    19561 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    19621 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    19681 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    19741 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    19801 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    19861 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    19921 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    19981 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    20041 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    20101 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    20161 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    20221 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    20281 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    20341 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    20401 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    20461 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    20521 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    20581 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    20641 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    20701 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    20761 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    20821 gtattcctgc tctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    20881 cagaactgcc tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    20941 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    21001 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    21061 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    21121 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    21181 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    21241 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    21301 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    21361 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    21421 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    21481 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    21541 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    21601 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    21661 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    21721 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    21781 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    21841 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    21901 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    21961 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    22021 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    22081 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacttggt
    22141 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    22201 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    22261 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    22321 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggca tggcattcgc
    22381 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    22441 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    22501 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    22561 gtcagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    22621 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    22681 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    22741 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    22801 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    22861 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    22921 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    22981 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    23041 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    23101 ggtctcgact ttaaccgtaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    23161 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    23221 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    23281 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    23341 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    23401 attccggcgg agaaattgct gatcgaaaca gatgcgccgt atctgctccc gcgcgatctc
    23461 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    23521 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    23581 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    23641 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    23701 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    23761 taaggggggg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaattacc
    23821 gccgatggga ctatcgctgg cgacgcgcca aagcgcacaa agtggctgac gccacgcgta
    23881 gcgctgatgg tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttaggg
    23941 aataacggtt cactgactgc ggtacgttta ccccgtacga ttttttccag ggtgatcatc
    24001 ggagcgaggc aattaaccgc ctgtctctca aggtgttcct gggcacgttg aagttgcccg
    24061 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    24121 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    24181 agagttcacg ctaatttaac aaatttacag cattgcaaag atgaacgccg tataatgggc
    24241 gcagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg
    24301 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    24361 atcactgaaa ttgctgaccg cactctgcgt gctggtgggc ctgcgctgtt gttcgaaaac
    24421 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg
    24481 atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt
    24541 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    24601 aagcaggtgt taaacatgcc gacaaagcga ctgcgtggtg cgccctgcca acaaaaaatc
    24661 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    24721 gccgcgccgc tgattacctg ggggctgacc gtaacgcgcg gcccgcataa agagcggcag
    24781 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    24841 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    24901 ttcccggttt ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc
    24961 gttccggata cgctttcaga gtatgcgttt gccggattgc tacgcggcac caaaaccgaa
    25021 gtagtaaagt gtatttccaa tgacctcgaa gtgccagcca gtgccgaaat cgtcctggaa
    25081 gggtatatcg atcctggtga gatggcgccg gaagggccgt atggtgacca cacaggttac
    25141 tataatgaag tcgataattt cccggtgttt accgtgacgc atattaccca gcgtgaagat
    25201 gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt actgggcgtg
    25261 gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat tgtcgatttt
    25321 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    25381 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    25441 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    25501 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    25561 attgattatc tggattttgc ctcgcctgtc tctgggctgg gttcaaaaat ggggctggat
    25621 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    25681 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    25741 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    25801 acaaccttaa gctgtaaagt gacctcggta gcagctatca cggataccgt atatcgtgtc
    25861 cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg
    25921 gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa agggtttatc
    25981 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    26041 ctcaaagatc atcaaatcgt ggtcgacctt ccccacggag aagcgtggct gcgcgatgat
    26101 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    26161 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    26221 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa gcaccttggt
    26281 ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg tactggtacc
    26341 gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc
    26401 ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg taatgcgcgg
    26461 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    26521 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    26581 ccggcaattg gtgcacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    26641 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    26701 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactga
    26761 acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc
    26821 agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc aatctgctcc
    26881 attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc gttcatctca
    26941 aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga
    27001 accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacacga
    27061 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    27121 gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac tggatcaaac
    27181 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    27241 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat ttcattttta
    27301 aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    27361 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    27421 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtgactcatc
    27481 ggcacatggc ctatatgctc cacgccgcca accagacatg cctgcgtatc gccagtcatg
    27541 atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacggta
    27601 accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc gatgttaaaa
    27661 ccctgctcca gcgtctgttg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    27721 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc
    27781 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    27841 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    27901 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    27961 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    28021 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    28081 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
    28141 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    28201 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
    28261 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    28321 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
    28381 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
    28441 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
    28501 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
    28561 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
    28621 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
    28681 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
    28741 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
    28801 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
    28861 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
    28921 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
    28981 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    29041 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
    29101 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    29161 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
    29221 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
    29281 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg cgcgaccatt
    29341 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
    29401 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
    29461 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    29521 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
    29581 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    29641 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    29701 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    29761 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    29821 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    29881 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    29941 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    30001 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    30061 tcctgaatcc gcaaggtaat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
    30121 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    30181 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    30241 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
    30301 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
    30361 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    30421 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
    30481 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
    30541 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    30601 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
    30661 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
    30721 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
    30781 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
    30841 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    30901 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    30961 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    31021 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    31081 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
    31141 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    31201 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    31261 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
    31321 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
    31381 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
    31441 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    31501 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
    31561 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    31621 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    31681 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    31741 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
    31801 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
    31861 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    31921 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    31981 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
    32041 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    32101 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    32161 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    32221 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    32281 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    32341 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    32401 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
    32461 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    32521 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    32581 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
    32641 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    32701 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    32761 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    32821 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    32881 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
    32941 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    33001 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    33061 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
    33121 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
    33181 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    33241 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
    33301 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    33361 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    33421 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    33481 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    33541 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    33601 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
    33661 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    33721 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    33781 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
    33841 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    33901 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    33961 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    34021 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    34081 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    34141 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    34201 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
    34261 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    34321 actgacacgg aacaacggca aacacgcagc cgggtcagcg gggttctcct gagaattccg
    34381 acagagaaag caaaaataaa tgcttgactt tgtagcggga aagcgtatta tgaacaccct
    34441 gcgc
//
LOCUS       DADLEE010000041        33975 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000041 DADLEE010000000
VERSION     DADLEE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33975
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.041"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..344)
                     /locus_tag="JQH51_002020"
     CDS             complement(<1..344)
                     /locus_tag="JQH51_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6076363.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVR"
     gene            1697..2476
                     /gene="ipaJ"
                     /locus_tag="JQH51_002021"
     CDS             1697..2476
                     /gene="ipaJ"
                     /locus_tag="JQH51_002021"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY6076364.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3423..4352)
                     /locus_tag="JQH51_002022"
     CDS             complement(3423..4352)
                     /locus_tag="JQH51_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY6076365.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4569..4805)
                     /locus_tag="JQH51_002023"
     CDS             complement(4569..4805)
                     /locus_tag="JQH51_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY6076366.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4809..6710)
                     /gene="ipaA"
                     /locus_tag="JQH51_002024"
     CDS             complement(4809..6710)
                     /gene="ipaA"
                     /locus_tag="JQH51_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY6076367.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6719..7717)
                     /gene="ipaD"
                     /locus_tag="JQH51_002025"
     CDS             complement(6719..7717)
                     /gene="ipaD"
                     /locus_tag="JQH51_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY6076368.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7768..8859)
                     /gene="ipaC"
                     /locus_tag="JQH51_002026"
     CDS             complement(7768..8859)
                     /gene="ipaC"
                     /locus_tag="JQH51_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY6076369.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8879..10621)
                     /gene="ipaB"
                     /locus_tag="JQH51_002027"
     CDS             complement(8879..10621)
                     /gene="ipaB"
                     /locus_tag="JQH51_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY6076370.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10627..11094)
                     /gene="ipgC"
                     /locus_tag="JQH51_002028"
     CDS             complement(10627..11094)
                     /gene="ipgC"
                     /locus_tag="JQH51_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY6076371.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11151..11777)
                     /gene="ipgB"
                     /locus_tag="JQH51_002029"
     CDS             complement(11151..11777)
                     /gene="ipgB"
                     /locus_tag="JQH51_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY6076372.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11793..12182)
                     /gene="ipgA"
                     /locus_tag="JQH51_002030"
     CDS             complement(11793..12182)
                     /gene="ipgA"
                     /locus_tag="JQH51_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY6076373.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12195..13679)
                     /gene="icsB"
                     /locus_tag="JQH51_002031"
     CDS             complement(12195..13679)
                     /gene="icsB"
                     /locus_tag="JQH51_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY6076374.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13993..15609
                     /locus_tag="JQH51_002032"
     CDS             13993..15609
                     /locus_tag="JQH51_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY6076375.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15619..15981
                     /gene="ipgE"
                     /locus_tag="JQH51_002033"
     CDS             15619..15981
                     /gene="ipgE"
                     /locus_tag="JQH51_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY6076376.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15981..16439
                     /gene="ipgF"
                     /locus_tag="JQH51_002034"
     CDS             15981..16439
                     /gene="ipgF"
                     /locus_tag="JQH51_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY6076377.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16448..17563
                     /gene="mxiG"
                     /locus_tag="JQH51_002035"
     CDS             16448..17563
                     /gene="mxiG"
                     /locus_tag="JQH51_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY6076378.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17571..17822
                     /gene="mxiH"
                     /locus_tag="JQH51_002036"
     CDS             17571..17822
                     /gene="mxiH"
                     /locus_tag="JQH51_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY6076379.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17835..18128
                     /gene="mxiI"
                     /locus_tag="JQH51_002037"
     CDS             17835..18128
                     /gene="mxiI"
                     /locus_tag="JQH51_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY6076380.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18134..18859
                     /locus_tag="JQH51_002038"
     CDS             18134..18859
                     /locus_tag="JQH51_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY6076381.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18856..19383
                     /locus_tag="JQH51_002039"
     CDS             18856..19383
                     /locus_tag="JQH51_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY6076382.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19325..20020
                     /locus_tag="JQH51_002040"
     CDS             19325..20020
                     /locus_tag="JQH51_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY6076383.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20013..20420
                     /locus_tag="JQH51_002041"
     CDS             20013..20420
                     /locus_tag="JQH51_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY6076384.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20404..20832
                     /gene="mxiM"
                     /locus_tag="JQH51_002042"
     CDS             20404..20832
                     /gene="mxiM"
                     /locus_tag="JQH51_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY6076385.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20962..21594
                     /gene="mxiE"
                     /locus_tag="JQH51_002043"
     CDS             20962..21594
                     /gene="mxiE"
                     /locus_tag="JQH51_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY6076386.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21581..23281
                     /gene="mxiD"
                     /locus_tag="JQH51_002044"
     CDS             21581..23281
                     /gene="mxiD"
                     /locus_tag="JQH51_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY6076387.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23300..24367
                     /gene="mxiC"
                     /locus_tag="JQH51_002045"
     CDS             23300..24367
                     /gene="mxiC"
                     /locus_tag="JQH51_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY6076388.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24380..26440
                     /gene="mxiA"
                     /locus_tag="JQH51_002046"
                     /gene_synonym="virH"
     CDS             24380..26440
                     /gene="mxiA"
                     /locus_tag="JQH51_002046"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY6076389.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26453..26854
                     /gene="spaK"
                     /locus_tag="JQH51_002047"
     CDS             26453..26854
                     /gene="spaK"
                     /locus_tag="JQH51_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY6076390.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26858..28150
                     /locus_tag="JQH51_002048"
     CDS             26858..28150
                     /locus_tag="JQH51_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY6076391.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28243..28581
                     /gene="spaM"
                     /locus_tag="JQH51_002049"
     CDS             28243..28581
                     /gene="spaM"
                     /locus_tag="JQH51_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY6076392.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28568..29446
                     /gene="spa32"
                     /locus_tag="JQH51_002050"
     CDS             28568..29446
                     /gene="spa32"
                     /locus_tag="JQH51_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY6076393.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29440..30321
                     /gene="spaO"
                     /locus_tag="JQH51_002051"
     CDS             29440..30321
                     /gene="spaO"
                     /locus_tag="JQH51_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY6076394.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30326..30976
                     /gene="spaP"
                     /locus_tag="JQH51_002052"
     CDS             30326..30976
                     /gene="spaP"
                     /locus_tag="JQH51_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY6076395.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30991..31251
                     /gene="spaQ"
                     /locus_tag="JQH51_002053"
     CDS             30991..31251
                     /gene="spaQ"
                     /locus_tag="JQH51_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY6076396.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31251..32021
                     /gene="spaR"
                     /locus_tag="JQH51_002054"
     CDS             31251..32021
                     /gene="spaR"
                     /locus_tag="JQH51_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY6076397.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32028..33056
                     /gene="spaS"
                     /locus_tag="JQH51_002055"
     CDS             32028..33056
                     /gene="spaS"
                     /locus_tag="JQH51_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY6076398.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33069..33311
                     /locus_tag="JQH51_002056"
     CDS             33069..33311
                     /locus_tag="JQH51_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076399.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10569 a         4978 c         6829 g        11599 t
ORIGIN      
        1 cgaacggcag gctcctgctc ctgaggaacc gagagagaac gaatgaatgc cctgagaatg
       61 gtcattccgc cacgatatcc ctggtctctg atctcacgag cgattaccgt tgccgggatt
      121 ttgtaaggat gagcatcggc gatgcgttga cgaatataat cccggtattc atccaggagt
      181 gaagcaacag caggtcgcgg cgtatatttt ggcggctcag attttgcctg caaataacgt
      241 ttaacggtat tgcgggagag ccccagttct ctggcaatcg tccggctact cattccctgc
      301 ttgtgcagga ttttaatttc cataactgtc tcaaaagtga ccataagctc tcctgaatca
      361 ggagagcaga ttaccccctg gatctgattt caggcgttgg gtgtggatca ctattgcacc
      421 gttcgtgaca cttgttgact acctgtgcgg catctggtat caaggtggcg tgaagccggt
      481 tcaggttctc atgcggaacg ttggaaccta tatccccccg ataccaaagg cgaaatgtaa
      541 gtgacaaaat gcaatgaaca cgttctccgt taactgagga gcatggtgat aaataaaggt
      601 aaatgcagaa ggtgtagagt ctcgcagatt tctgacatca tagagcctaa cattagatgc
      661 ataacgctac agattgtaga atctctacag gtgaaatata cactggttgg cgtttatgtc
      721 gcaaaacatg tcattcaggt tcatctcacc aataaggata tgagtgaagt ggaggataag
      781 tgagtctgct gtcagagttt tctggtgtat gtcagtaact gcgaaccatg tctgcttgat
      841 atgggagctt atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa
      901 gtccgcctga tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga
      961 tgaatgttca ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg
     1021 taacaataga agaactgcag gcagtactgg tcccacatcg tttgctcagt caactggtga
     1081 agttccgtag cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat
     1141 acctgcatat atcattactg cgttggatta ccagtacaac agattgaatg aattggacat
     1201 gttgatagac ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag
     1261 cacttcgcct tctgagtatt aagtaagcat ggtggtacct gcggatacta tctattcact
     1321 gtgctcggtc ggcagcctta ctgaccaagg agttggggga gttgataaca aagttgaaaa
     1381 aacagctctc aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat
     1441 cacaataacc gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa
     1501 ttgctgatgc tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg
     1561 gtgacggacg tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg
     1621 gaataaggac ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg
     1681 ctgcaattac tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac
     1741 aggtgttatg ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc
     1801 tcatcagact gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc
     1861 gaggaagcag atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga
     1921 attaggggtt gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag
     1981 ttctcttgat ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta
     2041 taatccgaga atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat
     2101 agtcatggct acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt
     2161 ttcgcaagta attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg
     2221 taatatagta catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc
     2281 tgtatccttt attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag
     2341 ctatatggat ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc
     2401 aagaagaagt aattctaact ttataggata tactaaaata ggtatttcca tagttataac
     2461 taatgaggct ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggtg
     2521 gggggcataa caaatgatat tactgagtac aaatttagct atattgggcg gattagttgt
     2581 gtgggaagac gaaatgtttc tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc
     2641 ttaaatattt aagtgctggt aagtttgaga aggctgacat attttgcaga gcaaacagtg
     2701 attgacttgg atgatatttt tattttagtc actcgttgta attggagtat gctttatttt
     2761 atttgaaaat agatgtattt tatacccgca atttaaactg taagccctat gaggggtatg
     2821 gatattataa gttactcttt gaccaagcat aacttttggg gttagaaaaa taaatctctt
     2881 gttaataatt aatatgattt taaaatcaaa cttgtttata attataaaat aatgtaagtc
     2941 ggtcatttta tgggaagatt aggagaataa aacagtaatt atatttgaga aatctgctaa
     3001 tatattctta aagctttttt gatactgtga acgaagtctg ttaatggtgg attttttgtt
     3061 tatcaccatg taaaaaaaat tgagtagtaa aagcgttgac tgctaatcct cgctgttttt
     3121 attgaaacga ttaaaaatac gctgaatatt ttcggccagt cactcatatt cacataaaat
     3181 tttgaccatc atgccgcatc ccttagttgc acaattcatt ttcaatgata tgaatattac
     3241 taatttcacc agataatttt ctcgtggtca gcagggtatg agttttcacc gtaattttaa
     3301 ggaaacacag tgcgtgggga ttgaataaca gatagccgac ggaattaccc caccggctga
     3361 aagtcaagtt caacgaagat ttttattatc tgaattgggc agtttacatc agtgttcgat
     3421 gtttatgaag acgatagatg gcgagaaatt atatcccgaa tagcttcatc tatttctctc
     3481 tgaactgttt tgtttatttt agataatgta aatattagag ttctgtttgt tttattctct
     3541 tttcttttta tgtaagtccg tttgtcttta gaaataaata acgagtcaac ttttagcgaa
     3601 gggttttgct ttctgttttt gcttttcttt atgatgttta aaatttcttt tttatatatg
     3661 tccgggggca aatttgtatc aaggtctttt atttcttcct ttaatattgg tagtgtagaa
     3721 ctaagagatt cattagcctt ttcaagtcct ttataataat tgaataatat cttgtagtca
     3781 ttaaagttaa gttctgacgc gattggaaat agagatatta tttcctgtgg aacgcttgct
     3841 gcctgaaagg cacgagtgac tttcgcgcga gacagattct cttttttggc tatgtcttta
     3901 taggacatcc ctgatacttt cagaaaatta agaccaatac caagttctcg gatgctatgc
     3961 tcttttgctg tttgtatatc gtttgctagt tttctggcat caagagtaga tatatattct
     4021 tttgaatata gaacttcaag atctgctcct gcatatattg cagatgctct tctacgagtg
     4081 ccatccagaa tttcaattct accatcaatc tcccttccta ttacagggaa gaattgttgt
     4141 agctttatag tttttatgat atcggctaat gattcttctg ttagggattc ttgatcgcgt
     4201 acattcgttt tttggttgac gaaggttaaa tcttggatcc ttttatgagg aatcttggct
     4261 ttgataaagg aaactttatt accagaatgg agtgtaaact ctttcttttg gccttccttt
     4321 atacttaaca agtcgttgca caaatccacc atatcacacc ctgtttattc atattgattt
     4381 atattgacaa aagttaaatg cagtgtgagc aagattgtat gtaactgttg atgatagtca
     4441 acggtagtct taaaaattac tattttaata gctggattga tgcaatttat ctttattgga
     4501 taaaagaata atattcttgt ggagctctga tgtgagagct ccatagatga aaaaatgctt
     4561 atcaattact atattgattt tgtactattt ttgactacag aaataagatc tccaatagta
     4621 ataatgtgtt ctaatgtaga ttcatctata cgtagattga actcaaaact aatcatatcc
     4681 agcatttcaa tcagagaaag agaatcggca gaaagatctt caataaattt agtttcttca
     4741 gatagtttgc tgtacgctat accatagcaa aaggccacta ttgataatat tttttctttt
     4801 atcatatttt aatccttatt gatattcttt aatacttttg atagggaagt ggttacatct
     4861 tttgctgctt tatatatatt attgttttca gctgtaatat cattgttatt ttttaaatca
     4921 gatatatcat ctgtaagtaa ttctgcagag gtatcgtcaa tttttgacaa taccttgctg
     4981 agggcgctag atacttcttt tgccttttca tagatcgcat gatttttatc aattgtatct
     5041 gcgttggttt ctgttacgta gtttcttata tttgcatctg taacttttaa tttgttgttt
     5101 attctatgtt ctgttgtgga ttttgtaatg tcctttgatt tttcctgtaa ctctcgttct
     5161 tgtgttcctg atttagttcc aatgagagat ataagattac ttagggagtt tgttactttt
     5221 tttgaagctt caaatatcgt ttcccgtgtt aaatatgtgt ctccatttct tgataaaaaa
     5281 tcagtctgaa gtacttttga atcagtgttt gagttgaatt tattgatttg cgaagctact
     5341 ctatgagaaa gagaagcgct acttttagtt gtatctgatg agttatcatt actgcttgtc
     5401 actccttccg ggtggagagg gggggtggtt gaagagttct gtatgttcag aggcctgtgt
     5461 ccccgagaaa gagagttatt tgcagatctt tcttgactac tcgtagttaa caggtctgaa
     5521 cctattccat ccgaattatc tggagtatgg ttgaaagcct tttctaaagc tgaattattt
     5581 gcaaatgtct ggacagtatc tgatatagag tgtggtagaa cggaagttac tttttgaact
     5641 agtgctcttt gtggatttag taatgagttt ccgttacggg ataatagatc gtttgtctga
     5701 gaagtaggag actgtgcatc attttctggg ttctcgtgat agctattgtc atatactctg
     5761 ttgtcaaaaa ctctattatc gtaagttcta ttatcattat ttatatgata gtgaattaca
     5821 ggttgggatg ttttctcatt ggctccgggg gttggtccgc catctggcat atcttcggga
     5881 ggtgtaggga ggccatcttg gataggatgc gaatctttta tatcttctaa aatatcatta
     5941 aggacttttc tcaacagctc aggaaacacg gctactgtat ttaagtctct tgacagttta
     6001 cctataccaa atcctttact atcaatagta tttttacgtt gattgcgcaa actatcaaag
     6061 gcaagttttg cctgtgcgtc aactaaatca tctagtttga gagatgaaac agtttcatta
     6121 cttaacttta attcagcaag aatattattc ttgataaatg atgatacttc agtattgagg
     6181 agtttttgtc ccaatgataa agcttcacca gatgtgtgtt gttgtgctaa tagtgcaagt
     6241 ctgtgctcaa tgttttgtcg taattgatgg agtttcttat tctttgccga ccatggtcca
     6301 aaggcagaat cttgggataa tttatctttt ataattagtt ccgaatactg tgcgacaatt
     6361 ttttgaagtc ccttttcgtt ttcaaaatta agaccatttt gatgtactaa ggagtacaaa
     6421 acatttgaaa attcgagaat ctcgcttttg gcatacgcat tcattgagtt ggaataaatg
     6481 cttcttaaca catcttcttt ccctgaggaa gaaaataaaa aatcaagaca tttctgatta
     6541 aaagacgttg caaagttttc tttttcagac actgatgctg gtgtttttag agaagtttgc
     6601 gaactatgga tatcggatat tttgcttttt aactcgctgt attctgttga tgatggtgaa
     6661 gttgccttat ataagaatgt tggcgcttga gtattattta cattatgcat ggcgcacctc
     6721 agaaatggag aaaaagttta tctgtatctg tacatgagct tattgtacta ctcaaaacct
     6781 ttactaaatt atcaaaaata ctattggcat tactgtattt ttgaactaaa gtttgaagat
     6841 tatttttcat tgtttcatct tcggcagaga atccggcatt ccatgcctga tattttgcat
     6901 tatctagcac aacctcgcca tttccaccta gattatctaa gctttttaac atattgtcta
     6961 ttggggtcat gtttatactg acaacatatc ccccgttttt ttgagatacc ttgccgattg
     7021 ttccacctaa ttctgtaagc catttatttg cttgttcctg actaacagta ttatttgctg
     7081 gatatagcgg tttatcttta tatttttcct tgagttcttc caatgccttt ttaagcgagt
     7141 tgacttgtaa tttcacggag tttccgtcgt tacctccggg agagatccag ccggcaagac
     7201 tggaaagaac agcgctaaaa tcttgataca tttgagtata tgaactaacg gcatgttcat
     7261 atactttcag atactgttca ttaatatcat tgatggagtt tgcaatttta gcccacagtt
     7321 ctctatgaga tatcatttga tctccatcaa gctcggcttc tttcggagct gaatgtaata
     7381 attctcttgc gtctttatta attggatatt cgtgcctgga aagaatatct aatagttgag
     7441 cggatttatt tacatccatg ctaatctgag atgaatgtaa tgctatttct tctagcgatg
     7501 ttttagtcaa cgttttttgt gaaagctctt tctttaatgc ctgatttgtt gttctgatat
     7561 tatgaagggt gtcgttgagc atagtaaggg aacttacagg atgagaactg gtcgttgttt
     7621 ttatatcaga attaactgtt tcggttgatg aaccgttggt attgtttgga ctgaatgatg
     7681 aggtggaaat actattagtc agagttgtta tattcatggt tatttcctta caggaagagc
     7741 catatattat tatggctctt ggatatatta agctcgaatg ttaccagcaa tctgactggc
     7801 tgtcgaattc tttgattggt tgatgctgtc aattatattc aataattttt gtattaattg
     7861 atttgtcgct tgggatgctt ctttagatac ttgggatgct tcctctgctt gtttactgct
     7921 ggcctgactg attagttgtt cttcttcttc aagagcagat gcataacgcc ctcctgatgt
     7981 ggatatatta ccagcaacgg ctgatgtttc cattgttgca cggccaatgt tttgtttttg
     8041 ctgcgcagaa agggtattga gctcgtaagt tcttctctgg gtgtcaattt tatcctgcaa
     8101 agaaatatcg ggagaactaa gagaagtttt atgttcagtt gacagggata tattatctgg
     8161 cgccagttta tttttaccta aaaattttgt gctagagtta gtttgtggtg aggtgatatt
     8221 tgtatctatt tgtttattta accctaattt agaacctgca agctcttttt caagagattg
     8281 agcagtggca aggttctttc ttaaggctcc tttttggtcg ctaatccctg aatgcgtttt
     8341 tttggcaccg atacccgtta tacctacttg tgtgactgct ccagtaatgc ttgatgatag
     8401 ggctgccagg ccttgccgaa caatgttctc tgcagctgat tttgtagcat cgaacgcaat
     8461 caatgacaat tgagagccca atttagtttc tgcagtgcgg agagcagaaa gtagaacggc
     8521 tacactaata attaaagaaa cagcattaga agaaagactg gaaatatcca gagtgttctc
     8581 tggagtaagg gtgtgactca tctctgaaag atctttcatc tttttgtcag taaggcgagc
     8641 aatgatttct gaatcatgct gaacctgctc tgataacttt aaaagttgat catcatttaa
     8701 tgtggttgtt aatacgggat ttttaccgac attaagtgga atatgcgctg caatctgctg
     8761 ataattttgt gatttttgtg tttcggaaga actttgagtt tgttttgtgg atatatctgt
     8821 atataaaatc tgggttggtt ttgtgttttg aatttccata acattctcct tatttgtatc
     8881 aagcagtagt ttgttgcaaa attgcttttg caacatcagt tctattagcc tgagagttgg
     8941 acattgaggc taatagatct gcaattactt cctgcaattg gccgaatttt tctattgctt
     9001 cactgatata ttttgacagt tgttcaactt gatatttcga taatgtcagg tctgctagat
     9061 ttgtcgacgc gctgttctgg aaaacagcag aagcgacact tcctcccgct tgtgtggcag
     9121 agttaacact ttctcctaat aaaactgctt ggtttaaatg ggtggaaata atttgtttgg
     9181 atattacttc atcacccgct gcaccaagaa atttatttaa tctggcaaca gcattagtga
     9241 ttaaatcgtc cagttgagaa gaaaaattct tgagaaactt tggtataagg tctgtgaggg
     9301 ttttacctat tattttgcca atattttctg caagttttgc tgctgcctgt ttaccaacag
     9361 tggctacgag aacgactgct gcaactagga caagagcgcc tgcgattgcc cccagaatag
     9421 agccaatcat tttggctttt ttcgagtcga cgcccaagcc ttcgagcatt tttgtaaatg
     9481 catctgaaag gagtttgatt aagggttcaa tgactgcttt catgatcgga ttcagggctt
     9541 gttccatgaa ggaattgccg gtcgctgctt gtactatagc atccgtaacc ataagagcta
     9601 aaccaacagc tgccagtgct agagaggctc ctccagaaaa agctgctgca acaacactaa
     9661 cgatagttaa aagtgcccca agtattttcc caacacaacc cattactctg ttgagttctt
     9721 ctgctttacg tacttcagca gcatactcat cagattttct ctccatttca gtttttcttg
     9781 attcttggag agactggaat agagccagat cattttttaa agattcttca ttattttttc
     9841 caactagttg aataaaggtt gccatcaatt gagtaacact ggcaagtccg gttaatgatt
     9901 tctgctgggt tgatagctgt tcagcagatg ctgtgtttga aaatgcagaa aaagagtcta
     9961 tttctttttc gagttgcatt gatttatctg taagtttgct atgaattgac agggttttct
    10021 gctcaatcaa tgtcctgtct ttaactgctg cgtctttttt gatagtgagt tgtatttctt
    10081 cccggcttaa ttttttcttt tctggtgact ctgggtcgag ttcggataat cttgtttgaa
    10141 tttggttaat tttattttct aggtctttta ttttagaatc tgcgtttttt agtttattaa
    10201 tttgtttttc atagtctctg gttagtcctt cagtttcaga tagaagagtg ttaattttat
    10261 cggagaattc taggtttttt tgctgtcttg cctgttgctg ggacttccaa gcagtaattt
    10321 tatttgttaa tgcagttaaa gatttttcac cgagtatttg aataaggttt ccaattaaaa
    10381 gcgttagttg ggaacttgca tttaatgact ttggtgcttt aagttcaggg attaatatat
    10441 ttgaagttga gtgtgcatta gttgtattaa tattttggtt agcagtaaga tcagcaattg
    10501 taagagaaaa taatttgtta gctgcatcat ttgcagcttg gatagtattg tctccaagct
    10561 cagtggaagc caatattttg gcaagaggaa aaccagtggt tgtggtgctt acattatgca
    10621 taataattac tccttgatat cctgaattgc gtccaagtat gattgtgctt ttatttttaa
    10681 tttttcatca ttgctgtgtt gaattacgag ttcgaagcac tctttagctt ttaagggggc
    10741 tttcaaccga agctggcatt gtccagtatg gaatactggt gtatagtcat tttttcctaa
    10801 tgcaaaagcg acagcataaa ggtctgctgc ttgttggaac tgttctttta tctgataaat
    10861 agctgcgagt cccataatgt agtctacatt gtaaaagtcg tatatacata aaaacctgaa
    10921 gaaaacttca gcttcctcta ttcttccttt gttgtaaaag tcataagcat atgaataaat
    10981 gtcatccatc atatcatcag gaattgcatt aatatctttc agtgtagcgc cagagttaat
    11041 tgcatcaatt actgcagtag agatgctttc attttcggtg atatttaaag acataaggtc
    11101 tcctttttgt ctgctttact ttaataagta taagatttaa tataaaagat ttaatttgta
    11161 ttgctttgac ggtatacagc ctttagaacc tctgcgctta tttctatttg taaaactttt
    11221 gttgattcat taaatatttt atcgtagacg gaactggata ctagatttgt aagatccttc
    11281 atttgtgtgt taaacaaatc catgtcactg ctatagcgtt ttaaaatctt tgctctgagg
    11341 ccagatgagg caatctcgtt tagtgctgta tgactgatag atgtttgtgc tgatttttca
    11401 tcaagtttta tatccgggaa ttttttttca atagctgcaa atatgttttt tttgttatct
    11461 gaggtcaaaa caactccatt gtgtatggcc atttcatcta tacttctgtt tatcattgaa
    11521 gagacataag tggttcgctc ttggctcatc caacaaaata gttgattccc cgaattacta
    11581 tcttttattt tgtctatgac gctgtccctg attttattta tattcaccgg gcatccaaaa
    11641 ttcttctgag gaaagcgttg ttttaagtta aaaacagata atattttctt tacaagctta
    11701 ttgcgactta acgaatcaaa tgatgggcga ggaatcgcga attcaacctg tggaagtatt
    11761 ttgtttagaa tttgcatgat accccctata tgttagttca cttctgaagt gatgtttgca
    11821 atggttagtt taatacaaaa taaaaattca tacaaagatt caatgagttc atcaggtgaa
    11881 agatattttt tgtagtgttt cttggtcaaa acacattgat gagtatcagg gagaagacag
    11941 atagaagaac cataatctct actcaaagag tagttaagga tgagtatgtg ttctggatta
    12001 ataatatttt ctgtaaaaag ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca
    12061 tcattatctg ttatacaaac aggtatttgt tcttcgagca atataaaggc ttgattttta
    12121 tcgttaaaat caagctttgc acctgttatt tcataaagtt tatcatatag tttgcgacac
    12181 atttctatgg ccttctatat attagaatga gagttattca ataaaagctt attgatctgt
    12241 aataagtcgt gaatgtaaag tttggttcca agatctggcg atttaagaga attgtaataa
    12301 tcaataattt ttattgattt ttcgatgatt tcttcttttt tatctgttgc ttcggttttt
    12361 aattcgttta atacacgagt catattgctg ggtgctgatt ttactaagta tgagtggcaa
    12421 gcgttgatga ccgcatcaaa gtcttctgaa gatacatttt cagtaggatg tttgtaaagt
    12481 gaaattgata aaggcttgaa tgtttttggt gtgtggataa ggttaatttt attgaaactt
    12541 ttaagatagc tgcgtcgtaa ttgagtcaat ctggaaagat caaaatcaga ttcaatatac
    12601 ttcattattt cgttataaga ctgattgaga ctttcaatgc gttgcctaac tttatttgca
    12661 tacgcgtata catcgttagg tgtagcaacc aatttaacgt cagggagtgg aaagtatatt
    12721 tcagaatttc ctgctttaag aacattaagt gccataccag cacagttttc agatttactg
    12781 attaatttat aatttgccaa ttgtctgaaa gcatctgctt tctttttgca tataaatttt
    12841 gacatgtttg tttcatcaag gccaaaaagg ttatggctga ttttaccatc ctttgtttta
    12901 ttatcacctg ataatggaat gtatacttta tcagcactga ccccccaata accatagggg
    12961 tctttttttt gattagctct gggaaaaaat atttttttgt tgttattttg atcttgtttt
    13021 gaatttaata agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa
    13081 atcatattta gtttgtcttg cttataggta tcgacactgt aacctgagcg tttttcaaaa
    13141 aggtagttta tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta
    13201 tgagttggtt cctgtagtaa attgtttttt atactgatac tagtatgccc taaatgggtt
    13261 atgcctctat ttccccagca acgaaaaact aaatgtcttt caagattttt ggctgtaccg
    13321 atgccatgaa aacgctctgc ggtattcaac attaatttga tgtctctacc tgtgaaagag
    13381 tgttgaattg agtttttagc tgcttcatag gcggcaaacg cactttctgc agatttttcg
    13441 ctagctaatg tattgaggaa aagcaataag gatttttgat tctctagtcg ataatttttt
    13501 agttgttcat ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa
    13561 aagagatcct ttaggttgaa tttctgtgct atcaatatag gtccatgctc tgtatcttca
    13621 acgcgtatag gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gaggatcata
    13681 ctttattaac tctccattac ttggtgattg tggggatgta aaatgtattt ggtatataat
    13741 atattcaatt aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgct
    13801 gtacataata tgtacctcgt gagcatatgt agtgctcgtt tcatcatgaa atcccacaag
    13861 ataaagtgcc tgatgtatca ggctcggagt gttatagaaa aagagagaac cctgttgaat
    13921 aagtatcttt tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgact
    13981 gaggataatt aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga
    14041 gattcctata aaggcgcaga agaaaccgga aagcacaaag gtgtaagcgt gatttcatat
    14101 caaagagtta aaaatggaga aagaaataag gggatcgaag cgcttaatcg attatattta
    14161 cagaaccaaa caagcttaac aggaaaaagc cttttgtttg cgcgtgacag agctgaagtt
    14221 ttttgcgagg caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa
    14281 cgattagata cctataaact tggtgaagtt aataaaagac atattaatga gcttaataaa
    14341 gtaataagtg aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaaacg
    14401 aaaataaaac aaatatttac tgattattta aataataaga attggggacc ggtcgacaag
    14461 aatattagtc atcatgggaa aaattatggt tttcaattaa ctcctgcctc tcatatgaag
    14521 atagggaata aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct
    14581 acgagagaga gtgatcatat cgcgaatatg tggctttcaa aagtggtgga tgatgagggg
    14641 aaggaaattt tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac
    14701 tcatctgaaa gagctgttgc tgctcgtaat aaagctgagg agttagtaag cgcagcatta
    14761 tatagcagac cagagttatt atcacaggct ttgtctggta aaacagtaga tttaaagatt
    14821 gtttcgactt ctcttctgac gccgaccagt ttaaccgggg gagaggagag tatgttaaaa
    14881 gatcaggtga acgcattaaa agggttaaac tctaaaaggg ggaagccaac gaaattatta
    14941 attcggaata gtgatggtct tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt
    15001 aactttggtg taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag
    15061 cttaatgatg aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt
    15121 ggaggttggg ctgcagaagc aatagaaaaa aatccaccat gtaagaatga tgtgatatat
    15181 ctggctaacc agataaaaga gattgtaacc aaaaagttac aaaaaaatga taatggagag
    15241 ccgtataaat tgtcacaaag aatgactcta ttggcttata ctataggtgc tgtaccttgc
    15301 tggaattgta agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa
    15361 ataataagaa aacatgaaac aggtcagttt tctcaattaa atagtaaatt atcctcagaa
    15421 gagaaaagat tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg
    15481 aatactggtg tgcccggaaa taaagtcatg aaaaaattgc cgctatcttc gttagagcta
    15541 tcttattctg aaagaatagg ggacccaaaa atatggaata tggtgaaagg gtattcgtca
    15601 tttgtataag aggaatatat ggaagattta gcagatgtta tttgccgagc cttgggtatc
    15661 cctttaattg atatcgatga tcaagcaatc atgcttgatg atgatgtgct aatatatatt
    15721 gaaaaagaag gagattcaat aaatttatta tgcccttttt gtgcattgcc tgaaaatata
    15781 aatgatctta tatatgcgtt gagcctaaat tactcagaaa agatatgctt agctactgat
    15841 gatgaaggcg gaaatctaat tgcgcgatta gatcttaccg ggataaatga attcgaggat
    15901 gtatatgtta atacagaata ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg
    15961 cgaagaatga aggggtatta atgtcccgtt tcgtatttat tttgctgtgt tttatcccat
    16021 atttagggag agctgattgt tgggataagg ctggtgaaag gtacaatatt ccatcaagtt
    16081 tgttaaaagc gattgcggaa aaagaatccg gatttaataa atctgcagtc aatgttaata
    16141 acaatggaag taaagattat ggtataatgc aaataaatga ctttcattct aaaagactta
    16201 gagaaatggg atattctgag gaaatgctaa ttagtcatcc atgtctttct gtacattatg
    16261 cagcaaagtt attaaatgag tttatgatga tgtatggaag agggtgggag gctgttggag
    16321 cctataatgc aggaacgtct ccgaaaaaga aaaaagaaag attaaagtac gcagaggata
    16381 tctatagacg ctatttgcgg atagcagcag agagcaagca gaataatcga aggatataag
    16441 aggatttatg tctgaggcaa agaactcaaa tcttgcacca ttcaggttat tagtgaaact
    16501 cacaaacggt gtcggggatg agtttccgtt atattatggg aataacttaa ttgttctcgg
    16561 acggacgata gaaacacttg agtttggaaa cgacaatttc ccagaaaata ttattcctgt
    16621 aacagatagt aaatcggatg gtataattta tttgaccata agcaaagata atatctgtca
    16681 atttagtgat gaaaaaggtg agcaaattga tatcaattca caatttaatt catttgaata
    16741 tgacgggatt tcttttcacc ttaaaaatat gagggaggat aaatcgagag gccatatact
    16801 aaacggaatg tataagaacc actcggtgtt ttttttcttt gctgttattg ttgtgttaat
    16861 tataattttt ttcctctctt taaagaaaga tgaagtaaaa gaaattgctg aaattataga
    16921 tgataagaga tatggaatag tcaacactgg acagtgcaat tatattcttg ccgaaacaca
    16981 aaatgatgcg gtatgggcgt ctgtagcctt aaataaaacc ggatttacca aatgcagata
    17041 tattctggtg tcaaacaaag aaataaatag aattcaacaa tatatcaatc aacgattccc
    17101 atttatcaat ttgtatgtgt tgaatttagt gagtgataaa gcggaattac tggtgttttt
    17161 aagtaaagaa agaaatagtt caaaagatac cgaacttgat aaattgaaaa atgctctgat
    17221 tgttgaattt ccgtatatca aaaatattaa attcaactat cttagcgatc ataatgcaag
    17281 gggggatgct aaaggtattt tcacaaaggt aaatgtccag tataaagaga tttgtgaaaa
    17341 taataaagtt acatattctg tcagagaaga actgacagat gaaaagcttg agctcattaa
    17401 tagactgatt tctgaacata aaaatatata tggagatcag tatattgagt tttctgtact
    17461 tttgatagat gatgatttta aaggtaaatc atatcttaac agcaaagaca gttatgtaat
    17521 gttgaatgat aaacactggt tttttttaga taaaaataag tgaggataaa atgagtgtta
    17581 cagtaccgaa tgatgattgg acattgagtt cattatctga aacttttgat gatggaactc
    17641 aaacattaca aggtgaacta acattggcac tagataaatt agctaaaaat ccttcgaatc
    17701 cacagttgct ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc
    17761 aatccaatac agtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat
    17821 aatagggagc attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca
    17881 gtgattttca atctcaagag atatcaagtc tggaagacgt cgtgtcggct aaatatagtg
    17941 atattaagat ggatacagat attcaagtat cacaaataat ggagatggta agcaatccag
    18001 aatcattaaa cccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag
    18061 gagtatcatt agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa
    18121 agtcttaatt tatatgatta ggtataaagg ttttatttta ttcttgttgc tgatgttgat
    18181 tggatgtgag caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat
    18241 aatatctgtg ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg
    18301 gatctcggta caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta
    18361 cgatttgcca aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt
    18421 gtcttctcca agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca
    18481 gtctttagtt tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga
    18541 agaaaaaaat atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc
    18601 gaaagagtct gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga
    18661 tgttaagtat gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa
    18721 tgtacaacct gttaaggaag ttaaatcgga atttttaaca aatgaagtaa tatatttatt
    18781 tctcgggatg gctgtactag ttgtcattct tttggtatgg gcattcaaaa cagggtggtt
    18841 caagagaaac aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt
    18901 tttgatccag cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa
    18961 aatggcgtaa tcagaagtga gattaataat ctcataatta ataaatatga tctaaattgc
    19021 gatattgaac ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca
    19081 gctgttgctt attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt
    19141 attagttatt atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt
    19201 tggcatctta ttgcgtggaa ggaaaatata tctgttggta tatatgaaag aataaactta
    19261 ttatttaatc caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtcccga
    19321 ttaaatgaag gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta
    19381 tgatggtgtt gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga
    19441 cactgagagt aagaagaaag caatttgtgt tataaaggag gctacgaaaa aagctgaatc
    19501 actgcgtata gatgctgtat gtgatggtta tcaaattggt atacagactg cttttgagca
    19561 tattattgac tatatttgtg aatggaagtt aaagcaaaat gaaaatagaa gaaatattga
    19621 ggactatata actagtttgc tttcagaaaa tttacatgat gagaggatca ttagcacttt
    19681 attggagcag tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc
    19741 caagtgtaat ttagcactga ggaaaaagtt agaacttgat ctccataaat atcgctcaga
    19801 tgtcaagatt attttaaaat attctgaagg gaataattat atattctgta gtggtaatca
    19861 agtagtggag tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa
    19921 attaacaaaa aatgataaaa aatatttcaa agagttagca cataagaaac tgagacaaat
    19981 tgcagaagac ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg
    20041 ctctgcagca aagattaaat agtgaagaag ttgttaattt aaatgagaga ttatcatctt
    20101 cgcaatcatt tgatgaggat ataatctatg aaataatgca atatttctca cagagtgaat
    20161 taaattccat agataatgat gaactgcaca ataagattga acaattattc aattcacgtt
    20221 ttccttatct tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc
    20281 aatatgtcga tttgcatgag ggattttatg catcattgtc catatataac aatattgatt
    20341 tctatattaa gactacaacc ttcgattcat taattagtgt ctttgaagca gggagagagg
    20401 cagatgattc gacatggtag taataagttg aaaatattta ttttaagtat attgctatta
    20461 acactgagtg ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt
    20521 cctgtaagta aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc
    20581 aataaaagta taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat
    20641 gcaggtaaat tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca
    20701 caaaagatag ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt
    20761 aataagataa atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt
    20821 cgagggatat aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta
    20881 catttactct agtggacatg aaccagttaa cgttgagctt gtaaaagata aagaacgtaa
    20941 cataattgag ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa
    21001 ttcagtgata atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt
    21061 gctttttggg actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt
    21121 catttctatc atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag
    21181 ttaatgttaa gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata
    21241 cgaatgactg agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag
    21301 gaggtaagaa taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct
    21361 ttatgtagaa aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta
    21421 aatggtttat tagatgtatt tttgcacaat caaacaatca cttcagctgc catgaataat
    21481 gggtatgcat caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca
    21541 agagaattat cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa
    21601 atctttgact ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc
    21661 tcatttatta gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc
    21721 tttttttgaa cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc
    21781 aaaaaagaga atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaact
    21841 gacattactt gtgggactca tatggtataa agatggaaat gcattataca tatatgactc
    21901 tggagaatta ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca
    21961 gtatctgaag gatgctaacc tttatgatca cagatatcct ataagaggaa acattagtga
    22021 taaaacattc tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac
    22081 tttgcttgat aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa
    22141 attaaaaaat acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat
    22201 cccaggggtg gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag
    22261 acaagcccaa aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag
    22321 taatgacttt tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat
    22381 tgcatatcca gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat
    22441 tcgcgatatt atcactcagt tagatatagc caaacgacat atagaattat cattatggat
    22501 aatcgatatt gataaatctg aattaaataa ccttggtgtt aattggcaag gaacggcttc
    22561 atttggtgat tcgtttggtg cgtcatttaa tatgagctca tcagcatcga taagcacttt
    22621 ggacggcaat aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt
    22681 agtttcaagg ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag
    22741 gactttttat gtgtcattag ttggtgagcg aaattcttcg ttggagcatg ttacgtatgg
    22801 aacattgata aatgttatac ctcgcttttc atctcgggga caaattgaga tgtctttaac
    22861 gatagaggat ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc
    22921 tgtccttcca gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa
    22981 aagtttgttg ataggtggtt atacgcatga aacgaactct aacgaaataa tatctattcc
    23041 ttttctctcc tctattcctg taatcggtaa tgtgttcaag tataaaacaa gtaatatcag
    23101 taatattgta agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa
    23161 tactgcagaa tataaaagcc tcatatctga aagagaaata caaaagacta cacaaataat
    23221 cccatctgaa actactttgc tggaagacga aaaatcattg gtttcatact taaattacta
    23281 actataaagt aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt
    23341 cattgataag ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga
    23401 gcttgattct ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc
    23461 tctttcgtca tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag
    23521 tcaggaacgt attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa
    23581 gagaacgtta aaagataacc ttcctctaga tcgggatttt atagacagac taaagagata
    23641 ttttaaagat ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct
    23701 tactgctgaa caagtcgaat tattaactaa aattattaat gagataatat caggtagtga
    23761 aaaaagtgtt aacgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat
    23821 gaaacttgaa ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc
    23881 aacaacagat cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat
    23941 tgtgaatttt gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa
    24001 tgcttatgag tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc
    24061 agacgtaatt tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag
    24121 tgcagagcag ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca
    24181 aattcttact tctgttatag aagtatcacg agccagtcat gaggattctg tagtgtatca
    24241 aacatatcta tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag
    24301 agaaattgcg atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc
    24361 tagataacag gagataaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga
    24421 attaattata ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac
    24481 ataccttgtt gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg
    24541 atcattttat attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac
    24601 gacattattt cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga
    24661 tgcgggtaaa attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg
    24721 ttttgttatt ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga
    24781 aagggttgcg gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag
    24841 cattgatgcg gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag
    24901 tattctggaa cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa
    24961 aggtgatgca attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt
    25021 tggtatgagt caacatggaa tgtccctatc tggtgcatta tctacttata ccatactaac
    25081 tattggtgac ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt
    25141 tatcgttaca agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat
    25201 atttgggaat ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt
    25261 gccaggtttt ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta
    25321 ttataaaaag gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac
    25381 tggtacattt gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga
    25441 tgctattagt tcagaaaccg ttcctttaat tttattattt gccgaaaata agataaatgc
    25501 aaatgatatg gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt
    25561 caggcttcca actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt
    25621 gttaataaat gaggtgcgtg ccgatagttt taatatatat tttgacaaag tgtgtattac
    25681 agatgaaaat ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga
    25741 acgtgttatt tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa
    25801 aattaaaagt gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat
    25861 aaatgagatt tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta
    25921 tcctgatcta ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt
    25981 acaaagattg cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt
    26041 ggcgctttgg gctcccagag aaaaagatgt cataacatta gttgaacatg tccgtgcatc
    26101 actttctagg tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct
    26161 ttccggatat attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt
    26221 cttgaatatg gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag
    26281 agaattgaga aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt
    26341 tgttaaaaga cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat
    26401 tgatgaagca tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa
    26461 cattaattta gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat
    26521 tattacagat ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat
    26581 taatatagct cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga
    26641 tgtaaaactt cagtcatctg cttataacat attaaattta atgttaatga actttagtta
    26701 tagcattaat gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt
    26761 tattaaagat gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta
    26821 tcagaggatg gaaatcttaa atggggtctt ataatcaatg agctatacaa aattgctcac
    26881 tcaattatct tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt
    26941 ttcgattggt gagatttgta acattcaggc tggaattgaa agtaatgaaa ttgttgcaag
    27001 agctcaggtt gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg
    27061 tggactttca cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg
    27121 ccgtggatta ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt
    27181 tacagataac agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga
    27241 aagggctgca attgagaagc cttttttaac aggtattaag gttattgatt ctttactcac
    27301 gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct
    27361 catgaatatg ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga
    27421 gcgaggtcga gaggttactg aaacggttga ttatttgaaa aactctgaga aaaaaagcag
    27481 gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata
    27541 tatagccact gctatagccg aattttttag gactgaagga cataaagtag cgctttttat
    27601 tgattcatta acaaggtatg ccagagcatt acgtgatgtg gccttagccg ctggagaatc
    27661 acctgccaga agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag
    27721 gccaggaaag ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga
    27781 tgatgatttt gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata
    27841 tttgagcaga aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat
    27901 aagcagggta tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg
    27961 ggagctactt tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc
    28021 gggggagaat gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct
    28081 gaagcaagat tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac
    28141 aattagataa ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag
    28201 cacacatatt aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa
    28261 aggattatat acttcctgca actggagaat gacttagaac ctgtaggcgc tcagagtgtt
    28321 agtcagcttt tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat
    28381 cagtctgaac gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag
    28441 gcaaatgctt ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc
    28501 ttgattaaaa gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat
    28561 atgaagtatg gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa
    28621 atgtgagaaa ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa
    28681 ggttgaaatt cctgagtact ctttaattgc atctaactat tttactattg ataagcactt
    28741 tgaacataag catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag
    28801 aaacgaacga gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt
    28861 gataccagat caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat
    28921 cttttcatat aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc
    28981 tgtaaaccct gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa
    29041 tcatagcttg tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt
    29101 tgctaaatca tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat
    29161 gtcattgcaa gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga
    29221 aatgacttac cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc
    29281 tggttctttt gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt
    29341 gaaacaagat gctgagggta attacagatt tgatagcagt caacataata aggggaataa
    29401 aaataatagt acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt
    29461 gacgctaacg aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt
    29521 cagacattca aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt
    29581 ggggattggg taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt
    29641 tcaggatttt ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg
    29701 tcatctgtat tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa
    29761 atccagtatg caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt
    29821 tattttgaca agatgccggt attacgtaat caagtttctc ttgactcatt gcatcatctt
    29881 ttagagtttt gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgcact
    29941 ggtgatataa tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt
    30001 ggggattata ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt
    30061 gagtcagaac acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa
    30121 gtggacttta ttcttttaga aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa
    30181 aacgaattat tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt
    30241 tctttggttg ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt
    30301 tcttggatgg taaaggagta atctcatgct gagtgacatg tccctcatcg ctacactttc
    30361 tttttttacg ctattaccat ttttagttgc tgctggtacc tgttatataa agttttctat
    30421 tgtttttgta atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct
    30481 caatggtatt gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta
    30541 tgaaaattat ctaaatggcc ctcaaaaatt tgatacaata tctgatattg tccgtttttc
    30601 tgattctggc ttgatggaat ataagcaata cttgaaaaag catacagatc ttgagttagc
    30661 acgttttttc caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga
    30721 ttattcattg ttttccctct tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa
    30781 aataggtttt tatttatatt tgccatttgt tgttgttgat cttgttattt caagcatttt
    30841 acttgcactt ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt
    30901 attatttgtt gcgcttgatg ggtggggtat cctgtctaag gccttgattg aacaatatat
    30961 caatattcct gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag
    31021 gctctttatt taatccttat cttttcctta tggccagtag gtatagctac ggttattgga
    31081 ttatctattg gtttgttaca gacagtgact caactccaag agcagacact tccttttggt
    31141 ataaagctta taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt
    31201 ttattgtctt tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt
    31261 caagctggtt cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat
    31321 tggctccatt atttttcttt cttccatttt taaataacgg tataatttct ccatctatta
    31381 gaatacctgt gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag
    31441 gttcttctgt ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc
    31501 ttctctcttt ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg
    31561 acaaccagcg tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt
    31621 ctgagttggc aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggta
    31681 tggtctttat tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat
    31741 gttctttccg tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta
    31801 ttttagccag tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat
    31861 cgagatttgc tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg
    31921 caatatttat tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc
    31981 tcggtgaaca taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa
    32041 cagaaaagcc gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat
    32101 ttaaggattt aacgactgtt gttattattc tggtagggac atttactata atatcattct
    32161 tttccttaag tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta
    32221 atgagggtaa atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac
    32281 tttttttctg tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg
    32341 cgactaaagc tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa
    32401 tatttagtat aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag
    32461 cattaacaac ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt
    32521 ctagtgttga tggcttatat cttatttggg ggaggctgtt taaggatata atattatttt
    32581 tcttggcatt ttctattctt gttattattc ttgactttgt gattgagttc attttataca
    32641 tgaaagatat gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac
    32701 actttgagac aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta
    32761 aatctgatat acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta
    32821 tttattttaa tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt
    32881 gtgccttggc tgtcagaaaa tatgcaaatg aagttggtat accgactgtg cgtgatgtga
    32941 aattagctag aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact
    33001 tggatgaagt cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag
    33061 taaaggagat gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg
    33121 aatttgaaat agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat
    33181 ctttccttag caataaggat ataataaatg ttttactcag aaagatttca tctgaatgtg
    33241 acctggttag aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg
    33301 atgatttata atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat
    33361 ttatgtagtc acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa
    33421 ataatatatg ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga
    33481 taagagagac tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc
    33541 aaagtctgta aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat
    33601 ttcagatagt aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg
    33661 taaaaggaga atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg
    33721 taaactatat tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg
    33781 tttttgatat gtttactcca acaacggaga caggtaaatt atggacgaaa agaagcttaa
    33841 agcccttgcg gctgaactgg ctaaaggcct caaaactgag gccgacctca atcagttttc
    33901 ccgtatgctg acgaaatatc ccggctctta aaacgatagt ggctcaggct ttcatttttc
    33961 agcgttcccc agaag
//
LOCUS       DADLEE010000042        33525 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000042 DADLEE010000000
VERSION     DADLEE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33525
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.042"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="JQH51_002057"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="JQH51_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY6076400.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="JQH51_002058"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="JQH51_002058"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY6076401.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQH51_002059"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQH51_002059"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY6076402.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQH51_002060"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQH51_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY6076403.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQH51_002061"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQH51_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY6076404.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /locus_tag="JQH51_002062"
     CDS             complement(3426..4094)
                     /locus_tag="JQH51_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY6076405.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="JQH51_002063"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="JQH51_002063"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY6076406.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="JQH51_002064"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="JQH51_002064"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY6076407.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="JQH51_002065"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="JQH51_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY6076408.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQH51_002066"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQH51_002066"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY6076409.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQH51_002067"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQH51_002067"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY6076410.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="JQH51_002068"
     CDS             8212..9075
                     /locus_tag="JQH51_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY6076411.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="JQH51_002069"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="JQH51_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY6076412.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="JQH51_002070"
     CDS             10411..11028
                     /locus_tag="JQH51_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY6076413.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQH51_002071"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQH51_002071"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY6076414.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="JQH51_002072"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="JQH51_002072"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY6076415.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQH51_002073"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQH51_002073"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY6076416.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="JQH51_002074"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="JQH51_002074"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY6076417.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="JQH51_002075"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="JQH51_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY6076418.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="JQH51_002076"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="JQH51_002076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY6076419.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQH51_002077"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQH51_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY6076420.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="JQH51_002078"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="JQH51_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY6076421.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="JQH51_002079"
     CDS             21859..23568
                     /locus_tag="JQH51_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY6076422.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQH51_002080"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQH51_002080"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY6076423.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="JQH51_002081"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="JQH51_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="JQH51_002082"
     tRNA            27625..27719
                     /locus_tag="JQH51_002082"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="JQH51_002083"
     CDS             27991..29202
                     /locus_tag="JQH51_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6076424.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="JQH51_002084"
     CDS             29614..30657
                     /locus_tag="JQH51_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY6076425.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31633
                     /locus_tag="JQH51_002085"
     CDS             31172..31633
                     /locus_tag="JQH51_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076426.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(31630..32485)
                     /locus_tag="JQH51_002086"
                     /pseudo
     CDS             complement(31630..32485)
                     /locus_tag="JQH51_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32482..32781)
                     /locus_tag="JQH51_002087"
     CDS             complement(32482..32781)
                     /locus_tag="JQH51_002087"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6076427.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            33237..33494
                     /locus_tag="JQH51_002088"
     CDS             33237..33494
                     /locus_tag="JQH51_002088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076428.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8401 a         8810 c         8260 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttga
//
LOCUS       DADLEE010000043        31185 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000043 DADLEE010000000
VERSION     DADLEE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.043"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            389..2047
                     /locus_tag="JQH51_002089"
     CDS             389..2047
                     /locus_tag="JQH51_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY6076429.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="JQH51_002090"
     CDS             complement(2044..3000)
                     /locus_tag="JQH51_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY6076430.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="JQH51_002091"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="JQH51_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY6076431.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="JQH51_002092"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="JQH51_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY6076432.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQH51_002093"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQH51_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY6076433.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="JQH51_002094"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="JQH51_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY6076434.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="JQH51_002095"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="JQH51_002095"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY6076435.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="JQH51_002096"
     tRNA            complement(9859..9946)
                     /locus_tag="JQH51_002096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JQH51_002097"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JQH51_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY6076436.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JQH51_002098"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JQH51_002098"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY6076437.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JQH51_002099"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JQH51_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY6076438.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JQH51_002100"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JQH51_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY6076439.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JQH51_002101"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JQH51_002101"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY6076440.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="JQH51_002102"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="JQH51_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY6076441.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="JQH51_002103"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="JQH51_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY6076442.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="JQH51_002104"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="JQH51_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY6076443.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /locus_tag="JQH51_002105"
     CDS             22003..23346
                     /locus_tag="JQH51_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY6076444.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="JQH51_002106"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="JQH51_002106"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY6076445.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="JQH51_002107"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="JQH51_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="JQH51_002108"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="JQH51_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY6076446.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="JQH51_002109"
     CDS             28042..28833
                     /locus_tag="JQH51_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY6076447.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /locus_tag="JQH51_002110"
     CDS             complement(28869..29495)
                     /locus_tag="JQH51_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6076448.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="JQH51_002111"
     CDS             29810..30958
                     /locus_tag="JQH51_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076449.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7645 c         8373 g         7478 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcag
//
LOCUS       DADLEE010000044        30296 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000044 DADLEE010000000
VERSION     DADLEE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.044"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JQH51_002112"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="JQH51_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY6076450.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JQH51_002113"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="JQH51_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY6076451.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JQH51_002114"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="JQH51_002114"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY6076452.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JQH51_002115"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="JQH51_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JQH51_002116"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="JQH51_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY6076453.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JQH51_002117"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="JQH51_002117"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY6076454.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /locus_tag="JQH51_002118"
     CDS             complement(6041..7180)
                     /locus_tag="JQH51_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY6076455.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7839..9422
                     /locus_tag="JQH51_002119"
     CDS             7839..9422
                     /locus_tag="JQH51_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY6076456.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JQH51_002120"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="JQH51_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY6076457.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JQH51_002121"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="JQH51_002121"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY6076458.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JQH51_002122"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="JQH51_002122"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY6076459.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="JQH51_002123"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="JQH51_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="JQH51_002124"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="JQH51_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="JQH51_002125"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="JQH51_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="JQH51_002126"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="JQH51_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY6076460.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="JQH51_002127"
     CDS             20752..22167
                     /locus_tag="JQH51_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076461.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="JQH51_002128"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="JQH51_002128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY6076462.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="JQH51_002129"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="JQH51_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY6076463.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="JQH51_002130"
     CDS             24477..24809
                     /locus_tag="JQH51_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY6076464.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /locus_tag="JQH51_002131"
     CDS             24956..26317
                     /locus_tag="JQH51_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY6076465.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="JQH51_002132"
     CDS             complement(26659..26976)
                     /locus_tag="JQH51_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076466.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="JQH51_002133"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="JQH51_002133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY6076467.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29142)
                     /locus_tag="JQH51_002134"
     CDS             complement(28363..29142)
                     /locus_tag="JQH51_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6076468.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="JQH51_002135"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="JQH51_002135"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY6076469.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7010 a         8049 c         7828 g         7409 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 acaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagcta
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgatt
//
LOCUS       DADLEE010000045        30025 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000045 DADLEE010000000
VERSION     DADLEE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30025
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.045"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            261..644
                     /gene="secE"
                     /locus_tag="JQH51_002136"
     CDS             261..644
                     /gene="secE"
                     /locus_tag="JQH51_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY6076470.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            646..1191
                     /gene="nusG"
                     /locus_tag="JQH51_002137"
     CDS             646..1191
                     /gene="nusG"
                     /locus_tag="JQH51_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY6076471.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1350..1778
                     /gene="rplK"
                     /locus_tag="JQH51_002138"
     CDS             1350..1778
                     /gene="rplK"
                     /locus_tag="JQH51_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY6076472.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1782..2486
                     /gene="rplA"
                     /locus_tag="JQH51_002139"
     CDS             1782..2486
                     /gene="rplA"
                     /locus_tag="JQH51_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY6076473.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2899..3396
                     /gene="rplJ"
                     /locus_tag="JQH51_002140"
     CDS             2899..3396
                     /gene="rplJ"
                     /locus_tag="JQH51_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY6076474.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3463..3828
                     /gene="rplL"
                     /locus_tag="JQH51_002141"
     CDS             3463..3828
                     /gene="rplL"
                     /locus_tag="JQH51_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY6076475.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4148..8176
                     /gene="rpoB"
                     /locus_tag="JQH51_002142"
     CDS             4148..8176
                     /gene="rpoB"
                     /locus_tag="JQH51_002142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY6076476.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8253..12476
                     /gene="rpoC"
                     /locus_tag="JQH51_002143"
     CDS             8253..12476
                     /gene="rpoC"
                     /locus_tag="JQH51_002143"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY6076477.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12718..13851)
                     /gene="thiH"
                     /locus_tag="JQH51_002144"
     CDS             complement(12718..13851)
                     /gene="thiH"
                     /locus_tag="JQH51_002144"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY6076478.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13848..14693)
                     /gene="thiG"
                     /locus_tag="JQH51_002145"
     CDS             complement(13848..14693)
                     /gene="thiG"
                     /locus_tag="JQH51_002145"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY6076479.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHATQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEVGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14620..14820)
                     /gene="thiS"
                     /locus_tag="JQH51_002146"
     CDS             complement(14620..14820)
                     /gene="thiS"
                     /locus_tag="JQH51_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY6076480.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14804..15559)
                     /gene="thiF"
                     /locus_tag="JQH51_002147"
     CDS             complement(14804..15559)
                     /gene="thiF"
                     /locus_tag="JQH51_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY6076481.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15552..16187)
                     /gene="thiE"
                     /locus_tag="JQH51_002148"
     CDS             complement(15552..16187)
                     /gene="thiE"
                     /locus_tag="JQH51_002148"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY6076482.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16187..18082)
                     /gene="thiC"
                     /locus_tag="JQH51_002149"
     CDS             complement(16187..18082)
                     /gene="thiC"
                     /locus_tag="JQH51_002149"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY6076483.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18163..18263)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18316..18792)
                     /gene="rsd"
                     /locus_tag="JQH51_002150"
     CDS             complement(18316..18792)
                     /gene="rsd"
                     /locus_tag="JQH51_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY6076484.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18887..19660
                     /gene="nudC"
                     /locus_tag="JQH51_002151"
     CDS             18887..19660
                     /gene="nudC"
                     /locus_tag="JQH51_002151"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY6076485.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19700..20764
                     /gene="hemE"
                     /locus_tag="JQH51_002152"
     CDS             19700..20764
                     /gene="hemE"
                     /locus_tag="JQH51_002152"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY6076486.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20921..21592
                     /gene="nfi"
                     /locus_tag="JQH51_002153"
     CDS             20921..21592
                     /gene="nfi"
                     /locus_tag="JQH51_002153"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY6076487.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21635..22225
                     /locus_tag="JQH51_002154"
     CDS             21635..22225
                     /locus_tag="JQH51_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY6076488.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22412..22684
                     /gene="hupA"
                     /locus_tag="JQH51_002155"
     CDS             22412..22684
                     /gene="hupA"
                     /locus_tag="JQH51_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY6076489.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22697..23392
                     /locus_tag="JQH51_002156"
     CDS             22697..23392
                     /locus_tag="JQH51_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY6076490.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23394..23819)
                     /gene="zraP"
                     /locus_tag="JQH51_002157"
     CDS             complement(23394..23819)
                     /gene="zraP"
                     /locus_tag="JQH51_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY6076491.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24057..25433
                     /gene="zraS"
                     /locus_tag="JQH51_002158"
     CDS             24057..25433
                     /gene="zraS"
                     /locus_tag="JQH51_002158"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY6076492.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25430..26755
                     /gene="zraR"
                     /locus_tag="JQH51_002159"
     CDS             25430..26755
                     /gene="zraR"
                     /locus_tag="JQH51_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY6076493.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26752..28041)
                     /gene="purD"
                     /locus_tag="JQH51_002160"
     CDS             complement(26752..28041)
                     /gene="purD"
                     /locus_tag="JQH51_002160"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY6076494.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28053..29642)
                     /gene="purH"
                     /locus_tag="JQH51_002161"
     CDS             complement(28053..29642)
                     /gene="purH"
                     /locus_tag="JQH51_002161"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY6076495.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7827 c         8261 g         6909 t
ORIGIN      
        1 cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg caatgcgcac
       61 taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct ggctcatcag
      121 tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta taatccgaaa
      181 agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat gtttgtagaa
      241 aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc gggcgcggcc
      301 tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggcggcgatt gtcggcaact
      361 acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt ctgattgctg
      421 cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccattgct tttgcccgtg
      481 aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca ttgcacacca
      541 cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga ctggatggta
      601 ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc tgaagctcct
      661 aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt agcaacgtcg
      721 ctgcgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt catggtacca
      781 accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg taaattcttc
      841 cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcactt ggtgcgcagc
      901 gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc aatcagcgat
      961 aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc gcgtccgaaa
     1021 acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc tgacttcaac
     1081 ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt ttctatcttc
     1141 ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta acccagcgat
     1201 caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa tttcgcgcct
     1261 tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag cctctcagag
     1321 gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc tatgtcaagc
     1381 tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct ctgggtcagc
     1441 agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat tccatcgaaa
     1501 aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc actttcgtta
     1561 ccaagacccc gccggcagca gttctgctga aaaaagcggc aggtatcaag tctggttccg
     1621 gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag gaaatcgcgc
     1681 agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc tccatcgaag
     1741 gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa ctgaccaagc
     1801 gcatgcgtgt tatccgtgag aaagttgatg caaccaaaca gtacgacatc aacgaagcta
     1861 tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac gtagctgtta
     1921 acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact gtactgccgc
     1981 acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaac gctgaagctg
     2041 ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag atcaagaaag
     2101 gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt gttggccagc
     2161 tgggccaggt tctgggtccg cgtggcctga tgccaaaccc gaaagtgggt actgtaacac
     2221 cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac cgtaacgaca
     2281 aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac aaactgaaag
     2341 aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag gcgaaaggcg
     2401 tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca gttgaccagg
     2461 ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt gtctacaatc
     2521 ttacccccac gtataatgct taatgcagac gtatatccga gatattcggg ttgtggcaag
     2581 gcggcaactg agtgagtcgc caagagcata gctaactatg tgactggtgc gaatgaagga
     2641 agccaacgcc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg ttggagcctg
     2701 gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa tcccctgcgt
     2761 agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct gctcaccgta
     2821 attaagacgc tctctccgtt tggaggagtg aagtgagttc cagagatttt ctctggcaaa
     2881 catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat tgttgctgaa
     2941 gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg tggcgtaact
     3001 gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata catgcgtgtt
     3061 gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg cctgaaagac
     3121 gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc tgctgctcgt
     3181 ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc tgcctttgaa
     3241 ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac ctacgaagaa
     3301 gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggcaaact ggttcgtact
     3361 ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt ttaacgcatt
     3421 cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat cactaaagat
     3481 caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact gatctctgca
     3541 atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc tggcccggtt
     3601 gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg cgctaacaaa
     3661 gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga agctaaagac
     3721 ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga cgcagaagca
     3781 ctgaaaaaag ctctggaaga agctggcgct gaagttgaag ttaaataagc caacccttcc
     3841 ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttagtcacca gcctttttgc
     3901 gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt tcaatgcttg
     3961 tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa tcgcaatgaa
     4021 atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc ggtcaacaaa
     4081 atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca gcgagctgag
     4141 gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt ttggtaaacg
     4201 tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt ttcagaaatt
     4261 tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt ccgtattccc
     4321 gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc ttggcgaacc
     4381 ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac cgctgcgcgt
     4441 taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa aagacattaa
     4501 agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta cctttgttat
     4561 caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg tcttctttga
     4621 ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc gcatcatccc
     4681 ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt tcgtacgtat
     4741 cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact acaccacaga
     4801 gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata acaagctgca
     4861 gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca tcttttgaca tcgaagctaa
     4921 cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc gccagctgga
     4981 aaaagacgac gtcaaactga ttgaagtccc ggttgagtac atcgcaggta aagtggttgc
     5041 taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca tggagctgag
     5101 cctggatctg ctggctaagc tgagccagtc tggtcacaag cgtatcgaaa cgctgttcac
     5161 caacgatctg gatcacggcc catatatctc tgaaacctta cgtgtcgacc caactaacga
     5221 ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc cgccgactcg
     5281 tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt atgacttgtc
     5341 tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg aaggttccgg
     5401 tattctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata tccgtaacgg
     5461 taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt ccgttggcga
     5521 aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg tgaaagagcg
     5581 tctgtctctg ggcgatctgg ataccctgat gcctcaggat atgatcaacg ccaagccgat
     5641 ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta tggaccagaa
     5701 caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc caggcggtct
     5761 gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcact acggtcgcgt
     5821 atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctc tgtccgtgta
     5881 cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaagtga ccgacggtgt
     5941 tgtaactgac gaaattcact acctgtctgc tatcgaagaa ggcaactacg ttatcgccca
     6001 ggcgaactcc aacctggatg aagaaggcca cttcgtagaa gacctggtaa cttgccgtag
     6061 caaaggcgaa tccagcttgt tcagccgcga ccaggttgac tacatggacg tatccaccca
     6121 gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg acgccaaccg
     6181 tgcattgatg ggtgcgaata tgcaacgtca ggccgttccg actctgcgcg ctgataagcc
     6241 gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa ctgcggtagc
     6301 taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca aagttaacga
     6361 agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca aatacacccg
     6421 ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg aaccggttga
     6481 acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg cgcttggtca
     6541 gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact ccatcctcgt
     6601 atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg aactggcgtg
     6661 tgtgtcccgt gacaccaagc tgggtccgga agagatcacc gctgacatcc cgaacgtggg
     6721 tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg cggaagtgac
     6781 cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc tgaccccaga
     6841 agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag actcttctct
     6901 gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta ctcgcgatgg
     6961 cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac aggcgaagaa
     7021 agacctgtct gaagaactgc agatcctcga agcgggtctg ttcagccgta tccgtgctgt
     7081 gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg atcgctggct
     7141 ggagctgggc ctgacagacg aagagaaaca aaatcagctg gaacagctgg ctgagcagta
     7201 tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca aaatcaccca
     7261 gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtatatctgg cggttaaacg
     7321 ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg taatttctaa
     7381 gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag acatcgtact
     7441 gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa cccacctggg
     7501 tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc agcaagaagt
     7561 cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg ttcgtcagaa
     7621 agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa acctgcgcaa
     7681 aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa ttaaagagct
     7741 gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg gtcgcactgg
     7801 tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac tgaaccacct
     7861 ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta ctcagcagcc
     7921 gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag tgtgggcgct
     7981 ggaagcatac ggcgcagcat acaccctgca ggaaatgctc actgttaagt ctgatgacgt
     8041 gaacggtcgt accaagatgt ataaaaacat tgtggacggc aaccatcaga tggagccggg
     8101 catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta tcaacatcga
     8161 actggaagac gagtaattct cgctcaaaca ggtcactgct gtcggggtaa aacccggcag
     8221 cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag tttctgaaag
     8281 cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg ccagacatga
     8341 tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac cgtacgttca
     8401 aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa gattacgagt
     8461 gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag aagtgcggcg
     8521 ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa ctggcttccc
     8581 cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg ctgctcgata
     8641 tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt atcgaaggcg
     8701 gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg gacgcgctgg
     8761 aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag gctctgctga
     8821 agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac gaaaccaact
     8881 ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg ttcgttcagt
     8941 ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg ccagatctgc
     9001 gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac gatctgtatc
     9061 gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct gcgccggaca
     9121 tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg ctggataacg
     9181 gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct ttggccgaca
     9241 tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt gttgactact
     9301 ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc ggtctgccga
     9361 agaaaatggc gctggagctg ttcaaaccgt tcatctacgg caagctggaa ctgcgtggtc
     9421 ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct gtcgtttggg
     9481 atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca ccgactctgc
     9541 accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct atccagctgc
     9601 acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg gctgttcacg
     9661 taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct accaacaaca
     9721 tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt gtactgggtc
     9781 tgtactacat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg ctgactggcc
     9841 cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg cgcgttaaag
     9901 tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt agtagcgaaa accagcctga
     9961 aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt ctgccttact
    10021 ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac acctgctacc
    10081 gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac accggcttcg
    10141 cctatgccgc gcgttctggt gcatctgttg gcatcgatga catggtcatc ccggagaaga
    10201 aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag cagttccagt
    10261 ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg gctgcggcga
    10321 acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtt attaaccgtg
    10381 acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc gactccggtg
    10441 cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg atggcaaagc
    10501 cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt ctgaacgtac
    10561 tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc gcactgaaaa
    10621 ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac ctggtggtta
    10681 ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc gaggggggtg
    10741 acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa gacgttctga
    10801 agccgggtac tgctgatatt ctcgttccgc gcaacacgct gctgcacgaa cagtggtgtg
    10861 acctgctgga agagaactct gtcgacgcgg taaaagtacg ttctgttgta tcttgtgaca
    10921 ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc cacatcatca
    10981 acaaaggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg ggtacacagc
    11041 tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct gaatccagca
    11101 tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt gtgaactcca
    11161 gcggtaaact ggttatcact tcccgtaaca ccgaactgaa actgatcgac gaattcggtc
    11221 gtaccaaaga aagctacaaa gtgccttacg gtgcggtact ggcgaaaggc gatggcgaac
    11281 aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg gttatcaccg
    11341 aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt actcgtcaga
    11401 ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa cgtaccgcag
    11461 gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac gacgttctga
    11521 tcccaggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt gttcagctgg
    11581 aagatggcgt acagatcagc tctggtgaca ccttggcgcg tattccgcag gaatccggcg
    11641 gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa gctcgtcgtc
    11701 cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt aaagaaacca
    11761 aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac gaagagatga
    11821 ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt ggtgacgtaa
    11881 tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt catgctgtga
    11941 ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgta aagattaacg
    12001 ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc gttaacgcgg
    12061 gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag atcgcaaacc
    12121 gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg ctgggtatca
    12181 ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag gagaccactc
    12241 gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc ctgaaagaga
    12301 acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac caggatcgta
    12361 tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca gaagacgcat
    12421 ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac gagtaatcgt
    12481 taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatgggtg ttattcagag
    12541 ggcaaattct tcacggaaaa tattaagaaa ggcagggcga aacgaggaag aagcttttcg
    12601 ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct ttcaacaacc
    12661 ttgcgttttt catcttccgt agcggctcgg aataaattta cgttcttaca ttccgtctca
    12721 tagtctttgc gaggcgcgtc ccagatagct gtcccagtct ttccataccg gctgcaaacc
    12781 ctgagcggtt aacacggcag caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg
    12841 ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga aggcgctgac
    12901 gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc gcgtggagag
    12961 tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca ctaactggcg
    13021 ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc gcggaaagga
    13081 gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt cggcaaccat
    13141 atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc ctatcttatc
    13201 aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt cctgtttttt
    13261 gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat gtctcctgat aaaccatcac
    13321 cccatccaga ccaagttgct ttaactcggc gtattccgtc tccgccagcg gttgcacttc
    13381 catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa agtaatccat
    13441 ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc ccatctcccg
    13501 tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct tgatgcgatt
    13561 actcatggaa aatccgcagt acgtgcagtc gttagcgcaa agattggaaa gataaagcgg
    13621 gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg cctgttgggc
    13681 cagttgttcc agatagccac tggcggcagg cgataacagc gccatcatgt cgtcgcgggt
    13741 gagttgcgag gcatttagcg cccgctctac gtcagcagcc gttttgccgt tgatacgcag
    13801 gcggatgtcg tcccagtcca gttgtcgcca gcgattgctg aaggttttca tgccgatgcc
    13861 tccagaaatc cggtcagcgg gctggtggca tgagcaaaat aactgcggct gcccggtccg
    13921 gactgacgtg ccagcaggcc tacttctacc gccagacgaa atgccttcgc catgttgacg
    13981 ggatcgtccg cgacggcaat cgccgtattc actaacaccg cgtcggcccc catttccagc
    14041 gcctgcgtgg catggctggg aacgccgatg ccagcatcga caaccaccgg aactgtggcc
    14101 tgctggataa taatctccag catggcacgg gtttccagtc cctgattcga gccaatcggc
    14161 gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca caataccgga
    14221 tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcgtttc ggcggctttc
    14281 agggtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc caattttagc
    14341 cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga aaatggcttc ttccgctgtt
    14401 ttcgccccgg aagtattcgg cagcagggcc acacccgccg cgataagcgg ttcgaggata
    14461 gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg
    14521 gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt gccggtaaac
    14581 agatgtgaat caaacgtttt gtccgcaata cgtaacatct caaccccctg caataacctg
    14641 aaaaagcagg atctggtcgc catcctgcac gatatgttgc gtccactgct cacgcgggac
    14701 gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag
    14761 ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca ggatctgcat
    14821 tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg cgccactggc
    14881 tcgacttacc gtcgaacagt cggagttctc ccgcaggcgt ctctataccg cttaataact
    14941 taatggcttc cagtgcctgc aaagtgccca tcaccccgac caccgggcca accacgcccg
    15001 ccgtgcggca gttgcgttct ggctgctggt tatctggcca caggcagcgg taacacccct
    15061 gctcccaggg cggtgtcagt accatcaact gaccgccaaa tccgaccgcg ctggcggtga
    15121 taagcggcgt gttgagtgcc acgcaggcgg cattaatctc ctggcgagtc gccatattgt
    15181 cggtacagtc gagcaccaca tcggcttgtg caaccgcatc tttcaacgcc tcacccgtta
    15241 accgttgttg taatgctgtc agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc
    15301 tgacctgcga tttcgggctg ttgatatctt cagtggtaaa gaggatttgt cgttgcagat
    15361 tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca gcccctgcca
    15421 ggtatagcgc agcaggtgta cccagcccgc caagaccaat aatcagcacc tggctgtcga
    15481 gcagtttttg ctgcccgtca agagcgatat cgtcgagcag aatttggcgg ctataacgca
    15541 taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc cgttgccaaa
    15601 cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac acccgttgct
    15661 atcaccgcag gcgcgcgtgc cagactgata ccgccaatcg ccacggtggg ataatccgcc
    15721 aggcgctcaa catgccgtgc caactgttcc agcccctgcg gtgcagaagg catctgtttg
    15781 gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg
    15841 tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc gcggatggca
    15901 ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc ctgatgcttg
    15961 atcgcgagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg gcccagcgca
    16021 attgccgcca cgacatcggc ttccacctct tcatcgcgcc gatctttgat gcgtagctgg
    16081 agagtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc caccaccggg
    16141 tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac gcttcctcct
    16201 tatgcaggta gatttctccg cctctggcac ggaagttctc cgacatatcc gccattccca
    16261 cttcaatggt ttgcgcggcg gcgtaatcgc gtacttcctg gctgattttc atcgagcaga
    16321 attttggccc acacatggag caaaaatggg cgactttgcc ggactcttgg ggcagggttt
    16381 catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg tcttcccagc
    16441 gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt
    16501 ttgccaggtc agcagcgtgg gcggcaatct tataagtgat aagcccctgc ttaacatctt
    16561 ctttattggg cagacccaga tgctcttttg gcgttacgta acagagcatc gcgcagccaa
    16621 accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc ggtgcaatat
    16681 cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg
    16741 tcatattgcg gcggatcatc tgcatcggca catggcctgg gccttcaatc atcacctgta
    16801 cgtcatattc ccaggcgatt ttggtcagtt cgcccagcgt atgcaattcg gcaaactgcg
    16861 cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa
    16921 cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg aaattttcct
    16981 gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg ataccggtca
    17041 ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata gtgaagtaat
    17101 ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt
    17161 cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg atcggcaccg
    17221 gactgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg gagagatcca
    17281 tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct tcttcgatgg
    17341 aagaggtgac cgccgagttg ccgatattgg cgttaacttt taccaggaag ttacggccaa
    17401 taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca cgtccggcag
    17461 caacttcatc acggacaaat tccgcagtga tattttccgg cagatgtgcg ccaaagctca
    17521 ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg ctcgcggccc atattctcgc
    17581 ggatggcgat gaattccatt tccggcgtga tgatgccctg gcgggcgtag tgcagttggg
    17641 tgacacggcg tcctgttttg gcgcgttttg gcgttaataa gccgctaaaa cgtagctcgt
    17701 cgagaccatc atctgccagc cgcgttctgg tgtaatcgga actgcgcacg gtaagttctt
    17761 cggtatcggc gcgcgcatcg atccacggct ggcgtagttt tgccagccct tgctgcacgt
    17821 taatggcgat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt
    17881 tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta agctggatct
    17941 cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg
    18001 gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc
    18061 gggtcagttt tgttgcagac atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac
    18121 gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg atagcagatt aactcttgtt
    18181 cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg tctcagccag
    18241 ttaaggcact ccgacaagaa attagccccc acaaacgggg cgttgaatgt aaattacgcg
    18301 ttaacagcgc agaactcaag caggatgttt gacgcgggcg gcgtcaagca ccagcagaat
    18361 cagcttatct tccagcacaa agcgcgcttc cagcgcctcg ccgatgtcag ataaaacctg
    18421 ttgaaactca aggtaattat catgatcgat ggcggtttcc aggctggaat cgtagtaatc
    18481 cataatctgt tgggtattgg cttcgagttg cggccaaatc ttcgcggcgc gcgcgagttg
    18541 cccgttgcct tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa
    18601 gtaatcgacc aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga
    18661 ttctttgcca ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac
    18721 atgtagccag cgatcaacca gtttgttact tcctctgacg cgctccgtca ggttatcgag
    18781 ctggttaagc atgattgact ccgcaagttt gtattcaaaa actgctcagt gagaaatgta
    18841 aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat
    18901 tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa
    18961 gggagaattg ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca
    19021 aatcggcgaa tggcaggggg aacctgtttg gttaatacaa cagcagcggc gtcacgatat
    19081 ggggtcggta cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg
    19141 cgtgcaactg gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat
    19201 gtatccgagc aaaaccgaat gggcgatgct gtgtagccat tgccgtgagc gttactaccc
    19261 gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat gattcgctcc tcctcgccca
    19321 gcatacccgc catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggcga
    19381 aaccctcgag caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa
    19441 cttgcgttac gtgacttctc agccgtggcc gtttcctcag tctttaatga ccgcgtttat
    19501 ggcggaatat gacagcggcg acatcgtgat cgacccgaaa gaattgctcg aggcgaactg
    19561 gtatcgctat gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat
    19621 agaagatact gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac
    19681 gcactaagga acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct
    19741 gcgccagccc gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc
    19801 ggaatataaa gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaacgccga
    19861 gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac ccgctggatg cggcgatcct
    19921 cttttccgat atcctcaccg tgccggacgc gatggggtta gggctctatt ttgaagccgg
    19981 agaaggtccg cgttttacct cgccagtcac ctgcaaagcc gacgtcgata aactgccaat
    20041 tccggacccg gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcacga
    20101 actgaaaggc gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcaaccta
    20161 catggtggaa ggcggcagca gcaaagcctt caccgtgatc aaaaaaatga tgtatgccga
    20221 tccgcaggcg ctgcacgctc tgctcgataa actggcgaaa agcgtcactt tgtatctgaa
    20281 tgcgcagatt aaagccggtg ctcaggcagt gatgattttc gacacctggg gcggcgtgct
    20341 taccgggcgc gattatcaac agttctcgct ctattacatg cataaaattg ttgatggttt
    20401 actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag gcggcggaca
    20461 gtggctggaa gccatggctg aaaccggttg cgatgcgctg ggcctcgact ggacaacgga
    20521 catcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg ttgcagggta atatggatcc
    20581 gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa gtagcgacta tacttgcagg
    20641 tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac ggcattcatc aggatgtgcc
    20701 gccagaacat gctggcgtgt tcgtggaggc agtgcatcga ctgtctgaac agtatcaccg
    20761 ctaagagcct gatccatcag gctattttat ttgccatttt gaggccgggc agtgctcaaa
    20821 atcctcacgt actacgtgta cgctccggtt tttccgcgct gtccggcctc aaactgcctt
    20881 caacaattac gcctgttgaa ccaggctcta aggagcgatt atggatctcg cgtcattacg
    20941 cgctcaacaa atcgaactgg cttcttctgt gatctgcgag gatcgactcg ataaagatcc
    21001 accggatctg atcgccggag ccgatgtcgg gtttgagcag ggcggagaag tgacgcgagc
    21061 ggcgatggtg ctgctgaaat atccctcgct tgagctggtg gagtataaag ttgcccgcat
    21121 cgccaccacc atgccttaca ttccaggttt tctttccttc cgcgaatatc ctgcgctgct
    21181 ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg gtcatgggat
    21241 ctcgcatcct cgccgtcttg gcgtcgccag ccattttggc ttattggtgg atgtgccgac
    21301 cattggcgtg gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcaaaccggg
    21361 cgcgctggcc ccactgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc
    21421 gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc
    21481 gtgggtacaa cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc
    21541 ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta
    21601 aactgcggat aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg
    21661 tctggagcgc ctggaaagct ggcagcatgt cactttcatg gcttgcttat gcgaacgcat
    21721 gtaccccaat tacgccatgt tctgccagca aaccggtttt ggtgatgggc aaatttaccg
    21781 tcgtattctc gatctcatct gggaaacgct gaccgttaaa gacgcaaaag taaatttcga
    21841 cagccaactg gagaaatttg aagaagcgat tccttcagcc gacgatttcg atctgtacgg
    21901 cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt cgcgtttgag
    21961 tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc tccatcacga ccgttgcgat
    22021 gctggaaatg actcaggctg gtcgcgaaat gggcgatgaa gagcttaaag aaaacccagc
    22081 tgtagagcaa gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga
    22141 acgcgatatc gagctgataa aaggccttag ggcagacctg cgtgagtcgg gtgagagcaa
    22201 tattggtata atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt
    22261 acctggagtc ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg
    22321 ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta
    22381 gcaagcgata aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat
    22441 tgcagagaaa gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc
    22501 tgcaattact gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt
    22561 caaagtgaac catcgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa
    22621 aattgccgca gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa
    22681 gtaagattgc gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt
    22741 ctggcgtcga tcattgatgc tggcaggtgc gctgtttctc actgcctgta gccacaactc
    22801 ttcacttcct ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg
    22861 gcgaaaagac agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg
    22921 cgataccacg acgcgagagt atcgctggca gggcgataac ctcacgctca tcaatatcaa
    22981 tgtttacagc aaaccgccgg tgaacattcg cgcgcgtttt gacgatcgcg gcgatctgag
    23041 ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt
    23101 ataccgttat cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt
    23161 cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca
    23221 aaccattaaa cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg
    23281 ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt
    23341 agtggcaaac tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag
    23401 tggcccatac ccatatgacc gccaccaccg cagccgccgt agcccatgcc cattccggct
    23461 ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt tcacccgtaa ctcatctaac
    23521 gactgacgca aattctccat ctctttggcg accgcgttaa ttttgctgct atccggtggg
    23581 ttcgcggcta acagggcatt gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg
    23641 ctgctttgag cgtaaaagtc attatggatt ttctgccacg ctgtctgttg ttcgctggtc
    23701 aaaggcgcgg cattttgctg ccacataccg tgtccgccgt gagcaaatgc agatgtcgat
    23761 cccatcgcca ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg
    23821 gttaatcctc cagtggttgt cttacttcgg gtattgcatc ttccgtgcca acgatgaaac
    23881 gctgatatga cgggtaatct ggcatgataa acgagtaaaa atgactcgcc tgctgcgggt
    23941 agcgagtcat ttttactcat tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct
    24001 gataaaagat ggcatgattt ctgctgtcag aaagggatga gcaggcaaag aagaagatgc
    24061 gttttatgca acgttctaaa gactccttag ctaaatggtt aagcgcgatc ctccccgtgg
    24121 tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga
    24181 ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt acttattcgc gctcttgaat
    24241 ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag gccttactgg
    24301 aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa
    24361 tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct ttattcccct caggaaatgc
    24421 agcagttaca tccgggagat gaagaagcgt ggcggcggat cgatagcgca gacggcgagc
    24481 ctgttctgga aatttatcgc cagtttcaac cgatgtttgc tgctggaatg taccggatgc
    24541 gccatatgca gcagtatgcc gcgacaccac aagcaatttt catcgctttc gacgccagta
    24601 atattgtgag tgccgaagat cgtgagcaga gaaacaccct gattattctc ttcgccctgg
    24661 cgacggtctt gctggcaagc gtattgtcat tcttctggta tcgccgctat ctgcgctcgc
    24721 gccagcttct acaagatgaa atgaagcgca aagagaagct ggtggcgctg gggcatcttg
    24781 cggcaggcgt tgcccacgaa atccgtaacc cactttcctc gattaaagga ctggcgaaat
    24841 actttgccga gcgcgcgcct gcagggggag aagcgcatca actggcgcag gtgatggcga
    24901 aagaggccga ccgtttaaac cgcgtggtaa gcgagttgct ggaactggtt aagccaacgc
    24961 atctggcttt gcaggcggtg gatctcaaca cgctgattaa ccactcatta cagctggtaa
    25021 gccaggatgc aaacagccgg gagatccagt tacgctttac cgccaacgac acattaccgg
    25081 aaattcaggc cgatccggac aggctgactc aggtcctgtt gaatctctat ctcaatgcta
    25141 ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg
    25201 tgaaaatcag cgttaccgac agcggtaagg gaattgcggc agatcagctt gaagccatct
    25261 tcactccgta cttcaccacc aaagccgaag gcaccggatt ggggctggcg gtcgtgcata
    25321 atattgttga acaacacggt ggtacaattc aggtcgcaag ccaggaggga aaaggctcaa
    25381 cgttcaccct ctggcttccg gtcaatatta cgcgtaagga cccacaagga tgacgcacga
    25441 taatatcgat attctggtgg tggatgatga cattagccac tgcactattt tgcaggcttt
    25501 actgcgcggc tggggctata acgtcgcgct ggcgaacagc gggcgacagg cgctggagca
    25561 ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg
    25621 catcgccacg ctgaaagaga tcaaagcgtt aaacccggca attccggtgc tgattatgac
    25681 tgcgtactcc agcgtcgaga cggcggtaga ggcactgaaa actggggcgc aggattatct
    25741 catcaagccg ctggatttcg ataacctaca ggcgacgctg gaaaaggcgc tcgcgcatac
    25801 gcacagtatt gatgctgaaa cacctgcggt gactgccagc cagttcggta tggtcggtaa
    25861 aagcccggcg atgcaacacc tgctcagtga aatcgccctc gtcgcgccat cggaagccac
    25921 ggtgctgatc cacggcgatt ccggcaccgg taaagagctg gtcgccaggg cgattcacgc
    25981 cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc
    26041 cttgctggaa tctgaattgt tcggtcacga aaaaggggcg tttactggag ccgataaacg
    26101 ccgggaaggg cgctttgttg aggcggacgg cggcacgctg tttctcgatg aaattggcga
    26161 tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg
    26221 tgtcggtagc aaccagacta tctcggttga tgtccggctg attgcggcga cccatcgcga
    26281 tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat ctctactatc gcctgaatgt
    26341 ggtggcgatt gaagtaccat cgctgcggca acggcgggaa gatatccctc tgctggctgg
    26401 ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca
    26461 ggcgatggat ttgctgatcc actacgactg gccgggaaat attcgtgaac tggaaaacgc
    26521 ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg agctgccgct
    26581 ggcgattgcc agtacgccga tcccgctggg gcaaagtcag gatattcagc cgctggtgga
    26641 agtggaaaaa gaggtgattc tggcggcgct ggagaaaacg ggcggcaaca aaaccgaagc
    26701 cgcccgtcag ttagggatca cgcgcaaaac gctattggca aaactgtcgc gttagttctg
    26761 ctcgcgttcg atggcgcgcc aaccgatatc tttccggcag aagcaatcat cccagtggat
    26821 atcggtcatt aaggcgtagg cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt
    26881 gacgcacagt acgcgcccgc cgctggttac cacctgctcg tcatccgcca gttttgtgcc
    26941 cgcgtggaac actttgccgt cagccacttc ttccagcggc aggccgtgga tcacatcgcc
    27001 ggtgcggtaa tcgcccggat atccacccgc agccatcacc acgctgagag aagcacgttc
    27061 atcccactct gacgttttct cgtccagttt gccttcacag gccgccaggc agagctcaac
    27121 cagatcggac ttcatgcgca gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt
    27181 aaattcaata accttcggat tgccctgttt gtcgatcatc aggcccgcat agagaaaacc
    27241 ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt ggccagatga tacgttccat
    27301 ggtgcgctga tgaacttcat cggttactac cggcgcgggg gagtaagcgc ccatcccgcc
    27361 ggtgtttggt ccgttatctt tatcgcctac gcgtttgtga tcctggctgg tagccatcgg
    27421 cagcacatgc tcgccgtcca ccatcacgat aaagctcgct tcttcgccat cgaggaactc
    27481 ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac
    27541 agccgcttcc gcttcttcca gcgtcatcgc cacgataacg cctttcccgg cagccagacc
    27601 gtccgcttta atgacgattg gcgcgccttt ctcacgcaga tacaccagcg caggttctac
    27661 ctcggtgaag ttctggtatt ccgccgtagg gatcttatgg cgggccagga aatctttggt
    27721 aaacgctttt gagccttcca gttgggccgc acctgcggtt gggccgaaga ttttcagccc
    27781 ggcggcgcgg aaggtatcga ccacgccttt caccagcggc gcttccgggc cgacgatggt
    27841 cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat
    27901 agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca ttacccggag cagcaaaaac
    27961 agtctcaacc agcggcgact gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt
    28021 accaatcact aatactttca tctattgctc cattaatggc ggaagtggcg catgtcggtg
    28081 aagagcatcg caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa
    28141 ccgccaggct ggattacgca ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg
    28201 aacgggaaga acgcgtcaga agccatcgag gaacctttca cttccaggcc ttcatcggca
    28261 gctttaatac cggcgatttt cgcggagtac acgcggctca tctggcccgc gccaataccg
    28321 atggtcatat tgtttttggc atagacgata gcattggatt tcacgaattt cgccactttc
    28381 cagcagaaca gcgcatcacg cagttcctgt tcggtcggct gacgttgggt gacgacgcgc
    28441 agttcttccg caccgaccat ccccaggtca cgatcctgaa ccagcagacc gccgttcacg
    28501 cgtttgaaat caagacccgg aacacgctcg ccccactgac cgcaggtcag aacgcgtacg
    28561 ttctgcttgg cggcggtgat tttcagggct tcttcgctgg cggaaggcgc aataatcact
    28621 tcgacaaact gacgagaaat gatggcctgt gcggtttccg catccagctc gcggttaaag
    28681 gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc gtaagcatca
    28741 agaatggaat tgccgatagc tacaccgcaa gggttggcgt gcttcacaat cacacatgcc
    28801 ggctcggcga actctttcac gcactccagc gccgcatcgg tatcggcgat gttgttataa
    28861 gagagggctt taccctgaac ctgggttgcg gtagcaacgg aggcttcttt cacattctct
    28921 tctatataga aggcagcctg ctggtggctg ttctcgccgt aacgcatatc ctgcttctta
    28981 atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg
    29041 tacgccggaa ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt
    29101 tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca
    29161 tccatctctt taataatggc gtcgtagtcg ctgctcttca ccacgattgc gacatcttta
    29221 tggttcttag cggcggagcg caccatggtc gggccgccga tatcgatgtt ctcaaccgca
    29281 tcttccagcg agcagccttc acgagcaaca gtctgggcga acggatacag gttaacaacc
    29341 accatatcga taggctggat ctgatgttct tccataatgg catcgtcctg gccgcgacgg
    29401 cccagaatgc caccatgtac tttcggatgc agggtcttca cgcgtccatc catcatctcc
    29461 gggaaaccgg tgtaatcgga aacttcggtt accggcagac ctttttctgc taacagacgg
    29521 gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg
    29581 acgataccgg ctttgtcaga aacactgagc agagcgcggc ggactggacg acgttgttgc
    29641 atggtaaatc ccctgggttt gactattaca gagagcgtta gctgaatttt tcgcgaaaaa
    29701 ctcagctaac gcccctaacg gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt
    29761 tgcgcaacgc tcgcgaattt ttctctttca atggtgatca caattttgac agtggttacc
    29821 gtgggaaaaa tacagaaatt acattgatga ttgtggataa ctctgtgcgt aaaaaggtat
    29881 aaagcggggt tttgctgggg aatgcagcag tcagtcattt ttctgcaatt tttctattgc
    29941 ggcctgcgga gaactcccta taatgcgcct tcatcgacac ggcggatgtg aatcacttca
    30001 cacaaacagc cggttcggtt gaaga
//
LOCUS       DADLEE010000046        29797 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000046 DADLEE010000000
VERSION     DADLEE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.046"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            56..850
                     /locus_tag="JQH51_002162"
     CDS             56..850
                     /locus_tag="JQH51_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY6076496.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="JQH51_002163"
     CDS             872..2281
                     /locus_tag="JQH51_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076497.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="JQH51_002164"
     CDS             2259..3038
                     /locus_tag="JQH51_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY6076498.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3002..3895
                     /locus_tag="JQH51_002165"
     CDS             3002..3895
                     /locus_tag="JQH51_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY6076499.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIG
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="JQH51_002166"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="JQH51_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="JQH51_002167"
     CDS             complement(4635..4895)
                     /locus_tag="JQH51_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY6076500.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="JQH51_002168"
     CDS             complement(4886..6157)
                     /locus_tag="JQH51_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6076501.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JQH51_002169"
     CDS             complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JQH51_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY6076502.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="JQH51_002170"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="JQH51_002170"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY6076503.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="JQH51_002171"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="JQH51_002171"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY6076504.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="JQH51_002172"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="JQH51_002172"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY6076505.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="JQH51_002173"
     CDS             11499..11651
                     /locus_tag="JQH51_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6076506.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12996
                     /locus_tag="JQH51_002174"
     CDS             11845..12996
                     /locus_tag="JQH51_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY6076507.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGIDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAARFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JQH51_002175"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="JQH51_002175"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY6076508.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="JQH51_002176"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="JQH51_002176"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY6076509.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="JQH51_002177"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="JQH51_002177"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY6076510.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="JQH51_002178"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="JQH51_002178"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY6076511.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="JQH51_002179"
     CDS             17363..17686
                     /locus_tag="JQH51_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY6076512.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="JQH51_002180"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="JQH51_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY6076513.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="JQH51_002181"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="JQH51_002181"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY6076514.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="JQH51_002182"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="JQH51_002182"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY6076515.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="JQH51_002183"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="JQH51_002183"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY6076516.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="JQH51_002184"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="JQH51_002184"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY6076517.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="JQH51_002185"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="JQH51_002185"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY6076518.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="JQH51_002186"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="JQH51_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY6076519.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="JQH51_002187"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="JQH51_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY6076520.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="JQH51_002188"
     CDS             complement(24261..24668)
                     /locus_tag="JQH51_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY6076521.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="JQH51_002189"
     CDS             24815..25408
                     /locus_tag="JQH51_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY6076522.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="JQH51_002190"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="JQH51_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="JQH51_002191"
     CDS             complement(26628..27191)
                     /locus_tag="JQH51_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY6076523.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="JQH51_002192"
     CDS             27356..27607
                     /locus_tag="JQH51_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6076524.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="JQH51_002193"
     CDS             27609..29705
                     /locus_tag="JQH51_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY6076525.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7295 a         7383 c         8062 g         7057 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacgga acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgta tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaaccctttg cagggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatga
//
LOCUS       DADLEE010000047        29468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000047 DADLEE010000000
VERSION     DADLEE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.047"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..213)
                     /locus_tag="JQH51_002194"
     CDS             complement(<1..213)
                     /locus_tag="JQH51_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY6076526.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JQH51_002195"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JQH51_002195"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY6076527.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JQH51_002196"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JQH51_002196"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY6076528.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JQH51_002197"
     CDS             2894..3634
                     /locus_tag="JQH51_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6076529.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JQH51_002198"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JQH51_002198"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY6076530.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="JQH51_002199"
     CDS             complement(4192..4794)
                     /locus_tag="JQH51_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY6076531.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /locus_tag="JQH51_002200"
     CDS             4864..4929
                     /locus_tag="JQH51_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076532.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="JQH51_002201"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="JQH51_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY6076533.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="JQH51_002202"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="JQH51_002202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY6076534.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="JQH51_002203"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="JQH51_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY6076535.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="JQH51_002204"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="JQH51_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY6076536.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="JQH51_002205"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="JQH51_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY6076537.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="JQH51_002206"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="JQH51_002206"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY6076538.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="JQH51_002207"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="JQH51_002207"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY6076539.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="JQH51_002208"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="JQH51_002208"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY6076540.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /locus_tag="JQH51_002209"
     CDS             complement(13523..14392)
                     /locus_tag="JQH51_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY6076541.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="JQH51_002210"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="JQH51_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY6076542.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="JQH51_002211"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="JQH51_002211"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY6076543.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /locus_tag="JQH51_002212"
     CDS             18287..18928
                     /locus_tag="JQH51_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY6076544.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="JQH51_002213"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="JQH51_002213"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY6076545.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="JQH51_002214"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="JQH51_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="JQH51_002215"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="JQH51_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY6076546.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="JQH51_002216"
     CDS             21336..21995
                     /locus_tag="JQH51_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY6076547.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="JQH51_002217"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="JQH51_002217"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY6076548.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="JQH51_002218"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="JQH51_002218"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY6076549.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /locus_tag="JQH51_002219"
                     /pseudo
     CDS             complement(24948..25604)
                     /locus_tag="JQH51_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25706..25936)
                     /locus_tag="JQH51_002220"
     CDS             complement(25706..25936)
                     /locus_tag="JQH51_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY6076550.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..26449
                     /gene="yobA"
                     /locus_tag="JQH51_002221"
     CDS             26075..26449
                     /gene="yobA"
                     /locus_tag="JQH51_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY6076551.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26453..27325
                     /gene="copD"
                     /locus_tag="JQH51_002222"
                     /pseudo
     CDS             26453..27325
                     /gene="copD"
                     /locus_tag="JQH51_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27338..27679
                     /locus_tag="JQH51_002223"
     CDS             27338..27679
                     /locus_tag="JQH51_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY6076552.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28072..28438)
                     /locus_tag="JQH51_002224"
                     /pseudo
     CDS             complement(28072..28438)
                     /locus_tag="JQH51_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(28757..29149)
                     /locus_tag="JQH51_002225"
     CDS             complement(28757..29149)
                     /locus_tag="JQH51_002225"
                     /inference="COORDINATES: protein motif:HMM:NF020572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076553.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMAGWIERYKTILIRRKASRNTYKTRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDL"
     gene            complement(29115..29396)
                     /locus_tag="JQH51_002226"
     CDS             complement(29115..29396)
                     /locus_tag="JQH51_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY6076554.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7438 a         7235 c         7568 g         7227 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac
    26221 tttaaacttc tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa
    26281 aaacgaaaat attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat
    26341 tgttccactg gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc
    26401 ggtggacggg cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc
    26461 gtttacctgg atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt
    26521 tgcaatgtat ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc
    26581 tctgcgttta caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc
    26641 cgttcagggc gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca
    26701 ggctgttttg caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt
    26761 aacgttgatc gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac
    26821 taccgcgcaa tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac
    26881 agcgaaaatc catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg
    26941 gggattactg ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc
    27001 tattcaggcg ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc
    27061 aagcggcgtg cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg
    27121 gggccaactc ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc
    27181 taatcgttat gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt
    27241 tgtgtggatg actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct
    27301 gcttgcgact ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc
    27361 gtttctgtta ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag
    27421 tcgttttgaa gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg
    27481 ccgaccgggt aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa
    27541 cgatatcgca caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat
    27601 tcagattgat gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg
    27661 agctgaaaaa ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc
    27721 tcagttcttt caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt
    27781 tattgaccat tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca
    27841 aggcaactaa gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt
    27901 tccctggacc gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt
    27961 agttgcaaac gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt
    28021 ttttggttat atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag
    28081 taagtacgtt ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc
    28141 aagtagacgc tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct
    28201 tctgatttcg tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg
    28261 ctgcgacaaa tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc
    28321 taatcccggc actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg
    28381 caccaagtcg taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc
    28441 ctgtcttaat ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg
    28501 ttatgtcttc gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg
    28561 ccggaagttg atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc
    28621 ttgtaactac tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt
    28681 caacaatggc ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt
    28741 ttttccctcc ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg
    28801 tcagtaaaac ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat
    28861 aagtatttct ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg
    28921 ccatagtaaa tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt
    28981 tgtatatata atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac
    29041 ctagtgtaaa ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc
    29101 gatatgttaa gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc
    29161 tccattaacg gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta
    29221 tcaaccttat ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt
    29281 agttcaggtg caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat
    29341 ggtgggttat ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc
    29401 atacctctca cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt
    29461 ttaataat
//
LOCUS       DADLEE010000048        29195 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000048 DADLEE010000000
VERSION     DADLEE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29195
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.048"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="JQH51_002227"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="JQH51_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY6076555.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="JQH51_002228"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="JQH51_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY6076556.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQH51_002229"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQH51_002229"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY6076557.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="JQH51_002230"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="JQH51_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY6076558.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="JQH51_002231"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="JQH51_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY6076559.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="JQH51_002232"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="JQH51_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY6076560.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="JQH51_002233"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="JQH51_002233"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY6076561.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="JQH51_002234"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="JQH51_002234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY6076562.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="JQH51_002235"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="JQH51_002235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY6076563.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="JQH51_002236"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="JQH51_002236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY6076564.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="JQH51_002237"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="JQH51_002237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY6076565.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="JQH51_002238"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="JQH51_002238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY6076566.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="JQH51_002239"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="JQH51_002239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY6076567.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="JQH51_002240"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="JQH51_002240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY6076568.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="JQH51_002241"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="JQH51_002241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY6076569.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="JQH51_002242"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="JQH51_002242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY6076570.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="JQH51_002243"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="JQH51_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY6076571.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="JQH51_002244"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="JQH51_002244"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY6076572.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="JQH51_002245"
     CDS             19508..20329
                     /locus_tag="JQH51_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HAY6076573.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="JQH51_002246"
     CDS             20316..22424
                     /locus_tag="JQH51_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY6076574.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="JQH51_002247"
     CDS             22421..24088
                     /locus_tag="JQH51_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY6076575.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="JQH51_002248"
     CDS             24091..25617
                     /locus_tag="JQH51_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY6076576.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="JQH51_002249"
     CDS             25618..27234
                     /locus_tag="JQH51_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY6076577.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKSDRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="JQH51_002250"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="JQH51_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="JQH51_002251"
     CDS             complement(27465..27842)
                     /locus_tag="JQH51_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076578.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="JQH51_002252"
     CDS             complement(28252..28623)
                     /locus_tag="JQH51_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076579.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="JQH51_002253"
     CDS             complement(28684..29181)
                     /locus_tag="JQH51_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076580.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
BASE COUNT         7540 a         6813 c         7514 g         7328 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataagtcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagct
//
LOCUS       DADLEE010000049        29043 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000049 DADLEE010000000
VERSION     DADLEE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.049"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="JQH51_002254"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="JQH51_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY6076581.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="JQH51_002255"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="JQH51_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY6076582.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="JQH51_002256"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="JQH51_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY6076583.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="JQH51_002257"
     CDS             4843..5082
                     /locus_tag="JQH51_002257"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076584.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="JQH51_002258"
     CDS             5092..5262
                     /locus_tag="JQH51_002258"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY6076585.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6303
                     /locus_tag="JQH51_002259"
                     /pseudo
     CDS             5311..6303
                     /locus_tag="JQH51_002259"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            6355..6990
                     /locus_tag="JQH51_002260"
                     /pseudo
     CDS             6355..6990
                     /locus_tag="JQH51_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
     gene            7084..7773
                     /locus_tag="JQH51_002261"
     CDS             7084..7773
                     /locus_tag="JQH51_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY6076586.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="JQH51_002262"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="JQH51_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY6076587.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="JQH51_002263"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="JQH51_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY6076588.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="JQH51_002264"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="JQH51_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY6076589.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="JQH51_002265"
     CDS             complement(10475..12193)
                     /locus_tag="JQH51_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY6076590.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="JQH51_002266"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="JQH51_002266"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY6076591.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="JQH51_002267"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="JQH51_002267"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY6076592.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="JQH51_002268"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="JQH51_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY6076593.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="JQH51_002269"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="JQH51_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY6076594.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="JQH51_002270"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="JQH51_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="JQH51_002271"
     CDS             18432..18746
                     /locus_tag="JQH51_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY6076595.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="JQH51_002272"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="JQH51_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY6076596.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="JQH51_002273"
     CDS             20570..21862
                     /locus_tag="JQH51_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY6076597.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="JQH51_002274"
     CDS             complement(22016..23665)
                     /locus_tag="JQH51_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY6076598.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /locus_tag="JQH51_002275"
     CDS             complement(23816..25165)
                     /locus_tag="JQH51_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6076599.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="JQH51_002276"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="JQH51_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY6076600.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="JQH51_002277"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="JQH51_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY6076601.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="JQH51_002278"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="JQH51_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="JQH51_002279"
     CDS             28753..29034
                     /locus_tag="JQH51_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076602.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7352 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DADLEE010000050        28914 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000050 DADLEE010000000
VERSION     DADLEE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.050"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQH51_002280"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQH51_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY6076603.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="JQH51_002281"
     CDS             complement(2138..2584)
                     /locus_tag="JQH51_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY6076604.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="JQH51_002282"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="JQH51_002282"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY6076605.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQH51_002283"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQH51_002283"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY6076606.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="JQH51_002284"
     CDS             complement(5347..6009)
                     /locus_tag="JQH51_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY6076607.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="JQH51_002285"
     CDS             6182..6595
                     /locus_tag="JQH51_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY6076608.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQH51_002286"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQH51_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY6076609.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQH51_002287"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQH51_002287"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY6076610.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="JQH51_002288"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="JQH51_002288"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY6076611.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQH51_002289"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQH51_002289"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY6076612.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQH51_002290"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQH51_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY6076613.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="JQH51_002291"
     tRNA            complement(9861..9948)
                     /locus_tag="JQH51_002291"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="JQH51_002292"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="JQH51_002292"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY6076614.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="JQH51_002293"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="JQH51_002293"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY6076615.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="JQH51_002294"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="JQH51_002294"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY6076616.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="JQH51_002295"
     CDS             14005..14592
                     /locus_tag="JQH51_002295"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY6076617.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="JQH51_002296"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="JQH51_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY6076618.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="JQH51_002297"
     CDS             14984..15841
                     /locus_tag="JQH51_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY6076619.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="JQH51_002298"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="JQH51_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY6076620.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="JQH51_002299"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="JQH51_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY6076621.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="JQH51_002300"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="JQH51_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY6076622.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /locus_tag="JQH51_002301"
     CDS             18851..20149
                     /locus_tag="JQH51_002301"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY6076623.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="JQH51_002302"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="JQH51_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="JQH51_002303"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="JQH51_002303"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY6076624.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="JQH51_002304"
     CDS             complement(22884..24023)
                     /locus_tag="JQH51_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY6076625.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="JQH51_002305"
     CDS             complement(24069..26165)
                     /locus_tag="JQH51_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY6076626.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="JQH51_002306"
     CDS             complement(26165..26911)
                     /locus_tag="JQH51_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY6076627.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="JQH51_002307"
     CDS             complement(26908..27552)
                     /locus_tag="JQH51_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY6076628.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="JQH51_002308"
     CDS             complement(27659..27964)
                     /locus_tag="JQH51_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076629.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="JQH51_002309"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="JQH51_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY6076630.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7319 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gagaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DADLEE010000051        28669 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000051 DADLEE010000000
VERSION     DADLEE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28669
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.051"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            163..648
                     /locus_tag="JQH51_002310"
     CDS             163..648
                     /locus_tag="JQH51_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076631.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="JQH51_002311"
     CDS             645..1031
                     /locus_tag="JQH51_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076632.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="JQH51_002312"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="JQH51_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY6076633.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3292..3737
                     /locus_tag="JQH51_002313"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="JQH51_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="JQH51_002314"
     CDS             3759..4166
                     /locus_tag="JQH51_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY6076634.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="JQH51_002315"
     CDS             4163..6451
                     /locus_tag="JQH51_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY6076635.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="JQH51_002316"
     CDS             6448..7437
                     /locus_tag="JQH51_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY6076636.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="JQH51_002317"
     CDS             7537..7740
                     /locus_tag="JQH51_002317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076637.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="JQH51_002318"
     CDS             7758..8078
                     /locus_tag="JQH51_002318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076638.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="JQH51_002319"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="JQH51_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="JQH51_002320"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="JQH51_002320"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY6076639.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9410..9883
                     /gene="ivy"
                     /locus_tag="JQH51_002321"
     CDS             9410..9883
                     /gene="ivy"
                     /locus_tag="JQH51_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY6076640.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /locus_tag="JQH51_002322"
     CDS             complement(9926..12370)
                     /locus_tag="JQH51_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY6076641.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="JQH51_002323"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="JQH51_002323"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY6076642.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="JQH51_002324"
     CDS             13394..14161
                     /locus_tag="JQH51_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY6076643.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="JQH51_002325"
     CDS             complement(14132..14872)
                     /locus_tag="JQH51_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY6076644.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15164..15777
                     /locus_tag="JQH51_002326"
                     /pseudo
     CDS             15164..15777
                     /locus_tag="JQH51_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="JQH51_002327"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="JQH51_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY6076645.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18406)
                     /gene="flhA"
                     /locus_tag="JQH51_002328"
     CDS             complement(16667..18406)
                     /gene="flhA"
                     /locus_tag="JQH51_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY6076646.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSTLLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIEADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLPRYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="JQH51_002329"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="JQH51_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY6076647.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="JQH51_002330"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="JQH51_002330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY6076648.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="JQH51_002331"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="JQH51_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY6076649.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="JQH51_002332"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="JQH51_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY6076650.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="JQH51_002333"
     CDS             21018..21470
                     /locus_tag="JQH51_002333"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6076651.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="JQH51_002334"
     CDS             21716..22759
                     /locus_tag="JQH51_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY6076652.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22875..23435
                     /locus_tag="JQH51_002335"
                     /pseudo
     CDS             22875..23435
                     /locus_tag="JQH51_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="JQH51_002336"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="JQH51_002336"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY6076653.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="JQH51_002337"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="JQH51_002337"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY6076654.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="JQH51_002338"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="JQH51_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY6076655.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /locus_tag="JQH51_002339"
     CDS             27063..27464
                     /locus_tag="JQH51_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY6076656.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="JQH51_002340"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="JQH51_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY6076657.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7083 a         7121 c         7606 g         6859 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccacgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttcttgctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggtgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatatt
//
LOCUS       DADLEE010000052        28442 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000052 DADLEE010000000
VERSION     DADLEE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28442
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.052"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(20..178)
                     /locus_tag="JQH51_002341"
     CDS             complement(20..178)
                     /locus_tag="JQH51_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076658.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKSCRLAP"
     gene            359..1990
                     /gene="dcuS"
                     /locus_tag="JQH51_002342"
     CDS             359..1990
                     /gene="dcuS"
                     /locus_tag="JQH51_002342"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY6076659.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1987..2706
                     /gene="dcuR"
                     /locus_tag="JQH51_002343"
     CDS             1987..2706
                     /gene="dcuR"
                     /locus_tag="JQH51_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY6076660.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3277..4617
                     /locus_tag="JQH51_002344"
     CDS             3277..4617
                     /locus_tag="JQH51_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY6076661.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4695..6341
                     /locus_tag="JQH51_002345"
     CDS             4695..6341
                     /locus_tag="JQH51_002345"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY6076662.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6464..7093
                     /locus_tag="JQH51_002346"
     CDS             6464..7093
                     /locus_tag="JQH51_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY6076663.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7232..8641)
                     /gene="melB"
                     /locus_tag="JQH51_002347"
     CDS             complement(7232..8641)
                     /gene="melB"
                     /locus_tag="JQH51_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY6076664.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8756..10111)
                     /gene="melA"
                     /locus_tag="JQH51_002348"
     CDS             complement(8756..10111)
                     /gene="melA"
                     /locus_tag="JQH51_002348"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY6076665.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10394..11302
                     /gene="melR"
                     /locus_tag="JQH51_002349"
     CDS             10394..11302
                     /gene="melR"
                     /locus_tag="JQH51_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY6076666.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11501..13768
                     /gene="adiA"
                     /locus_tag="JQH51_002350"
     CDS             11501..13768
                     /gene="adiA"
                     /locus_tag="JQH51_002350"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY6076667.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14093..14854
                     /locus_tag="JQH51_002351"
     CDS             14093..14854
                     /locus_tag="JQH51_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6076668.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14991..16328
                     /gene="adiC"
                     /locus_tag="JQH51_002352"
     CDS             14991..16328
                     /gene="adiC"
                     /locus_tag="JQH51_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY6076669.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     KPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16432..18075
                     /gene="eptA"
                     /locus_tag="JQH51_002353"
     CDS             16432..18075
                     /gene="eptA"
                     /locus_tag="JQH51_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY6076670.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18072..18740
                     /gene="basR"
                     /locus_tag="JQH51_002354"
     CDS             18072..18740
                     /gene="basR"
                     /locus_tag="JQH51_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY6076671.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18741..19841
                     /gene="pmrB"
                     /locus_tag="JQH51_002355"
     CDS             18741..19841
                     /gene="pmrB"
                     /locus_tag="JQH51_002355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY6076672.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19817..19906)
                     /gene="pmrR"
                     /locus_tag="JQH51_002356"
     CDS             complement(19817..19906)
                     /gene="pmrR"
                     /locus_tag="JQH51_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY6076673.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(20018..21520)
                     /gene="proP"
                     /locus_tag="JQH51_002357"
     CDS             complement(20018..21520)
                     /gene="proP"
                     /locus_tag="JQH51_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY6076674.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21784..22662)
                     /locus_tag="JQH51_002358"
     CDS             complement(21784..22662)
                     /locus_tag="JQH51_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076675.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFDDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22659..24887)
                     /gene="crfC"
                     /locus_tag="JQH51_002359"
     CDS             complement(22659..24887)
                     /gene="crfC"
                     /locus_tag="JQH51_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY6076676.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25290..25625
                     /locus_tag="JQH51_002360"
     CDS             25290..25625
                     /locus_tag="JQH51_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY6076677.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25783..26226
                     /gene="yjdN"
                     /locus_tag="JQH51_002361"
     CDS             25783..26226
                     /gene="yjdN"
                     /locus_tag="JQH51_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY6076678.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26359..27147
                     /gene="phnC"
                     /locus_tag="JQH51_002362"
     CDS             26359..27147
                     /gene="phnC"
                     /locus_tag="JQH51_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY6076679.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27172..28188
                     /gene="phnD"
                     /locus_tag="JQH51_002363"
     CDS             27172..28188
                     /gene="phnD"
                     /locus_tag="JQH51_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6076680.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28294..>28442
                     /locus_tag="JQH51_002364"
     CDS             28294..>28442
                     /locus_tag="JQH51_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HAY6076681.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7305 a         7424 c         6946 g         6767 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctacacga ctttcgtttg agattaaata
       61 acttgccgtt gccacgtacg caattgccag aatgctgaca tatcgcagtg ttgaaataga
      121 catcgatctt ttcgccggta taacagcgat aacccccgtc cagtagcgcc tgatccatga
      181 ccttccctct ctcatcagta tgattatcgc tataagcata gccccggaat cttcatacag
      241 cactggcagt ttccgttgcc agagtctctt cccctatata gccaatcatt tttactccct
      301 ttgaattacc cgcctcatca gagataatgc ttaagaaatt tgtcacacaa ggaagctgat
      361 gagacattca ttgccctacc acatattacg caaacgtccg atgaaattga gtaccactgt
      421 gatcttaatg gtcagcgcgg tactgttctc ggtgctattg gtggtgcatc tgatttactt
      481 ctcgcaaatc agtgatatga cgcgagacgg gctggccaac aaggcactgg cagtggcgcg
      541 taccctcgcc gactcgccgg aaatccgtca gggcttgcag aaaaaaccgc aggagagtgg
      601 catccaggcc atcgcggaag ccgtgcgcaa acgcaacgat ctgctgttta ttgtcgttac
      661 cgatatgcaa agtcttcgct actcacatcc tgaagcccag cgtattggtc agccatttaa
      721 aggtgatgac atccttaatg cgctgaatgg cgaagaaaat gtcgctatca atcgcggttt
      781 tctggcgcag gctttacgcg tatttacccc catctacgat gaaaatcata aacaaattgg
      841 cgtggtggcg atcggccttg agttaagccg cgtgacccaa cagatcaatg acagtcgctg
      901 gagcattatc tggtcggtat tatttggcat gctggtcgga ctgattggca cctgcattct
      961 ggttaaggta ctgaaaaaaa tccttttcgg cctggaaccc tacgaaatct ccacgctgtt
     1021 tgagcaacgc caggccatgt tgcagtctat caaagaaggc gtcgttgccg tggacgatcg
     1081 cggcgaggtc acgctgatca acgatgccgc acaagaattg ctgaattacc gtaagtcgca
     1141 ggacgatgag aaactgtcga cgctcagcca ctcatggtca caggtggtag atgtctcgga
     1201 agtgttacgc gacggtacgc cgcgccgcga cgaagagatt acgattaaag accggctatt
     1261 actgatcaac accgttccgg tgcgcagtaa tggcgttatc atcggtgcca tttcaacctt
     1321 cagggacaaa actgaagttc gtaaactgat gcagcgactc gacggtctgg tcaactatgc
     1381 tgacgcactt cgtgaacgat cccacgaatt tatgaataaa ttgcatgtga ttctcggatt
     1441 attgcatctg aagagttata agcagttgga agattacatt ctcaaaacag ccaataacta
     1501 tcaggaagag attggctctc tgctgggtaa gatcaaatct ccggttatcg ctggtttttt
     1561 aatcagcaag attaaccgcg cgaccgattt aggccatacg ctgattttaa acagtgaaag
     1621 ccagctgcca gacagcggca gtgaggacca ggtcgcgacg ctgattacca cattgggaaa
     1681 tctgatagaa aacgcgctgg aagcattagg gccggaaccc ggtggcgaaa ttagcgtaac
     1741 attgcactac cgtcacggct ggctgcactg cgaagttaac gatgatggac cggggatcgc
     1801 acccgacaaa atcgatcaca tttttgacaa aggtgtctcg acaaaaggaa gcgagcgagg
     1861 cgtcggttta gcacttgtca aacaacaggt agaaaatctc ggcggcagca tcgccgtgga
     1921 atcggaaccg gggattttca cacaattttt tgtccagata ccctgggacg gggagaggtc
     1981 gaacagatga tcaatgtatt aattatcgat gacgacgcaa tggtcgcgga gctgaatcgc
     2041 cgatacgtag cacaaatccc aggctttcaa tgctgtggaa cagcctcgac gctggagaaa
     2101 gccaaagaga ttatcttcaa tagcgatgcg cctatcgacc tgatattgct cgatatctat
     2161 atgcaaaaag agaacgggct cgatttactg cctgtcctgc ataacgcgcg ttgcaaaagt
     2221 gatgtgattg tcatctcctc cgcagccgat gcggcaacca ttaaagattc gctgcattac
     2281 ggtgtcgtgg attacctgat caaacccttc caggcttccc gctttgaaga ggcgctcacc
     2341 ggctggcggc aaaagaaaat ggcgctggaa aaacatcagt attatgacca ggccgagctt
     2401 gatcagctaa tccacggcag cagctccaac gaacaggatc ctcgtcgctt gccaaaaggc
     2461 ttaacgccgc agacgttacg cacgctgtgc cagtggattg acgcgcatca ggactatgag
     2521 ttttcaaccg acgagctggc taacgaggtt aacatctcgc gtgtttcctg ccgaaaatac
     2581 ctcatctggc tggtcaactg ccacatcttg ttcaccagta tccattatgg cgtcacgggg
     2641 cgtccggttt accgttaccg tattcaggca gaacactact cactgctgaa acaatattgc
     2701 caataacgga tcgctggtta tctgtaagta ataattatta ccgtaatgca aaatacccct
     2761 ggcacaagtt acatcgttaa tcagtaaaac ttgtgccaga tcaaatataa ttatccctcc
     2821 atcatcgtta aaaattaata tctcttcaga tgaacggtgt ttttaatttc aaaacgataa
     2881 caaaagttaa ttaactatta tgtcacccgc attatgtgta tttttaccca caaatgggta
     2941 gatcagatta atctataaac ctaatgatat ctgccctgag aacaaaaaat agaccgataa
     3001 atatcaataa gataacagca aacaaaacat taacatctgc gcagtacaaa ctataaaccc
     3061 atcgccagag agtttttctc tctgaaaaag ccgcttatca cagtgcataa atttgccgct
     3121 gctttaatca gccaatattc actgtgaggt atttgctaaa gccggtaacg accaaacgga
     3181 tatttagtca ggctctgaaa acagttcata caaaacagaa cgtgactgtg atctattcag
     3241 caaaaattta aataggatta tcgcgagggt tcacacatgt tatttactat ccaacttatc
     3301 ataatactaa tatgtctgtt ttatggtgcc agaaagggtg gtatcgcgct gggtttatta
     3361 ggcggtatcg gtctggtcat tctggtcttc gtcttccacc ttcagccagg taaaccacca
     3421 gttgatgtca tgctggttat cattgcggtg gtggcggcat cggcgacctt gcaagcttcg
     3481 ggcggtcttg atgtcatgct gcaaattgcc gagaagctgc tgcgccgcaa cccgaaatat
     3541 gtctcaattg tcgcgccgtt tgtgacctgt acactgacca ttctttgcgg tacgggtcat
     3601 gtggtttaca ccattctgcc gatcatctac gacgtcgcca ttaagaacaa catccgtccg
     3661 gaacgtccga tggcggcaag ttctatcggt gcacagatgg ggattatcgc cagtccggtg
     3721 tcggttgcgg tcgtgtctct ggttgcgatg ctgggtaatg tcacctttga tggtcgccat
     3781 cttgagttcc tcgatctgct ggcaatcacc attccatcga cgttaatcgg tatcctggcg
     3841 atcggtatct tcagctggtt ccgcggtaaa gatctggata aagacgaaga gttccagaaa
     3901 ttcatctccg taccggaaaa ccgtgagtat gtttacggtg ataccgcgac gctgctggat
     3961 aaaaaactgc cgaaaagcaa ctggctggca atgtggattt tcctcggggc aatcgctgta
     4021 gtcgcccttc ttggtgctga ttcggacctg cgtccatcct tcggcggcaa accgctgtcg
     4081 atggtactgg ttattcagat gtttatgctg ctgaccgggg cgctgattat tatcctgacc
     4141 aaaaccaatc ccgcgtctat ctcaaaaaac gaagtcttcc gttccggtat gatcgccatc
     4201 gtggcggtgt acggtatcgc atggatggca gaaaccatgt tcggtgcgca tatgtctgaa
     4261 attcagggcg tactgggtga aatggtgaaa gagtatccgt gggcgtatgc cattgttctg
     4321 ctgctggttt ccaagtttgt aaactctcag gctgcggcgc tggcggcgat tgttccggtc
     4381 gcgctggcga tcggcgttga tccggcatac atcgtggctt cagcaccggc ttgctacggt
     4441 tattacatcc tgccgactta tccgagcgat ctggcagcga ttcagtttga ccgttccggc
     4501 accacccaca tcggtcgctt cgtcatcaac cacagtttta ttctgccggg gttgattggt
     4561 gtgagcgtat cgtgcgtctt cggctggatc ttcgccgcga tgtacgggtt cttataaatg
     4621 cactttgcgt gccgcccgtg actacgcggc acgccatttt cgaataacaa atacagagtt
     4681 acaggctgga agctatgtca aacaaaccct ttatctacca ggcacctttc ccgatgggga
     4741 aagacaatac cgaatactat ctactcactt ccgattacgt tagcgttgcc gacttcgacg
     4801 gcgaaaccat cctgaaagtg gaaccagaag ccctgaccct gctggcgcag caagcctttc
     4861 acgacgcttc tttcatgcta cgtccggcac accagaaaca ggttgcggct attcttcacg
     4921 atccagaagc cagcgaaaac gacaagtacg tggcactgca attcttaaga aactccgaaa
     4981 tcgccgccaa aggcgtgctg ccgacctgcc aggataccgg caccgcgatc atcgtcggta
     5041 aaaaaggcca gcgcgtgtgg accggtggcg gtgatgaaga agcgctgtcg aaaggcgtct
     5101 ataacaccta tatcgaagat aacctgcgct actcgcaaaa cgcggcgctg gacatgtaca
     5161 aagaggtcaa caccggcact aacctgcctg cgcaaatcga cctgtacgcg gtagatggcg
     5221 atgagtacaa attcctttgc gttgcgaaag gcggcggctc tgccaacaaa acgtatctct
     5281 accaggaaac caaagccctg ctgactcccg gcaaactgaa aaacttcctc gtcgagaaaa
     5341 tgcgtaccct cggtactgca gcctgcccgc cgtaccatat cgcgtttgtg attggcggta
     5401 cgtctgcgga aaacaacctg aaaaccgtca aattagcaag cgctcactat tacgatgaac
     5461 tgccgacgga agggaacgaa catggtcagg cgttccgcga tgtccagctg gaacaggaac
     5521 tgctggaaga ggcccagaaa ctcggtcttg gcgcgcagtt tggcggtaaa tacttcgcgc
     5581 acgacattcg cgttatccgt ctgccacgtc acggcgcatc ctgcccggta ggcatgggcg
     5641 tctcctgctc cgctgaccgt aacattaaag cgaaaatcaa ccgcgaaggt atctggatcg
     5701 aaaaactgga acacaatcta ggtcagtaca ttccacaaga actgcgtcag gccggtgaag
     5761 gcgaagcggt gaaggtcgac cttaaccgcc cgatgaaaga gatcctcgcc cagctttcgc
     5821 aatatccggt ttccactcgt ttgtcgctca ctggcaccat tattgtgggt cgagatattg
     5881 cacacgccaa gctgaaagag ctgattgacg ccggtaaaga acttccgcag tacatcaaag
     5941 atcacccgat ctactacgcg ggtccggcga aaacccctgc cggttatcca tcaggttcac
     6001 ttggcccaac caccgcaggt cgtatggact cctatgtgga tctgctgcaa tcccacggcg
     6061 gcagcatgat catgctggcg aaaggcaacc gcagccagca ggttaccgac gcgtgtcata
     6121 aacacggcgg cttctacctc ggtagcatcg gcggtccggc ggcggtactg gcgcagcaga
     6181 gcatcaagca tctggaatgc gtcgcttatc cggagctggg tatggaagct atctggaaaa
     6241 tcgaagtaga agatttcccg gcgtttatcc tggtcgatga caaaggtaac gacttcttcc
     6301 agcaaatcgt caacaaacag tgcgcgaact gcactaagta acctcttcgg cccagcgcct
     6361 ggcagcatgc tgccaggtga tccccctggg ccacctcttt tgcgattgta atttcatgct
     6421 tgctggtgaa tagtcagtat tttccctgac ttgcgaactc accatgaccc gcacactcaa
     6481 gccgttaatt cttaacactg gcgcactggc gctaacgtta atcctgattt ataccggcat
     6541 ttcggcccat gacaaactca cctggctgct ggaagtgaca ccggtgatta tcgtcgtgcc
     6601 gctactactt gccaccgcca gacgttaccc gttaacgctg ctgctctata cgctcatctt
     6661 ctttcacgcc atcatcctga tggtaggcgg gcagtacacc tacgcgaaag tccccgttgg
     6721 ttttgaggtg caggaatggc tggggttgag tcgtaatccg tatgacaagc tggggcactt
     6781 tttccagggg ctggtgcctg cactggtggc acgagaaatt ctcgttcgcg ggatgtacgt
     6841 gcgcggacgt aaaatggtgg cgtttctggt ctgttgcgtg gcgctggcga taagcgcgat
     6901 gtatgaatta atcgagtggt gggcggcact ggcgatgggc cagggagcgg atgattttct
     6961 cggtacccag ggcgaccagt gggacacgca atccgatatg ttctgcgcgc tgcttggcgc
     7021 attaacgaca gtgatattgc tcgctcgctt tcattgccgc cagctacggc gctatggctt
     7081 gataactgga taacaacgtg cactttgttg atgccggatg cggcgtgaat tccttatccg
     7141 gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata aacatatcgc
     7201 atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc actgcaccga
     7261 ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc agcagatgaa
     7321 tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag agaatcagcg
     7381 ttaccatgaa aaacagagtc ggtagagcaa tcataataaa ctgcatacct aatagggctt
     7441 gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc gcaatgaaaa
     7501 aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa gcgatgcttt
     7561 cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc atgatgacct
     7621 gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc acaatgagga
     7681 cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt aacaccggaa
     7741 gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag cgtgggaaga
     7801 atactaacgt caccaggtta gcagctcccg catacgacag ataatagggg aacaaatccg
     7861 catcaccgat aacatatgag aaataataga tagcaaatcc ggtaataatg ttgctggcta
     7921 cattataagc aagagccata cccaagaggc atgaaagctg atcgttttta taaataagcg
     7981 caacgatggc tttaagtgtc agatggcttc cttcagcaga cgattgattg tctgacgaaa
     8041 agacttcatg cacattgcgc agagtgatga tggttgaaac aataaaaaag gcgatcaata
     8101 ccagagtgaa catctgaaag ccaaatcccc gatcgccacc gccgacataa ttaacaaatg
     8161 gtagcgtcac acctgccgta acaaagcctg ccagactggc aaaaaaacgc ggataaggaa
     8221 ccagttgttc gcgttcacgt ttatcgagcg tgatggttgg aaccagcgac cagaagggaa
     8281 tatccataat ggtgtaagtc atgccccaga ggatgtaggt cacgcaaaca aagacaatct
     8341 gagtagtacc ttcaaacaga tgcgcactaa agaggagaaa taagattaca gagtttgcca
     8401 acgtaccgat caggatccag ggtttgaact taccccatcg cgatcgcgta gcatttacaa
     8461 tccatcccat aatcggatcg ttaatagcat cccagatcct cgccaccaga aacaaagtac
     8521 cgaccaaacc cacagacagc ccgacgacat cggtgtagta atacatgagg tacatataca
     8581 caatgccgat cgcaaaatcc ttcccgaacg ctccaaatcc ataactgagt tttgtagtca
     8641 ttgaaatgct catagggtat cgggtcgctg ttaccaacgc ccgccttctg ttattggtat
     8701 cgtcagaggt gtgcgccgga ataaatcccc cggcgcagta gcgtttagtc gcgttttaac
     8761 ggtgcaacca gcttggcagc cagtcgccgt gggcggcaat caggtcgtca acaagagcat
     8821 atatttcgtc aatgcccagc acggcggcag tatgcgggtc catcatcgcg gcgtggtaaa
     8881 cacggtcgcg attttccgta agaatagctt cggtcagcag cgtctgtacg ttgatgttgg
     8941 tttgcatcag ggcggccaga tgcgaaggta gcgtaccgac tttggtcggc tgaatgccat
     9001 tagcatcaac cagacaggct acttccacgc aacatccttg tggcaggtta tcaatcaaac
     9061 catcgttacg gacgttgccg taaatcacac tcggctcgcc agtccagata gcgttcatga
     9121 ttgtgctggc atattcccgt gacggtttaa tatcaatccg ggaggcgttt ttatactcct
     9181 ccagctcttt atgccagttc gccagctgct cgacgcagcg tttcgggtac tcatccagcg
     9241 gtactttata acgctcaatc aaatcctcac gacctagctt aataaaccac ggcgtatatt
     9301 cggcaaaatg ttctgatgat tccgtgacga agtagcccag ctttttgaac atttcgtagc
     9361 gcacaatatt ctggcagcga gtattaccat gaatatttgg cttcggtgcc tgccctgctt
     9421 cataagccgc cagcagttcc gggtagagat tcacgtaact gccgtcggcg gttttgcgct
     9481 ccagctccag gtaaaacgcc atatggttga tacctgcgca acggtaacgc agcgtagccg
     9541 ggtcgatatt gaggtcacgc gccagctctt ccgccgttcc ctgcaccgaa tggcacagcc
     9601 cgacctgttt gatatacgga tagcgggcat acatcgccca ggtattcatc gccattgggt
     9661 taacatagtt gagcatggtg gcatcggggc agacttccgt catgttctcg caaatttgcc
     9721 acagatgcgg aatggtacgt agcgcgcgca taataccgcc cggccccaac gtatcggcaa
     9781 tggtttgttc cagaccatgc cgcttacaga cctcgaaatc agtcaccgtg caaggttcat
     9841 aaccgccaat ctgaaatgcc accacgacaa aatcggcatc ctctaaggct tctttctgtt
     9901 gggtgtggca ggttattttg ccgctggccc ctgctgaatc catcagctta cgcaccacaa
     9961 tatgcgactc ttccaggcgg gtggggtcaa tgtccatcag ggcaatatgc gccgttttca
    10021 gcgcctcgcg atggaacaca tcaccaagaa tatttttaac gaaaatcgtc gaaccagcgc
    10081 cgataaatgt aattttgggt gcagacatca tgcagatctc ctggcttgct tgaataactt
    10141 catcatggca ggcgaatctg cagaaaacct ccggcttcca ggttgagcat tcccataaac
    10201 tcagatcctg cgtgaagcag cagtaaatct gagtttatgg gaataaatta cctgaaaagt
    10261 agagttttcg gcctcatgat gctcggacag tcccctcgcc cctctgggaa gagggtgaaa
    10321 accgtgctcc cactcgcagt catcctccct cactcctgcc ataattctga tattctagga
    10381 aagagagcca tccatgaata cagatacgtt tatgtgcagc agcgacgaaa aacagacccg
    10441 cagcccgctg tcgctgtatt cggaatacca gcggatggag attgagtttc gcgcaccgca
    10501 tatcatgccc accagccact ggcatggtca ggtcgaagtg aatgtgcctt tcgatggcga
    10561 tgtggaatac ctgatcaaca atgaaaaagt gaatatcaat cagggtcata tcacgctgtt
    10621 ctgggcctgt acaccgcacc aactaacaga taccggaacc tgtcagagca tggcgatttt
    10681 taatctgccg atgcatctgt ttctctcctg gccgctggat aaagacctga ttaaccacgt
    10741 cactcacggc atggtgatca aatcacttgc cacacagcaa cttagcccgt ttgaagtacg
    10801 ccgctggcag caggaattaa acagtccgaa tgagcaaatt cgccagctcg ccattgatga
    10861 aattggcctg atgctcaagc gatttagcct ctctagctgg gaaccgattc tggtcaataa
    10921 aacctcgcgc acacacaaaa acagcgtctc gcgccatgcg caattttatg ttagccagat
    10981 gctgggcttt attgccgaaa actatgatca ggcgctgacc atcaacgatg tggctgagca
    11041 cgtcaaactt aacgccaact atgcaatggg gatattccag cgggtcatgc aattgacgat
    11101 gaaacagtac attaccgcga tgcgcatcaa ccacgttcgc gcgttactga gcgataccga
    11161 taaaagtatt ctcgatattg ccctgacggc aggctttcgt tcaagtagtc gtttttacag
    11221 cacgttcggc aaatatgtcg gcatgtcgcc gcaacaatac cgcaaactta gccaacagcg
    11281 ccgccagacg tttcccggct aaagacgata tcagtatcag ccaaaaaaat agtttagccg
    11341 tcgataataa gcagtgaaaa taatttaaaa taatcacata acgtattgtt tataaaacta
    11401 aatatttcaa aaatgtttgt ttttcacgcg ctttacagcc cgaaaaggcc ggaagatact
    11461 tgcccgcaac gaagattcct tcataaccgg gtaagcaatg atgaaagtat taattgttga
    11521 aagcgagttt ctccatcaag acacctgggt cggtaacgcc gttgagcgtc tggcagatgc
    11581 tttaagccag caaaatgtta ccgtgattaa atccacctcc tttgatgatg gttttgccat
    11641 tctctcttca aacgaagcca ttgactgcct gatgttcagc tatcaaatgg agcatccgga
    11701 cgaacatcaa aacgtcagac aattgatcgg taagcttcat gagcgccaac aaaacgtgcc
    11761 ggtcttcctg ttgggcgatc gggaaaaagc cctcgccgca atggaccggg atctgctgga
    11821 gcttgtcgat gaattcgcct ggattctgga agataccgcc gactttatcg ccggacgcgc
    11881 cgttgccgcg atgacccgct accgccagca gctgttgccg ccactgttca gcgcgctgat
    11941 gaaatatagt gacatccatg aatattcctg ggcagcgcca ggccaccagg gcggcgttgg
    12001 ttttaccaaa acacccgccg gacgtttcta ccatgactac tatggtgaaa atctgttccg
    12061 taccgacatg ggcatcgaac gaacttccct cggttctttg cttgaccata ctggcgcatt
    12121 tggcgaaagc gaaaaatatg ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt
    12181 cgtcggtact tccggctcta accgcaccat catgcaggct tgcatgaccg ataacgatgt
    12241 cgtggtcgtt gaccgtaact gccataaatc catcgaacaa ggtttgatgc tgacaggcgc
    12301 gaaaccggtc tatatggtgc caagccgcaa ccgctacggc attatcgggc caatctatcc
    12361 gcaggaaatg caacctgaaa ccttgcaaaa gaaaatcagt gaaagcccgc tgaccaaaga
    12421 caaagccggg caaaaaccgt cttactgcgt ggtaaccaac tgcacctatg acggcgtgtg
    12481 ttataacgct aaagaagcgc aggatctgct ggaaaaaacc tccgatcgtc tgcactttga
    12541 cgaagcctgg tacggctatg cacgtttcaa cccgatctat gccgatcact atgccatgcg
    12601 cggcgaacct ggcgatcaca acggtcctac cgttttcgcc acccactcca tccacaaact
    12661 gctgaatgcg ctgtcacagg cttcttatat tcatgtacgt gaaggtcgtg gggcgattaa
    12721 cttctcccgc ttcaaccagg cctacatgat gcatgccacc acctccccgc tgtatgccat
    12781 ctgcgcatcc aacgacgtgg cggtgtcgat gatggacggc aacagcggcc tgtcactgac
    12841 acaggaagtg attgacgaag cggttgattt ccgtcaggcg atggcgcggc tatataaaga
    12901 gttcaccgct gacggtagct ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc
    12961 acaaaccggc aaaacctatg actttgctga cgcaccaacc aaactgctga ccaccgttca
    13021 ggactgctgg gtaatgcatc cgggcgaaag ctggcacggc ttcaaagata ttccggataa
    13081 ctggagtatg ctcgacccaa ttaaagtcag catccttgct ccgggaatgg gtgaagatgg
    13141 tgaactggaa gaaaccggtg ttccggcggc gctggtcact gcctggcttg gtcgccacgg
    13201 cattgtgcct acccgcacca ctgacttcca aattatgttc ctgttctcta tgggcgtaac
    13261 ccgtgggaaa tgggggactc tggttaacac cctttgctcc ttcaaacacc actatgacgc
    13321 caacacaccg ctggcgcagg tgatgccgga acttgttgaa caatatcctg acacttacgc
    13381 gaacatgggg attcacgatc tgggtgacac catgtttgcc tggctgaaag aaaacaaccc
    13441 tggcgcacgg ttgaacgaag cctattccgg cctgccggtg gcggaaatca ccccgcgtga
    13501 agcgtacaac gcgattgtcg acaacaatgt cgaactggta tccattgaaa atctgccagg
    13561 acgcatcgcg gcaaactcag ttatcccgta tccgccagga atcccgatgc tgctgtctgg
    13621 tgaaaacttc ggcgataaaa acagtccgca agtaagttat ttacgctcgc tgcaatcctg
    13681 ggaccaccat ttccctagat ttgaacacga aactgaaggg actgaaatta ttgacggtat
    13741 ttaccacgtt atgtgcgtga aagcgtaacc actattccgc tgaaggcgta attgtttaaa
    13801 taacattacg ccgcctggcc ttaggccttt tgagtatggc aacgttttca taaaaattgc
    13861 tgcaaacaaa aatgtcatac tttttgcgcg gccccacccc acgctttttt ttgcctgtta
    13921 tttatcctgt aaaaaaatat gtacatgaga aattactata aaaatttgta atattagtaa
    13981 aactcgttat ttttatgcat gtttatattc atcatgcaat tatataacca tttcccggta
    14041 tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat gtatgaggat
    14101 ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga taagggtgaa
    14161 tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt gtgaaaataa
    14221 tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc acgacatcat
    14281 caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat ggcaacgtag
    14341 cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg ttgccgcgca
    14401 acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg cattattatt
    14461 cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga tgtatatgtt
    14521 acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc acaaagactg
    14581 gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa agaaaaagtt
    14641 gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc gttatgccgt
    14701 aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct gcggctacaa
    14761 cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc cgctgcatta
    14821 tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga agagctatat
    14881 taataacggc atcagcgata acccggtcga taataattca actatcgaat gcaggcgtat
    14941 gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt atgtcttcgg
    15001 atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg gggaatatta
    15061 tggggtcagg tgtttttctg ttacctgcaa acctggcctc cactggcggg attgccattt
    15121 atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac gccaaaatgt
    15181 cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc tttggcccgt
    15241 ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc aatatcgcca
    15301 tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa gatccattgg
    15361 tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac attgtcggtc
    15421 cgaaaatgat cacccgcgtg caggccgttg ccactgtgct ggcgctgatc cccatcgtcg
    15481 ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg gcatggaacg
    15541 tcagcggcct gggcaccttc ggtgcgattc aaagtaccct taacgttacg ctgtggtcgt
    15601 tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaaaccg aaacgcaatg
    15661 tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgtg ctctccacca
    15721 ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcctcg ccattcggtg
    15781 atgccgcacg gatggcgttg ggtgacaccg ctggggccat tgtttccttc tgcgcagctg
    15841 cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg gcgaaagccg
    15901 ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg ggtacgccag
    15961 tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc agcatttcac
    16021 caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt acactggtgc
    16081 catatcttta cacctgtgcg gcgttactgc tgctcggcca cggtcacttt ggtaaagcac
    16141 gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatttgg gccgtggtgg
    16201 ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc accgccatgt
    16261 atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca ccaataagca
    16321 aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact taaggttggc
    16381 ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt gatgttgaag
    16441 cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc cgctttttat
    16501 atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct gccgctggat
    16561 tcgctgcata acgtactggt tttcttgtcg atgccggttg tcgctttcag cgtgattaat
    16621 attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg cctgtttatt
    16681 ctgattggcg cggctgcaca atatttcata atgacttacg gcatcgtcat cgaccgctcg
    16741 atgattgcca atattattga taccactcca gcagaaagtt atgcgctgat gacaccgcaa
    16801 atgttattaa cgctgggatt cagcggcgtg cttgctgagc tgattgcctg ctggctaaaa
    16861 atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa tattctggtt
    16921 tctgtactgc tgattttgct ggtcgccgca ctgttttata aagactacgc ctcgttgttc
    16981 cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt tgccagctgg
    17041 tcatggtact cccatcagcg actggcaaat ctgccgctgg tgcgaattgg tgaagacgcg
    17101 caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct gattgtcggc
    17161 gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac taacccgcgg
    17221 ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac ggcaacggca
    17281 gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc actacaaaga agagctggca
    17341 cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt gctgtggaat
    17401 gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa cgtcaccgcg
    17461 ctgaatctgc ctgatcagtg catcaacggc gaatgctatg acgaagtgct gttccacggg
    17521 ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca caccatcggc
    17581 agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt taccccaacc
    17641 tgcgacacca atgagatcca gacctgtacc aaagagcaac tggtgaacac ttacgacaac
    17701 acgctggttt acgtcgacta tattgttgat aaagcgatta atctgcttaa agaacatcag
    17761 gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt aggtgaaaat
    17821 ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa acaggtgcca
    17881 atgctgctgt ggctgtcgga ggattatcaa aagcggtatc aggttgacca gaactgcctg
    17941 caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac gctattggga
    18001 ttaactggcg ttgagacgaa gtattaccag gccgcggatg atattctgca aacttgcagg
    18061 agagtgagta aatgaaaatt ctgattgttg aagacgatac gctgttattg cagggactga
    18121 ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg cggatggcgg
    18181 aacaaagcct tgaggacggt cattacagcc tggtggtact ggatttaggg ttacccgacg
    18241 aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg ccggtactga
    18301 tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc ggtgccgatg
    18361 actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc gccctgctac
    18421 gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg ctgaatatgg
    18481 gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa gaatatgctc
    18541 tgctgtcacg gttaatgctc aaagcaggca gtccggtaca tcgggaaatt ctctacaacg
    18601 acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat atccacaatc
    18661 tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc tatatgctgg
    18721 tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct gcgccgacct atatcgctgc
    18781 gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg atcagcgtct
    18841 tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg ctacgcgaca
    18901 atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc agcctgattg
    18961 tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg gtacgccgca
    19021 tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc gacaacttaa
    19081 cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg gcgttgaacg
    19141 atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc gctgacgtcg
    19201 cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg ctggcgaaaa
    19261 cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg gagagcgtct
    19321 cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat tatcaacatg
    19381 taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc atgctcgacc
    19441 agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt cagggtgatg
    19501 caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt tacagcccgc
    19561 aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg gcagttgaag
    19621 atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg tttgtacgta
    19681 tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc attacacagt
    19741 tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg cgggcctggg
    19801 tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg ctgaccacca
    19861 gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca ctctcctccg
    19921 ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt tcaattggcc
    19981 tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt cgcggatgtt
    20041 gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca atattatcga
    20101 ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc tgtatatctg
    20161 acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg gttacgccgg
    20221 tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc agattctgcg
    20281 agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc accgaaatat
    20341 taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct ggcaaggtag
    20401 aagccataac gcccataaag cagttaagga tcaccgccag catcagtaac ccggcaaaaa
    20461 tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac acaaacagtg
    20521 caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc aagcccatca
    20581 ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc ccgtggtctt
    20641 cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac atgtagtaag
    20701 tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag tatttagtgg
    20761 caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg ccctgttcca
    20821 gtttatcaac atgctgctgg aacgccggag tttcttccag cgcatggcgc aggtaaagcc
    20881 cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag tcgaggaagt
    20941 tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc cgcacccagc acaaacccgg
    21001 caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca ggggagtatt
    21061 ccgcaacaaa tatcgacgcc ccggtatatt caccaccgac cgagaaacct tgtgccatct
    21121 tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac ggtattaagc
    21181 caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc ttctggcgac
    21241 catatttatc gcccaacata ccaaagaaga gtccgccaag cggtcgaatc agaaagggaa
    21301 cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc gggaaaaaaa
    21361 ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat tccattgcat
    21421 tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata atggtgacat
    21481 cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc gcagagttgg
    21541 cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga gcgggaattg
    21601 caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg ttaaggaatg
    21661 tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac cctaccagtg
    21721 tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga tgtaactgat
    21781 tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccacca aacgctgcgc
    21841 tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca ctgaccagca
    21901 aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat cagcatgatc
    21961 cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac gcgtagtcgg
    22021 tgccgcattg atactctctg ccagatgttg tgtcgctttc tcgcgcaaat cacccagcaa
    22081 caacaccacc gcttgcgggc ttcccagacg gcaggcatcg tcaaatgcac cccagcgcaa
    22141 ctcctccaga acgataaaac agcgcccagg caacgaccag gcgttatacg ccccagcacg
    22201 ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc ctcgttgcgt
    22261 taacgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg cggcaatttg
    22321 ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca gcagcgccag
    22381 atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg ccagtttttc
    22441 caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg tccatgtctg
    22501 aagctgcaca cgcgaaagaa gttcctgcat cagtcgttca cgaaactgct gctgatgggc
    22561 ttgaggatgg cgggcatcat cgccctgcgc cagatccact aaaaagcgcg gatgaaccga
    22621 ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc tgccgtgaaa
    22681 agtgtttgaa tatcatcgcg taacagctga gcgtcggtgt aaatccagct gtgagtcatc
    22741 acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg ttgacgcaga
    22801 gccagacttt cgcgtaaacg cgtctgtaat tctgtcaaac atgaagcgaa ctcagcgaaa
    22861 aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac tttagccatt
    22921 tgttggcaat agagatcgac ttctgcgctt aatgctcgtt gcaacacact gtaatcaacc
    22981 gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa ccagcgcgaa
    23041 aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc ctggcgcgaa
    23101 atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg aaaaacagga
    23161 aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg ttgctcaatg
    23221 gggcgaataa catcggttaa cacccggcaa agggtggatt ccatctcggc aaaacgcagc
    23281 gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca gcgggtacga
    23341 atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc attcaccgca
    23401 tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat ttcatgttta
    23461 atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc ttcgacctga
    23521 tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg aaaatccagg
    23581 tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg cagagcatac
    23641 aatgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc gaacagcgaa
    23701 tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag atcggcatga
    23761 cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg ttgctctggc
    23821 gctggtaact taccgttgtt ggctaactca tagcgcgccc gattcgccag gtagccccac
    23881 atcgacgaca ccgggaatat ctgctgtggc gtaatacagc ctttcatcag cgtcccggaa
    23941 atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc gaacttattg
    24001 accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg gacctcttca
    24061 tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga ggcgcgtgcc
    24121 agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg cccgggggta
    24181 tccagtaacg tcaactgacc gggataactt tccagccccg ccagatggac aaactccact
    24241 tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc gacgtccagc
    24301 gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat aggctgggca
    24361 cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag cgcacgcata
    24421 tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg caggcgctgt
    24481 tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag taccggttcc
    24541 ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg attacgatta
    24601 ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc tttcatggta
    24661 ccgacaattg ccagcaccat ttccagtcgg gaaattttac acaactcatt attcagcatc
    24721 gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg cgcgtcgtct
    24781 ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga aagctgtaac
    24841 aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga aaattcttcc
    24901 ttaaagcaaa ttttgttact tttatttagc cagattgttt ttgagttctg ttttcggctt
    24961 ttataattac tgcaagaaat aattttatat ttagtgtgtt gtttttttat cagaataaat
    25021 aacgtcttct gatgcgttta aaacgtcaga aagataaaaa tatcatgtga ataaaaaaaa
    25081 gaacaagtag agcattaaca ttatcttaaa taataaatag aggcaaaaag attattttct
    25141 ttttgcgttt cctttcaaat gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa
    25201 actcagggat tttgtgtata attgcggcct ttttcggcaa tctgccgttt tttggcgctt
    25261 ttgccctgct gacttttgag gaaatccaca tgtcattacc acactgccca aaatgcaact
    25321 ccgaatacac ttacgaagat aacggcatgt acatctgccc ggaatgtgcc tacgaatgga
    25381 acgacgcaga acctgcacag gaaagcgacg agctgatcgt taaagatgct aacggcaatc
    25441 tgctggctga cggcgacagc gttaccatca ttaaagatct gaaggtgaaa ggtagctctt
    25501 cgatgctgaa aattggcacc aaagtgaaaa acatccgcct ggttgaaggc gaccataaca
    25561 tcgattgcaa aatcgacggt tttggtccga tgaaactgaa atctgagttt gtgaaaaaga
    25621 actgattgta ttgtgatcgg taagccggat aaggcgctcg cgccgcatcc ggcaacgatg
    25681 ccgactgcct gatgcgacgc ttgcgcgtct tatcaggcag cctctcatct gtataaattt
    25741 cgaactacac ttaactggct tctcttaact gaggtcacca tcatgccgtt aagtccctac
    25801 ctctcttttg ccggtaactg ttccgacgcg attgcctatt atcaacgtac gttgggcgcg
    25861 gaactgctct ataaaatcag cttcggcgaa atgccgaaat cagcgcagga cagcgccgag
    25921 aactgccctt ccggaatgca atttcccgat accgccatcg ctcatgccaa cgtgcgcatt
    25981 gccggaagcg acatcatgat gagcgatgct ataccgtcag gaaaagccag ctactccggc
    26041 tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt tgataacctt
    26101 gccgctaacg gaaaaatcga aatggcctgg caggaaactt tctgggcgca tggctttggc
    26161 aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg
    26221 caataacccg ccgggaggcc cgccctcccg cactgtcatc gaattcccgt taactcttca
    26281 tctgttagtc acttttaatt aaccaaatcg tcacaataat ccgccacgat ggagccactt
    26341 ttttagggag gctgcatcat gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc
    26401 aatcagcatc aagcgctgca tgcggttgat ctgaacattc atcacagtga aatggtggct
    26461 ctgcttgggc cgtcgggttc cggcaaatcc acccttttac gtcatttaag cggtttgatt
    26521 accggcgata aatccgccgg tagccatatc gagctactgg gccgcactgt ccagcgcgaa
    26581 ggccgcctgg cccgcgatat ccgcaaaagc cgcgccaaca ccggctacat ctttcaacaa
    26641 ttcaacctgg tgaaccgcct gagcgtactg gagaacgtgc tgattggcgc gctcggcagc
    26701 acgccgttct ggcgcacctg ttttagctgg ttcaccggcg agcagaaaca gcgcgcgtta
    26761 caggcgctga cccgcgttgg catggtgcat tttgcccatc agcgcgtttc caccctctcc
    26821 ggcggccagc agcaacgtgt ggcgattgcc cgtgcgctga tgcagcaggc gaaagtgatt
    26881 ctggccgatg aacccatcgc ctcgctggac ccggaatcag cgcgcatcgt gatggacacc
    26941 ctgcgcgaca tcaaccagaa cgacggcatc accgtggtcg tcacgctgca tcaggtggat
    27001 tacgccctgc gctactgcga acgcatcgtc gccctgcgtc aggggcacgt cttctacgac
    27061 ggcagcagcc aactgtttga taacgaacgt tttgaccatc tctaccgcag cattaaccgc
    27121 atcgaagaga acgcgaaagc tgcctgatat ccccatcatt gaggaaaacg aatgaacgct
    27181 aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct gttaaccccg
    27241 gcacacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac ggaatcacag
    27301 caaaacctga aaccgcaatg gacgccgttt ttacaggata tggagaagaa actgggcgtg
    27361 aaggtcaacg ccttctttgc cccggactac gcgggcatta tccaggggat gcgcttcaat
    27421 aaagtggata ttgcctggta cggcaacctg tcggcgatgg aagcggtgga tcgcgccaat
    27481 ggccaggtct tcgcccagac ggtcgcggcg gatggatcgc cgggttactg gagcgtgttg
    27541 atcgtcaaca aagacagccc gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat
    27601 ctcaccttcg gcaatggcga tcctaactcc acctctggct tcctcgtccc cggttactac
    27661 gtcttcgcta aaaacaatat ctccgccagc gacttcaagc gcaccgtcaa cgccgggcat
    27721 gaaaccaacg cgctggccgt cgccaacaag caagtggatg ttgccaccaa caacaccgaa
    27781 aacctcgaca agctgaaaac ctccgcgccg gagaagctta aagagctgaa ggtgatctgg
    27841 aaatcgccgc tgatcctagg cgatccgatc gtctggcgca agaatctgtc cgaaactacc
    27901 aaagacaaga tctacgactt ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg
    27961 ctggaacgcc tgggctgggc gccgttccgc gcttccagcg acctgcaact ggtgccgatt
    28021 cgccagctcg cactgtttaa agagatacaa agcgtgaaag gcaataaagg gctgaatgag
    28081 caggacaagc tggcgaaaac caccgagatt caggcgcagc tggatgacct ggaccgcctg
    28141 aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg tgcagtaaat cgtaggtcgg
    28201 ataagacgcc ccggcgtcgc atccgacaat gtgtaggcgt tgatgccgga tgcggtgcaa
    28261 gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat cgccccaccc
    28321 aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgacgtgct ggtcgtctcg
    28381 tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc
    28441 tt
//
LOCUS       DADLEE010000053        28420 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000053 DADLEE010000000
VERSION     DADLEE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.053"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            563..1333
                     /locus_tag="JQH51_002365"
     CDS             563..1333
                     /locus_tag="JQH51_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY6076682.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="JQH51_002366"
     CDS             1324..1797
                     /locus_tag="JQH51_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY6076683.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="JQH51_002367"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="JQH51_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY6076684.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="JQH51_002368"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="JQH51_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY6076685.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="JQH51_002369"
     CDS             3232..3726
                     /locus_tag="JQH51_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY6076686.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="JQH51_002370"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="JQH51_002370"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY6076687.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /locus_tag="JQH51_002371"
                     /pseudo
     CDS             complement(6395..7417)
                     /locus_tag="JQH51_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
     gene            7556..8470
                     /locus_tag="JQH51_002372"
     CDS             7556..8470
                     /locus_tag="JQH51_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6076688.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="JQH51_002373"
     CDS             8685..10046
                     /locus_tag="JQH51_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076689.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="JQH51_002374"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="JQH51_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY6076690.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="JQH51_002375"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="JQH51_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY6076691.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="JQH51_002376"
     CDS             12596..13885
                     /locus_tag="JQH51_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY6076692.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="JQH51_002377"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="JQH51_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY6076693.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="JQH51_002378"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="JQH51_002378"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY6076694.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="JQH51_002379"
     CDS             16211..16591
                     /locus_tag="JQH51_002379"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY6076695.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="JQH51_002380"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="JQH51_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY6076696.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="JQH51_002381"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="JQH51_002381"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY6076697.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="JQH51_002382"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="JQH51_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY6076698.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="JQH51_002383"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="JQH51_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY6076699.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20661..21517
                     /locus_tag="JQH51_002384"
                     /pseudo
     CDS             20661..21517
                     /locus_tag="JQH51_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="JQH51_002385"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="JQH51_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="JQH51_002386"
     CDS             23320..23832
                     /locus_tag="JQH51_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY6076700.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="JQH51_002387"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="JQH51_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY6076701.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="JQH51_002388"
     CDS             26425..26628
                     /locus_tag="JQH51_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY6076702.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="JQH51_002389"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="JQH51_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY6076703.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28420)
                     /locus_tag="JQH51_002390"
     CDS             complement(27664..>28420)
                     /locus_tag="JQH51_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY6076704.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6957 a         7425 c         7126 g         6912 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac acagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gatatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtataa
//
LOCUS       DADLEE010000054        28364 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000054 DADLEE010000000
VERSION     DADLEE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.054"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="JQH51_002391"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="JQH51_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="JQH51_002392"
     CDS             complement(1023..2045)
                     /locus_tag="JQH51_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6076705.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="JQH51_002393"
     CDS             complement(2059..3561)
                     /locus_tag="JQH51_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY6076706.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="JQH51_002394"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="JQH51_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="JQH51_002395"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="JQH51_002395"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY6076707.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="JQH51_002396"
     CDS             complement(5877..6218)
                     /locus_tag="JQH51_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY6076708.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="JQH51_002397"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="JQH51_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY6076709.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="JQH51_002398"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="JQH51_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY6076710.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="JQH51_002399"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="JQH51_002399"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY6076711.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="JQH51_002400"
     CDS             12897..14240
                     /locus_tag="JQH51_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY6076712.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="JQH51_002401"
     CDS             complement(14302..14508)
                     /locus_tag="JQH51_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY6076713.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="JQH51_002402"
     CDS             14833..15387
                     /locus_tag="JQH51_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY6076714.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="JQH51_002403"
     CDS             complement(15450..16388)
                     /locus_tag="JQH51_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076715.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="JQH51_002404"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="JQH51_002404"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY6076716.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="JQH51_002405"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="JQH51_002405"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY6076717.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="JQH51_002406"
     CDS             complement(19599..19979)
                     /locus_tag="JQH51_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY6076718.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="JQH51_002407"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="JQH51_002407"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY6076719.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="JQH51_002408"
     CDS             21037..22002
                     /locus_tag="JQH51_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6076720.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="JQH51_002409"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="JQH51_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY6076721.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="JQH51_002410"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="JQH51_002410"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY6076722.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /locus_tag="JQH51_002411"
     CDS             complement(24537..25157)
                     /locus_tag="JQH51_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6076723.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="JQH51_002412"
     CDS             25376..25921
                     /locus_tag="JQH51_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076724.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="JQH51_002413"
     CDS             complement(25905..26552)
                     /locus_tag="JQH51_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6076725.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="JQH51_002414"
     CDS             26764..27555
                     /locus_tag="JQH51_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY6076726.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28364
                     /locus_tag="JQH51_002415"
     CDS             27565..>28364
                     /locus_tag="JQH51_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY6076727.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6960 a         7551 c         6992 g         6861 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DADLEE010000055        27598 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000055 DADLEE010000000
VERSION     DADLEE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27598
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.055"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            316..1614
                     /gene="kgtP"
                     /locus_tag="JQH51_002416"
     CDS             316..1614
                     /gene="kgtP"
                     /locus_tag="JQH51_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY6076728.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1611..1955)
                     /locus_tag="JQH51_002417"
     CDS             complement(1611..1955)
                     /locus_tag="JQH51_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY6076729.1"
                     /translation="MFFGGHVLRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="JQH51_002418"
     CDS             complement(1980..3335)
                     /gene="pssA"
                     /locus_tag="JQH51_002418"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY6076730.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3449..6109)
                     /gene="pat"
                     /locus_tag="JQH51_002419"
     CDS             complement(3449..6109)
                     /gene="pat"
                     /locus_tag="JQH51_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY6076731.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6141..6839)
                     /locus_tag="JQH51_002420"
     CDS             complement(6141..6839)
                     /locus_tag="JQH51_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY6076732.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="JQH51_002421"
     CDS             complement(6908..7327)
                     /gene="trxC"
                     /locus_tag="JQH51_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY6076733.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7534..8571
                     /locus_tag="JQH51_002422"
     CDS             7534..8571
                     /locus_tag="JQH51_002422"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY6076734.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8619..9308)
                     /gene="ung"
                     /locus_tag="JQH51_002423"
     CDS             complement(8619..9308)
                     /gene="ung"
                     /locus_tag="JQH51_002423"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY6076735.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9613..9996
                     /gene="grcA"
                     /locus_tag="JQH51_002424"
     CDS             9613..9996
                     /gene="grcA"
                     /locus_tag="JQH51_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY6076736.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10052..10639)
                     /gene="eamB"
                     /locus_tag="JQH51_002425"
     CDS             complement(10052..10639)
                     /gene="eamB"
                     /locus_tag="JQH51_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY6076737.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10742..11623
                     /locus_tag="JQH51_002426"
     CDS             10742..11623
                     /locus_tag="JQH51_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6076738.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11658..12992)
                     /gene="srmB"
                     /locus_tag="JQH51_002427"
     CDS             complement(11658..12992)
                     /gene="srmB"
                     /locus_tag="JQH51_002427"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY6076739.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13124..13861
                     /gene="trmN"
                     /locus_tag="JQH51_002428"
     CDS             13124..13861
                     /gene="trmN"
                     /locus_tag="JQH51_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY6076740.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13846..15468)
                     /gene="nadB"
                     /locus_tag="JQH51_002429"
                     /pseudo
     CDS             complement(13846..15468)
                     /gene="nadB"
                     /locus_tag="JQH51_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15724..15879
                     /gene="rseD"
                     /locus_tag="JQH51_002430"
     CDS             15724..15879
                     /gene="rseD"
                     /locus_tag="JQH51_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY6076741.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15876..16451
                     /gene="rpoE"
                     /locus_tag="JQH51_002431"
     CDS             15876..16451
                     /gene="rpoE"
                     /locus_tag="JQH51_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY6076742.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16484..17134
                     /gene="rseA"
                     /locus_tag="JQH51_002432"
     CDS             16484..17134
                     /gene="rseA"
                     /locus_tag="JQH51_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY6076743.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17134..18090
                     /gene="rseB"
                     /locus_tag="JQH51_002433"
     CDS             17134..18090
                     /gene="rseB"
                     /locus_tag="JQH51_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY6076744.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18087..18566
                     /gene="rseC"
                     /locus_tag="JQH51_002434"
     CDS             18087..18566
                     /gene="rseC"
                     /locus_tag="JQH51_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY6076745.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18764..20563
                     /gene="lepA"
                     /locus_tag="JQH51_002435"
     CDS             18764..20563
                     /gene="lepA"
                     /locus_tag="JQH51_002435"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY6076746.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20579..21553
                     /gene="lepB"
                     /locus_tag="JQH51_002436"
     CDS             20579..21553
                     /gene="lepB"
                     /locus_tag="JQH51_002436"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY6076747.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21825..22505
                     /gene="rnc"
                     /locus_tag="JQH51_002437"
     CDS             21825..22505
                     /gene="rnc"
                     /locus_tag="JQH51_002437"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY6076748.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22502..23407
                     /gene="era"
                     /locus_tag="JQH51_002438"
     CDS             22502..23407
                     /gene="era"
                     /locus_tag="JQH51_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY6076749.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23419..24147
                     /gene="recO"
                     /locus_tag="JQH51_002439"
     CDS             23419..24147
                     /gene="recO"
                     /locus_tag="JQH51_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY6076750.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24159..24890
                     /gene="pdxJ"
                     /locus_tag="JQH51_002440"
     CDS             24159..24890
                     /gene="pdxJ"
                     /locus_tag="JQH51_002440"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY6076751.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24890..25270
                     /locus_tag="JQH51_002441"
     CDS             24890..25270
                     /locus_tag="JQH51_002441"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY6076752.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25690..25770
                     /gene="shoB"
                     /locus_tag="JQH51_002442"
     CDS             25690..25770
                     /gene="shoB"
                     /locus_tag="JQH51_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY6076753.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25964..26224)
                     /gene="yfhL"
                     /locus_tag="JQH51_002443"
     CDS             complement(25964..26224)
                     /gene="yfhL"
                     /locus_tag="JQH51_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY6076754.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26439..27538
                     /locus_tag="JQH51_002444"
                     /pseudo
     CDS             26439..27538
                     /locus_tag="JQH51_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6805 a         6863 c         7237 g         6693 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcgttg gtgaaaaaaa tgcctgatgc
       61 gacgctgcgc gtcttatact cccacatatg acagattcag caacggatgc ggtttcccca
      121 acttgcctac ttccatacgt gtcctcctta ccagaaattt atccttaagc tcctcaataa
      181 ccattttcct gctaactaaa ttcatggttt aggttgcata atgatacgca acaaatgtat
      241 aacattttct ttaccaaaag aaataaacaa aagcgaccaa caaaagcatc ggattacggc
      301 aggagacata atggcatggc tgaaagtact gtaacggcag acagcaaact gacaagtagt
      361 gatactcgtc gccgcatttg ggcgattgtg ggggcctctt caggtaatct ggtcgagtgg
      421 ttcgatttct atgtctactc gttctgttca ctctacttcg cccacatttt cttcccttcc
      481 gggaacacga cgactcaact actacaaaca gcaggtgttt ttgctgcggg attcctgatg
      541 cgcccaatag gcggttggct atttggccgc atagccgata aacatggtcg caaaaaatcg
      601 atgctgttat cggtgtgtat gatgtgtttc ggatcgctgg ttatcgcctg cctcccaggt
      661 tatgaaacta taggtacgtg ggctccggca ttattgcttc tcgctcgttt atttcaggga
      721 ttatccgttg gcggagaata tggcaccagc gccacctata tgagtgaagt tgccgttgaa
      781 gggcgcaaag gtttttacgc atcatttcag tatgtgacgt tgatcggcgg acaactgcta
      841 gccctactgg ttgtcgtggt tttacaacac accatggaag acgctgcact cagagagtgg
      901 ggatggcgta ttcctttcgc gttaggagct gtgttagctg ttgtggcgtt gtggttacgt
      961 cgtcaattag atgaaacttc gcaacaagaa acgcgcgctt taaaagaagc tggatctctg
     1021 aaaggattat ggcgcaatcg ccgtgcattc atcatggttc tcggttttac cgctgcgggc
     1081 tccctttgtt tctatacctt caccacttat atgcagaagt atctggtaaa tactgcggga
     1141 atgcatgcca acgtggcgag tggcattatg actgccgcat tgtttgtatt catgcttatt
     1201 caaccactca ttggcgcgct gtcggataag attggtcgcc gtacctcaat gttatgtttc
     1261 ggttcgctgg cagccatttt taccgttcct attctctcag cattgcaaaa cgtttcctcg
     1321 ccttatgccg cttttggtct ggtgatgtgt gccctgctga tagtgagttt ttatacatca
     1381 atcagtggaa tactgaaggc tgagatgttc ccagcacagg ttcgcgcatt aggcgttggt
     1441 ctgtcatatg cggtcgctaa tgctatattt ggtggttcgg cggagtacgt agcgttgtcg
     1501 ctgaaatcaa taggaatgga aacagccttc ttctggtatg tgaccttgat ggccgtggtg
     1561 gcgtttctgg tttctttgat gctacatcgc aaagggaagg ggatgcgtct ttagtgacgg
     1621 gtcagttgcc agacggtata gccggtgctt gcaccggcga catcccaggc caaatccttc
     1681 cagctccagc cgctcccttc ggggcggcta tcccaaagct cttttgacgc ccccaaactg
     1741 acagagaaca tcaatccaaa catggcactg cgatcccggc tcatcccctg atgctgtgaa
     1801 tattcatttc cggcggcgga aagtatcgcc gaggcgataa agtgttgagc tttatcctgc
     1861 ccactccagc tatcgttatc catatggctg cattcagaaa gtaacaatag cgaacagaca
     1921 aaaagaatac gcaaaacgtg gcctccaaaa aacaaacccc gtacaggacg gggttgtgat
     1981 tacaggatgc ggctaattaa tcggtcgatg cggatacggc gcaaacggcg gatgagttta
     2041 cgaaccttca ccggataatc ggcaatactt tgcagatcgc gatagtgctt aacgatggtg
     2101 gtacgctcgc ggatcagctc cagttctttc tctcgctgcg gcgcaagctc cagttgtgga
     2161 tcgtggatca aaatggcgtt ttccagatcc agacgccagg cgcgcgggtt caggttatta
     2221 ccggtgatca acatccactt atcatcaacc cacatccctt tcaggtgata ggtgttgtcg
     2281 tcatctttcc ataaccgaac cactagctgg tcagtattga cgtaatactg caaacggctc
     2341 aggaaacgac gcagattgat ctcatagaga taaggcaatg cgccaattat cttgaaaggt
     2401 tcatcttccg gaatgtagaa gtcgttcgcg gttttatcac caacaataat ttcgaccttt
     2461 ttcccttcgc gcagcaactg gataatattg cgcacaagga ttgctggcag gttgaagtat
     2521 ggcgtacaga tggttagttt ctgctcggca caaggcataa gatggaaaat ggtcttgttc
     2581 aacagactcg atttccccag ccccactagc ggcgttacgg aaagctgatc gttgtcggca
     2641 tcgccctgga aatgataagc ggcatcacgc agctcctggc ggaacagacg aatatcgttc
     2701 ttgatttccg ggctttttgg ccgattaaca tcatccagac gattaacgcc gcggccattc
     2761 ataatattct gtgtaaccca ttcaaacata atgtctgaca tcttacggtt acggatcaga
     2821 tgataacggt cgtagcgata tttatcgtgt tgatgcaggt aaacatcgtt caggctggca
     2881 ccgctataaa gtacgctatc gtcgatgata aagcctttaa agtgcagaac accaagagct
     2941 tcacgggtat tgattggaac gccataaacc ggaacatcta cgcccggatt ttcctgcgcc
     3001 atgcggcagt accagtcagc gttagtgtta gatgccgcag cgccaatgcg tccacgttgt
     3061 gcacgatgcc agtcgaccag cacccgcaca tccagttccg gacgctgcct tttagcctca
     3121 tacaacgcgt tcagaatgcc tttgccaccg tcatcctgtt cgagatacag ggcgacaatg
     3181 caaatgcgct gcttcgcgct ggctattttt tccagcagcg tctcccggaa gtcggcggga
     3241 gcgtaaaaga aatcgacatc atcaactgat tgagaaatct tgggtagttg ggcaaggtgt
     3301 tgttgatgtt tattacgctt aaattttgac aacatcacag tgcatttctt ctctgttcat
     3361 tgaagggtcc tctgtgcaat gcagacgaca taagcgggca ataataacac cagtcccgat
     3421 taagtggtca acatttccag taccttactc atgattcctc gcgccgggca agatttagcg
     3481 taagcccaac gatcccctct tcgagctgga tatcaacgtt aaacccgagc ttgcgggcta
     3541 gcgccaccat gccacgattg tttggcatcg taataccatt cagacgttgt agtccgtgat
     3601 ttcgcgtata ggtaatcaac ttttccatta agcgtcgacc taagcctaac cctttgagat
     3661 ccgagcgaac cagtacagca aattcggcat cgatgttatc aggatcggaa atcgcacgcg
     3721 tgacgccgag gatctcttcc gtttgatcaa tacgtcgtac cgctacaaac gccatttccc
     3781 gatcgtagtc gatctgtgtc atgttggcta aatcttcatg ggtaaattcg ttgatctcgc
     3841 taaagtagcg gtaataaaga tcttctttgg tgactcgcga aatgaattgc tgaagttgtg
     3901 gctcatcttc tggcaaaatc gggcggaaca agcagcgttc accgtttttc aattctaccc
     3961 attcttccag ctgatgcgga taagggcgca ctgccagccg actctcgtta tcgccttcaa
     4021 acggcgagat atccagcgtg acatccagcg cggtaaattc actgccagaa gccagcaaag
     4081 gatgaatatc cagacgctga atttccgggc aatcgacaat caagttggaa acctgcacca
     4141 gaagctggct caagcctgca acatccaatg ggcgtagcgc actgcgcgca cgaatctttt
     4201 tacttttgat cccctgaata accagatagc gggccaggtt catgttcagc ggcggcagtg
     4261 cgacgacggc ttgatcttca ggacgccact ccacaccgcc ttcacccagc atgatcaacg
     4321 gcccgaaaac cggatcgtgc tcaaccacaa cccgcaactc ctgagcgcca gcacggttag
     4381 ccatactttg caccaacagg ccgtggaccc gcgcctgtgg ccaggccatt tttacgcgat
     4441 cgaaaatagc gttcgccgct tgctggactt cattggctgt acgcaggtaa agcatgacgc
     4501 cctgaacttc cgatttatgt ggaatatccg gcgaacgcaa tttcagcgcc accggataac
     4561 caatctgttc ggcaatatgc accgcttcgg tgctatcgct ggcaatccag gttgggagcg
     4621 tgttcatgcc atacgcttgc aggatgggct gaacttcatg ggtatcgagc gacgtagccc
     4681 cttcggcaat cgcctgttgc aacagaagat gcgcttctgc ggtattggaa gtcagattgc
     4741 tgggcaacgc cggcgtttcg cgtagttgct tctgattacg ccggtactcc accatatgca
     4801 taaaagcagt gatggttcct tccggggtac ggtaggtcgg cagcccggct tcgctgaata
     4861 aacgtcgtgc ctcttgcgag gagtgctcgc cgcaccagtt cgtcagcaaa gagacatatt
     4921 tgctgcgggg atgatgcttt accgcttcaa ttaatacttg cgcgctttct gttgcgggag
     4981 cagcggcgct gggcgaatga ataaccatca gtgcatcaaa atcctggctg tggagcagaa
     5041 tgtccagcgt tttgacataa tgctcgctgc tggcgtcatc gcgcagatcg agcgggttag
     5101 agactgccac atgttctggc aatgcatcgc gcaatttctg gcaggtttct tcgcttagcg
     5161 ttgccagctt gccattgcgt gaccataagg catccagcgc cagcgcggca ggcgcagcac
     5221 cgttgctgat aatcatcaac cggtcgccac gcagcgggcg catatggcta agggtttcca
     5281 ccgccgaaaa cagttcgtgg gtatcctgta cccgcaacaa accggcacgc tgaatagccg
     5341 catcccatgc cgggtccatt cctgccgtgg tgttgagcag tcgctgtgcc gccgggctac
     5401 gtccgctttt aatcaccaga atcggtttat tacgcgaggc actacgggcc gccgaaacaa
     5461 agcgtcgcgc gtcgcttaac tgttcgagat agagcaggat ggcgctggtt ttactgtcgc
     5521 gcgccagata gtcgagcaat tcatcaacgt cgatatctag gctgtcgccg agcgcaataa
     5581 agtaggaaaa gcccatctca cgctgttgcg cccagtcgag gatggtgttg gagacggcag
     5641 ccgattgcga aataaacgcc agcttgccgc gtttaatcgg cacaggcgaa aagctggcat
     5701 tcagaccttg ccagggagcc agtaaaccca gactgtttgg tccaagcagg cgcatgttat
     5761 ggcgcagggc gcaggcgcgg agatcttcgt gttgcgatgc cggggcggaa agaataatgc
     5821 aggttttaca gcctttctcg ccgagctctt ccagaagagc aagattacgg ctggcattgg
     5881 tacataaaac cgcaaggtcg ggtgtaaagg gcaagctggc aatatccggc caggccaaca
     5941 cacccaacac cgctttccag gctggcgtca ccgggagtac cggtccgtta aagcctcccg
     6001 ccagcaggtt acgcatcatc aggtaacctg cgcgattggg tttcatcgac gcgccaatta
     6061 ccgctatcga ttttggtcgc agtagtgctt ccagtcctcg ctgactcata ccggtctccc
     6121 gcagaagtga ccgaatgatt ttaaacgctt tctaactgtt ctgctgtgat gctacccaga
     6181 tgttgcgttt ttcctgccag atagcgtgtt ttaaagcggg taaaatgctc gcctaaccct
     6241 gctgccgccc cggtatcgcc ggccatatct aacagtgcga tggctacctc ggcagtacaa
     6301 tattggcctg cagcctgggc ttcacgcagg cgataggcag aaagccggga aagatcgacg
     6361 gaaatgacgg gaagattatc cagatacgga cttttacgaa acatcttgcg agcttccggc
     6421 caggtaccat cgagcatgat aaacagcggt ggcttaccgg caggtggtgt gaagatcact
     6481 tcccgttgct catcagcata cgaggcggga aagaccacca ttggctgata atccgggttt
     6541 tgtaccagat ccagcaaatc ctgcgagggt tcggtacgcg accattgaaa cgcaacggta
     6601 tcaggcaaaa tatcagcaat cagacgcccg gtattactgg gcttcattgg ctcggtgtcg
     6661 aacatcagca aacagaagcg actttttgct tgtgctgggg taattgtcga acagagacat
     6721 aatttctctg gcaaaagaca gcgttggcag cgacgaacgc gattaccgcg ggcaagaaaa
     6781 ggacgtgttg cgcgcgcaat acgctcggcg cgtaactgga gaacagcgtt ttcggtcata
     6841 agagagcgtc gaaaaaacgc cattgtcgca gaggagaaaa cggggcacaa gatgcgcccc
     6901 ggtaagatta aagagattcg ttcagccagc tatcgaacgg cgctttcggt actgcgccat
     6961 taagcatgtc gacaacctga ccgtttttga aaatcatgat cgtcggtata ctacgaattc
     7021 caaagcgact gctcaattca cgttcagctt cggtattcac tttcacaaag cgcactttac
     7081 cgctacgctc ttgcgcgaca tcttcaaaaa ttggtgcgaa attacggcag gggccgcacc
     7141 acggtgccca gaagtcgatc accacaggta gatcatcctt cagcaatttg tcgagtgttt
     7201 caccggtcgc attaatcacc tctccgtcaa acaagtcgtg accgcagcgt ccgcattttg
     7261 ccgcatcttc gatccgatcg tcgggaatgc gattgatggc ctgacaatgg gtacaaacgg
     7321 tattcataac taacctcggg atgagtaagg agacttatcg gcaacgacgc cggtatgttt
     7381 ctaatatgtt acatattatc ggggacattg ttccagacaa caatctgttt tattcaatag
     7441 gtatgatagt cgcaggcttt gctacgaaat catggcgaac cgcgcggact tcgggtaatc
     7501 tgcgcgcttc gcgcagcgct ggtggagaaa agcatgaacg atgaaatgaa aggtaaaagc
     7561 ggcaaggtca aagtgatgta tgtccgcagt gatgatgatt ctgataaacg tacccacaac
     7621 ccgcgtaccg ggaaaggggg cgggcgtcca ggaaaatctc gtgctgatgg tggccgtcgc
     7681 cccgcccgcg atgacaaaca gagtcagccc cgtgaccgca agtgggaaga ttcgccgtgg
     7741 cgcacggttt cccgcgcgcc gggtgatgag acgccggaaa aggccgatca cggtggcatc
     7801 agtggtaaaa gttttattga tccggaagtg ttgcgtcgtc agcgtgcgga agaaacccgc
     7861 gtctacggcg aaaacgcctg tcaggcactg ttccagagcc gtccggaagc gattgttcgc
     7921 gcctggttta tccagagtgt aacgccgcgt tttaaagaag ccttgcgctg gatggcagca
     7981 aaccgcaaag cgtaccatgt ggtggatgaa gcggaattga caaaagcgtc aggtacggaa
     8041 catcacggcg gcgtttgctt cctgatcaaa aagcgtaacg gtacaaccgt gcagcagtgg
     8101 gtaatccagg caggcgcgca ggattgcgtt ctggcactgg aaaacgaatc taacccgcat
     8161 aacctgggtg gcatgatgcg cagctgcgcg cacttcggcg tgaaaggtgt cgtggtgcag
     8221 gatgcggcac tgctggagtc gggggcggct atccgtaccg cagaaggcgg cgcagagcac
     8281 gttcagccga ttactggcga caacattgtt aacgtgctgg atgatttccg tcaggcaggt
     8341 tacaccgtag tgaccacttc cagcgagcag ggtaaaccgc tgttcaaaac cagtctgcca
     8401 gcgaaaatgg tactggtgct gggtcaggaa tatgaagggt taccggatgc cgcacgcgat
     8461 ccgaacgatc tgcgcgtgaa gattgacggt acgggcaacg ttgccgggct gaatatctct
     8521 gtcgcaaccg gcgttttgct tggtgaatgg tggcgtcaga ataaagcctg ataaatcagc
     8581 cgggtggcaa ctctgccatc cggcatttcc ccgcaaattt actcacactc tgccggtaat
     8641 actggcatcc agtcaatcgg cgtctcgcca cgttgttcca gccactgatt tgccagcaca
     8701 aaatggttgc agccaaagaa tccacgatgc gccgaaagcg gcgacggatg cggtgctttc
     8761 agtacatgat ggcgttgctt atctataatc gcccctttct tttgcgcatg tgatccccac
     8821 aacaaaaaca ccacgccttc gcggtgctgg ttaatcaggc tgatgacttt atcagtaaac
     8881 gtctcccaac cgaggctggc gtgggaatgc gcctgacctg cgcgaaccgt caacacagta
     8941 ttgagcagca gaacgccctg acgcgcccag ctttcgagat agccatgatt agggcgggtg
     9001 aagcccggaa tagtattttc cagctcttta tacatattca ataatgacgg aggaatggca
     9061 atgccgggac gaacggaaaa tgccagacca tgcgcctgtc ccggtccgtg ataaggatcc
     9121 tggccgagaa tcaccacttt aacgtcaccc aactcggtaa agcggaacgc gttaaagaca
     9181 tctttttgtg gtggatagat agtgacgccg gactgccgct cgctggcgac ggtctgaagg
     9241 gtattaagaa aataaggttg ttgcttctct tcagccagca cgtcatgcca ggttaattcg
     9301 ttagccatct tgctctcctg cgaatcttca atccgcctag cttaactgct tctttctaca
     9361 gaacaaaatc atgctcattt tttagactta aacgagtgca aaaaaatgta aattggtaaa
     9421 atttcacacc cgcataagtt gatgtaaaac aataaaatcc atttacgacc acaatcgatg
     9481 tgtggttttt gttgatttaa atcaaagaat caagggtggt tgaggagtat atatacactc
     9541 aagcaacaat ggttttacca attggccgcg acaggctgaa caaatcaaat aattttgccg
     9601 gggaggcatc acatgattac aggtatccag attactaaag ccgctaacgc cgatctgctg
     9661 aactctttct ggctgctgga cagcgaaaaa ggcgaagcac gttgcatcgt tgcaaaagca
     9721 ggttatgcag aagatgaagt ggttgcagta agcaaactgg gtgacattga ataccgtgaa
     9781 gttccagtag aagtgaaacc agaagttcgc gttgaaggtg gtcaacacct gaacgttaac
     9841 gttctgcgtc gtgaaactct ggaagatgca gttaagcatc cggaaaaata tccgcagctg
     9901 accatccgtg tatccggtta tgcagttcgc tttaactctc tgactccgga acagcagcgc
     9961 gacgttatcg ctcgtacctt tactgaaagc ctgtaaggct taagtagcaa gttactaagc
    10021 ggaagagggc gacctcttcc gctttttttc gttaatagaa aatgcgtacc gcgcaataga
    10081 ccagcaacag ggcaagcacg atatttaact ggcgaccata ctggcgaaac aatcgctgaa
    10141 acagatgccc cgccagcgcc cagcacacat tgccaaacgt cccaatcatc gccagcaaaa
    10201 cgctgacgcc aactacccag cttagcgcct gtgtttgcgg cagaacaaac gtcgacagtg
    10261 ccgtaacacc gtacaaaatg attttgacgt tcacaaactg caaagcaaag ctggcccaaa
    10321 agctgattgg ttttgcctga agtccgtctt cctttgttgg gctggtggcg attttccacg
    10381 ccagccagac aatatatgcc gcccccgccc aactcaaaag gtgtaccgct gccgggtcaa
    10441 tcactgccag tgaaaatgaa atgcccgcac acagtaacat cacaatcaaa aatcccagac
    10501 tcatccctgc cagcacgcgg gtactttgac gaaatccatg cgacgtagca gagctaaggg
    10561 cgagaatatt gttcggtcct ggcgtcatag cggtaatcag ggtgtaagtc caaaaagcac
    10621 ttaaaaggat cggtgtcact tgttatctcc tctgttttgg tagacagtac ccgacacgga
    10681 acatttaaaa aaatgaatgt ttttatattc taaattcgat attttcgata ggtttggggt
    10741 tatggatctg cgccgtttta ttacgcttaa aaccgtggtg gaagagggtt cctttttgcg
    10801 agcttcgcaa aaattgtgct gtacacaatc gacggtgact tttcatattc agcagcttga
    10861 gcaggagttt tcagtccagt tatttgagaa aattggccga cggatgtgcc ttacccgcga
    10921 aggaaaaaag ttgctgccgc acatttatga acttacccga gtgatggata cgcttcgtga
    10981 ggctgccaaa aaggagtcag atccggacgg ggaactacgc gttgtttcgg gcgaaacgtt
    11041 gctctcttac cgaatgccgc aggtgttgca gcgttttcgg caacgcgcac caaaagtgcg
    11101 tttatcgttg caggcgctga actgttacgt gatccgtgat gcgctactga atgatgaggc
    11161 cgatgtcggc gtcttttatc gcgtagggaa tgatgatgcc ctgaaccgac gagagttggg
    11221 tgaacaatcg ctggtgctgg tggcttcacc acaaattgca gatgtcgatt ttactgagcc
    11281 gggaagacat aacgcctgta gctttattat caacgaaccg caatgtgtct tccggcagat
    11341 atttgagagc acgctgcgac agcggcggat cacggtggaa aacaccattg agcttataag
    11401 tatcgaaagc atcaagcgtt gtgtcgcggc gaatatcggt gtcagttact tgccccactt
    11461 tgcggtggaa aaagagattg agagcggaga attaattgaa ttgccttttg gcgaacagtc
    11521 gcagaccatt acggcaatgt gcgcacatta tgctggaaaa gcggttagcc cggcgatgca
    11581 catttttatt cagtgtgttg aagagtgttt tgtggttgcg taaaaaaaca atgccgggct
    11641 taaacccggc attgagatta ctcttctgtc gtttgcggtg gcacgcccgt tccgctcggt
    11701 ttacggcgct taccaatatt tttggtgtcg cgatggcgtt ttttcacccg cggcttctct
    11761 ttctctttag cttttttctt ctcggcgcgt tttgccagta ctttcttcga tggcttgccg
    11821 gtctgcttct cgcttggcgc acgcgttttc gggcgtaact catcaataac gcgagcttta
    11881 atcggctctt caatgtaacg gccaaccttg cccagcagca aatggtcatg ggcttccacc
    11941 agcgaaattg cggtgccttt acgaccggcg cgcgcggtac gtccgatacg atgcaaataa
    12001 gtatcgccac tgcgcggcat atcgaagtta aagacgtggc tgacgtcagg aatgtcgata
    12061 ccgcgcgcgg caacatcggt ggcaaccagt acgtttacgc ggccttcggt caggcgttta
    12121 attgcttcgt tacgcttgcc ttgtaccatc tcaccttcga gatagcagtt gttgatgccc
    12181 gcttcgcgca gccagtttgc cagctcatgc acacgttcac gcttacgcac aaacacgatt
    12241 gagcgagtcg cttcaggctg ttttaacagg tgcaccagca atgcggtttt atgttccagg
    12301 tcatcggcgc ggtaatacca ctgatgaatt tttttgcgct cacgggtgga cggattggca
    12361 gaaacttcca ccggatcttc cagcagacgc tcggcaaagt cctgaatcgc atcgccttcc
    12421 agcgttgccg aaaagagcag ggtctgttta cgccagcgag tttcgccagc aatatgttcg
    12481 atatcctgag cgaagcccat atccagcata cggtctgctt cgtcgaggat cagcgtttca
    12541 accgcgcggc aatcgaagtt ctcttctttt atgtattgca gcagacgtcc ggtcgtggcg
    12601 accacgatgt cctgattttc gctgaacact tccgcgtggt tcatataggc tacgccgccg
    12661 gtgatggtgg cgatatccag atgcgtatgt ttcgccagtt cgcgggcatg atcggccacc
    12721 tgcatcgcca gctcgcgagt tggggtgagg atcaaaatac gcggcggacc ggatttctta
    12781 cgtgggaaat cgagcaggtg ctgcaacgct ggcagcagat acgccgccgt tttaccggtg
    12841 cctgtcggcg cagaaccgag tacatcacgg ccatcgagcg caggcggaat ggcggcagcc
    12901 tgaatggcgg tcgggcgagt gaaaccttta tcctggaggg cttccagcag gctttcgtcg
    12961 agttcaagtt cggaaaaagt cgttacagtc atgttctacc tctgtgtggg gcgctgatta
    13021 tagacgttac ggctgcaatc ttcatctgtt tgtatggata tcgcttctcg ggtattgttc
    13081 tcacccggtg ctatcgccgt ttttcctgca aggttttatt ttcatgtcac agtctacatc
    13141 cgtgcttcgt cgtaatggat ttacttttaa acagtttttt gttgctcacg atcgctgtgc
    13201 gatgaaagtg ggaacggatg gcattttgct gggcgcatgg gcaccggtgg ctggggtgaa
    13261 acgttgcctt gatatcggcg cgggtagcgg gttgctggca ctgatgctgg cgcagcgaac
    13321 cagtgacagc gtgataattg atgccgttga gctggaaagt gaagctgccg cgcaggcgca
    13381 ggaaaatatc aaccagtccc cgtgggcaga gcggattaac gtccatacgg cggatattca
    13441 gcagtggatc acacagcaga cagtacgctt cgatttaatc atcagtaacc caccttacta
    13501 tcagcaggga gtagagtgcg cgacacctca acgggaacag gctcgctata ccactacgct
    13561 tgatcaccca tcgctgctga cctgtgcggc ggagtgcatt accgaagagg gatttttctg
    13621 cgtggtgctg ccagagcaga ttggtaatgg ttttactgaa ctggcattaa gcatgggctg
    13681 gcatttacgt ttgcggacgg atgtggcgga aaacgaagcg cgactgccgc atcgggtgct
    13741 gctggcattc tccccgcagg cgggagaatg ctttagcgat cgcttagtca ttcgtgggcc
    13801 agaccagaac tattccgaag cgtatacggc gctgacccag gctttttatc tgtttatgta
    13861 atgattgccg ggggaaagga tcgatggacc ggaatgcgtg agcagttccg ggtaatccag
    13921 cgtaaaatgc agcccccggc tttctttgcg catcatcgcg cagcgaacaa tcaactcggc
    13981 aacctgtacc agattacgca gctccagcaa attatttgag acgcggaaat gggcgtaata
    14041 ttcgtctatt tcttgttgga gcatggttat ccgccgcagg gcgcgttcca ggcgcttcgt
    14101 tgtgcgcaca atgccaacgt aatcccacat aaacagccgt agctcgtgcc agttatgctg
    14161 aattactacc cgttcgtcag ggttctcaac gcggctttca tcccacggcg gtaacgtact
    14221 gacgccgtgg gcataaggca tacgtctggt gatatcttct gccgccgacc agccatagac
    14281 cagacactcc agcaatgaat tcgaggccat gcggttagcg ccgtgtaagc cggtataact
    14341 cacctcgcca atggcataca agccctcgac gtccgtacgc ccatgatcat caaccattac
    14401 accacctcag gtatagtgtg cagcaggcac aatcggtacc ggttcttgtg tgagatcaat
    14461 ccccagcccg agcaattttt cataaatcat cgggaaatgc tggcgaataa aatcggcggg
    14521 cttatggctg atgtcgagga acatacaatc tgcgccgagg cgtttcattt catggtcaat
    14581 ggcgcgggcg acaatatcgc gcggggccag ttcgccgcgc tcatcaaaat cgggcataaa
    14641 acgcgtgcca tccgggcgct tgagataagc gccttcgccg cgcagtgctt ccgttaacag
    14701 gaaattgcgt gcctgtgggt gatacagtgc ggtagggtgg aactgattaa attcgagatt
    14761 ggcaacccga cagcccgcgc gccacgccat agcaatgcca tcgccagaag aaatatccgg
    14821 attggtggtg tactgataca ccttcgacgc accgccggtt gccagtacca ctgcttttgc
    14881 gtggcaggtt tccaccgttt ctttatttcg gttccatacc cacgcgccta caacccgtcg
    14941 cgtgcccggc aggccaattt tgtcagagac aatcagatca actgcgttgc tgcgctccag
    15001 cacacgaata ttcggatggt tctgcgcctt gctcaccagc gtggattgta cttctctacc
    15061 ggtggcgtcg gcggcatgaa gaatacgacg gtgactatgt ccaccttcac gggtcagatg
    15121 gtagctttct tcgccattcg gttgaacgtg ggtatcaaac aaaaccccct ggtcaattaa
    15181 ccatggcaca caggaacgag cattgctggc gacaaattca actgcatggt gatcgcaaat
    15241 accagccccg gcaatcaatg tgtcttccac atgcgagtca atgctgtcag tttcatcaaa
    15301 cacggcggca ataccgccct gggcataaaa tgttgaacct tccgttaccg ggcctttact
    15361 tagaacgatg acctgatgct ggtcagccag gcgtagcgcc agtgaaagtc cggctgcgcc
    15421 gctaccaata atcaacacgt cacatgaatg ttcagggaga gtattcattt tctttgttta
    15481 atttactaaa cattgtttgg tcggcatagc atcatgttgt gcgaataaac acctgctatt
    15541 ttaatatttg ttacagtggc taaacacgtt gacgcagggc ggcgagaaaa agagaagtta
    15601 ctggctggtg gaggattagg tggtgaaata aaaaggccgt tgggttactc ttcaggcagt
    15661 taaatgggca tttctacaca gataatgcga tgttcagatt ctgtagactt ataatgatag
    15721 ataatgatcc gtctacagca tgacaaacaa aaacagatgc gttacggaac tttacaaaaa
    15781 cgagacactc taaccctttg cttgctcaaa ttgcagctaa tggagtggcg tttcgatagc
    15841 gcgtggaaat ttggtttggg gagactttac ctcggatgag cgagcagtta acggaccagg
    15901 tcctggttga acgggtccag aagggagatc agaaagcctt taacttactg gtagtgcgct
    15961 atcagcataa agtggcgagt ctggtttccc gctatgtgcc gtcgggtgat gttcccgatg
    16021 tggtacaaga agcttttatt aaagcctatc gtgcgctgga ttcgttccgg ggagatagcg
    16081 ctttttatac atggctgtat cggattgctg taaatacagc gaaaaattac ctggttgctc
    16141 aggggcgtcg tccaccttcc agtgatgtgg atgccattga agctgaaaac ttcgaaagtg
    16201 gcggcgcgtt gaaagaaatt tcgaaccctg agaacttaat gttgtcagaa gaactgagac
    16261 agatagtttt ccgaactatt gagtccctcc cggaagattt gcgcatggca ataaccttgc
    16321 gggagctgga tggcctgagc tatgaagaga tagccgctat catggattgt ccggtaggta
    16381 cggtgcgttc acgtatcttc cgagcgaggg aagctattga taacaaagtt caaccgctta
    16441 tcaggcgttg acgatagcgg gatactggat aagggtatta ggcatgcaga aagaacaact
    16501 ttccgcttta atggatggcg aaacgctgga tagtgagctg cttaacgaac tggctcataa
    16561 cccagaaatg cagaaaacct gggaaagcta tcacttaatc cgtgattcaa tgcggggtga
    16621 tactcccgag gtgctccatt tcgatatctc ttcacgcgtg atggccgcca ttgaagaaga
    16681 gccagtacgt caatcggcga cattgatccc ggaagcccag cctgcgccgc atcaatggca
    16741 gaaaatgcca ttctggcaga aagtacgtcc gtgggcggca cagcttaccc aaatgggcgt
    16801 agccgcatgc gtatcgcttg cagttatcgt tggcgtccag cactataatg gacaatctga
    16861 aacgtcccag cagcccgaaa cgccggtatt taatacactg ccgatgatgg gtaaagccag
    16921 cccggtaagc ctgggagtac cttctgaagc gaccgcaaac aatggtcaac agcagcaggt
    16981 acaggagcag cgtcgtcgca ttaatgcaat gttgcaggat tacgaactgc aacgccgact
    17041 ccactctgaa cagcttcagt ttgagcaggc gcaaacccag caagccgctg tacaggtgcc
    17101 aggaattcaa actttaggaa cgcaatcgca gtaatgaagc aactttggtt tgccatgtca
    17161 ttagtgacag gtagcctgtt attctctgct aacgcctcgg ccactcccgc gtccggggcg
    17221 ttattacagc agatgaacct ggccagtcag tcactgaatt acgagctgtc attcatcagc
    17281 atcaataaac agggtgttga gtctctgcgt tatcgacatg cacgcctcga taaccgtcct
    17341 cttgcacaat tgttgcaaat ggatggcccg cgccgggaag tggtacagcg cggcaatgaa
    17401 atcagctatt ttgaaccggg acttgaaccg ttcacgctta atggcgatta cattgttgat
    17461 tctctgccat cgcttatcta taccgatttc aaacgccttt ctccttacta cgactttatc
    17521 tccgtcgggc gcacgcgtat tgctgatcgt ctttgcgaag tcattcgcgt ggttgcccga
    17581 gatggtacac gctacagcta catcgtgtgg atggacaccg aatcgaaatt accgatgcgg
    17641 gttgatctcc ttgatcgcga tggtgaaacg ctggaacaat ttcgcgtgat tgcttttaac
    17701 gtcaatcagg atatcagcag cagtatgcag acgctggcga aggcaaattt gccgccgttg
    17761 ctttctgttc ctgtaggtga aaaagctaaa ttcagctgga cgccaacctg gttgccacag
    17821 ggttttagcg aagtttccag tagtcgacgt ccgctaccga cgatggacaa catgcctatc
    17881 gaatcacgtc tctattccga cggattattc agcttctcgg taaacgttaa ccgcgctacg
    17941 ccatcgagca ccgatcagat gttgcgcacc ggacgcagaa ccgtcagtac aagcgtacgt
    18001 gataacgccg aaatcaccat tgtcggtgaa ctgccgccgc aaacggcgaa acgcattgcc
    18061 gagaatatta agttcggggc agcgcaatga tcaaagagtg ggctaccgtc gtctcctggc
    18121 aaaacgggca ggcgctggtc agttgtgatg ttaaagcttc atgcagcagc tgcgcttcac
    18181 gcgccggttg cggtagccgc gtgttgaata aacttggccc gcaaaccacg cataccattg
    18241 tcgtaccctg tgatgagccg ttagtgccgg ggcaaaaagt ggagttgggg atcgccgaag
    18301 gcagcctgct tagctccgca ttactggttt atatgtcgcc gctggtggga ttattcctca
    18361 tcgcttcgct atttcagcta ctctttgctt ctgacgtcgc agcattatgt ggtgcggtcc
    18421 tcggcggtat tggcgggttc ctgattgcgc gcggctactc gcgtaagttt gctgcccggg
    18481 cagaatggca accgatcatc ttaagcgtgg cattgccgcc tggccttgtg cgatttgaaa
    18541 catcttcgga agacgcgagc cagtgatttc actggctttg ttgcattttg cataacaaat
    18601 gcgaggacgt ttccagatac cacaatccat acggtatgaa catttcctcg cctcaatgtt
    18661 gtagtgtaga atgcggcgtt tctattaata cagacgttaa gctcagaaca gcgacttcta
    18721 aaagcctggt taaccgggca ttaaggcaca ataatcatac tttatgaaga atatacgtaa
    18781 cttttcgatc atagctcaca ttgaccacgg taaatcgacg ctgtctgacc gtattatcca
    18841 gatctgcggt ggcctgtctg accgtgaaat ggaggcgcag gttctcgatt ccatggatct
    18901 tgagcgtgag cgtggcatta ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc
    18961 tgacggcgaa acctatcagc ttaactttat cgacaccccg ggccacgtag acttctccta
    19021 tgaagtttcc cgttcgctgg ctgcctgtga aggtgcattg ctggtggtcg acgccgggca
    19081 gggcgtagaa gcgcaaaccc tggcaaactg ctacaccgcc atggaaatgg atctcgaagt
    19141 ggtgccggta ctgaacaaga ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga
    19201 aattgaagat atcgtcggca tcgacgccac cgatgcagtg cgctgttcag cgaaaaccgg
    19261 cgttggcgtg caggacgttc tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga
    19321 tccggaaggc ccgttgcagg cactaattat cgactcctgg ttcgacaact acctgggcgt
    19381 tgtttcactt atccgtatta aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat
    19441 gagtaccggg cagacctata acgccgaccg tctgggcatc ttcacgccga aacaggttga
    19501 ccgcactgaa ctgaaatgtg gcgaagtagg ctggctcgta tgtgcgatta aagatatcca
    19561 cggcgctcca gtcggcgata ccttaacgct ggcgcgtaat ccggcagaaa aggcgctgcc
    19621 tggctttaag aaagtcaaac cgcaggtata cgccggtctg ttcccggtaa gttccgacga
    19681 ctatgaagcc ttccgtgacg cgctgggtaa actcagcctg aacgatgcct cactgttcta
    19741 tgagccggaa agctccagcg cgctgggctt tggtttccgc tgcggcttcc ttggcctgct
    19801 gcacatggag atcatccagg agcgtctgga acgtgaatac gatctggatc tgatcaccac
    19861 tgcgccgacc gtagtgtatg aagttgaaac cacgtcaagg gaagttatct acgtcgacag
    19921 cccatccaag ctgcctgcgg taaataacat ttacgaactg cgcgagccga ttgcagagtg
    19981 tcacatgctg ctgccgcagg catatctcgg caacgttatt acgctgtgcg tagaaaaacg
    20041 cggcgtgcag accaatatgg tttaccacgg taatcaggtg gcgctgacgt acgagatccc
    20101 gatggcggaa gtggtgctcg acttcttcga tcgcctgaaa tctacctcgc gtggttatgc
    20161 gtctctggat tacaacttca aacgcttcca ggcgtccgac atggtacgtg tagacgtatt
    20221 aatcaacggt gaacgtgttg atgcgctggc gttgatcacc caccgtgata attcgcaaaa
    20281 ccgcggtcgc gagttggtgg agaagatgaa agatctgatc ccacgccagc agtttgatat
    20341 cgccattcag gcagcgattg gtacgcacat tattgcgcga tccaccgtga aacagctgcg
    20401 taaaaacgta ctggctaaat gttatggcgg cgatatcagc cgtaagaaaa agctgctgca
    20461 gaagcagaaa gaaggtaaga aacgcatgaa gcagatcggt aacgttgagc tgccgcagga
    20521 agcgttcctc gccattctgc acgtcggcaa agacaacaaa taacccttag gagttggcat
    20581 ggcgaatatg tttgccctga ttctggtgat tgccacactg gtgacgggca ttttatggtg
    20641 cgtggataaa ttctttttcg cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc
    20701 tgccggtgac tcactggata aagcaacgtt gaaaaaggtt gcaccgaagc ctggctggct
    20761 ggaaaccgga gcttctgttt ttccggtgct ggctatcgta ttgattgtac gttcgtttat
    20821 ttatgaaccg ttccagatcc cgtcaggttc gatgatgccg actctgttaa tcggtgattt
    20881 tattctggta gagaagtttg cttatggcat taaagatcct atctaccaga aaacgctgat
    20941 cgaaaccggt catccgaaac gcggcgatat cgtggtcttt aaatatccgg aagatccaaa
    21001 gcttgattac atcaagcgcg cggtgggttt accgggcgat aaagtcactt acgatccggt
    21061 ctcaaaagag ctgacgattc aaccgggatg cagttccggc caggcgtgtg aaaacgcgct
    21121 gccggtcacc tactcaaacg tggaaccgag cgatttcgtt cagaccttct cacgccgtaa
    21181 tggtggggaa gcgaccagcg gattctttga agtgccgaaa aacgaaacca aagaaaatgg
    21241 aattcgtctt tccgagcgta aagagacact gggtgatgtg acgcaccgaa ttctgacagt
    21301 gccgattgcg caggaccagg tggggatgta ttaccagcag ccagggcaac aactggcaac
    21361 ctggattgtt ccgccgggac aatacttcat gatgggcgac aaccgcgaca acagcgcgga
    21421 cagccgttac tggggctttg tgcctgaagc gaatctggtc ggtcgggcca cggctatctg
    21481 gatgagcttc gataagcaag aaggcgaatg gccgactggt gtgcgcttaa gtcgcattgg
    21541 cggcatccat taatagccat cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt
    21601 atttatgaga taaatctccc gtggctaacg acatcccccg tcgttgtgta tagaatattc
    21661 ccccgaagtt taaggttggc accttcaggt tgccacggca cacgaaacag cgttggtccc
    21721 catataccgc taaactgaaa ctgcagcgaa gcagttagca gagccatgta tatcaggtct
    21781 gtttcgtgtg ctgaattgtt gacgcattta tttattggta tcgcatgaac cccatcgtaa
    21841 ttaatcggct tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg
    21901 cattaactca tcgtagtgcc agcagcaaac ataacgagcg tttagaattt ttaggcgact
    21961 ctattctgag ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag
    22021 gcgatatgag ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc
    22081 gcgaatttga gttaggcgag tgcttacgtt tagggccagg tgaacttaaa agcggtggat
    22141 ttcgtcgtga gtcaattctc gccgacaccg tcgaagcatt aattggtggc gtattcctcg
    22201 acagtgatat tcaaaccgtc gagaaattaa tccttaactg gtatcaaact cgtctggacg
    22261 aaattagccc aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg
    22321 gtcgccatct gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc
    22381 aggaatttac tatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt
    22441 caagccgtcg taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg
    22501 aatgagcatc gataaaagtt actgcggatt tattgccatc gtcggacgtc cgaacgttgg
    22561 caaatccaca ttgttgaaca aactgctggg gcagaaaatc tccatcacct cccgcaaggc
    22621 gcagacaact cgtcaccgca ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta
    22681 cgtcgataca ccgggcctgc atatggaaga aaaacgcgcc attaaccgcc tgatgaacaa
    22741 agcggcgagc agctctattg gcgatgttga gctggtgatt tttgtcgttg aaggcacccg
    22801 ctggacgccg gacgacgaaa tggtgctcaa caaactgcgc gaaggcaaag cgccggtaat
    22861 cctcgcggtg aacaaagtgg acaacgtgca ggagaaagcc gatctgctgc cgcacctgca
    22921 gttcctggca agccagatga acttcctcga tatcgtgcca atctctgccg aaaccgggct
    22981 gaatgttgac actattgcgg caatcgtgcg taagcatctg cctgaagcga ctcatcactt
    23041 cccggaagat tacatcaccg atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga
    23101 aaaactgatg cgtttccttg gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg
    23161 tttcgtctct aacgaacgcg gcggttgtga catcaacggt ttgattctcg ttgagcgtga
    23221 agggcagaag aagatggtta ttggcaacaa aggggccaag atcaaaacca tcgggattga
    23281 agcgcgtaaa gacatgcagg aaatgttcga agcgcctgtc caccttgaac tgtgggtaaa
    23341 agtgaaatcg ggttgggccg acgacgaacg cgcactgcgc agtctcggtt atgttgacga
    23401 tctttaagag tcactccgat ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg
    23461 tggagcgaaa ccagcctgat gctggacgtc ttcacagagg aatcgggtcg cgtgcgtctg
    23521 gttgccaaag gcgcacgctc taaacgctct actctgaaag gtgcattaca gcctttcacc
    23581 cctctcttgc tacgttttgg cgggcgtggc gaagtcaaaa cgctgcgcag tgctgaagct
    23641 gtctcactgg cgctgccatt aagcggtatc acgctttaca gcggtctgta catcaacgaa
    23701 cttctctccc gcgtactgga atacgagacg cgcttctctg aactcttttt cgattacttg
    23761 cactgcattc agtctcttgc aggtgacact ggtacgccag aacccgcgct gcgtcgcttt
    23821 gaactggcac tactcgggca tctgggctat ggcgtcaatt ttacccattg cgcgggtagc
    23881 ggcgagccgg tagatggcac catgacgtat cgttatcgcg aagaaaaagg gtttatcgca
    23941 agcgtcgtta tcgacaataa aacgttcacc ggaaggcagt taaaagcgtt aaacgcgcgg
    24001 gaatttcctg acgcagacac actacgcgcc gcgaaacgct ttacccgaat ggcgcttaag
    24061 ccgtatcttg gcggtaaacc attaaagagc agggaactgt tccggcagtt tatgcctaag
    24121 cgaacggtga aaacacatta tgaatgatga ggattgtcat ggctgaatta ctgttaggcg
    24181 tcaacattga ccatattgca acgctgcgta acgcacgcgg tactgcttac ccggatccgg
    24241 tgcaggcggc gtttattgcc gagcaggcgg gagcggacgg cattaccgtg catttacgtg
    24301 aagatcgccg ccacattact gaccgcgatg tgcgcatcct gcggcagacg ctggataccc
    24361 gaatgaatct ggagatggca gtgaccgaag agatgctggc gatcgccgtt gagacgaagc
    24421 cacatttttg ctgcttggta ccggaaaagc gtcaggaagt aacaaccgaa ggcggcctgg
    24481 acgttgcagg gcagcgtgaa aaaatacgcg atgcctgcaa acgtctggca gatgccggga
    24541 ttcaggtttc tctgtttatc gacgccgatg aagagcagat caaagctgcg gcagaggttg
    24601 gcgcaccgtt tatcgagatc cacaccggtt gctatgctga tgccaaaact gacgccgaac
    24661 aggcgcaaga gctggtgcgt atcgccaaag ccgcgacctt tgccgctagt ctcggtctga
    24721 aagttaacgc cggacacggt ctgacctatc acaacgtgaa agccattgcc gccatccctg
    24781 agatgcatga actgaatatc ggtcatgcca ttattggccg tgcagtgatg accggactga
    24841 aagatgcggt ggcagaaatg aagcgtctga tgctggaagc gcgtggctaa tggcaatatt
    24901 aggtttaggc acggatatcg tagagatcgc tcgcatagaa gcagtgatcg cccgatccgg
    24961 tgagcgcctg gcacgccgcg tattaagcga taacgaatgg gcaatctgga aaacgcacca
    25021 ccagccggtg cgttttctgg cgaagcgttt tgctgtgaaa gaagccgcag caaaagcgtt
    25081 tggcaccggg atccgcaatg gtctggcgtt taatcaattt gaagtattca atgatgagct
    25141 cggcaaacca cggctacggc tatggggtga ggcattaaaa ctggcggaaa agctcggcgt
    25201 tgtaaatatg catgtaacgc tggcagatga acgccactat gcttgtgcca cggtaattat
    25261 tgaaagttaa aataataccg ttctctttaa aaagtaaaac ccccgaactg gtaaggaacg
    25321 ggggttttac tttttaactt taattttgtg cagcatgcac cctcaacaac agcgattatg
    25381 gatcagtgta cttatctttt acaagcaact tgcaatcttt agcataaaaa ctcgagcctt
    25441 tacgaagaaa gcaatattga tggaaagatt aacgtgaccg ccaattcgta agtacattaa
    25501 aattggcttc gttattgaag attttgctgt gctttacacc atgccacaga attcccccat
    25561 tgaaacgagt ggtgtcgtca aagctctggt gtggagtgca gcatgcaccc tcaataactc
    25621 gcacgttcag ttttggggag atgtaagggc taatctgaat ggctgcattc cttgtttaag
    25681 gaaaaacgaa tgactgattg ccgatacctg attaaacggg tcatcaaaat catcattgct
    25741 gttttacagc tgatccttct gttcttataa cacaaggaaa cgtacttaag gtgcgtccgg
    25801 tgaaccaatc ggacgcgcct ttaataacta taaataagtg tcttggcaga tactatataa
    25861 attaacttag tgaatgatta tgctaatgtc atcaattaaa taaatataat ggcgttaagg
    25921 cttcccagta atataattaa tactctactt ccagagtaga atattaaatt ttatccgcgt
    25981 ggtgcatcag cacaaattta tcccacaact gttcttctgt ctcgacatgc gccggatctt
    26041 tcacaatagt attggggatc gggcacacct tctggcaggt tggtgtctcg tagtgcccta
    26101 cgcattcggt acacttatcg ctgttaattt cgtagatatg atctcccatt gaaatcgcct
    26161 cattcgggca ttcgggttca cacatatcac aattaataca gcgtttagta attagtaaag
    26221 acatttcaat ggattaccgt taaatcattt taaaatcagt aagttgtatc gagtttgtat
    26281 gctttactgt cattaactta ctgtatgttg atccagtgta tttaaccttg ataaactcag
    26341 tccagcaaca caaaaccgca acacattgca ttttgtcccg tagaaaagac ttgtatgtgt
    26401 gagcttgttt tctgcgccta cgcagataag gattgagaat gccgcgcact gtaacacata
    26461 atccggatag ccccaataat gacgatgttt tagccgcatc tgaaaaatgg gacgcctgta
    26521 aaccccccta taccagcgca cacatgaaaa tctgtgttgc tgccgccaaa atcatcctcg
    26581 ctgcttccgg cgtggctcgc tgttccaaat acgaaaaaga gaactatctc cgtatcgatt
    26641 tcagcaaagc cggtaaggtt acattttacg ccgagtttcc aaaaaagatg ggcctcaaag
    26701 gtaaaaagct cggcgagtgg ccggagctcg ttatccagct ggcgcgcgaa aaagcactag
    26761 gtatggctga cggtggactg cgggcagagt ccgtacatgc agcgctggaa atgtaccggg
    26821 atgacccaaa gccaaagtag cccggcagaa gctgagcccg gacagtttca caacctacgg
    26881 ggtgcgtatc gaccggatta aagtaacgtt cggcgagcgt gaggtgttca gcgacgtaac
    26941 atacaatcgg ctggtggaag tgctggacga gtggatcgcc actcgctcga acaataacgc
    27001 cctggagttg tttgccgagc tccgtcggtt ctggaagttc tgcgcaccta ctctttgcaa
    27061 cggccgcaat gttgccgcca gtctgccaga tgattatgtt tcctcccgcg tacagaaacc
    27121 tacccccaca cggcttttta ccgatattga atcaatcgcc cgactctggc tcaatgttgc
    27181 tgcctgcacc tctgtacacc agaagaatgc tgttcgcttc atgatcatca ctggtgttcg
    27241 tccgattaat gtccataacc tgcgctggga ctacgtttac gaggaggctg gtgaaattgt
    27301 ttatccggaa ggggttatcg gcatgcgagg ggctatgaaa acacaaaagg ctttccgcct
    27361 gccgataacg cctgagatcc ggcggattat cgacgagcag aaagcctggc gtgattcagt
    27421 tcctgagtgc aacagggatt atgtattttt gcagccacgt gatccaatgc agccattttc
    27481 aaaacgatca ctggataagc tggtgaaaac atacagcccg gacggggctg taaaatgagg
    27541 tgtactggca atagcggaca ctaccatttg ttcttttttt aagcagccat ctgatgat
//
LOCUS       DADLEE010000056        26564 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000056 DADLEE010000000
VERSION     DADLEE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26564
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.056"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            348..641
                     /locus_tag="JQH51_002445"
     CDS             348..641
                     /locus_tag="JQH51_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076755.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="JQH51_002446"
     CDS             713..1372
                     /locus_tag="JQH51_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY6076756.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1449..1910
                     /locus_tag="JQH51_002447"
     CDS             1449..1910
                     /locus_tag="JQH51_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY6076757.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="JQH51_002448"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="JQH51_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /gene="umuD"
                     /locus_tag="JQH51_002449"
     CDS             3390..3809
                     /gene="umuD"
                     /locus_tag="JQH51_002449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY6076758.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /locus_tag="JQH51_002450"
     CDS             3809..5077
                     /locus_tag="JQH51_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY6076759.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="JQH51_002451"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="JQH51_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY6076760.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="JQH51_002452"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="JQH51_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY6076761.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="JQH51_002453"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="JQH51_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY6076762.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="JQH51_002454"
     CDS             complement(8332..9864)
                     /locus_tag="JQH51_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY6076763.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /locus_tag="JQH51_002455"
     CDS             10194..11492
                     /locus_tag="JQH51_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY6076764.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="JQH51_002456"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="JQH51_002456"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY6076765.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="JQH51_002457"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="JQH51_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="JQH51_002458"
     CDS             complement(14765..16501)
                     /locus_tag="JQH51_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY6076766.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="JQH51_002459"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="JQH51_002459"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY6076767.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="JQH51_002460"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="JQH51_002460"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY6076768.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="JQH51_002461"
     CDS             complement(18223..18957)
                     /locus_tag="JQH51_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY6076769.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="JQH51_002462"
     CDS             19158..19412
                     /locus_tag="JQH51_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY6076770.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="JQH51_002463"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="JQH51_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21442..22296)
                     /locus_tag="JQH51_002464"
     CDS             complement(21442..22296)
                     /locus_tag="JQH51_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY6076771.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="JQH51_002465"
     CDS             complement(22296..23105)
                     /locus_tag="JQH51_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY6076772.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="JQH51_002466"
     CDS             complement(23115..23873)
                     /locus_tag="JQH51_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6076773.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="JQH51_002467"
     CDS             complement(23870..24850)
                     /locus_tag="JQH51_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY6076774.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="JQH51_002468"
     CDS             complement(24850..25872)
                     /locus_tag="JQH51_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY6076775.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26222..>26564
                     /locus_tag="JQH51_002469"
     CDS             26222..>26564
                     /locus_tag="JQH51_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076776.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6674 a         6472 c         6855 g         6563 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat tatttaaata tgaatgacat tattgttttt ttatcgtcca
    26101 cggttacgcg acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag
    26161 aaaattatca gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag
    26221 aatgctgggg gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag
    26281 taagcagcgt aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat
    26341 taatgtcatt aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc
    26401 gtttattcgg acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct
    26461 ttattttgat aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga
    26521 gttgcagtta ttaagcacag ggtgtagcaa tgataacatt ctcc
//
LOCUS       DADLEE010000057        25999 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000057 DADLEE010000000
VERSION     DADLEE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25999
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.057"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            69..176
                     /locus_tag="JQH51_002470"
     CDS             69..176
                     /locus_tag="JQH51_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY6076777.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(263..1942)
                     /gene="bcsG"
                     /locus_tag="JQH51_002471"
     CDS             complement(263..1942)
                     /gene="bcsG"
                     /locus_tag="JQH51_002471"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY6076778.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1939..2130)
                     /gene="bcsF"
                     /locus_tag="JQH51_002472"
     CDS             complement(1939..2130)
                     /gene="bcsF"
                     /locus_tag="JQH51_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY6076779.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2127..3698)
                     /gene="bcsE"
                     /locus_tag="JQH51_002473"
     CDS             complement(2127..3698)
                     /gene="bcsE"
                     /locus_tag="JQH51_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY6076780.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3971..4159
                     /locus_tag="JQH51_002474"
     CDS             3971..4159
                     /locus_tag="JQH51_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076781.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4171..4923
                     /gene="bcsQ"
                     /locus_tag="JQH51_002475"
     CDS             4171..4923
                     /gene="bcsQ"
                     /locus_tag="JQH51_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY6076782.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4920..5279
                     /locus_tag="JQH51_002476"
     CDS             4920..5279
                     /locus_tag="JQH51_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY6076783.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5276..7537
                     /gene="bcsA"
                     /locus_tag="JQH51_002477"
                     /pseudo
     CDS             5276..7537
                     /gene="bcsA"
                     /locus_tag="JQH51_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7548..9887
                     /gene="bcsB"
                     /locus_tag="JQH51_002478"
     CDS             7548..9887
                     /gene="bcsB"
                     /locus_tag="JQH51_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY6076784.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9894..11000
                     /gene="bcsZ"
                     /locus_tag="JQH51_002479"
     CDS             9894..11000
                     /gene="bcsZ"
                     /locus_tag="JQH51_002479"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY6076785.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11077..11418
                     /locus_tag="JQH51_002480"
     CDS             11077..11418
                     /locus_tag="JQH51_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY6076786.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11333..11584
                     /locus_tag="JQH51_002481"
                     /pseudo
     CDS             11333..11584
                     /locus_tag="JQH51_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11666..13654
                     /gene="hmsP"
                     /locus_tag="JQH51_002482"
     CDS             11666..13654
                     /gene="hmsP"
                     /locus_tag="JQH51_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY6076787.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13845..15131
                     /locus_tag="JQH51_002483"
     CDS             13845..15131
                     /locus_tag="JQH51_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY6076788.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15352..16848
                     /locus_tag="JQH51_002484"
     CDS             15352..16848
                     /locus_tag="JQH51_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY6076789.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16944..18092)
                     /locus_tag="JQH51_002485"
     CDS             complement(16944..18092)
                     /locus_tag="JQH51_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY6076790.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18105..18872
                     /gene="pdeH"
                     /locus_tag="JQH51_002486"
     CDS             18105..18872
                     /gene="pdeH"
                     /locus_tag="JQH51_002486"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY6076791.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18942..21002
                     /locus_tag="JQH51_002487"
     CDS             18942..21002
                     /locus_tag="JQH51_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY6076792.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21184..22506)
                     /locus_tag="JQH51_002488"
     CDS             complement(21184..22506)
                     /locus_tag="JQH51_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY6076793.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22917..23930)
                     /gene="yhjD"
                     /locus_tag="JQH51_002489"
     CDS             complement(22917..23930)
                     /gene="yhjD"
                     /locus_tag="JQH51_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY6076794.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23979..24950)
                     /locus_tag="JQH51_002490"
     CDS             complement(23979..24950)
                     /locus_tag="JQH51_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6076795.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            25398..>25999
                     /locus_tag="JQH51_002491"
     CDS             25398..>25999
                     /locus_tag="JQH51_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY6076796.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6196 a         6532 c         7072 g         6199 t
ORIGIN      
        1 ggccattctt gaacctgaac aactcaagaa tagccgcgaa acgttgtcat tacaacacag
       61 gcggctatat gacgctcgca gaactgggca tggccttctg gcatgattta gcggctccgg
      121 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaataagcgg aagtaacgtg
      181 tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc ggatcaggcc gcagaggtta
      241 aactctgcgg cctttttcgt gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc
      301 gaacgtacgg tttatcttgg tattgaataa ctactgcatt tgagttctcg gagaccggtg
      361 ctgtttgtgg caacccactg gtgagttttt tccagtcaac attgtcttcg gtgaaaatct
      421 tgccatcaag aacgcgaacc accagatcgg agatagccag gaagctgctc ggttggtcaa
      481 tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga
      541 cgtctgtgat agacgggcta gggatatcac gtaggccaga tacctgcatt ctgtcgccct
      601 tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac tttacgaccc gatttctcca
      661 gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg cgcccgcgct ttgtaatccg
      721 ctgttttgct gacccccgga taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg
      781 tggcactacg gctgttttta tctttttcgg taacgtccag ccagcggtta agcacggcgg
      841 tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat
      901 ccatcaattc agtctgcatg ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact
      961 ggccgttatg tcccatcatc aggtgctggg taaagcccag tttcgaaagg ttatcaaaca
     1021 gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc ccgcagctgg
     1081 cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta aagttcttga
     1141 aatcaatatc gaaatgcgac cacagtggat gcgacatcaa cccggcggct tctatatccg
     1201 accaggaaag cgaacagatg ttaatcacca gtagttcaaa tggctgagca tcagcgggca
     1261 gcgaagacgg gaaggtcgat ttacgtttcg cctccgcgtt atagaaatta ttcagccagg
     1321 cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg catatcaccc actaccggtg
     1381 cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg
     1441 tcggttgtcc ggctggccac aaggagaaac ttggtcccgc cagggtaagt acgttcagcc
     1501 atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg
     1561 ccactaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt gtgacaaggt
     1621 cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt atgctttccg
     1681 ggccaggcaa ccagatgtca tgccagaaca aagcaaagcc gatcggcagg gcaatccagt
     1741 ggcgcaagcg atgcaggctg taacgcggaa tgggcatcag cagaaacgcg gcaaacacca
     1801 aattgaggag cgggtggaag ttaagatatc ccgcccacaa caggccaaac ttaaccagaa
     1861 aatagaagtt ccagccggaa aggccgcgcc agtattgcca gagggaagaa ggcatggcgg
     1921 tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc ggcgtaacgt
     1981 cccggccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg
     2041 tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc aaacgataat
     2101 aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc atctaacagt
     2161 cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt
     2221 ttagaacttt gcgttaaagg cagcggcatt ccccattgtt ctggggcaag caggcgcatc
     2281 tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg
     2341 tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt aatccgacag
     2401 aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc ggtacggtta
     2461 gggcgacata gcgtcagggc ttgctcaacg cggatacccg gtaccggacg tagggcaacc
     2521 agaacgcctt taccgtgggc aggcaagaga gggttattca tcatgttgtt gacggcatta
     2581 cagaacacat cccacttctg gaaaccacgc agtttgagcg gctgggtcat tgacagcaag
     2641 gtagtgatat cttccggtac atagcgacta aacttctgcc cttgcacgct ttcgatcatc
     2701 gtcagacaac gggagagtgg cgcattccac ggaataacca tatttgcacc gcaggccaat
     2761 aacaaacgtt catcggtggc gcgcaggcta gcggtatttt cccgcacgag gattttcatc
     2821 gcactaccgc gctggcgacg cagggtatga atgctgcggg ccagtggctc gatttgcgca
     2881 ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc attgaacagg
     2941 acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca
     3001 gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt
     3061 tgaactaatg tccagatacc attttgttgc tgaacgctaa gctgctgacg ggcgctgacc
     3121 cctttttcgt tgcaccagaa ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa
     3181 ctggcaagac caaaaagtga acggtattcc tcaagcaaca atgaaaattg tttatcgtta
     3241 ttatttccgg gattaattac caaaagcgaa caatggttta acctgctcca tttattcatt
     3301 ttatccaacc atgagcgaag ccgctcggca ggaatatttt gccatgcgtt atttgcgcaa
     3361 acaagaataa aaaggtaatt atggggatca atagaacact gcaaatcacg ggccaaatag
     3421 tatagacctt tttcatgatt tagcattgaa aataatttta ttttttccgg cccttgagaa
     3481 tcatctaatt gaaagatttt cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg
     3541 gtttcagcct gggatgcaat tgtttgattc gccagactga tagcatcttc atggcgatcg
     3601 acgttaaacc accagacgcc gcctgctggc atatgtcgca gctcatccca taatgatgag
     3661 ataccgatag agaatacagg gtccacaatg tccctcatgt caccattttg tctgattatc
     3721 agtttactag cgaaagctaa gaaataaacc taccattgaa attaaaactt atcagattta
     3781 gcatgtgaat caatttagaa ggtcacaaga cgtgtgattt tagtcttttc gtttttaact
     3841 ttttctgttg cgtaacggaa agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat
     3901 aaaccggcga aatgctcacg gttagaattg tctcatgaat ggtacggtta tttcataggg
     3961 atcaagcaaa atgaataaca atgaaccaga tactctgcct gatcccgcga taggctatat
     4021 cttccagaat gatattgtgg cgttaaagca ggcattttca ctgcctgata ttgattatgc
     4081 cgatatttcc caacgcgaac agttggccgc ggcattaaaa cgctggccgt tgctggcaga
     4141 gtttgcgcaa caaaagtagg ggattggtga atggccgtac tgggattgca gggggtgcgg
     4201 ggaggcgtgg ggacaacaac catcaccgcc gcattagcct ggtcattaca aatgttggga
     4261 gaaaatgtcc tggtggtcga tgcctgcccg gacaacttgt tgcgcctgtc atttaatgtt
     4321 gattttaccc accgtcaggg ctgggccaga gcgatgctgg atggccagga ctggcgtgac
     4381 gctgggttgc gctacacctc gcagctcgat ttgctgcctt ttggtcagtt atccattgaa
     4441 gaacaagaaa atccacagca ctggcaaacc cggctgagcg atatttgctc cggcttacag
     4501 caactaaaag ccagcgggcg ttaccagtgg attttaatcg acttaccgcg tgatgcctcg
     4561 cagataaccc accagctgct gagtttgtgc gagcactcgc tggcaatcgt caatgtggat
     4621 gccaactgcc atatccgact gcatcagcaa gcgctgccgg atggcgcaca tattttgatt
     4681 aatgacttcc gtattggcag tcaggttcag gacgatattt accagctttg gttgcaaagc
     4741 cagcgccgat tactgccgat gctcattcat cgtgatgaag cgatggctga atgcctggcg
     4801 gctaagcaac cagtaggtga atatcgcagt gatgcgctgg cggctgaaga gatactgacg
     4861 ctggcgaact ggtgcctgtt gaactactcc gggctgaaaa cgccagtcgg gagtgcatca
     4921 tgagtatcct gacccggtgg ttgcttatcc cgccggtcaa cgcgcggctt atcgggcgtt
     4981 atcgcgatta tcgtcgtcac ggtgcgtcgg ctttcagcgc gacgctcggc tgtttctgga
     5041 tgatcctggc ctggattttt attccgctgg agcacccgcg ctggcagcgt attcgcgcag
     5101 aacataaaaa cctgtatacg catatcaacg cctcgcgtcc gcgtccgctg gacccggtcc
     5161 gttatctcat tcaaacatgc tggttattga tcggtgcatc gcgcaaagaa acgccgaaac
     5221 cgcgcagcgg gcattttcag gtctgcaaaa tattcgtgga cgttaccatc aatggatgaa
     5281 cgagctgcct gagcgcgtta gccataaaac acagcatctg gatgaaaaaa aagagctcgg
     5341 tcatttgagt gccggggcgc ggcggttgat cctcggtatc atcgtcacct tctcgctgat
     5401 tctggcgtta atctgcgtta ctcagccgtt taacccgctg gcgcagttta tcttcctgat
     5461 gctgctgtgg ggggtagcgc tgatcgtacg gcggatgccg gggcgcttct cggcgctaat
     5521 gttgattgtg ctgtcgctga ccgtttcttg ccgttatatc tggtggcgtt acacctctac
     5581 gctgaactgg gacgatccgg tcagcctggt gtgcgggctt attctgctct tcgctgaaac
     5641 gtacgcgtgg attgtgctgg tgctcggcta cttccaggta gtatggccgc tgaatcgtca
     5701 gccggtgcca ttgccgaagg atatgtcgct gtggccgtca gtggatatct ttgtcccgac
     5761 ttacaacgaa gatctcaatg tggtgaaaaa taccatttac gcctcgctgg gtatcgactg
     5821 gccgaaagac aaactgaaca tctggatcct cgatgacggc ggcagggaag agtttcgcca
     5881 gtttgcgcaa aacgtggggg tgaaatatat cgcccgcacc actcatgaac atgcgaaagc
     5941 gggcaacatc aacaatgtgc tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg
     6001 cgaccacgta ccaacgcgat cgttcctgca aatgaccatg ggctggttcc tgaaagagaa
     6061 acagctggcg atgatgcaga ctccacacca cttcttctcg ccggacccgt ttgaacgcaa
     6121 cctgggacgt tttcgtaaaa cgccgaacga aggcacgctg ttctatggtc tggtgcagga
     6181 tggcaacgat atgtgggacg ccactttctt ctgcggttcc tgtgcggtga ttcgtcgtaa
     6241 gccgctggat gaaattggcg gcattgctgt cgaaaccgtg actgaagatg cgcatacttc
     6301 tctgcgtttg caccgtcgtg gctatacctc cgcgtatatg cgtattccgc aggcggcggg
     6361 gctggcgacc gaaagtctgt cggcgcatat cggtcagcgt attcgctggg cgcgcgggat
     6421 ggtataaatc ttccgtctcg ataacccgct caccggtaaa ggactgaagt ttgctcagcg
     6481 gctgtgctac gtcaacgcca tgttccactt cttgtcgggc attccacggc tgatcttcct
     6541 gactgcgccg ctggcgttcc tgctgcttca tgcctacatc atctatgcgc cagcgttgat
     6601 gatcgcccta ttcgtgctgc cgcatatgat ccatgccagc ctgaccaact cgaagatcca
     6661 gggcaaatat cgccactctt tctggagtga aatctacgaa acggtgctgg cgtggtatat
     6721 cgcaccaccg acgctggtgg cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc
     6781 caaaggtgga ctggtggaag aagagtacgt cgactgggtg atctcgcggc cctacatctt
     6841 ccttgttcta ctcaacctgg tgggcgttgc ggtaggcatc tggcgctact tctatggccc
     6901 gccaaccgag atgctcaccg tggtcgtcag tatggtgtgg gtgttctaca acctgattgt
     6961 tcttggcggc gcagttgcgg tatcggtaga aagcaaacag gtacgccgat cgaaccgcgt
     7021 ggagatgacg atgcccgcgg caattgcccg cgaagatggt cacctcttct cgtgtaccgt
     7081 tcaggatttc tccgacggtg gtttggggat caagatcaac ggtcaggcgc agattctgga
     7141 agggcagaaa gtgaatctgt tgcttaaacg cggtcagcag gaatacgtct tcccgaccca
     7201 ggtggcgcgc gtgatgggta atgaagttgg gctgaaatta atgccgctca ccacccagca
     7261 acatatcgat tttgtgcagt gtacgtttgc ccgtgcggat acatgggcgc tctggcagga
     7321 cagctacccg gaagataagc cgctggaaag tctgctggat attctgaagc tcggcttccg
     7381 tggctaccgc catctggcgg agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct
     7441 gacttctctg gtttcctggg ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac
     7501 ggcacaacca tcggatcagg ctttggctca acaatgatga taacgcgatg aaaagaaaac
     7561 tattctggat ttgtgcagtg gctatgggga tgagtgcgtt cccctctttc atgacgcagg
     7621 cgacgccagc aacgcaacca ctgatcaatg ctgagccagc tgtagccgcc cagacggaac
     7681 aaaatccgca ggtggggcaa gtgatgtcag gcgagcaggg agctgatgcc cccatcgtgg
     7741 cgcagaacgg tccttcgcgt gatgtgaagc tgacctttgc gcaaatcgcg ccgccgccgg
     7801 gcagcatggt gctacgtggt attaacccga acggcagcat tgagtttggt atgcgcagcg
     7861 atgaagtggt gacgaaggcg atgctcaact tcgaatacac gccatcgcca tcgttactgc
     7921 ctgtccagtc gcagttaaag gtttatctca atgatgagct gatgggcgtg ctgccagtga
     7981 ccaaagaaca gttgggtaaa aaaacgctgg cgcaaatgcc cattaatcca ctgtttatta
     8041 ccgacttcaa ccgtgtgcgg ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc
     8101 cggccagcac cacgctttgg ctggatgttg ggcggagcag tggactggat ctgacctatc
     8161 agaccctgaa tgtgaagaat gacctgtcac acttcccggt gccattcttt gacccgcgcg
     8221 ataaccgtac taacaccttg ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac
     8281 aagcatctgc cattgtcgcc tcgtggtttg gttcgcgttc tggctggcgt gggcagaact
     8341 tcccggtgct ctataaccaa ttgccggatc gcaatgccat tgtctttgca accaacgaca
     8401 aacggccgga cttcctgcgc gatcatccgg cggtaaaagc cccggtgatt gagatgatta
     8461 accatccgca gaatccttac gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc
     8521 tgttgcaggc agcgaaaggt atcgctcagg gtaacattct gttccgtggt gaaagcgtgg
     8581 tagtgaatga agtgaaaccg ctgctaccgc gtaagccgta cgatgcgccg aactgggtac
     8641 gtaccgatcg tccggtcaca tttggcgaac tgaaaaccta tgaagaacag ttacaatcca
     8701 gcggccttga gccagcagcg attaacgttt cgctaaacct gccgccggat ctctacctga
     8761 tgcgcagtac cggcattgat atggatatta attaccgcta caccatgccg ccggtgaaag
     8821 acagttcgcg gatggatatc agcctgaata accagttcct gcaatccttc aacctgagca
     8881 gcaaacagga ggcgaaccgt ctgctgctgc gtattccggt attacaaggt ttgctggatg
     8941 gcaaaacaga tgtctctatt ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg
     9001 actttgagta tatgaacccg atgccaggcg gttcggtaga taactgtatt accttccagc
     9061 cggtgcagaa tcatgtggtg attggtgacg attccaccat cgacttctcg aagtattacc
     9121 acttcatccc gatgccggat ctacgcgcct ttgctaacgc gggcttccca ttcagccgga
     9181 tggcggatct gtcgcaaacc atcaccgtga tgccgaaagc gcctaacgaa gcacagatgg
     9241 aaacgttgct gaatactgtt ggttttatcg gcgcacagac gggcttcccg gcgattaatc
     9301 tgacggtgac cgatgatggc agcaccattc agggcaaaga tgccgacatc atgatcatcg
     9361 gtggtatccc ggacaaactg aaagacgata agcagatcga cctattggtg caggcgaccg
     9421 aaagctgggt gaaaacaccg atgcgccaga ccccgttccc cggcattgtg ccggacgaga
     9481 gcgatcgcgc ggcagaaacc cagtcaacgc tgacctcttc cggtgcgatg gcggcggtga
     9541 ttggcttcca gtcgccgtat aacgaccagc gcagcgtgat tgcgctgttg gcagatagcc
     9601 cacgcggtta tgaaatgctt aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt
     9661 tcggttcggt cgcggtgatc cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt
     9721 attacgtagg tcatctgccg tggttcgagc gcttgtggta tgcgctggca aaccatccga
     9781 ttctgctggc ggtgctggcg actatcagtg tgatattgct ggcatgggta ctgtggcgtc
     9841 tgctgcgaat tattagtcgt cgtcgtctta acccggataa cgagtaattg aagatgaatg
     9901 tgttgcgtag tggaatcgtg acgatgctgt tgttggctgc ctttagtgtt caggcagcct
     9961 gtacctggcc tgcctgggag cagtttaaaa aggattacat cagtcaggaa gggcgcgtca
    10021 tcgaccccag cgacgcgcgc aaaatcacca cctccgaagg gcaaagttac ggtatgttct
    10081 ttgccctggc ggctaacgac cgtgcagctt tcgataatat tctcgactgg acgcagaaca
    10141 atctcgctca gggttcttta aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga
    10201 acagtaagtg ggaagtgctg gacagcaatt cggcctccga tggtgatgtc tggatggcct
    10261 ggtcgttgct ggaggcgggg cgtttgtgga aagagcagcg ttataccgac atcggcagcg
    10321 cgttgctaaa acgtatcgcg cgggaggaag tggtgacggt gcctgggctg ggttccatgt
    10381 tgttaccggg caaagtgggt tttgctgagg ataacagctg gcgttttaac cccagctaca
    10441 tgccgccgac gctggcgcag tatttcaccc gctttggcgc gccgtggacc acgctgcgcg
    10501 aaaccaatca acatttattg ctggaaaccg ccccgaaagg cttttcgcca gactgggtgc
    10561 gctatgagaa agacaaaggc tggcagctaa aagccgaaaa aacattgatc agcagctacg
    10621 acgctatccg cgtttacatg tgggtaggca tgatgcctga cagcgatccg caaaaagcgc
    10681 ggatgctcaa ccggtttaaa ccgatggcga cattcactga gaaaaacggt tatccgccgg
    10741 aaaaagtgga tgtggctacg gggaaagcgc agggtaaagg accggtcggt ttttctgccg
    10801 ccatgctgcc ttttttacaa aaccgcgatg cgcaggcagt tcagcgccag cgcgtggccg
    10861 ataactttcc cggcagcgat acctattaca actatgtgct gaccctgttt ggacaaggct
    10921 gggatcaaca ccgtttccgc ttctcgacaa aaggtgagtt attacctgac tggggccagg
    10981 aatgcgcaaa ttcacactaa acatattcac gctttccctc ggtctggccg tcatgccgat
    11041 ggtcgaggca gcaccaacca tcagcagacc ctgcggatgg acagcggcaa cactaatgcc
    11101 gtgcgcgggc tggcgaatat ttaccgccag cagtcgccag aaaaagctga agcgtttatc
    11161 gcctcgctct ctgccagcca gcggcgcagc attgatgata tcgaacgcag cctgcaaaac
    11221 gaccgtctgg cacagcaggc agaggtactg gaaaaccagg gcaaatgggc gcaggcggca
    11281 gcacttcagc ggcaacgact ggcgctgggc cccggcagcg tatggattac ttaccgactg
    11341 gcaggaagaa gctgcgcggc aatccaacga tggcggcagc agtcagggct tcggctacac
    11401 ggcgcgggca ttacttgaac gacgtgttac ttccaactgg tttgttggca cggcaattga
    11461 tatccagcag gtgaaagatt acgcacccag ccatttcctg ctctacgtac gttattccgc
    11521 cgccggatgg cagggtgaca tggatttacc gccgcagccg ctgatacctt acgccgactg
    11581 gtaagttttc agatagcgcc tctcttaatg ccgctgcgat cgggtatact cgggcggcaa
    11641 tctgggattt ccggggggag acaatttgcg cgtaagtcgc tcgttaacaa tcaagcagat
    11701 ggcaatggtg gcagccgttg tcctggtgtt cgtttttatt ttttgcaccg ttttgctgtt
    11761 ccatctggtc cagcagaatc gctataacac ggctacgcaa ctggaaagca ttgctcgctc
    11821 tgtccgcgaa cccttatctt cagctatttt gaaaggcgat attcccgaag cggaagctat
    11881 tcttgccagc attaaaccgg caggcgtggt cagccgtgcc gatgtagtgc tgcctaacca
    11941 gttccaggcg ctgcgtaaaa gttttattcc agagcgtccg gtgccggtaa tggttactcg
    12001 cctgtttgag ctaccggttc aaatctcgct gggcgtctac tcgctcgaac gtccggcaaa
    12061 tccgcagcca atagcctatc tggtgctaca ggcggattcc ttccgtatgt ataagttcgt
    12121 gatgagcact ctctcaacgt tagtgaccat ttacttactt ttgtcgctta tcctgaccgt
    12181 cgccatcagc tggtgcatta accgcctgat tttgcatccg ttacgcaata ttgctcgcga
    12241 acttaacgcc atcccagccc aggagcttgt tggtcaccaa ctggcattac cgcgtctgca
    12301 tcaggacgat gaaatcggta tgttggtgcg cagttacaac ctcaaccagc aattgctgca
    12361 gcgccattat gaagaacaga acgaaaatgc gatgcgcttc ccggtgtcgg atttgccgaa
    12421 caaagccttg ctgatggaga tgctggagca ggttgtcgcg cgtaaacaaa ccaccgcgct
    12481 gatgatcatc acctgtgaaa ccctgcgtga tactgcgggc gtgctgaaag aggcgcaacg
    12541 agaaattctg ctgctgacgc tggtggaaaa actcaaatcg gtactgtcgc cacgtatgat
    12601 cctcgcgcag attagcggtt atgactttgc tgtcattgcc aacggtgtac aggaaccgtg
    12661 gcacgcaatc actttgggtc agcaagtact cactatcatg agcgagcgcc tgccgattga
    12721 acgtattcaa ctccgtccgc actgtagcat tggcgtggcg atgttctacg gcgatctcac
    12781 cgccgaacag ctttacagtc gcgctatttc tgcggcattt accgctcgcc ataaaggcaa
    12841 gaatcagatt cagttctttg atccgcagca gatggaagca gcccagaagc ggttgacgga
    12901 agagagcgat atccttaatg cactggaaaa tcatcagttt gcaatttggt tacagccaca
    12961 ggtcgagatg accagcggta aactggtcag tgcggaagtg ttactgcgta tccagcaacc
    13021 ggatggcagt tgggacctgc cggatggctt aatcgatcgc attgagtgct gtgggctgat
    13081 ggttaccgtc ggtcactggg tgctggaaga gtcctgtcga ttgcttgcag cctggcaaga
    13141 gcgcggcatt atgctgccct tgtcggtaaa cctctctgcg ctgcaactga tgcacccgaa
    13201 tatggtggcg gatatgctgg aactgttaac ccgctatcgc attcagccgg gaacactgat
    13261 tctggaagtg acagaaagcc gacgtattga cgaccctcat gctgcggtgg caatcctccg
    13321 tccgctgcgc aatgccggag ttcgggtggc gctggatgat ttcggcatgg gctacgcagg
    13381 gctgcgtcag ctgcagcata tgaaatcgtt gccaatcgac gtactgaaaa tcgacaaaat
    13441 gtttgttgaa ggcttgccgg aagatagcag catgattgct gcaattatca tgctggcgca
    13501 gagcctgaac ttacaaatga ttgccgaagg cgtggagact gaagcacaac gagactggct
    13561 ggcaaaagcg ggcgttggta ttgcccaggg cttccttttt gctcgcccac tccctattga
    13621 aatcttcgaa gagagttacc tggaagaaaa gtagctaccc caaactgatt acaaaacttt
    13681 aaaaagtgct ggtttgtgcg agccagctca aactttttaa cctttttgtt tcaattatga
    13741 tccaggtaca tttctgtgat gttgtctggg tgttatttta aggccgcagg tacccccccc
    13801 ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa acctctctgt
    13861 ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc cttggccatt
    13921 tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt aagctcatta
    13981 agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg ggcatggaaa
    14041 gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa attgtcagta
    14101 ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt gccggaatga
    14161 acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat caggcgaaag
    14221 accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt ggcgcatttg
    14281 ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt gcgctccacc
    14341 gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg caggtcatct
    14401 tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcgctcggg gcaatggcgt
    14461 ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg attatctgtt
    14521 tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct aaagcgactg
    14581 gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt gtactgggga
    14641 cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa ctcggctgcc
    14701 gtaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt gatggcacat
    14761 cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt cagatggata
    14821 tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg gcggcagggg
    14881 taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc catttgccgg
    14941 tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct cgtgcgctaa
    15001 ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg aaagaactgg
    15061 accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa acgcacgaat
    15121 tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg gtgtttgcgc
    15181 ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat ttcacttctt
    15241 ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc atttagactg
    15301 accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca catgcagggc
    15361 ataaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg ctatgtgcag
    15421 gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa cggtttacag
    15481 tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg cctgctggtt
    15541 aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc catccctcgt
    15601 attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt gtggcagcag
    15661 gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac cacgctgttt
    15721 aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc ttatctggca
    15781 aatgccactg gcaaattgac catcacacca gaaaccatca accacgcgct gcaaagtcag
    15841 gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta tcgtctgaaa
    15901 gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt tgaagcggaa
    15961 aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct actggtggtg
    16021 ggaaacgtgg atgcgcgctc ggttgtcgac caaatcaaca aaacgtttgg cgaactgaaa
    16081 ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc ggaagcggtg
    16141 agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga tacgccgtgg
    16201 cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct ggcccgcgag
    16261 gcgctgttct ggcatgttca gcaagcgtta agcgccagtg acagcaaaga catcggtctt
    16321 ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat cgaatcacca
    16381 aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa ggttcgcgat
    16441 aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact ggagctgcag
    16501 aaactgtttg ccgcctatgc acgggctgat accgatattc tgatgggcca gcggatgcgt
    16561 tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact gcggcaggat
    16621 ttccttaaca gcctgactgt ggagatgtta aatcaggatc tgcgtcagca gttgtcgaat
    16681 gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat gaaagcgttg
    16741 caggcggcct gggatcaaat catggcccca tctaccgccg ctgcgaccac ctctgtcgcc
    16801 acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg cctgatggtg
    16861 atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggggt aacactttat
    16921 ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat aatcgcgccg
    16981 cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt ttccgcactg
    17041 ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc tgcggtggta
    17101 tcgatcactt tttctttcgg cagtttcacc gccggaacat caactaacgc ttcaccagca
    17161 atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac gcccgcgtta
    17221 tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc ttcgtcgtcc
    17281 agcgtcagga aggcgatatc cgtgcattcc agcatttgct ggtacacctg ctgtgtctct
    17341 tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt tccgcctttg
    17401 gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct taagatcgcc
    17461 aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca aatcgccgca
    17521 gactgctcgc tcgccagcca gaatttggcg gcggcttcgt tacgccagta gtagaacgta
    17581 cgttcgccgg tgctgtcggt ttcaatgtag taaaggcccg gaagacggtt ttccatccgt
    17641 tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatctg ctggctaaaa
    17701 ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc gacctgacgg
    17761 gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac gtccgcgcct
    17821 ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga catagtcgtg
    17881 aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg gtcaaccata
    17941 ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga ttccgttgtg
    18001 gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttacgcgc cgataatctt
    18061 tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata aggcaggtta
    18121 tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg cgtttttggt
    18181 tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcggg cggttaatgg
    18241 ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc ctgccgccgg
    18301 atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa gagcagattg
    18361 atttgctggc gcaaaaagcc gacttctttg tagagcacgg cctgctggca tcggtcaata
    18421 ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc cagattgagc
    18481 gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa gattcaacct
    18541 ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggtacc gggatggcaa
    18601 atttctctgc actaagtgaa gtgcgttatg actacatcaa aatcgcgcga gaactgtttg
    18661 tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta catctaatga
    18721 atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag tggcgtgatg
    18781 ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg gcaccgatgg
    18841 aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc gggctggact
    18901 atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag gcaggcaaaa
    18961 taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc attttgattg
    19021 caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct gcggagttga
    19081 atcgtccgtt cgctatccgt ggcaatctgg gcgtggtgtg ggagcggcaa aaacaagaaa
    19141 ctggctggcg cagctgggtg ccgtggcctc atgtacatgc agaagacatc attcttggta
    19201 atccaccgga tattcccgaa gtcacgatgg tgcatttgcc gcgagtagag gcaacgctgg
    19261 caccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc gaaaagcccg
    19321 acgcgcgcct gattcgcctc tctgaaaaga acaataactg gacgtttaat cttgccaacg
    19381 atgacaacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg gataatattc
    19441 ttttcgatca agggcggatc gccattgatg acaaagtaag caaagcggat ctggaaattt
    19501 ttgttgatcc gttaggcaag ccgctgccat tcagcgaagt tactggatcg aaaggtaaag
    19561 cggataaaga aaaggtgggc gattacattt ttggcctgaa ggcgcaggga cgttataacg
    19621 gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt ggcgaaggga
    19681 cgccgtttcc ggtacaggct gatttccgtt caggtaatac ccgtgttgct tttgatggcg
    19741 tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt tctggcgatt
    19801 cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg ccgtttgaaa
    19861 cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt gattatcgcg
    19921 gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac accaccggaa
    19981 agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg gcggacctgg
    20041 gaccgttgat tggcgttgat tccgggaaag gggcagaaaa gtcgaaacgg tctgaacaga
    20101 agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac cgcttcgaaa
    20161 ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc attgagcatg
    20221 gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat gctgacctgc
    20281 gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat attcatctgg
    20341 aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga ctgaaactga
    20401 aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg aacggtgacg
    20461 cggaactacg cggtagcggt aactcggtgg cggcgctttt aggcaacagt aacggcaacc
    20521 tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt gttgggctga
    20581 atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg gtgaactgcg
    20641 cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt gctttcgata
    20701 ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa cagctggatt
    20761 tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg ccgctgtatg
    20821 tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cggaccgctg attgcccgtg
    20881 gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta ctggcactga
    20941 tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag atgaagaagt
    21001 gatgataatt gccggatgtc tgacatccgg caattaaaca gatttagatt tttggcaaag
    21061 tgtgctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa atccttgtga
    21121 attcaatata ttgtatggaa attcagtcct gataagcgta gcgcatcagg cttttcacac
    21181 ttattacaac gactgatgtc gcgtctcatg ggtcagcagc agggcgatta acgtcaagcc
    21241 agccatcgcc gccagatata accccaccgc acctaacccg tagttagcct gcaaccaggc
    21301 tgcgatatac ggcgcaacgg aagccccgag aatcgacgat acgttgtaag agaacgatgc
    21361 tccggtgtaa cgcacttctg tcggaaacag ctctggtaac agcgcaccca tcggcccgaa
    21421 ggtcagcccc atcagactta accccagcag caggaaggca aaaaccagaa tcgggttgcc
    21481 agaaccgagc agcgggttaa aggcgaacag cgcgaacagg atgatcagcg tggtgatgat
    21541 caccatgctt ttacggcgac caaaggcatc agccagtaat ccggcgactg gcaccatcac
    21601 gccaaaacca ataactgcca tcatcagcat ccacaacact tcgttacgcg gcaggccaag
    21661 cccaactggc gcggcggcgg tactgaaggt catagagtag accgtcatga tgtaaaacag
    21721 cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat gtttggtcag
    21781 cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct caaacaccgg
    21841 cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg ccgagaagat
    21901 aaatggcaca cgccagcccc agctcataaa ctgctcatcg gtcagtagcc aggaaagcag
    21961 caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cctagctgtg ggaaggagcc
    22021 atacagtgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg cgccgcccca
    22081 ttcaccgcct aagcccagac cctgaccaaa tcgagccagc gccagcagca gtggggcgaa
    22141 aataccaatc gtggcatagc ccggcagcag accaatcacc acggtcgaaa tccccatcgt
    22201 tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat gaccaaaaac
    22261 ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg actgtagcgt
    22321 tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa cggccgcagt
    22381 ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg cgacaaggac
    22441 tttattacgc gagttgatcg gcgtgtattc ttgttcgtgg tcgagtgttg tggctgttgc
    22501 ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac agcaaaagtg
    22561 atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaac caggcaaaaa ggggataatg
    22621 tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa tagtggataa
    22681 aactggtttc aggttaaata aaagttacca actggattta tatgctgggg ttatgagttg
    22741 gagtgaaatt gtttgctccc aaatagatgg ctgaagaggc tctggggaag agtttcaaga
    22801 ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc ctacggggag
    22861 ccaggccgaa taaaatcgta tgccggataa ggcgtttacg ccgcatccgg cctattttaa
    22921 ggctgcgttt tccctggcat tcgcgggtcg tctttatatt cggcggtggc aatccacgcc
    22981 gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg ccatcagccc cagcacggaa
    23041 ccaaatgctg cgccagaggg ggatttcatc agcgatggca gggtgtaggt catcacgatt
    23101 ttaatcactt caaaaccaat agcagccaga aatgttccgc gaatcagggc ttttttgcgc
    23161 ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa gcagatagtt ggcgaagatg
    23221 gaaatcgcca ggccaatcaa tcgccacgtc ggtttcagcc actcaatgct gttcaggtgc
    23281 agggcgctaa taatcatttg ctgcgccgaa ccggcaaccg aggtgatcga aagcgtcaca
    23341 atcagcgcaa tcaacaaacc aatcagtgaa ataaaatcac gcagatattt tacccagaac
    23401 ttctcctgat cttgcggcga gcgttcccag acatcgcgcg actgggcacg aatcgcttca
    23461 cgcaggttac ccatccagtt gatgccggaa taaagtgcca ccgccaggcc gacaagccct
    23521 acagtcgtac gctgctgaac ggcggtgttg atggtgtttt tcaacgtggc ggctagtgtc
    23581 ggatcgctga tgttttgcag aattttgtcg aagatatcct gtagcaacat cggatgggag
    23641 gccagcacaa agccccctgc ggcaaacgac accatcaaaa tcgggatcat cgacaaaaat
    23701 gagaaatagg tgatagccgc accaaactgg ttacccagcc gatcgttaaa gcgttctgtc
    23761 gcgcgaataa ggtgcgcaat aaccggttgg cgttggactt tttcggcagt ggtggtgacg
    23821 gtttccagcg cctggctaac tttgccgcct ttctcgctat tatccaactg cttaatcggc
    23881 tcgtgctcca gatcctgggt gggacgtttg atctcgtttt cctgcgtcat ccgctctttt
    23941 gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc agtccaggtg ctctttcatt
    24001 acgccagcca gccactgcat aaacaggttt acacgccggg aaagctcccg acgctgcggg
    24061 taaaccagcg aaagggagag cggctcggca cggtagcctg gcaacacttc aataagccgc
    24121 ccggcacgca gggcttcgcg cacggcaatg cgcgggatct gaataatccc cagaccggca
    24181 agaccggcgg cgagatagtt ttcgctactg tttaccgtca acatgccgcc agacttaaac
    24241 cactggacgc cattaacgct ggcaacctca aaacctaacg gatgtacacc caggtgcggt
    24301 gtataacgca ctattgcgtg tgaagtcaga tcatcggggc tttgcggata accaaagcgc
    24361 gtcagatagt gcggactggc acagttgacc atggttagtt tgccgagggg acgggcgata
    24421 acgccatctt ccggtaacgc gccagtgcgt atcacgcaat caaaaccatc atgaagaata
    24481 tctaccggac ggttatggct actcagttcc agctcaattc ccggatgctg atagagaaat
    24541 tccgacagac gcggcagtaa caggtttttc gcgattcctg acgggatgtc gatgcgtaat
    24601 ttaccgctga tactggtggc atcctgttga aacagaccgt ccagttcgct gagattactc
    24661 aacacatctt ttgcccgttg ataataggtc atgccttctg gcgtgagttt gacccgtcgc
    24721 gtagtgcgct ggagaagctg ggtgccaagt tgatgttcca gtgcctgtat ctggcgcgaa
    24781 acacttccct ttggcaaagc aaagaaatcc gccgcgcggg aaaaactttc cagctccgcg
    24841 actttgatga acaactgcat tgcgtgaatt ttatccattt ctttgaccga ttgttgttca
    24901 cagcgaaaca gtaacgcgca tatcgcattg tttattgttt tttctaacat ctaatgccgg
    24961 gtttcacaat gttgtcatga cgaatgtggc gatttctcag caggaaaagg gcggaatagc
    25021 tttatgagaa aggtgaatcc ccttaaacag agcgtatttt tcagtgattc cctttaaagt
    25081 gaatagagga cgtattggct atttgtgctt atttttgtct cattgccatt tgttattttg
    25141 gcttgtagtc gcggaatttc atttttaatt gcatgcgtac taaccaccaa tataaggaaa
    25201 tcctcaaaat tgttttaaat agtgccctcg acatgtaatt tcaggcgttt agctgtaact
    25261 atttatatcg gtaactattt tctacgcgtc atctctgtag aatggccacc gccaaatggc
    25321 gaattcttat tttttctcgc gggtaatacc cttccactac ttaagtcttt ttaacgtatt
    25381 caggtggaga ttcagttatg caaatagtca tgtttgacag gcagtcaata tttattcatg
    25441 gaataaaaat cagtttacag cagcgtattc caggagtgag tattcagggg gtcagtcagg
    25501 cagacgagtt atggcaaaag ctggaaagtt accctgaagc cttagttatg ctcgatggcg
    25561 atcaggatgg tgagttttgc tactggttgc tgcaaaaaac cgtggtgcag tttcctgagg
    25621 ttaaggtgtt aatcacggcg acggattgca ataaacggtg gttacaggaa gttatccatt
    25681 ttaatgtgct ggccattgtg cctcgcgatt caaccgtcga gacatttgcg ttggcggtaa
    25741 atagtgcggc gatggggatg atgtttctgc ctggagactg gcggacgacg ccggaaaagg
    25801 acattaaaga tctcaaatcg ttaagcgccc gccagcggga gattttaacc atgttagcgg
    25861 cgggcgaatc aaataaggag atcggcagag cgctgaatat cagtaccgga acggtaaaag
    25921 cgcatctgga atccctgtat cgccgtctgg aagtgaaaaa tcgcacccag gcggcaatga
    25981 tgttaaatat ctcctcctg
//
LOCUS       DADLEE010000058        25668 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000058 DADLEE010000000
VERSION     DADLEE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.058"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQH51_002492"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQH51_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY6076797.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /locus_tag="JQH51_002493"
     CDS             1794..2540
                     /locus_tag="JQH51_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY6076798.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="JQH51_002494"
     CDS             2630..3391
                     /locus_tag="JQH51_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY6076799.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWDVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="JQH51_002495"
     CDS             complement(3442..4326)
                     /locus_tag="JQH51_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY6076800.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQH51_002496"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQH51_002496"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY6076801.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="JQH51_002497"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="JQH51_002497"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY6076802.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="JQH51_002498"
     CDS             6202..6474
                     /locus_tag="JQH51_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY6076803.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="JQH51_002499"
     CDS             6844..7920
                     /locus_tag="JQH51_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6076804.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="JQH51_002500"
     CDS             7947..9326
                     /locus_tag="JQH51_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076805.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="JQH51_002501"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="JQH51_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11254
                     /locus_tag="JQH51_002502"
     CDS             10409..11254
                     /locus_tag="JQH51_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY6076806.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVREVRKAVKERALEKIELFGS
                     DGKAE"
     gene            11259..12206
                     /locus_tag="JQH51_002503"
     CDS             11259..12206
                     /locus_tag="JQH51_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY6076807.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12216..13196
                     /gene="ydjG"
                     /locus_tag="JQH51_002504"
     CDS             12216..13196
                     /gene="ydjG"
                     /locus_tag="JQH51_002504"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY6076808.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13333..14091
                     /locus_tag="JQH51_002505"
     CDS             13333..14091
                     /locus_tag="JQH51_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6076809.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14209..15567
                     /locus_tag="JQH51_002506"
     CDS             14209..15567
                     /locus_tag="JQH51_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076810.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYSLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15660..16301)
                     /gene="pncA"
                     /locus_tag="JQH51_002507"
     CDS             complement(15660..16301)
                     /gene="pncA"
                     /locus_tag="JQH51_002507"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY6076811.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16312..17328)
                     /gene="ansA"
                     /locus_tag="JQH51_002508"
     CDS             complement(16312..17328)
                     /gene="ansA"
                     /locus_tag="JQH51_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY6076812.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17495..19351)
                     /gene="sppA"
                     /locus_tag="JQH51_002509"
     CDS             complement(17495..19351)
                     /gene="sppA"
                     /locus_tag="JQH51_002509"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY6076813.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19512..20063
                     /locus_tag="JQH51_002510"
     CDS             19512..20063
                     /locus_tag="JQH51_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY6076814.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20180..21223
                     /gene="selD"
                     /locus_tag="JQH51_002511"
     CDS             20180..21223
                     /gene="selD"
                     /locus_tag="JQH51_002511"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY6076815.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21228..23189
                     /locus_tag="JQH51_002512"
     CDS             21228..23189
                     /locus_tag="JQH51_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY6076816.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23316..24356
                     /locus_tag="JQH51_002513"
     CDS             23316..24356
                     /locus_tag="JQH51_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY6076817.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24473..>25668)
                     /gene="gdhA"
                     /locus_tag="JQH51_002514"
     CDS             complement(24473..>25668)
                     /gene="gdhA"
                     /locus_tag="JQH51_002514"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY6076818.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6522 a         5972 c         6626 g         6548 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctgggacg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat
    11221 tgaactgttc ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt
    11281 gtataggtgc cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg
    11341 tagattctta tcctcttgaa agaatcgcaa tgagcaccgg gggcgatgca ataaatgaag
    11401 caacaattat ttctcgtctg gggcatcgta ccgcattaat gagccgcatt ggtaaagatg
    11461 cctcagggca atttattctc gaccattgtc gcaaagagaa tattgatatt caaagcctga
    11521 aacaggacgt cagcatagat acatctatta acgttggact ggtgacggag gatggcgagc
    11581 ggacgtttgt cacgaaccgt aacggcagtt tgtggaaatt aaatattgac gatgttgatt
    11641 ttgcgcggtt tcctcaggcg aaattattat cactggccag tattttcaac agtcctctat
    11701 tggatggtaa agcgctaaca gaaattttta cgcaagcgaa agcccggcag atgatcattt
    11761 gtgccgatat gatcaaaccg cggttgaatg aaacgctgga tgatatttgc gaagcattga
    11821 gttatgtcga ttatctgttt ccaaattttg ccgaggaaaa attactcaca gggaaagaga
    11881 cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa
    11941 cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg
    12001 caggaataac cgccatcgac accattggcg cgggcgataa ctttgcttca ggttttattg
    12061 cggcactgtt agaaggcaaa aatctgcgtg aatgcgcacg ctttgccaat gcaacggcgg
    12121 ctatctcggt tctaagcgtc ggtgccacca ccggcgtaaa aaacagaaag ctggtcgaac
    12181 aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacga
    12241 cggatataac gctttcgcga atggggttgg ggacatgggc cattggcggc ggtcctgcat
    12301 ggaatggcga tctcgatcgg caaatatgta ttgatacgat tcttgaagcc catcgttgtg
    12361 gcattaatct gattgatact gcgctaggat ataactttgg caatagtgaa gttatcgtcg
    12421 gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg
    12481 tctgggaacg aaaaggaagt ttattcaaca aagttggcga tcggcagttg tataaaaacc
    12541 tttccccgga atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt
    12601 acatcgatat ctatatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg
    12661 aaactgtcgc agtgcttaat gagttaaagg ctgaagggaa aattcgcgct ataggcgctg
    12721 ctaacgtcga tgctgaccat atccgcgagt atctgcaata tggtgaactg gatattattc
    12781 aggcgaaata cagtattctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg
    12841 ataatggcat tgtggttcag gtttattccc cgctagagca gggattgttg accggcacta
    12901 tcactcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa
    12961 acatgctgaa agtgattgat atgcttgaac agtggcagcc actttgtgct cgttatcagt
    13021 gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc
    13081 ttagtggggc tactgcaccg gaacaggtac gcgaaaatgt cgcggcactg aatatcaact
    13141 tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac
    13201 tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaatagg
    13261 ttattttgaa agtaaacatc ggttatgcga taatcgcgct aatgtgatgt aaaaagatac
    13321 aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac
    13381 gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt
    13441 cgccgcgatc ttgagaagct ggaagatgaa ggctttttga ccagaaccta tggtggtgct
    13501 gttttaaata cagcggtgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt
    13561 tatgaagaga agctgcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc
    13621 atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt
    13681 gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt
    13741 aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttccctgca aggaagaatt
    13801 actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt
    13861 gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg
    13921 atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgtaaagct
    13981 tttgtccagt tagctgactt tagtcatatt aattatataa taactgataa atcaccgggt
    14041 gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac
    14101 acgttgtcaa tattatttgt tcctgcgact ggaaataaaa tgatgataaa gatgcggcaa
    14161 gtgaatttgc cgcaattgct accttttata gcaaatccga ggcacccaat ggaacaatat
    14221 gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt
    14281 ggtataataa gctttagtct gttattaacg gggtttttga gttactcagg taatgtcgtc
    14341 ttagcaaagc tggtaagcaa tggatggtca aataatttcc tctatgccgc ctttacctcg
    14401 gcattaatgt ttggttattt catcggctca cttactggtg ggtttattgg tgactacttt
    14461 gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgctgc aacaggggcc
    14521 gcttttgtcc ctgatatgta ctggctcatt ttctttcgct tcctgatggg aacaggaatg
    14581 ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt
    14641 ggaaaatggt ccgcgcggct ctcatttgtt ggtaactggt cgcccatgct gtctgcggcg
    14701 ataggcgtgg tggttatcgc tttttttagt tggcgaataa tgtttcttct gggtggtatt
    14761 ggcatactgt tagcctggtt tctctcaggt aaatacttta tcgagtcgcc acgatggctg
    14821 gaagggaaag ggcaaatcgc cggtgcagaa agccaacttc gtgaagtaga gcagcaaatt
    14881 gaaagggaga agagtattcg tttaccccag cttactttga accagagcaa cagcaaggtt
    14941 aaggtaatca agggtacctt caggctcctg tttaaagggg aaatgttacg acgtacatta
    15001 gttgcgataa ctgttttaat tgcaatgaac atttcgcttt ataccattac cgtatggata
    15061 ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt
    15121 attatgattg gtgctccggt aggaatattt attgcggcat taattattga tcattttcct
    15181 cgtcgattat ttggctccgc cttacttatt attattgccg tgttaggcta tatctattca
    15241 agtcagacta cagagtgggc gattttaatc tatagtctgg tgatgatctt ctttttatac
    15301 atgtatgttt gcttcgcgtc ggcggtttat atcccggagc tttggccaac gcatttacgc
    15361 ctgcgcggtt cgggtttcgt taatgccgtc ggacggatcg tcgcagtttt cacgccctat
    15421 ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc
    15481 atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg
    15541 ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcatcaatt gccagatgta
    15601 ccactgacgt gttttatttg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat
    15661 tacccctgcg tctcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatc
    15721 tccataaacg cgtgtgcact gtcctggggc tggatattca cgccacgaca gccatcggta
    15781 atcacgttta ccttataacc taactgtaac gcgtccagca cggtaaactt cacgcaatag
    15841 tcagtagcca ggcccataac gatcaattca tcgatttcat gatcgcgcaa ccagtcatca
    15901 agcgcggttt tctgccgacg tccgttatca aaaaaggcac tgtaactgtc aactaaagga
    15961 ttttcgcctt tatggaacac cgctgcgatc tctttttgat tcagtaacgg atgtaattgt
    16021 gcgccttcac tgttctgcac acagtgttca ggccagaaag tttgccgcag gccgccgagt
    16081 tggcctggcg tataaggctc tacgccgtgc tggctggcaa agctgccgtg attcgccgga
    16141 tgccagtcct ggctggcgat aaccgcttct ccgcgcgact ggcaccagtc aataaggcgg
    16201 ttagcaacct ccaccgtact gtcaccttcc ggtacggcga gcgcgccacc agcacagaaa
    16261 tcattttgta aatcgaccag taacagggcg cgatggggca ttgcagtctc cttaatcatc
    16321 cggcgtcagt tcgccgcgca ggttttggct catggccttg cgaatggttt cagtatccag
    16381 ttcctggctc agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc
    16441 gtcaataacg ccggcatggg cgagggcgtt accggtggca taaccactca tattcacttt
    16501 accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa
    16561 ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccataggagc gcagaatcaa
    16621 tgctttcacc ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ctggataaat
    16681 cgtcaccacg ccaattggtt gtggcgtgat tggatgaacg attaattccc cttcaccgtg
    16741 cggggcgggt ggcgtattca aacgacgaat atggatacca gcttccagta acggaggaag
    16801 gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtagtgc ggttgccgcg
    16861 atacaatcgg ttattgaaaa agagcgttac ttcgttgatc ggataattcg gcgcaacgta
    16921 caacgcattc agcagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga
    16981 ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagcg cagaggcggt
    17041 atacgccatc gtgtcggtac cgtgtagaat gacaaaacca tcatagtcgt catagtgcgc
    17101 tttaatatct tcagcaatat gctgccagtc ttccggcgtc atatcagaag aatccatcaa
    17161 cggcgtatat tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag
    17221 cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat
    17281 cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg
    17341 aatgatatcg aaagagcaaa agtatatacg tgaggcagga ggagcgcacc tgatactatg
    17401 cggataagga aatatggggc gagacagcgg gaagaggatt cgtcgaggta aaaaataatg
    17461 tggccgacga tctgtcggcc actcagtaca agacttaacg cacgttggcg caggtcaggc
    17521 aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa
    17581 cggtagaggc taccgaggca agcggtgcag gtaacatggc ctggaacgca tctggcaaca
    17641 ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa
    17701 cgtagtattc cagatgccac tgtttcactt ttgcgagctc tgctgctttg gcaaccgcat
    17761 catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga
    17821 cgtggccctg ggcgatttta tcaatttgct ccggcgtcga atgacgcgca tcagcaacca
    17881 gcgtgataaa gcgtttatag ccattctcaa tgcttaattg catcatctgc tgcgcttccg
    17941 gcggcagtgc cctggtgata gaaacatccg ccagcggtga agttgagaca ccatcagtat
    18001 gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag
    18061 aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggcgtggaa atccagtaac
    18121 caccagatgc cgccatgccg cccatcgata caaccacaga cttacccgct gcccgggctg
    18181 ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgcctggg ctattaacac
    18241 gcaggacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg
    18301 tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga
    18361 cgacaccgat gctgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga
    18421 tggcgcgata atttttatca gtcttactcc agccgaactc tttggtcaga gctttttcga
    18481 tttcggcact cgatgccagt gcatcgacca gcttgttttc cagtgcatat ttcgcggtat
    18541 cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag
    18601 cagggatctg ccggttagcg gcaacagtat tcagatagtt ttgccacagc tcaccaatcc
    18661 agcggctgtc agcttcgcgg gctgccggtg acatatcatc acgaataaac ggttcaacgg
    18721 cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta tccagcaacg
    18781 atttgtagta cagaccgttg gtagcaaaac cgtgcagatc aaccacgcct tgcggtgaca
    18841 gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa
    18901 cggcataaac cggtttcccg ctgtcacgaa actctttcag ggctttgccg atgtactgca
    18961 tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacgataccg gtgatattgc
    19021 ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac
    19081 gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtctg
    19141 gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt
    19201 tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagtacg aggaaaataa
    19261 agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa
    19321 atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt
    19381 cacaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg
    19441 cggactgtgc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcacgacag
    19501 gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg
    19561 ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc
    19621 cggaccataa gtccatgcaa ccgtggcatt tttttgtgat tgaaggggaa gggtgcgagc
    19681 gtttcagcgc cgtactggaa cagggggcga ttgctgccgg tagtgatgat aaagctatcg
    19741 acaaagcccg taatgcgccg ttccgcgcac cgctcatcat cacggtggtg gcgaaatgca
    19801 aagaaaatca taaagtcccg cgatgggaac aggaaatgtc tgccggatgc gcggtcatgg
    19861 cgatgcaaat ggcagccgtt gcccagggat ttggcggcat ctggcgcagt ggcgcattaa
    19921 ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt
    19981 ttctctacct cggcacgccg cagttgaaag catctacgtc gattaacgtc ccggacccga
    20041 cgccgtttgt aacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc
    20101 gcaggaattc agacactctc acttatcact tcacggaatg agggttacca tacccggatt
    20161 gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg
    20221 agctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca
    20281 ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt
    20341 gtacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt
    20401 tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatcttcgc
    20461 gatggggggc aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc
    20521 cccggaaatt gcccgcgaag taaccgaagg tggacgctat gcatgccgtc aggcgggtat
    20581 tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac
    20641 ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact
    20701 gttcctgacg aaaccgctgg ggatcggcgt tcttaccacg gctgagaaaa aatcactgtt
    20761 gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc
    20821 gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggct ttggtctgct
    20881 gggccacttg agcgaaatgt gtcagggggc tggtgtgcag gcacgcgtcg actatgaagc
    20941 gatcccgaaa ctccccggtg ttgaagagta cattaagttg ggcgcagtac ctggcggcac
    21001 tgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga
    21061 tctgctgtgc gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga
    21121 aaatgaggtt aaaactacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact
    21181 ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taatttaatg cggttgttta
    21241 ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcccaaa ccgcaccgga
    21301 aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacttggtgt atcggtcacc
    21361 tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg
    21421 atttgccgat tgtcccggaa aagtggcaat tacagccacg accctccgtg accaaacaac
    21481 ttaacgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggggacccgg
    21541 atcgtgaagg gcaattgctg gtggatgaag tgctggacta tctgcaactg gcaccggaaa
    21601 agcgccagca ggtgcagcgt tgcttgataa acgacctgaa cccgcaggcg gttgagcggg
    21661 cgatcgaccg tcttcgttcc aacagtgagt ttgtgccatt gtgcgtttct gcgctggcgc
    21721 gagcgcgtgc cgactggctg tacggcatca atatgacccg tgcgtatacc attctcggtc
    21781 gcaatgccgg ttatcagggc gtactttccg tgggacgcgt gcagacaccc gtgctcgggc
    21841 tggtggtgcg ccgcgatgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca
    21901 aagcacatat cgtgacacct gccgatgagc ggtttaccgc tatctggcaa ccgagcgaag
    21961 cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg
    22021 tggttaaccg cattagtggt caaccggcta ttgtcaccag ctataacgat aaacgggaat
    22081 cagaatccgc gccgctgccg ttttcgcttt cggcgttgca gattgaagcg gcaaaacgct
    22141 tcggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc
    22201 taatcactta tccgcgttcc gattgtcgct atttgccaga agaacatttt gccggacgcc
    22261 acgcggtgat gaatgccatc agcgttcatg caccggatct gttgccgcag ccagtggtag
    22321 atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca
    22381 tcccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc
    22441 tgattgcccg tcaatacctg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta
    22501 tcgaactgga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag
    22561 gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccattgc
    22621 ctgtggtggc gaaaggcgat gagttgctgt gtgaaaaagg tgaagtggta gagcggcaaa
    22681 cccagccgcc gcgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcgc
    22741 gctttgtgca ggacaaagat ctgaaaaagg tccttcgtgc gaccgatggt ctggggacag
    22801 aagcaacccg tgccgggatt attgaactgc tgttcaagcg tggttttctg accaaaaaag
    22861 ggcgctatat ccactccacc gacgccggaa aagcgttatt ccattcgctg ccagaaatgg
    22921 cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc
    22981 agtgtcgcta tcaggacttt atgcagccgc tggtggggac gctatatcag cttattgatc
    23041 aagccaaacg tacgccggtg cggcagtttc gcggcattgt ggctccgggc agtggtggca
    23101 gtgctgataa gaaaaaggct gcaccgcgta aacgtagtgc gaaaaaaagt ccgccagcag
    23161 atgaagccgg aagcggggcg atagcgtaag cgagcgaatc tttcgtgcta ttcgagtcat
    23221 attctgaaat atccagcgga tcaagaaagt tcgttggata tttttttgca tggataaaat
    23281 tatcgcctct aaagtatgta ataacaggga atgtgatgaa aaaagtttta ttgcaaaacc
    23341 atcctgggag cgagaagtat tcttttaatg gctggggaat atttaatagt aattttgaac
    23401 gggtgattaa agaaaataag gccatgctgc tttgtaagtg ggggttttat ttaacatgtg
    23461 ttgtcgctgt aatgtttgta ttcgcagcga taacatccaa cggtttgaat gaaagaggcc
    23521 tgattaccgc gggatgctct tttctttatc tattaattat gatggggctt attgttcggg
    23581 ccggttttaa agcaaaaaaa gaacaactgc attattatca ggctaaaggt attgagccgc
    23641 tcagtatcga gaagttacag gcgctacaat tgatcgcacc ttatcgattc tatcataagc
    23701 aatggtctga aacgctagag ttctggccgc gaaagcctga acctggcaaa gataccttcc
    23761 aatatcatgt gcttcctttt gactcgatcg atatcatcag taaaagacgc gagtctttag
    23821 agaatcaatg gggtatcgaa gatagcgaaa gttattgtgc cttaatggag cattttcttt
    23881 ctggcgacca tggagccaat acctttaaag caaacatgga ggaagcccca gagcaggtta
    23941 tcgccttgtt gaataaattt gctgtttttc cctcagacta tatctctgat tgcgctaata
    24001 atcgctccgg taaatcctcg gcgaagctaa tatgggcggc ggaattatca tggatgatct
    24061 cgatatcaag cacagctttt caaaacggga caattgaaga agaactggcc tggcattata
    24121 taatgcttgc ttctcgaaag gcgcacgagt tgttcgaaag cgaagaagat tatcaaaaaa
    24181 atagtctaat gggatttctt tactggcata tctgctgcta tcgcagaaag ttaacggatg
    24241 ctgaacttga agcgtgttat cgttacgaca agcagttttg ggagcactac agtaagaaat
    24301 gccgttggcc cataagaaat gttccctggg gagcatcatc cgttaaatac tcataaacgc
    24361 ctgaaatttt gccgggggcg ctgcgcttga ccggcctaca ttagagcgta actgcaatga
    24421 attgtgccca tctgtaggcc tgataagcgt agcgccatca ggcatttaca acttaaatca
    24481 caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc gcgccctgca
    24541 cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca
    24601 tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt
    24661 gtgccatttc caggcccgac gttgctacac cgccagcatt agccgcttta cccggtgcaa
    24721 acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatatgccc
    24781 cttcagcgac ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt
    24841 cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc tgttggcctt
    24901 cgagatagac cagaccaaat tctttggcgt aatctgccgc tcgaccatcg cggctggctt
    24961 tgatttcgat aagacgtgcc agtttctctt tcgtgaatcc gctttcatca actacagtgc
    25021 cgctggagtc tgacgcagtg atcacacgag caccaaattc catcgctttt tcgatagcgt
    25081 actgggcgac gttgccggag ccagaaacgg aaacgctcat cccttcaaaa cccataccgt
    25141 ggcgttttaa cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa
    25201 taagactacc gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga
    25261 gctttttcat catccccgcc ataaagccga cttcacgacc accaacgccg atatcacctg
    25321 ccggaacgtc ggtatccgcg cccaggtggc gatacagttc agtcatcagc gtctggcaaa
    25381 aacgcatcac tttaccttcg ctttttcctt tcggatcgaa atcgctgccg cctttaccac
    25441 cgcccatcgg cagggtagtc agggcatttt tgaaggtttg ttcaaagccg aggaatttga
    25501 gaatggaaag gttaactgac ggatggaagc gcataccgcc tttgtacggg ccgatggcag
    25561 agctgaactg cacacgccat gcacggttga cctgtatctg gttgcgatca tcaacccata
    25621 ccacgcgaaa ctggatcacg cgctccggtt caaccagacg ctccagta
//
LOCUS       DADLEE010000059        24653 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000059 DADLEE010000000
VERSION     DADLEE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.059"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..386)
                     /locus_tag="JQH51_002515"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="JQH51_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="JQH51_002516"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="JQH51_002516"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY6076819.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="JQH51_002517"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="JQH51_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY6076820.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="JQH51_002518"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="JQH51_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY6076821.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="JQH51_002519"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="JQH51_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY6076822.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     gene            4210..4254
                     /locus_tag="JQH51_002520"
     CDS             4210..4254
                     /locus_tag="JQH51_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY6076823.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="JQH51_002521"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="JQH51_002521"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY6076824.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="JQH51_002522"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="JQH51_002522"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY6076825.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="JQH51_002523"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="JQH51_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY6076826.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="JQH51_002524"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="JQH51_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY6076827.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="JQH51_002525"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="JQH51_002525"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY6076828.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="JQH51_002526"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="JQH51_002526"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY6076829.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="JQH51_002527"
     CDS             10748..11212
                     /locus_tag="JQH51_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6076830.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="JQH51_002528"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="JQH51_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY6076831.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="JQH51_002529"
     CDS             12236..13672
                     /locus_tag="JQH51_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY6076832.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="JQH51_002530"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="JQH51_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY6076833.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="JQH51_002531"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="JQH51_002531"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY6076834.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="JQH51_002532"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="JQH51_002532"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY6076835.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="JQH51_002533"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="JQH51_002533"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY6076836.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20502)
                     /gene="fadK"
                     /locus_tag="JQH51_002534"
     CDS             complement(18802..20502)
                     /gene="fadK"
                     /locus_tag="JQH51_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAY6076837.1"
                     /translation="MHPTGPQLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRSIKLLSVYGSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEMAAFFSRKRVAKYKYPEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE
                     "
     gene            complement(20505..20798)
                     /locus_tag="JQH51_002535"
     CDS             complement(20505..20798)
                     /locus_tag="JQH51_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY6076838.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="JQH51_002536"
     CDS             complement(20795..22084)
                     /locus_tag="JQH51_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY6076839.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="JQH51_002537"
     CDS             complement(22140..23078)
                     /locus_tag="JQH51_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY6076840.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="JQH51_002538"
     CDS             complement(23098..23862)
                     /locus_tag="JQH51_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY6076841.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="JQH51_002539"
     CDS             24181..>24653
                     /locus_tag="JQH51_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY6076842.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5950 c         6484 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DADLEE010000060        24185 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000060 DADLEE010000000
VERSION     DADLEE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.060"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(25..597)
                     /locus_tag="JQH51_002540"
     CDS             complement(25..597)
                     /locus_tag="JQH51_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076843.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="JQH51_002541"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="JQH51_002541"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY6076844.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JQH51_002542"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JQH51_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY6076845.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JQH51_002543"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JQH51_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY6076846.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="JQH51_002544"
     CDS             complement(3559..5340)
                     /locus_tag="JQH51_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY6076847.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="JQH51_002545"
     CDS             complement(5333..7105)
                     /locus_tag="JQH51_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY6076848.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7680)
                     /locus_tag="JQH51_002546"
     CDS             complement(7135..7680)
                     /locus_tag="JQH51_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY6076849.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGVWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JQH51_002547"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JQH51_002547"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY6076850.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8783..10357
                     /locus_tag="JQH51_002548"
     CDS             8783..10357
                     /locus_tag="JQH51_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY6076851.1"
                     /translation="MLRQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALE
                     TGKQQDVLELAQLAPGELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAE
                     QHLAGQIFSGLTRFDNNTQRPIGDLAHHWETSTDRLRWDFYLRSTLHWHNGDAVKASH
                     LHQRLLMLLQLPALDQLFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQ
                     FPLIGTGPFRLTQFTAELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPV
                     QITIGKPEELQRVSQVSSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEV
                     GENLITASHALLPGWTIPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGC
                     ELTIIFHNAKNWDDTTLLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYA
                     HLQSTLDAVQVMPDEENRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTP
                     RGWFEFTEAWLPAPSQ"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="JQH51_002549"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="JQH51_002549"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY6076852.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /locus_tag="JQH51_002550"
     CDS             complement(11169..11567)
                     /locus_tag="JQH51_002550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY6076853.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="JQH51_002551"
     CDS             complement(11661..12032)
                     /locus_tag="JQH51_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076854.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="JQH51_002552"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="JQH51_002552"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY6076855.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="JQH51_002553"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="JQH51_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY6076856.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="JQH51_002554"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="JQH51_002554"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY6076857.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="JQH51_002555"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="JQH51_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY6076858.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="JQH51_002556"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="JQH51_002556"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY6076859.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="JQH51_002557"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="JQH51_002557"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY6076860.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="JQH51_002558"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="JQH51_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY6076861.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="JQH51_002559"
     CDS             21803..22381
                     /locus_tag="JQH51_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6076862.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="JQH51_002560"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="JQH51_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY6076863.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6666 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gttat
//
LOCUS       DADLEE010000061        24091 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000061 DADLEE010000000
VERSION     DADLEE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.061"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            7..180
                     /locus_tag="JQH51_002561"
     CDS             7..180
                     /locus_tag="JQH51_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY6076864.1"
                     /translation="MTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEAS
                     FSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="JQH51_002562"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="JQH51_002562"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY6076865.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="JQH51_002563"
     CDS             1533..2480
                     /locus_tag="JQH51_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY6076866.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="JQH51_002564"
     CDS             2550..3356
                     /locus_tag="JQH51_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6076867.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="JQH51_002565"
     CDS             3366..3773
                     /locus_tag="JQH51_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY6076868.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3773..4267
                     /locus_tag="JQH51_002566"
     CDS             3773..4267
                     /locus_tag="JQH51_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY6076869.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="JQH51_002567"
     CDS             4333..5130
                     /locus_tag="JQH51_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY6076870.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="JQH51_002568"
     CDS             5141..5929
                     /locus_tag="JQH51_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY6076871.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /locus_tag="JQH51_002569"
     CDS             5981..7210
                     /locus_tag="JQH51_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY6076872.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="JQH51_002570"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="JQH51_002570"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY6076873.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="JQH51_002571"
     CDS             complement(8372..10003)
                     /locus_tag="JQH51_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY6076874.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="JQH51_002572"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="JQH51_002572"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY6076875.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="JQH51_002573"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="JQH51_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY6076876.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15247..17431
                     /locus_tag="JQH51_002574"
                     /pseudo
     CDS             15247..17431
                     /locus_tag="JQH51_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="JQH51_002575"
                     /pseudo
     CDS             complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="JQH51_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="JQH51_002576"
     CDS             complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="JQH51_002576"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY6076877.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="JQH51_002577"
                     /pseudo
     CDS             complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="JQH51_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22525..23454)
                     /gene="metA"
                     /locus_tag="JQH51_002578"
     CDS             complement(22525..23454)
                     /gene="metA"
                     /locus_tag="JQH51_002578"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY6076878.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23611..24054
                     /locus_tag="JQH51_002579"
     CDS             23611..24054
                     /locus_tag="JQH51_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY6076879.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5945 a         6146 c         6109 g         5891 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatcg cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaaa agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtggaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggtgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctggtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gagtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcacacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggtggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca gattctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcatg gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgaggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgcttaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tggcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc
    15121 gaaactttta cggctgcatt atgagattga gcgatttatg atattttttt agttgtaatc
    15181 atattaaatt ttttcactgt tctgatagtt aaaattcaag acatcaataa acaatgagat
    15241 atttaaatga ttactcgtat tcctcgtagt tctttctctg caaatattaa taatacagcc
    15301 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
    15361 tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
    15421 gttcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
    15481 gctgataaga aagaaattac gatgattaat aaaaacgaaa acacgacact cactcaaacc
    15541 attgctacaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
    15601 gataaacaag aattaaacca aaaatcagat agaaaagaaa aagaattccc aagaattaaa
    15661 cttaatggtc aatgttattt cccggggcgc cccaaaaccg tatagtatac cgacacattg
    15721 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
    15781 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
    15841 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
    15901 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
    15961 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
    16021 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatta
    16081 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
    16141 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
    16201 cacttccgaa agaacagcta ttgatgttaa aaggaactgt ggatgaaata acccctccat
    16261 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
    16321 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
    16381 atagtattaa cctccccggt ttatatttgg ctataaatta tggcaatgcg gatatcgttg
    16441 agactatttt caattcattg tcagaaacag gatatgaagg gttactctcg aaaaaaaatc
    16501 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
    16561 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa
    16621 cacatcattt agataatgaa caggtgtata aattcctgag tgccaaaaat agaacgtcca
    16681 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa atttttttgg
    16741 tcgcgttacc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
    16801 tgaaggcaaa ggatttttat ggttgcccaa gactatacct ggcgatgcaa aatggacata
    16861 gcgatatcgt gaaagttatt ctcgaagcat tgccctgtct ggcccaggaa attaacattt
    16921 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
    16981 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
    17041 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    17101 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    17161 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    17221 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    17281 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    17341 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    17401 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    17461 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    17521 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    17581 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
    17641 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    17701 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    17761 agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    17821 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    17881 atccgccgct cgattataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    17941 ttccgctgct tcctggagca gtaattccat taataccggg gaaatatgcc gcttctccag
    18001 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    18061 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    18121 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aacaccaaca tcaccattcc
    18181 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    18241 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    18301 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    18361 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    18421 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    18481 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    18541 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    18601 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    18661 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    18721 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    18781 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    18841 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    18901 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    18961 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    19021 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    19081 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    19141 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    19201 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    19261 attgccggat gcggcgtaaa tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
    19321 ttagaactgc gattcttcag tggagccagt cagcgcggtg actgaagacg tgccgccctg
    19381 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
    19441 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt
    19501 cataccttcg ccctgcgcat aagcgtttgc caggtcgaac atgttaaacc acatgctgtg
    19561 gatacctgct agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
    19621 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
    19681 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
    19741 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
    19801 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
    19861 gccggtaata aattcgctgt catacgggtc gcaatctgag gtgatcagat ccgccgcatc
    19921 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
    19981 cgcgaccagt ttctgaatag cttcctgagt tggcactaaa actttgccgc ccatgtgacc
    20041 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgcagcac cggcttcaat
    20101 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
    20161 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccacggacca
    20221 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
    20281 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
    20341 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
    20401 accgccagtc agtgcaccga ggttgttgat gtagcctttt ttcgactcac cgtgcagcag
    20461 acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
    20521 taatttcacc acatcttccg cactgtatgg gcgagtaatg ccttcccaac gcggttgagt
    20581 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
    20641 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
    20701 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag
    20761 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
    20821 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
    20881 cagatcgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
    20941 acgcagccgt tgccggagat ccacgcttcg atgtactgca cagccacgcg aatgttagcg
    21001 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
    21061 ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac gggagccgag aatgtcgttg
    21121 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
    21181 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attacgctct
    21241 tcatctttgc tcggaataaa cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg
    21301 caggttttaa tcagcaggcg tgagtaagca ttcaggaatg gtttatccat cgtcacggcc
    21361 tgtctgtctg gcaggacacg atcgggatag tttttcaacg ttttgatata gctgaagatg
    21421 taatcccagc gaccgcagtt cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg
    21481 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
    21541 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
    21601 taggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
    21661 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
    21721 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
    21781 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt accgttaacc
    21841 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
    21901 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
    21961 tctaccgggc cagttatctc tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt
    22021 ttccaatcag catcgcgaat ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta
    22081 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
    22141 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
    22201 tcgccatacg gccttgtgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
    22261 cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt
    22321 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
    22381 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacttgctg
    22441 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
    22501 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
    22561 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
    22621 acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc gggaaatagt tatacggtac
    22681 atccgggtct agtccggctt ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc
    22741 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
    22801 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
    22861 cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag
    22921 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
    22981 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
    23041 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
    23101 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
    23161 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
    23221 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
    23281 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
    23341 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
    23401 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
    23461 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
    23521 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
    23581 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
    23641 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
    23701 ttatcagcag agtatcgggc cgagctggaa gagctggtgc gttcctttct gccggaagca
    23761 ccgttgtggg tcgcggttaa tgaacgggag cagccggttg ggtttatgtt gttaagtggg
    23821 cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg
    23881 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
    23941 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
    24001 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
    24061 tctgccgtgc ctgcccaagc tggagaagac g
//
LOCUS       DADLEE010000062        22412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000062 DADLEE010000000
VERSION     DADLEE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.062"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            82..2682
                     /locus_tag="JQH51_002580"
     CDS             82..2682
                     /locus_tag="JQH51_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY6076880.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="JQH51_002581"
     CDS             2673..3656
                     /locus_tag="JQH51_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6076881.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="JQH51_002582"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="JQH51_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="JQH51_002583"
     CDS             4302..4817
                     /locus_tag="JQH51_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6076882.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="JQH51_002584"
     CDS             4783..5520
                     /locus_tag="JQH51_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY6076883.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="JQH51_002585"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="JQH51_002585"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY6076884.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="JQH51_002586"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="JQH51_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY6076885.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="JQH51_002587"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="JQH51_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY6076886.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="JQH51_002588"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="JQH51_002588"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY6076887.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="JQH51_002589"
     CDS             10827..12734
                     /locus_tag="JQH51_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6076888.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="JQH51_002590"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="JQH51_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY6076889.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="JQH51_002591"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="JQH51_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY6076890.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="JQH51_002592"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="JQH51_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY6076891.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="JQH51_002593"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="JQH51_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY6076892.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="JQH51_002594"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="JQH51_002594"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY6076893.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="JQH51_002595"
     CDS             complement(17402..19162)
                     /locus_tag="JQH51_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY6076894.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="JQH51_002596"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="JQH51_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY6076895.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="JQH51_002597"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="JQH51_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY6076896.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="JQH51_002598"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="JQH51_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY6076897.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="JQH51_002599"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="JQH51_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY6076898.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5258 c         5873 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DADLEE010000063        22281 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000063 DADLEE010000000
VERSION     DADLEE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.063"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1028)
                     /locus_tag="JQH51_002600"
     CDS             complement(<1..1028)
                     /locus_tag="JQH51_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6076899.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="JQH51_002601"
     CDS             complement(1130..2110)
                     /locus_tag="JQH51_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY6076900.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="JQH51_002602"
     CDS             complement(2118..2828)
                     /locus_tag="JQH51_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY6076901.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="JQH51_002603"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="JQH51_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY6076902.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="JQH51_002604"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="JQH51_002604"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY6076903.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="JQH51_002605"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="JQH51_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY6076904.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="JQH51_002606"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="JQH51_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY6076905.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /gene="yshB"
                     /locus_tag="JQH51_002607"
     CDS             9405..9515
                     /gene="yshB"
                     /locus_tag="JQH51_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY6076906.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="JQH51_002608"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="JQH51_002608"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY6076907.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="JQH51_002609"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="JQH51_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY6076908.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="JQH51_002610"
     CDS             12397..13029
                     /locus_tag="JQH51_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY6076909.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="JQH51_002611"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="JQH51_002611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY6076910.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16199..16441
                     /locus_tag="JQH51_002612"
     CDS             16199..16441
                     /locus_tag="JQH51_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076911.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            16561..17493
                     /locus_tag="JQH51_002613"
     CDS             16561..17493
                     /locus_tag="JQH51_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6076912.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="JQH51_002614"
     CDS             complement(17534..18964)
                     /locus_tag="JQH51_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY6076913.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="JQH51_002615"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="JQH51_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY6076914.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="JQH51_002616"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="JQH51_002616"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY6076915.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="JQH51_002617"
     CDS             complement(20825..21094)
                     /locus_tag="JQH51_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY6076916.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="JQH51_002618"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="JQH51_002618"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY6076917.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="JQH51_002619"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="JQH51_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY6076918.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcactgg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacactc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgaggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DADLEE010000064        21512 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000064 DADLEE010000000
VERSION     DADLEE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.064"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JQH51_002620"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JQH51_002620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY6076919.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JQH51_002621"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JQH51_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY6076920.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JQH51_002622"
     CDS             complement(2649..3200)
                     /locus_tag="JQH51_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY6076921.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JQH51_002623"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JQH51_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY6076922.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="JQH51_002624"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="JQH51_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="JQH51_002625"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="JQH51_002625"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY6076923.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="JQH51_002626"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="JQH51_002626"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY6076924.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="JQH51_002627"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="JQH51_002627"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY6076925.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="JQH51_002628"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="JQH51_002628"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY6076926.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="JQH51_002629"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="JQH51_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY6076927.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="JQH51_002630"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="JQH51_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY6076928.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="JQH51_002631"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="JQH51_002631"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY6076929.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="JQH51_002632"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="JQH51_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY6076930.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="JQH51_002633"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="JQH51_002633"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY6076931.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQAMVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="JQH51_002634"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="JQH51_002634"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY6076932.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="JQH51_002635"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="JQH51_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY6076933.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="JQH51_002636"
     CDS             complement(18170..18565)
                     /locus_tag="JQH51_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY6076934.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="JQH51_002637"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="JQH51_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6076935.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="JQH51_002638"
     CDS             19481..20074
                     /locus_tag="JQH51_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6076936.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /locus_tag="JQH51_002639"
     CDS             20219..20671
                     /locus_tag="JQH51_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY6076937.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="JQH51_002640"
     CDS             20794..>21512
                     /locus_tag="JQH51_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY6076938.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5197 a         5324 c         5667 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccat
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DADLEE010000065        21503 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000065 DADLEE010000000
VERSION     DADLEE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.065"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JQH51_002641"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JQH51_002641"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY6076939.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JQH51_002642"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JQH51_002642"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY6076940.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JQH51_002643"
     CDS             complement(3121..3420)
                     /locus_tag="JQH51_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076941.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JQH51_002644"
     CDS             3595..4590
                     /locus_tag="JQH51_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6076942.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JQH51_002645"
     CDS             complement(4632..5069)
                     /locus_tag="JQH51_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY6076943.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JQH51_002646"
     CDS             complement(5115..5456)
                     /locus_tag="JQH51_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6076944.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="JQH51_002647"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="JQH51_002647"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY6076945.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="JQH51_002648"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="JQH51_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="JQH51_002649"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="JQH51_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6076946.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="JQH51_002650"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="JQH51_002650"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY6076947.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="JQH51_002651"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="JQH51_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY6076948.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /locus_tag="JQH51_002652"
                     /pseudo
     CDS             12687..13865
                     /locus_tag="JQH51_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13973..14797)
                     /locus_tag="JQH51_002653"
     CDS             complement(13973..14797)
                     /locus_tag="JQH51_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY6076949.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="JQH51_002654"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="JQH51_002654"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY6076950.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="JQH51_002655"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="JQH51_002655"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY6076951.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="JQH51_002656"
     CDS             complement(18729..19202)
                     /locus_tag="JQH51_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY6076952.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="JQH51_002657"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="JQH51_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY6076953.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="JQH51_002658"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="JQH51_002658"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY6076954.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21503
                     /locus_tag="JQH51_002659"
     CDS             21362..>21503
                     /locus_tag="JQH51_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY6076955.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5403 a         5490 c         5276 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcaccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgc
//
LOCUS       DADLEE010000066        21355 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000066 DADLEE010000000
VERSION     DADLEE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21355
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.066"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1461
                     /gene="caiT"
                     /locus_tag="JQH51_002660"
     CDS             <1..1461
                     /gene="caiT"
                     /locus_tag="JQH51_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY6076956.1"
                     /translation="LIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMVVM
                     LFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
                     FGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMEVIRPSSTLVPL
                     AGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIII
                     TCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLL
                     MYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMV
                     MGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVARAIIETWAALPLSTATMWG
                     FFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGG
                     LKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1492..2634
                     /gene="caiA"
                     /locus_tag="JQH51_002661"
     CDS             1492..2634
                     /gene="caiA"
                     /locus_tag="JQH51_002661"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY6076957.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2763..3980
                     /gene="caiB"
                     /locus_tag="JQH51_002662"
     CDS             2763..3980
                     /gene="caiB"
                     /locus_tag="JQH51_002662"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY6076958.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4054..5607
                     /gene="caiC"
                     /locus_tag="JQH51_002663"
     CDS             4054..5607
                     /gene="caiC"
                     /locus_tag="JQH51_002663"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY6076959.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5716..6501
                     /gene="caiD"
                     /locus_tag="JQH51_002664"
     CDS             5716..6501
                     /gene="caiD"
                     /locus_tag="JQH51_002664"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY6076960.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6507..7097
                     /gene="caiE"
                     /locus_tag="JQH51_002665"
     CDS             6507..7097
                     /gene="caiE"
                     /locus_tag="JQH51_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY6076961.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7183..7578)
                     /gene="caiF"
                     /locus_tag="JQH51_002666"
     CDS             complement(7183..7578)
                     /gene="caiF"
                     /locus_tag="JQH51_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY6076962.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7840..11061)
                     /gene="carB"
                     /locus_tag="JQH51_002667"
     CDS             complement(7840..11061)
                     /gene="carB"
                     /locus_tag="JQH51_002667"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY6076963.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11079..12227)
                     /gene="carA"
                     /locus_tag="JQH51_002668"
     CDS             complement(11079..12227)
                     /gene="carA"
                     /locus_tag="JQH51_002668"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY6076964.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(12683..13504)
                     /gene="dapB"
                     /locus_tag="JQH51_002669"
     CDS             complement(12683..13504)
                     /gene="dapB"
                     /locus_tag="JQH51_002669"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY6076965.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13671..14585)
                     /gene="rihC"
                     /locus_tag="JQH51_002670"
     CDS             complement(13671..14585)
                     /gene="rihC"
                     /locus_tag="JQH51_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY6076966.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14651..15601)
                     /gene="ispH"
                     /locus_tag="JQH51_002671"
                     /gene_synonym="lytB"
     CDS             complement(14651..15601)
                     /gene="ispH"
                     /locus_tag="JQH51_002671"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY6076967.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15603..16052)
                     /gene="fkpB"
                     /locus_tag="JQH51_002672"
     CDS             complement(15603..16052)
                     /gene="fkpB"
                     /locus_tag="JQH51_002672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY6076968.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16139..16633)
                     /gene="lspA"
                     /locus_tag="JQH51_002673"
     CDS             complement(16139..16633)
                     /gene="lspA"
                     /locus_tag="JQH51_002673"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY6076969.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16633..19449)
                     /gene="ileS"
                     /locus_tag="JQH51_002674"
     CDS             complement(16633..19449)
                     /gene="ileS"
                     /locus_tag="JQH51_002674"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY6076970.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19492..20433)
                     /gene="ribF"
                     /locus_tag="JQH51_002675"
     CDS             complement(19492..20433)
                     /gene="ribF"
                     /locus_tag="JQH51_002675"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY6076971.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20441..20659)
                     /locus_tag="JQH51_002676"
     CDS             complement(20441..20659)
                     /locus_tag="JQH51_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY6076972.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20762..21025
                     /gene="rpsT"
                     /locus_tag="JQH51_002677"
     CDS             20762..21025
                     /gene="rpsT"
                     /locus_tag="JQH51_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY6076973.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5073 a         5744 c         5492 g         5046 t
ORIGIN      
        1 ttaataatcg tcggcatact ttgttggctt acagtcagag atctggatgc ggcgaatgtc
       61 gttattaatg ctgtattcag ttacgtcacc aatgtatggg gatgggcatt tgaatggtat
      121 atggtggtga tgcttttcgg ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt
      181 ttaggtaacg aaccgccaga atttagcacc gccagttgga tctttatgat gttcgcctcc
      241 tgtacgtctg ctgccgtact gttctgggga tcgattgaga tctactacta catctccacc
      301 ccgccgtttg gcttagaacc gaactcgaca ggagcgaaag agttggggct ggcttacagc
      361 ttgttccact ggggacctct gccgtgggcc acttacagct tcctttcagt cgccttcgct
      421 tacttcttct ttgtccgcaa aatggaagtg attcgcccca gctcgacact ggtgccgctg
      481 gcaggtgaaa aacacgccaa agggttgttc ggtaccatcg tcgacaactt ctatctcgtc
      541 gccttgatct tcgcgatggg taccagtctg ggccttgcca cgccgctggt gaccgagtgt
      601 atgcaatggt tgtttggcat tccgcatacc ctgcaactgg acgctatcat cattacctgc
      661 tggattatcc tcaacgccat ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc
      721 agtgacgtgc gtagttacct gagcttcctg atgctgggtt gggtgttcat cgtcagcggt
      781 gccagcttca tcatgaacta cttcaccgat tcggtgggga tgttgctgat gtatctgccg
      841 cgcatgttgt tctataccga tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg
      901 ttctactggg catggtgggt gatttacgcc atccagatga gtatcttcct cgcccgcatc
      961 tcccgtggtc gtactgtgcg tgaactgtgc ttcggcatgg tgatggggct gacagcatcc
     1021 acctggatcc tgtggaccgt gctcggtagt aacactctgc tgttgatgga taaaaacatc
     1081 atcaacattc caaatctgat cgaacagtac ggtgtggcgc gcgccatcat cgaaacctgg
     1141 gccgctctgc cactcagcac cgccaccatg tggggcttct tcatcctctg ctttattgcc
     1201 accgttacgc tggttaacgc ctgctcttat accctggcga tgtctacttg ccgcgaagta
     1261 cgcgatggtg aagaaccacc gctgctggtg cgtatcggtt ggtcaattct ggttggcatt
     1321 atcggtattg ttctgctggc gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc
     1381 ggaggatgcc cgctgttctt cgtcaacatt atggtgacgc tctcctttat taaagacgcg
     1441 aaacagaact ggaaagatta attaaccccc aaaatatcaa gaggttgaaa gatggatttt
     1501 aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa
     1561 aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc
     1621 aaagcactgg cggatatggg tatcgacagt ctgctgatcc ctgaagagca cggtggtctg
     1681 gacgcggggt ttgttactct cgccgccgtg tggatggagc tgggacgtct gggggcacca
     1741 acctatgtac tgtaccagct gccgggcggg ttcaatacct tcctgcgcga aggcacacaa
     1801 gagcagatcg acaagattat ggctttccgc ggcaccggta agcagatgtg gaactcagcg
     1861 attaccgaac cgggcgcggg ctccgacgtg ggtagcctga aaacgactta tacccgtaga
     1921 aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagtagcgc ctacaccccg
     1981 tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg
     2041 tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgagaagct cggtctgcgt
     2101 atggatagct gctgtgaaat cacctttgac gatgtggaac tggacgagaa agacatgttc
     2161 ggtcgggaag gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg
     2221 gtagccctga ccaactacgg tacggcgatg tgcgcctttg aagatgctgc gcgctacgcc
     2281 aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc
     2341 gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa
     2401 gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat
     2461 gcggcatttg aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc
     2521 aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag accgcgtttc cggaggatct
     2581 gacgaaatgc agatcctgac gctgggtcgt gcggtgctga agcaataccg ctaagtccgt
     2641 gcgctgcctg atgcgacgct gacgcgtctt atcaggccta cgggagcacg aatgtaagcc
     2701 ggataaggcg tttacgccgc atccggcagt catgcgctac ataaattttt caggagctaa
     2761 taatggatca tctacccatg ccgaaattcg gaccgctggc cggattgcgc gttgtcttct
     2821 ccggtatcga aatcgccgga ccgtttgccg gacaaatgtt cgcagaatgg ggcgcggaag
     2881 ttatttggat cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc
     2941 agctctcccg ccgcaatttg cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg
     3001 aagcgtttct gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg
     3061 cctttgcccg tcgtggcatt accgatgaag tactgtggca gcacaacccg aaactggtta
     3121 tcgctcacct gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct
     3181 ataacaccat cgcccaggcc ttcagtggtt acctgattca gaacggtgat gttgaccagc
     3241 caatgcctgc cttcccgtat accgccgatt acttttctgg cctgaccgcc accacggcgg
     3301 cgctggcagc actgcataaa gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca
     3361 tgtatgaagt gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg
     3421 aaatgtgccc gcgcatgagc aaaggtaaag atccctacta cgccggttgc ggtctgtata
     3481 aatgtgccga cggctacatc gtgatggaac tggtgggcat tacccaaatt gaagagtgct
     3541 ttaaagatat tggcctcgca catctactcg gtacgccgga aatcccggaa ggcactcagc
     3601 ttatccaccg tatcgaatgc ccttacggcc cactggtaga agaaaaactc gatgcctggc
     3661 tggcggcaca taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg
     3721 ccaaagtact gaccgtaccg gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa
     3781 tcactcagtg gcaaacgatg gatggtcgca cctgcaaagg gccgaacatc atgccgaaat
     3841 tcaaaaataa ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg
     3901 ccattttgaa aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc
     3961 tggccaaagt tgaggactaa acgcgtggcg tcagtcggct tgcctgacta gcgcccgccc
     4021 aataacaaac gaacaaaaat ggatagaggt gcaatggata tcattggcgg acaacatcta
     4081 cgtcaaatgt gggacgatct ggcggacgtt tacggtcata aaacggcgct gatttgtgaa
     4141 tccagcggcg gagtcgttaa ccggtatagt tatcttgagt taaatcagga gattaaccgc
     4201 acggcaaacc tgttttatac gctggggatt cgcaaaggca acaaggttgc actacatctc
     4261 gacaactgcc cggaatttat cttttgctgg ttcgggctgg caaaaattgg cgcgattatg
     4321 gtgccgatta acgcccgcct gttacgcgag gaaagcgagt ggatcttgca aaatagccag
     4381 gcgtgcctgc tggtgaccag tgcgcaattt tatcctatgt atcaacagat tcagcaggaa
     4441 gatgccactc aattgcggca catttgcctg acagatgtgg cacttcccgc tgatgatgga
     4501 gtgagttcat ttactcaact gaaaaatcaa caacctgcca ccttgtgcta tgcaccgccg
     4561 ctatcgactg acgatacggc ggaaattctc ttcacctccg gcaccacctc ccgaccgaaa
     4621 ggtgtggtga ttacccatta caacctgcgc ttcgctggat attactccgc ctggcagtgt
     4681 gcactgcgtg acgatgacgt ctacatgacg gtaatgcctg cgtttcatat cgattgccag
     4741 tgtactgcgg cgatggcggc gttttctgcc ggggccacct ttgtgctggt cgagaaatac
     4801 agcgcccgcg ccttctgggg acaggcgcag aagtaccgcg ccaccattac cgaatgtatt
     4861 ccgatgatga tccgtacgtt gatggtgcag ccgccttcag cgaacgatcg gcaacaccgc
     4921 ctgcgggaag tgatgtttta tctcaacttg tcggagcagg aaaaagacgc attttgtgaa
     4981 cgcttcggcg ttcgcttgct gacgtcttat gggatgacgg aaaccattgt gggcattatc
     5041 ggcgatcgcc ctagcgataa acgacgctgg ccgtcgattg gtcgggcggg gttttgctac
     5101 gaagcggaga tccgcgacga tcacaatcgc ccgctcccgg caggtgagat cggtgaaatc
     5161 tgtattaaag gcgtaccagg gaaaaccatc ttcaaagagt attttctcaa cccgaaagcc
     5221 actgcgaaag tgctggaagc cgacggctgg ctacataccg gcgataccgg ataccgcgac
     5281 gaagaaggct ttttttattt cgtcgatcgc cgctgcaata tgatcaaacg cggtggcgag
     5341 aatgtctcct gcgtggagct ggaaaatatc atcgccaccc atccgaaaat tcaggacatc
     5401 gtggttgtgg gtattaaaga ttcgattcgc gatgaagcca tcaaagcatt tgtggtgctg
     5461 aatgaaggtg aaacattgag cgaagaggaa ttttttcgct tctgcgaaca aaatatggcg
     5521 aaatttaaag tgccctctta tctggagatc agaaaagatc tgccacgtaa ttgctcgggg
     5581 aaaataatta gaaagaatct gaaataaatg aaacgtcagg gaactaccct gccagctaat
     5641 aatcacgccc taaaacaata tgcatttttt gcagggatgt tgtcgtccct aaaaaagcaa
     5701 aaatggagaa aaggaatgag tgaatcatta catctgaccc gcaatggatc aattctggaa
     5761 attacccttg atcgcccaaa agcgaatgct attgatgcaa aaaccagctt tgaaatgggc
     5821 gaagtatttc taaatttccg tgacgatccg caattacgtg tcgccattat taccggtgcc
     5881 ggagagaaat tcttttccgc gggctgggat ttaaaagcgg cagcagaagg cgaagcaccg
     5941 gatgctgact ttggcccggg tggttttgcg ggattaaccg aaattttcaa tctcgacaaa
     6001 ccagttatcg cagctgtgaa cggctacgcc tttggcggcg gtttcgaact ggcgctggcg
     6061 gcagatttta ttgtttgtgc cgataacgcc agcttcgccc tgccggaagc caaactgggc
     6121 atcgttcctg acagcggcgg tgtgctgcgt ctgccgaaga tcctgccgcc taccatcgtc
     6181 aatgaaatgg tgatgaccgg cagacgaatg ggcgcagaag aggcgctgcg ttgggggata
     6241 gtcaaccgcg tggttagcca ggcggaactg atggataacg cccgcgaact ggctcagcag
     6301 ctggttaaca gcgccccgct ggcgattgcg gcgctgaaag agatctaccg caccaccagc
     6361 gaaatgccgg tagaagaagc gtatcgctat attcgcagcg gcgtgttgaa acactatcca
     6421 tcggttctgc attcggaaga tgccattgaa gggccgctgg cgtttgccga gaagcgcgat
     6481 ccggtgtgga aaggacgtta acaaccgtga gctattacgc ctttgagggg ttaattccgg
     6541 tggttcaccc gacggcgttt gtccatccca gtgccgtctt gattggcgat gtgattgtgg
     6601 gagccggtgt ctacatcggc ccactcgcct cactgcgtgg tgactacggg cggttgatcg
     6661 tgcaagcggg agccaatatt caggatggct gcattatgca tggctactgc gacactgaca
     6721 ccatcgttgg ggaaaacggc catatcgggc atggagcgat cctgcatggt tgtgtgattg
     6781 gtcgcgatgc attggtcggg atgaacagcg taattatgga tggcgcggtc attggcgaag
     6841 agagcattgt tgccgcaatg agctttgtca aagcaggctt tagtggcgag aaacgccagt
     6901 tgttgatggg tacgccagcc cgcgctgtac gcagtgttag tgacgacgag ttacactgga
     6961 aacggttgaa taccaaagag tatcaggatc ttgtcgggcg ctgtcatgca tcgttacatg
     7021 aaacgcagcc actgaggcaa atggaggaaa atcgcccccg tttgcagggg acgacggatg
     7081 tgacgcctaa acggtgatct tgccggatgc gacctggttg ctggataaga tgctacaaca
     7141 tcgtatctgg caagcaggac gcccggtatc aacgagataa aattaacgac gcatactctt
     7201 tgacaacatc atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat
     7261 acgcggtgtc tttcttacgc cgactaattt ttcccgactg ttattgcgca ggcgcgcgta
     7321 aatttgctca tcgatatcga ccactttaac cagtcgctga cactggcatc cccgcccttc
     7381 cagcttatta gggatcatct taacttcgca gcttatttct gtgacttccg acagaatata
     7441 agtcagggta ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc
     7501 tatcacccac cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc
     7561 aacatatcct tcacacatac gaactccagt ttcacaaatt aaaagtctag ctatgactcg
     7621 ttttgaagca attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt
     7681 caccgtcgat aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa
     7741 agatttactt aatgggcaaa aaaaaacggt cctcatcaga gaaccgtatt agttattcga
     7801 tcaaattagc ggatgaaaaa tatccgccac gacacgctat tatttgatct gtgcgtgcat
     7861 ttcctgcacc gaaattactt tttcagtcgc atcggcattc agcgccatcg cggtagcgaa
     7921 accaccgttc agggtggtgt cataatgcac tttatattgc agcgcactgc gacggatcac
     7981 gcgggagtct tcaatcgcac gacggcctga ggtggtgttg atgatgtagg tatattcgcc
     8041 attcttgata cggtcctgaa tgtgcggacg gccttcatgc accttgttta ccagacgcgg
     8101 attgataccc gcttcgccca gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc
     8161 ctgtttcagc agttttgccg ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga
     8221 aagcagcgca cgaccgtgtt tcttcatggt ggagttgctg cccagctgcg ctttggcgaa
     8281 cgcttcagcg aaggtgcggc ccacgcccat gacttccccg gtagagcgca tttctggccc
     8341 taacagcggg tcaacgcctg ggaatttatt gaacggcagc accacttctt tcaccgagta
     8401 gtacggcgga ataacttctt tggttacgcc ctgctcagcc agcgatttgc cagccatcac
     8461 acgcgccgcc acttttgcca gcggtacgcc ggtggctttg gagacgaacg gaacagtacg
     8521 cgccgcacgc gggtttactt caatcaggta aacttcgttg tttttcaccg caaactgcac
     8581 gttcatcaga ccgcgcacct gcaattcgaa ggccagtttc tgcacctgct ggcgcatcac
     8641 atcctgaatt tcctgactta aggtgtacgc tggcagcgaa catgcggagt caccggagtg
     8701 cacacccgcc tgctcgatgt gctccatgat gccgccaatc agcaccattt cgccgtcgca
     8761 gatggcatcc acgtcaactt ctactgcgtc atcaaggaaa tgatccagca acactggcgc
     8821 atcgttagac acgctaaccg ccgtctggaa gtagcgacgc aggtcagctt cgtcatagac
     8881 gatttccatc gcccgaccgc cgagaacgta agacggacgc accaccagcg ggtagccaat
     8941 ctcttttgcc ttctcaactg ccatttcaat agcagtaacg gtggcgttcg ccggttgttt
     9001 cagtttcaga cggtcaaccg catgctggaa gcgttcacgg tcttccgcac ggtcgatagc
     9061 atccgggctg gtgccgataa ccggtacgcc agcggcttcc agtgcacgcg ccagtttcag
     9121 cggggtctga ccgccgtact gaacgataac gcctttcggc ttctcaatac gcacgatttc
     9181 cagcacatct tccagagtta ccggctcgaa gtagaggcga tcggaagtgt cgtagtcggt
     9241 agagacggtt tccgggttac agttaaccat aatggtttcg taaccgtctt cacgcagcgc
     9301 cagcgaggcg tgtacgcagc agtagtcgaa ttcgataccc tgaccgatac ggtttggacc
     9361 gccgccaagc accatgattt tttcacggtc ggtagacgga ttcgcttcgc actcttcttc
     9421 ataagtggag tacatgtaag cggtgtcggt ggcaaactct gccgcacagg tatccacgcg
     9481 cttgtagacc gggtgcaggt catattggtc acgcagctta cggatttccg cttcgcgtac
     9541 gcccgccagt tttgccaagc gcgcatcggc aaagcctttg cgtttcagct ggcgcaggaa
     9601 ttcagcgttc aggccagtga tgcccacttc cgccactttc tcttccagac gcaccagttc
     9661 ttcaatctgt accaggaacc agcggtcaat gttagtcagg ttgaagacgc cgtccacaga
     9721 caggcccgcg cggaaagcat cggcgatgta ccagatacgc tctgcgcctg cgtctttcag
     9781 ttcgcgacgg attttggtta acgcttccgg gtcatccagg ctcactttcg ggtcgaagcc
     9841 agttgcgccc acttccaggc cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc
     9901 aatcgccatc acttcgccaa ccgatttcat ctgagtggtc agacggtcgt tagcaccggc
     9961 gaatttttcg aagttgaagc gaggaatttt agtaaccaca tagtcgatgg acggctcgaa
    10021 ggaggccgga gtacgtccgc cagtgatgtc gttcatcagt tcgtctaggg tgtaacccac
    10081 cgccagtttc gccgccactt tagcaatcgg gaaaccggtc gctttcgacg ccagcgccga
    10141 agaacgggac acgcgtgggt tcatttcgat aacaatcagg cgaccgtttt tcgggttcac
    10201 cgcaaactgg acattggaac caccggtttc aacgccgatt tcacgcagca ccgccatcga
    10261 ggcgttacgc atgatttgat attctttgtc ggtcagcgtt tgggctggcg cgacagtgat
    10321 ggagtcaccg gtgtggatgc ccatcgcatc aaagttttcg atagagcaga cgatgatgca
    10381 gttgtcgttt ttatcacgca ccacttccat ctcgtactct ttccagccga tcagcgactc
    10441 atcaatcagc aactctttgg tcggagagag atccagaccg cgggcgcaaa tttcttcaaa
    10501 ctcttcacgg ttataagcga taccgccgcc gctaccgccc atggtaaagg atgggcgaat
    10561 aatgcacggg aagcccacgt cagcggcaac agccagcgct tcttccatcg tgtgtgcgat
    10621 accggaacgc gcggtttcca gaccaatttt cttcatcgct acgtcgaaac gacggcggtc
    10681 ttctgcttta tcaatcgcat cggcagtggc accaatcatg gtgacaccga actcttccaa
    10741 cacgccctga cgttccagct ccagcgcgca gttcagcgcc gtctgaccgc ccatggttgg
    10801 cagcaccgcg tccgggcgct ctttttcaat aatcttgcgt accacttccc agtgaatcgg
    10861 ctcgatgtag gtcgcatcgg ccatttccgg gtcggtcatg atggtcgccg ggttggagtt
    10921 caccagaatg acgcggtaac cctcttcgcg cagggcttta catgcttgcg cgccagagta
    10981 gtcaaactca cacgcctgac cgataacaat cgggcccgca cccagaatca ggatactttt
    11041 tatatctgta cgttttggca tggctctttt actcctgatt acttagcggt tttacggtac
    11101 tgctcaatta acttgataaa gtggtcgaac aacggcgcgg cgtcgtgtgg accagggctg
    11161 gcttcagggt gaccctggaa gctgaacgcc ggcttatcga tgcgatgaat gccctgtaac
    11221 gtaccgtcga acagggattt atgcgtgaca cgcaggtttg caggtaatgt tgcttcatcc
    11281 accgcaaaac cgtggttctg ggcggtgatc atcaccacgt ttttctccac atctttaacc
    11341 ggatggttgc cgccgtggtg accaaatttc attttgacag tcttcgcacc gctcgccagc
    11401 gccagcagct gatgaccgag acagatgccg aataccggaa tatcggtttc gaggaacttc
    11461 tggatggcgg taatggcgta atcgcacggg gccgggtcgc caggaccgtt ggagaggaag
    11521 atgccgtctg gattcatttt cagcacatct tccgcagaag tttgcgccgg aacgatggtc
    11581 aggcgacagc ctctatccac cagcatccgc aggatgttgc gcttggcacc aaaatcataa
    11641 gccacgacgt ggaacggcag ctcgtcttct tttttcgctt ctggcaggcc gccggtcaac
    11701 gtccagctcc cttgtgtcca gctataggct tctgcggtgg tcacttcttt tgccagatcc
    11761 atgccgttca gacctgggaa cgcgcgggct ttttctaacg ccagcgccgc atccgggtta
    11821 tcgcctgcga taatgcagcc attctgtgcg cctttctcgc gcagtaaacg cgtcagctta
    11881 cgggtatcga tatcggcaat cgccacgatg ttatggcgct tcaggtaaga agagaggtct
    11941 tcggtattac ggaagttgct ggcaatcagc ggcaggtcgc gaatcaccag accttgtgca
    12001 tgtacctgag aagattcttc atcggcgtca ttagtgccga cattgccaat atggggataa
    12061 gtaagagtaa cgatttgacg agaataggaa ggatcagtga ggatttcttg ataaccggtc
    12121 attgaagtat tgaaaacgac ttccccaacc gccgaacctg ttgcccctat ggcccgaccg
    12181 tgaaactggg ttccgtcttc cagaaccaat agcgctgact taatcaaaac accctccaga
    12241 gaatactcac tcactttatt tgcatattaa ttcacagtga tgacgtaaat caatgcaaat
    12301 ctgcttaccg acgaatttct ggcaaacggc ggcattctgg agatatgggc gctaaaagtc
    12361 aacttaatgg caggattttt tatattattt tgtggctcat gcatgtttta caggcattaa
    12421 gcgacaaaaa catcatctaa cctgttctgg aaaacgcttg cgcagcaaga agattgcatg
    12481 aaataaaaac gagcaggaaa aagtggacca aatggtcaaa atttacatta aaacaacaaa
    12541 aatagaagac gaaacatgta atttttggca gttatagaaa atttaaaagc ataacaccaa
    12601 aaataaaagg gctaaaaatt aaagcccttt ataattaata aattaaatgt gttacttttg
    12661 catcataata aacattttgt ggttacaaat tattgagatc aagcacatct cgcatatcaa
    12721 aaagaccact ttccttacca ctcaaccaca aagccgatct taccgcaccg ttagcaaatg
    12781 tcatgcggct ggacgcctta tgggtgatct ccagacgctc gccaatatcg gcaaacatcg
    12841 cggtatgttc gccaacgatg tcacctgcac gcacggtagc aaaaccaatg gtacctggca
    12901 cacgttcacc ggtatggcct tcacgactgt agaccgcgca atctttcaga tctttatcaa
    12961 gggcgtgggc gatcgcctct cccattgcca gtgcggtgcc tgacggcgca tcaactttat
    13021 gtctatgatg tgcttcaata atttcgatat cggtgtagtc acccatcact ttggctgctt
    13081 tctccagcag cttaagcatg acgttaacgc caacgctaaa attggcggca aagacaatcg
    13141 caatatcggc ggcggcgtca cgaattgcct gtttaccggc ttcgtcaaac cccgtagtgc
    13201 cgatcaccat ccctttgcca tgctgccgac aaaaagcgag atggttcagc gtaccttccg
    13261 gacgggtaaa atcgataaac acatcaaaat catcttttat cgcatcaaga ctgctttgca
    13321 cggtaacgcc tgttttccct gcaccagcca gctcaccggc gtcgctgccc agtaaagaag
    13381 atccttcgcg ctccagcgcc gcgcccagct gcacgccttc taatgccagt gccgcctgaa
    13441 tcaactggcg gcccatacgc cccccggctc ccgcgatggc aacgcggatg tttgcatcat
    13501 gcatagctat tctcttttgt taatttgcat agaccgtttt cagagtaacc agcgcatcgc
    13561 tacgccgcca gccaaaaacg ctgataatta gaaattaatg ataaacaggg agggatatca
    13621 gtgaaaggca tgagtcgatg aatgactgca tgccgataac atgtgacagg ttacggcacc
    13681 agagccagca cctcagccac ccactgttgg aagcctttta cattcagatc caatgccacc
    13741 tgtacattgg ctggtttgcc caggcaaccg tcgatatcaa ccaccgtcgt gcctgaggta
    13801 aattcgcctt gagtttctac cgcgacaaaa cacggcttga gggtgaataa gtccgggcgc
    13861 accagccagg cgatggcgca gagatcgtgc attcgcagac cgctttgcat actgccgcta
    13921 cggtagtggc taaacagggc gtggagcatt tttccggtgc gattcaactc cggtaaagta
    13981 gcgagatagt caggggttaa tattgcctga ttggtgacat ccagaccgca catgacaatt
    14041 tcgatgccgc tacggaagac acaggcagcc gcttctggat cggcagcgat attaaactcg
    14101 gcatttggtg tacagttgcc gcgaccagca gaaccgccca tgatcaccag acgacgaata
    14161 tacggtttac attccgggca ttgtaaaagt aacagcgcaa tattggttaa cgggccgatg
    14221 gccaccaggg taacgggctc tggtgcacgc atcagggcat cccgaatcgc cagaaacgcc
    14281 ggtatcccga gtggctttcg gttgtgctca acaaagtcgt agccagccat tcccgattcg
    14341 ccgtgcacag atgctgcatc acgcggtgcg cgtaccagcg gcacagcggc tccctgggcg
    14401 agcggaatct ccgcattcca gaaatgcagc agttgcaggg cattgcgggt agttttctca
    14461 accgagacat tacccgcgac ggtggtcatc agttgcaggt cgagttcggg ggcaaaaatc
    14521 gcggcggcaa tggcgacggc atcgtcaatg ccggggtcag tatcgaggaa gataggtaaa
    14581 cgcatgtttt cttcataaaa aatgccggta acaagaccgg cattttcgca taacttaggc
    14641 tgctaatgac ttaatcgact tcacgaatat cgacacgcag ctctttcggc acttcgaaaa
    14701 caatattttc ttcacggcct tccagcggaa tggcttcacc accacccaac tgctgcaaac
    14761 gtgccaccac attctgcacc agaatatccg gagccgatgc gcccgcagtc acgccgacgc
    14821 atttaacctc tttcacccac tcttcctgga tatctttcgc atcgtcaatc aaaaacgcgt
    14881 gtttgcccat acgctgggcc agctccgcca gacggttgga gttggaggag tttttcgaac
    14941 cgaccaccaa cacaacttcc gcctgttctg ccagggcgcg taccgcttcc tgacggttag
    15001 tcgtggcgta gcagatgtca tctttgcgcg gaccgacaat tttcgggaag cgtttacgca
    15061 gcgcgtcgat cacatcagac gtgtcatcca ccgacagcgt ggtttgggtc ataaaggaga
    15121 gcttctcttc gtttttgacc gtcagtttcc acacatcgtc tggcgattcg actagataca
    15181 ttcccccttc ggggttgctg tactgcccca tcgtcccttc cacttccggg tgcccggcgt
    15241 ggccgatgag aatagattct tcgccacggc gactggcgcg ggcaacttcc atatgcactt
    15301 tggtcaccag cggacaggtg gcatcaaaca ccgtcaaatc gcggcttttc gcttcgttac
    15361 gaaccgcctg agaaacaccg tgtgcggaga aaatcaggat cgcgccgtcc ggcacttcgc
    15421 taatctgctc aataaagata gccccacgct cgcgcaagct atcgaccacg tagcggttat
    15481 gcaccacttc gtgacggaca tatatcggtg cgccgtaaat ggccagcgcg ttttcaacaa
    15541 tgctgatagc gcggtctacc ccggcacaaa aaccacgcgg gttggccaac aggatctgca
    15601 tgttacgcct ccagtgccgg atcgatttcc agcacttcaa tatcaaaatg aacggtctgc
    15661 ccggccagcg gatggttgaa atcaacggta atggagtcgc cgttaatttc gcggatcaca
    15721 ccaggcatct cactgccatc cattgcggta aaaagcatga ttgcgccaat ttctggctcg
    15781 cctgcatcca taaattcacg gcgggagaag tactgaatca ggtccggtga cggcacgcca
    15841 aacgccgcat ctggctccag cgagaaggtg gttttgtcgc ccactttcag ccccagcagg
    15901 tgttgctcca gcccttcaga aagagaagca tcgcccaggc ggaacagcgc cggtttaccg
    15961 ttgttgcgag tagactcggc ggtggtgcca tcgtcgagtt ttagcgtgaa gtgcaccagg
    16021 acggcgctat tgctctgtac agattcagac atgcaggttt gctcttattt ttgaggattt
    16081 ttgccggatg cggcctacga tttcgtagca atctgtaggc cgcatccggc aggatttatt
    16141 attgtttttt cgctttggaa ggtaaaaacc cttccagcac gatcagtgcc gcaccgacac
    16201 aaatggcagt atcggcaagg ttgaaggtgg cgaagtgcca gtcgccgacg tagaagtcga
    16261 tcatatcgac aacgaagccg tgccacaggc ggtcgaacag gttgcccagc gcgccgccaa
    16321 taatcagcgc gtaagcgata ttgtttagct tctgcgtggc cttcgagcga tacatcatca
    16381 ctgccaggag cacgctaata ccaatcgcaa taccggcaaa gaaccaacgc tgccagccgc
    16441 cgctatcggc aaggaaacta aacgccgcgc catagttacg cgcataatgc agattaagcg
    16501 acgggaacag cgggaccgta tcccccagag caaagttctg gaggatcagg tatttgctgc
    16561 ccagatcgat aatcagcacg actaccacca gccacagcca gcgtagccct gttgaacaga
    16621 tcgattgact catcaggcaa acttacgttt ttcaccgtca ccggcgacgt tgctgacaca
    16681 gcggccgcag atttctgcgt gttccgccac cttgccgaca tcctgggtgt agtgccagca
    16741 gcgtgggcac ttctcacctt cggctttact caacgcgact ttcagccctt tgagtacttc
    16801 gctctgctga gcatcagcag gtgcgtcgtt atagtctgca acggtagcgc cggaggtcaa
    16861 caggacaaat cgtaattcat cgcccagcgc ggtcagtttc gccgccagtt ccggttctgc
    16921 atacaaggtt actgccgctt ccagcgagcc acccactttc ttgtcggcac gcgcttgctc
    16981 aatgactttg ttcacttcgc cacgcacttt caacagctcg tcccagaacg catcgttcat
    17041 tgcttcactg tctgccagac caaacaggcc ttcgtaccac tcaccggtga agacgtattt
    17101 ttcacgttcg cccggcagat agccccacac ttcatcagcg gtgaaggaga ggattggtgc
    17161 catccagcgc accagcgctt cagcgatgtg atacagcgca gtctggcagc tacgacgcgc
    17221 cacgctatcc gctttggcgg tgtactgacg gtctttgatg atgtcgaggt agaaggaacc
    17281 catctcaacg gagcagaagc gcatcagacg ctgtaccact tcgtggaaat cgtatgcttc
    17341 gtacgccttg aggatgtctt cctgtgccgc tttcgcacaa cctacggccc agcgatccag
    17401 taccaccatc tcttccggtt tcaccatatc ttttgctgga tcaaaaccat tcaggtttgc
    17461 cagcaggaag cgcgcggtgt tacggatacg acgatagcta tcggcagcac gtttcaggat
    17521 ctcgtcagaa acggccattt caccggtgta gtcggttgat gccacccaca gacgcagaat
    17581 atccgcgccc agtttgttca tcacatcctg cggcgaaacg gtgttgccga tggatttaga
    17641 catcttgcga ccctgaccat ccacggtaaa gccgtgggtc agtacctgac gatacggcgc
    17701 tttgcctttc atcgcggtgg agatcattag ggaagacatg aaccaaccac ggtgctggtc
    17761 agaaccttcc agatacatgt ccgctgcgtg accggcaaat tccggacgca cgtcaacaac
    17821 agaagagtgg gtagatccgg agtcaaacca tacgtcgaga gtatccggca ctttcacata
    17881 ctggtcagct tcgtcgccga ggatctcttt cgcatcgaga tcccaccacg cctggatgcc
    17941 gtcaacttca acgcgtttag ccacttcttc catcagttcg agggtacgcg gatgcagctc
    18001 ttccgtgtct ttgtgcacga acagtgacat cggtacgccc caggtgcgct gacgggagat
    18061 acaccagtca ggacggttag caaccatcga ctcgatacgc gcctggcccc agtccgggag
    18121 ccactgcacg cctttgatct ctttcagtga ctgcgcacgc agacctttct gatccatgct
    18181 gacgaaccac tgcggtgtcg cgcggaagat gatcggcgtt ttgtgacgcc agcagcacgg
    18241 atagctgtgc tgcattttct caacgtgcag cagcgcgcct ttttcctgca gcagcgcaac
    18301 gacgatgtcg ttcgctttga agacgttcac gccatccagc gtcggataag tgcccggcag
    18361 ataagtgccg tccgggccaa ccgggttagc ggtttccagg ccgtatttct gaccgatcac
    18421 atagtcgtcc gggccgtggc caggcgcggt gtgaacggca ccggtaccgg catccagggt
    18481 aacgtgatcg ccgaggattg ccggaacgtc gaagcccata aacggatggg taaagcgcag
    18541 cagctcaagc tctgcacctt ttaccgtgcc gagaatggtg taatcggtca cgccgatacg
    18601 ctgcattacg ctttcaacca gatctttcgc cagaatcacg gcctgaccgt cgatctgcac
    18661 cagcgcatag tcgaaatcag gtgcaataga gattgcgcgg ttcgcaggca gagtccacgg
    18721 cgtagtggtc cagatcacca gcgagattgg gccgttaacg ttgcttacgc caaatttcgc
    18781 tttcagcgca tcctgatcga ccgcctggaa agcgacgtca atggacggag aagttttgtc
    18841 gtaatactca acttccgctt ctgccagtgc agaacggcag tctacgcacc agtgcaccgg
    18901 cttcgcgcct ttgtgcaggt gaccattgcc gatgattttg cccagcgcgc ggatgatgtt
    18961 ggcttcagtt ttgaagtcca tagtcagata cgggtgcgac cagtcgccca gcacgcccag
    19021 acggataaag tctttgcgtt gaccgtcaac ctgggtcgcc gcgtattcgc ggcacttggc
    19081 gcggaactcg gcggcggtga atttctcacc cggcttaccg tattcttgct ctactttcag
    19141 ctcgatcggc agaccgtggc agtcccagcc aggcacatac ggcgagtcat aaccggaaag
    19201 ccctttggac ttcacgataa tgtctttcag aatcttgtta accgagtgac caatatgaat
    19261 gctgccattc gcataaggag ggccatcatg cagaatgaag gtttttttgc cttttttagc
    19321 cgcacggatg atgccgtaca gatcatcatc agtccaacgc gccagcattc ccggttcgcg
    19381 cttggcgaga tcgccacgca tcgggaaccc tgtttccggc aaattcaggg ttgatttata
    19441 gtcactcatc agattctcgg ttccgtgttt cggtttgatt acataacagg cttaagccgg
    19501 ttttgttagc ccaaaaaatt cgcgggcggt taattcatca cgcgcaatct gcgctttcag
    19561 ttcgtccagc gacgcaaatc gctgctcatt gcgtattttt ttacgcagca ctacttgtat
    19621 atggcgaccg taaaggtcca ttgcaacatc taacaaatgc acttccagct gctggcgaat
    19681 accggcaacc gttgggcgtg ttccgatgtt tgccacgccg ggtaacggct tttcaccgag
    19741 gcccagcact tctaccgcat aaaccccttt caccggggaa acctgacggc gtagcggtac
    19801 attcgccgtc gggaaaccta tagtgcgccc taattcatca ccgtggacta cacgcccgga
    19861 gatggcaaac gggtgcccca gtaaactttc tgccagagcc agattgtcat cagccagcgc
    19921 ctgacgcacg gcggtgctgc tgatacgcac accaccttcg caaaaagttt gcgtactggt
    19981 gatatcgaag ccgtattcca tgccagcttt ctgtaataac aagaaatcgc cttcacgacc
    20041 agcgccaaag cggaaatcat cacctacggc aagaaatttt acgcgcaaat gcttcaccaa
    20101 aagatcgctg atgaaatttt gcgcggttaa cgccgcaaaa cgcctgtcga aacgcacgca
    20161 cagcacgtaa tcaacgccac actctgcaag gtaacgcagt ttttcccgca gccgggtcag
    20221 tcttgccggg gctttatcgg tagcaaacag ttccagtggt tgaggttcaa aaagcatcac
    20281 catcaccggt aagttgcgct tgcgcccttc ttcctgcaag ccctgtaaca gcgcgcgatg
    20341 accgcgatgc acgccgtcga aattaccaat agtcagcaca cacccttctt gcggggcctg
    20401 gctgagatta tgtatgccgc gtatcagctt catgtctggc tcaaaacagt gaaaatcgtc
    20461 cgagtatacc ttgtacagcg gtcaaggtta accggcgatt gagtaccgaa atcagaaaga
    20521 agtggctttt cattgccatg gagcaaatca cgggaagaaa ctgaccgcct gctgcaattt
    20581 ttatcgcgga aaagctgtat tcacaccccg caagctggta gaatcctgcg ccatcactac
    20641 gtaacgagtg ccggcacatt aacggcgctt atttgcacaa atccattgac aaaagaaggc
    20701 taaaagggca tattcctcgg cctttgaatt gtccatatag aacacatttg ggagttggac
    20761 cttggctaat atcaaatcag ctaagaagcg cgccattcag tctgaaaagg ctcgtaagca
    20821 caacgcaagc cgtcgctcta tgatgcgtac tttcatcaag aaagtatacg cagctatcga
    20881 agctggcgac aaagctgctg cacagaaagc atttaacgaa atgcaaccga tcgtggaccg
    20941 tcaggctgct aaaggtctga tccacaaaaa caaagctgca cgtcataagg ctaacctgac
    21001 tgcacagatc aacaaactgg cttaatcgcc aatttgctga agctttgtga aaaagcccgc
    21061 gcaagcgggt ttttttatgc ctgctgcttt tgtgatgcgt gagagaacac actgccggtt
    21121 gccagatagt caaaagagac atgccgttgt actatctgaa ctcttcgtgg cttaagaatc
    21181 gtagaaccat cggctgatac attctgaaca agaatatcta ctcaatctcc agactccccc
    21241 acaaagaata tggatattgt gatacacatt gaggtattaa acttaagacc actctatttt
    21301 tgaattaaat tcctatatac tttaaccatt cataaatatc tcctcattag acctt
//
LOCUS       DADLEE010000067        21275 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000067 DADLEE010000000
VERSION     DADLEE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.067"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..444)
                     /locus_tag="JQH51_002678"
     CDS             complement(<1..444)
                     /locus_tag="JQH51_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY6076974.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /locus_tag="JQH51_002679"
     CDS             complement(499..1350)
                     /locus_tag="JQH51_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY6076975.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /locus_tag="JQH51_002680"
     CDS             complement(1363..2163)
                     /locus_tag="JQH51_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY6076976.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQH51_002681"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQH51_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY6076977.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="JQH51_002682"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="JQH51_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY6076978.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="JQH51_002683"
     CDS             complement(5214..6812)
                     /locus_tag="JQH51_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY6076979.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQH51_002684"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQH51_002684"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY6076980.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="JQH51_002685"
     CDS             complement(8491..9069)
                     /locus_tag="JQH51_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY6076981.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQH51_002686"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQH51_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY6076982.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="JQH51_002687"
     CDS             10508..10687
                     /locus_tag="JQH51_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY6076983.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="JQH51_002688"
     CDS             complement(10807..11166)
                     /locus_tag="JQH51_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY6076984.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="JQH51_002689"
     CDS             complement(11528..11872)
                     /locus_tag="JQH51_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY6076985.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="JQH51_002690"
     CDS             12004..13914
                     /locus_tag="JQH51_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY6076986.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="JQH51_002691"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="JQH51_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY6076987.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="JQH51_002692"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="JQH51_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY6076988.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="JQH51_002693"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="JQH51_002693"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY6076989.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="JQH51_002694"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="JQH51_002694"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY6076990.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="JQH51_002695"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="JQH51_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY6076991.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20053)
                     /locus_tag="JQH51_002696"
     CDS             complement(19451..20053)
                     /locus_tag="JQH51_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY6076992.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(20410..20976)
                     /locus_tag="JQH51_002697"
     CDS             complement(20410..20976)
                     /locus_tag="JQH51_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6076993.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="JQH51_002698"
     CDS             21188..>21275
                     /locus_tag="JQH51_002698"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6076994.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5273 c         5465 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DADLEE010000068        21257 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000068 DADLEE010000000
VERSION     DADLEE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.068"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            21..794
                     /locus_tag="JQH51_002699"
                     /pseudo
     CDS             21..794
                     /locus_tag="JQH51_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="JQH51_002700"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="JQH51_002700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY6076995.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="JQH51_002701"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="JQH51_002701"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY6076996.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="JQH51_002702"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="JQH51_002702"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY6076997.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /locus_tag="JQH51_002703"
     CDS             complement(4992..7226)
                     /locus_tag="JQH51_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY6076998.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="JQH51_002704"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="JQH51_002704"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY6076999.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="JQH51_002705"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="JQH51_002705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY6077000.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="JQH51_002706"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="JQH51_002706"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY6077001.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="JQH51_002707"
     CDS             complement(12981..14321)
                     /locus_tag="JQH51_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY6077002.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /locus_tag="JQH51_002708"
     CDS             complement(14323..15675)
                     /locus_tag="JQH51_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077003.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="JQH51_002709"
     CDS             complement(16109..16558)
                     /locus_tag="JQH51_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY6077004.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="JQH51_002710"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="JQH51_002710"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY6077005.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="JQH51_002711"
     CDS             complement(17358..17687)
                     /locus_tag="JQH51_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY6077006.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="JQH51_002712"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="JQH51_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY6077007.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="JQH51_002713"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="JQH51_002713"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY6077008.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="JQH51_002714"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="JQH51_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY6077009.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5671 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgacaag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccatcat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctccc
//
LOCUS       DADLEE010000069        20779 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000069 DADLEE010000000
VERSION     DADLEE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.069"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            49..579
                     /locus_tag="JQH51_002715"
     CDS             49..579
                     /locus_tag="JQH51_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6077010.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRAKEARCGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JQH51_002716"
     CDS             597..1235
                     /locus_tag="JQH51_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6077011.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JQH51_002717"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JQH51_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY6077012.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JQH51_002718"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JQH51_002718"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY6077013.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JQH51_002719"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JQH51_002719"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY6077014.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JQH51_002720"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JQH51_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY6077015.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JQH51_002721"
     CDS             complement(5552..6613)
                     /locus_tag="JQH51_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY6077016.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JQH51_002722"
     CDS             complement(6610..8007)
                     /locus_tag="JQH51_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY6077017.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JQH51_002723"
     CDS             8163..9161
                     /locus_tag="JQH51_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY6077018.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="JQH51_002724"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="JQH51_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="JQH51_002725"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="JQH51_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="JQH51_002726"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="JQH51_002726"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY6077019.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="JQH51_002727"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="JQH51_002727"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY6077020.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="JQH51_002728"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="JQH51_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(15296..16071)
                     /locus_tag="JQH51_002729"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="JQH51_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="JQH51_002730"
     CDS             complement(16089..17141)
                     /locus_tag="JQH51_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY6077021.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="JQH51_002731"
     CDS             complement(17172..18014)
                     /locus_tag="JQH51_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY6077022.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="JQH51_002732"
     CDS             complement(18001..18882)
                     /locus_tag="JQH51_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY6077023.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="JQH51_002733"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="JQH51_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /locus_tag="JQH51_002734"
     CDS             complement(20200..>20779)
                     /locus_tag="JQH51_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY6077024.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5072 a         5667 c         5051 g         4989 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtt gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctagtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DADLEE010000070        20712 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000070 DADLEE010000000
VERSION     DADLEE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.070"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQH51_002735"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQH51_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY6077025.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQH51_002736"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQH51_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY6077026.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQH51_002737"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQH51_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY6077027.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQH51_002738"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQH51_002738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY6077028.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JQH51_002739"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JQH51_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY6077029.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JQH51_002740"
     CDS             complement(8217..9110)
                     /locus_tag="JQH51_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY6077030.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JQH51_002741"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JQH51_002741"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY6077031.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JQH51_002742"
     CDS             complement(10007..10189)
                     /locus_tag="JQH51_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY6077032.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JQH51_002743"
     CDS             complement(10241..11473)
                     /locus_tag="JQH51_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY6077033.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JQH51_002744"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JQH51_002744"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY6077034.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JQH51_002745"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JQH51_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY6077035.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="JQH51_002746"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="JQH51_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="JQH51_002747"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="JQH51_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY6077036.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="JQH51_002748"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="JQH51_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY6077037.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="JQH51_002749"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="JQH51_002749"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY6077038.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /locus_tag="JQH51_002750"
     CDS             complement(19828..20592)
                     /locus_tag="JQH51_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY6077039.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DADLEE010000071        20700 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000071 DADLEE010000000
VERSION     DADLEE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20700
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.071"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="JQH51_002751"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="JQH51_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY6077040.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="JQH51_002752"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="JQH51_002752"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY6077041.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="JQH51_002753"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="JQH51_002753"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY6077042.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2436..3296
                     /gene="pdxY"
                     /locus_tag="JQH51_002754"
     CDS             2436..3296
                     /gene="pdxY"
                     /locus_tag="JQH51_002754"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY6077043.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="JQH51_002755"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="JQH51_002755"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY6077044.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="JQH51_002756"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="JQH51_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY6077045.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="JQH51_002757"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="JQH51_002757"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY6077046.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /locus_tag="JQH51_002758"
     CDS             complement(6799..7494)
                     /locus_tag="JQH51_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY6077047.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="JQH51_002759"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="JQH51_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY6077048.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="JQH51_002760"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="JQH51_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY6077049.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11211)
                     /gene="rsxC"
                     /locus_tag="JQH51_002761"
     CDS             complement(9181..11211)
                     /gene="rsxC"
                     /locus_tag="JQH51_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY6077050.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11204..11782)
                     /gene="rsxB"
                     /locus_tag="JQH51_002762"
     CDS             complement(11204..11782)
                     /gene="rsxB"
                     /locus_tag="JQH51_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY6077051.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11782..12363)
                     /gene="rsxA"
                     /locus_tag="JQH51_002763"
     CDS             complement(11782..12363)
                     /gene="rsxA"
                     /locus_tag="JQH51_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY6077052.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12440..12880)
                     /locus_tag="JQH51_002764"
     CDS             complement(12440..12880)
                     /locus_tag="JQH51_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY6077053.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(12966..13181)
                     /gene="ydgT"
                     /locus_tag="JQH51_002765"
     CDS             complement(12966..13181)
                     /gene="ydgT"
                     /locus_tag="JQH51_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY6077054.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13454..13579)
                     /gene="blr"
                     /locus_tag="JQH51_002766"
     CDS             complement(13454..13579)
                     /gene="blr"
                     /locus_tag="JQH51_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY6077055.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13822..14862
                     /locus_tag="JQH51_002767"
     CDS             13822..14862
                     /locus_tag="JQH51_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY6077056.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14898..15899)
                     /gene="add"
                     /locus_tag="JQH51_002768"
     CDS             complement(14898..15899)
                     /gene="add"
                     /locus_tag="JQH51_002768"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY6077057.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16003..17175)
                     /gene="malY"
                     /locus_tag="JQH51_002769"
     CDS             complement(16003..17175)
                     /gene="malY"
                     /locus_tag="JQH51_002769"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY6077058.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17185..18777)
                     /locus_tag="JQH51_002770"
     CDS             complement(17185..18777)
                     /locus_tag="JQH51_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY6077059.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            18952..19980
                     /locus_tag="JQH51_002771"
     CDS             18952..19980
                     /locus_tag="JQH51_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY6077060.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20092..>20700
                     /gene="hdhA"
                     /locus_tag="JQH51_002772"
     CDS             20092..>20700
                     /gene="hdhA"
                     /locus_tag="JQH51_002772"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY6077061.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5298 a         5207 c         5305 g         4890 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc
     9481 attagcctgt tgctgttcca gcttgcgcgc tttggagcgg gcgatagctg cttcaacggc
     9541 agttttacgt ggttcagcaa cggttgctgc gtcgttagtt tgctccagtt ctgcctgttt
     9601 cgctctggct tgcgctttac gggcttcccg ttctgcaata attgcactgt tatccgggcg
     9661 ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc tgtttctctt tcacccgcgc
     9721 cagagcggca gcaatcgcat ctttatcttt ggctgcaggt tgaacggctg cgctcttatg
     9781 tcgttcaagg cgagtcgctt tttcgcgctc cagacgagcc tggcgcgctt cgaaacgcgc
     9841 tttggcttct gcggcgcgct tttcttcctg acgaatagcc gcaatttcag ctttttcctg
     9901 acggaaatat tgcaccaagg gaatattgct cgggcaaacc cacgcgcaag ccccacattc
     9961 aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt tgctgacctt tgctgaacca
    10021 gtacaactgt tgcggcaaaa gatccacagg gcaggcgtca gcacaggcgc tacaccggat
    10081 gcagctttgt tcttcctgtg gttcgccaag ttcattggca gagggagcca acagacagtt
    10141 ggtaatcttt acgaccggga catccagcca tggcaaggta aagcccatta gcgggccacc
    10201 cataatcacc atttgatcgg cagaggggca gaatccggca tcattcaata aatgacgcac
    10261 tggcgtcccc agccgtgccc agacgttgcc cgggcgagcg attgcttcgc cagtcagggt
    10321 tacaacacgc tcggtaatcg gctcgccatc aataacggca cgtttcactg cataagcagt
    10381 gccgacgttt tgcattaata cgccgatatc ggatgaacgc ccgccatgtg gaacctgctt
    10441 cccggtcaga atgtaggtta attgtttagc accgccagaa ggatatttgg ttggaatcac
    10501 ccgcagagaa atatcgttag agtccgccag caccgcgcgc agcatggaaa tcgcctgcgg
    10561 tttgttatct tcaatgccga taagaatttc gcgtggctgc agaatatgcg caagaatgcg
    10621 aataccctct acgacctgag ccgcgcaatc ctgcatcaaa cggtcatcgg cggtaatgta
    10681 cggctcgcac tcagcagcgt tgataatcaa cgtttcaatc ttatctccac caccctgcaa
    10741 tttaacgcct gtcgggaatc cggcaccgcc cagcccggca acaccaaact gatgtatgcg
    10801 ctcgattaac tcttcgcgac tgcgagagcg ataatcgacc cagccgtcgc gcgggatcca
    10861 gcagtcttca ccatcggcat caataatcac gcttaattca gctaaagctg aaggatgagc
    10921 cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg ggcgcgtgaa caggcagcat
    10981 tttgccgcga ccacgggtaa gcggctggcc gcgcaatact ttatcgccga cgctaacgca
    11041 caactcacct tcagcgccaa tatgctgttt cagtggaata acaaaatgct gcgccagtag
    11101 tacctggcgc aggggggtac cgttggtctg ggttttcatc tccggtggat ggatgccgcc
    11161 gttgaaatcc cagattttat tttttctgaa tgcagagaat aacttaagca tggtgttcca
    11221 cgggaatgat acgcacggga atggtgttca gatcccattt ccaggagtca ggtgtttctg
    11281 cgaccggttg caacgagatg cagtgcgtcg ggcacggatc aacacataaa ttgcagcccg
    11341 tacagagatc actcattacc gtatgcatga cacgggtagc gccaacgatg gcgtctaccg
    11401 gacaagcctg aatacattta gtgcagccaa tacagttatt ttcatcaata accgccacca
    11461 tccgcgcagg cgttagctct tgcgcttcgc catccagcgg ctgcggctcg acattaagca
    11521 actcggcaat ttttagcatc acagcttcgc cacctggggc gcaacggttg attttttcac
    11581 cgttacagct gatggcttcc gcgtaggggc gacagccggg ataaccgcac tgaccacact
    11641 ggctctgcgg taagatttcg tcaattttct caacgaccgg atcgtcttcc accgcaaaac
    11701 ggcgggaggc ataacccaga atggcgccaa acgccaggcc cagcaggctc acggcggcaa
    11761 cggcaatcca gatagcattc attacaactt caccaaacca ctaaagccca taaaggccag
    11821 agacataaga cctgcggtaa ttaacgcaat ggcattaccg cgaaaaggtg ccgggacatc
    11881 agccacagca aggcgttcgc ggatggcggc gaagagcacc atcaccagcg agaaaccgac
    11941 agcggcggaa aaaccgtaca gcgccgactg caagaaattg tgcccgagat tgatattcag
    12001 caacgccacg ccgagcactg cacagttggt ggtgataagc ggcaaaaaaa tccccagcag
    12061 acggtaaagc accgggctgg ttttgcgcac caccatctcg gtgaactgca cgaccacagc
    12121 aatcaccaga ataaatgcca gggtgcgcag gtaaatcaga ttaagtggga tcaaaatcca
    12181 cgtatcgata agccaggcgc aaatagacgt cagcgtcatc acaaacgttg ttgccagccc
    12241 catgcccatc gcggtttcca gttttttgga aacccccata aacggacaga gaccgagaaa
    12301 cttgaccagt acaaagttat tgaccagtac agttccgaca aacagtaaca ggtagtcagt
    12361 catttttcgg cctgaaataa aaaagccgcc tattatccgt taatccagag caggcgacaa
    12421 caggttaact gtaaggttat tacggattca caaacgtcgc ttttacccgc gacgagcgtt
    12481 tgaaataggg gacgatcaat gcggttgcca acaaagtaaa cagcaactgg cgaacggcta
    12541 tgccaacttc cacgggtgaa aaagcgaatg cttttactgc cagcaatacg gaaataagca
    12601 gccagataat atagtgttta ggaacacaac gccgacgttt aaagaaagcg atggtcaacc
    12661 acagtgtgta ataccacatg gcgatagcgg taataaaaga aacgccccat aagatttgtg
    12721 tcgtcagtgc ttgtccgccg agcgtttgaa atgtttgcgg agaagataac gcagcggtat
    12781 acagcaacaa cgccagcgtc gtactcaaca gagcgactaa aagccaggcc aacgggccaa
    12841 gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat aggttctcca gaatagcgac
    12901 gcaaacaggc aaatctaatt ttaggggcgt tattataagg cttttataac cgatcgctac
    12961 tttctttatt ggacatagtg ccagacggac ttcggtacct ggccgaggtc aaacaaacgc
    13021 ccgccagaaa ccagctctgc gcgacggtga tcggcagcac gatacatatt gatcagttcc
    13081 tgatcgtccg tcagggtgta attaagatgg tcgtagagtt tttccagact ttcgagtgaa
    13141 ctgattttgc gaaattttaa taagtagtcc tgaacagtca taaaaggtcc ataaaataat
    13201 taataccaaa ggggtaggta ttattagtga tgataaaatt tcaccaaaag tgaacgcttg
    13261 tgttattaca ataataagaa tgcgctgatg gcaaatattc agggatgaaa aactgctctg
    13321 aaaagagtaa cgcgctttac tatccctgac aatcaccaac aacatcgaac aagataataa
    13381 attcctggtt taatatccga caagtgaaaa catgcacccg gacgggcagc atgtcgctcc
    13441 acaagtgcag agcttacttg tgttgtaccg aagcactctg ttcaggtggc tgatagttgt
    13501 caatgtgact cgccacgcca agaagaatga ctgaaacgac aagaacgatc caacctgtta
    13561 attcaataag acgattcatt acagcccaca tctctcttga ttgatccatt aacttcaggg
    13621 ggtaaatgtt acttagcaat aatagctcca gcaagatttt tactgaggtt tttgcgatat
    13681 ttagcttttg tcgttggaaa attcgctatt tttttgactt aagttaaaca acatccctta
    13741 ttgctggcag gttattaaac tgttgagcgt gggtaaagga tagtgtcaaa tagccatcat
    13801 acttcaatga gaggcaatga catgagcgac aacatccgtg ttgggttgat tgggtatggt
    13861 tatgcgagca aaaccttcca tgcgcccctg attgcgggca cgcccgggct ggaactggcg
    13921 gtaatctcca gcagtgatga aacaaaagta aaagccgact ggccaacggt tacggttgtc
    13981 tctgagccga agcatctgtt taacgatccc aacatagacc tgattgtcat tcctacaccc
    14041 aacgataccc atttcccgtt agccaaagcg gcgcttgagg cgggtaaaca tgtggtcgtt
    14101 gataaaccct ttaccgtgac actgtcacaa gcgcgagagc tggatgcgct ggcaaaaagc
    14161 ctggggcgtg tgctgtctgt attccataac cgccgttggg atagcgattt cttgacgcta
    14221 aaaggtttgc tcgcggaagg cgtgctgggt gaagttgctt actttgagtc tcattttgac
    14281 cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg gcggcccagg cagcggtatc
    14341 tggtacgatt tagcaccgca tcttcttgat caggccatta cgctatttgg tttaccggtc
    14401 agcatgacgg tagatttggc acagttacgg cccggagcgc agtcgaccga ttatttccac
    14461 gccatcttgt cctatccaca gcggcgagtc attttacacg gtaccatgct ggcagccgct
    14521 gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt atgtgaaata tggcctcgat
    14581 ccacaggaag aacgtctgaa aaatggcgag cgtctgccgc aggaagactg gggctacgat
    14641 atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg
    14701 acggtgcctg ggaattatcc ggcttactat gcggcgattc gtgatgcgtt aaatggcgat
    14761 ggtgaaaatc cggttccggc aagccaggca atccaggtaa tggagttgat tgagctgggc
    14821 atcgaatccg ccaaacatcg cgcgacgttg tgccttgcgt gaaaaatgga tggtgcaatt
    14881 tgcaccatcc atttttgtta cttcgcggcg actttttctc gcagtgcgcg tttttcctct
    14941 gcgctgagga aagccatttc cagaccatta atctgtgcct ggcggatttg ctcacgggat
    15001 aacccagcag ctggcgcggc aacggtatat tcgtgaatga tatccactcc ctgtacgccg
    15061 ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa ggaacgtttt cagcggatgt
    15121 gcagccagct ctgctacggt gctggtctga atattggagg tcagacagga ttcaatacca
    15181 atttgttgct cggcgagaaa atccatcagc gcccgatctt caatggcttt tacgccatgt
    15241 ccaatacgct ccgcacccag ttcacgaatc gcctgccaga tgctttccgg cccggcagct
    15301 tcgcctgcat ggacggtaat atgccagccc gcatcacgcg cgcggttgaa gtgagaaagg
    15361 aacagacttc ccgggaatcc aagttcatca ccagctaaat caagtgcggt aatctggtca
    15421 cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg cttcgccgaa ggttcggctc
    15481 ataatgccga taagcttcgc ctgcacacca aaggtgcggc aaccttcacg tacgccatcg
    15541 atcaccgctt cgacaacacc cgctacaggc agcttatgtg ccattgccat gtagcctggt
    15601 gaaaaacgca gctcgacata gcgcaggccg ttacgggctg catcttcaat gttttcaaat
    15661 gccacgcggc ggcaggcatc aagagaggcg agaactttaa cgccccagtc aagtttggtc
    15721 agaaagctca ccagatcggg ttcgttggca atgacctgaa cgtggggaat cagtgtttca
    15781 agggattgtg caggaagcga gatattatac tggcggccaa gttcaagaat ggtctgggga
    15841 cgaatgttgc catcaaggtg gcgatggata tcagttaatg gcagggtggt atcaatcatg
    15901 gtcgcactct ttttctggat ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa
    15961 atccacgatt gcgcaacgat tttttacgtt gcgcaattgg ggttagcgaa cagcgcggat
    16021 ggcgtttatt aatccagcca cacctttttc cagtttcgaa cgtgggcagc cggcattgag
    16081 acggacaaaa ccacgacctt cttcaccata ggtatacccc ggcatgatcg cgactttttc
    16141 ttgctcgata agcgcttttt gcaacgcgtt gtcgtcaata ttcaacggac gtagatcaag
    16201 ccaggccaga taagtggatt gcgggatctg ccagttgagt tcaggaaacg cggcgttcat
    16261 tttatctgag atatacgtca ggttatcttt cagatagacg cgtaaggcat ccagccacgg
    16321 tgcaccttgc tgataggcgg cgatatgggc agttaacgcc agtaccgaag gggaagaaag
    16381 cccatcacgt cctttcagtg ccgataaata ggcatcacgg ctgctgctat tttctataat
    16441 cccgtaagca ccggtcagcg cgggaatatt gaaacttttc gatcccgatg tcagcaacgc
    16501 ccagtctccc cgcgccacat tactccaggg aatatgcggc tgctcgcccc agaccatatc
    16561 catatggatt tcatcggaaa taacccgcac atcatgacgc tcgcacaggt cagccatgat
    16621 ctccagctca tcgcacgtcc acactttccc ggtaggattc tgtgggttac acaggagcat
    16681 aattttacat tctggtttcg ccaaaacggc ttccagcttg cccatatcgc aaaaccagcc
    16741 gtcagcctgc ttctctaaag caacgggcat tactgtgcgc tggttacctt caatggcctt
    16801 gtaaaatgcg tcataggcgg gcgtgtggat caccacgcct tcacctgttt cagaccactg
    16861 acgaatcagt tctgaaacca tatagatgac agaagggcca tacaccaccg tctgcgtatc
    16921 aattgcggtg taatgctggg tggaaaacca gtgggcaata gccgcgagaa actcatcgtt
    16981 tttccagcgg ctgtagccaa atacgccgtg catcaggcgc tgattcagcg cctcgataat
    17041 gcagggggca gtggcaaaat ccatgtctga aatcgtgaac ggtaacaggt cagcagtgcc
    17101 gaaacggtca gcgacataat cccactgtgt acaccatgtg ccatgacgat ccacgacctt
    17161 tgaaaaatcg aacatatctt atccttatgc ctggacagta tgcatcagac cggccatttc
    17221 atcttttact gactgaactt gtggcccgat aacaacctgc aggttatgtt gattaagttg
    17281 tactacgcca attgcccgat tgtccttcag tgcctgcaca ttaacaagcg acatatcttc
    17341 cacagacaaa cgcagacggg taatgcagtt atcgaggctg acaatattgt cggcaccgcc
    17401 taatgcttcg aggattgcag gaacgttgta acctgattta cccggcgcac cggcaacggc
    17461 tttttcgatt gagctggcaa cttcgctatc gcgccccggg gttttcagat tgaagcgggt
    17521 gatagcgaaa cggaagatga cgtagtaaac gacaaaccag attgccgcca ccactggcac
    17581 catgtaccac ttggttgaca gaccatgcaa aataccgaac accacgaagt cgatgatatt
    17641 gccgtcggta ttaccgatgg tgacgccgag cacagacatg acggtgaagc cgaggccggt
    17701 taacagcgcg tggatgacat acagaactgg cgctacgaac aggaacagga attccagcgg
    17761 ttcggtagtg ccgccaacga cgcaggcgat caggccagaa atcagcagac ctttaatttt
    17821 atggcgattt tccgggcgcg cgcagtgata catagctaac gctgcacctg gtagaccgcc
    17881 gagaaacgca ggcattttac cttgcgaaag gaaacgcgtg acgctttcag aaaaaccgtg
    17941 agtggtcggg caactcaatt gcgcctggaa gatggtcagt gcgccgctga cggtttgacc
    18001 gcagacctcc tgcgtgccgc ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg
    18061 cagaccaaac ggcaacagca gacgttcacc ggtaccaaac agcatcggtc cgaaatcacc
    18121 cgcgctgttt atcatatggc ccaagccgct aatacccatg gcgaaaatcg gccagactaa
    18181 tggaatcacc aggccgacaa ggcccatcac cagcgaggag ataattggta cgaagcgcgt
    18241 accgccgaag aatgccagcg catccggcag gcggatatta tggaaacgct catgcagcat
    18301 ccagacgata ataccggcga tcaccgcacc gaggatcccg gtatcgatcg actggatccc
    18361 aagaatgctc tggatgttat tggctttcag aacggcggca tctgtggttg gcagaatgcc
    18421 tttattggtc aaccagaagt ttaccgcgag gttcattacc gcataaccga cgaagccagc
    18481 gaatgccgct acgcctttat tttcgcgtgc caggcccagc gggatggcga tacagaacat
    18541 gacaggcagg aaactaaaag caaacgagcc aatcttactc atccaggtaa agatagcctg
    18601 caacacgggg ttgcccagga ccgggatcag ggttatgaca tcatggctgc taagagaact
    18661 accaatgccg agcataatgc cgcagaacga caataatgcc acgggtaaca tgaaggtttt
    18721 gcctaactgc tggaagaact cccacagcgt gactttcggt gctgttttcg ccgtcataaa
    18781 acgactcctc gtagagaaca atctggattc agtaaattgc gcgagaagat aaaacgtttt
    18841 atcaaatttt agtgagtcat aaatcacatt acgcaacgat aatagcgggt ataagataaa
    18901 taaaaggtaa aacgttttat ctgtcacata atcagggagt aggtcatctg catggctacc
    18961 gccaaaaaaa taaccattca tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt
    19021 tcgctggtgc ttaatggcaa agggcgaatc tctaccgcca caggagaacg cgttaacgcc
    19081 gccattgaag agctgggatt tgtgcgcaat cgccaggcgt cggcgctgcg cggcgggcaa
    19141 agcggcgtca ttggtttgat cgtccgtgat ttatctgcgc cgttttacgc cgaattgacg
    19201 gccggattga cggaagctct ggaagcacag ggacggatgg tttttttgct tcacggcggt
    19261 aaagacggcg agcagctggc acagcggttt tcactgttac tgaatcaggg tgtcgatggt
    19321 gtggtaattg ccggggctgc aggaagcagc gatgacctgc ggcggatggc agaagaaaaa
    19381 gctatcccgg tgattttcgc ttcccgtgcc agttatcttg atgatgttga tacggttcgc
    19441 ccggacaaca tgcaggctgc acagttgttg acggagcatc tcattcgcaa tgggcatcag
    19501 cggatcgcct ggctgggagg gcaaagttcc tcattaaccc gtgcagaacg ggtggggggc
    19561 tattgtgcaa ctctactaaa atttggcctg ccgtttcaca gcgattgggt gttggagtgc
    19621 acttccagcc agaagcaagc cgcggaagct atcacggcgc ttttacgtca taacccgacc
    19681 atcagtgccg tggtttgcta taacgaaact attgcgatgg gggcatggtt tggtttgctg
    19741 aaagcagggc ggcaaagcgg ggaaagcgga gtcgatcgtt actttgagca acaggtttcg
    19801 ctggcggcat ttaccgatgc gacaccaacc acacttgatg atatacctgt tacctgggcc
    19861 agcacgccag cgcgggaact tggtatcaca cttgcggatc gcatgatgca aaaaatcacc
    19921 catgaagaga cgcattcacg caatcttatt attcccgccc ggctcattgc ggcgaaataa
    19981 tcctctcttt atctgctata cttggtagtg tcctttcctc aaggttaatg ataaaaaatg
    20041 tggcacacag gcggtgcctt ttatttttac tgaagttcac aggaggttta tgtgtttaat
    20101 tctgacaacc tgagactcga cggaaaatgc gccatcatca caggtgcggg tgcaggtatt
    20161 ggtaaagaaa tcgccattac attcgcgaca gctggcgcat ctgtggtggt cagtgatatt
    20221 aacgccgacg cagctaacca tgttgtagac gaaattcaac aactggatgg tcaggcattt
    20281 gcctggcgtt gtgatattac ttccgaacag gaactctctg cactggcaga ctttgccgtc
    20341 agtaatctgg gtaaagttga tatcctggtt aacaacgccg gtggcggtgg tcctaaaccg
    20401 tttgatatgc caatggcaga ttttcgccgt gcttatgaac tgaatgtgtt ttcttttttc
    20461 catctgtcac aacttgttgc gccagaaatg gaaaaaaatg gaggtggcgt tattctgacc
    20521 atcacttcta tggcggcaga aaataaaaat ataaacatga cttcctatgc atcatctaaa
    20581 gctgcggcca gtcatctggt cagaaatatg gcatttgacc tgggtgaaaa aaatattcgg
    20641 gtgaatagca ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca
//
LOCUS       DADLEE010000072        20691 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000072 DADLEE010000000
VERSION     DADLEE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.072"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            81..350
                     /locus_tag="JQH51_002773"
     CDS             81..350
                     /locus_tag="JQH51_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY6077062.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="JQH51_002774"
     CDS             344..709
                     /locus_tag="JQH51_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY6077063.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQH51_002775"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQH51_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY6077064.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="JQH51_002776"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="JQH51_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY6077065.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="JQH51_002777"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="JQH51_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY6077066.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="JQH51_002778"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="JQH51_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY6077067.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQH51_002779"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQH51_002779"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY6077068.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQH51_002780"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQH51_002780"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY6077069.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="JQH51_002781"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="JQH51_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="JQH51_002782"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="JQH51_002782"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY6077070.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /locus_tag="JQH51_002783"
     CDS             complement(13442..14668)
                     /locus_tag="JQH51_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY6077071.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /locus_tag="JQH51_002784"
     CDS             14849..16447
                     /locus_tag="JQH51_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY6077072.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="JQH51_002785"
     CDS             complement(16429..17187)
                     /locus_tag="JQH51_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY6077073.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="JQH51_002786"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="JQH51_002786"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY6077074.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="JQH51_002787"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="JQH51_002787"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY6077075.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="JQH51_002788"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="JQH51_002788"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY6077076.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="JQH51_002789"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="JQH51_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5692 c         5217 g         4893 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gctaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DADLEE010000073        20521 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000073 DADLEE010000000
VERSION     DADLEE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.073"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JQH51_002790"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JQH51_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY6077077.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JQH51_002791"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JQH51_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY6077078.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JQH51_002792"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JQH51_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY6077079.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQH51_002793"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQH51_002793"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY6077080.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQH51_002794"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQH51_002794"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY6077081.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JQH51_002795"
     CDS             4449..4814
                     /locus_tag="JQH51_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY6077082.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JQH51_002796"
     CDS             4964..5482
                     /locus_tag="JQH51_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAY6077083.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JQH51_002797"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JQH51_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JQH51_002798"
     CDS             6713..7195
                     /locus_tag="JQH51_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY6077084.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQH51_002799"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQH51_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY6077085.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQH51_002800"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQH51_002800"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY6077086.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQH51_002801"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQH51_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY6077087.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQH51_002802"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQH51_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY6077088.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQH51_002803"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQH51_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY6077089.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JQH51_002804"
     CDS             10864..11730
                     /locus_tag="JQH51_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY6077090.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLMHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JQH51_002805"
     CDS             11751..13037
                     /locus_tag="JQH51_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY6077091.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQH51_002806"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQH51_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY6077092.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JQH51_002807"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JQH51_002807"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY6077093.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JQH51_002808"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JQH51_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY6077094.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JQH51_002809"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JQH51_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY6077095.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JQH51_002810"
     CDS             complement(16985..17275)
                     /locus_tag="JQH51_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY6077096.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQH51_002811"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQH51_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY6077097.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JQH51_002812"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JQH51_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY6077098.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JQH51_002813"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JQH51_002813"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JQH51_002814"
     CDS             19137..20378
                     /locus_tag="JQH51_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6077099.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5356 c         4814 g         5051 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac ataggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atgtttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attgcaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccaa gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgttgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtatttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLEE010000074        20288 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000074 DADLEE010000000
VERSION     DADLEE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.074"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="JQH51_002815"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="JQH51_002815"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY6077100.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JQH51_002816"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="JQH51_002816"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY6077101.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..2943)
                     /locus_tag="JQH51_002817"
                     /pseudo
     CDS             complement(1728..2943)
                     /locus_tag="JQH51_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="JQH51_002818"
     CDS             complement(3183..4388)
                     /locus_tag="JQH51_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077102.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="JQH51_002819"
     CDS             complement(4488..4844)
                     /locus_tag="JQH51_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY6077103.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="JQH51_002820"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JQH51_002820"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="JQH51_002821"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="JQH51_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY6077104.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="JQH51_002822"
     CDS             6774..9830
                     /locus_tag="JQH51_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY6077105.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="JQH51_002823"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="JQH51_002823"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY6077106.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="JQH51_002824"
     CDS             10337..10525
                     /locus_tag="JQH51_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077107.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="JQH51_002825"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="JQH51_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY6077108.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="JQH51_002826"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="JQH51_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY6077109.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="JQH51_002827"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="JQH51_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY6077110.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="JQH51_002828"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="JQH51_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY6077111.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="JQH51_002829"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="JQH51_002829"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY6077112.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="JQH51_002830"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="JQH51_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY6077113.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="JQH51_002831"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="JQH51_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY6077114.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /locus_tag="JQH51_002832"
     CDS             complement(17801..18037)
                     /locus_tag="JQH51_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY6077115.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="JQH51_002833"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="JQH51_002833"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY6077116.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5316 a         4862 c         5177 g         4933 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc atggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattgata
    20281 tatgctga
//
LOCUS       DADLEE010000075        20084 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000075 DADLEE010000000
VERSION     DADLEE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.075"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="JQH51_002834"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="JQH51_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY6077117.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="JQH51_002835"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="JQH51_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY6077118.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="JQH51_002836"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="JQH51_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="JQH51_002837"
     CDS             1631..2341
                     /locus_tag="JQH51_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY6077119.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="JQH51_002838"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="JQH51_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY6077120.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="JQH51_002839"
     CDS             complement(3469..4119)
                     /locus_tag="JQH51_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY6077121.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6480
                     /locus_tag="JQH51_002840"
     CDS             4282..6480
                     /locus_tag="JQH51_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY6077122.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="JQH51_002841"
     CDS             complement(6449..6889)
                     /locus_tag="JQH51_002841"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY6077123.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="JQH51_002842"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="JQH51_002842"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY6077124.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="JQH51_002843"
     CDS             7607..8305
                     /locus_tag="JQH51_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6077125.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="JQH51_002844"
     CDS             8534..9742
                     /locus_tag="JQH51_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077126.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="JQH51_002845"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="JQH51_002845"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY6077127.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="JQH51_002846"
     tRNA            11849..11925
                     /locus_tag="JQH51_002846"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="JQH51_002847"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="JQH51_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY6077128.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="JQH51_002848"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="JQH51_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY6077129.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="JQH51_002849"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="JQH51_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY6077130.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="JQH51_002850"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="JQH51_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY6077131.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="JQH51_002851"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="JQH51_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY6077132.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="JQH51_002852"
     CDS             complement(18540..19811)
                     /locus_tag="JQH51_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6077133.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4813 a         4973 c         5275 g         5023 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatagct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agca
//
LOCUS       DADLEE010000076        19725 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000076 DADLEE010000000
VERSION     DADLEE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19725
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.076"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            247..1344
                     /gene="mltA"
                     /locus_tag="JQH51_002853"
     CDS             247..1344
                     /gene="mltA"
                     /locus_tag="JQH51_002853"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY6077134.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1582..2388
                     /gene="tcdA"
                     /locus_tag="JQH51_002854"
     CDS             1582..2388
                     /gene="tcdA"
                     /locus_tag="JQH51_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY6077135.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2439..2882)
                     /gene="csdE"
                     /locus_tag="JQH51_002855"
     CDS             complement(2439..2882)
                     /gene="csdE"
                     /locus_tag="JQH51_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY6077136.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2882..4087)
                     /gene="csdA"
                     /locus_tag="JQH51_002856"
     CDS             complement(2882..4087)
                     /gene="csdA"
                     /locus_tag="JQH51_002856"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY6077137.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4279..4506
                     /locus_tag="JQH51_002857"
     CDS             4279..4506
                     /locus_tag="JQH51_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY6077138.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4857..5774
                     /locus_tag="JQH51_002858"
     CDS             4857..5774
                     /locus_tag="JQH51_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY6077139.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5793..6188
                     /locus_tag="JQH51_002859"
     CDS             5793..6188
                     /locus_tag="JQH51_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY6077140.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6181..7281
                     /gene="rlmM"
                     /locus_tag="JQH51_002860"
     CDS             6181..7281
                     /gene="rlmM"
                     /locus_tag="JQH51_002860"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY6077141.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7325..8056)
                     /gene="fucR"
                     /locus_tag="JQH51_002861"
     CDS             complement(7325..8056)
                     /gene="fucR"
                     /locus_tag="JQH51_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY6077142.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8114..8536)
                     /gene="fucU"
                     /locus_tag="JQH51_002862"
     CDS             complement(8114..8536)
                     /gene="fucU"
                     /locus_tag="JQH51_002862"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY6077143.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8538..9957)
                     /gene="fucK"
                     /locus_tag="JQH51_002863"
                     /pseudo
     CDS             complement(8538..9957)
                     /gene="fucK"
                     /locus_tag="JQH51_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10066..11841)
                     /gene="fucI"
                     /locus_tag="JQH51_002864"
     CDS             complement(10066..11841)
                     /gene="fucI"
                     /locus_tag="JQH51_002864"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY6077144.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11874..13190)
                     /gene="fucP"
                     /locus_tag="JQH51_002865"
     CDS             complement(11874..13190)
                     /gene="fucP"
                     /locus_tag="JQH51_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY6077145.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13737..14384
                     /gene="fucA"
                     /locus_tag="JQH51_002866"
     CDS             13737..14384
                     /gene="fucA"
                     /locus_tag="JQH51_002866"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY6077146.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14412..15560
                     /gene="fucO"
                     /locus_tag="JQH51_002867"
     CDS             14412..15560
                     /gene="fucO"
                     /locus_tag="JQH51_002867"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY6077147.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15613..16458)
                     /gene="xni"
                     /locus_tag="JQH51_002868"
     CDS             complement(15613..16458)
                     /gene="xni"
                     /locus_tag="JQH51_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY6077148.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16479..17846)
                     /gene="sdaB"
                     /locus_tag="JQH51_002869"
     CDS             complement(16479..17846)
                     /gene="sdaB"
                     /locus_tag="JQH51_002869"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY6077149.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17904..19193)
                     /locus_tag="JQH51_002870"
     CDS             complement(17904..19193)
                     /locus_tag="JQH51_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY6077150.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4868 a         4834 c         5204 g         4819 t
ORIGIN      
        1 tatgagcccg acgagctacc aggctgctcc accccgcgtc cgtggatgcg cactatactc
       61 cgctcgcgtt ttcatgcaac ctttttttta ccccgatcac ggatttagca tgattttgaa
      121 caaaaacaaa ccactaaagc ctattttttg tgcaaaattt gcgtcaggga gaggctgctc
      181 atttcattaa acgcggcggt ttgttatctt cgttgcgcct tattttttaa cctgaagaag
      241 agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt
      301 gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg gaaatttacc
      361 cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa cgccggtgat
      421 tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt
      481 aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg caatatgcgc
      541 cagttcggca ttgatgcctg gcagatggaa ggtgccgaca actatggtaa cgtgcagttt
      601 acaggctatt acacgccggt aattcaggcg cgccataccc gccagggcga gttccagtat
      661 cctatttacc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga gatctacgcc
      721 ggggcattgg gcgataaata catcctcgct tacagcaact ctctgatgga taacttcatt
      781 atggatgtgc agggtagtgg gtatatcgac tttggtgatg gcagtccgct taactttttc
      841 agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgct gatcgaccgt
      901 ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcattgggg cgaaacacac
      961 agtgaagccg aggttcgcga gctgctggaa cagaacccgt ctttcgtctt ctttaaaccg
     1021 caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg tgcttctgtt
     1081 gcctccgatc gttcaattat tccgccaggt actaccttgc tggcagaagt gccgctgctg
     1141 gataataacg gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc gttggatgtc
     1201 gggggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc ggaagccgga
     1261 caccgcgcag gttggtacaa ccactatgga cgggtttggg tgctgaaaac ggctccgggc
     1321 gcaggtaacg tctttagcgg ctgatgtgat attctgtatg ccgtttttac ggatatgaca
     1381 ggggaggggt aacctcacat cctgattatt gactaagtgc gcgttgttca taccggatgc
     1441 ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc aaaatattgc aggagttatg
     1501 taggcctgat aagcgtagcg catcaggcat ttatgcgttt agggtgaggg ataacctcgc
     1561 cctttttaat tctgaggtgc tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt
     1621 ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac tgtttgctga cgcgcatatt
     1681 tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg aagcgctggc gcgcacgggg
     1741 attggcgcaa tcacgcttat cgatatggat gatgtgtgcg tcaccaatac caatcggcaa
     1801 attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg aagtcatggc ggagcgcatt
     1861 cgccagatta acccggagtg ccgtgtaacg gtggtggatg atttcgtgac gccggataac
     1921 gtagcgcaat atatgagtgc cggttattcg tacgtgattg atgccattga tagtgtacgg
     1981 cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt
     2041 ggcgcgggtg ggcagattga cccgacgcag attcaggtta ccgatctggc gaaaacgatt
     2101 caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa
     2161 aacagtaaag gtaagctcgg cgtggattgc gtgttttcta ctgaagcgct ggtgtacccg
     2221 cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag aagggccgaa gcggatggat
     2281 tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca cctttgggtt tgttgcggtt
     2341 tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc agggttaaac aatcaggtcg
     2401 gatgagacgc gaacgtctta tccgacccgg ttctgtgctt aaacctgctt cgtcgcagcg
     2461 ataatcgcct cgcttaacgc atttaacccc tggctgcgtg aggcgctaag ctgcgcacgt
     2521 aatcccagct catcaaacaa tgccagcggc gactgtgcct gtaattcggc ggcggttttc
     2581 ccctcaacgg cagtcaacaa caccgccaac aggccgcgca caatgcgccc ttcgctgttg
     2641 ccaaagaagt gcattttgcc gttttcagcc acggtatatc ccagccagac gcggttttcg
     2701 catccggcaa tctctttagc ctgcgctttt aactcgtctg gcaaagccgg aagctgtttc
     2761 cccagcatga tcaactggcg atatttatct tcccattgcg acaacggtgc gaaggtattg
     2821 cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc
     2881 attaatccac caataattcc agcgcgcggt caacggcatt caccagcgca tccacatcac
     2941 tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc ggttacgcct aattctgcca
     3001 gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat accgtactcc gccagtagcg
     3061 tcaccatatc gctgtgatga acgccagcaa aatcaaaggc cagcaggctg gaatcctggc
     3121 agcggaatga acgaaagcct ggacgtttcg ccagcgcttc ttctgcaagc gttgctaagc
     3181 tacggctcca gctttcggcc tggttgatat cgtaatctgc cagccattcc agcgccgcgc
     3241 ttaatcctat gacgccagcg acatttggcg ttcctgcttc cagtttccac ggcgcagatt
     3301 gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt gccgccaccc agccagggcg
     3361 acatcgcttc cagcagttct gatttgccat acagcacgcc gatacccgtc gggccataca
     3421 gtttgtgacc agagaaagca tagaaatcga tatccagttg ctgaacatcc gcggggaaat
     3481 gcaccgtccc ctgagcacca tcaaccatca ccaccatccc ggctgaatga gcaaaggtaa
     3541 tcgctcgcgc cagatccggg caaccgccag tcacgttcga catctgaccc aacgccagaa
     3601 tccgactacg gggagtaatc agctctggca acaaatcgac atccggcagt cgctgcgcat
     3661 taagcggcaa tttcaccact ttggctctag tttgttgggc gaccatcagc caggggacga
     3721 ggttagcgtg gtgttctgcc acgctgacaa taatctcatc gcccggttgc agacgcggac
     3781 gcgcatagca ttgtgccacc atgttgatgg attcagtggt gccgcgcgtc cagacgatag
     3841 ttttatcatc cggtgcattc agtaattgcg ccactttctc tcgtgcagct tcataacgcg
     3901 cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac gtttccggcg ctcaggctgt
     3961 aaaactgtcg agtggcttca accacggctt caggtttaag cgcggtcgcg gcgctgtcga
     4021 gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg aaactgcgcg ggattaaaaa
     4081 cgttcatggt actcctcggc ttgatgcacc gatcgtgacg caaaattcac tttgatacaa
     4141 ggagagtgct aaccatcaga attatctgga tatcctggcg aagccgcgta agtaacatat
     4201 gctgaatagt gttaggcgtt aattttagcc tgttaatcaa tactattaag atttattaat
     4261 cgttttaagg agaagatgat gaaaaagact gccgcaatta tttctgcctg tatgctgact
     4321 tttgccctga gcgcctgttc cggttcgaac tatgtgatgc acaccaatga cggacgtacc
     4381 atcgtctctg acggcaaacc acagactgat aacgatatcg gtatgatttc gtataaagac
     4441 gctaatggca acaaacagca gatcaaccgt actgacgtga aagagatggt cgaactggaa
     4501 cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc
     4561 actatggaca gacagggtaa atgtacagga agtgaaaaaa ggtagctttg ctaccatggt
     4621 ctgaatcgca gaccaattgc aaacacaaca acacaacatc acaaccgtaa gccaaaagtt
     4681 caccagaata cgcattccga taaaactttt cgttccggct caggaagtgc cgccactata
     4741 ggtatttgct ggtagaagct caacggacaa tttataatgg ctcagattaa aaaaactaat
     4801 aggttacaca gtgtgatcta attgttaaat tcatttaaca tcaaagttta aaagccatgt
     4861 ctaaacgatt accaccgcta aatgccttac gagtttttga tgccgcagca cgccatttaa
     4921 gttttactcg cgcagcagaa gagctttttg tgacccaggc cgcagtaagt catcaaatca
     4981 agtctcttga ggattttttg gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc
     5041 tgaccgagga agggcaaagc tatttcctcg atatcaaaga gatattttcg caattaaccg
     5101 aagcgacgcg taaactccag gcccgtagcg ccaagggggc gttgacggtc agtttactcc
     5161 ccagtttcgc cattcattgg ttggttccgc gactttccag ctttaattca gcttatccgg
     5221 gaattgacgt tcgaatccag gcggttgatc gtcaggaaga taagctggcg gatgatgttg
     5281 atgtggcgat attttatggt cggggcaact ggccggggct acgggtggaa aaactgtacg
     5341 ccgaatattt attgccggtg tgttcgccgc tactgctgac aggcgaaaaa cccttgaaga
     5401 ccccggaaga tctggctaaa catacgttat tacatgatgc gtcacgccgt gactggcaga
     5461 catatacccg acagttgggg tttaatcata tcaacgttca gcaagggcca atttttagtc
     5521 atagcgccat ggtgctgcaa gcggctattc acgggcaggg agtggcgctg gcaaataacg
     5581 tgatggcgca atctgaaatc gaggccggac gtcttgtttg cccgtttaat gatgttctgg
     5641 tcagtaaaaa tgctttttat ctggtttgtc atgacagcca ggcagaactg ggtaaaatag
     5701 ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc
     5761 gttatgaaca ataatttacg tagggtacga ccatgaccag ccgttttatg ctgattttcg
     5821 ccgccattag cggcttcatt tttgtggcac tgggcgcttt tggtgcgcat gtgttaagta
     5881 aaaccatggg ggccgttgag atgggctgga tccaaaccgg tctcgaatac caggcgtttc
     5941 atacgctggc gatcttaggt ctggcggtgg caatgcagcg tcgcatcagt atctggtttt
     6001 actggagtag cgttttcctc gcgttaggca cggtgctgtt cagcggcagc ctttattgcc
     6061 tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc ggttggcggc gtgagcttcc
     6121 ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc
     6181 atgaataagg ttgtattgct gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt
     6241 accgataaag ccggccagcg ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt
     6301 tatgtcattt atgaatgtta tcaacctgat gatggcgata agttaatccg tgagctgccg
     6361 ttcagttcat taatttttgc ccgccagtgg tttgtggtgg gggaactcct gcagcatttg
     6421 ccgccagaag atcgtattac ccccattgtc ggcatgttac agggtgtagt agagaagggc
     6481 ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca aagagttgct gaaattctgc
     6541 cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg ctggggtgct ggcgaactat
     6601 gaaacgccga agcgtccggt tgtgcatgta ttctttattg caccaggatg ctgctatacc
     6661 ggttactcat acagcaacaa taattcgccg ttctatatgg gcattccgcg cctgaaattt
     6721 ccggcagagg cgccgagtcg ttccacgctc aaactggaag aggcatttca tgtgtttatt
     6781 cctgcggatg agtgggatga acgcctggcg aacgggatgt gggcggtgga tttaggcgct
     6841 tgccctggcg gctggaccta ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac
     6901 aacggcccga tggcgcaaag tctgatggat accgggcagg tgacgtggct gcgggaagac
     6961 ggtttcaaat tccgtccgac acgcagcaat atctcctgga tggtatgcga tatggttgaa
     7021 aaaccggcta aagttgcggc attgatggcg cagtggctgg ttaatggctg gtgccgtgaa
     7081 accattttca atctcaaact gccgatgaaa aaacgctacg aagaagtatc acacaatctg
     7141 gcgtatattc aggcacagct tgatgaacat ggcataaatg ctcagattca ggcgcggcag
     7201 ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc gcatctgggc tgcggtgggt
     7261 ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc gtctccggcc tgctaccctt
     7321 tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc atcagaaata atgtgcgtta
     7381 cctcatccag atgcccgatg cgggcttccc ctgaacgatt aaatttactt ttatcaatca
     7441 ataacaacga ttgcgcggca cgttttaata gcatcgattt gtaatcagcg ttgatcgcat
     7501 tggagtccca cagtgcgccg ctgctatcga tcccttcaca agaaaaaata aacagatcga
     7561 tttccagcga tttaagttgg gaaatcagcg agggattgac gtaacagcca tatttgcgct
     7621 caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg tttaccgagt tcatggcaaa
     7681 tcggatggct attggtgaag acctgaatgt tgatgtcagg caactggcgt gccagatacc
     7741 agcaagttga actggcgtct aaggctatca ccatcccttc ttcaatccac gcgagcgcct
     7801 cgcgcgcgat atctgcttta tgcgcataat ggcttttcag cctgatgtga aagggatcgc
     7861 cactgtcttg attttgacgg tggatatatt tagcgcgtcc atgattgcgc agaattttac
     7921 cctgcgtctg taattcattg agatcgcgac gaatggtttc tttactgacc tttagctgtt
     7981 cagagagagc ttccgtggtc aggctggtat ggttcagcag caggtcgact atcgcttgct
     8041 ggcgtgccgc tttcatattt ttcaccccat cattcgataa ccgcccgcgt catgcgggcg
     8101 taccggtatg agattacggt gttacccctt tttttaaaag aatattcccg tacttcgctc
     8161 gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg ttcataaaac gcaaaacgat
     8221 tgatgcggat gatgtcagga cacggggctt gtagtgaaag cgcattacgg taacgtcgtt
     8281 ctacttcagg atcgagagtg tcaccctcta ccgccgccat catcaccagc ggcggggcat
     8341 aactgtccag ctcaaataac gggataatcg cctggagcaa gtcgctcacc aacaggccat
     8401 cagcgcggat cacctgcggt tccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa
     8461 tttcatctcc atgtcccatc tctgccagca ctttcaatag ttcgggagaa attaacggcg
     8521 aaattgtttt cagcatttca cacttcctct ataaattcag gttcagtttg cgggtagaaa
     8581 taacggtact gataatgaat ctgtgcacgg gcttcttccg ggctgttaaa ttcccctacg
     8641 ccataccaac cgaacagcgc agctcctgcg acggtcgttt cggcatcgtc gagaactttt
     8701 accggaatat caagcatatt ggctttaatc tgattccaca atgtgttgcg acttcctcca
     8761 ccgactaaca ataattcata ggctttaaag tgcccgattt tttccagcat ctgtagattg
     8821 cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat agaaatgccc ccgcgtggta
     8881 ttaagcgtca ctccttgcca gccagcgttc tgacacgaca ataaatcaca ctgcattttc
     8941 acaccatccg cgccaggtgc gatcagacga gcttcttcaa tcaacatttg ccagggtgtt
     9001 tcagccgtcc agaacagttt tctcacccat tccagcacgc cggatgccag ccattgcata
     9061 cctgggttat acaaccatgc ctggctatct agttcgcagg tggaaccggc gtactgactt
     9121 aacagcgaag tatcaaccta ggcgctgcga accattaaaa tttcccatgt accggaggag
     9181 agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct
     9241 gcggaaatca ccggtatgcc aacgggtaag ccgagcattg ctgcggcgtt gttctgtagc
     9301 gtaccaatct gttcacccgc ttccaccaga cgagggaaga gtcggcgtgg aataccggtg
     9361 gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat ccagcatctg gctggttccg
     9421 gccatcgtga tatcagtagt gaattcgctg gttaaacggt ggttaatcag cgacgaaatg
     9481 aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat tttctttcaa ccacaccaac
     9541 ttatataacg tattgaaact aaaggctccg acgccagaaa tagcctgcaa ccgctgtgcg
     9601 gagattaacc gttcaatatt gtccataacc gctgctgttc gcggacattt ccagctaatg
     9661 atcggataga gcagattgcc ttgcttatct accagagcgc catccacact aaaggtggtg
     9721 acggcgatac cgcggatgtg gcattcagtc agttcactat tgatttgccg acagcaatca
     9781 gcaaagcgtt gcaaaatggc agtctaaaga ccactggtgc caggtgttgt tttccatcgc
     9841 gatatcgctg gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc
     9901 gatggccctg acattggtcg cgccacagtc gagtaccagg ataacttctt gtttcataat
     9961 cgctcctgca atatagccgg ataacattgt ttatccggct aaccactctt gcttgttcag
    10021 gcccggtagc cggagcgacc gggcatcaca tcagggtgta atgtattaac gcttgtacaa
    10081 cggaccgtag ttctggcaag cgcggtaatc ctggccttca atatccatgc cgtgcgcagc
    10141 ccaggcagaa ggacgataca ctttggtctc ttcaacgttg tgcatacata ccgggatacg
    10201 cagcatggag gcaagggtga taaagtctgc gccaacgtgg ccgatggtca gaactccgtg
    10261 gttggcaccc cagttcgcca ttaccgaata cacatccgta aacggccctt taccagtgag
    10321 gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg cgtttgttga ggatgtcatg
    10381 cacatccttc ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg gtcccaggcc
    10441 tttgatgatg ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga
    10501 agagtaaccg ccgccacgga agtattcgtg gatcgccggg caccattcgg tagcggcgag
    10561 gcaagcgtca gcctcttgct gagagatttc ccagtgcggc ttcatcgtcg ggttaccttc
    10621 gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa cctgagttga tcaaatggat
    10681 gatgccgtgt tctgccagtc cgtccagttt atgccccgtt acacgctcaa ttgcttctgg
    10741 tgaccagtag gtacgcacat cggcaaatac ctgagcggtg ccggtgagct ggtgacccat
    10801 tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac
    10861 gccattccag tcaaatgaac tgttgaggat cgcttcggcg gtgtcaccat tgggatattg
    10921 atcggtccag tgacgttgcc cctggaagcc cgcagcgatg gcgttgtagc caagtgattc
    10981 ttccacgcga ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc ggatacacat
    11041 cgccatcagt aaactttcgc gcagaactgc gcggctttgc tcggcattgc gttgatactg
    11101 tttgttattt tcatcttcgc catagcggaa gtttttatca gcccaggcca gtgccatttc
    11161 cagttcggct tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg tcatatccac
    11221 cgcctggact ttcattccca gccaggattc aaagaagttg tgatcaacaa tagaaccggc
    11281 gatacccatc gaaacgccgc ccagcgacag atagctttta cctttcatgc tggcgacggc
    11341 caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc
    11401 atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg
    11461 agctgccaga gccgcagcca ggtaaacagc gccggggcgt tcagtgccgt taaagcccca
    11521 aatggctttc gggcgggttg gatccatgtc gatggtttca ctgccatagc accagcaagg
    11581 cgttaccgta atggtgaggc ctacattctg gctgctgaat ttttcttcgc aagcagcggc
    11641 ttcagccata cccgcgatac aggtatcgga aatgacacac tcgacggcag ctccgcaggc
    11701 atggcgcagt ttctcggtca gcagtgcggc cgtagctttc gccatattca ttgtttgttc
    11761 ttcaagcgac tcacgaacac ccatgcgacg accgtcaata accgggcgga taccaatttt
    11821 cggtaagctg atttttttca ttacagattc ctcactttat tcggaaaata tgttcagtta
    11881 gttgccgttt gagaacggaa acgggcaaag ataaagatga ccgcgaagca gagtgcgggg
    11941 atcagttcag cagtggggat gttgcccgcc gcgtcactga caaaacccat gaccggagtg
    12001 acaataccgc cgccaataat ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg
    12061 ccgagattct taatgcccag cgagaagatt gttgggtact gaatcgacat aaaggcgctg
    12121 cataaagtca gggctattaa gcccacatga ccgccagcga aggctgagat caggcacagt
    12181 gccatagcga ttaatgcgta ggcggccagg actttgtgtg gtgcgaagcg actgatgagc
    12241 caggtaccgg tgaaacgacc aataaagaag cacaccatgg ttccggttaa atagttagcg
    12301 gcaaagcctg cagtcatacc tagaatttct tctacagcgt agcgaatcaa atagctccag
    12361 caggccgttt gtgcgccgac atagcagaat tgcgctaata ccgcccagcg ccagtgacga
    12421 atacgcgcca gacgagaaag cgatgcggag aacgatcctt gtttggcgtc actgtgatta
    12481 tcactctgca atgccgggaa tttcgtcagc atgatcagca gggcgaccag taacacgata
    12541 gccacgatga tcatataagg tgtctgtacc gataatacca ggctgtgttt atacgcactc
    12601 aattgctctg gagacatttt atcgagaacg tcttgcgatt gatgtggcac gttagacaaa
    12661 ataagacttt gcccaaagac aaccgcgata attgcgccaa acgagttaaa tgtttgcgca
    12721 agatttaagc ggaagtgact actgctttcc ggccctaata ccgtaacaaa agggtttgcg
    12781 gcagtttcca gacaacctaa tccggctgca ataataaata ggccaactaa aaataaggtg
    12841 tagttcatta tttctgcagc gggccagaat aatgcagcgc ccaaggcata taaaaataat
    12901 ccggtaataa tccctgcttt ataactgagt tttttcatca atatcccagc agggattggg
    12961 ataatgaaat aaccaaagta aaaggccgat tggatcaggc cagcctggaa atttgtcagc
    13021 gtaaaagcct gctggaattg aggtaataaa atgtcgttaa ggttattggc taccgcccaa
    13081 agaaaaaaca gtgagcacag cagcgcgaat ggaataatgt aacttctgct ttgccctgca
    13141 tctttatcta ccgcacggta actctgcgtt tgtattgatg tgtttcccat agcatcctct
    13201 taggttcaga agcttaagca cgtaatcggt tatgttttca aaagtgcaaa accagcgagt
    13261 tcgggcttca tatcggtgaa tatttgcctg tctgaaatga actcatggat tcattttcaa
    13321 ataaaaaacg ctctgtaata tgacggcggt cacacttatt agctggaagg tgactttatg
    13381 tgactaccat cactttaata atcgaaattt catgcccgtt tcagaaaata aatcataaaa
    13441 aacacaaacc agatgaccgt attaattgtt tgtgccgaaa acgggcattt ctggcgaggg
    13501 gaaatggaaa aatgaccgtt ttgataaata tttttaagag cggtcatttt taacggggtt
    13561 ataaaaagag aacttgcagt tatttactgt gattagttga gctaggtcac aaaatataag
    13621 cttgtgaatt tctttcttgt taatcaggaa gaagatggtg ggtaattaaa cggctaattc
    13681 aatagtgtga aaggaacaac attattgccc tgttttgaat cagagagagg tagctaatgg
    13741 aacgaaataa acttgctcgt cagattattg acacttgcct ggaaatgacc cgcctgggac
    13801 tgaaccaggg gacagcgggg aacgtcagtg tacgttatca ggatgggatg ctgattacgc
    13861 ctacaggcat tccatatgaa aaactgacgg agtcgcatat tgtctttatt gatggcaacg
    13921 gtaaacatga ggaaggaaag ctcccctcaa gcgaatggcg tttccatatg gcagcctatc
    13981 aaagcagacc ggatgccaac gcggttgttc acaatcatgc cgttcattgc acggcagttt
    14041 ccattcttaa ccgatcgatc cccgctattc actacatgat tgcggcggct ggcggtaatt
    14101 ctattccttg cgcgccttat gcgacctttg gaacacgcga actttctgaa catgttgcgc
    14161 tggctctcaa aaatcgtaag gcaactttgt tacaacatca tgggcttatc gcttgtgagg
    14221 tgaatctgga aaaagcgtta tggctggcgc atgaagttga agtgctggcg caactttacc
    14281 tgacgaccct ggcgattacg gacccggtgc cagtgctgag cgatgaagag attgccgtag
    14341 tgctggagaa attcaaaacc tatgggttac ggattgaaga gtaatttcgt aaagcaacaa
    14401 ggagaaggat gatggctaac agaatgattc tgaacgaaac ggcatggttt ggtcggggtg
    14461 ctgttggggc tttaaccgat gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca
    14521 ccgataaaac gctggtgcaa tgcggcgtgg tggcgaaagt gaccgataag atggatgctg
    14581 cagggctggc atgggcgatt tacgacggcg tagtgcccaa cccaacaatt actgtcgtca
    14641 aagaagggct cggtgtattc cagaatagcg gcgcggatta cctgatcgct attggtggtg
    14701 gttctccaca ggatacttgt aaagcaattg gcattatcag caacaacccg gagtttgccg
    14761 atgtgcgtag cctggaaggg ctttccccga ccaataaacc cagtgtaccg attctggcaa
    14821 tccccaccac agcaggtact gcggcagaag tgaccattaa ctacgtgatc actgacgaag
    14881 aaaaacggcg caagtttgtt tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg
    14941 atgctgacat gatggatggt atgcctccgg cgctgaaagc cgcgacgggt gttgatgcgc
    15001 tcactcatgc tattgagggg tatattaccc gtggcgcgtg ggcactaacc gatgcactgc
    15061 acattaaagc gattgaaatc attgctgggg cgctgcgagg atcggttgct ggtgataagg
    15121 atgccggaga agaaatggcg ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg
    15181 ggttagggtt ggtgcatggt atggcgcatc cactgggcgc gttttataac actccacacg
    15241 gtgttgcgaa cgccatcctg ttaccgcatg tcatgcgcta taacgctgac tttaccggtg
    15301 agaagtaccg cgatatcgcg cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag
    15361 gggcgcgtaa tgccgctgtt gaagcggtgt ttgccctcaa ccgtgatgtc ggtattccgc
    15421 cacatttgcg tgatgttggt gtacgcaagg aagacatttc ggcactggct caggcggcac
    15481 tggatgatgt ttgtaccggt ggcaacccgc gtgaagcaac gcttgaggat attgtagagc
    15541 tttaccatac cgcctggtaa atgcgctgat gtgataatgc cggatacgat ttgcggcgta
    15601 tccggctcgc cgttaccgta ccaaccgcaa ttgctgtaaa ttgccgtcga tatgcaaatc
    15661 ggtttgtaag cgggcaatat cgcggcacag aaacgccatc tctttatggg tttctaattt
    15721 tttgcgccac ttttcggcaa ccgcatccag attctcatat atcccttcca gactctgaaa
    15781 ctcgaccagc agctgcgtgg cgctttttgg tccgattccc gcaacacccg gtacctttga
    15841 actgctgatc cccgcaagtc cccagtaatc gggcaactgc tgcggttgaa cgccaaattc
    15901 tttatcgata aatggcgcat ccagccaacg tttctggaag taatcacgaa tacgtaatgt
    15961 cggtgaaagt aactgacagt agcctttatc tgtcgaaaca atcgttgcct gatgcccggc
    16021 ctgtgtcact ttgaccgcca gtgtggcggc taagtcatcg gcttcgttgc cgctggctga
    16081 ccagcacggg acgccgcgtt gctcaaaggc ggcgcgtaat gcaggcatct cgtcgtgcaa
    16141 ctcttccggc attggcggtc gatccgcttt gtaatctggt aaacgctgat gacgccagcc
    16201 gctactgcgg ttttcatcat caaaaacggc gaccgcgtgg gttggctggc tgtgcataat
    16261 gagctgatcg agcgcatgct ggcaggtttc gacacagggc gaaccctgaa cggcatgaat
    16321 gcgacggata agattcagtg catcgacaat aagcaaatga acagccacga taaccccctt
    16381 aaagatcagg cgataagggt aacattcgat gccggaacag gctatggctg gttgagaaaa
    16441 caggaagagg cttcgcaaaa cgaggcctct gggagagatt aatcgcaggc aacgatcttc
    16501 atcgccaggc cgccgcgaga ggtttcgcgg tacttggcgt tcatgtcttt acctgtttcg
    16561 tacatggttt cgataacttt atcgaggcag acgcgcggtt cgctggtacg gcgcagtgcc
    16621 atacgcgcgg cgttcaccgc tttcactgct gcaatggcgt tacgctcaat gcacggtacc
    16681 tgaacctgcc ccgcgaccgg gtcacaagtc agaccgaggt tgtgctccat cgcgatttcc
    16741 gctgcgatgc aaacctgcgc cgggcttgcg cctaacagtt ctgccagacc agctgccgcc
    16801 atagagcacg ccacgccaac ttcaccctgg caacccactt cggcaccaga aatcgacgcg
    16861 ttcatcttat aaagagaacc aatggcgctg gctaccagca ggtaacgagc cagtgagtta
    16921 gcgttcactt cgcggataaa cttatcgtag tacgccagca cagccgggat aatcccgcac
    16981 gcaccgttag tcggcgcagt caccacgcga ccgccagcag cgttctcttc gttcactgcc
    17041 agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt cagtggtggt tttatcctgg
    17101 ctgaccagca tccggcgtag tgccgcagca cggcgtggaa cgcgcagttt gccaggcaac
    17161 acgccttcgg tggaaatacc gcgctcaata ccgccacgca tgacttccca gacgttcgcc
    17221 aggtgctgtt ccagctcttc tttactgtgc agcgccagct cgtttttcat catcaggcca
    17281 gagagtgaca gcccggtttc ctgacaatgt ttttgcagat cggctgctga actgtacgga
    17341 taaggaactt caaccggtgc gctatcctgc tggccaaaat gctcttcatc aacgataaag
    17401 ccaccgccaa tagagtagta agtctggctg taaacgactt tatcgcccgc cagcgcggta
    17461 atgcgcatac cgttttcatg cagagaaagg ttgtctgcat ggaagttcat gcactgatca
    17521 accgggaact ccacttcatg ctgaccgttt gccagcatca ggcgaccatg agtattcaca
    17581 tcctgaataa aaccggggat ggaatcgata tccacggtat ccggcaggtt acccgccagg
    17641 cccataataa tggcgatatc tgtgtggtgg cctttaccag tcagagagag cgagccgtac
    17701 acgtcaacca ccacgcgggt cacgtcttta agcaggttac gggcaatcag atcgtcggta
    17761 aattgtttac ccgctttcat tggtccaacg gtatgagaac tggaagggcc aatgccgatt
    17821 ttgaaaatat cgaatacgct aatcatagga aatacatcgc gttaaaacgg aggaagcgcc
    17881 gcccgaaagc ggcgcgaaag gacttagctg aacagagagt agaagattgc ggagattgca
    17941 atcagaccca tcacgacaac gaatacgttg ctgatgtgac cgctgtactt acgcattgcc
    18001 ggtactttct gaattgcgta catcggcatc aggaacagga tcatcgcgat gattgggccg
    18061 cccagggttt caatcatacc caggatgctc gggttcaggg tggcaacaat ccaggtcgtt
    18121 accagcatga acagcgcagt gatacggttc agcttgttga tttcgataga cttaccttta
    18181 ccacgcagag atttaatcac cataccgttg aagccttcac gtgcgcccag gtagtggccg
    18241 aggaaggatt tggtgatagc gataatcgcg ataatcggag ccatccacgc gataaccggt
    18301 gcgttaaagt ggttagccag gtaagacaga atcgagatgt tctgctcttt agccgcagcc
    18361 aggtctgccg gagtcaggct cagtacgcag ctgaagacga agaacattac ggtcagcacc
    18421 atcatgatgt gtgcgaatgc caggatcttc gagcatttct gttctgccat atcgccgtac
    18481 tcttcacgct tcgcaacggc gaaagaagag atgatcggag agtggttgaa cgagaacacc
    18541 attaccggaa ttgccagcca cagggtcatc cacagaccgt ttccggttgc agatgcagtg
    18601 tccagagaca gcgtttccag tgctgcgccg ttccactgcg ggatcaggta cagagccagc
    18661 agcatcagta cgccaacaaa cgggaatacc agaatactca tcgctttaac gatcatctgc
    18721 tcaccaaagc gaacgatggt catcataccc acgatcagga tcagcgacag aatcgcacgc
    18781 ggcggtggcg tcatacccag ctggtgagac atgaagcttt caacggtatt ggtgattgcc
    18841 acgctataaa ccagcaggat cgggtagata gcgaagaagt agagcagggt aatcagttta
    18901 cctgcgccaa taccaaagtg ttcttctaca acctcggtga tgtcttcgcc cgggttttta
    18961 ccagacagta cgaagcgagt caggccgcgg tgagcaaaaa acgtcatcgg gaacgcaagg
    19021 atagccatga tgatcagcgg gatcataccg ccaacaccgg cgttgattgg caggaacagc
    19081 acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc acatggtgtc tgtcttgcgc
    19141 caggcactac gagagtcttt cgacgcaatc gtgctggttt gagtcgtttc catctatttc
    19201 tcctggagga atgcaaataa ttcagctttt agccagttca gcccgggaag gtgcctgacc
    19261 gtaaaaatga aatctgtcta tcggcgatgg cttaatcatt taccgccgta ataatttgag
    19321 ggcggaaaga tacacacaag ttatgtatct atcagtgatc ttgatcccaa atacaataat
    19381 ctgcttggaa gatagattat cccaacgctt tactctgtaa atcaaaagta aaagagaatt
    19441 taagggcaat atttgcccat taaacgacat ctgacttctt ccggggcgcg caatatatgt
    19501 aggttgaact gaaaaaatca atacttttag ttaatcatgg cgataatttc tttttcatag
    19561 cgataaaata cggtttatga aaatagggaa acgtttgtgt tgcaacataa attgtatgac
    19621 atattaactg tgatgatatt tgtcgcgtga gggtggtaag aaacattcat ataacaaatg
    19681 aaatgagttg atgagtaagt gaccaaaaac gagaaaaccc ggcaa
//
LOCUS       DADLEE010000077        19698 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000077 DADLEE010000000
VERSION     DADLEE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.077"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="JQH51_002871"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="JQH51_002871"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY6077151.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="JQH51_002872"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="JQH51_002872"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY6077152.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /locus_tag="JQH51_002873"
     CDS             complement(4495..5796)
                     /locus_tag="JQH51_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY6077153.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="JQH51_002874"
     CDS             6038..6658
                     /locus_tag="JQH51_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY6077154.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="JQH51_002875"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="JQH51_002875"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY6077155.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="JQH51_002876"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="JQH51_002876"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY6077156.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="JQH51_002877"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="JQH51_002877"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY6077157.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="JQH51_002878"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="JQH51_002878"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY6077158.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="JQH51_002879"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="JQH51_002879"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="JQH51_002880"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="JQH51_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="JQH51_002881"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="JQH51_002881"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY6077159.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /locus_tag="JQH51_002882"
     CDS             12769..14232
                     /locus_tag="JQH51_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HAY6077160.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="JQH51_002883"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="JQH51_002883"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY6077161.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="JQH51_002884"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="JQH51_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY6077162.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="JQH51_002885"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="JQH51_002885"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY6077163.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="JQH51_002886"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="JQH51_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY6077164.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4684 a         5506 c         5030 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggaac gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DADLEE010000078        19346 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000078 DADLEE010000000
VERSION     DADLEE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19346
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.078"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="JQH51_002887"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="JQH51_002887"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY6077165.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="JQH51_002888"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="JQH51_002888"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY6077166.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="JQH51_002889"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="JQH51_002889"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY6077167.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="JQH51_002890"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="JQH51_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY6077168.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="JQH51_002891"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="JQH51_002891"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY6077169.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="JQH51_002892"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="JQH51_002892"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY6077170.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /locus_tag="JQH51_002893"
     CDS             7827..8447
                     /locus_tag="JQH51_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY6077171.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="JQH51_002894"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="JQH51_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY6077172.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="JQH51_002895"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="JQH51_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY6077173.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="JQH51_002896"
     CDS             11296..11511
                     /locus_tag="JQH51_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077174.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="JQH51_002897"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="JQH51_002897"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY6077175.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="JQH51_002898"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="JQH51_002898"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY6077176.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="JQH51_002899"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="JQH51_002899"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY6077177.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="JQH51_002900"
     CDS             complement(16247..16786)
                     /locus_tag="JQH51_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077178.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="JQH51_002901"
     CDS             complement(16802..17320)
                     /locus_tag="JQH51_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY6077179.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="JQH51_002902"
     CDS             complement(17631..17822)
                     /locus_tag="JQH51_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY6077180.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="JQH51_002903"
     CDS             17840..17992
                     /locus_tag="JQH51_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077181.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..19313
                     /gene="pdeF"
                     /locus_tag="JQH51_002904"
                     /pseudo
     CDS             18174..19313
                     /gene="pdeF"
                     /locus_tag="JQH51_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT         4555 a         4708 c         5260 g         4823 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaatat gaggtgtact
    19321 ggcaatagcg gacactacca tttgtt
//
LOCUS       DADLEE010000079        18803 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000079 DADLEE010000000
VERSION     DADLEE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.079"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(139..3654)
                     /locus_tag="JQH51_002905"
     CDS             complement(139..3654)
                     /locus_tag="JQH51_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAY6077182.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="JQH51_002906"
     CDS             complement(4107..5369)
                     /locus_tag="JQH51_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY6077183.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="JQH51_002907"
     tRNA            complement(5567..5642)
                     /locus_tag="JQH51_002907"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="JQH51_002908"
     CDS             complement(5748..6455)
                     /locus_tag="JQH51_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY6077184.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /locus_tag="JQH51_002909"
     CDS             6853..8988
                     /locus_tag="JQH51_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY6077185.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /locus_tag="JQH51_002910"
     CDS             complement(9037..10293)
                     /locus_tag="JQH51_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY6077186.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="JQH51_002911"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="JQH51_002911"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY6077187.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="JQH51_002912"
     CDS             complement(11639..11914)
                     /locus_tag="JQH51_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY6077188.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="JQH51_002913"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="JQH51_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY6077189.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="JQH51_002914"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="JQH51_002914"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY6077190.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="JQH51_002915"
     CDS             13874..14200
                     /locus_tag="JQH51_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY6077191.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="JQH51_002916"
     CDS             14384..15103
                     /locus_tag="JQH51_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY6077192.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="JQH51_002917"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="JQH51_002917"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY6077193.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="JQH51_002918"
     CDS             16442..17404
                     /locus_tag="JQH51_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY6077194.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..18764)
                     /locus_tag="JQH51_002919"
     CDS             complement(17505..18764)
                     /locus_tag="JQH51_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY6077195.1"
                     /translation="MLVFIGVPISFSIGIATVASMLLMFPWDIAAITVSQRLANGLDN
                     FALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISGSA
                     VAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVSVA
                     SLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLLVFIV
                     MGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGF
                     SVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPI
                     FLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLLFY
                     AALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4898 a         4584 c         4645 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttggccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DADLEE010000080        18068 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000080 DADLEE010000000
VERSION     DADLEE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.080"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            256..780
                     /locus_tag="JQH51_002920"
     CDS             256..780
                     /locus_tag="JQH51_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6077196.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="JQH51_002921"
     CDS             861..1535
                     /locus_tag="JQH51_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY6077197.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4013
                     /locus_tag="JQH51_002922"
     CDS             1551..4013
                     /locus_tag="JQH51_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6077198.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4029..5062
                     /locus_tag="JQH51_002923"
                     /pseudo
     CDS             4029..5062
                     /locus_tag="JQH51_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="JQH51_002924"
     CDS             complement(5144..5482)
                     /gene="rcnB"
                     /locus_tag="JQH51_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY6077199.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5701..6531)
                     /locus_tag="JQH51_002925"
                     /pseudo
     CDS             complement(5701..6531)
                     /locus_tag="JQH51_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6653..6925
                     /gene="rcnR"
                     /locus_tag="JQH51_002926"
     CDS             6653..6925
                     /gene="rcnR"
                     /locus_tag="JQH51_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY6077200.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7017..7113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7148..7936
                     /gene="thiM"
                     /locus_tag="JQH51_002927"
     CDS             7148..7936
                     /gene="thiM"
                     /locus_tag="JQH51_002927"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY6077201.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7933..8733
                     /gene="thiD"
                     /locus_tag="JQH51_002928"
     CDS             7933..8733
                     /gene="thiD"
                     /locus_tag="JQH51_002928"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY6077202.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8798..9619
                     /locus_tag="JQH51_002929"
     CDS             8798..9619
                     /locus_tag="JQH51_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY6077203.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTEKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9667..10413
                     /locus_tag="JQH51_002930"
     CDS             9667..10413
                     /locus_tag="JQH51_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6077204.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10387..11352)
                     /locus_tag="JQH51_002931"
     CDS             complement(10387..11352)
                     /locus_tag="JQH51_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY6077205.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11349..12352)
                     /locus_tag="JQH51_002932"
                     /pseudo
     CDS             complement(11349..12352)
                     /locus_tag="JQH51_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12392..13444
                     /gene="fbaB"
                     /locus_tag="JQH51_002933"
     CDS             12392..13444
                     /gene="fbaB"
                     /locus_tag="JQH51_002933"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY6077206.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13752..14606
                     /gene="gatY"
                     /locus_tag="JQH51_002934"
     CDS             13752..14606
                     /gene="gatY"
                     /locus_tag="JQH51_002934"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY6077207.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14635..15897
                     /gene="gatZ"
                     /locus_tag="JQH51_002935"
     CDS             14635..15897
                     /gene="gatZ"
                     /locus_tag="JQH51_002935"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY6077208.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15907..16359
                     /gene="gatA"
                     /locus_tag="JQH51_002936"
     CDS             15907..16359
                     /gene="gatA"
                     /locus_tag="JQH51_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY6077209.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16390..16674
                     /gene="gatB"
                     /locus_tag="JQH51_002937"
     CDS             16390..16674
                     /gene="gatB"
                     /locus_tag="JQH51_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY6077210.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16678..18033
                     /locus_tag="JQH51_002938"
     CDS             16678..18033
                     /locus_tag="JQH51_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY6077211.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4728 a         4111 c         4570 g         4659 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2641 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2701 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2761 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2821 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2881 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2941 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     3001 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     3061 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     3121 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     3181 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     3241 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     3301 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     3361 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     3421 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3481 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3541 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3601 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3661 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3721 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3781 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3841 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3901 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3961 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     4021 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     4081 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     4141 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     4201 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     4261 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     4321 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     4381 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     4441 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4501 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4561 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4621 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4681 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4741 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4801 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4861 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4921 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4981 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     5041 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     5101 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     5161 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     5221 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     5281 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     5341 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     5401 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     5461 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5521 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5581 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5641 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5701 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5761 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5821 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5881 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5941 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     6001 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     6061 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     6121 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     6181 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     6241 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     6301 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     6361 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     6421 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6481 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6541 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6601 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6661 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6721 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6781 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6841 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6901 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6961 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     7021 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     7081 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     7141 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     7201 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     7261 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     7321 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     7381 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     7441 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7501 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7561 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7621 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7681 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7741 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7801 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7861 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7921 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7981 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     8041 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     8101 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     8161 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     8221 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     8281 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     8341 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     8401 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     8461 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8521 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8581 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8641 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8701 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8761 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8821 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8881 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8941 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     9001 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     9061 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     9121 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     9181 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     9241 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     9301 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     9361 ggaaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     9421 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9481 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9541 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9601 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9661 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9721 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9781 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9841 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9901 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9961 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    10021 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
    10081 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    10141 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    10201 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    10261 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    10321 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    10381 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    10441 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10501 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10561 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10621 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10681 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10741 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10801 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10861 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10921 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10981 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    11041 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    11101 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    11161 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    11221 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    11281 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    11341 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    11401 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    11461 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11521 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11581 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11641 tgttcgcggg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11701 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11761 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11821 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11881 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11941 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    12001 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    12061 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    12121 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    12181 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    12241 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    12301 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    12361 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    12421 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12481 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12541 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12601 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12661 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12721 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12781 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12841 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12901 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12961 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    13021 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    13081 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    13141 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    13201 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    13261 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    13321 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    13381 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    13441 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13501 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13561 cgtatgttta caccgcatcc ggcataaaaa tacgcgcact ttgctacggc ttccctatcg
    13621 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13681 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13741 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13801 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13861 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13921 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13981 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    14041 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    14101 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    14161 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    14221 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    14281 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    14341 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    14401 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    14461 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14521 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14581 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14641 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
    14701 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
    14761 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
    14821 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
    14881 attctcggcg gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg
    14941 atggaaaaat ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat
    15001 cttgatgcgt caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg
    15061 gaacgagctg ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag
    15121 caactgagct atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt
    15181 cagtcagtac acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag
    15241 gcctttattg cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag
    15301 ccgggtgtgg aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggca
    15361 ctggcgcaat ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag
    15421 acccggacgg cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc
    15481 gcattaacct ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt
    15541 atcgcccccg aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa
    15601 ccgcaatact ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt
    15661 tacagcctgt cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt
    15721 gaaacgatga tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat
    15781 cttcccaaac aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt
    15841 attatgaata aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga
    15901 cggtatatga ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt
    15961 ttaacctata tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag
    16021 gcattaattg ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt
    16081 gcaataccgc attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca
    16141 acaaataaag ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt
    16201 attgcgttga ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc
    16261 aagttacaac agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag
    16321 gaatacttca caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa
    16381 ggactattta tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg
    16441 atggcggcgg aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc
    16501 cagtgtcggg ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact
    16561 gccagggtgg atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct
    16621 ggtgtcggta tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg
    16681 ttttcagaag tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc
    16741 atcattattt tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg
    16801 catatcggga ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt
    16861 ggtccggcgg cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc
    16921 ggctggccgg gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg
    16981 attgcgatat tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt
    17041 gatatctgga atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt
    17101 tcatggatga tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc
    17161 gactggtttg cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac
    17221 ggtacgtcgg cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc
    17281 ccaggcgtta accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt
    17341 ggcgaacctg tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac
    17401 gatgtcaaag gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca
    17461 cggatgatta aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt
    17521 ttacaggcga agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg
    17581 gggcatacgg cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct
    17641 gtttgtgttc cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc
    17701 gtggcgatgg cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc
    17761 atcattatga gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg
    17821 gcggctaatg ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt
    17881 tctcccatta cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt
    17941 attatcggcg caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt
    18001 actaaacaag agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatcccc
    18061 cttaacaa
//
LOCUS       DADLEE010000081        17916 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000081 DADLEE010000000
VERSION     DADLEE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17916
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.081"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            414..995
                     /locus_tag="JQH51_002939"
     CDS             414..995
                     /locus_tag="JQH51_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY6077212.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            1039..5701
                     /locus_tag="JQH51_002940"
                     /pseudo
     CDS             1039..5701
                     /locus_tag="JQH51_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            6069..7724
                     /gene="lldP"
                     /locus_tag="JQH51_002941"
     CDS             6069..7724
                     /gene="lldP"
                     /locus_tag="JQH51_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY6077213.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7724..8500
                     /gene="lldR"
                     /locus_tag="JQH51_002942"
     CDS             7724..8500
                     /gene="lldR"
                     /locus_tag="JQH51_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY6077214.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8497..9687
                     /gene="lldD"
                     /locus_tag="JQH51_002943"
     CDS             8497..9687
                     /gene="lldD"
                     /locus_tag="JQH51_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY6077215.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9883..10356
                     /gene="trmL"
                     /locus_tag="JQH51_002944"
     CDS             9883..10356
                     /gene="trmL"
                     /locus_tag="JQH51_002944"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY6077216.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10409..11230)
                     /gene="cysE"
                     /locus_tag="JQH51_002945"
     CDS             complement(10409..11230)
                     /gene="cysE"
                     /locus_tag="JQH51_002945"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY6077217.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11310..12329)
                     /gene="gpsA"
                     /locus_tag="JQH51_002946"
     CDS             complement(11310..12329)
                     /gene="gpsA"
                     /locus_tag="JQH51_002946"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY6077218.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12329..12796)
                     /gene="secB"
                     /locus_tag="JQH51_002947"
     CDS             complement(12329..12796)
                     /gene="secB"
                     /locus_tag="JQH51_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY6077219.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12859..13110)
                     /gene="grxC"
                     /locus_tag="JQH51_002948"
     CDS             complement(12859..13110)
                     /gene="grxC"
                     /locus_tag="JQH51_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY6077220.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13252..13683)
                     /locus_tag="JQH51_002949"
     CDS             complement(13252..13683)
                     /locus_tag="JQH51_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY6077221.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13928..15472
                     /gene="gpmM"
                     /locus_tag="JQH51_002950"
                     /gene_synonym="pgmI"
     CDS             13928..15472
                     /gene="gpmM"
                     /locus_tag="JQH51_002950"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY6077222.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15482..16765
                     /gene="envC"
                     /locus_tag="JQH51_002951"
     CDS             15482..16765
                     /gene="envC"
                     /locus_tag="JQH51_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY6077223.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16769..17728
                     /locus_tag="JQH51_002952"
     CDS             16769..17728
                     /locus_tag="JQH51_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY6077224.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17715..17916)
                     /locus_tag="JQH51_002953"
                     /pseudo
     CDS             complement(17715..17916)
                     /locus_tag="JQH51_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4501 a         4639 c         4660 g         4116 t
ORIGIN      
        1 tttcggattt aattgtgaaa ttttaatcat catattcaca aaatcaatta tctgtgaaat
       61 ttttcccaga catggtttct ttcatatttg acatttatta ataatcatca ttagaatcca
      121 cgaaaaataa tatattcata caaacacaga catcttttga aacgaatgat acatttttat
      181 gatgaatgct taaaacaata tttaatcgac acacatcact gtcattaatg taaagcaaca
      241 tagctcacca ataatacaat ggacaactct accggcattg agcgctggtc ggaggaaata
      301 aggattttta catgaacttg aagaagattt ttttcagtgc tgtcactgtt tcagttctct
      361 gcgcattaac gggatgtgac tacattgaag aaggcaagcc agaaagtagt ttactgaaac
      421 aacaagaaga acataataat aaaattgtgc tacttgaaaa acaacaagca cagctaaaga
      481 gtcaactcga aactattcaa aaacaacaaa ctggcataat aaacagtaca aagactttaa
      541 ctcacgtaat taaatccgtt aaagaccaac aaaatacttt tatttttacc gagtttaacc
      601 cagcaaaaac gaagtatttc atcctgaaca acggttcggt ggctttagcg ggtcgagtgt
      661 tatccattga cgccacagag aatggtagtg ttattcatat ctcactggtc aacttattaa
      721 gtacacctat ctcaaacatt ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg
      781 ccaaagagtt tgcccggtgg caacaattac ttttcaacac atcaatgaaa tccacattga
      841 aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta tcgcccaata
      901 acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac aatcttccct
      961 ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc ttataacaag
     1021 gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc tgcgacaggg
     1081 aattatactg ttaccagcga aacggcaaaa agccgtggca agaaatctgg gcgcagtaag
     1141 ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc attggcgaat
     1201 gccgggaatg acaacggtca gggtgttgat tacggtagtg gatcagctgg cgacggctgg
     1261 gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg ttccagtact
     1321 gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg gtcaaaaacc
     1381 catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga aggcgataga
     1441 agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc cctcggccgt
     1501 tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa agcggaagga
     1561 gcaaacgcca ttgccctggg gcgtagcagt gtagctagcg gtactgacag cctcgcattt
     1621 ggcagacaat cacttgccag cgcagcgaac gctattgcga taggtgctga gaccgaagcc
     1681 gctgaaaatg caactgctat tggcaataat gcgaaggcaa aagggactaa tagcatggca
     1741 atggggttcg gaagccttgc cgataaagtc aatactatcg cattaggaaa tggcagccag
     1801 gctctggcag ataatgcaat cgccataggc cagggcaaca aagctgatgg cgtggatgcc
     1861 atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt aggcacagcc
     1921 agtaatgcaa ctggtgataa gagtcttgcg cttggtagta atagcagtgc caacggtatt
     1981 aactctgtcg cgctgggcgc agattccatt gcggatttag acaataccgt ctctgtcggc
     2041 aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa gtctgacagt
     2101 tacgatgcca ttaatggttc acagctttat gccattagcg actcggtagc aaaaaaggct
     2161 tggaggaggg gctgcagtag atgttgatga cggtactgtt acagcaccaa cctacaattt
     2221 aaaaaatggt agcaaaaata acgtaggggc tgcgctcgct gtacttgatg aaaacaccct
     2281 gcaatgggac caaaccaaag gcaaatacag cgctgctcat ggtactagta gcccaactgc
     2341 cagcgtaatc accgatgttg cggatggcac gatttcagcc tccagtaagg atgcggttaa
     2401 cggttcccaa ctgaaagcta ccaatgacga tgtcgaagcc aacaccacca atatcaccaa
     2461 cctgacggat tccgttggtg accttcaggc tgatgccctg ctctggaacg aaactaaaaa
     2521 ggcattcagt gcagctcacg gccaggatac caccagcaaa atcaccaacg ttaaagatgc
     2581 cgacctgacg gctgacagca ctgatgctgt taacggctct cagctgaaaa ccaccaacga
     2641 tgctgtggcg acgaatacca ccaatatcgc caataacact tccaatattg ccactaacac
     2701 caccaacatc tctaacctga ctgagacggt gactaatctt ggtgaggatg cgctgaaatg
     2761 ggataaggac aatggtgtat tcacggcagc tcatggcacc gagaccacca gcaaaataac
     2821 caacgttaaa gatggcgacc tgacgactgg cagcaccgat gccgttaacg gctctcagct
     2881 gaaaaccacc aacgatgccg tggcgacgaa taccaccaat atcgccacta acaccaccaa
     2941 catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa
     3001 ggacaatggt gtcttcactg cagctcatgg caacaatacc gccagcaaaa tcaccaatat
     3061 cctggacggc acagtcactg caaccagttc cgatgccatt aacggtagcc agctttatga
     3121 cttaagcagc aatatcgcca cctacttcgg cggcaatgct tctgtgaata ctgacggtgt
     3181 gtttaccggt ccaacctaca aaatcggtga aacaaattat tataacgtcg gcgatgcact
     3241 ggctgcgatt aactcctcat ttagcacgtc tctcggcgat gctctgcttt gggatgccac
     3301 cgcaggtaaa ttcagtgcca aacacggtac taatggtgac gcaagcgtga tcactgatgt
     3361 cgcagatggt gaaatttcag actccagttc tgacgcagta aacggctcac aactccacgg
     3421 cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg cagacggcac
     3481 catcactgcg ccgacgtaca ccattgctaa tgctgattac gataatgtcg gtgatgccct
     3541 gaatgctatc gataccactc ctgacgacgc tctgctctgg gatgcggacg ccggtgaaaa
     3601 tggtgcattt agcgccgctc acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc
     3661 taacggtgca atctctgctg ccagcagcga cgcgattaac ggctcacaac tctataccac
     3721 caataagtac atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat
     3781 caccgcaccg acttacacca ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga
     3841 tgcgcttgat gataacgccc tgctgtggga taagactgcc aatggcggtg ctggagccta
     3901 caatgccagc catgacggta aagccagcat catcactaat gtcgctaatg gcagtattag
     3961 tgaggacagt accgatgcag tgaacggttc tcagttgaat gcgacgaata tgatgattga
     4021 gcagaacacc caaattatca atcagctcgc tggtaacacc gacgcaacct atatcgaaga
     4081 aaatggcgct ggtatcaact acgttcgtac taacgacaac gatttagcct ttaacgatgc
     4141 aagcgcctct ggtgtcggcg ctacagctgt aggttataac gctgtcgcgt ctggtgccag
     4201 cagcgtagcc attggtcaga acagcagcag caccgttgat accggtattg cgctgggtag
     4261 cagctccgtt tccagccgtg tgattgccaa aggttctcgt gacactagcg taacggaaaa
     4321 tggcgtggtg attggttatg acaccactga cggcgaactg ctaggtgcat tgtcaattgg
     4381 tgatgacggt aaataccgcc aaatcatcaa cgtagctgat ggttcagaag cccatgacgc
     4441 cgttacggtt cgccagttgc agaacgctat tggagcggtc gccactacgc caaccaagta
     4501 ctttcacgcc aactcaacgg cagaagactc actggccgtt ggtgaagact cgctggcaat
     4561 gggtgcgaaa actgtcgtta atggtaatgc aggtattggt atcggtttga acactctggt
     4621 tctggctgat gcgatcaacg gcattgctat cggcagtaac gcacgcgcaa accatgcaaa
     4681 cagtatcgca atgggtaacg gttctcagac cacccgtggt gcacagactg gctacaccgc
     4741 ctacaacatg gacgcaccgc agaactctgt aggtgagttt tctgtcggta gtgaagacgg
     4801 tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat accgatgcgg ttaacgtggg
     4861 tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc cagagcatta ctaacctgaa
     4921 caatcaggtc actaatctgg atactcgcgt tactaatatc gaaaacggta ttggcgacat
     4981 tgtcaccacc ggtagcacca agtacttcaa gaccaacacc gatggcgtag atgccaacgc
     5041 ccagggtaaa gatagcgttg ctattggttc tggttccatt gctgccgctg acaacagcgt
     5101 cgcactgggt accggttccg ttgcagagga agaaaataca atctctgtag gttcttctac
     5161 taaccaacgc cgtatcacca acgtagctgc cagtgttaat gccaccgatg cggttaacgt
     5221 gtcgcagctg aaatcttctg aagcaggcgg agtgcgctac gacaccaaag ctgatggttc
     5281 tatcgactat agcaatatca ccctcggtgg tggcaacggc agtacgactc gtatcagcaa
     5341 cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac gcgcagttga agcaaagcgc
     5401 gcaggaaacg aagcaataca ccgatcagcg gatggttgag atggataaca aactgtctaa
     5461 aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca
     5521 ggcttataca ccgggtgcca gcatggcttc tattggtggc ggtacttaca acggtgaatc
     5581 ggcagttgct ttaggtgtat cgatggtgag cgccaatggt cgttgggtct acaaattaca
     5641 aggtagtacc aatagccagg gtgaatactc cgccgcactc ggtgccggta ttcagtggta
     5701 atgatccatt aagttagtgt gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc
     5761 atcaggcaag ataaggcgct ccgggtcaac aatctaaggg caattctctg atgaggattg
     5821 cccttttctt taccagacat ctccccccac aagaattggc cctaccaatt cttcgcttat
     5881 ctgacctctg gttcacaatt ccccaattaa aactcacatc aatattgcca atacataaca
     5941 tttagttaac cattcattgt cattatccct acacaacaca attggcagtg ccacttttac
     6001 acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactctagga
     6061 gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca
     6121 gtctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactga
     6181 aaggatacgt cgccgcctca tggacggtag caatcgccct tgccgtggct ttgctgttct
     6241 ataaaatgcc ggtcgctaac gcgctggcct cggtggttta tggcttcttc tatggtttgt
     6301 ggcccatcgc gtggatcatt attgcagcag tgttcgtcta taagatctcg gtgaaaaccg
     6361 ggcagtttga catcattcgc tcgtctattc tttcgataac ccctgaccag cgtctgcaaa
     6421 tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg agccgcaggc tttggcgcac
     6481 cggtagcaat taccgccgca ttgctggtcg gcctgggttt taaaccgctg tacgccgccg
     6541 gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggc attccaatcc
     6601 tggttgccgg acaagtaaca ggcatcgaca gctttgagat tggccagatg gtggggcgtc
     6661 agctaccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggctggc
     6721 gcggtatcaa agagacgtgg cctgcggtcg tggttgcagg cggctcgttt gccatcgctc
     6781 agtaccttag ctctaacttc attgggccgg agctgccgga cattatctct tcgctggtat
     6841 cactgctctg cctgacgctg ttcctcaaac gctggcaacc agttcgcgta ttccgcttcg
     6901 gtgacttggg ggcgtcacag gttgatatga cgctggccca caccggttac accgcgggtc
     6961 aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca ctgtggagca
     7021 tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca
     7081 ttccggtgcc gtacctcaat aaactggttg cccgtatgcc accagtggtc agcgaggcta
     7141 cagcctatgc cgccgtgttt aagtttgact ggttctctgc caccggcacc gccattctgt
     7201 ttgctgcact gctctcgatt gtctggctga agatgaaacc gtctgacgct atcagcacct
     7261 tcggcagcac gctgaaagaa ctggctctac ccatctactc catcggcatg gtgctggcgt
     7321 tcgcctttat ctcgaactat tccggactgt catcaacact ggcactggca ctggcgcaca
     7381 ccggtcatgc attcaccttc ttctcgccgt tcctcggctg gctgggggta ttcctgaccg
     7441 ggtcggatac ctcatctaac gccctgttcg ccgcgctgca agccaccgca gcacaacaaa
     7501 ttggcgtctc tgatctgttg ctggttgccg ccaataccac cggtggcgtc accggtaaga
     7561 tgatctcccc gcaatctatc gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt
     7621 ctgatttgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga
     7681 tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag
     7741 acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga
     7801 agcgggcatg aagttgcccg ctgagcgcca actggcgatg caactcggcg tatcacgtaa
     7861 ttcactacgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg
     7921 cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc
     7981 gctaaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta
     8041 cgccattgaa gccagcaccg catggcatgc ggcaatgcgc gccacacctg gcgacaaaga
     8101 aaagattcag ctttgctttg aagcaacgct aagtgaagac ccggatatcg cctcacaagc
     8161 ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac
     8221 catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc agcggatgta
     8281 tctggtgcca ccggtttttt cacaactgac cgaacaacat caggctgtca ttgacgccat
     8341 ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta gttttgttca
     8401 caccaccatg aaacgattcg atgaagatca ggctcgccac gcacggatta cccgcctgcc
     8461 cggtgagcat aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat
     8521 tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt
     8581 gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc
     8641 cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa
     8701 ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc
     8761 gaagttcagg cagccaaagc ggcggacgcg catggcattc cgtttactct ctcgacggtt
     8821 tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt
     8881 tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt
     8941 tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg
     9001 cattcaggta tgagcggccc aaatgcggca atgcgccgct acttgcaggc ggtgacgcat
     9061 ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctca
     9121 gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc
     9181 gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga tggcccgatg
     9241 gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat
     9301 ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt
     9361 gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga
     9421 attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg
     9481 ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta
     9541 aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagt
     9601 gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctac ggcactggct
     9661 ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa ccctctccct
     9721 cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact
     9781 ctttttttct ccctcgcccc tccggggagg gccggggtga ggggaaaagg ccgcacggtt
     9841 caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa catcgtactt
     9901 tacgaaccag aaattccgcc aaatactggc aacatcatcc gtctttgcgc taataccggc
     9961 tttcgtctac atatcatcga accgatggga tttgcctggg acgacaagcg cctgcgccgc
    10021 gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgacta tcgcgcgttc
    10081 ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac gcctgctcac
    10141 agcgccgtaa gctatcagga tggcgattat ctgatgttcg gcccggaaac acgcggcctg
    10201 ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc gatggtgccg
    10261 gacagccgca gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg
    10321 cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg atagatgatt
    10381 acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca aatgtatggt
    10441 taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct ggtttaccga
    10501 caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc agcaccacgg
    10561 aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt ttcgcgcccg
    10621 cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat ttacccgtac
    10681 cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc gtttcaccca
    10741 caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct gccgggtgaa
    10801 tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat gcgcgacgcc
    10861 cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt
    10921 ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat
    10981 cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttca accacttcac
    11041 ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg tagctcagtg
    11101 cactgccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc agcattggct
    11161 cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt tccagttctt
    11221 cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg cgcgggccgg
    11281 gtcatagcgg taacaaaggt tccctggggt tagtgactgc tgcgctcgtc cttgcgtgca
    11341 cgacctaata atgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa tacttgataa
    11401 atttcctcgg ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc
    11461 gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg tacatccatg
    11521 ccctgaccga gcatcatacc aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc
    11581 accagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc cagcgccgca
    11641 ccaagacgcg acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca
    11701 aaaccgatac cgtcagacat ccccgcgcca atggcaatga cgtttttcac cgcgccgcca
    11761 agctgcacgc caatgaaatc cgggttgctg taaacgcgga aacttttgcc gcagtgcagc
    11821 agctgctgga gatcatcggc aaaggtctga tcggtcgagg ccagcgaaat agctgtcggt
    11881 aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag cggaatttga
    11941 tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc ttccagccct
    12001 ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg gcgcagcact
    12061 tcaccaaaga catggctggg tacgacgacg agaatattac gactggctgc cagcgcagtg
    12121 gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag aaacgcggcg
    12181 ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca gaggacaacc
    12241 tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc
    12301 acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca gtaccttcgc
    12361 cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca
    12421 gttgcgggaa tgtaccgcgg gataccatgc tggtgatgca ctcacgagca tacgggaaca
    12481 gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg atacccgcga
    12541 tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg cccaaggagg
    12601 ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg gaagatgccg
    12661 tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg
    12721 gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc atttcagtgt
    12781 tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt
    12841 aataccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg catccaatgc
    12901 atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt
    12961 gcaaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga tcggcaactc
    13021 ctggaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat acgggcaggt
    13081 ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt accctgaaat
    13141 ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac aataagtcgc
    13201 tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc
    13261 gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca gcacgaatac
    13321 ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc
    13381 gtctaccacg ataaccgatt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt
    13441 gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga agtcatcacg
    13501 ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg
    13561 agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag
    13621 taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg
    13681 catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc
    13741 gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcacgacgaa
    13801 aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg atcttcagag
    13861 gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt
    13921 tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta
    13981 tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact
    14041 gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg gtctgcctga
    14101 ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta
    14161 tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccagt
    14221 gctggctggc gcggtacata aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct
    14281 gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg tagaactggc
    14341 agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc gcgacacccc
    14401 gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa
    14461 aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga
    14521 tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagttcc aggccgatac
    14581 tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc
    14641 gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcgctgat
    14701 tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga
    14761 tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata
    14821 cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta acaccttcgg
    14881 cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc
    14941 ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat
    15001 tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga
    15061 gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca ttatctgtaa
    15121 ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg ttaaagcggt
    15181 tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca
    15241 actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca
    15301 ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt
    15361 gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg
    15421 tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc
    15481 catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat
    15541 caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc
    15601 ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgccg caaaagagcg
    15661 cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga
    15721 agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact
    15781 caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc
    15841 ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg agcataccgg
    15901 tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac aggcttattt
    15961 cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga
    16021 agtcaccatg cagcatgctg aactggaaga gaaacaaagc gagcaacaaa cgctgttata
    16081 tgaacagcgc gcccaacagg cgaaactgac tcaggctctg aacgagcgta aaaagacgct
    16141 ggcggggctg gaatcttcca tccagcaagg tcagcagcag ctaagtgaac tgcgtgccaa
    16201 cgaatcccgt ctgcgtaaca gcattgcccg tgctgaagcc gcggcgaaag cgcgtgctga
    16261 acgagaagcg cgtgaggccc aggcggttcg cgagcgccag aaagaagcga cgcgcaaagg
    16321 caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg
    16381 tgcgccgcgt ggtcaggcat tctggccggt tcgcgggccg acgctgcatc gctatggcga
    16441 gcagctacag ggtgaattac gctggaaagg gatggttatc ggtgcctctg aaggtactga
    16501 agttaaagca attgccgacg gtcgagtgat tctggctgac tggctgcaag gttacggtct
    16561 ggtggtggtg gttgagcacg gtaaaggcga catgagtctt tacggctata atcagagcgc
    16621 actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag
    16681 tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa
    16741 tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt
    16801 tgccgctctg ttggcgctct cctcccccgt acttgctggc aaacttgcca tcgtcattga
    16861 tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc
    16921 cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag
    16981 cggtcacgaa gtgttgattc atctcccgat ggcaccgttg agtaaacagc cgctggagaa
    17041 aaatacgcta cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt
    17101 caataacgtg ccctatgccg tggggatcaa caaccacatg ggcagcaaga tgacctctaa
    17161 cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt acttcctcga
    17221 cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggtactg gcgtgaaggt
    17281 gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt
    17341 taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca
    17401 cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct
    17461 ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acttctacac cgccgaaaaa
    17521 cgctgtgcct gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa
    17581 accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca
    17641 gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc
    17701 ggcgaagttt aacgctagcg caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc
    17761 gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctggta accgaccttc
    17821 acgctgcgca cattggtaat caggcgctta tacatacccg gaagtaaata tctcggcaat
    17881 catccgttgg cgggtaagaa tatccggctc tttgcg
//
LOCUS       DADLEE010000082        17749 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000082 DADLEE010000000
VERSION     DADLEE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17749
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.082"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            131..574
                     /locus_tag="JQH51_002954"
     CDS             131..574
                     /locus_tag="JQH51_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6077225.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /locus_tag="JQH51_002955"
     CDS             810..1628
                     /locus_tag="JQH51_002955"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY6077226.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQH51_002956"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQH51_002956"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY6077227.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQH51_002957"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQH51_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY6077228.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQH51_002958"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQH51_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6077229.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /locus_tag="JQH51_002959"
     CDS             complement(4319..4468)
                     /locus_tag="JQH51_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY6077230.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="JQH51_002960"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="JQH51_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY6077231.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="JQH51_002961"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="JQH51_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY6077232.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="JQH51_002962"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="JQH51_002962"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY6077233.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="JQH51_002963"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="JQH51_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY6077234.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="JQH51_002964"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="JQH51_002964"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY6077235.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="JQH51_002965"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="JQH51_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY6077236.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="JQH51_002966"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="JQH51_002966"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY6077237.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQH51_002967"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQH51_002967"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY6077238.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="JQH51_002968"
     CDS             13934..14314
                     /locus_tag="JQH51_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077239.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="JQH51_002969"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="JQH51_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY6077240.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /locus_tag="JQH51_002970"
     CDS             complement(15366..16085)
                     /locus_tag="JQH51_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY6077241.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQH51_002971"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQH51_002971"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY6077242.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="JQH51_002972"
     tRNA            complement(17415..17490)
                     /locus_tag="JQH51_002972"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="JQH51_002973"
     tRNA            complement(17494..17569)
                     /locus_tag="JQH51_002973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="JQH51_002974"
     tRNA            complement(17621..17696)
                     /locus_tag="JQH51_002974"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
BASE COUNT         4524 a         4637 c         4415 g         4173 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcaaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gagcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggt
//
LOCUS       DADLEE010000083        17647 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000083 DADLEE010000000
VERSION     DADLEE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.083"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="JQH51_002975"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="JQH51_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JQH51_002976"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JQH51_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY6077243.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JQH51_002977"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JQH51_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JQH51_002978"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JQH51_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY6077244.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JQH51_002979"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JQH51_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY6077245.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="JQH51_002980"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="JQH51_002980"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY6077246.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="JQH51_002981"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="JQH51_002981"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY6077247.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JQH51_002982"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JQH51_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY6077248.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JQH51_002983"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JQH51_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY6077249.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JQH51_002984"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JQH51_002984"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY6077250.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="JQH51_002985"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="JQH51_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="JQH51_002986"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="JQH51_002986"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY6077251.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="JQH51_002987"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="JQH51_002987"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY6077252.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="JQH51_002988"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="JQH51_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="JQH51_002989"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="JQH51_002989"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY6077253.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15326
                     /locus_tag="JQH51_002990"
     CDS             14613..15326
                     /locus_tag="JQH51_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY6077254.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            15331..16008
                     /gene="ydjY"
                     /locus_tag="JQH51_002991"
     CDS             15331..16008
                     /gene="ydjY"
                     /locus_tag="JQH51_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY6077255.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16023..16730
                     /locus_tag="JQH51_002992"
     CDS             16023..16730
                     /locus_tag="JQH51_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY6077256.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16730..17278
                     /locus_tag="JQH51_002993"
     CDS             16730..17278
                     /locus_tag="JQH51_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY6077257.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17288..17614
                     /locus_tag="JQH51_002994"
                     /pseudo
     CDS             17288..17614
                     /locus_tag="JQH51_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4366 a         4686 c         4309 g         4286 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggata ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagagctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactact ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac
    15301 ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac attattcagt gtcatggaaa
    15361 aaaggactgg ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa
    15421 gagaatgcgg cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgtc
    15481 gatgccaata accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac
    15541 gacactcgtc acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg
    15601 gcttatgcgc ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc
    15661 gaaaacatga cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt
    15721 tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt
    15781 aatggcaaga aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa
    15841 acgggttgcc tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac
    15901 acttatggtg cggtggaaaa acacggcgaa gtgaaattca aaggcaatgc ctcagttctc
    15961 ccggcggata acacgctggc aacggttacc tttaaaatca ccgaataaag ccaagataaa
    16021 ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt
    16081 tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca
    16141 gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg
    16201 gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc
    16261 tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct
    16321 tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga
    16381 tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt atggacggct
    16441 ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg gttattgcct tttgtccctt
    16501 tcgatccaat cagctatgct gccggtctga cttcaatacg ttttcgctcg ttttttatcg
    16561 ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcaggc agcatgttaa
    16621 caggcggtac tttctggttt gtcaccgaac tgtttattct gtttgccctg accgtggtga
    16681 tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgactga tgggtttacc
    16741 gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta
    16801 cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc ccgtttatct tttatttggt
    16861 gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc
    16921 gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag
    16981 tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg ctgactggcg
    17041 gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag
    17101 cgtcacaccg ccaacagtcg atgatgccct gcgtacccga ctggctgcgc attttgacgc
    17161 tcaggcgctc accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa
    17221 ttctgccatg gacattcccg ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg
    17281 agggaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctttggcaac
    17341 acatgccagt gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa
    17401 cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa
    17461 aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag acgcggtgaa
    17521 gcgcattcag accgaagctg ctgccggtcg taaaacgggc ggctcggtgg atctgctctg
    17581 ggtgaacggc gaaaacttcc gcaccttaaa agagtaatgc cagtcagtta agcaactgac
    17641 tggcatt
//
LOCUS       DADLEE010000084        17479 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000084 DADLEE010000000
VERSION     DADLEE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.084"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JQH51_002995"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JQH51_002995"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY6077258.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JQH51_002996"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JQH51_002996"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY6077259.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JQH51_002997"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JQH51_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY6077260.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JQH51_002998"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JQH51_002998"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY6077261.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="JQH51_002999"
     CDS             complement(5464..5661)
                     /locus_tag="JQH51_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY6077262.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JQH51_003000"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JQH51_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY6077263.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JQH51_003001"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JQH51_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY6077264.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JQH51_003002"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JQH51_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY6077265.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JQH51_003003"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JQH51_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY6077266.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JQH51_003004"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JQH51_003004"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY6077267.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JQH51_003005"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JQH51_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY6077268.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JQH51_003006"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JQH51_003006"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY6077269.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JQH51_003007"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JQH51_003007"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY6077270.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="JQH51_003008"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="JQH51_003008"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY6077271.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="JQH51_003009"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="JQH51_003009"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY6077272.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="JQH51_003010"
     CDS             complement(17102..17155)
                     /locus_tag="JQH51_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077273.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17389..17464)
                     /locus_tag="JQH51_003011"
     tRNA            complement(17389..17464)
                     /locus_tag="JQH51_003011"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17429..17431),aa:Gly,seq:gcc)
BASE COUNT         3960 a         4985 c         4396 g         4138 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttagccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactggatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaagaatttg gagcgggaaa
    17401 cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc
    17461 ccgcttgggt ggtctgtgc
//
LOCUS       DADLEE010000085        17265 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000085 DADLEE010000000
VERSION     DADLEE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.085"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="JQH51_003012"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="JQH51_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY6077274.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="JQH51_003013"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="JQH51_003013"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY6077275.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="JQH51_003014"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="JQH51_003014"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY6077276.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="JQH51_003015"
     CDS             complement(2205..2348)
                     /locus_tag="JQH51_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY6077277.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="JQH51_003016"
     CDS             complement(3019..3306)
                     /locus_tag="JQH51_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077278.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="JQH51_003017"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="JQH51_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY6077279.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="JQH51_003018"
     CDS             3683..3922
                     /locus_tag="JQH51_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077280.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="JQH51_003019"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="JQH51_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY6077281.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="JQH51_003020"
     CDS             5033..6406
                     /locus_tag="JQH51_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077282.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="JQH51_003021"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="JQH51_003021"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY6077283.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="JQH51_003022"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="JQH51_003022"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY6077284.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="JQH51_003023"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="JQH51_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY6077285.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /locus_tag="JQH51_003024"
     CDS             complement(10388..10885)
                     /locus_tag="JQH51_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY6077286.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="JQH51_003025"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="JQH51_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY6077287.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="JQH51_003026"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="JQH51_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="JQH51_003027"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="JQH51_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="JQH51_003028"
     CDS             16524..16760
                     /locus_tag="JQH51_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY6077288.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16781..17056
                     /locus_tag="JQH51_003029"
     CDS             16781..17056
                     /locus_tag="JQH51_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY6077289.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGSCFPIEDFIDGFWQPAQEYGDF"
     gene            complement(17081..>17265)
                     /locus_tag="JQH51_003030"
     CDS             complement(17081..>17265)
                     /locus_tag="JQH51_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY6077290.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         4188 a         4354 c         4160 g         4563 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatacgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    16981 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    17041 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    17101 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    17161 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    17221 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DADLEE010000086        17091 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000086 DADLEE010000000
VERSION     DADLEE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.086"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..510
                     /locus_tag="JQH51_003031"
     CDS             <1..510
                     /locus_tag="JQH51_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAY6077291.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="JQH51_003032"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="JQH51_003032"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY6077292.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="JQH51_003033"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="JQH51_003033"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY6077293.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="JQH51_003034"
     CDS             complement(2878..3075)
                     /locus_tag="JQH51_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY6077294.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="JQH51_003035"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="JQH51_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAY6077295.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="JQH51_003036"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="JQH51_003036"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY6077296.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="dhbA"
                     /locus_tag="JQH51_003037"
     CDS             complement(5959..6705)
                     /gene="dhbA"
                     /locus_tag="JQH51_003037"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY6077297.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="JQH51_003038"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="JQH51_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY6077298.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="JQH51_003039"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="JQH51_003039"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY6077299.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="JQH51_003040"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="JQH51_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="JQH51_003041"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="JQH51_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY6077300.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="JQH51_003042"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="JQH51_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY6077301.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="JQH51_003043"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="JQH51_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY6077302.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="JQH51_003044"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="JQH51_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY6077303.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="JQH51_003045"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="JQH51_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY6077304.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="JQH51_003046"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="JQH51_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4864 c         4443 g         3791 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgtgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta a
//
LOCUS       DADLEE010000087        16388 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000087 DADLEE010000000
VERSION     DADLEE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.087"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="JQH51_003047"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="JQH51_003047"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY6077305.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JQH51_003048"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JQH51_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY6077306.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="JQH51_003049"
     CDS             2339..2554
                     /locus_tag="JQH51_003049"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077307.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="JQH51_003050"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="JQH51_003050"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY6077308.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /locus_tag="JQH51_003051"
     CDS             4449..5843
                     /locus_tag="JQH51_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY6077309.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="JQH51_003052"
     CDS             5920..6417
                     /locus_tag="JQH51_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY6077310.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="JQH51_003053"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="JQH51_003053"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY6077311.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGRYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="JQH51_003054"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="JQH51_003054"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY6077312.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="JQH51_003055"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="JQH51_003055"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY6077313.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9030..9665
                     /locus_tag="JQH51_003056"
     CDS             9030..9665
                     /locus_tag="JQH51_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY6077314.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="JQH51_003057"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="JQH51_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY6077315.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="JQH51_003058"
     CDS             complement(10257..11237)
                     /locus_tag="JQH51_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY6077316.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="JQH51_003059"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="JQH51_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY6077317.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="JQH51_003060"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="JQH51_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY6077318.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="JQH51_003061"
     CDS             12540..12830
                     /locus_tag="JQH51_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY6077319.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="JQH51_003062"
     CDS             12838..13431
                     /locus_tag="JQH51_003062"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY6077320.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="JQH51_003063"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="JQH51_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY6077321.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="JQH51_003064"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="JQH51_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..16385
                     /locus_tag="JQH51_003065"
     CDS             15882..16385
                     /locus_tag="JQH51_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY6077322.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4057 a         4220 c         4125 g         3986 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc ggtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaatcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg gcgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 accccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccacgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atgccttgtg ttttattaat caaagcattc aacaactgaa acaacctgcg caggaggcca
    16381 gttaatgg
//
LOCUS       DADLEE010000088        15929 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000088 DADLEE010000000
VERSION     DADLEE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.088"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="JQH51_003066"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="JQH51_003066"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY6077323.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="JQH51_003067"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="JQH51_003067"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY6077324.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="JQH51_003068"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="JQH51_003068"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY6077325.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIERARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="JQH51_003069"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="JQH51_003069"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY6077326.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="JQH51_003070"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="JQH51_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="JQH51_003071"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="JQH51_003071"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY6077327.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="JQH51_003072"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="JQH51_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="JQH51_003073"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="JQH51_003073"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY6077328.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="JQH51_003074"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="JQH51_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY6077329.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="JQH51_003075"
     CDS             complement(8973..10211)
                     /locus_tag="JQH51_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY6077330.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGCSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="JQH51_003076"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="JQH51_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY6077331.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="JQH51_003077"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="JQH51_003077"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY6077332.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="JQH51_003078"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="JQH51_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY6077333.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="JQH51_003079"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="JQH51_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY6077334.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14681..15583
                     /locus_tag="JQH51_003080"
     CDS             14681..15583
                     /locus_tag="JQH51_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6077335.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15730..15929
                     /locus_tag="JQH51_003081"
                     /pseudo
     CDS             15730..15929
                     /locus_tag="JQH51_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3952 a         4120 c         3855 g         4002 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgagcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgcagccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaacttaat
    14581 cctgagttaa gcaaaaaaac ataatcaata aaaatacata cgcaaaaaac attgattaag
    14641 tgaatatacc atggaagaaa aatataaacc ggagtcgtgt atggccaatc tctacgactt
    14701 gaaaaagttc gacttaaatc ttcttgtcat atttgagtgt atttaccagc atttaagcat
    14761 tagcaaagct gctgaatcac tatatatcac cccatcagcc gtgagccagt cgttacaacg
    14821 cttacgtgcg caatttaatg accctttatt cattcgctca ggaaaaggta tcgcgccgac
    14881 gacaacaggg ttgaatctgc atcaccatct tgagaaaaac ctcaaaggcc ttgaacaaac
    14941 aattaatata gtcaataagt ccgaactaaa gaaaaacttt attatctacg gccctcagct
    15001 catttcttgc tctaataata gtatgctgat ccgctgccta cggcaagatg cctctgtcga
    15061 gattgagtgc catgatatcc tcatgtcagc agagaatgct gaagaactgt tggttcaccg
    15121 caaagccgat ctggtgatca cccaaatgcc agtgatcagc cgttccgtca tttgcatgcc
    15181 attgcataca attcgcaata ctctaatttg tagtaataag catccgcgaa ttactgacaa
    15241 tagcacttat gaacaaatta tggcagaaga gttcacccaa ttaatagcca aatcagcagg
    15301 agttgatgat attcaaatgg aaatagacga gaagtttatg aatcgcaaaa tttcatttcg
    15361 tggttcctct ttgctgacaa taattaatag cattgcagtt acagatttac tgggaatagt
    15421 cccctatgaa ctttataact accaccgtga ctttcttaat ttaaaagaga taaaactaga
    15481 acatccgtta ccttcaataa agctttacat ttcctataat aaatcttctt taaataattt
    15541 ggtgttctcc cgatttattg accgtctaaa tgagagtttt tgatatataa ctatagacag
    15601 ctactataac tttcatctat ttattcacag cgcgaatgaa aaataaaaca ctctgcttta
    15661 cagtgtttac tttataatta acagtcttat ctcactgttc accctgtaac gttcagagag
    15721 cgtcttcaga tgtctattta taaaattcct cttccgctca atattctgga agctgcacga
    15781 gagcgtatta cctagacact caacacccta ccccgcgttt gtgtctcatt ttctggcggc
    15841 aaagactctg gtttaatgct gcatctgaca gccgaacttg cccgacaaat gggcaaaaaa
    15901 atctgcgttt tgtttatcaa ctgggaggc
//
LOCUS       DADLEE010000089        15546 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000089 DADLEE010000000
VERSION     DADLEE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.089"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            220..414
                     /gene="bfd"
                     /locus_tag="JQH51_003082"
     CDS             220..414
                     /gene="bfd"
                     /locus_tag="JQH51_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY6077336.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            487..963
                     /gene="bfr"
                     /locus_tag="JQH51_003083"
     CDS             487..963
                     /gene="bfr"
                     /locus_tag="JQH51_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY6077337.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(960..1427)
                     /locus_tag="JQH51_003084"
                     /pseudo
     CDS             complement(960..1427)
                     /locus_tag="JQH51_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1806..2117
                     /gene="rpsJ"
                     /locus_tag="JQH51_003085"
     CDS             1806..2117
                     /gene="rpsJ"
                     /locus_tag="JQH51_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY6077338.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2150..2779
                     /gene="rplC"
                     /locus_tag="JQH51_003086"
     CDS             2150..2779
                     /gene="rplC"
                     /locus_tag="JQH51_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY6077339.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2790..3395
                     /gene="rplD"
                     /locus_tag="JQH51_003087"
     CDS             2790..3395
                     /gene="rplD"
                     /locus_tag="JQH51_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY6077340.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3392..3694
                     /gene="rplW"
                     /locus_tag="JQH51_003088"
     CDS             3392..3694
                     /gene="rplW"
                     /locus_tag="JQH51_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY6077341.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3712..4533
                     /gene="rplB"
                     /locus_tag="JQH51_003089"
     CDS             3712..4533
                     /gene="rplB"
                     /locus_tag="JQH51_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY6077342.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4550..4828
                     /gene="rpsS"
                     /locus_tag="JQH51_003090"
     CDS             4550..4828
                     /gene="rpsS"
                     /locus_tag="JQH51_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY6077343.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4843..5175
                     /gene="rplV"
                     /locus_tag="JQH51_003091"
     CDS             4843..5175
                     /gene="rplV"
                     /locus_tag="JQH51_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY6077344.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5193..5894
                     /gene="rpsC"
                     /locus_tag="JQH51_003092"
     CDS             5193..5894
                     /gene="rpsC"
                     /locus_tag="JQH51_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY6077345.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5907..6317
                     /gene="rplP"
                     /locus_tag="JQH51_003093"
     CDS             5907..6317
                     /gene="rplP"
                     /locus_tag="JQH51_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY6077346.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6317..6508
                     /gene="rpmC"
                     /locus_tag="JQH51_003094"
     CDS             6317..6508
                     /gene="rpmC"
                     /locus_tag="JQH51_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY6077347.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6508..6762
                     /gene="rpsQ"
                     /locus_tag="JQH51_003095"
     CDS             6508..6762
                     /gene="rpsQ"
                     /locus_tag="JQH51_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY6077348.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6927..7298
                     /gene="rplN"
                     /locus_tag="JQH51_003096"
     CDS             6927..7298
                     /gene="rplN"
                     /locus_tag="JQH51_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY6077349.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7309..7623
                     /gene="rplX"
                     /locus_tag="JQH51_003097"
     CDS             7309..7623
                     /gene="rplX"
                     /locus_tag="JQH51_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY6077350.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7638..8177
                     /gene="rplE"
                     /locus_tag="JQH51_003098"
     CDS             7638..8177
                     /gene="rplE"
                     /locus_tag="JQH51_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY6077351.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8192..8497
                     /gene="rpsN"
                     /locus_tag="JQH51_003099"
     CDS             8192..8497
                     /gene="rpsN"
                     /locus_tag="JQH51_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY6077352.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8531..8923
                     /gene="rpsH"
                     /locus_tag="JQH51_003100"
     CDS             8531..8923
                     /gene="rpsH"
                     /locus_tag="JQH51_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY6077353.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8936..9469
                     /gene="rplF"
                     /locus_tag="JQH51_003101"
     CDS             8936..9469
                     /gene="rplF"
                     /locus_tag="JQH51_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY6077354.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9479..9832
                     /gene="rplR"
                     /locus_tag="JQH51_003102"
     CDS             9479..9832
                     /gene="rplR"
                     /locus_tag="JQH51_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY6077355.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9847..10350
                     /gene="rpsE"
                     /locus_tag="JQH51_003103"
     CDS             9847..10350
                     /gene="rpsE"
                     /locus_tag="JQH51_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY6077356.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10354..10533
                     /gene="rpmD"
                     /locus_tag="JQH51_003104"
     CDS             10354..10533
                     /gene="rpmD"
                     /locus_tag="JQH51_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY6077357.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10537..10971
                     /gene="rplO"
                     /locus_tag="JQH51_003105"
     CDS             10537..10971
                     /gene="rplO"
                     /locus_tag="JQH51_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY6077358.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10979..12310
                     /gene="secY"
                     /locus_tag="JQH51_003106"
     CDS             10979..12310
                     /gene="secY"
                     /locus_tag="JQH51_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY6077359.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12342..12458
                     /gene="rpmJ"
                     /locus_tag="JQH51_003107"
     CDS             12342..12458
                     /gene="rpmJ"
                     /locus_tag="JQH51_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY6077360.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12605..12961
                     /gene="rpsM"
                     /locus_tag="JQH51_003108"
     CDS             12605..12961
                     /gene="rpsM"
                     /locus_tag="JQH51_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY6077361.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12978..13367
                     /gene="rpsK"
                     /locus_tag="JQH51_003109"
     CDS             12978..13367
                     /gene="rpsK"
                     /locus_tag="JQH51_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY6077362.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13401..14021
                     /gene="rpsD"
                     /locus_tag="JQH51_003110"
     CDS             13401..14021
                     /gene="rpsD"
                     /locus_tag="JQH51_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY6077363.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14047..15036
                     /gene="rpoA"
                     /locus_tag="JQH51_003111"
     CDS             14047..15036
                     /gene="rpoA"
                     /locus_tag="JQH51_003111"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY6077364.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15077..15460
                     /gene="rplQ"
                     /locus_tag="JQH51_003112"
     CDS             15077..15460
                     /gene="rplQ"
                     /locus_tag="JQH51_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY6077365.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3647 c         4139 g         3753 t
ORIGIN      
        1 cgttggcgcg ggcgttgttg ctaaagttct gggctaatta cacgttaatt agttttgaat
       61 tgaaaagggc gcttcggcgc cctttttgct gcccgtaatt tgagctcgaa atatttagtc
      121 gtaattttgc tcgctgaact atggcgcaaa taaaactgtg ctattgtaat cataaccatt
      181 ctcatttaca ctttgtgcag aatttgaacg ggagcaatca tgtacgtttg tttgtgtaat
      241 ggtgtaagtg acaaaaaaat tcgccaggca gtccgtcagt ttcatccgca atctttccaa
      301 caattgcgta agtttattcc tgtgggaaat caatgcggta agtgcattcg cgccgcgcgc
      361 gaagtgatgc aggacgagtt gatgcagatg ccagaattta aagagattgc ctgaagccca
      421 cgcttatttt ttgacatcct gctagcccca tctacgctct aatagtggaa gcggagggac
      481 tataaaatga aaggtgatgt taaaatcata aattatctca ataaactatt gggaaatgag
      541 cttgtcgcaa tcaatcagta ttttctccat gcccggatgt ttaaaaactg gggcctgatg
      601 cgtctcaatg acgtcgaata ccatgaatcc atcgatgaga tgaaacacgc tgataaatat
      661 atcgagcgta ttttgttttt agaaggaatc cccaacttac aggatttagg caaactgggg
      721 attggtgaag atgtcgagga gatgctgcaa tctgacctgc gattagaact ggaaggcgcg
      781 aaggatctgc gtgaggctat cgcatatgcc gacagcgttc atgactatgt tagccgcgat
      841 atgatgatcg agatccttgc tgatgaagaa ggtcatatcg actggctcga aactgaactg
      901 gatctcatcg gtaaaatcgg cctacaaaat tacctgcaat cacaaattaa agttaaagat
      961 taacggccta tcagcaaact atcccagcct ataacgaaac ccgcagccgc cagaaatggc
     1021 ccaaaaggca gcgggttttt taatgattct tttcctctca tgagcagacc aataactacg
     1081 gctccgcaag cgaatgaggc agcaaggaaa accagtcgtg gtaagaaggc ccatgaatgc
     1141 taggcgccaa gggcggcgag aaacttcaca tcaccatagc ctaatccttc tttatgtcgc
     1201 agtatgcgat acccccagta aatgacagcg aatgtgccgt aaccaacaat tgccccccat
     1261 aacgcatcgg ctaaaccatc aggatgacaa acttgataga acaaaagacc tgaccagagt
     1321 aacgggcacg taaatctgtc aggtaacaag ccatgctttg catcccagaa aaaaagtagg
     1381 gctgataaac aagcgtacaa aattagaaag ggaagggtag tgtccataaa acatcctctg
     1441 taattggagt atcaccatcg aataactccg gatgctcagc aacagagaga gcgctgattt
     1501 gttaacctga ttccggtgaa aaacgctatc ttcacaacct tacaaatttc gtggaatgca
     1561 gctcgcaatc tgcgcgttgc gattaatttc tacccgatgc ttgcgtcagg ctcagatttg
     1621 cgtataatgc gcgggcttgt cgtagttgac agcaggttca atctaaacct cagttagccg
     1681 aatttggcta cctaacaatg ctcccaatcg gggagctacg taagaacggt tacactctcc
     1741 catcaatcgt aatgggtctg aggagtaatc attttcgttt ataaaataat tggagctctg
     1801 gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg
     1861 atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc
     1921 cgatcccgct gccgacacgc aaagagcgct tcactgttct gatctccccg cacgtcaaca
     1981 aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg tctggttgac atcgttgagc
     2041 caaccgagaa aaccgttgat gctctgatgc gtctggatct ggctgccggt gtagacgtgc
     2101 agatcagcct gggttaatca ggtcattgag cgattgagag gttgaaacaa tgattggttt
     2161 agtcggtaaa aaagtgggta tgacccgtat cttcacagaa gacggcgttt ctatcccagt
     2221 aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg
     2281 ctaccgtgct attcaggtga ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga
     2341 agctggccac ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg aattccgcct
     2401 ggctgaaggc gaagagttca ctgtaggtca gagcattagc gttgaactgt ttgctgacgt
     2461 taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg
     2521 ctggaacttc cgtacccagg acgctactca cggtaactcc ttgtctcacc gcgttccggg
     2581 ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca
     2641 gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg acgctgagcg
     2701 caacctgctg ctggttaaag gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa
     2761 accagctgtg aaggcgtaag gagatagcaa tggaattagt attgaaagac gcgcagagcg
     2821 cgctgactgt ttccgaaact accttcggtc gtgatttcaa cgaagcgctg gttcaccagg
     2881 ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg tgctcagaag actcgtgctg
     2941 aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg caccggccgt gcgcgttctg
     3001 gttctatcaa gagcccgatc tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg
     3061 accacagtca aaaagttaac aagaagatgt accgcggcgc gctgaaaagc atcctgtccg
     3121 aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa
     3181 ctaagctgct ggcacagaaa ctgaaagaca tggctctgga agatgtgctg atcatcaccg
     3241 gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct gcagaaggtt gacgtacgcg
     3301 atgcaactgg tatcgacccg gttagcctga tcgccttcga caaagtcgta atgactgctg
     3361 atgctgttaa gcaagttgag gagatgctgg catgattcgt gaagaacgtc tgctgaaggt
     3421 gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat
     3481 cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc aaagctgctg tgcagaaact
     3541 gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt aaagggaaag ttaaacgtca
     3601 cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg
     3661 ccagaatctg gacttcgttg gcggcgctga gtaagtcgga ggagtaatac aatggcagtt
     3721 gttaaatgta aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt taaccctgag
     3781 ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt
     3841 aacaacaatg gccgtatcac cactcgtcat atcggtggtg gccacaagca ggcttaccgt
     3901 attgttgact tcaaacgcaa caaagacggt atcccggcag ttgttgaacg tcttgagtac
     3961 gatccgaacc gttccgcgaa catcgcgctg gttctgtaca aagacggtga acgccgttac
     4021 atcctggccc ctaaaggcct gaaagctggc gaccagattc agtctggcgt tgatgctgca
     4081 atcaaaccag gtaacaccct gccgatgcgc aacatcccgg ttggttctac tgttcataac
     4141 gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac ttacgttcag
     4201 atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa
     4261 gtagaagcag actgccgtgc aactctgggc gaagttggca atgctgagca tatgctgcgc
     4321 gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc
     4381 gcgatgaacc cggtagacca cccacatggt ggtggtgaag gtcgtaactt tggtaagcac
     4441 ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact
     4501 gataaattca tcgtacgtcg ccgtagcaaa taattttaga ggataagcca tgccacgttc
     4561 tctcaagaaa ggtcctttta ttgacctgca cttgctgaag aaggtagaga aagcggtgga
     4621 aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat
     4681 gatcggtttg accatcgctg tccataatgg tcgtcagcac gttccggtat ttgtaaccga
     4741 cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc gcggccacgc
     4801 tgctgataaa aaagcgaaga agaaataagg taggaggaag agatggaaac tattgctaaa
     4861 catcgccatg ctcgttcttc tgctcagaag gttcgccttg ttgctgacct gattcgcggt
     4921 aagaaagtgt cgcaggctct ggatattttg acctacacca acaagaaagc ggctgtacag
     4981 gtcaagaaag ttctggaatc tgccattgct aacgctgaac acaacgatgg cgctgacatt
     5041 gacgatctga aagttacgaa aattttcgta gacgaaggcc cgagcatgaa gcgcattatg
     5101 ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat cactgtggtt
     5161 gtgtccgatc gctgagactc tggagactag caatgggtca gaaagtacat cctaatggta
     5221 ttcgcttggg tattgtaaaa ccatggaact ctacttggtt tgcgaacacc aaagaattcg
     5281 ctgacaacct ggacagcgat tttaaagtac gtcagtacct gactaaggaa ctggctaaag
     5341 cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag catccgtgta accattcaca
     5401 ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg
     5461 tcgtagcgga catcgctggc gttcctgcac agatcaacat cgccgaagtt cgtaagcctg
     5521 aactggacgc aaaactggtt gctgacagca tcacttctca gctggaacgt cgcgttatgt
     5581 tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct aaaggtatta
     5641 aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg
     5701 aaggtcgcgt accgctgcac actctgcgtg ctgacatcga ctacaacacc tctgaagcgc
     5761 acaccactta cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag atcctgggtg
     5821 gtatggctgc tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag cagcagcgta
     5881 aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa
     5941 tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc ggcagcttcg
     6001 gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg
     6061 ctatgacccg tgcagttaag cgtcaaggta agatctggat ccgtgtgttc ccggacaaac
     6121 cgatcactga aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt
     6181 gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc
     6241 tggcccgtga agcattcaag ctggcagcag cgaaactgcc gattaaaacc acctttgtaa
     6301 ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag aagagcattg aagagctgaa
     6361 caccgagctg ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg
     6421 ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac
     6481 tttactgaac gagaaggcgg gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc
     6541 gttgttagcg acaaaatgga gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac
     6601 ccgatctacg gaaaattcat caagcgtacg accaaactgc acgtacatga cgagaacaac
     6661 gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa
     6721 tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt aatacagtac actctctcga
     6781 tacgaataaa cggctcagaa atgagccgtt tattttttct acccatatcc ttgaagcggt
     6841 gttataatgc cgcgccctcg atatggggat ttttaacgac ctgattttcg ggtctcagta
     6901 gtagttgaca ttagcggagc actaaaatga tccaagaaca gactatgctg aacgtcgccg
     6961 acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg caccgtcgct
     7021 acgcaggcgt aggcgacatc atcaagatca ccatcaaaga agcaattccg cgtggtaagg
     7081 tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc
     7141 cggacggttc tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac aacaacagcg
     7201 agcagcctat cggtacgcgt atttttgggc cggtaactcg tgagcttcgt agtgagaagt
     7261 tcatgaaaat tatctctctg gcaccagaag tactctaagg agcgaatcat ggcagcgaaa
     7321 atccgtcgtg atgacgaagt tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa
     7381 gttaagaatg tcctgtcttc cggcaaggtc attattgaag gtatcaacct ggttaagaaa
     7441 catcagaagc cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct
     7501 attcaggttt ccaacgtagc aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc
     7561 tttagattcg aagacggcaa aaaagtccgt ttcttcaagt ctaacagcga aactatcaag
     7621 taatttggag tagtacgatg gcgaaactgc atgattacta caaagacgaa gtagttaaaa
     7681 aactcatgac tgagtttaac tacaattctg tcatgcaagt ccctcgggtc gagaagatca
     7741 ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa actgctggat aacgcagcag
     7801 cagacctggc agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg
     7861 caggcttcaa aatccgtcag ggctatccga tcggctgtaa agtaactctg cgtggcgaac
     7921 gcatgtggga gttctttgag cgcctgatca ctattgctgt acctcgtatc cgtgacttcc
     7981 gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc
     8041 agatcatctt cccagaaatc gactacgata aagtcgaccg cgttcgtggt ttggatatta
     8101 ccattaccac tactgcgaaa tctgacgaag aaggccgcgc tctgctggct gcctttgact
     8161 tcccgttccg caagtaaggt agggttacta aatggctaag caatcaatga aagcacgcga
     8221 agtaaaacgc gtagctttag ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat
     8281 catctctgat gtgaacgctt ccgacgaaga tcgttggaac gctgttctca agctgcagac
     8341 tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc
     8401 gcatggtttc ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg
     8461 cggtgaaatc ccgggtctga aaaaggctag ctggtaattg tcaccaattg aatcacggga
     8521 gtaaagacag atgagcatgc aagatccgat cgcggatatg ctgacccgta tccgtaacgg
     8581 tcaggccgcg aacaaagctg cggtcaccat gccttcctcc aagctgaaag tggcaatcgc
     8641 caacgtgctg aaggaagaag gttttattga agattttaaa gttgaaggcg acaccaagcc
     8701 tgaactggaa cttactctga agtatttcca gggcaaagct gttgtagaaa gcattcagcg
     8761 tgtcagccgc ccaggtctgc gcatctataa acgtaaagat gagctgccga aagttatggc
     8821 gggtctgggt atcgcagttg tttctacctc taaaggtgtt atgactgatc gtgcagcgcg
     8881 ccaggctggt cttggtggcg aaattatctg ctacgtagcc taatcggagg aaaaaatgtc
     8941 tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca
     9001 ggttattacg atcaaaggta aaaacggcga gctgactcgt actctcaacg atgctgttga
     9061 agttaaacat gcagataata ccctgacctt cggtccgcgt gatggttacg cagacggttg
     9121 ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg ttaccgaagg
     9181 cttcactaag aagctgcagc tggttggtgt aggttaccgt gcagcggtta aaggcaatgt
     9241 gattaacctg tctctgggtt tctctcatcc tgttgaccat cagctgcctg cgggtatcac
     9301 tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg
     9361 ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag ccttacaaag gcaagggggt
     9421 tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag gtaacactat
     9481 ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct
     9541 gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac atttacgcac aggtaattgc
     9601 accgaacggt tctgaagttc tggtagctgc ttctactgta gaaaaagcta tcgctgaaca
     9661 actgaagtac accggtaaca aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg
     9721 cgctctggaa aaaggcatca aagatgtatc ctttgaccgt tccgggttcc aatatcatgg
     9781 tcgtgtccag gcactggcag atgctgcccg tgaagctggc cttcagttct aaggtagagg
     9841 tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc aggaaaagct gatcgcggta
     9901 aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct ccttcacagc tctgactgta
     9961 gttggcgatg gtaacggtcg cgttggtttt ggttacggta aagcgcgtga agttccagca
    10021 gcgatccaga aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac
    10081 ggcactctac aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg
    10141 gcttccgaag gtaccggtat catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct
    10201 ggggttcata acgttctggc taaagcatat ggttccacca acccgatcaa cgtggttcgt
    10261 gcaactattg atggcctgga aaatatgaat tctccagaaa tggtcgctgc caagcgtggt
    10321 aaatccgttg aagaaattct ggggaaataa accatggcaa agactattaa aattactcaa
    10381 acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg cttgggtctg
    10441 cgtcgtattg gtcacaccgt agagcgcgag gatactcctg ctattcgcgg tatgatcaac
    10501 gcggtttcct tcatggttaa agttgaggag taagagatgc gtttaaatac tctgtctccg
    10561 gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt
    10621 aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt ctggcggtgg cgtacgtcgc
    10681 ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct
    10741 cgtaaagcag cgattacagc cgaagttcgt ctatctgacc tggctaaagt agaaggcggt
    10801 gttgtagacc tgaacacgct gaaagcggct aacattatcg gtatccagat cgagttcgcg
    10861 aaagtgatcc tggctggcga agtaacgact ccggtaactg ttcgtggcct gcgtgttact
    10921 aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata agtagcagat
    10981 ggctaaacaa ccgggattag attttcaaag tgccaaaggt ggcttaggcg agctgaaacg
    11041 cagactgctg tttgttatcg gtgcgctgat tgtgttccgt attggctctt ttattccgat
    11101 ccctggtatt gatgccgctg tacttgccaa actgcttgag caacagcgag gcaccatcat
    11161 tgagatgttt aacatgttct ctggtggtgc tctcagccgt gcttctatct ttgctctggg
    11221 gatcatgccg tatatttcgg cgtcgatcat tatccagctg ctgacggtgg ttcacccaac
    11281 gttggcagaa attaagaaag aaggggagtc tggtcgtcgt aagatcagcc agtacacccg
    11341 ttacggtact ctggtgctgg caatattcca gtcgatcggt attgctaccg gtctgccgaa
    11401 tatgcctggt atgcaaggcc tggtgattaa cccgggcttt gcattctact tcaccgctgt
    11461 tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg ggcgaacaga tcactgaacg
    11521 aggtatcggc aacggtattt caatcattat cttcgccggt attgtcgcgg gactcccgcc
    11581 agccattgcc catactatcg agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct
    11641 gttggttgca gtattagtat ttgcagtgac gttctttgtt gtgtttgttg agcgtggtca
    11701 acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca
    11761 gagcacacat ttaccgctga aagtgaatat ggcgggggta atcccggcaa tcttcgcttc
    11821 cagtattatt ctgttcccgg cgaccatcgc gtcatggttc gggggcggta ctggttggaa
    11881 ctggctgaca acaatttcgc tgtatttgca gcctgggcaa ccgctttatg tgttactcta
    11941 tgcgtctgca atcatcttct tctgtttctt ctacacggcg ttggttttca acccgcgtga
    12001 aacagcagat aacctgaaga agtccggtgc atttgtacca ggaattcgtc cgggagagca
    12061 aacggcgaag tatatcgata aagtaatgac ccgcctgacc ttggttggtg cgctgtatat
    12121 tacctttatc tgcctgatcc cggagttcat gcgtgatgca atgaaagtac cgttctactt
    12181 cggtgggacc tcactgctta tcgttgttgt cgtgattatg gactttatgg ctcaagtgca
    12241 aactctgatg atgtccagtc agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta
    12301 cggccgataa ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg
    12361 tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt
    12421 gcagtgccga gccgaagcat aaacagcgcc aaggctgatt ttttcgcata tttttcttgc
    12481 aaagttgggt tgagctggct agattagcca gccaatcttt tgtatgtctg tacgtttcca
    12541 tttgagtatc ctgaaaacgg gcttttcagc atggaacgta catattaaat agtaggagtg
    12601 catagtggcc cgtatagcag gcattaacat tcctgatcat aagcatgccg taatcgcatt
    12661 aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc atcctggctg cagcgggtat
    12721 cgctgaagat gttaagatca gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga
    12781 agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg
    12841 cctgatggat cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg
    12901 tcagcgtacc aagaccaacg cacgtacccg taagggtccg cgcaaaccga tcaagaaata
    12961 atcggggtga ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac
    13021 aagtctctga cggcgtggct catatccatg cttctttcaa caacaccatc gtgactatca
    13081 ctgatcgtca gggtaacgcg ttgggttggg caacagccgg tggttccggt ttccgtggtt
    13141 ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga
    13201 aagaatacgg catcaagaat ctggaagtta tggttaaagg tccgggtcca ggccgcgaat
    13261 ctactattcg tgctctgaac gccgcaggtt tccgcatcac taacattact gatgtgactc
    13321 cgatccctca taacggttgt cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc
    13381 aggtttgttg gagaaagaaa atggcaagat atttgggtcc taagctcaag ctgagccgtc
    13441 gtgagggcac cgacttattc cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa
    13501 ttgaacaagc tcctggccag cacggtgcgc gtaagccgcg tctgtctgac tatggtgtgc
    13561 agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt cagttccgta
    13621 actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc
    13681 tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact cgtgcagaag
    13741 cacgtcagct ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt
    13801 cttatcaggt tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc
    13861 gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa gccaacttgg ctggaagttg
    13921 atgctggcaa gatggaaggt acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg
    13981 acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta ccaaagagag
    14041 gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga tatcgagcaa
    14101 gtgagttcga cgcacgccaa ggtgaccctt gagcctttag agcgtggctt tggccatact
    14161 ctgggtaacg cactgcgccg tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag
    14221 gttgagattg atggtgtact acatgagtac agcaccaaag aaggcgttca ggaagatatc
    14281 ctggaaatcc tgctcaacct gaaagggctg gcggtgagag ttcagggcaa agatgaagtt
    14341 attcttacct tgaataaatc tggcattggc cctgtgactg cagccgatat cacccacgac
    14401 ggtgatgttg aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga tgagaacgcg
    14461 tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc ttctacccga
    14521 attcattcgg aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc
    14581 cctgtggagc gtattgccta caatgttgaa gcagcgcgtg tagaacagcg taccgacctg
    14641 gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt
    14701 cgtgcggcaa ccattctggc tgaacaactg gaagctttcg ttgacttacg tgatgtacgt
    14761 cagcctgaag tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg ccctgttgac
    14821 gatctggaat tgactgtccg ctctgctaac tgccttaaag cagaagctat ccactatatc
    14881 ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa
    14941 tctcttactg agattaaaga cgtgctggct tcccgtggac tgtctctggg catgcgcttg
    15001 gaaaactggc caccggcaag catcgctgac gagtaaccgg atcacaggtt aaggttttac
    15061 tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc gcaacagcag
    15121 ccatcgccag gctatgttcc gcaatatggc aggttcactg gttcgtcatg aaatcatcaa
    15181 gacgactctg cctaaagcga aagagctgcg ccgcgtagtt gagccgctga ttactcttgc
    15241 caagactgat agcgttgcta atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat
    15301 cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg gtggttacac
    15361 tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg ccgatggctt acatcgagct
    15421 ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa tgtaaaaaaa
    15481 cccgccccgg cgggtttttt tatacccgta gtatccccac ttatctacaa tagctgtact
    15541 cttttt
//
LOCUS       DADLEE010000090        15412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000090 DADLEE010000000
VERSION     DADLEE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.090"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1177)
                     /locus_tag="JQH51_003113"
     CDS             complement(<1..1177)
                     /locus_tag="JQH51_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY6077366.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="JQH51_003114"
     tRNA            complement(1376..1460)
                     /locus_tag="JQH51_003114"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="JQH51_003115"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="JQH51_003115"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY6077367.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="JQH51_003116"
     CDS             2804..4306
                     /locus_tag="JQH51_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY6077368.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="JQH51_003117"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="JQH51_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY6077369.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="JQH51_003118"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="JQH51_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY6077370.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="JQH51_003119"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="JQH51_003119"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY6077371.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="JQH51_003120"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="JQH51_003120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY6077372.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="JQH51_003121"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="JQH51_003121"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY6077373.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="JQH51_003122"
     CDS             complement(11871..13067)
                     /locus_tag="JQH51_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY6077374.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="JQH51_003123"
     CDS             13260..13763
                     /locus_tag="JQH51_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6077375.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="JQH51_003124"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="JQH51_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY6077376.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="JQH51_003125"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="JQH51_003125"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY6077377.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DADLEE010000091        15343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000091 DADLEE010000000
VERSION     DADLEE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15343
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.091"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQH51_003126"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQH51_003126"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY6077378.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQH51_003127"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQH51_003127"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY6077379.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQH51_003128"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQH51_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY6077380.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="JQH51_003129"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="JQH51_003129"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY6077381.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="JQH51_003130"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="JQH51_003130"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY6077382.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="JQH51_003131"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="JQH51_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY6077383.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="JQH51_003132"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="JQH51_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY6077384.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="JQH51_003133"
     CDS             complement(8064..9206)
                     /locus_tag="JQH51_003133"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY6077385.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="JQH51_003134"
     CDS             complement(9215..10228)
                     /locus_tag="JQH51_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY6077386.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQH51_003135"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQH51_003135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY6077387.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQH51_003136"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQH51_003136"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY6077388.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQH51_003137"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQH51_003137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY6077389.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="JQH51_003138"
     CDS             complement(12826..13950)
                     /locus_tag="JQH51_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY6077390.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQH51_003139"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQH51_003139"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY6077391.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4380 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaa
//
LOCUS       DADLEE010000092        14491 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000092 DADLEE010000000
VERSION     DADLEE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.092"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(113..379)
                     /locus_tag="JQH51_003140"
     CDS             complement(113..379)
                     /locus_tag="JQH51_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY6077392.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="JQH51_003141"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="JQH51_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY6077393.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="JQH51_003142"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="JQH51_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="JQH51_003143"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="JQH51_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY6077394.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="JQH51_003144"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="JQH51_003144"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY6077395.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JQH51_003145"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="JQH51_003145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY6077396.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JQH51_003146"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="JQH51_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY6077397.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="JQH51_003147"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="JQH51_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JQH51_003148"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="JQH51_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14107)
                     /locus_tag="JQH51_003149"
     CDS             complement(12641..14107)
                     /locus_tag="JQH51_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY6077398.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14104..>14491)
                     /locus_tag="JQH51_003150"
     CDS             complement(14104..>14491)
                     /locus_tag="JQH51_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6077399.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3862 a         3657 c         3419 g         3553 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt ttttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa cgcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgattccaca cgcgatactc attaaagtaa
    13861 atattcccgc ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag
    13921 agagcggaat aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt
    13981 ctttacctgc ggacagacca ccacctattt catttaggga tgacgataca atttgaatat
    14041 caagctgagc aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag
    14101 acatcatctc tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa
    14161 ttcctttgcg tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt
    14221 gccagtggca ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct
    14281 ttcaatgtga ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat
    14341 tcctggcttc attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact
    14401 gactggtact agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct
    14461 gcggtttgcg acaatgccat caatcggcgc a
//
LOCUS       DADLEE010000093        14468 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000093 DADLEE010000000
VERSION     DADLEE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.093"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(11..952)
                     /gene="fabY"
                     /locus_tag="JQH51_003151"
                     /gene_synonym="yiiD"
     CDS             complement(11..952)
                     /gene="fabY"
                     /locus_tag="JQH51_003151"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY6077400.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(997..1434)
                     /gene="dtd"
                     /locus_tag="JQH51_003152"
     CDS             complement(997..1434)
                     /gene="dtd"
                     /locus_tag="JQH51_003152"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY6077401.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1431..2303)
                     /locus_tag="JQH51_003153"
     CDS             complement(1431..2303)
                     /locus_tag="JQH51_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY6077402.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2297..2896)
                     /gene="yihX"
                     /locus_tag="JQH51_003154"
     CDS             complement(2297..2896)
                     /gene="yihX"
                     /locus_tag="JQH51_003154"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY6077403.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2995..3780)
                     /locus_tag="JQH51_003155"
     CDS             complement(2995..3780)
                     /locus_tag="JQH51_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6077404.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3814..4710)
                     /gene="yihV"
                     /locus_tag="JQH51_003156"
     CDS             complement(3814..4710)
                     /gene="yihV"
                     /locus_tag="JQH51_003156"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY6077405.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4878..5774
                     /gene="yihU"
                     /locus_tag="JQH51_003157"
     CDS             4878..5774
                     /gene="yihU"
                     /locus_tag="JQH51_003157"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY6077406.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5798..6676
                     /gene="yihT"
                     /locus_tag="JQH51_003158"
     CDS             5798..6676
                     /gene="yihT"
                     /locus_tag="JQH51_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY6077407.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6693..7934
                     /gene="yihS"
                     /locus_tag="JQH51_003159"
     CDS             6693..7934
                     /gene="yihS"
                     /locus_tag="JQH51_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY6077408.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8048..8974
                     /locus_tag="JQH51_003160"
     CDS             8048..8974
                     /locus_tag="JQH51_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY6077409.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9173..11209
                     /gene="yihQ"
                     /locus_tag="JQH51_003161"
     CDS             9173..11209
                     /gene="yihQ"
                     /locus_tag="JQH51_003161"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HAY6077410.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11255..12629
                     /locus_tag="JQH51_003162"
                     /pseudo
     CDS             11255..12629
                     /locus_tag="JQH51_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12672..14075
                     /locus_tag="JQH51_003163"
     CDS             12672..14075
                     /locus_tag="JQH51_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY6077411.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14143..>14468
                     /locus_tag="JQH51_003164"
     CDS             14143..>14468
                     /locus_tag="JQH51_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY6077412.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYL"
BASE COUNT         3492 a         3768 c         3647 g         3561 t
ORIGIN      
        1 tctccataag ctactcttct tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg
       61 gcagaacgat atacgtgcct tcaaacactg cacccggcgt ctcgtcgcca aagatttcga
      121 cctgcatctg cacccgtgct tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg
      181 caccaaggtc ggctaccgca tggggtttac cgctaatcgg tttgctgtag cggatatgcg
      241 catccgccag aataatcgtt ccgccgaggt ggcgttcacg cagcatcagc cagataagcc
      301 cccaaccggt gagcgtcgcc agcgagaata aactcccggc aaacagcgta tggtgcggat
      361 tctgattgcc ggtttccggc atggtggtga taaatttttg cccggtatat tgttgaatac
      421 gcacgcccat tttttcacta agcgggatat gttcgtacca cgcctgttgc agctgcgcgc
      481 accagtcgcc gcgatgcaaa atatcatcca gagtggcgac gggcttaatc atcaaaaaat
      541 ggcgaatcgg cgtggtggtt ggcgtggtga tttctccctg attaacaaac cccagcttgg
      601 cgaaaaactc caccgcgtct tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac
      661 gcgccaccga ctccagggtc atcgccatca gcgttcctaa ccctttgtcc tgcacgtccg
      721 ggtgaacgac cataaagcga atggacgctt cattgtcggc attaatatac aatcggccta
      781 ccgccaccag attaccctgc tcgtcgacga ccatctggtg atgcgccatc gcatcccacg
      841 cgtcgcgttc cgaacctttt ggttgatgca ggggcttacg caacatttcc cagcgaaagt
      901 gatagtaacg ctctaactct tcttctgttt gtggaacctg aaggtgatac atagcgatac
      961 tctctcttgt ttcccgtgac aaccctggaa gctggctcat acctgcaacc agaatgtcac
     1021 ggggccatca ttgaccagcg atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt
     1081 catctcttgc tggcggcagc gttcgacgaa atagtcatat aacgcctctg cgcgatccgg
     1141 tgatgcgcct ttggagaaac ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa
     1201 ctgggaaacc accagtacac tgccgcccgc ctgttgcacg ttgagattca tcttgccttc
     1261 ggcatcgcta aagatgcggt agccgagcac acgctcgcac agacggtttg ctttctgttc
     1321 gtcgtcatcc ttttcgacac ccaataacac caaaagtccc gcgccaattt cgcccgtcac
     1381 ttctccctcc acggtgacgc tggcacgggt tacgcgttga attaatgcaa tcatggttcg
     1441 tcgtcttctt cttgttcagc tgcttgtttt agtttgcggt attccccgag agtgacagta
     1501 atttccgcgc caagcaagac gatacaccat gtccagtaga cccaaacaaa gagaatgggg
     1561 atcaccgcca gcacaccgta aatgagctga tatgacggga acatggtgat ataaagcgcg
     1621 aaacctttct ttcctgcttc gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg
     1681 cggttaggta cgcggatggt aggaacaatg ctgtacagca accagaagga gatccacgac
     1741 aacagcaacg gaaaaatacg cagcacgttg tcgatgacag tattgagatc gctcgcccag
     1801 cgcagggaga gcaaatagga actgatcgcc agactggccc ctgccagcag cggccccagc
     1861 gttaaaatca tccagtacac ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc
     1921 cagatggtat tcaacgcgct atcgatggag tacatcaaca ataacgcggt gacgatcagc
     1981 ccgcacgccc caacggcggt catcttgttg gaattagcaa caaattgttc gatataccgc
     2041 tgaataacat cgccagtagc aggcagaaag ttggcaaaaa taaagtgacg taactgaatg
     2101 ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc
     2161 actaatgaga gcaacgacac ataggcaagg ttacctgcca gggttgtcat gttgtcctca
     2221 tcaatacgtt gccagagtag ttttagccag gcccatagtg gacgggtacg gtgcctggct
     2281 ttgtcctgaa tggtttttag cataacacct tcgcgaaata gtccgggatg gtggttttat
     2341 ctttcaccag aatactggta atgcctagct gattagctcc ttctatatta tcggcgttat
     2401 cgtcgaaaaa gaccgtatcg ctgggtgaaa aaccttccgc ctgcaaaaca tgctggtaaa
     2461 ttcgtgcttc aggtttgcgc atccccagat cttgcgacag atagatatgg tcagcagcat
     2521 cacgaatttc cgggtattct tccggccaga aggtggtatg caggcggttg gtattggaaa
     2581 gcaccaccac gcgatgcccc tgctcacgca gtttatgcat gatggcgatc acttccgggc
     2641 gcagcgcaac aaacaccgcc tgccagccgt gagagaactg ctcgtagctt agcggtagag
     2701 ccatctcatg acacagcgcc tctgcgaacg cttcgtcgct aatttcccca cgctcatgct
     2761 gatgaaacgc ctcccccata tgaaaactct tcttaagcga tgccagcgga atacgcgtta
     2821 aatcgctcca ggctcccagc acacggttaa agtcgatatc gacaatcaca ttacctaaat
     2881 caaagatata gagcattttt gcctcctttc gcgccacaag aaattaactg tagcgggaaa
     2941 ggatgggttt ggctatgtgg caaggtatga agccaggtta acggtcacaa aaatctacgc
     3001 gtcttcctgg ggaagaatga tttcaatttg gctgtcttgt aagcgtgatt ttagcgcagc
     3061 gggcggcagc tcgtcggtaa agagcgcagt gacctgtgcc acgttaccaa tttcaaccgc
     3121 cgccgaagca tgatacttag tgtgatcggc gaccagcaga atatttctcg cgtgcgccat
     3181 catcgttttc accacgttag cttcgtttac atcaaactcc atcaacgcgc catcgctctc
     3241 aatcgcccca acgcttgtta ccagataatc agcacgaaaa tcagccacaa aggacgccgc
     3301 tgaagggcca atgatcccgc tattatgaga gcgcaacgta ccgccgggca ccatcacttc
     3361 aaagcgcggg ttgtggtaaa gaatatgcgc cacacgcagg ctgttggtga ttatccgcaa
     3421 atgattatgg ttaagtaacg cccgggcaac atgctcaaca gtcgtaccaa tggtgataaa
     3481 tattgttgaa ccatcaggaa tatagtctgc caccgcttcg gcaatcgctt tttttttctc
     3541 ggtttgcgaa acctcacgct gctcgaacgc cgtattaacg acgctggaag cccgacccgc
     3601 gccaccgtga tggcgcgtaa tcaggccttg ctcgcttaat ttacgaatat cccggcggac
     3661 cgtctgcgtg gagacatcca gcagatttgc cagctcatca atattcatat acccacgctc
     3721 ggcgatcagc atgaggagtt ggtcgtgccg cgggttaccg gttagttcgg taaggctcat
     3781 gaaaattcct cgaaaaacca tcaccctggc attttataca aaaagtgaca aaaaagatcg
     3841 ggtttgatca cagtcaggga tcccggctcg tccaccggga cgtgtgcatt ttaacgccgc
     3901 tacaccgctg gcgaagcgga ctgactccgc taaatcccca cttgttgcca gcgccaccgc
     3961 caaagcgccg tgaaaaacat ctcccgcacc tgtggtatct accacatcaa ctttgaaggc
     4021 cggttgatgc tgacgcccac cattttccag ccagtcgcag cctgcgctac cctgggtcac
     4081 atagacatga ccatttgtga gcgtttgtgc ctgttttagc gcactggcca tctctttcac
     4141 gcccgttaag cgcgccagac ccggttctga aaaggccgcg tgatcgctta atgccaccag
     4201 ctcactgata tcctgcggcg taatgtcccc gtccagaacg gtcatcacac ccgcctgacg
     4261 ggccagggtg aaggcttttt tagcgccgtc gtgccagcgt acatctgcca gcacaacatc
     4321 ccactgagag aaatcaattt cctccaacca ctctgcgtca ggcagcagat ccgggctggg
     4381 gtagttaatg attatccgct cgcctttggt atccaccatg atggcggatt gcgaagattt
     4441 cgcctggtta taccgtttgg tgtaacgggt gttaacgccc caggattcca gctctgccag
     4501 caggctgttg cccgcgtcgt catcacctac gcgaccaata aaatcgacct gcgcccccag
     4561 ccttgccgcc gcaaccgctg ccgtcgccgc tggcccgcca ccaacttccg tataatttct
     4621 cgccacgtat ttaccgctct ccgtcggtaa cccttccacg taatagatgc gatccatcac
     4681 ggttatacct acacaagcaa cacgaatcat ggtcattcct taaacatttt gggatgcgtt
     4741 cattttaatt tcaccaattg tttaaaaagt gacggctgtc aattttttga cgataaatta
     4801 caaattcaat caaaaacaga caagcaaagc atgtcataaa tgacaaaaag tgacatcact
     4861 gtattcagga gagggttatg gcagcaatcg cgtttatcgg tttaggacaa atgggttcgc
     4921 caatggcgag caatttattg cagcaagggc atcaacttcg cgtctttgat gtgaacgccg
     4981 aagctgtgcg gcatctggta gacaaaggtg cgactcccgc cgccaacccg gcgcaggccg
     5041 ctaaagatgc cgaatttatc attaccatgt tgccgaatgg cgatctggtg cgcaacgtgt
     5101 tgttcggtga aaacggcgtt tgcgaaggct tatctaccga tgcgctggtc attgatatgt
     5161 ccaccatcca tccgctgcaa accgataaat tgattgccga tatgcaagcc aaaggcttca
     5221 gcatgatgga tgttccggta ggccgtactt ctgcaaatgc cattaccggt actctgttac
     5281 tgctggctgg cggcaccgct gaacaagttg aacgtgccac accgatcctg atggcgatgg
     5341 gcagtgagtt gatcaacgct ggcggtccgg gcatggggat ccgcgttaag ctcatcaaca
     5401 actacatgag catcgcgctc aatgcgcttt cggcagaagc cgccgttttg tgcgaagccc
     5461 tgaatcttcc cttcgatgtt gccgtcaaag tgatgagcgg taccgccgcc ggtaaaggtc
     5521 acttcaccac ttcctggcca aacaaagtcc tcagcggaga tctttctccc gccttcatga
     5581 tcgatcttgc ccataaggat cttggcatcg cccttgatgt cgccaaccag ctgcatgtgc
     5641 caatgccgct gggggccgcc tcacgggagg tttatagcca ggcgcgcgca gcgggtcgcg
     5701 gtcgccagga ctggtccgcc attctggaac aggtccgtgt cagtgccggg atgactgcca
     5761 aagtaaaaat gtaacgactg gataaaggaa taaatgaatg aataagtaca ccatcaacga
     5821 cattacgcgc gcatcgggcg gttttgccat gctggcggtc gatcagcgcg aagccatgcg
     5881 catgatgttt gccgcggctg gggcacccgc tccggtagcc gatagcgttt taactgattt
     5941 caaagttaac gctgcaaagg ccctctcgcc ttatgcctcg gcgattctgg tagatcaaca
     6001 attctgctac cgccaggtgg ttgagcaaaa cgcgattgcc aaaagttgcg ccatgattgt
     6061 cgccgccgat gagttcattc ctggcaacgg tattccggtc gatagcgtgg ttattgaccg
     6121 caaaatcaat ccgctacaga tcaaacaaga cggtggcaaa gccttaaaac tgctggtgct
     6181 gtggcgtagt gatgaagatg cgcagcaacg tctggatatg gtgaaagagt tcaacgaact
     6241 gtgccactca cacggtctgg taagcatcat tgagccagtc gtccgcccac cgcgtcgtgg
     6301 cgataaattc gatcgcgaac aagcgatcat cgatgccgcc aaagagctgg gcgacagtgg
     6361 cgctgacctc tacaaagttg aaatgccgct ttatggcaaa ggtccgcaac aagagcttct
     6421 ctgtgcttca caacgtctga atgaccatat caatatgcca tgggtgatcc tctcttccag
     6481 tgtcgacgaa aaactgttcc cgcgtgccgt acgcgtggca atgacggcgg gcgcatcggg
     6541 attcctggca ggccgtgcag tctgggcatc ggtcgtcggt ttaccagaca acgagctgat
     6601 gctccgtgac gtttgcgcac cgaaattaca acaacttggc gatatcgtcg acgaaatgat
     6661 ggctaaacgc cgctaagatg aggataaaag gaatgaaatg gtttaacacc ctaagccaca
     6721 accgttggct ggaacaggaa accgaccgca tctttgattt tggtaaaaat tccgtagtgc
     6781 cgactggttt tggctggtta ggcaataaag ggcaaatcaa agaagagatg ggcacccatc
     6841 tgtggatcac agctcgtatg ttgcacgttt attccgttgc tgcggcgatg ggtcgacctg
     6901 gcgcttacgc gttggttgat cacggtatta aagccatgaa cggcgcactg cgtgataaaa
     6961 aatatggcgg ctggtatgcc tgcgtgaatg acgagggcgt ggtggatgcc tccaaacagg
     7021 gctatcaaca cttctttgct ctgctgggtg ccgccagcgc cgtcacaacg ggtcacccgg
     7081 aagcgcgcaa gctgctcgat tacaccattg agattatcga gaaatatttc tggagcgaag
     7141 aagagcagat gtgcctggag tcctgggacg aagccttcag caaaaccgaa gagtaccgcg
     7201 gcggcaatgc caatatgcac gcggtggaag ctttcttgat tgtttatgac gtcactcacg
     7261 acaaaaaatg gctggatcgc gcgattcgcg tggcttccgt gattatccac gacgtcgcca
     7321 gaaataatca ttatcgcgtt aacgaacact tcgataccca gtggaatccg ctgccggatt
     7381 acaacaaaga taacccggcg catcgcttcc gcgcgttcgg aggtacacca ggccactgga
     7441 tcgaatgggg ccgtttaatg ctgcacatcc atgctgccct ggaagcccgt tgcgaacaac
     7501 caccagcatg gctgctagaa gatgccaaag gtctgtttaa cgccaccgtg cgcgatgcct
     7561 gggcacccga tggagcggac gggattgttt ataccgttga ctgggaagga aaaccggtgg
     7621 tccgcgaacg tgtacgttgg cctatcgtcg aagcaatggg tacggtctac gcgctctaca
     7681 ccgtcactgg cgatcgccag tatgaaacct ggtatcaaac atggtgggag tactgcatta
     7741 agtacctgat ggactatgaa aatggttcct ggtggcagga gctggatgcg gacaataagg
     7801 tcatcaccaa agtctgggac ggcaaacagg atatttatca cctgctgcat tgcctggtga
     7861 tcccgcgtat cccgttagcg cccggcatgg ctccagcggt tgcagcgggt ctgctggata
     7921 ttaatgcgaa ataactgatc tgaaaatagg gtgcgggttt tcccctcacc ctaaccctct
     7981 ccccaaaggg gcgaggggac cgtccggtgc agtttttggg tttgacatca gcctcacggg
     8041 aggctgtatg tcgttaatta aggtgccttg tatgcaaatc accaatatgc attgcagtgg
     8101 acagaccgtt tcacttgccg cgggggatta tcacgcgacc attgtgaccg tcggtgctgg
     8161 tctggctgaa ttaacctttc agggttgtca tttagtcatc ccgcacaaac ccgaagagat
     8221 gccgctggcg catctgggta aagtgcttat tccgtggccg aaccggatcg ccaacggctg
     8281 ttaccgttat caggggcagg aatatcagtt acccattaat gaacatagtt caaaagccgc
     8341 tattcacggg ctgcttgcct ggcgagactg gcaaattagt gaattaacca ccaccagcgt
     8401 taccctgaca gcttttctgc cgcccagtta cggctatccg tttatgctgg cttcacaagt
     8461 tgtttattcg ctcaacgcac acacgggtct ttccgttgag atcgcaagcc agaatatcgg
     8521 cactgtcgca gcgccttatg gcgtgggtat tcatccttat ttaacctgca acttaacatc
     8581 tgttgatgaa tatctgttcc agcttccggc aaaccaggtt tatgccgtcg atgaacacgt
     8641 aaatccgaca acgcttcacc acgttgatga gctggattta aatttcacgc aggcgaaaaa
     8701 aatagccgcg acaaaaatcg accacacctt taaaaccgct aatgatttgt gggaaatgac
     8761 cattacccat ccacagcagg cgctttcggt aagcctttgt tccgatcaac cctgggtgca
     8821 ggtttatagc ggtgaaaaat tacagcgtca gggactggca gttgaaccta tgagttgccc
     8881 gcctaacgcc tttaattcag gtatcgactt attattactg gaaccaggaa aaccgcaccg
     8941 cctgtttttt aatatttacg gacagagaaa ataacttgta ttgattagca gtcaaaatca
     9001 ttgaagaaca tttcactcgc taaaggcaac attatgtatc ttcaatttat taagttaaaa
     9061 acagctcagc ttctgtaaag tttttatata tcagttttat gagaatcatt ttacataagc
     9121 ctgtaataac aggaaggcaa caatctcgtt ctcaactcta tggagattag ttatggatac
     9181 gccacgtcca cagttattag attttcaatt tcatcagaat aacgacagtt ttaccctaca
     9241 ttttcaacaa cgtcttattt taacccataa caaagataat ccttgtttat ggattggctc
     9301 aggtatagcg gatatcgata tgttccgcgg taatttcagc attaaagata aactacagga
     9361 gaaaattgcg cttaccgacg ccatcgtcag ccagtcaccg gatggttggt taattcattt
     9421 cagccgtggt tctgacatta gcgccacgct gaatatctct gccgacgatc agggccgttt
     9481 attgctggaa ctacaaaacg acaaccttaa ccacaaccgt atctggctgc gccttgccgc
     9541 tcaaccagag gaccatatct acggctgcgg cgaacagttt tcctacttcg atctgcgcgg
     9601 caaaccgttc ccgctatgga ccagtgaaca aggcgttggt cgcaacaaac aaacctatgt
     9661 cacctggcag gccgactgca aagaaaatgc gggcggcgac tattactgga ctttcttccc
     9721 acagcctacg tttgtcagca cgcagaagta ttactgccat gttgataaca gttgctacat
     9781 gaacttcgac tttagcgccc cggaatacca tgaactggcg ctgtgggaag acaaagctac
     9841 gctacgtttt gaatgtgctg acacatacat ctccctgctg gaaaaattaa ctgccctgct
     9901 gggacgccag ccagaactgc ccgactggat ttatgacggg gtaacgctcg gcattcaggg
     9961 cgggacggaa gtgtgccaga agaaactgga caccatgcgt aacgcgggcg tgaaggttaa
    10021 cggcatctgg gcgcaggact ggtctgggat ccgtataacc tcttttggca agcgcgtgat
    10081 gtggaactgg aagtggaaca gcgaaaacta cccgcaactg gattcacgca ttaagcagtg
    10141 gtatcaggag ggcgtgcagt tcctggccta tatcaacccg tatgttgcca gcgataaaga
    10201 tctctgcgaa gaagcggcac aacacggcta tctagcaaaa gatgcctctg gcggtgacta
    10261 tctggtggag tttggcgagt tttacggcgg cgttgtcgat ctcactaatc cagaagccta
    10321 cgcctggttc aaggaagtga tcaaaaagaa catgattgaa ctcggctgcg gcggctggat
    10381 ggctgacttc ggcgagtatc tgcccaccga cacgtacctg cataacggcg tcagtgccga
    10441 aattatgcat aacgcctggc ctgcgctgtg ggcgaagtgt aactacgaag cccttgaaga
    10501 aacgggcaag ctcggcgaga tcctcttctt tatgcgcgcc ggttctaccg gtagccagaa
    10561 atactccacc atgatgtggg cgggcgacca gaacgtggac tggagtctcg acgatggcct
    10621 ggcgtccgta gtcccggcgg cgctgtcgct ggcaatgacc ggacatggtc tgcatcacag
    10681 cgacattggc ggttacacca ccctgtttga gatgaagcgc agcaaagagc tgctgctgcg
    10741 ctggtgcgat ttcagcgcct tcacgccgat gatgcgcacc cacgaaggta accgtcctgg
    10801 cgacaactgg cagtttgacg gcgatgcgga aactatcgcc cattttgccc gtatgactac
    10861 cgttttcacc accctgaaac cttatctgaa agaggccgtc gcgctgaatg cgaagtccgg
    10921 tctgccggtc atgcgcccgc tgttcctgca ttacgaagac gatgcacata cctattccct
    10981 gaaatatcag tacctgttag gccgcgacat tctggtcgct ccggtacacg aagaaggtcg
    11041 cagcgactgg acgctctatc tgccggagga taactgggta catgcctgga cgggtgaagc
    11101 gttccggggc ggggaagtta ccgttaatgc gcccatcggc aagccgccgg tcttttatcg
    11161 cgccgatagc gaatgggcgg cactgttcgc gtcgttaaac agcatctaat cacatccgcc
    11221 cgtggggaaa ctcatgggca aagggagaac atcgatgagt cacatcacaa cggaagatcc
    11281 agcaacttta cgcctgccct ttaaagagaa actctcttac ggtattggcg acctggcctc
    11341 taacatcctg ctggatatcg gtacgcttta tcttttgaag ttttataccg acgttctggg
    11401 gctgccaggc acctatggcg gcattatctt tttgatttca aaattcttta ctgcgtttac
    11461 cgatatgggt accggcatta tgttggattc ccgacgcaag atcggtccaa aaggtaagtt
    11521 ccgtcctttt attctgtatg cgtcattccc ggtcacctta ctggcgatcg ccaactttgt
    11581 cggcacaccg tttgatgtca ccggtaaaac ggtgatggcc actattctgt ttatgcttta
    11641 cggactgttt ttcagcatga tgaactgctc ctacggcgca atggtcccgg caatcaccaa
    11701 aaaccccaac gaacgcgcct cactggcggc atggcgtcag ggcggcgcta cgctgggcct
    11761 gctgctgtgc acggtgggat tcgtgccagt tatgaatctt atcgaaggta atcagaaact
    11821 tggctatatc ttcgccgcca cgctgttttc actgttcggc ctgctgttta tgtggatctg
    11881 ctactcaggc gtgaaagagc gttatgtcga aacccaacca accaatccgg cgcaaaagcc
    11941 tggcctgttg caatctttcc gcgcaattgc cggtaaccgc ccactgttca ttctgtgtat
    12001 tgccaacctc tgcaccttag gggcgtttaa cgtcaagctc gccattcagg tctattacac
    12061 ccagtacgta cttaacgatc ccatcctgtt gtcgtatatg ggatttttca gcatgggctg
    12121 tattttcatc ggcgtgttcc tgatgcctgg cgcagtcaga cgttttggta agaagaaggt
    12181 ctatatcggc ggcctgctga tttgggtgct gggcgatctg ctcaactatt tcttcggcgg
    12241 cggctcggtc agctttgtgg cgttctcctg cctggcgttc ttcggctcag cgcctgaact
    12301 gggcgctggt ttccgacacc gtcgagtacg gcgagtggcg caccggcgtg cgttcggaag
    12361 gcacggtcta caccggcttc accttctttc gcaaagtgtc tcaggcgctg gctggtttct
    12421 tccccggctg gatgctgacg caaattggct atgtgccgaa cgtcgcacag gctgaccaca
    12481 ccattgaagg gttacgccag ttgatcttca tctacccaag cgcactggcg gtagtcacca
    12541 ttgtggcgat gggttgcttc tacagcctga acgagaagat gtatgtccgc attgtggaag
    12601 agatagaagc ccgtaaacgc acggcgtaat aataataaac gacgccctgc ggggcgttat
    12661 aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg cgggaaaaaa
    12721 tcgcctatgg tatgggcgac gtcggttcga atttaatgct ctgcatcggt actctgtatc
    12781 tcctcaaact ttataccgat gaattaggga tgcctgctta ctatggcggg attatctttc
    12841 tggtcgcgaa gttttttacc gcgtttaccg atatgctcac cggattttta ctcgactcgc
    12901 ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat tttatatgca gcagttccgg
    12961 cggcgttaat tgcaacgctc cagtttatcg ccaccacctt ttgtttgccg gttaaaacga
    13021 cgattgccac cacgctgttt atgatgtttg gactttcata cagtctgatg aactgctcgt
    13081 atggtgcgat gatcccggca attaccaaaa acccgaatga gcgcgcgcag ctcgcggctt
    13141 accgccaggg tggtgcaacc atagggctat tgatttgtac cgtggcgttt attccgttgc
    13201 agtcgctttt ttctgactca accgtcggtt atgcctgtgc ggcacttatg ttctccattg
    13261 gcggctttat ttttatgatg ctgtgctaca gaggcgtcaa agagcattat gtggacacag
    13321 cgccaaccgg acataaagcc agtattctca aatctttttg cgcgatattt cgtaatccac
    13381 cattgctggt tttatgcatt gctaacctgt gtactctggc ggcatttaat atcaaactgg
    13441 cgattcaggt ctattacacc cagtatgtgc tgaatgatat taatttattg tcgtggatgg
    13501 gattcttcag tatgggatgc atcctcgtcg gcgtattact ggtgccagta actgtaaaat
    13561 gttttggtaa aaaacaggtt tatttagctg gcatggtgct atgggcggtg ggtgatatac
    13621 tgaattattt ctggggaagt aactctttca ctttcgtcat gttctcttgt gtcgcctttt
    13681 ttggcacggc ctttgtaaac agcctgaact gggcactggt accagatacc gtagattacg
    13741 gtgaatggaa aaccggtatt cgtgccgaag gttctgttta taccggttat accttctttc
    13801 gtaaaatttc tgccgcactt gctggcttct tgccaggcat tatgctgacg caaattggtt
    13861 atgttcccaa catcgcgcaa agcgatgcga cattacaggg tttgcgtcag ctcattttta
    13921 tctggccttg tgcactggca attattgctg cattgacgat gggattcttt tacacactca
    13981 atgaaaaacg gtttgcattg attattgagg aaatcaacca acgcaaaaat aaagagatgg
    14041 cgacagaaaa aaaaacggct tccgtaacgt tataaatgtc aatagccgct atttccatcc
    14101 tggtgggtgg cggcctccct acgtttaaaa aatggactta ttatgaaaaa gattaatgca
    14161 ataattttat tatcttctct gacctctgcc tcggtatttg ctggcgcata cgtcgaaaac
    14221 agggaggcat ataatcttgc ctccgatcag ggcgaggtta tgctacgtgt gggttataac
    14281 ttcgatatgg gcgcgggtat tatgttaacc aatacctaca cctttcagcg agaagatgaa
    14341 ctaaaacatg gctataatga aattgaaggt tggtatcctt tattcaaacc aaccgataaa
    14401 ttaaccatcc agcccggtgg cttaattaat gataagagta tcagttcggg tggtgcagtg
    14461 tatctgga
//
LOCUS       DADLEE010000094        14421 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000094 DADLEE010000000
VERSION     DADLEE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.094"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..132)
                     /locus_tag="JQH51_003165"
     CDS             complement(<1..132)
                     /locus_tag="JQH51_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY6077413.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="JQH51_003166"
     CDS             315..458
                     /locus_tag="JQH51_003166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077414.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /locus_tag="JQH51_003167"
     CDS             complement(677..2215)
                     /locus_tag="JQH51_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY6077415.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="JQH51_003168"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="JQH51_003168"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY6077416.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="JQH51_003169"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="JQH51_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY6077417.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="JQH51_003170"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="JQH51_003170"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY6077418.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="JQH51_003171"
     CDS             complement(7051..7659)
                     /locus_tag="JQH51_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY6077419.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="JQH51_003172"
     CDS             complement(7730..8815)
                     /locus_tag="JQH51_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6077420.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="JQH51_003173"
     CDS             complement(8818..9180)
                     /locus_tag="JQH51_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY6077421.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /locus_tag="JQH51_003174"
     CDS             9717..11630
                     /locus_tag="JQH51_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY6077422.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="JQH51_003175"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="JQH51_003175"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY6077423.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="JQH51_003176"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="JQH51_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY6077424.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="JQH51_003177"
     CDS             complement(13472..13681)
                     /locus_tag="JQH51_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077425.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="JQH51_003178"
     CDS             13965..14327
                     /locus_tag="JQH51_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY6077426.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3524 a         3841 c         3624 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaacag
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac c
//
LOCUS       DADLEE010000095        14316 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000095 DADLEE010000000
VERSION     DADLEE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.095"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..3051
                     /locus_tag="JQH51_003179"
                     /pseudo
     CDS             1..3051
                     /locus_tag="JQH51_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="JQH51_003180"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="JQH51_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="JQH51_003181"
     CDS             6768..7175
                     /locus_tag="JQH51_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077427.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="JQH51_003182"
     CDS             7717..8805
                     /locus_tag="JQH51_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY6077428.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="JQH51_003183"
     CDS             8816..11095
                     /locus_tag="JQH51_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077429.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="JQH51_003184"
     CDS             11088..12224
                     /locus_tag="JQH51_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY6077430.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="JQH51_003185"
     CDS             12221..14224
                     /locus_tag="JQH51_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY6077431.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3546 a         3754 c         3655 g         3361 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcac
//
LOCUS       DADLEE010000096        14155 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000096 DADLEE010000000
VERSION     DADLEE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14155
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.096"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..460
                     /locus_tag="JQH51_003186"
                     /pseudo
     CDS             1..460
                     /locus_tag="JQH51_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            625..4743
                     /gene="pic"
                     /locus_tag="JQH51_003187"
     CDS             625..4743
                     /gene="pic"
                     /locus_tag="JQH51_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HAY6077432.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4894..5136)
                     /locus_tag="JQH51_003188"
                     /pseudo
     CDS             complement(4894..5136)
                     /locus_tag="JQH51_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5175..6125)
                     /locus_tag="JQH51_003189"
                     /pseudo
     CDS             complement(5175..6125)
                     /locus_tag="JQH51_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6176..6751)
                     /locus_tag="JQH51_003190"
                     /pseudo
     CDS             complement(6176..6751)
                     /locus_tag="JQH51_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6755..7155
                     /locus_tag="JQH51_003191"
                     /pseudo
     CDS             6755..7155
                     /locus_tag="JQH51_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7558..8139)
                     /locus_tag="JQH51_003192"
     CDS             complement(7558..8139)
                     /locus_tag="JQH51_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077433.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8186..12043)
                     /gene="sigA"
                     /locus_tag="JQH51_003193"
     CDS             complement(8186..12043)
                     /gene="sigA"
                     /locus_tag="JQH51_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HAY6077434.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12404..13441)
                     /locus_tag="JQH51_003194"
     CDS             complement(12404..13441)
                     /locus_tag="JQH51_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY6077435.1"
                     /translation="MHRGFCVAHEPSILLPSVGAQLSNPDNITDNADRISDEWKAFDR
                     AIESNHGVPEAAARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSV
                     DGKVSDADLTYLEEVANSTGQEVDKSRLTSQACARTALITDVGIALATELETAGQKWS
                     LGFPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLV
                     AQNLISRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNR
                     QFAAIGTEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNT
                     YGAVAQFQFTF"
     gene            13393..13770
                     /locus_tag="JQH51_003195"
                     /pseudo
     CDS             13393..13770
                     /locus_tag="JQH51_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13809..>14155)
                     /locus_tag="JQH51_003196"
     CDS             complement(13809..>14155)
                     /locus_tag="JQH51_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY6077436.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3547 a         3325 c         3472 g         3811 t
ORIGIN      
        1 gggcttcgtc tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tgtggtgacg
       61 agtctcatcg tctatggaaa cgacattcgt gctggatgca ctggagcagg cgttgtgggc
      121 ccgtcgtccg tctggcacca tccatcacag cgataaaggc tctcagtatg tgtcactggc
      181 ctataaggcg cgactaaaag aagcaaaact gctggcatca acagggagta ctggtgactc
      241 gtatgacaac gcgatggcag agatcatcaa gggcctttac aaagcggagg taatacaccg
      301 taagagctgg aaaaaccgga cagaagtgga gctggccaca ctcacgtggg tggactggta
      361 taacaatcga cgattgctgg aaaggctggg ccatatccca ccggcagaag cagaaaaagc
      421 ttattatgct tccatcggaa atgatgatct ggcagcctga gttcacagat aaaacactct
      481 ccaggaaacc cggggcggtt cagttcacaa aaacacatta atgcagtaac tatattttcc
      541 tttctggtga taacgtcggg ttatcattag cttcttcagc tattttactt ttatatccct
      601 tgtaaacatc atggagaatc catagtgaat aaagtttatt ctcttaaata ttgccccgtc
      661 accggggggc ttattgctgt ctctgaactt gcccgcaggg taataaaaaa gacatgccga
      721 agattaacgc atattcttct ggctggcatt ccagcaatct gtctgtgtta ctctcagata
      781 tcccaggcgg gtattgtccg ttccgatatt gcctatcaga tttatcgtga tttcgccgaa
      841 aacaaagggc tttttgtacc tggtgccaat gatattccgg tatatgataa ggacggaaaa
      901 cttgtgggaa gactgggtaa agccccaatg gccgatttca gcagtgtgag ctcaaatggc
      961 gttgctacgc ttgtatcgcc tcagtatatc gtcagcgtaa agcataacgg aggatatcgg
     1021 agtgtgagct ttggtaatgg gaaaaataca tattcccttg ttgaccgtaa taaccaccct
     1081 tctattgact tccatgctcc acgtctgaat aaactggtta cagaagttat tccctcagcg
     1141 gtaacatcag aaggaaccaa agccaatgct tataaataca ctgaacgtta caccgctttt
     1201 tatcgggtgg gtagtggtac gcagtacact aaggacaagg acggaaattt agttaaggtt
     1261 gccggtggat atgcttttaa aacaggagga accacaggag ttcctctgat atctgatgca
     1321 acaatagtct ctaatcccgg gcaaacttat aatcctgtaa acggcccttt acctgactat
     1381 ggagcccctg gggatagtgg ttctcctttg tttgcttatg ataaacaaca aaaaaaatgg
     1441 gttattgttg ctgtattaag agcatatgca ggtattaatg gtgctacgaa ctggtggaat
     1501 gtcataccaa cagattatct gaaccaggtt atgcaggacg atttcgatgc ccccgtagac
     1561 tttgtttccg gactgggccc cctgaactgg acatacgaca aaacatcagg cacaggtacc
     1621 ctgagccagg gcagtaaaaa ctggaccatg cacgggcaga aagacaatga cctcaatgcc
     1681 ggtaaaaatc tggtattcag cgggcagaat ggtgcaatta tcctgaaaga cagtgtgact
     1741 cagggtgccg gttatctcga atttaaagac agttacaccg tatctgctga atccggaaaa
     1801 acatggacgg gtgccggcat tattactgac aaggggacga atgtaacctg gaaggtcaac
     1861 ggcgttgccg gtgacaactt gcataagctg ggggaaggaa ccctgaccat aaacggaaca
     1921 ggtgtaaacc cgggaggact gaaaacggga gacggtatcg ttgtacttaa ccagcaggca
     1981 gacactgcag gtaatatcca ggccttcagt tcagtgaacc tcgccagcgg acgtccgacc
     2041 gtggtgctcg gggatgcccg tcaggtcaat ccggataaca tttcatgggg ataccgggga
     2101 ggtaagcttg accttaatgg taatgccgtt accttcaccc gactgcaggc tgctgattac
     2161 ggggcggtga ttacaaataa tgcacagcaa aaatcccagc ttttactgga tcttaaggct
     2221 caggatacaa atgtcagtga accgacgatt ggaaatatat ccccctttgg tggtaccggc
     2281 actccaggaa acctgtacag catgatactc aacagccaga cccgcttcta tattctgaaa
     2341 tctgccagct atggtaacac tctgtggggg aacagcctga atgatccggc tcagtgggag
     2401 tttgttggca tgaacaaaaa caaagcagtt cagacagtaa aagataggat cctggccggg
     2461 cgggcaaaac aacccgttat ctttcatggt cagctgaccg ggaatatgga tgtcgccatt
     2521 ccacaggtgc cggggggaag aaaggtaatc tttgatggta gcgtgaacct gccggaaggt
     2581 accctgagtc aggacagtgg caccctgata ttccagggac atccggttat ccatgcctcc
     2641 atcagtggca gtgcaccggt cagcctgaac cagaaagact gggaaaaccg tcagtttaca
     2701 atgaaaacac tgtcgctgaa agacgctgac ttccatcttt cacgtaacgc ctcgctgaac
     2761 agtgacatta agtcggataa cagccatatc acactgggaa gtgacagggc atttgtggat
     2821 aaaaatgacg gaacaggaaa ttatgtcatt ccggaggaag gtacctctgt cccggacacc
     2881 gtgaatgaca ggagccagta tgaagggaat attacgctga accataactc agccctggat
     2941 atcggcagca ggttcaccgg ggggattgac gcttatgaca gtgccgtcag catcacctct
     3001 ccggacgtcc tgttgacagc cccgggtgct tttgccggca gttcactgac agtgcatgat
     3061 ggcggtcatc ttacagcact gaacggtctt ttcagcgacg ggcatattca ggccggtaag
     3121 aacggcaaaa tcaccctgag cggtacaccg gttaaagata cggctaatca gtatgcccct
     3181 gctgtatatc tgacggacgg atatgacctg accggcgata acgcagcact ggaaattacc
     3241 cgtggagcac atgcttccgg tgatattcat gcctctgcgg catcaacagt taccatcggg
     3301 tctgacacgc cggcagaact ggcttctgcg gaaacggctg catcggcgtt tgccggcagt
     3361 cttcttgagg gctataacgc agcattcaat ggtgccataa ccggtggcag ggctgatgtc
     3421 agtatgcata atgcactgtg gactctgggt ggggactctg ccatccacag tcttaccgtc
     3481 agaaacagcc gtattagttc tgaaggagac cgtacattcc gtaccctgac ggtgaataaa
     3541 ctggatgcaa caggcagtga ttttgttttg cgtacggacc tgaaaaatgc cgataaaatt
     3601 aatgtgactg aaaaagccac tggttcagat aacagcctga acgtcagctt tatgaataat
     3661 cctgctcagg gacaggccct gaatattcct ctggtcacgg caccggcggg aacttcagca
     3721 gagatgttta aggccggcac ccgggtgaca ggtttcagtc gggtgacccc aaccctgcat
     3781 gttgatacca gtggtggcaa tacgaagtgg atactggatg gttttaaagc ggaggctgat
     3841 aaagccgctg ccgcgaaggc tgacagtttc atgaatgccg ggtataaaaa cttcatgacg
     3901 gaagttaaca atctgaacaa acgtatgggt gacctgcgtg acacaaacgg tgatgccggt
     3961 gcctgggcgc gcatcatgag tggtgccggt tctgcagacg gtggttacag tgataattac
     4021 acccatgttc aggtcggctt tgacaaaaaa catgaactgg acggtgtgga cctgtttacc
     4081 ggtgtcacga tgacctatac cgacagcagt gcagacagcc atgcattcag cggaaagacg
     4141 aaatcggtgg ggggcggtct gtatgcttca gcattgtttg agtccggtgc ctatatcgat
     4201 ttgattggta aatatattca ccatgacaat gattacacag gtaactttgc tagcctggga
     4261 acgaaacact acaacaccca ttcctggtat gccggtgctg aaacgggtta ccgctatcac
     4321 ctgacagagg acacgttcat tgagccgcag gctgaactgg tttacggcgc cgtgtccggg
     4381 aaaacattcc gctggaaaga cggtgatatg gacctgagca tgaagaacag ggacttcagt
     4441 ccgctggttg gaagaacagg ggttgaactg ggcaagacct tcagtggtaa ggactggagt
     4501 gtgacggccc gtgccggaac cagctggcag tttgacctgc tgaataatgg agataccgta
     4561 ctgcgtgatg cgtccgggga gaaacggata aaaggagaga aggacagccg gatgctgttt
     4621 aatgttggta tgaatgcgca gataaaggac aatatgcgct ttggtctgga gtttgagaag
     4681 tcagcctttg gtaaatataa cgtggataat gcggtaaacg cgaatttccg gtatatgttc
     4741 tgatgtgggg gtaaagggct gccggaaggc agcctttctc agagactctt aaaatataaa
     4801 actgaaggtc ttcggtaagg gggagtgtat tagacgtcaa ctagttttgc cgactggcgc
     4861 gagcctgttt aagattctgt gtaaatgcct ttctcagaag tgaccgtcca ggcggtcacc
     4921 gaactcgata ataaagcggc tcattgccat tcgccagtcc ttcaacggca tcgtccattt
     4981 ctgggacgca gactggattg ccagccacac cacttttttc accgagtcgt ctgtcgggaa
     5041 cactttacgc tttttgatcg catggcggat cacgctgttt agcgactcga tggcattcgt
     5101 cgtatagatc actttgcgga tgtccgttgg ataagcggta agcgtacagc gagggccgta
     5161 ttgacgggga tgtgttattc agctggcagt gctatgcgcc acggaagcag ttcgctgacc
     5221 cggttgaccg gccagtctgc tatgacgcca agcacatggc gaaggtagct ttctggatcc
     5281 acgtcattca gtttgcacgt cccgatcagg ctgtacagta gcgcgccccg ctcaccacca
     5341 tggtcagagc cgaagaacag gaagttttta cgacccagac tgaccgcccg cagggcattt
     5401 tcagcgatgt tgttgtcgat ttccacccag ccattactgc agtacacgtt cagggccttc
     5461 cactgtttca gtaggtatgc gaacgctttt gccgtatccg agtgacgcga cagtgttttc
     5521 atctgttgct gtatccggtc atacagtgac tgcatcagtg gcgcggctct tgcttttctt
     5581 gccgccagac gctgttctgc tgaacatccc cggacttctg cctcgatggt atacagttca
     5641 ccgatacgct gcagggcttc cgtggtgatg tcggttggca ctcttgcatg cacatcgtgg
     5701 atttttctcc gggcatgggc catacacgca gcttccgtta ttctgccgga ttcgtataac
     5761 gcccggtaac caccgtaagc atctgcctga agcacaccgc tgtaaccggc taggtgattc
     5821 tgtggatgga tacctttccg gtccgggctg tacgcgaacc agaccgccgg gggcatctgt
     5881 gaaccggcgt tacggtcatc acggacatag acccacagcc gggcagttct tgttttacca
     5941 ctgcccggct cctggaccgg aacggggata tcatccgcat gaactttacc gggcatcagt
     6001 acatactggc gcaggacgtc atacagcggc tccagcagtt cagcaacggc accggtccag
     6061 cgacccagtg tggcacggct cagctccacg ccctgacgac ggtatatttc tgactggcgg
     6121 tataactgtc aacgacggat gaaaagtggc tcttccgcaa cctgggtgga gattttcatg
     6181 ccagtggact cattaccctc tgcctgagta gttcaaatcc tgctcgacca tacatctggc
     6241 gtttcagcat cttcttcagg cgatttacat gaccttcgac aacaccatta ctccagcgac
     6301 tactgattgc ctcacatata gctgcagcat cagcttccat ccccgccgcc acgcgccgaa
     6361 attctgctga accgctttcg tggactcgag tgaaccaact gcttagctgt gatttatttt
     6421 gggttttcag aatacggtag aactcgagtg ccagttgctg cgctattttc agctcaggtt
     6481 ctttttcaca catcagacta ataaatcggg aagcataatt ttcttcatcc ctggttattc
     6541 tccagggcat caaccagcgg cttacccggg agactgaagg aagtctgacg gcagtagtaa
     6601 ccggtgggtt ccagccttta cgccatttgg caaccgcatc cctgacgatg gtttcactgc
     6661 ctgtaaatcc ccgggccacc atttcccgcc atatccggct ggcgttataa ttaccacttt
     6721 ctcgttgctc ttttaaccac tccctccatg gtgtcagcga aaaacgaaac cgctgttgaa
     6781 atccctggaa agctggttgc ggaaaagatg aagacccagt cgcgacactc agagttggcg
     6841 aaggcgttcg cgtacgcact taaccagtgg ccggcactga cgtactatgc gaacgatggc
     6901 tgggtggaaa tcgacaacaa catcgctgaa aatgccttgc gggcggtcag tctgggtcgt
     6961 aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc ggggagcgct actgtacagc
     7021 ctgatcggga cgtgcaaact gaatgacgtg gatccagaaa gctaccttcg ccatgtgctt
     7081 ggcgtcatag cagactggcc ggtcaaccgg gtcagcgaac tgcttccgtg gcgcatagca
     7141 ctgccagctg aataacacat ccccgtcaat acggccctcg ctgtacgctt actcatcacc
     7201 tgctattaat ccccgagtca gtacttcgcg gcctgacgcg atcaccctac tcagtacttc
     7261 agagcccgcc tccggtacct gcctggcgat cgcccctgtg caacaaaata aatcaaccgt
     7321 tatcaaacca cgatcgttcg tatgcgccgc cgatgacgcg cgcctgaaaa acgcgtcaat
     7381 atgagttccc cggaagctta cgataatacg cttatgaaca ttacaacacg ctcatgcgga
     7441 acctggatgg gggaaggaac ggtgaagctg ttagcgaatg accggcgttt atacaaacgc
     7501 cggtcatgat gaatgatatt tcagaatata cctcgggcta aaataccggg acagagatca
     7561 ggcgtgatta atctgcagca tttttaataa cagaagtact gagcttactt tacctttgat
     7621 gatgtaatag ttatttccgt cggtactcat ttttccctga aataatgtca cggatgtttc
     7681 ttcctgctga tcctgcaggc gcagtcgtaa ggtcatattt ttaatgagct gagatccggg
     7741 tggcaagggg tatttgctgt cagatactgg gcgactgagt ctgatgtggc gcgtatacag
     7801 cccgttttcg tacagggtgg cctctgttat ggtgtcagtg accagcatgt gggagacaat
     7861 gctgtattct tttttaaagc acccaccaga aacggtgatt gtaatgataa tggtcttctc
     7921 gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg agaactcagt
     7981 tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt ctttttttat
     8041 tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa agctaataat
     8101 gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctccgg tagaagaagg
     8161 gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg ttgatcgcgt
     8221 tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag cgaagattat
     8281 cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt tcacctttga
     8341 tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac aggtcaaact
     8401 ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag gatttaccca
     8461 catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg gtcacgctca
     8521 ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc agttctgcct
     8581 gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca gcacccacat
     8641 acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc gagtactcat
     8701 tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca aatatagcgg
     8761 aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta ctgctggcat
     8821 gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc agctcatgtt
     8881 ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacca gaagctgaac
     8941 ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca cgcagatccc
     9001 ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta ccggacatat
     9061 acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg gtcagggtcc
     9121 acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg ctgaaaccta
     9181 tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct gttatcaggt
     9241 caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac agactgttat
     9301 tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca gactggctca
     9361 gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact gtcacagtat
     9421 ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt tttaatgatt
     9481 gcagggctga ctgtccgttt actctccata aggtgttata catgctggct gaggctgcag
     9541 gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct gtagccagcg
     9601 gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca aacagaatag
     9661 atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga gcctttaacg
     9721 tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg ctgtatttat
     9781 tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata gctttatcta
     9841 catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg tctgtattaa
     9901 ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt attttgctgt
     9961 tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg gagcgaagct
    10021 gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg tcaatgtatg
    10081 cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct tttacattag
    10141 catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa ttaaatttac
    10201 gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta tattccgtat
    10261 tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag tctttttgac
    10321 ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt tctgtcgcat
    10381 gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata tcgactgagc
    10441 catcaaagat cagattggca ttattgttat gttgctgggt agtattgata atatgattta
    10501 tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta gtaagtgata
    10561 atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt gctgcgattt
    10621 tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg gtaaaataga
    10681 tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc gtacctttac
    10741 ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca gtcccattac
    10801 ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta cctattttat
    10861 gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta ttatcaatat
    10921 ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc tggttcttat
    10981 cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat acccctcctc
    11041 ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt ttaccatcca
    11101 gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta ttaaataacg
    11161 agtagtttgt ccaggctata ttggtactac tgataaaacc aactgtactg gtaacaccat
    11221 ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca ctatcgccaa
    11281 aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg tttttccagt
    11341 ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca aacagatatt
    11401 gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca attatttgtt
    11461 ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa agtgagaagt
    11521 cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga tgaaccgaaa
    11581 agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat gactgtgttg
    11641 ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca cctccaattg
    11701 atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt tctggaaaat
    11761 gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt ctgaactctc
    11821 ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata tccagcattg
    11881 ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata ccgaggataa
    11941 ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca gcaactaatc
    12001 cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa ctccgatatt
    12061 tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag tcatggtacg
    12121 taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca gatgtaacaa
    12181 aatgaaaaaa aagataagca caagtgattg attggttaga tattgatcac ttggtgtttg
    12241 tattttgaat tttgcgatca aaatttgatc tttgttgttt ccggtcaatc ctttacaggt
    12301 tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt gatgatggaa
    12361 acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag gtgaactgga
    12421 actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca tcaaggtgaa
    12481 ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg ttaccggcca
    12541 gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat tctgtgccaa
    12601 tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc agatccacat
    12661 caaatgcagt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc tgcgggcgga
    12721 tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg gagatcaggt
    12781 tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca ccgatgtcgg
    12841 cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg ctgtcataat
    12901 ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg gggaagccca
    12961 gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc acatcagtga
    13021 tcagtgccgt gcgggcacag gcctgtgatg tcaggcggct tttgtccacc tcctgcccgg
    13081 tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta ccgtcgacac
    13141 tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg ggaagggccg
    13201 ccacagcaga aacgcccagc tgtgcggcgg catgtgtgtt acggaagtcc cggaggtgtt
    13261 ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg cggtcgaagg
    13321 ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga ttggagagct
    13381 gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat ccacgatgta
    13441 tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg tgaactgtac
    13501 gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt caggcaaatg
    13561 cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga acacacgtta
    13621 tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg ggctgcgttg
    13681 tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga aagagcgctt
    13741 cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt gtctgactgc
    13801 ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag tgcaatatcc
    13861 cacataaatc cagtcaactc acgagccacc gccgtaatgg ctacttgtga ttttttccct
    13921 tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca tgcaatatct
    13981 ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc aacttttgca
    14041 ggtgtacgat aagaccaggc ggcttcgtaa agcagacgtc tcagttcgct gtttccaact
    14101 tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg cacca
//
LOCUS       DADLEE010000097        14114 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000097 DADLEE010000000
VERSION     DADLEE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.097"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            650..1441
                     /locus_tag="JQH51_003197"
     CDS             650..1441
                     /locus_tag="JQH51_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077437.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1827..2033
                     /locus_tag="JQH51_003198"
     CDS             1827..2033
                     /locus_tag="JQH51_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY6077438.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2119..2691
                     /locus_tag="JQH51_003199"
     CDS             2119..2691
                     /locus_tag="JQH51_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077439.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2693..2977)
                     /locus_tag="JQH51_003200"
     CDS             complement(2693..2977)
                     /locus_tag="JQH51_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077440.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2948..3091
                     /locus_tag="JQH51_003201"
                     /pseudo
     CDS             2948..3091
                     /locus_tag="JQH51_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4260..5279
                     /locus_tag="JQH51_003202"
     CDS             4260..5279
                     /locus_tag="JQH51_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6077441.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5389..6261
                     /locus_tag="JQH51_003203"
     CDS             5389..6261
                     /locus_tag="JQH51_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY6077442.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6589..9717
                     /gene="ag43"
                     /locus_tag="JQH51_003204"
     CDS             6589..9717
                     /gene="ag43"
                     /locus_tag="JQH51_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAY6077443.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLA
                     DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGT
                     LAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNT
                     TLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGN
                     KLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     SGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKA
                     SSDNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFG
                     HGSAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSS
                     RGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAE
                     GYNSQATLNVTF"
     gene            9838..12354
                     /locus_tag="JQH51_003205"
     CDS             9838..12354
                     /locus_tag="JQH51_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077444.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12430..12885
                     /locus_tag="JQH51_003206"
     CDS             12430..12885
                     /locus_tag="JQH51_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077445.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            12986..13117
                     /locus_tag="JQH51_003207"
     CDS             12986..13117
                     /locus_tag="JQH51_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY6077446.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            13296..14114
                     /locus_tag="JQH51_003208"
     CDS             13296..14114
                     /locus_tag="JQH51_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY6077447.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         3825 a         3483 c         3474 g         3332 t
ORIGIN      
        1 actcttgact ataaagatga tgacgggatc agaacagtta ttaatccata caacaaaata
       61 cagaaaacaa cagaaaaaaa aaaaaaaaaa aaaataacag acattactta acaaagaaca
      121 ttacagtcag tgtgtacatt acagatttat attcaggtgt tgcgggacta cattacagca
      181 gagaggggta aggacagagt tacaagtact actagagaga atatcactgc acagatactt
      241 ctatgagtat atctttatga gagatccgtc tacaggattg ggttataagg tgtatttaca
      301 aggtataatc acaggatcat ttacatgacc atacttctgt aaataatact aattaacctt
      361 aattattttt ttatggtaca tgaactacct gtagctgtaa cgataaacaa atcacccaaa
      421 atgaaaaata ctggttatca cagttcagag aactcgtatg agtgtccccc tgagcaatgc
      481 ttcattttct gtgattctgt gtgatagagt tatactgagt acagtggcag taacactgct
      541 ttccatcact tgaggataaa attcattaaa aaattaattg catgaattaa caggtttttt
      601 tatgaaatgt tatgcatctc aaccaatatg attaacagaa aacttcatca tgacaacacg
      661 tataacgttc agtatgacca gtgatgagac gctgcgaatt gtggatgagt actgtcatac
      721 ccacaaactt tcgcgctcta aggtaatcaa cgcattactc agcgccacag cacctgtcct
      781 taatgatatt aactgttatt atcaacttgc aggcgaactg cagtcacgct tattaaatgg
      841 agtgtatcaa cgggatcttc ctcgcaaacg caatgtcgtc agtgcagaga agtattgtct
      901 ggaaatctgg gaaaacaaac ttttcaccag aagaatcctg gagtttgaca gttcaaatgg
      961 tgttctgtac gcgctgaaac ataaaagaca ctatcgccgg gataagatga ttggaagggt
     1021 tgaaagtcgg tgtatcaaag acatttgcga ataccagatg cagctatccg gggagaagac
     1081 gaaatatgcc tgttttattt atatcgaaag aacaatatac aatcacgata atccatctga
     1141 taaaatacct gtaaaagcgg ctgttggtaa tgcagtcatc ctgctggcaa aggatgtgat
     1201 atataacgag tacttttttg atttgcggca gtcatttttt gtgagcgtta cagatctgat
     1261 ggtctctggc gcaaaaggta ttccggagac acagacatat ccggacgttt attgctggat
     1321 ccctcttttt tccataaatt ccggcgtgct cattacccct gtatataaaa ttgaccctct
     1381 gaaacccgta acggtaaaaa aaccggataa gatcactgtt gtttgtaatt acagagaata
     1441 atttcagcct tctaccttca ccgcgacagt gtttacctct gctttatctt ccgttcagta
     1501 aatacaaacc accgctctgg caggtacagg gcgtggttta tctccgatac tcccctgtgc
     1561 agtaataatc atcactgaaa atactgatcc cgtcacggca aaacgccatc ccatattccg
     1621 gcaacacaaa atcatcttcc gtttataccg gattcactga caaaacagac aacctgtatt
     1681 gcctgtacac aacagtttct ctcctggcag gaatattccc ctactcattt tttacgtttc
     1741 tccccggttg ttatgccggt gaaggtggtg cgtcgttttc atcaccaacg ccggttgaga
     1801 ttaacaacat ccggaggaac attctcatga ataccccagt ttcactgatg gatgaccaga
     1861 tggtcgacat gtcgtttatc actcagctga ccggcctgac cgataagtgg ttttataaac
     1921 tcatcaagca tggagccttt ccgcccccca tcaaactggg ccgtagctcc cgctggctga
     1981 aaagcgaagt ggaagcctgg ctgcaggcgc gtattgcaca gtcccgtccg taattcctga
     2041 ccgttatccg ttccaccgca gcaaaggcat gatccggccc ccggaggcat tttgctgcgc
     2101 gctaaaggag agaatattat gcaacaactt gaaaacaggt gtgacctgct gctcatccag
     2161 catcagaaat ggatgacaag cgtgacgagg cttatcgtcg ctcatggaat ggggtctccg
     2221 catcttcatg gttatcaccg tctgaccctg gcacactttt ttctgccgga aaaaggctcc
     2281 gtcatttcgg tggcccccca gggactctat caggtcgtca acccgggaac accaccgttc
     2341 attcctgcca ttcaggaagg gttaatgacc agcattcaga cccatgaaat catgctgctt
     2401 acacatttta atctgggcgg cgttttactc agtgagctac atcgtctggg tgaaaaccgg
     2461 ctggcaaaca ggctgaacag cctgctccgg cgtttcgacg atcgtgatct ctaccacacg
     2521 ctgatatggc tgtgctggta tgatctgatg tgtgcccact ccatgcagcc ctggactgaa
     2581 gagttaaagc ataaatcaca tgcagagctt gaaaactggg ctgtggcgcg caaacgggaa
     2641 aagcgcgagc tggaactgat gattgacgag tacctgttat acgcctgctg attcataacg
     2701 ttcccggggt tccttcctca tcgttacaca cagccggacg gctatctatg gctgtccgcc
     2761 aaatgaccat cgccgcattt cgttccgctg aaaacggtac gtagtcagtg ataccggaat
     2821 ttccggagag ggccgcaggt atattgcttc tgtgcaattg atgcttattt acattggatg
     2881 tagttatttc tgcatccaat gctatcggaa agagattaac cagcaaatgg ttcctcaaat
     2941 cgtattgata tttacccggc gtattgagca ccaccatctg acactccgtt cccgcatcaa
     3001 acgactggac cgaaaaacga tttacttttc acgtgctgtg gaaattcacg aaaaaattat
     3061 tgggtcattc atcgaaaaac atatgttctg atgactggag tcatcctcca gaaatatacg
     3121 tcaactgaac aatatcgatt ttttatggga ttctatacac ttttccatat ccctctgaaa
     3181 atcagtcatc agctcaccaa cacctcttct gaaacttgta ttaaacttat tgcttaaata
     3241 gatgatataa catctcttcc attgcactga atcggcgaaa actgccgttt caataatctc
     3301 aacaatatta tttatcggct aactttatca gtaaacaatg gaatgtcaca ctgatgaaat
     3361 gaacattcag agtaagatga taaaattcat ataaacgcca ttgctgaata ttatttattt
     3421 catacagata aacgataaaa tacaaaaggg tatatttctc aatgtaacag tcaattattc
     3481 atctccactg gcgagcacgg tcagcacaca aacaaagtta gtcctcctgc ttaatcattg
     3541 cgtatcagaa actaaaaaag aaaaaattct gcagacaact taacaatctt catcacattt
     3601 cctgaagaca gacatatcca caaacggtag tcagcgttga attaacaagt cttacagagc
     3661 tatacgtagc ttataaagcc actcaatgtt catcatcacc cgactactta tctggaatac
     3721 caccagtata aactcatcaa taaaaacacc tgttgttaaa ataagaatac tgtcagcagt
     3781 aaattattaa aacaacatct gtccacacta tctgttcttt taacgccaac ggcagcgaaa
     3841 atagtgcatc acagccttct tttttctttc gccaccactc agacaaacac aacactcaca
     3901 cccacaccac aaaacagaat caaaacataa aattaacaat aacagtcaaa aggaaaacac
     3961 agaatattga ggtggactgt caccaggaca gcgcatacga cgcatgtgat cagcacatcc
     4021 cgttcttcgc tggcatcccc aaaatgcaga acaaacaatc ataacattat gaatataaaa
     4081 gaaaatatga tttttcacca tgaaaaaaac atcaatgtat ctctgtataa aaataatgat
     4141 aacatgacat aaacatttaa aaacataaat aaacagcaac aaaaacattt gataatcact
     4201 tagtgattaa atacaattaa gacgattttg ttataagaga ggcaccatgg agtgagataa
     4261 tgaataacga acagatgctt atcaaatgca gagatccccg taaatgtcgg ttttatgccg
     4321 aatatctgaa tcacaaatac cctaatgaat tccctcactt tgcgcctgac aacccacaaa
     4381 attgtatcag gaatgttaat ttatttttaa aggaaaagta ttgtaatgtt tcacctcccc
     4441 cctttggtac ttttggtact actgaagaag aagcactcaa taacgaaatt aatgagcttg
     4501 aaattattgc cgggcaggca atactgcctg aagagagttt tctctggttg aaaactgacg
     4561 aacttgcgac tttctttacc tggactacta tttatctgtc gaaggaagac catacaaaat
     4621 tcccgaaaat aaaccataat tcatcaggaa taaaaaacgg aattgaagtc aggaaaatac
     4681 gaacagttac cgatgaaacg ttaaacactt acaaaaaaag taaccttccg gataatccat
     4741 catcccataa cgaacgaata aaagtaatca tttcatattt tgactcctgc cattctttaa
     4801 ttaataaaaa tgcaaaaagc atatacctcg aggaactcag gcaggcgtgg cttaaaaaga
     4861 aaagcgcttg tcaaaagttt aagtggctcc ttcctgacga tgatgaaatg tgtcagtggt
     4921 tctggacaag actgaaacaa aaacaggatg aaaaacatcc agtacataac ccagtgaccc
     4981 ggtggttcat accatcttca acgaaagaat attatcttgc ctctcttgtc gcctttgctt
     5041 tatggaaaga agcgcctgat accattgaac tgttcagaac acgaataaat catgcctggc
     5101 atcagaaaaa acaaaaggac aaatatcgga gtgatggtaa aaaggccatt aatatacata
     5161 taagaagcaa tgtgaaagaa atgcttgatg agctcagaac tgtttacggt ctgagaacag
     5221 gagagttttt tgaaatgctg attacagaga aatatcttga acacaagcga aggaagtaat
     5281 tcattacctg ttacattcta aataatctca gacagatatt atctccgtga atcccccgcc
     5341 acctttccgg tgcgcggggt tttgtctttt ttcaccggga atacatgtat gaatccgtct
     5401 gatgccattg aggcaattga aaaaccgctc tcctccctgc cttactcact ttcccgtcac
     5461 atcctggaac atctgcgcaa actcaccagt cacgaacccg tgattggcat tatgggtaaa
     5521 agcggggccg gtaaatcttc actctgtaat gcactgtttc agggggaggt cacaccggtc
     5581 agtgatgttc acgccggcac ccgggaagtg cagcgcttcc gtctgagtgg tcacggtcac
     5641 agcatggtta tcactgacct gcccggggtg ggcgagagcc gggacaggga tgcagagtat
     5701 gaagccctgt accgtgacat tctgcctgaa ctggacctgg tgctgtggct gattaaagcc
     5761 gatgaccggg ccctgtctgt ggatgagtat ttctggcgac acatcctgca gtgcggacat
     5821 cagcaggtgc tgtttgtggt gacgcaggct gacaaaacgg aaccctgcca tgaatgggat
     5881 atggcaggta ttcagccttc tcccgcacag gaacagaaca ttcgtgagaa aacggaggcg
     5941 gtattccgtc tgttccggcc cgtacatccg gttgttgccg tatcggcccg caccggctgg
     6001 gaactggata cgctggtcag tgcgctcatg acagcgcttc ccgaccatgc cgccagcccc
     6061 ctgatgaccc gactgcagga cgagctgcgc acggagtctg tccgctctca ggcccgtgaa
     6121 cagtttaccg gtgcggtgga ccggatattt gacacagcgg agagcgtctg tgttgcctct
     6181 gttgtccgta cggccctgcg cgctgttcgt gacaccgtgg tctctgttgc ccgcgcggta
     6241 tggaactgga tcttcttctg aacctgttgt ggatgatgtc ctccctgcct ctgagtctgc
     6301 tcacaaaagc gctgttttcg ttactgtctc tcttgtccgt gcaatagact gataatagaa
     6361 taaaacgatc gatacctatt ttaccgatcg ttaatatcga tcgttatgct aataataact
     6421 cctgttagca acgtgcgcag atacacacag acatgagatt cagggaacaa cagagccaca
     6481 cgtcagaaac ttccgtcagc tggacctccg gcactgtaac cctttacctg ccggtatcca
     6541 catctgtgga taccggcttt tttattcacc ctcactctga ttaaggaaat gctgatgaaa
     6601 cgacatctga atacctgcta caggctggta tggaatcaca ttacgggcgc tttcgtggtt
     6661 gcctccgaac tggcccgcgc acagggtaaa cgtggcggtg tggcggttgc actgtctctt
     6721 gccgcggtca cgtcactccc ggtgctggct gctgacatcg ttgtgcaccc gggtgaaaca
     6781 gtgaatggcg gaacactggt aaaccatgac aaccagtttg tatccggaac agctgatggc
     6841 gtgactgtca gtaccgggct tgagctgggg ccggacagtg acgaaaacac cggcgggcaa
     6901 tggataaaag cgggtggcac aggcagaaac accactgtca ccgcaaatgg tcgtcagatt
     6961 gtgcaggcag gaggaactgc cagtgatacg gttattcgtg atggcggagg gcagagcctt
     7021 aacggactgg cggtgaacac cacgctggat aacagaggtg agcagtgggt acacggggga
     7081 gggaaagctg ccggtacaat tattaaccag gatggttacc agaccataaa acatggcgga
     7141 ctggcaaccg gaaccatcgt caacaccggt gcagaaggtg gtccggagtc tgaaaatgtg
     7201 tccagcggtc agatggtcgg agggacggct gaatccacca ccatcaataa aaatggccgg
     7261 caggttatct ggtcttcggg gatggcacgg gacaccctca tttacgccgg tggtgaccag
     7321 acggtacacg gagaggcaca taacacccga ctggaggggg gtaaccagta tgtacacaac
     7381 ggtggcacgg caacagagac gctgataaac cgtgatggct ggcaggtgat taaggaagga
     7441 ggaactgccg cgcataccac catcaaccag aaaggaaagc tgcaggtgaa tgccggcggt
     7501 aaagcgtctg atgtcaccca gaacacgggc ggtgcactgg ttaccagtac agctgcaacc
     7561 gtcaccggca caaaccgcct gggagcattc tctgttgtgg agggtaaagc tgataatgtc
     7621 gtactggaaa atggcggacg cctggatgtg ctgaccggac acacagccac taatacccgc
     7681 gtggatgatg gcggaacgct ggatgtccgc aacggtggca ccgccaccac cgtatccatg
     7741 ggaaatggcg gtgtactgct ggccgattcc ggtgccgctg tcagtggtac ccggagcgac
     7801 ggaaaggcat tcagtatcgg aggcggtcag gcggatgccc tgatgctgga aaaaggcagt
     7861 tcattcacgc tgaacgccgg tgatacggcc acggatacca cggtaaatgg cggactgttc
     7921 accgccaggg gcggcacact ggcgggcacc accacgctga ataacggcgc catacttacc
     7981 ctttccggga agacggtgaa caacgatacc ctgaccatcc gtgaaggcga tgcactcctg
     8041 cagggaggcg ctctcaccgg taacggcagc gtggaaaaat caggaagtgg cacactcact
     8101 gtcagcaaca ccacactcac ccagaaagcc gtcaacctga atgaaggcac gctgacgctg
     8161 aacgacagta ccgtcaccac ggatgtcatt gctcagcgcg gtacagccct gaagctgacc
     8221 ggcagcactg tgctgaacgg tgccattgac cccacgaatg tcactctcgc ctctggtgcc
     8281 acctggaata ttcccgataa cgccacggtg cagtcggtgg tggatgacct cagccatgcc
     8341 ggacagattc atttcacctc cacccgcaca gggaagttcg taccggcaac cctgaaagtg
     8401 aaaaacctga acggacagaa tggcaccatc agcctgcgtg tacgcccgga tatggcacag
     8461 aacaatgctg acagactggt cattgacggt ggcagggcaa ccggaaaaac catcctgaac
     8521 ctggtgaacg ccggcaacag tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg
     8581 gtggaagcca ttaacggtgc caccacggag gaaggggcct ttatccaggg gaataagctg
     8641 caggccggtg cctttaacta ctccctcaac cgggacagtg atgagagctg gtatctgcgc
     8701 agtgaaaatg cttatcgtgc agaagtcccc ctgtatgcct ccatgctgac acaggcaatg
     8761 gactatgacc ggattctggc aggctcccgc agccatcaga ccggtgtaag cggtgaaaat
     8821 aacagcgtcc gtctcagcat tcagggcggt catctcggtc acgataacaa cggcggtatt
     8881 gcccgtggag ccacgccgga aagcagcggc agctatggct tcgtccgtct ggagggtgac
     8941 ctgctcagaa cagaggttgc cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc
     9001 cattcttccg ttgatgttaa ggatgatgac ggctcccgtg ccggcacggt ccgggatgat
     9061 gccggcagcc tgggcggata cctgaatctg atacacaacg cctccggcct gtgggctgac
     9121 attgtggccc agggaacccg ccacagcatg aaagcctcat cggacaataa cgacttccgc
     9181 gtccggggct ggggctggct gggttcgctg gaaaccggtc tgcccttcag tatcactgac
     9241 aatctgatgc tggagccgca actgcagtac acctggcagg gactctccct ggatgacggc
     9301 caggataacg ccagttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggc
     9361 ttccgtctgg gcagccacca cgatatgaac tttggtaaag gcacctcatc ccgtgacacc
     9421 ctgcgcggca gtgccaaaca cagtgtgcgt gaactgccgg tgaactggtg ggtacagcct
     9481 tctgttatcc gcaccttcag ctcccggggg gacatgagca tgggtacagc cgcagccggc
     9541 agtaacatga cgttctcacc gtcacagaat ggtacgtcac tggacctgca ggccggactg
     9601 gaagcccgtg tccgggaaaa tatcaccctg ggcgttcagg ccagttatgc ccacagcatc
     9661 aacggcagca gcgctgaagg ttataacagt caggccacac tgaatgtaac cttctgataa
     9721 ttctgcattg cctctctgtg gtcccggtca tgatgaccgg gacccggacg ggggaaaacg
     9781 tttcagtgcc acattcactg gcattcacaa taacatgata ttcatcacgg agtgactatg
     9841 ttacagatag tcggcgcgct gattctgctg atcgcaggat ttgccattct tcgccttttg
     9901 ttcagagcat taaccagcac agcgtctgcg ctggcagggt tcatattgct gtgtctgttc
     9961 ggtccggctt tactggctgg ctatatcact gaacgcataa cccggttatt ccatattcgc
    10021 tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt
    10081 gatggtaaac atatcgcact ggaggctcat acttttgact ctgtaaaatt tattctgacc
    10141 accgctctcg ccgctggtct gctggctctt cccgtgcaga taagaaccat tcagcagaac
    10201 gggctcacac ctgaagatat cagcaaggaa attaacgggt attactgctg tttttatact
    10261 gcttttttcc ttatggcgtg ttctgcatac gcaccattga tcgcattgca gttcgatatt
    10321 tcaccctcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg
    10381 ctatgggcgg ccagccagat ccaggcgctg aaaagactga ccagtgccat cagccagaca
    10441 ctggaagaac aaccggtgct caacagtaaa tcgtggctga gcagtttgca aaacgattac
    10501 agccttcctg aaacgctgac ggagcgcatc tggctgacac tcatttcaca acggatttcc
    10561 cggggagaac tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg
    10621 tatgaaagaa acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct
    10681 gatgaactga aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat
    10741 tttctcgatc gttgcctgga cggcggtgac tggtacccct tttcagaagg ccgccgtttt
    10801 gtatcattcc accacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta
    10861 catcatgccc cggaaaatca taagccggct ccggaatggt actgctcctc tctttgtcac
    10921 gaaacagaaa cactgtgtca ggacatttat gaacgttctt acaccggttt tatttccgat
    10981 gcaacggcga atggtctgat tctcatgaaa ctgccggaaa cctggagtac aaatgagaaa
    11041 atgtttgctt ccggagggca gggacatggg tttgccgctg aacggggaaa ccatattgtc
    11101 gacagagtcc gtctgaaaaa cgcacggatc ctcggtgata ataatgccag aaatggagca
    11161 gacagactgg tcagcggaac agaaatccag acgaaatatt gttcaactgc agcccgtagc
    11221 gtcggtgcgg cattcgacgg acaaaacgga cagtatcgtt acatgggaaa tcatggtccc
    11281 atgcaactgg aagtcccccg tgatcagtat gccggcgctg tggaaaccat gaagaataag
    11341 atccgcgaag gtaaagtacc cggtgtaacc gatcccgcag aagcgtcccg gctgattcgt
    11401 cggggacatc tgacttatac ccaggcccgt aatatcaccc ggttcgggac catcgaatcg
    11461 gtcacttatg atattgccga ggggtcggtt gtcagtctgg cggccggagg gatcagtttt
    11521 gccctgacgg catcggtctt ctggctcagc accggcgatc gcgatgctgc cctgcagaca
    11581 gctgctgtcc aggcaggaaa aaccttcacc cgcacactgg ctgtctacgt cacaacccag
    11641 caacttcacc ggctcactgt tgttcagggt atgctgaagc atattgattt ttcgacggcc
    11701 agcccgactg tccggcaggc gcttcagaag gggaccggtg caggaaatat cagtgccctg
    11761 aacaaagtga tgaagggtac gctggtgaca tctctggcac tggtagctgt cacaaccagc
    11821 cctgacatga tcaaaatgtt gcggggacgg atctccggtg cgcagttcat caggaatctt
    11881 gccgtggcat cttccggtgt ggcaggtggt gctgtcgggt cagtggcggg cgggatattg
    11941 ttcagtccac tgggaccatt tggtgcactg acagggcgtg tggttggcgg tgttctgggg
    12001 ggaatgattg cctccgctgt atcaggaaaa attgccggag cgctggttga agaagatcgc
    12061 gtcaaaattc tggcaatgat tcaggagcag gtgacatggc ttgccggcag tttcctgctg
    12121 accggacatg agattgaaaa tctgaacgcg aatctggccc gtgttatcga tcagaatgct
    12181 ctggagatca ttttcgccgc cggtatacaa caacgggccg cgaccaatat gttaatcaaa
    12241 ccactggtgg tcagtatcat caggcaacgc cccgtcatgg aatatgatgc atcccatctc
    12301 ggcaagatgg ttaaccggct ggaagaagca ttccccccgg agctcccggc ataacgtgtg
    12361 ttctgctcct tttaggtttt atcacctgat ttgccagtgt catcgtcatg actcagagag
    12421 gtaaatacca tgattcacct gttcaaaacc tgcatgatta ccgccttcat tctggggtta
    12481 acgtggtctg ccccactccg ggcacaggat caacgttaca tcagtatacg caatacagat
    12541 acgatatggc tcccgggaaa tatttgtgct taccagttcc ggctggataa tggcggaaac
    12601 ggtgaaggat ttggccccct caccatcact ctgcaactca aagacaaata tggtcagacg
    12661 ctggtgacca gaaaaatgga aacggaagct tttggtgaca gtaatgccac gcgaaccaca
    12721 gacgcatttc tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat cattaaagca
    12781 actgaagaaa gtaacggcca tcgtgtcagt ctgccgttat cggttttcga tccccaggac
    12841 taccatccac tgctgattac cgtttccgga aaaaatgtta actgatccct gaagtcagtt
    12901 tcatgcttca gcctctgcca caacgtatgg cagaggctat ttatcaggag aaaccagata
    12961 tgccgggtta cacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt
    13021 tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg
    13081 tcgggttatc cgcaacgcgg aaggtcagcg acgctgacgc agccggaact ctgaagctga
    13141 tgccggaaaa cagcttaatg cgtggctcgc cagtggaggg cttctcagac aataaacttc
    13201 attgatttca tccatcaggc cgcgtcttct ccgggagacg cggctttttt catttgttgc
    13261 accagtaaat cttaaccacc gataaggagc aaagtatgca attagtcagt cgttttggtt
    13321 atgcgaacca gatacgccgt gaccgtccgc tgacacatga agagctgatg caccatgtgc
    13381 cggggatttt cggggaagag aaacacacgt cccgaagcca gaactatacg tacatcccca
    13441 ccatcaccgt actggaaagt ctgcagcggg aaggcttcca gccctttttc gcctgccaga
    13501 cccgtgtgcg cgacccgggc cgccggggat acacaaaaca catgctgcgt ctgcggcggg
    13561 ccggagagat aaacggagaa catgtccctg aaattattct gctcaactcc catgacggta
    13621 cctccagcta ccagatgctg ccgggttact tcagattcgt ctgccagaac gggtgtgtct
    13681 gtggtcagtc tctgggggaa gtgcgtgttc cgcaccgggg aaacgtggtg gagaaagtta
    13741 ttgaaggggc ttacgaagtg gtgggcgtgt ttgaccggat tgaggagaaa cgtgatgcca
    13801 tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg gcactgactt
    13861 accgttatgg tgacgaacat cagcccgtca ccaccgccga cattctgacg ccacgacgcc
    13921 gggaggatta cggtaaggac ctgtggagta cttatcagac catccaggag aatatgctga
    13981 aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt gccattcaca
    14041 acatcgacac cgatattaag ctcaaccgcg cattgtgggt gatggcagaa acgctgctgg
    14101 agagcctgcg ctga
//
LOCUS       DADLEE010000098        13964 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000098 DADLEE010000000
VERSION     DADLEE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.098"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JQH51_003209"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JQH51_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6077448.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JQH51_003210"
     CDS             2219..2767
                     /locus_tag="JQH51_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY6077449.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JQH51_003211"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JQH51_003211"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY6077450.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JQH51_003212"
     CDS             complement(3664..4854)
                     /locus_tag="JQH51_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY6077451.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQH51_003213"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQH51_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY6077452.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH51_003214"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQH51_003214"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY6077453.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQH51_003215"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQH51_003215"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY6077454.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="JQH51_003216"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="JQH51_003216"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY6077455.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQH51_003217"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQH51_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY6077456.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="JQH51_003218"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="JQH51_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY6077457.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3550 a         3563 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc ataggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DADLEE010000099        13249 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000099 DADLEE010000000
VERSION     DADLEE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.099"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="JQH51_003219"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="JQH51_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="JQH51_003220"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="JQH51_003220"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY6077458.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="JQH51_003221"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="JQH51_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4132)
                     /gene="rhaR"
                     /locus_tag="JQH51_003222"
     CDS             complement(3194..4132)
                     /gene="rhaR"
                     /locus_tag="JQH51_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY6077459.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="JQH51_003223"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="JQH51_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY6077460.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFQEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="JQH51_003224"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="JQH51_003224"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY6077461.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="JQH51_003225"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="JQH51_003225"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY6077462.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8207..9031
                     /gene="rhaD"
                     /locus_tag="JQH51_003226"
                     /pseudo
     CDS             8207..9031
                     /gene="rhaD"
                     /locus_tag="JQH51_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="JQH51_003227"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="JQH51_003227"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY6077463.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="JQH51_003228"
     CDS             9656..10102
                     /locus_tag="JQH51_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY6077464.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="JQH51_003229"
     CDS             10113..11564
                     /locus_tag="JQH51_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAY6077465.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="JQH51_003230"
     CDS             11554..12624
                     /locus_tag="JQH51_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY6077466.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13249
                     /locus_tag="JQH51_003231"
     CDS             12624..>13249
                     /locus_tag="JQH51_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY6077467.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3459 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcctgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaata
//
LOCUS       DADLEE010000100        13098 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000100 DADLEE010000000
VERSION     DADLEE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.100"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..890)
                     /locus_tag="JQH51_003232"
     CDS             complement(<1..890)
                     /locus_tag="JQH51_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6077468.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="JQH51_003233"
     CDS             1131..1931
                     /locus_tag="JQH51_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY6077469.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="JQH51_003234"
     CDS             2009..2779
                     /locus_tag="JQH51_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY6077470.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="JQH51_003235"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="JQH51_003235"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY6077471.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="JQH51_003236"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="JQH51_003236"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY6077472.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="JQH51_003237"
     CDS             5034..5756
                     /locus_tag="JQH51_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY6077473.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="JQH51_003238"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="JQH51_003238"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY6077474.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="JQH51_003239"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="JQH51_003239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY6077475.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="JQH51_003240"
     tRNA            7149..7225
                     /locus_tag="JQH51_003240"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="JQH51_003241"
     CDS             complement(7356..8402)
                     /locus_tag="JQH51_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY6077476.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="JQH51_003242"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="JQH51_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="JQH51_003243"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="JQH51_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY6077477.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="JQH51_003244"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="JQH51_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY6077478.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="JQH51_003245"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="JQH51_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="JQH51_003246"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="JQH51_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3393 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DADLEE010000101        13014 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000101 DADLEE010000000
VERSION     DADLEE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.101"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..319)
                     /locus_tag="JQH51_003247"
     CDS             complement(<1..319)
                     /locus_tag="JQH51_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY6077479.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="JQH51_003248"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="JQH51_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="with CusA, CusC and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="JQH51_003249"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="JQH51_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY6077480.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="JQH51_003250"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="JQH51_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY6077481.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="JQH51_003251"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="JQH51_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY6077482.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="JQH51_003252"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="JQH51_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY6077483.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="JQH51_003253"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="JQH51_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY6077484.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..9788
                     /locus_tag="JQH51_003254"
                     /pseudo
     CDS             7644..9788
                     /locus_tag="JQH51_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage receptor"
     gene            9905..10612
                     /locus_tag="JQH51_003255"
     CDS             9905..10612
                     /locus_tag="JQH51_003255"
                     /inference="COORDINATES: protein motif:HMM:NF024679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage N4 adsorption protein A"
                     /protein_id="HAY6077485.1"
                     /translation="MSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVYS
                     RVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRAS
                     ASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQTI
                     EPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAIN
                     QRNGERNNAFLTIGVHW"
     gene            10613..11503
                     /locus_tag="JQH51_003256"
     CDS             10613..11503
                     /locus_tag="JQH51_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY6077486.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /locus_tag="JQH51_003257"
     CDS             11686..12447
                     /locus_tag="JQH51_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6077487.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="JQH51_003258"
     tRNA            complement(12493..12569)
                     /locus_tag="JQH51_003258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13014
                     /locus_tag="JQH51_003259"
     CDS             12812..>13014
                     /locus_tag="JQH51_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY6077488.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3185 a         3376 c         3304 g         3149 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcct
//
LOCUS       DADLEE010000102        12940 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000102 DADLEE010000000
VERSION     DADLEE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.102"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(165..1616)
                     /locus_tag="JQH51_003260"
     CDS             complement(165..1616)
                     /locus_tag="JQH51_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY6077489.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1843..3261)
                     /locus_tag="JQH51_003261"
     CDS             complement(1843..3261)
                     /locus_tag="JQH51_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY6077490.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3415..3759)
                     /locus_tag="JQH51_003262"
     CDS             complement(3415..3759)
                     /locus_tag="JQH51_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY6077491.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3759..4685)
                     /gene="glsB"
                     /locus_tag="JQH51_003263"
     CDS             complement(3759..4685)
                     /gene="glsB"
                     /locus_tag="JQH51_003263"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY6077492.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4749..5684)
                     /locus_tag="JQH51_003264"
     CDS             complement(4749..5684)
                     /locus_tag="JQH51_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY6077493.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5764..6879
                     /gene="yneK"
                     /locus_tag="JQH51_003265"
     CDS             5764..6879
                     /gene="yneK"
                     /locus_tag="JQH51_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY6077494.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            7029..8219
                     /gene="ydeA"
                     /locus_tag="JQH51_003266"
     CDS             7029..8219
                     /gene="ydeA"
                     /locus_tag="JQH51_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY6077495.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8244..8909)
                     /gene="marC"
                     /locus_tag="JQH51_003267"
     CDS             complement(8244..8909)
                     /gene="marC"
                     /locus_tag="JQH51_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY6077496.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9117..9551
                     /gene="marR"
                     /locus_tag="JQH51_003268"
     CDS             9117..9551
                     /gene="marR"
                     /locus_tag="JQH51_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY6077497.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9571..9954
                     /gene="marA"
                     /locus_tag="JQH51_003269"
     CDS             9571..9954
                     /gene="marA"
                     /locus_tag="JQH51_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY6077498.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9986..10204
                     /gene="marB"
                     /locus_tag="JQH51_003270"
     CDS             9986..10204
                     /gene="marB"
                     /locus_tag="JQH51_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY6077499.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10235..11134)
                     /gene="eamA"
                     /locus_tag="JQH51_003271"
     CDS             complement(10235..11134)
                     /gene="eamA"
                     /locus_tag="JQH51_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY6077500.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11398..12516
                     /gene="ydeE"
                     /locus_tag="JQH51_003272"
     CDS             11398..12516
                     /gene="ydeE"
                     /locus_tag="JQH51_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY6077501.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12643..12738
                     /gene="mgtS"
                     /locus_tag="JQH51_003273"
     CDS             12643..12738
                     /gene="mgtS"
                     /locus_tag="JQH51_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY6077502.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3407 a         3304 c         2958 g         3271 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatatgtc aagcttacaa cattaacagg
       61 cgctgcatgt gacacctgtt aatgataaag gaatatagga tgttgtaact aacatagtga
      121 aataagaaac ccggtcgaaa ccgggttcag aagtaacggt gttattagca caacggacgt
      181 acagcttcgc gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc
      241 agaccaggca cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct
      301 accagttcct gggtaccgat aacacggtca cgatgctggc tccagagttg ctgataacgt
      361 tccagccagt gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag
      421 aacgcaatca atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc atttgccttc
      481 tgccctgcca gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg
      541 atagacagca gctgatgctt aatgtacggg ttacggaaac gcccggtgac tgcactggcg
      601 aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc gtagatggct
      661 ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt atccagccct
      721 gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt
      781 tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt gtccaggcgc
      841 agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg ttcagcggtg
      901 tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc atcgcgcgga
      961 taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg atccagccat
     1021 tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag ttcacgcaac
     1081 gcgtcgccat tgtagtcaat caactcacac ggaatgatga tccaaccttt atccagcgca
     1141 ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg atagcttacc
     1201 gctggcgcgt catcgaattt atcgcccgca tggtagctga tacccgcttc ggtggtgtta
     1261 gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc gtattcactg
     1321 tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac cgcttccccc
     1381 ttctcattca ggccacggat aatggtggtg tacagaccat cctgcgtgct cagtgacggc
     1441 gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc ggtgtgctca
     1501 ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc accaaactga
     1561 atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt tttcacaatg
     1621 ggttcccttc tgattagtca tacaacccgt ttgaattggt gcgacaggtt agcaaacttt
     1681 aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt ttatgagaaa
     1741 agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat cataaagtgg
     1801 tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa cttcctcacc
     1861 gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca gacacgtatc
     1921 ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact
     1981 accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga atggttgcag
     2041 ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat tcaccgacgg
     2101 cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat caccgacaat
     2161 cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat gtccatagtt
     2221 atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagat gttgtgtctg
     2281 tttcagcgat ggacttttca gcgcctcata aagaccggac cgtgagtaaa cctgagtcag
     2341 aaaatcaaag tcggctcgca gcgcaacttg cttcattagc aaattgatcg ctgccacgct
     2401 aaaagaaacc ataattgggc atatcgccat cgtggcaata ccgagacgtg cggagaacat
     2461 ttccggaatg gagaacggcg aaccgaccga gatatcaatc accgaatttg ccaccagtac
     2521 gatttccacc gcaccggtga caaaggtcaa cagacatgtt acctgcggcg tatagcgcac
     2581 tgcacaccag attaatgcag gcagcgggaa cgccagactc cccgctccgc caatgactac
     2641 cgaggcaata accgacacaa taaacgcgat ggcgggcatc atctgttctg ctttaaagcg
     2701 cggcagcact ccaggaatag ccaacgtcag catacaaggc acgatcaaca cgcccgttga
     2761 gaattgctca ctgaaccagt ccgcaagcaa aggccagaaa tccagactat caatactgac
     2821 cgaaccaatc gccccgacaa tagcgcataa taatgcggca atcagacagt aattaaatag
     2881 ccgtaaagca cttactggct catacttatt tttccccaga cgcttatccc gcacgaccag
     2941 taaggcgacg gtaacaataa acatcatatt ggagaaattg ataaccagtg aaaccagtcc
     3001 ccattcggtg gttatggcat cataaaccag catcgccaca taactgatcg catagtaatg
     3061 cagtcgatta agccagacat agcgggcaaa cacccccgcc attacgccat tcagcggcca
     3121 gaaaagagaa agttcgtgaa ccagccgtaa ctctgcacca ataaaataga acagcgtggt
     3181 tagcgcaaaa atagcgatgc tattacgtag cagatgacct tcctggaaca aacggaaagt
     3241 tgaaatgggg tgtacatgca ttaattatct gtccatacaa ttgcgggtgg caattatctc
     3301 aacgtgataa gtaaattctc ataaaggata gcatgttcgc taacagcggc ggcaaataaa
     3361 ggtaccctgg gaatgctatt ttatcgtttt ccgcaattct catggctgat tcatctatac
     3421 aaccagccag tgatagatta ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac
     3481 accctgcgga atgttatgcc acttagccag acaaccgcga cagcaggtcg cggtggcgtg
     3541 ctgagcgata aacaccggat gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa
     3601 tgccggagca agacgtttag cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc
     3661 tttctccagg caatactgac gctctttcat gcccagacga aagcgagagc gaaattttga
     3721 acgggataaa cgcgcgaaga gtggatcgag agactgcatt aataaaccga acgccctaac
     3781 tgtttcgtca attgttcaag gacggcgata cccgcaagcg agttacctgc atcatccagt
     3841 tccggactcc agactgcgat ggccatttca tgcggaacaa tcgccacaat accgccacca
     3901 acgccagatt tcgccggtag tcccacccgc caggcaaact cccccgcgtt ctggtacata
     3961 ccactggtcg ccatcagcgc gttaatttgc cgcgcctgca ttggcgtcac cacaggttca
     4021 tcaatatgaa tagctttccc ctgattagcc agaaagacaa acgtccgggc cagctctaca
     4081 cagctcattt tcagagcgca gtaatgaaag tagttttgca gaacggttgt cacgtcatga
     4141 tggaaattgc caaacgactt catcagccag gcgatagccg catttcgcgc ggaatgttca
     4201 aattcggaac gcgctaccac cgtatcgtat gaaatatcag acacaccgct taagccgcgc
     4261 acgacttcca gcatacgttg ccgtggtgcg cttagtcgcc cttgcaacat atcgcagacc
     4321 accagcgcac cggtattaat gaacggatta cgcggaatac cctgttccat ttccagttgc
     4381 actaaagaat tgaacggtga tccagacgga tctttgccga cgcgttgcca gatttcctct
     4441 tcggagtaat gacgcatggc gacaacgaga ctcagcactt tggaaataga ctgaatggaa
     4501 aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca gatagcaatc
     4561 cccaatcggg aaccgtctac tgtagccagc gccggaatat aatccgcgac tttaccctga
     4621 ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc catggcgact
     4681 gccactttct actcctggac cgcaggtctg aaaagacctg cgagtatatc agagctgaat
     4741 atgtcgcgtc agatccggtc tttccacact gtctggatat tacagaattc gtgtaagccg
     4801 aaatgggaaa gctcacgacc aaagccactc tttttcacgc caccaaaggc cactcgcgcg
     4861 tcgctggcac aataaccatt gataaacacc ccaccgcatt ccagacgtgc cgccatctgt
     4921 ctggcctgtg tttcgtcggt ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta
     4981 gccagttcca gcgcatgttc tgcatctttc gcaatggtga ttgcagcaac ggggccaaac
     5041 atttcttccc gaaacgcggt catttctggg gtaacattcg ccagaaccgt tggcggatag
     5101 tagttacctg ctccagccat cttttccccg cccagtaaca aacgcgcacc ctgcgccagg
     5161 gttttctcca cctgatgatg cagctcatca cgtaaatcaa aacgagccat tggtccgaga
     5221 gcgttctctt catcacgggg atcgcccatt ttcaaggctg ccgcagctga cacaaaacgt
     5281 tcggtaaatg ccgaagcaat tccctcttcg ataataaagc gttttgccgc tgcacaaacc
     5341 tgtccggtat tctgataacg tccggctacc gccgctttaa ccgccagttc cagatcggca
     5401 tcgttaagca caataaatgg atccgaaccg cccagttcca gtacgcattt tttcagtgcc
     5461 gctccagcac gaacacttcc ggtcaccgtg acagcagcaa tgcgcgagtc tttaatcatc
     5521 tgactgacac cgtcgttgtc ggcattcagc cagccatata cgccttgcgg gattcccgca
     5581 tctttaaaca cttgggcaat gagctgagca cagcccatca cattcggcgc atgtttaagt
     5641 aagtagccgt tacctgcaag aatgatggga acagcgccac gcatcacctg cctttgatag
     5701 ttcttgcagc tcaaatgccc tatatagtga tgttccgatg acttatgact atatggggca
     5761 aatatggtta cgccagtaag catctgcaat tacatatctc ttcctgatga ttttcccgct
     5821 cgtaatattg cacctcaagt aaaagaagtt ttaaaggatt ttattgatgc actcagtacc
     5881 ataatatgtg atgaagagtg gcgcacgtct ttaaacacca actcagccac aaaaaagata
     5941 tttaacaacc ttgataactt atcatatatt cagaggactt cttttcgggg taacgacaca
     6001 ctatacaatg aaaaggttca gtttaaactc acttatcctg tcaagaatgg aagacacaaa
     6061 gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga taattttcgc
     6121 catgatggag aaattaatat tttttgcgct cccaacccaa agcctgtcac tatggggcgc
     6181 gtatttcaga ccggcgtcga gcgtgtgctc tttctgttta tgaatgattt tattgaacaa
     6241 tttccaatga tcaaccttgg cgcccccatc aaaagagcgc atacaccaca tattgaaccc
     6301 ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg
     6361 aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt
     6421 cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac
     6481 agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc taacaaacat
     6541 gtacaacccg aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat
     6601 tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat
     6661 cccaatgaaa gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt
     6721 atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa
     6781 gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc
     6841 gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc
     6901 tgactcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat
     6961 tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat
     7021 aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc
     7081 tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg
     7141 acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg
     7201 catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc
     7261 gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat
     7321 cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt
     7381 tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg
     7441 ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc
     7501 gcattgtggg gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc
     7561 ttgttaccct tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt
     7621 cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa
     7681 ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa
     7741 acattgcggg attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg
     7801 gcattattgg cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa
     7861 gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg
     7921 aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg
     7981 gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat
     8041 tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt
     8101 tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt gctgcgttaa
     8161 tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag acgcaatagt
     8221 tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag gatgccgtta
     8281 ataataaact gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca
     8341 atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa
     8401 ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag
     8461 gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg
     8521 gcggtacttg gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc
     8581 tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc
     8641 cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg
     8701 ccaaatgtat ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg
     8761 tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt gcctgccagg
     8821 ccaagaaata gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc
     8881 aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat
     8941 cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc
     9001 gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt catttgactt
     9061 atacttgcct gggcaatatt accccctaca actaattact tgccaaggca actaatgtga
     9121 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
     9181 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
     9241 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
     9301 aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
     9361 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
     9421 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
     9481 aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg cttaagaaag
     9541 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
     9601 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
     9661 gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc
     9721 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
     9781 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
     9841 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
     9901 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag ctagttgata
     9961 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    10021 tattctcttt tccgcgcaga gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    10081 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    10141 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    10201 gtagtttgtt ctggccccga catctcgggg cttattaact tcccaccttt accgctttac
    10261 gccaccgcaa gccaaataca ttgatataca gcccggtcat aatgagcacc gcacctaaaa
    10321 attgcagacc cgttaagcgt tcatccaaca atagtgccgc acttgccagt ccgactacgg
    10381 gcaccagtaa cgataacggt gcaacccgcc aggtttcata gcgtcccagt aacgtccccc
    10441 agatcccata accaacaatt gtcgccacaa acgccagata catcagcgac aagatggtgg
    10501 tcatatcaat agtaaccaga ctgtgaatca tggatgcgga accatcgaga atcagcgaga
    10561 caacaaagaa gggaatgatt gggattaaag cgctccagat taccagcgac atcaccgccg
    10621 gacgcgttga gtgcgacatg atctttttat tgaagatgtt gccacacgcc caactaaatg
    10681 ctgcagccag ggtcaacata aagccgagca tcgccacatg ctgaccgttc agactatctt
    10741 cgattaacac cagtacgcca aaaatcgcta aggcgatccc cgccaattgt ttgccatgca
    10801 gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt gcctgtaaca
    10861 ccagcgaagc cagtccagca ggcataccaa agttaatggc gcagaacaga aaggcaaact
    10921 gcgcaaaact gatggttaat ccatacccca agagcaaatt cagtggtact ttcggtcgtg
    10981 caacaaaaaa gatggccgga aaagcgacca gcataaagcg caaaccggcc agcatcagcg
    11041 gtggcatgtt atgaagcccc actttgatga ccacaaaatt tagcccccat acgaccacta
    11101 ccagtagcgc caacacccca tcttttcgcg acattctacc gcctctgaat ttcatctttt
    11161 gtaagcaatc aacttagctg aatttacttt tctttaacag ttgattcgtt agtcgccggt
    11221 tacgacggca ttaatgcgca aataagtcgc tatacttcgg atttttgcca tgcgtccgat
    11281 ggttaataac aacgacgagg caagaagggc gctggtagag cgtcgtaggg ataagttcat
    11341 ttgtctgtcc ggcagtgcgt ttcgttatgt tttattttag agatgtaaag aaatagcatg
    11401 gctacgctgc catttatgac catttacttg agtcgccagt acagcctgag tgtcgatcta
    11461 atcggttatg cgatgacaat tgcgctcact attggcgtcg tttttagcct cggttttggt
    11521 atcctggcgg ataagttcga caagaaacgc tatatgttac tggcaattac cgccttcgcc
    11581 agcggtttta ttgccattcc tttagtgaat aacgtgacgc tggttgtgct cttttttgcc
    11641 ctcattaact gcgcctattc tgtttttgct accgtgctga aagcctggtt tgccgacaat
    11701 ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact acaccatgct aaacattggc
    11761 tggaccatcg gtccgccgct cggcacgctg ttggtgatgc agagcatcaa tctgcccttt
    11821 tggctggcag ctatctgttc cgcgtttccc atgcttttca ttcaaatatg ggtaaagcgc
    11881 agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt tttattacaa
    11941 gataaagcac tgttgtggtt tacctgctct ggttttctgg cttcttttgt aagcggcgca
    12001 tttgcttcat gcatttcaca atatgtgatg gtgattgctg atggggattt tgccgaaaag
    12061 gtggtcgcgg ttgttcttcc ggtgaatgct gccatggtgg ttacgttgca atattccgtg
    12121 ggccgccgac ttaacccagc taacatccgc gcgctgatga cagcaggcac cctctgtttc
    12181 gtcatcggac tggtcggttt tattttttcc ggcaacaacc tgctattgtg gggtatgtca
    12241 gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat gttgattgac
    12301 catattgcgc cgccaggaat gaaagccagc tatttttccg cccagtcttt aggctggctt
    12361 ggtgccgcga ttaacccatt agtgagtggc gtagtgctaa ccagcctgcc gccttcctcg
    12421 ctgtttgtca tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt aaaagggatt
    12481 cgtgcaagac cgtgggggca gcccgcgctt tgttgattta agtcgaacac aataaaaatt
    12541 taattcagcc ttcgtttagg ttatctctgc taatatcttt ctcattgaga tgaaaattaa
    12601 ggtaagcgag gaaacacacc acaccataaa cggaggcaaa taatgctggg taatatgaat
    12661 gtttttatgg ccgtactggg aataatttta ttttctggtt ttctggccgc gtatttcagc
    12721 cacaaatggg atgactaatg aacggagata atccctcacc taaccggccc cttgttacag
    12781 ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa ccgccagtaa accggcggtg
    12841 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
    12901 ataacggatc gttgacacag tagcatcagt tttctcaatg
//
LOCUS       DADLEE010000103        12695 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000103 DADLEE010000000
VERSION     DADLEE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.103"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQH51_003274"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQH51_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY6077503.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JQH51_003275"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JQH51_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY6077504.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JQH51_003276"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JQH51_003276"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY6077505.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JQH51_003277"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JQH51_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY6077506.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JQH51_003278"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JQH51_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY6077507.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JQH51_003279"
     CDS             4025..4615
                     /locus_tag="JQH51_003279"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY6077508.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JQH51_003280"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JQH51_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY6077509.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JQH51_003281"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JQH51_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY6077510.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JQH51_003282"
     CDS             complement(6551..6658)
                     /locus_tag="JQH51_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077511.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="JQH51_003283"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="JQH51_003283"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY6077512.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="JQH51_003284"
     CDS             7648..7875
                     /locus_tag="JQH51_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077513.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQH51_003285"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQH51_003285"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY6077514.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="JQH51_003286"
     CDS             complement(9447..10703)
                     /locus_tag="JQH51_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY6077515.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="JQH51_003287"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="JQH51_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY6077516.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="JQH51_003288"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="JQH51_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY6077517.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQH51_003289"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQH51_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY6077518.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3241 c         3199 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DADLEE010000104        12662 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000104 DADLEE010000000
VERSION     DADLEE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.104"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            128..1825
                     /locus_tag="JQH51_003290"
     CDS             128..1825
                     /locus_tag="JQH51_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY6077519.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="JQH51_003291"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="JQH51_003291"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY6077520.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="JQH51_003292"
     CDS             3115..3399
                     /locus_tag="JQH51_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY6077521.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQH51_003293"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQH51_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY6077522.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdxH"
                     /locus_tag="JQH51_003294"
     CDS             complement(4273..5157)
                     /gene="fdxH"
                     /locus_tag="JQH51_003294"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY6077523.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQH51_003295"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQH51_003295"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY6077524.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="JQH51_003296"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="JQH51_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY6077525.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="JQH51_003297"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="JQH51_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY6077526.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="JQH51_003298"
     CDS             10849..11334
                     /locus_tag="JQH51_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY6077527.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="JQH51_003299"
     CDS             11444..12433
                     /locus_tag="JQH51_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY6077528.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3341 a         3128 c         2941 g         3252 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DADLEE010000105        12648 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000105 DADLEE010000000
VERSION     DADLEE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.105"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            758..841
                     /locus_tag="JQH51_003300"
     CDS             758..841
                     /locus_tag="JQH51_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY6077529.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="JQH51_003301"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="JQH51_003301"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY6077530.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /locus_tag="JQH51_003302"
     CDS             complement(2319..3161)
                     /locus_tag="JQH51_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY6077531.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="JQH51_003303"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="JQH51_003303"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY6077532.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="JQH51_003304"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="JQH51_003304"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY6077533.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="JQH51_003305"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="JQH51_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="JQH51_003306"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="JQH51_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY6077534.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="JQH51_003307"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="JQH51_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY6077535.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="JQH51_003308"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="JQH51_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY6077536.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="JQH51_003309"
     CDS             10967..12166
                     /locus_tag="JQH51_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY6077537.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3170 c         3359 g         3007 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DADLEE010000106        12648 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000106 DADLEE010000000
VERSION     DADLEE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.106"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            28..1038
                     /locus_tag="JQH51_003310"
     CDS             28..1038
                     /locus_tag="JQH51_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY6077538.1"
                     /translation="MVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPP
                     YSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTD
                     EQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFG
                     WDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHE
                     KGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFF
                     EEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIAR
                     RT"
     gene            1039..3204
                     /locus_tag="JQH51_003311"
     CDS             1039..3204
                     /locus_tag="JQH51_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAY6077539.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="JQH51_003312"
     CDS             3218..4129
                     /locus_tag="JQH51_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAY6077540.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="JQH51_003313"
     CDS             4129..5424
                     /locus_tag="JQH51_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HAY6077541.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="JQH51_003314"
     CDS             5469..5699
                     /locus_tag="JQH51_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077542.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="JQH51_003315"
     CDS             5677..6177
                     /locus_tag="JQH51_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HAY6077543.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="JQH51_003316"
     CDS             6177..7595
                     /locus_tag="JQH51_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077544.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="JQH51_003317"
     CDS             7595..8443
                     /locus_tag="JQH51_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077545.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="JQH51_003318"
     CDS             8443..8898
                     /locus_tag="JQH51_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY6077546.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="JQH51_003319"
     CDS             8901..9593
                     /locus_tag="JQH51_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY6077547.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="JQH51_003320"
     CDS             9603..10934
                     /locus_tag="JQH51_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6077548.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..>12648
                     /locus_tag="JQH51_003321"
     CDS             10935..>12648
                     /locus_tag="JQH51_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY6077549.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEK"
BASE COUNT         3404 a         3128 c         3334 g         2782 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctcttg cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagcgtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaaga
//
LOCUS       DADLEE010000107        12389 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000107 DADLEE010000000
VERSION     DADLEE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.107"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="JQH51_003322"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="JQH51_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY6077550.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="JQH51_003323"
     CDS             complement(2005..2928)
                     /locus_tag="JQH51_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6077551.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="JQH51_003324"
     CDS             3145..4488
                     /locus_tag="JQH51_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6077552.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /locus_tag="JQH51_003325"
     CDS             4713..6368
                     /locus_tag="JQH51_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY6077553.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="JQH51_003326"
     CDS             6508..6732
                     /locus_tag="JQH51_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY6077554.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="JQH51_003327"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="JQH51_003327"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY6077555.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="JQH51_003328"
     CDS             complement(7326..8306)
                     /locus_tag="JQH51_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077556.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="JQH51_003329"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="JQH51_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY6077557.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="JQH51_003330"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="JQH51_003330"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY6077558.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="JQH51_003331"
     CDS             10315..10971
                     /locus_tag="JQH51_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY6077559.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="JQH51_003332"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="JQH51_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12389
                     /locus_tag="JQH51_003333"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="JQH51_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3230 a         2903 c         3110 g         3146 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DADLEE010000108        12240 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000108 DADLEE010000000
VERSION     DADLEE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.108"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..106)
                     /locus_tag="JQH51_003334"
     CDS             complement(<1..106)
                     /locus_tag="JQH51_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY6077560.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="JQH51_003335"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="JQH51_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY6077561.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="JQH51_003336"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="JQH51_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY6077562.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="JQH51_003337"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="JQH51_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY6077563.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="JQH51_003338"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="JQH51_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY6077564.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="JQH51_003339"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="JQH51_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="JQH51_003340"
     CDS             complement(3707..4198)
                     /locus_tag="JQH51_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY6077565.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /locus_tag="JQH51_003341"
     CDS             complement(4300..4854)
                     /locus_tag="JQH51_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY6077566.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /locus_tag="JQH51_003342"
     CDS             complement(4878..5615)
                     /locus_tag="JQH51_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY6077567.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JQH51_003343"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JQH51_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="JQH51_003344"
     tRNA            6843..6930
                     /locus_tag="JQH51_003344"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="JQH51_003345"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="JQH51_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..8647)
                     /locus_tag="JQH51_003346"
     CDS             complement(7643..8647)
                     /locus_tag="JQH51_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY6077568.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8719..9279)
                     /locus_tag="JQH51_003347"
                     /pseudo
     CDS             complement(8719..9279)
                     /locus_tag="JQH51_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9364..9561)
                     /locus_tag="JQH51_003348"
     CDS             complement(9364..9561)
                     /locus_tag="JQH51_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY6077569.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9573..10064)
                     /locus_tag="JQH51_003349"
     CDS             complement(9573..10064)
                     /locus_tag="JQH51_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077570.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10061..10435)
                     /locus_tag="JQH51_003350"
     CDS             complement(10061..10435)
                     /locus_tag="JQH51_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY6077571.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10482..10856)
                     /locus_tag="JQH51_003351"
     CDS             complement(10482..10856)
                     /locus_tag="JQH51_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY6077572.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11019..11240)
                     /locus_tag="JQH51_003352"
     CDS             complement(11019..11240)
                     /locus_tag="JQH51_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY6077573.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11303..11779)
                     /locus_tag="JQH51_003353"
     CDS             complement(11303..11779)
                     /locus_tag="JQH51_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY6077574.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11795..>12240)
                     /locus_tag="JQH51_003354"
     CDS             complement(11795..>12240)
                     /locus_tag="JQH51_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY6077575.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         3171 a         2983 c         2888 g         3198 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaactgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagcc tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact ccgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtagcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg accaacacca ggaattgtca taatgcgctg agcggtatca tcagcgttga
     8041 ttgactgact taattctgat tccagctctg ctatttgctc aacaaggtaa aagtagtggg
     8101 tatgcaacct caccaaaagg cggctcagat attcggggat ttcgtgttca gccaacacga
     8161 gagacaaccg ttttattaca gcatcaccta tcggcaggct gattccaaat tccagcaaga
     8221 aaccgtggat ctggttggtt gttttcacct tatctctgat gagcgattct ctgacccggt
     8281 gtaacgccct catggcctgc tgggtctcag ttcttggctg gacaaaacgc attgatggtc
     8341 gtgaagccgc ttcgcatatg gcttctgcat caacaaaatc attcttatta cttttgacaa
     8401 acgggcgaac gaactgtgga gaaatcagct tagtttcatg cccaaaatca gagatacagc
     8461 gagccataaa gtgtgcaccc gcacaggctt ccattacaac aactgagggc ggacatgtcg
     8521 ccagaaactg catcagttta gtacgggtaa attttttacg cagaagagcc ttaccagact
     8581 tgtcttgaca atgaatgtgg aaggagtgct ttccgagatc gataccaata agtgtgatgt
     8641 tctgcatgat ggtctctccg ttaaagggat ttaccttaac agcgtactgc tgttaaggtg
     8701 ggggtgacca tatcattatc cccaccggtc ccatctcacc agattgttga cgataatttt
     8761 actgccaaat ttacaggggc aattggtttt gtaattccag ttcagccctc tgaagacgtt
     8821 gatcacccct cgctcaaaca cctcatcctt attctggtgc agttgttcaa atgtgctcag
     8881 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
     8941 acgggcctgc gtatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
     9001 ttcgcgatca agattccggg taatggcttt cattgctgtt tcgggtttct ccatcagcca
     9061 gcagccgcag cgaaaatcct gtttcatggc ccagtccggg gcagttttac ctccgatgct
     9121 ttccgtcagc accgagattt cagccagttg tcggataagt gtgtcgattt gcaacagtgt
     9181 actctttcgt acagccagaa tgtggcgaat gctggttgag cagataacgt ccgtatgccc
     9241 gttgaccact tccggacggg agattgtgat atctgacata atcgatattc ctttttgcag
     9301 ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga
     9361 gcttcaccgg ttctggtgca gaagctgcag atgacgcagg gtacggacat cctcccgggc
     9421 ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcatcgt tgtcaaagat
     9481 aattcccgat ccgcagtcga tattgtcctg cagccacgca atcagaatat ccagcgctgt
     9541 ttccgtggtt aatgatttca tgttgttcct ggttaccagt cgaaagtggg taaacccggc
     9601 aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgccg gatgagtgtg
     9661 acgaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag aaccgggtat
     9721 gaccacgcat cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattca
     9781 ccgaagtcac tggtctggtg tgacagcgag tacaaccagg cgttgtccgt ttccgtgata
     9841 tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag
     9901 gtctggtcag attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc
     9961 agttcagcga gctcaatacc atcgacgtca acgacaatgc gccctatgtt cagtgcctgt
    10021 acgttaacgc tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact
    10081 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
    10141 ggcgagcccg gaggatatca atactgttta ttaactgaga gcggggacag gtgctgaatc
    10201 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattaact gcatcacaca
    10261 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcgacaaac ggtgtgtcat
    10321 tcagtgtgag gccataatgc tggtccagca gtcggatgag cagtgtctgc cagatggtga
    10381 cgggagacgg gcagcgagat acctcccgta catgtgtgtc ggataatgtt ttcatcttaa
    10441 gttctctgta actggtagtg ctaaaggtga gagcttcggg gttatgaggt ggttgcgggt
    10501 gctggtgtcg gataaacagc cagataaacg taaccacagg agccgagggt gtcggcttcg
    10561 caggtcagcc cttttgcata cagcgtgacg gtatgctgat atcggggatt cagttcaccg
    10621 ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatggtat
    10681 gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt cggcaaggta atacaggcgg
    10741 ttaccctctt gcaccagacg agcgccaaaa cagggcctca ctgtgcaggg aagcccccac
    10801 caggggcggt cgtgattgtc atcgggatga gttatcccgg agagtttgtc tgacacgata
    10861 aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg
    10921 accggtttcg gtgaagatgt atgcattgat gagaatggcc tcatccacag cggcgtctga
    10981 ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc agttcagggt gacactcatc
    11041 agccggacat acgggccaaa actgtcctta cggcgttctg caaacacggc aagaacgccg
    11101 ggaatatcct gcacttcacg accagtatac gcttcagcac tgccgtgcca gcgatactta
    11161 ccggtacaga acggaaaaag acgggatgca ggatgctgtt ggtgaatacg catggcttca
    11221 ccacgggtga tgattctcat gatgtgatac ctctgaagac agaagataaa agtgaaaaca
    11281 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
    11341 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
    11401 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
    11461 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
    11521 gaacttccgt acggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
    11581 ggttgttcag atacagcacc cggaactctt cccgctccag tcccgccatg ttcagaatca
    11641 gccattcccg tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt
    11701 ccagggtttg cagggcccgc agaatgagac tgcgctcgcc gggcgtcatg tttccgggca
    11761 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
    11821 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
    11881 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcag
    11941 cttccggact catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt
    12001 catcatcgtc atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat
    12061 tccagatacc accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggtt
    12121 ccagggtgac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
    12181 catcagggac gggagtggca accagttgtt gcaggccagt ctccggtgca caggccgaag
//
LOCUS       DADLEE010000109        12219 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000109 DADLEE010000000
VERSION     DADLEE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.109"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..492)
                     /locus_tag="JQH51_003355"
     CDS             complement(<1..492)
                     /locus_tag="JQH51_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077576.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="JQH51_003356"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="JQH51_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY6077577.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="JQH51_003357"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="JQH51_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY6077578.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2458..3324
                     /gene="folD"
                     /locus_tag="JQH51_003358"
     CDS             2458..3324
                     /gene="folD"
                     /locus_tag="JQH51_003358"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY6077579.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3326..3538
                     /gene="ybcJ"
                     /locus_tag="JQH51_003359"
     CDS             3326..3538
                     /gene="ybcJ"
                     /locus_tag="JQH51_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY6077580.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3646..4155
                     /locus_tag="JQH51_003360"
     CDS             3646..4155
                     /locus_tag="JQH51_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6077581.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="JQH51_003361"
     CDS             complement(4191..5576)
                     /gene="cysS"
                     /locus_tag="JQH51_003361"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY6077582.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5750..6244
                     /gene="ppiB"
                     /locus_tag="JQH51_003362"
     CDS             5750..6244
                     /gene="ppiB"
                     /locus_tag="JQH51_003362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY6077583.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6247..6969
                     /gene="lpxH"
                     /locus_tag="JQH51_003363"
     CDS             6247..6969
                     /gene="lpxH"
                     /locus_tag="JQH51_003363"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY6077584.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7087..7596
                     /gene="purE"
                     /locus_tag="JQH51_003364"
     CDS             7087..7596
                     /gene="purE"
                     /locus_tag="JQH51_003364"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY6077585.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7593..8660
                     /gene="purK"
                     /locus_tag="JQH51_003365"
     CDS             7593..8660
                     /gene="purK"
                     /locus_tag="JQH51_003365"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY6077586.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8797..9690)
                     /locus_tag="JQH51_003366"
     CDS             complement(8797..9690)
                     /locus_tag="JQH51_003366"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY6077587.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9687..10334)
                     /locus_tag="JQH51_003367"
                     /pseudo
     CDS             complement(9687..10334)
                     /locus_tag="JQH51_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10337..11569
                     /gene="allC"
                     /locus_tag="JQH51_003368"
     CDS             10337..11569
                     /gene="allC"
                     /locus_tag="JQH51_003368"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY6077588.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11580..>12219
                     /gene="allE"
                     /locus_tag="JQH51_003369"
     CDS             11580..>12219
                     /gene="allE"
                     /locus_tag="JQH51_003369"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY6077589.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3117 g         2978 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac atccgtatat aaccgattac tttatttaag
     2041 acactgatag tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca
     2101 ctctattcta aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcac
     2161 cacaaatata gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg
     2221 tatttcttaa attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg
     2281 ccatcatgta cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac
     2341 cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct
     2401 tctgacaaaa tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg
     2461 gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa
     2521 aaagttcagg ctcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg
     2581 ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc
     2641 gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag
     2701 cttatcgatg cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta
     2761 ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac
     2821 ggtttccatc cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc
     2881 accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac
     2941 gccgtggtga ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc
     3001 gcaggttgca ccaccaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa
     3061 aatgccgatc tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc
     3121 aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg
     3181 ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc
     3241 ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa
     3301 tatcatgatc cacagggtga gtaacatggc gacattttct ttaggtaaac atccgcacgt
     3361 tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat
     3421 cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa
     3481 aatcgtcgcc ggtcagacag tgagttttgc aggacacagc gtacaggttg ttgcctgatc
     3541 cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc
     3601 gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt
     3661 acgcacgccg ccgttcccct ttgcattggt ttagggttgg gttcgaaagg tctgttattt
     3721 gccggaatca tcctggcgat gctgcccgat gccgacgtat tgtcgtttaa atttggcgtt
     3781 gcttacggca atgtttttgg tcatcgcggt tttacccatt cgctggtgtt tgcgtttgtt
     3841 gtcccgctct tatgtgtgct gattggacgt cgatggttca gggctgggct gattcgctgc
     3901 tggctgtttt taaccgtctc gttgctatcg cacagcttac tggattcggt aaccactggc
     3961 ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag
     4021 gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cagcgtacgg gcatcaggtg
     4081 attatttccg aattgatgtg ggtgtggttg ccggggatgc tactgatggg aatgttgtgg
     4141 tggcgcagac gataaccgga tgcgaaaact cgcatccggc aattgcgcaa ttacttacga
     4201 cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca
     4261 cgtgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt
     4321 aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct
     4381 tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt
     4441 gcattcgccg ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac
     4501 agcacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct
     4561 tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcagtgtag
     4621 aggcgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagctggctg
     4681 cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca
     4741 tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc
     4801 attccggagt gcatccagta gttcacatac tgaccatcat gggcacaggt ggactgcgcg
     4861 atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg
     4921 ttacccagct gcttgcagtt catggccgaa cattcaatgt gccagccagg acgacccgcg
     4981 ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc
     5041 atccggttgc gtttgtcatc aaccacgtca acgcgcgcgc cagcctgcag ctggtcgaga
     5101 tcctgacgcg acagcacgcc ataagttgga tcggtcggga cgtcgaacat cacgtcgctg
     5161 ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct
     5221 gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa
     5281 tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca
     5341 ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta
     5401 tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga
     5461 ccgatatgac agagatcgta aacggtgatt ccacacacgt acatgccgac ttccccggcg
     5521 tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa
     5581 gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg
     5641 aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat
     5701 atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt
     5761 ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt
     5821 taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt
     5881 tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac
     5941 caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg gtacgctggc
     6001 aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga
     6061 taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc
     6121 tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtaa
     6181 cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga
     6241 gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc
     6301 accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt
     6361 cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag
     6421 atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc
     6481 aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg
     6541 gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg
     6601 tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa
     6661 atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgcgcg
     6721 aacagcaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgct
     6781 gtggtcagtg cgatggaaaa acatcaggtg caatggctaa tccacgggca tacccatcgc
     6841 ccggcggtgc atgaacttat cgccaaccag caacctgctt ttcgcgtggt actgggtgcc
     6901 tggcatacgg aaggttcgat ggtgaaagtc acggctgatg acgttgagct gattcatttt
     6961 ccgttttaaa aaacccacaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc
     7021 tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta
     7081 agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc
     7141 gactgggcta ccatgcagtt cgccgtcgaa atcttcgaaa tcctgaatgt cccgcaccac
     7201 gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc
     7261 gaagagaacg gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgccaggc
     7321 atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg
     7381 agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg
     7441 ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca aattctcgcg
     7501 actcacgata aagagctaca ccagcgtctg aatggctggc gcaaagccca gaccgacgaa
     7561 gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg
     7621 ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct gtctggccgg
     7681 tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga
     7741 tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga
     7801 accgcgatgt gttcccgatt attgctgacc gtctgactca gaaacagctt ttcgataagc
     7861 tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt
     7921 ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg
     7981 gtcaatggag tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt tacggtgaat
     8041 gtattgtcga gcagggcatt aacttctctg gtgaagtatc gctggttggc gcgcgcggct
     8101 ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc
     8161 gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc gaagagatgc
     8221 tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca
     8281 ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc ggtcactgga
     8341 cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc
     8401 cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt ggtagcgatg
     8461 tgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc
     8521 gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgactg
     8581 cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc gtgatttggg
     8641 cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct
     8701 tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat
     8761 caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc
     8821 ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg gtttactacg
     8881 gcttctgaca taaccactca ccgccgttac cttcggcccc atcgaaccat cggctttggc
     8941 aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca
     9001 gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat taatctgctc
     9061 ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc
     9121 cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa
     9181 ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa
     9241 atatttacca tcacgtttca tctgccagcc gtaagccgct tccagtgcct cttgttcttc
     9301 tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga
     9361 aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct
     9421 ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagtacat ccagcggata
     9481 cggttctacc tctttccacg ccagattctg taacgccagc agccccacct gcggcccgtt
     9541 gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg gtacagcact
     9601 ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt
     9661 gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc
     9721 cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc
     9781 gcaacagcgg tacgtttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg
     9841 aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga
     9901 agattgtcgg aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac
     9961 caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct
    10021 ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga
    10081 cgaaactggc gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggcccgaaa
    10141 agtccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc
    10201 ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga
    10261 atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg
    10321 aattgcgtgc ggcgaaatta cacattcccg tcaagctata gaagaaacgc tgccctggct
    10381 ttcctctttt ggcgctgacc caacgggtgg gatgacccgt ttactttatt cgccggaatg
    10441 gctggaaacc cagcagcaat ttaaaaaaag aatggcagca agtgggctgg aaacacgttt
    10501 cgatgaagtg gggaatttat acggtcgcct gagtggcacc gaatatccac aggaagtggt
    10561 tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg ggcaattcgg
    10621 cgcgctggcg gcgtggctgg caattgactg gttgaaaacg caatacggcg caccgttacg
    10681 tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt atgtcttctg
    10741 gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata tctgtgatgc
    10801 caaaggaaat agttttgccg atgcgatgaa ggcttgcgga tttactctgc cgaacgcccc
    10861 actaactccg cgtcaggcta taaaagcctt tgttgaactg catattgaac agggctgtgt
    10921 gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc agcgtcgcta
    10981 tacggtgacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg gttatcgtcg
    11041 tgatacggtt tacgctttca gtcgcatttg ccatcagtcg atcgaaaaag cgaaaaagat
    11101 gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata cggtaaatgt
    11161 ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg ccgtgctgcg
    11221 cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa tggacattgg
    11281 tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg agctggtcgc
    11341 caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc acagtggtgc
    11401 cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt ttatccccag
    11461 catcaacggg atcagccata acccggcgga acgcaccaat attactgacc ttgccgaagg
    11521 ggtcaaaacg ttggcactca tgctttatca acttgcctgg caggaataag gagtcataaa
    11581 tgggatattt aaataacgtc accggttacc gcgaagattt actggctaac cgtgcgattg
    11641 ttaaacacgg taatttcgca ctgttaaccc cggatggtct gataaaaaat attattccgg
    11701 actttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc tcttttgttg
    11761 attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt ggcgaaggca
    11821 ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa ggcaaaacat
    11881 ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg acgtttgtta
    11941 acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt ccggtagaag
    12001 gccatgcacc gtggctgttt tctggcaatg ccagcgaact ggaacgcatt cactatgaag
    12061 gtatggatga tgtgatcctg ctggattttc tgcccaaaga gttaggtttt gatatgaaca
    12121 tgcatatcct ctcttttgca ccaggtgcca gccacggtta tatcgaaaca cacgttcagg
    12181 aacatggtgc ctatattctt tccggtcagg gggtttata
//
LOCUS       DADLEE010000110        12152 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000110 DADLEE010000000
VERSION     DADLEE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.110"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            126..995
                     /locus_tag="JQH51_003370"
     CDS             126..995
                     /locus_tag="JQH51_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077590.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="JQH51_003371"
     CDS             complement(1198..1551)
                     /locus_tag="JQH51_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY6077591.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQH51_003372"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQH51_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY6077592.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="JQH51_003373"
     CDS             complement(3379..3672)
                     /locus_tag="JQH51_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY6077593.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="JQH51_003374"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="JQH51_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY6077594.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQH51_003375"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQH51_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY6077595.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="JQH51_003376"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="JQH51_003376"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY6077596.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /locus_tag="JQH51_003377"
     CDS             7587..8888
                     /locus_tag="JQH51_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY6077597.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="JQH51_003378"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="JQH51_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY6077598.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /locus_tag="JQH51_003379"
     CDS             9318..11015
                     /locus_tag="JQH51_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY6077599.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="JQH51_003380"
     CDS             11052..11627
                     /locus_tag="JQH51_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6077600.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="JQH51_003381"
     tRNA            11734..11809
                     /locus_tag="JQH51_003381"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DADLEE010000111        12113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000111 DADLEE010000000
VERSION     DADLEE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.111"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..371
                     /locus_tag="JQH51_003382"
     CDS             <1..371
                     /locus_tag="JQH51_003382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077601.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="JQH51_003383"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="JQH51_003383"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY6077602.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="JQH51_003384"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="JQH51_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY6077603.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /locus_tag="JQH51_003385"
     CDS             complement(1518..2567)
                     /locus_tag="JQH51_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY6077604.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="JQH51_003386"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="JQH51_003386"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY6077605.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="JQH51_003387"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="JQH51_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY6077606.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="JQH51_003388"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="JQH51_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY6077607.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="JQH51_003389"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="JQH51_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY6077608.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="JQH51_003390"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="JQH51_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY6077609.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="JQH51_003391"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="JQH51_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY6077610.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="JQH51_003392"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="JQH51_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY6077611.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="JQH51_003393"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="JQH51_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY6077612.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12113)
                     /locus_tag="JQH51_003394"
     CDS             complement(11913..>12113)
                     /locus_tag="JQH51_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY6077613.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3388 c         2934 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aag
//
LOCUS       DADLEE010000112        11924 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000112 DADLEE010000000
VERSION     DADLEE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.112"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..2341)
                     /locus_tag="JQH51_003395"
     CDS             complement(<1..2341)
                     /locus_tag="JQH51_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY6077614.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="JQH51_003396"
     CDS             complement(2275..2898)
                     /locus_tag="JQH51_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY6077615.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="JQH51_003397"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="JQH51_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="JQH51_003398"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="JQH51_003398"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY6077616.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /locus_tag="JQH51_003399"
     CDS             7506..8228
                     /locus_tag="JQH51_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY6077617.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="JQH51_003400"
     CDS             complement(8356..10290)
                     /gene="yfaL"
                     /locus_tag="JQH51_003400"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAY6077618.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10300..11895)
                     /locus_tag="JQH51_003401"
     CDS             complement(10300..11895)
                     /locus_tag="JQH51_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HAY6077619.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2759 a         3306 c         2943 g         2916 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtat tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt tataaaccgc caagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag ctatccagcc aggccccctg
     8881 cttctgatta ccgtgctgat accagcttga ggtcagccca acggcataac cgtggttctg
     8941 gttatcggcg cgagttccgg tcatattcga gcggctgtcg ccctgattat cactgtagcc
     9001 accaaccgcc ccaagcatcc actcgccatc cgtgccccag cgcccgctaa acaagccgcc
     9061 gctaagctgc accgtagaag tgtcttcctg ctgagccagt tgccctgccg ccgtgtaatg
     9121 ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag cgtgatcgcg
     9181 tcgctccatc ataaacgcct gatttgccgc ccgcaggtta ttgagataac cgccaacttt
     9241 ggcattcagc accggctggt aagcaggcgt aggggccggg tcgggggttg ggtcaggatc
     9301 cggcgtggga tcaggatcgg gagtcgggtc tggctcaggt ggcgatggcg gcgttacttc
     9361 ttgcgatcgc agataccagt cgttgttatc ttccactagc gtgtagtcat acgctcccat
     9421 gttgacgtag ccactgccag ccagactgaa ctgtgcattg ttttgaaact gcgtgggatc
     9481 agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga
     9541 gttaaccacc acagttgtgt tgccagcagt attaccgttc atcaccaatt gatcgcttac
     9601 cgagtcatcg ccgtttaatt cgctgtcgaa tagtagcgta ccgccgccgg tataatcacc
     9661 gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctaaccgcac cggacgtatt
     9721 ggtcagatca ccattaaccg tcacgtcagc aagataggtc aggctgtcag tttcaaggtt
     9781 ggtattaagc tcgcctgtca acgtcacgcc atcggtagca tttaccagcg tcccatcgcc
     9841 gttaaatagt gaggcattaa gggcgacgga agtatcctgc ccggtcagac gcaaaaccgt
     9901 accatcgctg atgtcgatag tgccgctgga agtagcatca attgactgaa tatcctgatc
     9961 cgcgccagta acgaacgttg ccccatcacc aacccacagt gacgaagcat aaggaaagat
    10021 atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact gcgttgtacc
    10081 gctggcggtc agctccagcg tacccgcccc cgttttagta aatgtgctac cctcatcctg
    10141 atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac
    10201 tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg tcgtagagcg
    10261 agtatcgaca ttggcagtaa tctccatcac gctttcttgt tacttatcgg taaagataac
    10321 ctggcggtta tagtgggtat ctccaagctg cgagttgtcg ctcaccatca gcgtaccgga
    10381 ggcgatttgt gttgtgccca aataactatt attgtttgcc agacttacct gcccgtcgct
    10441 cgcatcgacc accacgtaga cgccatcttt cccgtcgccg ccgatatgca aattgtcgcc
    10501 acctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc cactgacttc
    10561 aaggccatca ttgtatgatg tgccgctctg ccaagtggag aaatcagaga gatcgagcac
    10621 accgccgttt aacacgatcg actgcggatc gtcctggaga gcggtaagat ctgccgcgtc
    10681 gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta gcgccatcga
    10741 ctgcgcccct gccagcacgt agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc
    10801 gatgatgcca gcaaaactgc cctgattaac agtaacgttg ccaccagcat cgatatttaa
    10861 agtgccattc tgaaagcccg tcaatgcgtg cacaaaagtt tgttgggtcg agccaacgtt
    10921 tagctcagcc tgattctgat actgatcaat actccctatc gtcagaccgt agcagtcttg
    10981 cggatcgtcc tggcaatgcg tatcgccgac attcatcagg gagttgctgc gacccagggt
    11041 aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg cattaagtac
    11101 cagatcgccg gaacctgttt tggtgattaa cccggtacca gcaatagagt caacagctcc
    11161 gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc
    11221 acttaagccg agatacataa agccacccgc cgcagtggag ggaccatctc catagcctgc
    11281 tgcgctattg ttctcatcga ctaacacgcc gccgttctgg ctgtagctgt catcaacaga
    11341 aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc cataaccttc
    11401 ggctgtgtta tttgtaaagg cggtgttatt tattatcgta taacctgaag gatgcttgct
    11461 gtcgctatta ttatcggtaa catcgattgc cccgccatcg ccatcactgt aacttgttga
    11521 tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagacgtcat tattaatggt
    11581 ataaattgcg ccacctttgc catcattagc gatattattg atggcgttag tgacacgtaa
    11641 atcgacggca ccagtatcat tagtaccaga agaatagatt gcgccaccat agccgcctgc
    11701 gacgttaccg gaaaaaataa catcagtaag attcagcgtt gagttttctt tagcaaatat
    11761 ggccccgccg ttattatatt ctcctgtaac ggtgttattg gcaaacagag tcattccagt
    11821 ttcattttct ggtaatagtg aaaattccgc tccttgttgc aaaaatacgg ccccaccgct
    11881 ggcattattg gtcatatccg aaaaaaccag ccattgccca tcgg
//
LOCUS       DADLEE010000113        11682 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000113 DADLEE010000000
VERSION     DADLEE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.113"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..346
                     /locus_tag="JQH51_003402"
     CDS             <1..346
                     /locus_tag="JQH51_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY6077620.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="JQH51_003403"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="JQH51_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY6077621.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="JQH51_003404"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="JQH51_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY6077622.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="JQH51_003405"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="JQH51_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY6077623.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="JQH51_003406"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="JQH51_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY6077624.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="JQH51_003407"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="JQH51_003407"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY6077625.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="JQH51_003408"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="JQH51_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY6077626.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="JQH51_003409"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="JQH51_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY6077627.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="JQH51_003410"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="JQH51_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY6077628.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="JQH51_003411"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="JQH51_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY6077629.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /locus_tag="JQH51_003412"
     CDS             7292..8146
                     /locus_tag="JQH51_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAY6077630.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /locus_tag="JQH51_003413"
     CDS             8146..8832
                     /locus_tag="JQH51_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY6077631.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="JQH51_003414"
     CDS             complement(8961..9236)
                     /locus_tag="JQH51_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY6077632.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="JQH51_003415"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="JQH51_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY6077633.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="JQH51_003416"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="JQH51_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY6077634.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="JQH51_003417"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="JQH51_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY6077635.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="JQH51_003418"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="JQH51_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY6077636.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11630)
                     /locus_tag="JQH51_003419"
     CDS             complement(11073..11630)
                     /locus_tag="JQH51_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY6077637.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCCSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2767 a         2871 c         3087 g         2957 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DADLEE010000114        11655 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000114 DADLEE010000000
VERSION     DADLEE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.114"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            597..794
                     /locus_tag="JQH51_003420"
     CDS             597..794
                     /locus_tag="JQH51_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077638.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JQH51_003421"
     CDS             complement(744..1937)
                     /locus_tag="JQH51_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077639.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JQH51_003422"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JQH51_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY6077640.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JQH51_003423"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JQH51_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY6077641.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JQH51_003424"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JQH51_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY6077642.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="JQH51_003425"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="JQH51_003425"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY6077643.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="JQH51_003426"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="JQH51_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY6077644.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10955..>11655
                     /locus_tag="JQH51_003427"
     CDS             10955..>11655
                     /locus_tag="JQH51_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6077645.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DADLEE010000115        11521 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000115 DADLEE010000000
VERSION     DADLEE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.115"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(217..292)
                     /locus_tag="JQH51_003428"
     tRNA            complement(217..292)
                     /locus_tag="JQH51_003428"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="JQH51_003429"
     CDS             851..3103
                     /locus_tag="JQH51_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077646.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="JQH51_003430"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="JQH51_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY6077647.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="JQH51_003431"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="JQH51_003431"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY6077648.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="JQH51_003432"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="JQH51_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="JQH51_003433"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="JQH51_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="JQH51_003434"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="JQH51_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY6077649.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="JQH51_003435"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="JQH51_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY6077650.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="JQH51_003436"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="JQH51_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY6077651.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11279..11437)
                     /locus_tag="JQH51_003437"
     CDS             complement(11279..11437)
                     /locus_tag="JQH51_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY6077652.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2961 a         2830 c         2874 g         2856 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
    11281 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
    11341 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
    11401 gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
    11461 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
    11521 t
//
LOCUS       DADLEE010000116        11110 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000116 DADLEE010000000
VERSION     DADLEE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.116"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQH51_003438"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQH51_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY6077653.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JQH51_003439"
     tRNA            complement(621..695)
                     /locus_tag="JQH51_003439"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="JQH51_003440"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="JQH51_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="JQH51_003441"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="JQH51_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY6077654.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="JQH51_003442"
     CDS             complement(2932..3990)
                     /locus_tag="JQH51_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077655.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="JQH51_003443"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="JQH51_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY6077656.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="JQH51_003444"
     CDS             6068..6352
                     /locus_tag="JQH51_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY6077657.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="JQH51_003445"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="JQH51_003445"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY6077658.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="JQH51_003446"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="JQH51_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY6077659.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="JQH51_003447"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="JQH51_003447"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY6077660.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2777 a         2542 c         2748 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaag caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DADLEE010000117        11048 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000117 DADLEE010000000
VERSION     DADLEE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.117"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            409..1239
                     /locus_tag="JQH51_003448"
     CDS             409..1239
                     /locus_tag="JQH51_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY6077661.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQH51_003449"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQH51_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY6077662.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="JQH51_003450"
     CDS             complement(2368..3828)
                     /locus_tag="JQH51_003450"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY6077663.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQH51_003451"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQH51_003451"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY6077664.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="JQH51_003452"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="JQH51_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY6077665.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQH51_003453"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQH51_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY6077666.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQH51_003454"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQH51_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY6077667.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQH51_003455"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQH51_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY6077668.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11048)
                     /gene="fimD"
                     /locus_tag="JQH51_003456"
     CDS             complement(9005..>11048)
                     /gene="fimD"
                     /locus_tag="JQH51_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY6077669.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2686 a         2827 c         2683 g         2852 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccga
//
LOCUS       DADLEE010000118        10965 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000118 DADLEE010000000
VERSION     DADLEE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.118"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..241
                     /locus_tag="JQH51_003457"
     CDS             <1..241
                     /locus_tag="JQH51_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077670.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="JQH51_003458"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="JQH51_003458"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY6077671.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="JQH51_003459"
     CDS             2140..2856
                     /locus_tag="JQH51_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY6077672.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="JQH51_003460"
     tRNA            complement(3312..3387)
                     /locus_tag="JQH51_003460"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="JQH51_003461"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="JQH51_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="JQH51_003462"
     tRNA            5120..5195
                     /locus_tag="JQH51_003462"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="JQH51_003463"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="JQH51_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY6077673.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="JQH51_003464"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="JQH51_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY6077674.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="JQH51_003465"
     tRNA            7530..7605
                     /locus_tag="JQH51_003465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="JQH51_003466"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="JQH51_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY6077675.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="JQH51_003467"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="JQH51_003467"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY6077676.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="JQH51_003468"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="JQH51_003468"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY6077677.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10965)
                     /gene="cobU"
                     /locus_tag="JQH51_003469"
     CDS             complement(10489..>10965)
                     /gene="cobU"
                     /locus_tag="JQH51_003469"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY6077678.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3047 a         2787 c         2374 g         2757 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccatgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ttggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgtt cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt tctatccgtg cagccatctc
    10921 atcatcaagg atttgcgagg tagcgatata cagaacctgt gaaga
//
LOCUS       DADLEE010000119        10946 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000119 DADLEE010000000
VERSION     DADLEE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.119"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQH51_003470"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQH51_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY6077679.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="JQH51_003471"
     CDS             complement(1433..1759)
                     /locus_tag="JQH51_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY6077680.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="JQH51_003472"
     CDS             1894..2235
                     /locus_tag="JQH51_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY6077681.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="JQH51_003473"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="JQH51_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="JQH51_003474"
     CDS             complement(2833..3543)
                     /locus_tag="JQH51_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY6077682.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="JQH51_003475"
     CDS             3651..3956
                     /locus_tag="JQH51_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY6077683.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="JQH51_003476"
     CDS             4155..4475
                     /locus_tag="JQH51_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HAY6077684.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="JQH51_003477"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="JQH51_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="JQH51_003478"
     CDS             complement(6299..7288)
                     /locus_tag="JQH51_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077685.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="JQH51_003479"
     CDS             complement(7373..7864)
                     /locus_tag="JQH51_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1682 domain-containing protein"
                     /protein_id="HAY6077686.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="JQH51_003480"
     CDS             complement(8185..8685)
                     /locus_tag="JQH51_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077687.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="JQH51_003481"
     CDS             complement(8675..9190)
                     /locus_tag="JQH51_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077688.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="JQH51_003482"
     CDS             complement(9621..9989)
                     /locus_tag="JQH51_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077689.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..10889)
                     /locus_tag="JQH51_003483"
     CDS             complement(10401..10889)
                     /locus_tag="JQH51_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6077690.1"
                     /translation="MVSVVTGARSAEVCHLTKRDIVTLDNGLVCIDINEDGDGKSVKN
                     KHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTPSAYSSWFNSRVLTEALGDS
                     QDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDLYGKGHAVGRLADVLKTA
                     LL"
BASE COUNT         2811 a         2783 c         2704 g         2648 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga ggacatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca
    10861 gaccgagcgc cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg
    10921 tgagccgtgt gtcgtgtgaa ctgata
//
LOCUS       DADLEE010000120        10927 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000120 DADLEE010000000
VERSION     DADLEE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.120"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            155..505
                     /locus_tag="JQH51_003484"
     CDS             155..505
                     /locus_tag="JQH51_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY6077691.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            558..953
                     /locus_tag="JQH51_003485"
     CDS             558..953
                     /locus_tag="JQH51_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077692.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="JQH51_003486"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="JQH51_003486"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY6077693.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="JQH51_003487"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="JQH51_003487"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY6077694.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /locus_tag="JQH51_003488"
     CDS             complement(2870..5299)
                     /locus_tag="JQH51_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY6077695.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="JQH51_003489"
     CDS             complement(5324..6424)
                     /locus_tag="JQH51_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY6077696.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="JQH51_003490"
     CDS             complement(6812..7558)
                     /gene="cutC"
                     /locus_tag="JQH51_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY6077697.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7572..8138)
                     /locus_tag="JQH51_003491"
     CDS             complement(7572..8138)
                     /locus_tag="JQH51_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY6077698.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8354..10087
                     /gene="argS"
                     /locus_tag="JQH51_003492"
     CDS             8354..10087
                     /gene="argS"
                     /locus_tag="JQH51_003492"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY6077699.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10264..10752
                     /locus_tag="JQH51_003493"
     CDS             10264..10752
                     /locus_tag="JQH51_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY6077700.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2670 a         2788 c         2605 g         2864 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattt gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
     6481 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
     6541 atttattaca atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat
     6601 atttatcagt tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag
     6661 cacaacgcta tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata
     6721 ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat
     6781 catattgggc gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc
     6841 ctttcatttc agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt
     6901 catctgatga catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc
     6961 ccgcggagct atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac
     7021 ggactcctgc tccagccata atgattggag catcacgatg ggcaataagt tccataattt
     7081 ttgataaacc ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca
     7141 attccgcaag attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa
     7201 aagtcactgc cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat
     7261 tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc
     7321 gcacatcctc aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg
     7381 ggcgaattat cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca
     7441 acgacggcgt taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt
     7501 tttgctgcgc cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt
     7561 ttgctcctta attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga
     7621 aacttaatag ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc
     7681 tcaccttttg gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa
     7741 agcaatgcca gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata
     7801 tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa
     7861 aactgccagt gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca
     7921 ttgatcattt tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca
     7981 gcggtgacgt tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc
     8041 aaccccagac ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca
     8101 atatcctgca gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta
     8161 aaagcgttaa tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg
     8221 atgcaatagt gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc
     8281 agtatactcc cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat
     8341 gtaaggtatt ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat
     8401 tgcggcaggc gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt
     8461 cggcgactat caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg
     8521 acaattagca gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga
     8581 gatcgccggt ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt
     8641 tcagcaggcg ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt
     8701 ggttgactac tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac
     8761 cattattggt gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc
     8821 aaaccacgtc ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca
     8881 gcagcaggaa aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc
     8941 gaaaaagcat tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa
     9001 actgcaaagc ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat
     9061 gacgcagaac cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat
     9121 gggcgaaagc ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg
     9181 tctggcagta gaaagcgaag gggcgaccgt cgtattcctt gatgtgttta aaaacaagga
     9241 aggcgaaccg atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac
     9301 tgatatcgcc tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta
     9361 catcgactcc cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg
     9421 ctatgtaccg gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga
     9481 cggcaaaccg ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga
     9541 agccctggag cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga
     9601 gctggaaaaa ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa
     9661 aaaccgcacc acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac
     9721 cgcgccatac atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat
     9781 taacgaagag caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact
     9841 ggcagctcgc ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc
     9901 gcatgtaatg tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca
     9961 ctgcccgatc ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca
    10021 actgacagcg aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg
    10081 tatgtaatcg atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt
    10141 attgccatct aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag
    10201 aggccagaag cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc
    10261 gaagtgttta aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa
    10321 gatacggtta tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat
    10381 tcagatgatg atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc
    10441 gtcgatgcgc aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca
    10501 cgcacaggtg caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga
    10561 gcctggctgg attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag
    10621 gatttaagtg acccctattc tgaatcagaa tggctatcat gtatgatcat acagaccaat
    10681 acgcgaactc gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg
    10741 acaagaggat aattcacatc tttttggcat gttttgttgc aagctattcc tgataaatga
    10801 ttgcaacaaa acatccagcc ttttgactga gttattaaac atactcgcga gcgcgtaatt
    10861 ttttgtcttt cagcgataat tcacaatcac ttcattccgt tgtaccttta gcggtggaat
    10921 taaccgc
//
LOCUS       DADLEE010000121        10556 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000121 DADLEE010000000
VERSION     DADLEE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.121"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            38..778
                     /locus_tag="JQH51_003494"
     CDS             38..778
                     /locus_tag="JQH51_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077701.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="JQH51_003495"
     CDS             837..1223
                     /locus_tag="JQH51_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY6077702.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="JQH51_003496"
     CDS             1232..1561
                     /locus_tag="JQH51_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY6077703.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="JQH51_003497"
     CDS             1533..4574
                     /locus_tag="JQH51_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY6077704.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="JQH51_003498"
     CDS             4574..4903
                     /locus_tag="JQH51_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077705.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="JQH51_003499"
     CDS             4903..5601
                     /locus_tag="JQH51_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY6077706.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="JQH51_003500"
     CDS             5606..6349
                     /locus_tag="JQH51_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077707.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..6888
                     /locus_tag="JQH51_003501"
     CDS             6286..6888
                     /locus_tag="JQH51_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY6077708.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="JQH51_003502"
     CDS             6949..10428
                     /locus_tag="JQH51_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY6077709.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..>10556
                     /locus_tag="JQH51_003503"
     CDS             10496..>10556
                     /locus_tag="JQH51_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HAY6077710.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2487 a         2498 c         3388 g         2183 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgc
//
LOCUS       DADLEE010000122        10464 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000122 DADLEE010000000
VERSION     DADLEE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.122"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="JQH51_003504"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="JQH51_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY6077711.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="JQH51_003505"
     CDS             214..402
                     /locus_tag="JQH51_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY6077712.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="JQH51_003506"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="JQH51_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY6077713.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="JQH51_003507"
     CDS             597..770
                     /locus_tag="JQH51_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY6077714.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="JQH51_003508"
     CDS             complement(819..1892)
                     /locus_tag="JQH51_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY6077715.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4660)
                     /gene="torS"
                     /locus_tag="JQH51_003509"
                     /pseudo
     CDS             complement(1994..4660)
                     /gene="torS"
                     /locus_tag="JQH51_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="JQH51_003510"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="JQH51_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY6077716.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="JQH51_003511"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="JQH51_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY6077717.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="JQH51_003512"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="JQH51_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY6077718.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="JQH51_003513"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="JQH51_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="JQH51_003514"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="JQH51_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY6077719.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tggctgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DADLEE010000123        10399 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000123 DADLEE010000000
VERSION     DADLEE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.123"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            415..546
                     /locus_tag="JQH51_003515"
     CDS             415..546
                     /locus_tag="JQH51_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY6077720.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            806..1801
                     /gene="pgl"
                     /locus_tag="JQH51_003516"
     CDS             806..1801
                     /gene="pgl"
                     /locus_tag="JQH51_003516"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY6077721.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1842..2795)
                     /locus_tag="JQH51_003517"
                     /pseudo
     CDS             complement(1842..2795)
                     /locus_tag="JQH51_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2979..4031
                     /locus_tag="JQH51_003518"
     CDS             2979..4031
                     /locus_tag="JQH51_003518"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY6077722.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4107..5540
                     /locus_tag="JQH51_003519"
     CDS             4107..5540
                     /locus_tag="JQH51_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY6077723.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5723..7903
                     /locus_tag="JQH51_003520"
     CDS             5723..7903
                     /locus_tag="JQH51_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY6077724.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8044..9327)
                     /locus_tag="JQH51_003521"
     CDS             complement(8044..9327)
                     /locus_tag="JQH51_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY6077725.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9462..10343)
                     /locus_tag="JQH51_003522"
     CDS             complement(9462..10343)
                     /locus_tag="JQH51_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6077726.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2545 a         2405 c         2598 g         2851 t
ORIGIN      
        1 gccataaggg aatgagtgtt atatcatgct ttctacccta gtaagtatat ttgctttttt
       61 cgtatatttg gttcatcttt ttttgatgtt ttacatattc acaatgcaga ggatttatac
      121 cagaggacat tagtcttgag taagatacta caacatctcc aactatttta gccagcctga
      181 ctacttgact tggtaatatt tgttgctaat aatcatgtat aaaataagct tatatacata
      241 gtaacttcag caaaggattt agtaatgatc aaaatcatat ctcaatgtac tgtttttttc
      301 tttaaaaaaa cagtacattc tattttataa tgactgtgta ttcccatgga aggagatcac
      361 ccaattctcc cccccgactt ttgacgcagt gtcttgttaa cagggggatt gaccatgctt
      421 attggctatg tacgcgtatc aacaaatgac cagaataccg atctacaacg taatgcgttt
      481 atttgtgccg gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
      541 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      601 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      661 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      721 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      781 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      841 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      901 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      961 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
     1021 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
     1081 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
     1141 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
     1201 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
     1261 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
     1321 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
     1381 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
     1441 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1501 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1561 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1621 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1681 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1741 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1801 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1861 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1921 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1981 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     2041 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     2101 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     2161 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     2221 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     2281 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     2341 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     2401 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     2461 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2521 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2581 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2641 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2701 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2761 acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac
     2821 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2881 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2941 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     3001 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     3061 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     3121 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     3181 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     3241 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     3301 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     3361 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     3421 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3481 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3541 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3601 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3661 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3721 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3781 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3841 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3901 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3961 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     4021 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     4081 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     4141 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     4201 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     4261 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     4321 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     4381 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     4441 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4501 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4561 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4621 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4681 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4741 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4801 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4861 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4921 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4981 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     5041 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     5101 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     5161 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     5221 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     5281 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     5341 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     5401 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     5461 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5521 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5581 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5641 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5701 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5761 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5821 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5881 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5941 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     6001 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     6061 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     6121 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     6181 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     6241 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     6301 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     6361 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     6421 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6481 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6541 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6601 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6661 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6721 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6781 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6841 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6901 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6961 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     7021 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     7081 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     7141 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     7201 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     7261 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     7321 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     7381 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     7441 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7501 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7561 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7621 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7681 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7741 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7801 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7861 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7921 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7981 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     8041 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     8101 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     8161 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     8221 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     8281 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     8341 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     8401 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     8461 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8521 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8581 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8641 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8701 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8761 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8821 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8881 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8941 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     9001 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     9061 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     9121 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     9181 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     9241 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     9301 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     9361 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     9421 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9481 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9541 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9601 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9661 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9721 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9781 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9841 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9901 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9961 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
    10021 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
    10081 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
    10141 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
    10201 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
    10261 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
    10321 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
    10381 gcctgtatag cttcagtga
//
LOCUS       DADLEE010000124        10370 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000124 DADLEE010000000
VERSION     DADLEE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.124"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..336)
                     /locus_tag="JQH51_003523"
     CDS             complement(<1..336)
                     /locus_tag="JQH51_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY6077727.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="JQH51_003524"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="JQH51_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="JQH51_003525"
     CDS             complement(1130..1336)
                     /locus_tag="JQH51_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY6077728.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="JQH51_003526"
     CDS             complement(1443..2006)
                     /locus_tag="JQH51_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY6077729.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="JQH51_003527"
     CDS             complement(3381..5216)
                     /locus_tag="JQH51_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077730.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="JQH51_003528"
     CDS             5317..5604
                     /locus_tag="JQH51_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY6077731.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="JQH51_003529"
     CDS             5576..7105
                     /locus_tag="JQH51_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY6077732.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="JQH51_003530"
     CDS             complement(7275..8492)
                     /locus_tag="JQH51_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6077733.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="JQH51_003531"
     CDS             complement(8859..9845)
                     /locus_tag="JQH51_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077734.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10166)
                     /locus_tag="JQH51_003532"
                     /pseudo
     CDS             complement(9842..10166)
                     /locus_tag="JQH51_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3231 a         2371 c         1949 g         2819 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc
//
LOCUS       DADLEE010000125        10043 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000125 DADLEE010000000
VERSION     DADLEE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.125"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..807
                     /locus_tag="JQH51_003533"
     CDS             <1..807
                     /locus_tag="JQH51_003533"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6077735.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="JQH51_003534"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="JQH51_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY6077736.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="JQH51_003535"
     CDS             2629..3948
                     /locus_tag="JQH51_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY6077737.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4325..5470
                     /locus_tag="JQH51_003536"
     CDS             4325..5470
                     /locus_tag="JQH51_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6077738.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            5395..6012
                     /locus_tag="JQH51_003537"
                     /pseudo
     CDS             5395..6012
                     /locus_tag="JQH51_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            6270..6602
                     /gene="ddpX"
                     /locus_tag="JQH51_003538"
                     /pseudo
     CDS             6270..6602
                     /gene="ddpX"
                     /locus_tag="JQH51_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6616..7512
                     /locus_tag="JQH51_003539"
     CDS             6616..7512
                     /locus_tag="JQH51_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY6077739.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            7509..8483
                     /locus_tag="JQH51_003540"
     CDS             7509..8483
                     /locus_tag="JQH51_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY6077740.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            8476..9402
                     /locus_tag="JQH51_003541"
                     /pseudo
     CDS             8476..9402
                     /locus_tag="JQH51_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9458..9889)
                     /gene="osmC"
                     /locus_tag="JQH51_003542"
     CDS             complement(9458..9889)
                     /gene="osmC"
                     /locus_tag="JQH51_003542"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY6077741.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2425 a         2485 c         2540 g         2593 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtacc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggattctttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct gggtccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc gcgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttacctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcgaagac cttcttccct gacttctcta
     1561 aagtgggcac actggttgta tttgttgcct tcattttgag ttatatgggc gtagaagctt
     1621 ccgcaactca cgtcaatgaa atgagcaacc ccgggcgcga ctatccactg gctatgttac
     1681 tgctgatggt ggcggcaatc tgcttaagct ctgttggcgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcgcagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggca attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 attctaccga tatgtatgtt gaattactgg ttgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgcttgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcacatat ggatatctgc
     2641 tcccgaaaca agaaattaac gattagaaga ccagcgatac tggttgcatt ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtt
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaatcccac cagccgagcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tccaacgtct cggcataaac
     2941 acggtctttt tccaggtcaa gccggacggt accgccctgt ggccatcgaa aattttgccg
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattc
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc tgaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga atcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgt aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaagg tatcacacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgcagacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaacca
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac taccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtctt
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 gatctcgcac gttgcgtttc gttttgattc cgtctgattt cccagcctta taagggtgtg
     4321 aattatggag atgtatttta aaagaatgaa agatgagtgg accggacttg tcgaacaggc
     4381 agatcctctc attcgtgcta aagccgcaga aattgccctt gcgcatgctc attatctgag
     4441 tatcgagttt tatcgaattg tccgcatcga cccgcatgcc gaagaattct tgagtaatga
     4501 acaagttgag cggcagttga aaagtgcgat ggaacgctgg attattaacg tgctttctgc
     4561 ccaggttgac gatgtcgaaa ggctaataca aatccagcat accgtcgcgg aagtgcatgc
     4621 ccgcatagga attccggtag aaattgtcga gatggggttt cgggtgctga aaaaaatcct
     4681 ctatccggta atcttctctt cggattattc cgccgcagaa aaacttcagg tctaccattt
     4741 ctcgattaac agtattgata tcgcgatgga agtgatgacc cgcgcgttta cctttagtga
     4801 cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc tcgttactgg aaaacgccga
     4861 agaagaaaaa gaacggcaaa tagcctcaat actttcatgg gaaatagata ttatctataa
     4921 agtcctgctg gattctgatt taggcagcag tttgccttta agccaggctg attttggcct
     4981 gtggtttaac cataaaggtc gacattattt tagcggcatt gccgaagtgg gccatatctc
     5041 ccgtctgatt caggatttcg acggtatttt caatcaaacc atgcgtaaca ccaggatttt
     5101 gaataacaga agtttgcggg tgaaatttct attacagata agaaataccg tatcgcagat
     5161 tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc cacgaagtcg gtatggatgt
     5221 actgacgaaa ttacttaacc gccgtttcct gccgactatc ttcaaacgcg aaattgccca
     5281 tgccaaccgg accggtacac cgctgtcagt gctgattatt gacgttgata aattcaaaga
     5341 gatcaacgat acgtggggcc ataacactgg cgatgaaatt ctgcgtaaag tctcattcct
     5401 ctcgcagaag agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc
     5461 cgtcagttag cagaatggcg tagccagaat attcatatcc cggcgttatc cgtgaacttg
     5521 tcggcgctgc actttcgcag taatcagctg cctaatcagg tgtctgatgc aatgcaagcc
     5581 tggggtattg acggccacca gctgacggtg gaaatcacgg aaagcatgat gatggaacac
     5641 gataccgaaa tctttaagcg cattcagatc ctgcgtgata tgggcgtggg tttatcggta
     5701 gatgattttg gcacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa
     5761 atcaaaattg acaaaagctt tgtcgatcgt tgtctgacgg aaaaacgcat cctggcctta
     5821 cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggggtc
     5881 gaaaccaaag agcaatttga gatgctacgt aagatccact gtcgcgttat tcagggatat
     5941 ttcttttccc gcccccttcc cgccgaagaa attccaggct ggatgagcag cgtgttaccg
     6001 ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt tttcctttaa ggtgacactg
     6061 tcacttaaat actgtgattt cagctacgat tccgggagat tccttcctta actttccctc
     6121 ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa aagttaaaaa atataacttt
     6181 aagccagcat tctggcgctt atcccggcat ggcatgagat ctgcataagc ggaaagcgca
     6241 gcaatttttg tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag
     6301 ccgtgatctt ccctgatctg gagatcgaat tgaaatatgc ctgcgttgat aacatcacag
     6361 gtaaagctat ttatcagcaa gcgcgttgtc tgctacataa agatgcgatt accgcgctgg
     6421 cgaaaagtat cagtatcgcc cagctgtcag gcttacaact ggtgatttac gatgcgtatc
     6481 gcccacaaca agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg
     6541 atgtgacggt cggttctaat cacagccgtg gcacggcgct cgacctgacg cttcgtgatg
     6601 acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa
     6661 acgcgattta acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc
     6721 ggcgcagtca tcattgtatt aatgcgactg atgatgattt tttcaccgtg gctggcgacg
     6781 catgatccca acgccattga tttaaccgcc cgccttttgc cgccttctgc ggcgcactgg
     6841 tttggcaccg atgaagtggg acgcgatctg tttagccgcg tactggtcgg cagtcagcaa
     6901 tcaattctcg ccggattagt ggtggtcgcc attgcgggta tgattggttc gctactcgga
     6961 tgtctatccg gtgtgcttgg cggacgcgca gacgccatca tcatgcgcat catggacatt
     7021 atgctgtcga ttccttcgtt ggtactgaca atggcactgg ctgccgctct cgggccgagt
     7081 ttatttaacg ccatgctggc gattgctatt gtgcgaattc ccttttatgt gcgcttggcg
     7141 cgggggcaag cattagttgt acgccagtat acctatgttc aggcggcgaa aacctttggc
     7201 gcgtctcgtt ggcatctgat caactggcat attttacgta actccctacc gccgctgatc
     7261 gtgcaggcat cgctggatat cggtagcgcg attttaatgg ccgccacgtt gggatttatt
     7321 ggcctgggtg ctcaacaacc gagtgctgaa tggggcgcga tggtagcgaa tggtcgcaac
     7381 tatgtactcg atcagtggtg gtattgcgca tttccggggg cagcgatttt gcttacctcc
     7441 gtcgggttta atctctttgg tgatggtatt cgcgatctgc ttgacccgaa agcaggagga
     7501 aagcagtcat gacccaaccc gttctggaca ttcaacagct gcatttgagt ttccccggtt
     7561 ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca gattaaccgc ggcgaaattg
     7621 tcggtctggt gggagaatcc ggctcaggta aatcagtcac cgcaatgctg attatgcgtc
     7681 tgctaccgac gggcagttat tgcgtacatc gggggcagat ttcactgctg ggagaagatg
     7741 ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg cgcacgagtg gcgatgatct
     7801 ttcaggaacc gatgaccgcc cttaatccga cacgtcgaat aggtcttcag atgatggacg
     7861 taatccgcca tcatcaacca ataagtcgtc gggaagccag agctaaagcg attgcactgc
     7921 tggaagagat gcaaatcccg gatgccgtgg aagttatgtc gcgctatccg tttgagcttt
     7981 caggtggtat gcgccagcgg gtaatgattg cgccggcatt ctcctgcgag ccgcaattga
     8041 ttattgccgg cgaaccgacg acggcgctgg acgtcacagt acagttgcag gtactgcgtt
     8101 tgcttaaaca taaagcacgc gccagtggaa ctgcggtact gttcatcagc catgatatgg
     8161 ccgtggtgtc gcaactgtgc gatagcgttt acgtgatgta tgccggaagc gtaattgaaa
     8221 gcggcgggac ggcagacgtt atccatcatc cccggcatcc gtataccatt ggtttgctgc
     8281 aatgcgcacc agaacatgga gtaccacgcc agccattacc cgccattcca gggacggtac
     8341 caaacctcac ccatttgcct gacggctgcg cttttcgcga tcgttgctat gcggcaggtg
     8401 cacagtgtgc gctgacagcg tgtggtgaca acaaccagca ctgcacctgt tggtatccgc
     8461 agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttt
     8521 ccggcccgta aaaactggct tggcaaatct acggaacatg ttcatgccat taatggtatt
     8581 gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa
     8641 agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca gtacatccgt
     8701 tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc gcttaatccg
     8761 cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg tagtagtgaa
     8821 caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg tccggagtat
     8881 ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccaga
     8941 gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc gctggatatc
     9001 tccgtgcagg cgcagatcct caatttgctg gtaacgctac aggaaaatca cgggctgact
     9061 tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg ggtggcggtg
     9121 atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac tgcttcggca
     9181 catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc gctggaggaa
     9241 gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca aggctgtttt
     9301 ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc attagcaata
     9361 agggaggacg gacgtgacct ccggtgctgg cgggcgctgt agagatgcta acaacgcatc
     9421 aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac tggtaatcca
     9481 gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct ttctggatga
     9541 tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc agtgcgattt
     9601 tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg gtatcaattg
     9661 atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg
     9721 catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca aaacgcgtgt
     9781 taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt cccttcccgc
     9841 gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc
     9901 tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata
     9961 ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc ccacaataga
    10021 taaccgggaa tatatatctc aag
//
LOCUS       DADLEE010000126         9935 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000126 DADLEE010000000
VERSION     DADLEE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.126"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1023)
                     /locus_tag="JQH51_003543"
     CDS             complement(<1..1023)
                     /locus_tag="JQH51_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY6077742.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="JQH51_003544"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="JQH51_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY6077743.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="JQH51_003545"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="JQH51_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY6077744.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="JQH51_003546"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="JQH51_003546"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY6077745.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="JQH51_003547"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="JQH51_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY6077746.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="JQH51_003548"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="JQH51_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY6077747.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="JQH51_003549"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="JQH51_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY6077748.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="JQH51_003550"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="JQH51_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY6077749.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9935)
                     /locus_tag="JQH51_003551"
     CDS             complement(8990..>9935)
                     /locus_tag="JQH51_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6077750.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2562 a         2587 c         2294 g         2492 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aaccttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DADLEE010000127         9907 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000127 DADLEE010000000
VERSION     DADLEE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.127"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(160..900)
                     /gene="pflA"
                     /locus_tag="JQH51_003552"
     CDS             complement(160..900)
                     /gene="pflA"
                     /locus_tag="JQH51_003552"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY6077751.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="JQH51_003553"
     CDS             complement(1092..3374)
                     /gene="pflB"
                     /locus_tag="JQH51_003553"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY6077752.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3429..4286)
                     /gene="focA"
                     /locus_tag="JQH51_003554"
     CDS             complement(3429..4286)
                     /gene="focA"
                     /locus_tag="JQH51_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY6077753.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4693..6453)
                     /locus_tag="JQH51_003555"
     CDS             complement(4693..6453)
                     /locus_tag="JQH51_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY6077754.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6696..7209
                     /locus_tag="JQH51_003556"
                     /pseudo
     CDS             6696..7209
                     /locus_tag="JQH51_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7408..8496
                     /gene="serC"
                     /locus_tag="JQH51_003557"
     CDS             7408..8496
                     /gene="serC"
                     /locus_tag="JQH51_003557"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY6077755.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8567..9850
                     /gene="aroA"
                     /locus_tag="JQH51_003558"
     CDS             8567..9850
                     /gene="aroA"
                     /locus_tag="JQH51_003558"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY6077756.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2368 c         2483 g         2535 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
      541 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg
      601 aatgccttct tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc
      661 ttcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
      721 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc
      781 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
      841 acctgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
      901 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
      961 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
     1021 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
     1081 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
     1141 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
     1201 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
     1261 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
     1321 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
     1381 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
     1441 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
     1501 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
     1561 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
     1621 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc
     1681 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta
     1741 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
     1801 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
     1861 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
     1921 tccatgaagt gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc
     1981 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
     2041 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
     2101 atcatcgggc ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc
     2161 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
     2221 ttgaagttca gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc
     2281 cccatagtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca
     2341 tcgaggccca taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca
     2401 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
     2461 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
     2521 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
     2581 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
     2641 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
     2701 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
     2761 tgtagagagg tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca
     2821 acgcgacggt agtcaccgat gatacggcca cggccataag catctggcag accggtcaga
     2881 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
     2941 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
     3001 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
     3061 ggagcttcag tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg
     3121 tgagaggtga tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt
     3181 tccagtttaa caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
     3241 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
     3301 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
     3361 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
     3421 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
     3481 aacaaaccac cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag
     3541 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
     3601 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
     3661 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
     3721 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gtttgccagg
     3781 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
     3841 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
     3901 acaaacagca gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac
     3961 tgacaccagg tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag
     4021 agatcggctc cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc
     4081 agttttgcca tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg
     4141 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
     4201 ttcgttgctt tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa
     4261 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
     4321 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
     4381 ccgtaactcg catggttttt atgcaaatac ggagtaacta tttgattatc caaaataaaa
     4441 ataaatttta aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt
     4501 aattaaaagg gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa
     4561 attaccgata tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg
     4621 gggcattaaa aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta
     4681 ggctatttga ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc
     4741 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc
     4801 gtaaaacgcc gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta
     4861 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa
     4921 taacaacgtt tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt
     4981 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
     5041 cagcatggct tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc
     5101 cagacccaac agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
     5161 gatgaggttc aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc
     5221 acgtaaatgg ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc
     5281 ggacatgcca ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat
     5341 ataaacttct ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt
     5401 ggtgccggag aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca
     5461 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
     5521 ttcatcatcg aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag
     5581 cagctcggtc acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc
     5641 aaagcaggtg ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc
     5701 aagggaacca tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc
     5761 aaccaccgct gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     5821 agcaataatg cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc
     5881 ttcgttgcgg gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt
     5941 catcggaata taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc
     6001 acgatcttcg ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg
     6061 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct
     6121 cagtgggaac catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc
     6181 cagccagaaa tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc
     6241 agaagccagc gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     6301 tttgtcgcga atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc
     6361 aatctgaaag ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc
     6421 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     6481 gcgcaataat acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt
     6541 tgactatgct tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc
     6601 caccgtatgg catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     6661 ctttatacct ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     6721 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     6781 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     6841 tataccgcct ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     6901 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     6961 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     7021 gtgaaaccgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     7081 gagtcggcgg actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa
     7141 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     7201 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     7261 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     7321 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     7381 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     7441 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     7501 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     7561 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     7621 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     7681 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     7741 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     7801 aagccaatgc gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat
     7861 gaaaccatcg acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc
     7921 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     7981 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     8041 gaagatttgc tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc
     8101 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     8161 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     8221 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     8281 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     8341 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc
     8401 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     8461 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     8521 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     8581 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     8641 taaccgcgct ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct
     8701 ggatagcgac gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac
     8761 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     8821 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     8881 tctttgtctg gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     8941 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     9001 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     9061 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     9121 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     9181 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     9241 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     9301 ggcttcttac ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     9361 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     9421 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     9481 tatggatatg aaccatattc ctgatgcggc gatgaccatt gccacggcgg cgttatttgc
     9541 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     9601 gtttgcgatg gcaacagaac tgcgtaaagt cggcgcggaa gtggaagagg ggcacgatta
     9661 cattcgtatc actcctccgg aaaaactgaa ctttgccgag atcgcgacat acaatgatca
     9721 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     9781 tcccaaatgc acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     9841 ggcagcctga atgaacaacg ggcaataaat agccaaatct ttctttatca aaacgtcggc
     9901 acattgt
//
LOCUS       DADLEE010000128         9774 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000128 DADLEE010000000
VERSION     DADLEE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.128"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="JQH51_003559"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="JQH51_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY6077757.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="JQH51_003560"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="JQH51_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY6077758.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="JQH51_003561"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="JQH51_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY6077759.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="JQH51_003562"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="JQH51_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY6077760.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="JQH51_003563"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="JQH51_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY6077761.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="JQH51_003564"
     CDS             complement(6031..6360)
                     /locus_tag="JQH51_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY6077762.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="JQH51_003565"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="JQH51_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY6077763.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="JQH51_003566"
     CDS             7998..8171
                     /locus_tag="JQH51_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY6077764.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="JQH51_003567"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="JQH51_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY6077765.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DADLEE010000129         9763 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000129 DADLEE010000000
VERSION     DADLEE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.129"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            383..1501
                     /locus_tag="JQH51_003568"
     CDS             383..1501
                     /locus_tag="JQH51_003568"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY6077766.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="JQH51_003569"
     CDS             1567..1815
                     /locus_tag="JQH51_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY6077767.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="JQH51_003570"
     CDS             1880..2248
                     /locus_tag="JQH51_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY6077768.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="JQH51_003571"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="JQH51_003571"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY6077769.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /locus_tag="JQH51_003572"
     CDS             3103..4350
                     /locus_tag="JQH51_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY6077770.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JQH51_003573"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JQH51_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY6077771.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JQH51_003574"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="JQH51_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY6077772.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="JQH51_003575"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="JQH51_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2389 a         2705 c         2368 g         2301 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccagattaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg cag
//
LOCUS       DADLEE010000130         9683 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000130 DADLEE010000000
VERSION     DADLEE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.131"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(26..214)
                     /locus_tag="JQH51_003576"
     CDS             complement(26..214)
                     /locus_tag="JQH51_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6077773.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="JQH51_003577"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="JQH51_003577"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY6077774.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="JQH51_003578"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="JQH51_003578"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY6077775.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /locus_tag="JQH51_003579"
     CDS             3536..4270
                     /locus_tag="JQH51_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY6077776.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="JQH51_003580"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="JQH51_003580"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY6077777.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="JQH51_003581"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="JQH51_003581"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY6077778.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /gene="blaEC"
                     /locus_tag="JQH51_003582"
     CDS             5109..6242
                     /gene="blaEC"
                     /locus_tag="JQH51_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY6077779.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /locus_tag="JQH51_003583"
     CDS             6331..6864
                     /locus_tag="JQH51_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY6077780.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /locus_tag="JQH51_003584"
     CDS             complement(6861..7178)
                     /locus_tag="JQH51_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY6077781.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="JQH51_003585"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="JQH51_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY6077782.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="JQH51_003586"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="JQH51_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY6077783.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="JQH51_003587"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="JQH51_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY6077784.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="JQH51_003588"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="JQH51_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY6077785.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2481 c         2466 g         2271 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tct
//
LOCUS       DADLEE010000131         9595 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000131 DADLEE010000000
VERSION     DADLEE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.132"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JQH51_003589"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JQH51_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY6077786.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="JQH51_003590"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="JQH51_003590"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY6077787.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="JQH51_003591"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="JQH51_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY6077788.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="JQH51_003592"
     CDS             complement(2404..2607)
                     /locus_tag="JQH51_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077789.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /locus_tag="JQH51_003593"
     CDS             2685..3779
                     /locus_tag="JQH51_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY6077790.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="JQH51_003594"
     CDS             complement(3803..4015)
                     /locus_tag="JQH51_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY6077791.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="JQH51_003595"
     CDS             4275..4583
                     /locus_tag="JQH51_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY6077792.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="JQH51_003596"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="JQH51_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY6077793.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="JQH51_003597"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="JQH51_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY6077794.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="JQH51_003598"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="JQH51_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY6077795.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="JQH51_003599"
     CDS             complement(8479..8982)
                     /locus_tag="JQH51_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6077796.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9595)
                     /locus_tag="JQH51_003600"
     CDS             complement(9070..>9595)
                     /locus_tag="JQH51_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6077797.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DADLEE010000132         9542 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000132 DADLEE010000000
VERSION     DADLEE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.133"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..613)
                     /locus_tag="JQH51_003601"
     CDS             complement(<1..613)
                     /locus_tag="JQH51_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6077798.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(680..1405)
                     /gene="fimC"
                     /locus_tag="JQH51_003602"
     CDS             complement(680..1405)
                     /gene="fimC"
                     /locus_tag="JQH51_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY6077799.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1442..1981)
                     /locus_tag="JQH51_003603"
     CDS             complement(1442..1981)
                     /locus_tag="JQH51_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6077800.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2046..2594)
                     /locus_tag="JQH51_003604"
     CDS             complement(2046..2594)
                     /locus_tag="JQH51_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6077801.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="JQH51_003605"
     CDS             complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="JQH51_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY6077802.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="JQH51_003606"
                     /pseudo
     CDS             complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="JQH51_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            6205..6921
                     /gene="nanC"
                     /locus_tag="JQH51_003607"
     CDS             6205..6921
                     /gene="nanC"
                     /locus_tag="JQH51_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY6077803.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6941..8047
                     /locus_tag="JQH51_003608"
     CDS             6941..8047
                     /locus_tag="JQH51_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY6077804.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8112..9092
                     /locus_tag="JQH51_003609"
     CDS             8112..9092
                     /locus_tag="JQH51_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY6077805.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2793 a         2081 c         2001 g         2667 t
ORIGIN      
        1 aagttggcag cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
       61 cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg cgattactca
      121 taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag
      181 cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg
      241 cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa
      301 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
      361 tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
      421 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg
      481 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac
      541 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
      601 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
      661 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
      721 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
      781 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
      841 ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa
      901 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
      961 gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca
     1021 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt
     1081 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
     1141 gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
     1201 ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
     1261 ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca
     1321 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
     1381 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca
     1441 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
     1501 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
     1561 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta
     1621 caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac
     1681 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
     1741 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac
     1801 tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt
     1861 ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat
     1921 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
     1981 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg
     2041 gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc
     2101 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt
     2161 gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc
     2221 tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta
     2281 cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta
     2341 tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc
     2401 aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact
     2461 gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta
     2521 gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga
     2581 gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc
     2641 gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca
     2701 aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt
     2761 tcagtttatt tgttggctta atattctatt attatcttta tttatagatt tttatattgc
     2821 atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct
     2881 ttacatatag cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat
     2941 agttagatgc tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta
     3001 tttatgatat ggacagtttg gccccaattg tcttgtgttt atttgttttt taactttatt
     3061 atcaataagt taaatcaaac ctcttctctt tttaattttt cgtttatgag attatttctt
     3121 tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata
     3181 tttcgatgcc cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca
     3241 taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata
     3301 ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa
     3361 ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt
     3421 tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt
     3481 cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt
     3541 tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc
     3601 gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga
     3661 cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa
     3721 acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg
     3781 gctatcagtc aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat
     3841 ctattttcat tgatttgatt caatataaca atagattaca gaggattaac ttccagttac
     3901 ttaaaaaata aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt aaacttatga
     3961 atgattttgt ttatttattt tgaagaacat aaatcattag attttgtatg ggtatggtta
     4021 acttaatgtt tattatgata attaagaatc acacttacaa taacatcaat taataaatgg
     4081 ttaatcattt ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc
     4141 aactatctct ataaaacagc atgacgctgt cgtcctctgg ctctatccca gatgccgtaa
     4201 aaacgccctg cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga
     4261 taatcctaga taagtcgcgt atctattccc atattcgcca aagcaaaacc acacgaatag
     4321 cgtaacatgt gcggatgaat ctccagtgac aacccggcat taccaccaga agtcgagata
     4381 atatggtaaa actgttgccg agaaagcgga ttccccttac gtgataaaaa tacccactcg
     4441 ctctcagcat gcggataaga agtacggata ctcaaccagt tttttaaagc ctgaacttct
     4501 ttgttcaata gcgggtgcgt tgttgaaaag ccttttttta atcgatggat atatatacac
     4561 tttgctttaa gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca
     4621 tgaataaaac aaagcaaaat cagacaataa ttacgtgttg catgaggccc ggtatttgct
     4681 gctttaagga gtgactcgat ttcactatgg gtaaggaagt tccttttttt gttatcagcc
     4741 ttattcctca tcgttttccc ttataattac agacgcgcac tagctgtgct gggttagcaa
     4801 taatccaaca tttttatcct gattatgttt ataaaagcga acgtttgctt aataacttac
     4861 gtaagtggac cagttcttct gagcgaactt aaatggagta gcgatagtta cttataataa
     4921 ctttgccata caaacgccat gctcttgcat gctatgtacc tttatatatt tatcaatctg
     4981 atacgaatct aataataacc ctcatcatta atgcaactaa tcttatctat atatcatgtg
     5041 atatgttctg taacaagtaa aatcaacata aaaaattcag caaaatttaa tatttgttaa
     5101 actagccgtc tcaaatatga cattattagt taactgcatt tgccacaaaa tggctatttc
     5161 atggaacgca gtaatttctt agtggctatt atcatgctaa ttcttagatc cctagcgatt
     5221 attcctgcct ggttcgtcag cttaggccat gagattgtta cattcctttt tatcctggag
     5281 atatggggga acgcaggcga cgctttttca tctacatccg ttggtaaata tagctttggt
     5341 cactacgact taagccttcc tcaatcacag ctattcgccg ggcgatacgg caaacaaact
     5401 aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagatc gacatcagga
     5461 gtgaccagtt acaagccaca aaccaagaaa catatttcat gatgagaatt atgctcagcc
     5521 tgatggcgag taaaccctta ttttcaccac tctcactgag ttagcttgca aagctttctt
     5581 tgtttagatt ctaaataaat tatttataaa acgtatagtt aaaagaaaat ctttagtttt
     5641 tgaagaccac cgcaagtgtt cgtctggctt cacatggcat cttcttctta gaaaaagatc
     5701 gacatatttt gtgacgcgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg
     5761 atctggtata acaggtataa agatgcacca cgatagtcaa tgagactggg catctcgtaa
     5821 tctatggcaa acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg
     5881 aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatgggt
     5941 tgagtaagtg atataactta cgaatattca aatcaattaa aaatcagaag agattttata
     6001 ctcaggtatt taatctggat ctctgtttat ttaaataatg ttaaaagaga attttcacag
     6061 gagaccttat acaaaaaata taaaatacag ttacccgttg ccaaagacac tataagcctg
     6121 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
     6181 gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
     6241 ctgtgctttt cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat
     6301 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
     6361 tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
     6421 gccgcactca atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg
     6481 acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac
     6541 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
     6601 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
     6661 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
     6721 ctatacagca aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat
     6781 gcctttgttc tacaatacca tatgacgccc gatattacgc catacataga atatgactac
     6841 cttgaccgcc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
     6901 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc
     6961 gcttgctatc atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
     7021 gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
     7081 cgcaggtaca gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt
     7141 agctgcattc cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct
     7201 gtatgtgttt ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt
     7261 acacaaatac aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg
     7321 catggcgggt catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     7381 ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc
     7441 tatagataaa atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa
     7501 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg
     7561 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     7621 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
     7681 tttaaaatgg aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc
     7741 ggggataagc aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga
     7801 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
     7861 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     7921 ctacggagta tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg
     7981 cggcaaagcg gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca
     8041 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac
     8101 gcgaaaatta tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg
     8161 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     8221 ctcattccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat
     8281 gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     8341 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     8401 tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc
     8461 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     8521 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag
     8581 tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct
     8641 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     8701 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     8761 ttactgacgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc
     8821 attcgtatga agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     8881 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     8941 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     9001 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag
     9061 aagcaatttt gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc
     9121 ttgttcgttc tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact
     9181 tgcgggctcg aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc
     9241 gcaccgcata aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc
     9301 gccttttcga ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg
     9361 ggccacagcg ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat
     9421 tctggcaaat agacgatgat gatatgctta atgatgttca aagaaattag gccattatta
     9481 tattattgct tgtattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt
     9541 at
//
LOCUS       DADLEE010000133         9537 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000133 DADLEE010000000
VERSION     DADLEE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.134"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="JQH51_003610"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="JQH51_003610"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY6077806.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="JQH51_003611"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="JQH51_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY6077807.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="JQH51_003612"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="JQH51_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /locus_tag="JQH51_003613"
     CDS             4131..4886
                     /locus_tag="JQH51_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY6077808.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="JQH51_003614"
     tRNA            4965..5038
                     /locus_tag="JQH51_003614"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="JQH51_003615"
     CDS             5173..6783
                     /locus_tag="JQH51_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6077809.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6902..7234
                     /locus_tag="JQH51_003616"
     CDS             6902..7234
                     /locus_tag="JQH51_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077810.1"
                     /translation="MASEDWQREHHCRTGIIFTAFAIEAMFIFYRKQVDPGYDKTQKE
                     CRKTMHKNTLKLCGINNYMGTKPYQIIKECLEVRDAIAHGDSYTSSFNFSADHLDNQD
                     DIAKVSWR"
     gene            8353..9099
                     /locus_tag="JQH51_003617"
     CDS             8353..9099
                     /locus_tag="JQH51_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY6077811.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DADLEE010000134         9493 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000134 DADLEE010000000
VERSION     DADLEE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.135"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..190)
                     /locus_tag="JQH51_003618"
     CDS             complement(<1..190)
                     /locus_tag="JQH51_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077812.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="JQH51_003619"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="JQH51_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY6077813.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="JQH51_003620"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="JQH51_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY6077814.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="JQH51_003621"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="JQH51_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY6077815.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="JQH51_003622"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="JQH51_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="JQH51_003623"
     CDS             complement(3262..3381)
                     /locus_tag="JQH51_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY6077816.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="JQH51_003624"
     CDS             complement(3342..3527)
                     /locus_tag="JQH51_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077817.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="JQH51_003625"
     CDS             3628..3801
                     /locus_tag="JQH51_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077818.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3863..4147
                     /locus_tag="JQH51_003626"
     CDS             3863..4147
                     /locus_tag="JQH51_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY6077819.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVSAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="JQH51_003627"
     CDS             4151..4486
                     /locus_tag="JQH51_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY6077820.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="JQH51_003628"
     CDS             complement(4543..6864)
                     /locus_tag="JQH51_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6077821.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="JQH51_003629"
     CDS             complement(6981..7190)
                     /locus_tag="JQH51_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077822.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7609..7845)
                     /locus_tag="JQH51_003630"
     CDS             complement(7609..7845)
                     /locus_tag="JQH51_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077823.1"
                     /translation="MFESFDRIAISSMNNSNNLDYFTLYIIFSIAFMLITLLVILIAK
                     PSTGLGEVLVTINLLNALVWLAINLVNRFKRKTR"
     gene            complement(7941..9464)
                     /locus_tag="JQH51_003631"
     CDS             complement(7941..9464)
                     /locus_tag="JQH51_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY6077824.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DADLEE010000135         9440 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000135 DADLEE010000000
VERSION     DADLEE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.136"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            21..1154
                     /locus_tag="JQH51_003632"
     CDS             21..1154
                     /locus_tag="JQH51_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077825.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="JQH51_003633"
     CDS             1833..2051
                     /locus_tag="JQH51_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY6077826.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="JQH51_003634"
     CDS             2269..2511
                     /locus_tag="JQH51_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077827.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="JQH51_003635"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="JQH51_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY6077828.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="JQH51_003636"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="JQH51_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY6077829.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdxH"
                     /locus_tag="JQH51_003637"
     CDS             complement(4399..5301)
                     /gene="fdxH"
                     /locus_tag="JQH51_003637"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY6077830.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="JQH51_003638"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="JQH51_003638"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY6077831.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="JQH51_003639"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="JQH51_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY6077832.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2312 a         2315 c         2445 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat attttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DADLEE010000136         9412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000136 DADLEE010000000
VERSION     DADLEE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.137"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            70..222
                     /locus_tag="JQH51_003640"
     CDS             70..222
                     /locus_tag="JQH51_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6077833.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /locus_tag="JQH51_003641"
     CDS             573..725
                     /locus_tag="JQH51_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6077834.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="JQH51_003642"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="JQH51_003642"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY6077835.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="JQH51_003643"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="JQH51_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY6077836.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="JQH51_003644"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="JQH51_003644"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY6077837.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /locus_tag="JQH51_003645"
     CDS             5330..5548
                     /locus_tag="JQH51_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY6077838.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="JQH51_003646"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="JQH51_003646"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY6077839.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2288 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DADLEE010000137         9191 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000137 DADLEE010000000
VERSION     DADLEE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.138"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..904)
                     /locus_tag="JQH51_003647"
     CDS             complement(<1..904)
                     /locus_tag="JQH51_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY6077840.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="JQH51_003648"
     CDS             964..1242
                     /locus_tag="JQH51_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077841.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQH51_003649"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQH51_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY6077842.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQH51_003650"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQH51_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY6077843.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="JQH51_003651"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="JQH51_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY6077844.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="JQH51_003652"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="JQH51_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY6077845.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..4880
                     /locus_tag="JQH51_003653"
                     /pseudo
     CDS             4234..4880
                     /locus_tag="JQH51_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4922..5452)
                     /locus_tag="JQH51_003654"
     CDS             complement(4922..5452)
                     /locus_tag="JQH51_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6077846.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5608..6174)
                     /gene="slp"
                     /locus_tag="JQH51_003655"
     CDS             complement(5608..6174)
                     /gene="slp"
                     /locus_tag="JQH51_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY6077847.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6421..7644)
                     /locus_tag="JQH51_003656"
     CDS             complement(6421..7644)
                     /locus_tag="JQH51_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077848.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8225..>9191)
                     /locus_tag="JQH51_003657"
     CDS             complement(8225..>9191)
                     /locus_tag="JQH51_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY6077849.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2682 a         1826 c         1922 g         2761 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggttac gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg
     4381 aaatatggtt ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt
     4441 gctgctcagg tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac
     4501 caaaatattg taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg
     4561 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     4621 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     4681 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     4741 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     4801 ttcatgccac aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     4861 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     4921 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     4981 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     5041 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     5101 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     5161 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     5221 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     5281 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     5341 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     5401 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     5461 cctggtgcgg acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt
     5521 ataataataa tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg
     5581 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
     5641 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
     5701 atcccacagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
     5761 catattcact tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg
     5821 aatggtaccg aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct
     5881 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc
     5941 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
     6001 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
     6061 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
     6121 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
     6181 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
     6241 tgaggtgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag
     6301 ttcaattccg ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt
     6361 gttttaaaat tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata
     6421 ttatggtatt atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc
     6481 tatttccacc ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg
     6541 gtatgaaatc ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga
     6601 cattgttgcc catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg
     6661 tttataacat atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt
     6721 cactaccttt gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa
     6781 agagttgtca ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg
     6841 ttgttcatat ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa
     6901 taaatttgcg tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt
     6961 taggactagc ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc
     7021 aagggcttcc atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa
     7081 gtacaagctt tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt
     7141 tttattatcg gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag
     7201 aattttagtt atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac
     7261 agaatcctta tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg
     7321 ttgcgtaagt ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac
     7381 cacaactttt gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg
     7441 ttggagttgt tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt
     7501 ggaattaatg ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt
     7561 tttaacatta tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat
     7621 acctggggta aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt
     7681 ttttaaattg gataattaga acaggaatgg attttcttct acacacaata aatataatgc
     7741 gttatatctt cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt
     7801 gtgtataaaa aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa
     7861 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
     7921 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
     7981 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
     8041 taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc
     8101 cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta
     8161 tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat
     8221 ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg
     8281 tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca
     8341 ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg
     8401 tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga
     8461 caacggcgct taatgggatg gcgaagggat tatctgatcc tgcatgagcg gcaatccacg
     8521 ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca
     8581 gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta
     8641 ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac
     8701 tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac
     8761 aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca
     8821 ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt
     8881 tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa
     8941 ataaaaaagt gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg
     9001 gtattgtcga gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc
     9061 ctctcccttt tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta
     9121 tcaggctgac accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca
     9181 caaacatctc t
//
LOCUS       DADLEE010000138         9186 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000138 DADLEE010000000
VERSION     DADLEE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.139"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..923
                     /locus_tag="JQH51_003658"
     CDS             <1..923
                     /locus_tag="JQH51_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077850.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JQH51_003659"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JQH51_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY6077851.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQH51_003660"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQH51_003660"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY6077852.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JQH51_003661"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JQH51_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY6077853.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JQH51_003662"
     CDS             4446..4805
                     /locus_tag="JQH51_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY6077854.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JQH51_003663"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JQH51_003663"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY6077855.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="JQH51_003664"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="JQH51_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY6077856.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="JQH51_003665"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="JQH51_003665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY6077857.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2221 c         2483 g         2380 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgcagctg agaaaaagcg
     9181 aagcgg
//
LOCUS       DADLEE010000139         9113 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000139 DADLEE010000000
VERSION     DADLEE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.140"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQH51_003666"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQH51_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY6077858.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQH51_003667"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQH51_003667"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY6077859.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQH51_003668"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQH51_003668"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY6077860.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQH51_003669"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQH51_003669"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY6077861.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="JQH51_003670"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="JQH51_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY6077862.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="JQH51_003671"
     CDS             5562..6035
                     /locus_tag="JQH51_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY6077863.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="JQH51_003672"
     CDS             6064..6606
                     /locus_tag="JQH51_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY6077864.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="JQH51_003673"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="JQH51_003673"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY6077865.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="JQH51_003674"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="JQH51_003674"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY6077866.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="JQH51_003675"
     CDS             8003..8260
                     /locus_tag="JQH51_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY6077867.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="JQH51_003676"
     CDS             complement(8236..8790)
                     /locus_tag="JQH51_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY6077868.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2287 a         2212 c         2273 g         2341 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgc
//
LOCUS       DADLEE010000140         8811 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000140 DADLEE010000000
VERSION     DADLEE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.141"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..280
                     /locus_tag="JQH51_003677"
     CDS             <1..280
                     /locus_tag="JQH51_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HAY6077869.1"
                     /translation="RASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQAN
                     SIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
     gene            complement(306..983)
                     /locus_tag="JQH51_003678"
                     /pseudo
     CDS             complement(306..983)
                     /locus_tag="JQH51_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1070..1431)
                     /locus_tag="JQH51_003679"
                     /pseudo
     CDS             complement(1070..1431)
                     /locus_tag="JQH51_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1770..2324)
                     /locus_tag="JQH51_003680"
                     /pseudo
     CDS             complement(1770..2324)
                     /locus_tag="JQH51_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2467..2589
                     /locus_tag="JQH51_003681"
                     /pseudo
     CDS             2467..2589
                     /locus_tag="JQH51_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2851..3417)
                     /locus_tag="JQH51_003682"
     CDS             complement(2851..3417)
                     /locus_tag="JQH51_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077870.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3699..4013)
                     /locus_tag="JQH51_003683"
     CDS             complement(3699..4013)
                     /locus_tag="JQH51_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077871.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(4091..4201)
                     /locus_tag="JQH51_003684"
                     /pseudo
     CDS             complement(4091..4201)
                     /locus_tag="JQH51_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4525..5202)
                     /gene="ospD1"
                     /locus_tag="JQH51_003685"
     CDS             complement(4525..5202)
                     /gene="ospD1"
                     /locus_tag="JQH51_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY6077872.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5574..5711)
                     /locus_tag="JQH51_003686"
                     /pseudo
     CDS             complement(5574..5711)
                     /locus_tag="JQH51_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5793..6091)
                     /locus_tag="JQH51_003687"
                     /pseudo
     CDS             complement(5793..6091)
                     /locus_tag="JQH51_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6680..6839)
                     /locus_tag="JQH51_003688"
                     /pseudo
     CDS             complement(6680..6839)
                     /locus_tag="JQH51_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6843..8072)
                     /locus_tag="JQH51_003689"
                     /pseudo
     CDS             complement(6843..8072)
                     /locus_tag="JQH51_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8092..8439)
                     /gene="tnpB"
                     /locus_tag="JQH51_003690"
     CDS             complement(8092..8439)
                     /gene="tnpB"
                     /locus_tag="JQH51_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6077873.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(8439..>8811)
                     /locus_tag="JQH51_003691"
     CDS             complement(8439..>8811)
                     /locus_tag="JQH51_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6077874.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         2265 a         2069 c         1956 g         2521 t
ORIGIN      
        1 tcgggccagc aaaagtcgtt caacaggaag aattctggtt ctgagtctgc tggcaacgtt
       61 gagcactatc gtaatgtggt tactcggtta tcacgctgaa aataaagggt tacacctgaa
      121 atatcaggct aacagcatta aatcccggcg ggttatctca tatctgacgt tagcgaagaa
      181 tgttcttcga cactcgccac ttattttaag acgaacagta ctgagcacag ttctaaacca
      241 tctgtccaga acctaccgaa atatggtgct ggtttattag cagtaatttt gtggggatcc
      301 ctcagcctga aggggcgaca cagtcgggga gagaagccca tctccttgcc gaactgccac
      361 agcgaaggat ggaaccggtg ctgaccggtc tgatatgcgt cacgttgcag aaccacagtt
      421 ttcatattgt catacaacac ttcgcgcgac acaccaccaa agaagcggaa cgcattacga
      481 tggcaggtct ccagcgtgtc ataacgcata ttgtcagtga attcgatgta caacattcgg
      541 ctgtatccgg gaacagcaac gaacacgtga agcggtgagc gaccattacg catagtgccc
      601 cagtcaacct gcatctgtcg tccgggttca gtttcgaacc gaacggcagg ctcctgctcc
      661 tgaggaaccg agagagaacg aatgaatgcc ctgagaatgg tcattccgcc acgatatccc
      721 tggtctctga tctcgcgagc gattaccgtt gccgggattt tgtaaggatg agcatcggcg
      781 atgcgttgac gaatataatc ccggtattca tccaggagtg aagcaacagc aggtcgcggc
      841 gtatattttg gcggctcaga ttttgcctgc aaataacgtt taacggtatt gcgggagagc
      901 cccagttctc tggcaatcgc ccggctactc attccctgct tgtgcaggat tttaatttcc
      961 ataactgtct caaaagtgac cataagctct cctgaatcag gagagcagat taccccctgg
     1021 atctgatttc aggcgttggg tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg
     1081 ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca acatcagcgt
     1141 gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc gtatgacggt
     1201 attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt tgaaagccat
     1261 ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag tgagctgaaa
     1321 cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct gcccgtgacg
     1381 gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca tgaaagaacc
     1441 taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat ttttaatcta
     1501 tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt tagagttttt
     1561 tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa gaaaagataa
     1621 gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga cagatcactc
     1681 tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt
     1741 gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc aaattgttcc
     1801 ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca ttcgatataa
     1861 ttaaacactg ttgccctcat tatttcccgg ctgataaagt gttctccgtg gagacattcc
     1921 actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca gccctttgcg
     1981 ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc tgaacagtac
     2041 tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg ccacagcgcc
     2101 atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata
     2161 acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc atctgtacgt
     2221 aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc
     2281 agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt gctgggtaag
     2341 atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga gccgtatgac
     2401 gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct ccggctgact
     2461 cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc gctgatcggc
     2521 acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat tgaacatatc
     2581 caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga tctgacttct
     2641 cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa aggggagagg
     2701 gatcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat tcaatatatt
     2761 aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt atttactttt
     2821 cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat ttgtaatata
     2881 gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt tctcaaaaaa
     2941 ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc ttctattcat
     3001 cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata caagatgatt
     3061 tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct caacctgttt
     3121 aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac aaaacttatc
     3181 aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt tccaatctgt
     3241 aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta ttttggttga
     3301 tgatattttt actatccttt tccccattgt acagcggata atctcatcca cctttcctga
     3361 aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc caagcattat
     3421 aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc aaacaattct
     3481 gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg aatcccagag
     3541 actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa atgaagggac
     3601 caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg tagttatgaa
     3661 gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc atcagctgag
     3721 gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt catgaaaact
     3781 ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc actgttgctg
     3841 ataaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat aatgaaacaa
     3901 cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc aatcgcaaca
     3961 aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag catgaagtga
     4021 tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc cacgtaatat
     4081 gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga cctccacacc
     4141 aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact gtatatttca
     4201 tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac tatgatgcca
     4261 gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag tgcactttac
     4321 ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt ttttgcgtcc
     4381 agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct agttaactgt
     4441 ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct tctaagtgca
     4501 attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag aaataatatt
     4561 aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt ttatcccact
     4621 atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc ctgcggccag
     4681 aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg tttcttttgg
     4741 tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg aatgaccttc
     4801 ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa gaacttctag
     4861 tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac gcgctgcatc
     4921 ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct tattattctt
     4981 tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac cactatatcc
     5041 cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca cgttaatgat
     5101 attatttttc attcctttat tttcattagc agtattgctg cctattatta agtgcatatt
     5161 tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc atcctttttg
     5221 ctaaaaaaaa acaataacct atttttgtaa tgtaatagat attagtctat tgctaatcta
     5281 aaaaaaccat tgccacattg aatggctata ttcaccaata aattctttcg atttcgtata
     5341 ttgacatatc gtagatatat cttatcccta agattcaata atcgagtccg tttaccattc
     5401 tgtgcaagcg ccttcactca gaagtgaccg tcaaggcgat caccaaactt gataataaag
     5461 cagctcattg ccatacgcca gtccttaagg ggcgttgtcc atttctgtga tgctgcctag
     5521 atttaactgc cagctccggc ttatctacat ctaaatatgt gagcctaata ccgctacact
     5581 ttgtccagac tatgtttttc tccgggaagt gggctaaccg tctccataaa gtgacgaact
     5641 tttttcaaca gtggccatat tgaacggcac tgatgattac gggttattgt ctcatgaaga
     5701 gccggccaca tcactgacag cagtgacttc aggccttcag caccagacag cctgaaccag
     5761 ttaatccatc gcccgacaaa tttagtaatc tagggctggt ttttcttcag ctgttgcggt
     5821 tcgaaagtgc cattacgatc acgcagagta cggatttcca gaggttcatc tccagtagtg
     5881 ataatcttcg ttaagcaacc attgcgggaa ttgctgccag atctaagctg atttttctcg
     5941 cacccaagat gatgagtcat attagcattg agtacggttt cattttcagc atccaatcaa
     6001 actgactgag atcttcagga tttttgagac ttttggctag ttcattagct aaagcccgca
     6061 acttattaga tgtcaactgt ttttcattca taaattcacc ttcatttgat atcaaattaa
     6121 atagttcaac atcagagaat tatacaaact tatgtacagg ctcctatgat cttcgaatcc
     6181 tgaagattat agtgaaaata agttttcacg ttgaaatccc acatagttag tatatgataa
     6241 ataatcatgg atgacctgat taacctcttg gggtagtgct tttatagaag aataaatatt
     6301 ctactaattt aatacatcac ctaaaatagt attgtttttt atggttggtt gaaggtcgtg
     6361 gtgatgatgt tagtcctttt gagaatattc cagtgccaga tcttacttat aataaagggg
     6421 cttggcagtt tggataaact attgttctta ttccactaat acaaactttt tgtaaagtat
     6481 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
     6541 ataaaaggaa gagggggccc taattaagtt gtcaagcatg tttatgatac ctgcagggta
     6601 taaattttct ggctgcaagc agacacatat aaaacgtctt tattgatcct acccacataa
     6661 tatggacaca gtcgtaagtg aggctctggt tttcaaattg ttccggactg agaccaccac
     6721 accaacggtg ccgtcgccag cgattgtaat cgcactcgat ataactaaac actgttgccc
     6781 gcactatttc ccgatggata atagtgttct ccgtggagac attccatttt cagcgaatga
     6841 attttcatct gttgctgtat ccagtcatac agtgactgca tcagtggcgc ggctctggct
     6901 tttcttgccg ccagacgctg ttctgctgaa catccccgga cttctgcctc gatggcatac
     6961 agttcaccga tacgctgcag ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca
     7021 tcgtggattt ttctccggac atgagccata catgcggctt ccgttattct gccggattcg
     7081 tacaacgccc ggtaaccacc gtaagcatcg gcctgaagca ctccgctgta accggccaga
     7141 tgattctgtg gatggatacc tttccggtcc ggtgagtacg cgaaccagac cgccggaggc
     7201 atttccgaac cggcgttacg gtcatcacga acgtagaccc acagccgggc agtccttgtt
     7261 ttaccgctgc ccggctcctg gaccggaacg gggatatcat cggcatggac tttaccgggc
     7321 atcagcacat actggcgcag gatgtcatac agcggttcca gcagttcagc aacagcacct
     7381 gtccagcgcc ccagtgtggc acggctcagc tccacgccct gacgacggta tatttctgac
     7441 tggcggtata acggcagata gtctgcatat tttccagcaa caacatgggc cagaagcccc
     7501 gctccggcat aactgcgtgc aatgggtttt gaaggcaccg gtgcctgcac gatatggtca
     7561 caccggcaac aggccagttt cggacgttgt gtttcgataa ccttaaaggc gctgctgata
     7621 agctccagct gctctgacac atcacatccc agagaactga gttcgccacc acaggccggg
     7681 cagcattcct cttccggacg gatgacccgg gtttcacggg gaagtgaggc tggtaacggt
     7741 ttacgggctg aagactggcg caggggagat ggcagtaccg ggtcatattg ctcacccagc
     7801 gtttccgcca tttcttcctg aagtgcgctg attcgctcct gtgcttccag tatctgccgt
     7861 tcggtttttg cgcgaagttt ttctgagctt ttaccgaact gcatgcgctg cagtttcgca
     7921 accagcgcct tcagccggtt gatttcggaa gcataagccg ccacccgctg tgagagcagg
     7981 cggttgtatt tcgccatctg gcggatggtg tcctgttgcg tctgcaacag tgcccgcagg
     8041 cgggcattct catgagcaag tgaggtgtcc atatcctcac tttacaacgg gttatatgcg
     8101 gatgccagcg cgttccgttc gtttcgggtg tttccagtcg atgccctcaa gaagcatgga
     8161 taactgggcc ggagtaaggt gcaccttgcc gtcacgggtg accggccaga cgaagcggcc
     8221 ccgctccagg cgtctggtaa agaggcacag tccgtcactg tcagcccaca acacttttat
     8281 ctggtcaccc cggcgtccgc ggaagatgaa cagatgcccg gagaacgggt catccttcag
     8341 gacgttctga acttttgaga ccaggccgtt aaagccattt cgcatatcgg tgatacctgc
     8401 aaccagccag atacgcgaac ctgcagggaa agatatcatc agtggctgct cccttttatt
     8461 tcgcggataa gtatctgtaa taacgccggc gtcagtttac ctttaagcct gagagttccg
     8521 gccggcagaa ccagctcaca gcacagactg tcggacggtg tatttatctg ctctggttcc
     8581 tgtgccggag ccgggatttt attatccggc tccggcgtta acgtcacggg aagcagtgca
     8641 ggcatatttt ttccggaagg cagcaggcca cctttccggt attgatggcg ccagttgaag
     8701 agcaggttat cgttgattcc gttttcacgg gcgatctgcg ccacacaggc tccgggctgc
     8761 agtgactgct ccactaaagc gattttaaat tcataaggaa aattaggccg c
//
LOCUS       DADLEE010000141         8760 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000141 DADLEE010000000
VERSION     DADLEE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.144"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            8..439
                     /locus_tag="JQH51_003692"
     CDS             8..439
                     /locus_tag="JQH51_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077875.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="JQH51_003693"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="JQH51_003693"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY6077876.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="JQH51_003694"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="JQH51_003694"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY6077877.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="JQH51_003695"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="JQH51_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY6077878.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="JQH51_003696"
     CDS             complement(4441..5148)
                     /locus_tag="JQH51_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY6077879.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="JQH51_003697"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="JQH51_003697"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY6077880.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="JQH51_003698"
     CDS             complement(6006..6209)
                     /locus_tag="JQH51_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY6077881.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6398..7549
                     /locus_tag="JQH51_003699"
     CDS             6398..7549
                     /locus_tag="JQH51_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077882.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYEGAQAWYPHYS
                     WHSDGRNILYAGEVVQNPPGTPPVDVASFKAWGDFAADKHSLYFEGKRTDDNGGGNSL
                     DIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPESFRVLAQKSWDQRGKFST
                     TFNPCIAVPFGPWDTLARTRTKILLNGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQFQPISDAVAQ
                     HQDSLYTIIETESGNRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEG"
     gene            7571..7876
                     /locus_tag="JQH51_003700"
     CDS             7571..7876
                     /locus_tag="JQH51_003700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077883.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            7873..>8760
                     /locus_tag="JQH51_003701"
     CDS             7873..>8760
                     /locus_tag="JQH51_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY6077884.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2388 c         2251 g         2089 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DADLEE010000142         8679 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000142 DADLEE010000000
VERSION     DADLEE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.145"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="JQH51_003702"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="JQH51_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY6077885.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="JQH51_003703"
     CDS             complement(2012..3217)
                     /gene="hemX"
                     /locus_tag="JQH51_003703"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY6077886.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="JQH51_003704"
     CDS             complement(3239..3979)
                     /gene="hemD"
                     /locus_tag="JQH51_003704"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY6077887.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="JQH51_003705"
     CDS             complement(3976..4917)
                     /gene="hemC"
                     /locus_tag="JQH51_003705"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY6077888.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5304..7850
                     /gene="cyaA"
                     /locus_tag="JQH51_003706"
     CDS             5304..7850
                     /gene="cyaA"
                     /locus_tag="JQH51_003706"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY6077889.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="JQH51_003707"
     CDS             complement(7890..8210)
                     /gene="cyaY"
                     /locus_tag="JQH51_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY6077890.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8258..>8679)
                     /locus_tag="JQH51_003708"
     CDS             complement(8258..>8679)
                     /locus_tag="JQH51_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY6077891.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1975 a         2240 c         2235 g         2229 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt ttagcttccg
     2101 ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc agtttttcca
     2161 gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc tggcttaact
     2221 ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag taagcacgta
     2281 cccaggtgga gacgttctcc agcgcctggc gataagtctc ttcctgatga cgcggtacag
     2341 cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata tcctgatttg
     2401 gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag ttgtccataa
     2461 agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg gaaagctctt
     2521 caccgtctga atccatcggc gaaccatcgc tgtcattatc ggccagacgc aggttatcta
     2581 cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc tgcgatactg
     2641 cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga ctcgggtcat
     2701 tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagaggtc gtgacgtcct
     2761 gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga gccagcagcc
     2821 aggttttagc atcgctgccg gaaatggtgg cgatcttttg ttggacttca tccaactgtt
     2881 ttgccagcgt ttcttgctga cgattcgcct gcttaagctg cgcagcttgt tgcttaataa
     2941 tgccttccag ctcggctttt tggctctcct gggctttttg caatgccgtc agttggttag
     3001 ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag ccatacaaac
     3061 cgacacccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat gcagtattgt
     3121 tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc tccctggtct
     3181 cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt tatgagagtt
     3241 attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta atgtcttgcc
     3301 agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt cgacagtgta
     3361 gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     3421 cgctggtaac aacgaccatc gtcacctcgc gggattgcca gcgcatcgct tcttctgcac
     3481 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgca
     3541 ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     3601 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     3661 gtgggtagag aatcttctgt ccgcttacgg tatgtagcgc cagtgcggtg gtgcgtccaa
     3721 tggcgaaata agtaggtagt tggggccatt tacgatcttg ctgatgcagc tgtgattggg
     3781 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc agcgctgcca
     3841 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3901 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg ggagacgggc
     3961 gggtgaccag gatactcatg ccggggcctc tccgttatag acttcagcga ggatctcgcg
     4021 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     4081 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccagcgcaca
     4141 tagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     4201 ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt cgtggtgatt
     4261 cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa taccgactgc
     4321 accttgtcct accgccggaa gagaaatctc gggtggtaac gcggcgcgaa tgcgggactc
     4381 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt cgccgttatc
     4441 tagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     4501 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgctgg
     4561 taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat cttcacgctc
     4621 gcaaatagtg accagcccca gaccttgcgg gaattcaacc ggcacatctt tcattgaatg
     4681 tacggcgata tcagcgcgat tctccaggag cgcgacttcc agctctttta caaataagcc
     4741 ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     4801 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     4861 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4921 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg atgtcagtgt
     4981 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     5041 aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     5101 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     5161 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     5221 tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac agtcatgacg
     5281 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     5341 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     5401 aggtctacag tctactgccg acattgttgc actatcacca tccgcgaatg ccgggttacc
     5461 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     5521 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggatccaccg aaaggtgagc
     5581 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     5641 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttac
     5701 tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     5761 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     5821 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtact gccgtgcgtc
     5881 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5941 actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg ctggatctcg
     6001 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     6061 agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa gcctattcct
     6121 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     6181 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgtgtt actgaatacc
     6241 tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc tatttaaaag
     6301 tgtgcgaaaa actcagccgt gaacgcgcct gtgtaggctg gcgtcgcgca gtgttgagcc
     6361 agttagtgag cgagtggggt tgggacgaag ctcgtctggc aatgctcgat aaccgtgcta
     6421 actggaagat tgatcaggtg cgtgaggcgc acaacgagct gctcgacgcg atgatgcaga
     6481 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagtccgc
     6541 aggatatcgg cgtactgacg cgtaaactgt atgccgcgtt tgaagcattg ccaggtaaag
     6601 tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg acctttattt
     6661 atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc gcgccaaata
     6721 ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     6781 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtatatt aaaggtaacg
     6841 gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac catttcccgc
     6901 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatt cgccatctgg
     6961 cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag gtggtgcatt
     7021 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtaggta
     7081 gcgttgacct gctgtaccgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggcg
     7141 agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag gacgccgcac
     7201 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta atccgtactc
     7261 gcgtgcagca actggtttct gagtgtattg aactgcgcct ttccagcacc cgccaggaaa
     7321 ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc ttcgaacgcc
     7381 tgaatgtatc ggtacagaag ctggagaacg ccatcgagtt ttatggcgct atttcgcata
     7441 acaaactgca cggcttgtca gtgcaggttg aaaccaatca cgtcaaatta ccggcggtgg
     7501 tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg caagacgaga
     7561 atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat caccactgcg
     7621 aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca tcgcacgacc
     7681 gcttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat cagattgtga
     7741 aggttgatgg tcgcgaacag gtgattccgt tccgaacaaa atcgattggc aacctgccgc
     7801 ccgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga tgagcgtgcc
     7861 ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc acccgcctgt
     7921 tgcgtcgccg cctgttccag caaatcccag aaggtttcgc cgctgcgatc gcaaatccat
     7981 tcatcgcctt tcaggtcaaa atggtagccg ccctgtttgg ttgccagcca cacctggtgc
     8041 agcggctcct ggcggttaat aatgattttg ctgccgtttt caaaggtaat ggtcagtacg
     8101 ccgccgttga tttcgcagtc gatatcgctg tcgccatccc tgtcgtccag acgctcttca
     8161 atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat tgttgtatcc
     8221 tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa gtttgcacct
     8281 ctgctgctgc gttccggcat gattcatccg tcaccggaat aatgatgtct ctgttttgaa
     8341 cttcacgtac gttctcctct ctgacacgag ttttcgccgg gcagggggag ggcgtaatcc
     8401 ttacctctgt atcggtccat gtataaacat tgcgatcgtc ataagcgaag cgatcgtttt
     8461 tagttagcga accgttaact ggaataattt gcaagctctt tgaccgtaat tggcggataa
     8521 agtaagcatg ctgtttgccg ctattaaatg agcgatcaat gaccaggtct ggcgtatcca
     8581 gcgggattat ctcaagctgg tcttcaccaa atgtcgataa ttttgttaca tacagccgat
     8641 ctttatattg cgcaatattg ccagtaatca gatggaatt
//
LOCUS       DADLEE010000143         8495 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000143 DADLEE010000000
VERSION     DADLEE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.146"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            44..397
                     /locus_tag="JQH51_003709"
     CDS             44..397
                     /locus_tag="JQH51_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY6077892.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQH51_003710"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQH51_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY6077893.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQH51_003711"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQH51_003711"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY6077894.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQH51_003712"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQH51_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY6077895.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQH51_003713"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQH51_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY6077896.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQH51_003714"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQH51_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY6077897.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQH51_003715"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQH51_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY6077898.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="JQH51_003716"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="JQH51_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY6077899.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /locus_tag="JQH51_003717"
     CDS             7001..7612
                     /locus_tag="JQH51_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY6077900.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8495
                     /locus_tag="JQH51_003718"
     CDS             7649..>8495
                     /locus_tag="JQH51_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708524.2"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY6077901.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIG"
BASE COUNT         1904 a         2306 c         2287 g         1998 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gctgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggca
//
LOCUS       DADLEE010000144         8441 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000144 DADLEE010000000
VERSION     DADLEE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.147"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="JQH51_003719"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="JQH51_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY6077902.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /locus_tag="JQH51_003720"
     CDS             309..620
                     /locus_tag="JQH51_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6077903.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="JQH51_003721"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="JQH51_003721"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY6077904.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /locus_tag="JQH51_003722"
     CDS             1190..2671
                     /locus_tag="JQH51_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY6077905.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="JQH51_003723"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="JQH51_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY6077906.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="JQH51_003724"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="JQH51_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY6077907.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="JQH51_003725"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="JQH51_003725"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY6077908.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2136 a         1999 c         2200 g         2106 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcctggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgttgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     8341 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     8401 acatccggca aaaacaacga gccaataaaa ataccggcgt t
//
LOCUS       DADLEE010000145         8374 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000145 DADLEE010000000
VERSION     DADLEE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.148"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..956
                     /locus_tag="JQH51_003726"
                     /pseudo
     CDS             1..956
                     /locus_tag="JQH51_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1128..1235
                     /locus_tag="JQH51_003727"
     CDS             1128..1235
                     /locus_tag="JQH51_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077909.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="JQH51_003728"
     CDS             complement(1288..2046)
                     /locus_tag="JQH51_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY6077910.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="JQH51_003729"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="JQH51_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY6077911.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="JQH51_003730"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="JQH51_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY6077912.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="JQH51_003731"
     CDS             3759..4619
                     /locus_tag="JQH51_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY6077913.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="JQH51_003732"
     CDS             complement(4660..5196)
                     /locus_tag="JQH51_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077914.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="JQH51_003733"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="JQH51_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY6077915.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="JQH51_003734"
     CDS             6174..6764
                     /locus_tag="JQH51_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY6077916.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /locus_tag="JQH51_003735"
                     /pseudo
     CDS             6897..8287
                     /locus_tag="JQH51_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
BASE COUNT         2171 a         2004 c         2009 g         2190 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccattatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctgggcc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact cata
//
LOCUS       DADLEE010000146         8333 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000146 DADLEE010000000
VERSION     DADLEE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.149"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..159
                     /locus_tag="JQH51_003736"
     CDS             <1..159
                     /locus_tag="JQH51_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY6077917.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="JQH51_003737"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="JQH51_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY6077918.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="JQH51_003738"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="JQH51_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY6077919.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="JQH51_003739"
     CDS             875..1246
                     /locus_tag="JQH51_003739"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY6077920.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="JQH51_003740"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="JQH51_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="JQH51_003741"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="JQH51_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY6077921.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="JQH51_003742"
     CDS             6949..7509
                     /locus_tag="JQH51_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY6077922.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="JQH51_003743"
     CDS             7640..7852
                     /locus_tag="JQH51_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY6077923.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8036..>8333
                     /locus_tag="JQH51_003744"
     CDS             8036..>8333
                     /locus_tag="JQH51_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY6077924.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLALFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca cat
//
LOCUS       DADLEE010000147         8280 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000147 DADLEE010000000
VERSION     DADLEE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.150"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            8..658
                     /locus_tag="JQH51_003745"
     CDS             8..658
                     /locus_tag="JQH51_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077925.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            630..1064
                     /locus_tag="JQH51_003746"
     CDS             630..1064
                     /locus_tag="JQH51_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6077926.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1837..2886)
                     /locus_tag="JQH51_003747"
     CDS             complement(1837..2886)
                     /locus_tag="JQH51_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY6077927.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3337..4527
                     /locus_tag="JQH51_003748"
     CDS             3337..4527
                     /locus_tag="JQH51_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HAY6077928.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4917..6377)
                     /locus_tag="JQH51_003749"
     CDS             complement(4917..6377)
                     /locus_tag="JQH51_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY6077929.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6374..7303)
                     /locus_tag="JQH51_003750"
     CDS             complement(6374..7303)
                     /locus_tag="JQH51_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY6077930.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7300..7662)
                     /locus_tag="JQH51_003751"
     CDS             complement(7300..7662)
                     /locus_tag="JQH51_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY6077931.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7924..>8280)
                     /locus_tag="JQH51_003752"
     CDS             complement(7924..>8280)
                     /locus_tag="JQH51_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6077932.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2531 a         1597 c         1686 g         2466 t
ORIGIN      
        1 gtaatccatg catcgtatag acacgaaaac cgcgaagaag gataagttcg gcgcgggtaa
       61 gaacggtttt acccgtggta acccccagac tggcacgcct gccaccgatc tggatgatga
      121 ctactttgac atgttgcagg aggagctttg cagcgtggtg gaggcatccg gtgccagcct
      181 ggagaagggg cggcacgatc agttgcttac cgcacttcgc gcgctgctgt taagccgcaa
      241 gaatccgttt ggcgatatca aatcggatgg cacggtgaaa acggctctcg aaaaccttgg
      301 tttgggagaa gcagctaaaa ggaatgtagg tacaggggcg aatcagatac ctgatatgag
      361 cctgttcgcg tcaattaata ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat
      421 tttacagtgc ggtcagttga atggtgcccc gaatgtatct tcaacatacg ggatgaggtt
      481 cccgatgacg ttctcaagag taattgctgt cgtagttaca ttgaacgtta ctggcgcggc
      541 tgggcagccg actgtatcgg cgacaagtgt ccagaacact ggatttaata ttactgtgtc
      601 gcccggttca ggatacggtt catctgctga tgcgtattac attgcaatgg gatattaaca
      661 aaatgtcata tttttattct gcatcgacaa acggatttta ttcgactgaa tttcacggca
      721 ccaatattcc tgatgatgca gtggaaatct cggaatcaga gtggaaaaca ctgattaatg
      781 cacagagtgt aacaaaaatg attacctgtg gtgagaacgg tcatcctgtc attgttgacc
      841 gtccttctcc aacaccagaa caattagcct taataaatga tgaaaagaaa tctgcactga
      901 tagcagaggc aacgaatgta atagctccgc ttcaggatgc ggttgattta ggtatggcaa
      961 cagatgatga aacgaaactg ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg
     1021 ttaatgtagt aaaacccgag tggcctatgc atccaaataa atagacttgt taatctagga
     1081 taattgttgg aataatactc gtttttcagt aggttcaggt agcctgggta taatgctcac
     1141 ttctacaatg atttcaactt attttattta attgatatta cgaaacttaa ctcatacata
     1201 aacaatatga ctcaatactg tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca
     1261 gcccttgctg ggcgaagcat caggaaggcg ttctcagcta ggttgtgtta tatctggctt
     1321 gagttttgtt gcccatacgc aacacattac ccatctgtaa acgacaagtg tggcagaatt
     1381 aggtgcagct atttagacta tatcgacgca gtaacgataa tagatatcga aactgtacta
     1441 gatattagct acttatggtt accgggggga gtcttttgag cactggtgtt atcaattggc
     1501 ttcaaataat tcgccaataa attatattgc gatagctcat caccttccag gttgtttata
     1561 gccagtttat cagcgtcacc acctactgtt tatgctttta tttctttgat taataagtat
     1621 atgatatttt aattcatctg gtgtatttct ttctcatgtg ttggtcgact gaatatctgt
     1681 tcagtgaagg ggtaatttta tgtttgcact tcgaacattc ccttcagttg ctaggaaaga
     1741 aaaccgtaac atttgcgcat taattttaac agtataatct gagcataaat atgcgctata
     1801 tatctataaa aaggcgcaat taattgcgcc tttttatcat tttatcaaaa atggcccacc
     1861 gactgtcctc gatagccaat ggccaatttt atctgataca tatacacaaa tacaggtata
     1921 tatgattgcg cagataggat ttggaatagt cacgctgaag cctataattg ctaaagccat
     1981 agggataaat aatttggtga tatactccat tccacatatg ttaagagtat ttcttccagc
     2041 tcttacaata aagtcatttc cacaaattat ttttgctatg aaaatattaa agattatcaa
     2101 gcctaccgtg gtgataatag tattaatagc ttgaatataa ctacttgata ttatgtctgc
     2161 attgaatatt gagataatct taaaaagtaa aggtttctgg ttaagtagtt gatacgcact
     2221 cattatagcg gtaattgaga agacaatgaa accaatttta gattttccaa aaaacctgtc
     2281 acgtgttaat tctaaaaaca tacatctacc gagaggcaat aaccaccagt atgccatagc
     2341 ggagtctatg ttccaaaacc attgaggatc tagtaatggg ttatgattga gcactgtttg
     2401 tgacaacatg aacgataaca gtgatatgat taaaatcact atattgttct tgactatttc
     2461 tctaaagatt gcatcaattg ctattataac aaaaaggcag tttataaacc atattgttcc
     2521 tacgaattgg ttatttctaa caccatatat tatatcataa atgtggttgt atatataatc
     2581 aatcgtctca cctgagttaa ttgtgtttat tattaatata gaaaatgcaa aagtgaaata
     2641 tggaatcatt aatctgtaaa actttgactt gatataatcg aaaacagata agtcattttt
     2701 tttgatagta aaaaaaccag cagcgaaaaa gaatagtggg acgtgataac taaaaacaaa
     2761 aggatacaat ttgccagccc ccaacccaag gtgacctaaa tatatagcaa aaataccgag
     2821 aaattttaaa gcatcaaccc aatcatgtct tatttttctg ttgctgtcga ttttgttatt
     2881 atacatttaa agtaggctct ccagttttgt gtgattttta aacactcatt gcaaatatga
     2941 atgcctgaaa gtttatgatt attaatcaac gagttcaata ttaatttatt atttttcaaa
     3001 gtcgcactat ataggcattt acttccgcag taccaccgat agtatcgata tcaaatcagc
     3061 ctgcggccgc gataactttc gtaacattct gccagcggct ttgttgaata aatcaggttc
     3121 aagataatcg tctctgcatc aggttagagt ttcaggcaat acatcctctt tctggcatac
     3181 ctgccactct cgcaccatgg tcatagcctt tacgatctgc atactcacca gaccagagcc
     3241 cgctcccttt taggctgagg aatccccaga aaagaagaca ggcatagagt tttgtgatct
     3301 actgattgcg ctaacaaatt caatgagatc acctctatgc ctgcccgtaa agtatgccag
     3361 acttttttcc gtaatgcttt agcttccacg catcagtatc gacaaaatgc cattattgat
     3421 tctgctgctg ctttagtcgg tggcgcatct ctttctctta ccagcattgg ccggcattta
     3481 cccggtcctg ctcgcgtgaa agataaaata aaacgcgttg actgtctgtt gggtaataaa
     3541 cgacttcata atgatatccc tctgatattc aaaaatatta catccatgat gacaaataaa
     3601 ctgtcgtggt gtgttatcgc tgttgactgg agcggttacc cttttcagga atatcatgtc
     3661 cttcgtgcca gcctcctctg cgacggtcgc tctatccccc tgatgagtca ggtcttcccg
     3721 tcaaaaaaaa ataacgaggc ggtggaaatc gcttttctgg atgcgctttc cggggccatt
     3781 tctccccgga ctcgtgttgt tattgttact gatgctggtt ttcaaagtgc atggttccgt
     3841 catatcaaat cacagggatg ggattttatt ggtcgtatca ggggggtagt gaagttcagg
     3901 ctggatagtg acaaagataa gtggctggac ataaaaatgt gcagggggag ttcagaggca
     3961 aaatacctgg ggacgggaac cctggcccgg aaaaaacgct cgcagtgtga aggacatttt
     4021 tatctttata aacattcacc taaaggcaga aaaagcaggc gtgccagagg gaggccgggc
     4081 ttaccaacaa cggaaaaaga acaaaaagcc gcaggcagag aaccatggtt aatattcagc
     4141 aatacagctg agtttaatgc caaaaaaatc atgaaactgt acagtcgaag aatgcagata
     4201 gagcaaaact ttcgggatga aaaaagtgaa cgctttggtt ttggccttcg ggcgagcaga
     4261 agtcggaggg gagagcgttt tctggtatta agtctactgg ttacactggc cagtatcgta
     4321 ctctggctac ttggttatca ttttgaaaat aaaggatttc atttaaagta tcaggctaat
     4381 agtctcaaaa aacgaagagt attgtctttt ctgacgctgg ctgaaaacgt cctgagattt
     4441 aaccctgaac ttctacgacg agcacaacct gaaaagatat tgtgccggtt ggccagcact
     4501 taccgaagta tggtgctggc ttactgatga aaattttctg gggatccctc agcctttcgg
     4561 ggaggaatga gggctctgat attcttttgc cgtagttcat cgtgaaaccg tcggatgtca
     4621 tttggcctga gtgacccatg cctcccaaaa gcagagtcgc tagtgttatg taattaggcg
     4681 gttaaacttc aattctcagt gttaagtttg ggtaaacgct tgtaaatggt attgctaatt
     4741 ttttaattga ctaatattag aaacggttag tgtgattgta tatagatatt tgagtgtaag
     4801 gtatttaaag gggcattcag cccctccgtc tatttgtgat tgcatccatt gatgtgtgta
     4861 ttattttgag cttgaaaaag ggaggcgctc tttaataata aataaatcta tcaaagctaa
     4921 atattaaatg gaagccaccc ttttttctct gatagtttta ttatcaccaa accatttatt
     4981 tcagctatcg aatctggatt cggccaggaa ggtaaactct tcacaattgg ataaatcttc
     5041 tctatttgtt ctttatttgc tggtttgcaa ttacagattc catagtaatt cataaatgca
     5101 accatcctaa aatagttgcc accatcccac caaaaaaaag attttccaaa agtgtctgcg
     5161 ctcatggaga gcatatttcc tgagtcgttt gaaccatata tatatatata ttttccgtta
     5221 agggaaatgc cttttgtttg agatgtgtat gatatttctt tagctaaaga gatatctttc
     5281 tgtcttgcga gagtatcatt caaaaataga tttttggatg ttgaaacgcc attaaatata
     5341 ataaataaaa aaaacatgca gtttaggtat tttatagagc gaaaattgct taagcaagaa
     5401 aacaaaatta ctgctactat aggcattaaa acaaacaggc gaggtggcgc ccctgagcca
     5461 acaacaacga taaatataac cggaagtata taaatgaaaa tgattgatga aatcaaataa
     5521 attgatcttg tttttaattt atatgttagg gtaaacatca gaattaataa aggaatcagt
     5581 aaccacttgt ataggtttaa accagtggga ggattgttat agaagttata tatattatct
     5641 atggctgatt taacaagcca tttaatatcg cttgcatttt gtatataatt tgagatgtac
     5701 tcgttcgatt caagcgaaaa ataatgcttg atagctttgg ttaatagcag gtaaattaag
     5761 gtagataaag ctatgaggga tagagataaa catgaactat aaaaagattc acgaatattt
     5821 ttctcatttc gtattatcga atttatctgc aacccaatga cgaatgcaat aatatatgtt
     5881 acgaatgttt ggtagattgc cattgaaaga atggacagta ctataccaga aaatatcaca
     5941 atcctatttt tttgcgagtg aaaaataatt gctgatatcg ctgccagtag aaaagcaatt
     6001 cccacagtat cagcttggtt aagaaactct aattgatagg agatctgagg gaaggtaata
     6061 aatactaaca tacctatcaa taattcataa gattctagct ttagcgatct gcagattata
     6121 aatgctgaaa tgatgataaa agataaggct attaatggtg ttatataaag tgaaaaaggc
     6181 tcagggagaa agtaatgtcg caaaaaagtg tgaaaccacc gcccaagcgt gattgtttga
     6241 taaaaattat ttgtaaactc gccatcaata ttaagtgttc catcgtacaa ccacccccaa
     6301 taacaataaa ttgatattgc aaaacatgat atgaaggcaa ggagatttgc atttttaaat
     6361 aaattaattt taatcatggg ttacctcgat gagatttttt tatgatgtat ttgggtctat
     6421 tttttacttc aatatagatt ctacctatat attctccgag aacaccaatc ccgatcagtt
     6481 gcactccacc caagaaaagt attgatacaa gcagggaggg atacccgcgt actgggttac
     6541 caaagacaag ggtgtctata atcatccatg caccatataa aaatgaaatg cttgcaacaa
     6601 acaagcctat ataagtccat acgcgaagag gaaaggttga aaaacttgtg ataccttcca
     6661 gtgccagatt ccataatttc cagccattaa attttgagat gccagcaaca cgctctgcgc
     6721 gtacatattc aacgacatcc gtcggaccac ccacccagct cagtatgcct ttcatgaaaa
     6781 gattgcgctc aggcaacagt ttaatgttct ccacaacctc acgagacatg agtcgaaaat
     6841 ctccgacatt ttcctcgatc tttggggtgc ttattttgtt gtgtaattta tagaaccact
     6901 cagctgtctt acgtttcagt cgtccatcag ttgagcggtc tgaacgttta gcaagcacca
     6961 tgtcagcacc tgcctgccac ttttcaataa gatgagggat aacctcaatt gggtcttgca
     7021 ggtcgacatc aataggaatt acagcatcgc cgcttgcatg gtctaaccct gcaaataagg
     7081 ctggttcttt accaaagttg cgtgtaaatg acagcggaac aactagcggg tctgaaacag
     7141 ccagcgcgtt aataattgac tctgtggcat ctttacttcc gtcatttatg aatacaattt
     7201 ctacttcata tggcttcaac tcttggaatt cacgtaccgt tttatagaaa acaggtatcg
     7261 cttcttcttc attgaagaca ggaacgacaa gagatatttt catttcgcat ccctaaagac
     7321 aatgaacttt gaatagacga aaccgcacac cagactgatg gcggagaagg tgataagagt
     7381 gactattgga ggaagtgagc atttatcagc tgcccaacca acaatcacac tcaatattcc
     7441 cataaatccc acgtataaca tgtagcgcat cgctgtagtc aatgctttga atgtgaatct
     7501 tgcattcgcg aagaagctaa agctcacagc cactacgaaa cctgtgaagt ttgccagagc
     7561 ctgactggta tgtgcggcat agatacatac accaaaaacc acccagtgta taagtgtgtt
     7621 cagcacacct atagatgtgt actttacaaa taactttaac atttatttaa tcaatgagct
     7681 ctgaaaggca tgaagtctat catccaagtc tcaattgatc gatacttgct atgtctgatg
     7741 agacaaaact gagacacata aggcctcaca atggcttgca aggctttaca tgttttgatg
     7801 tggtgggacg tgtgagcgca gtgttgatgg gataatcctt tgaattacaa gcggattctt
     7861 ataattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat ttaaatcaat
     7921 aagttacctc gcatttaagt aaactacgtt ctcctcttgt gccgtatttg tgccattgcg
     7981 acttataatc gcatcgattt tgctcgcgtg ctcggtaaga tgcccggctg aaaggtgggc
     8041 gtatctttga accatttcga gagtttccca tcctcccatc tctttaagcg caagaagaga
     8101 gacaccggac tgaaccagcc agcttgccca ggtatgcctc aggtcatgga agcggaagtt
     8161 gctaatgcct gcccgcttta acgctccctt ccatgccttg ttgctgtcgg ttctcatctt
     8221 ccttaccgct gctgtttttg ttccgtcgct tcggtaggca ggtttggtgt ggacaaacac
//
LOCUS       DADLEE010000148         8223 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000148 DADLEE010000000
VERSION     DADLEE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.151"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..304
                     /locus_tag="JQH51_003753"
     CDS             <1..304
                     /locus_tag="JQH51_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA packaging protein"
                     /protein_id="HAY6077933.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2186
                     /locus_tag="JQH51_003754"
     CDS             276..2186
                     /locus_tag="JQH51_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAY6077934.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2187..2390
                     /locus_tag="JQH51_003755"
     CDS             2187..2390
                     /locus_tag="JQH51_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077935.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2387..3980
                     /locus_tag="JQH51_003756"
                     /pseudo
     CDS             2387..3980
                     /locus_tag="JQH51_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3970..5475
                     /locus_tag="JQH51_003757"
     CDS             3970..5475
                     /locus_tag="JQH51_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAY6077936.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5512..5859
                     /locus_tag="JQH51_003758"
     CDS             5512..5859
                     /locus_tag="JQH51_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY6077937.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5917..6945
                     /locus_tag="JQH51_003759"
     CDS             5917..6945
                     /locus_tag="JQH51_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY6077938.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6997..7383
                     /locus_tag="JQH51_003760"
     CDS             6997..7383
                     /locus_tag="JQH51_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY6077939.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7395..7772
                     /locus_tag="JQH51_003761"
     CDS             7395..7772
                     /locus_tag="JQH51_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077940.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7759..>8223
                     /locus_tag="JQH51_003762"
     CDS             7759..>8223
                     /locus_tag="JQH51_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6077941.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1906 a         1932 c         2540 g         1845 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact
     2161 ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc
     2221 aggcgcgcct tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca
     2281 ggatcgaata tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt
     2341 cggttctggg tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc
     2401 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg
     2461 cgcaggattt ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc
     2521 cctgttgcct gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2581 tatcgcggct aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat
     2641 cagctaccgt ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt
     2701 cggggaggtg gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt
     2761 ggaagggaaa cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa
     2821 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     2881 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt
     2941 tctgcgggcc ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga
     3001 ggatgatttt ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac
     3061 cgggcgtccg gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc
     3121 taatcagttt tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacaca
     3181 gcttcagtcg gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac
     3241 cgaaaaggca tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat
     3301 taatattctg gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt
     3361 taaaattccg cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag
     3421 acaatggatt ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg
     3481 tttcctacga acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct
     3541 ccgccaatga gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg
     3601 ccacgcagat gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc
     3661 cacgggcgcg ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta
     3721 gctccggaag aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg
     3781 aggccggact gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca
     3841 ttttccgcca gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg
     3901 tgtggatagc ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga
     3961 caacatcacg tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg
     4021 cttctggagc ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca
     4081 gcaagccttt cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa
     4141 acggacgagt acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt
     4201 attgctgtac tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt
     4261 tccggaatga ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact
     4321 caggtacggg gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt
     4381 gactgcgctg acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat
     4441 gacacggcct gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc
     4501 cagacatccc gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat
     4561 ctggcacagg ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc
     4621 aatcagtttg aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg
     4681 gcgcgccgga tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt
     4741 atgggaacag aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat
     4801 gaattaatca atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat
     4861 acaggaggca ctatgccgca attaactgca acggaggctg ccgcgcagga gaaccagcga
     4921 gtgatgggga tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg
     4981 gcagggcaac agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg
     5041 caacaatctg gtgtatctgc gcagagtgaa gccgatcgca ttatggcatg tgaagaggcg
     5101 aaaggacgtg aacaactggc ggcaacgctg gcggcgatgc cggagatgac ggtggaaaaa
     5161 gcccgtccga ttctggctgc ttcaccgcag gcgaatgccg gaccctcact tcgtgatcag
     5221 attatggccc tggatgaggc aaaaggggca gaggcgcagg ctgaaaaact ggcggcctgt
     5281 ccggggatga ccgtggagaa tgcccgggct gtactggcgg cagcctcagg cagggcagaa
     5341 ccggtttctg catccacaac cgccatgttt gaacatttca tggcgaacca ttcaccggca
     5401 gcggtacagg gtggcgtgcc acagacgtca gcagacggtg atgcggacgt gaaaatgctc
     5461 atggccatgc catgaagtca gtgctgacca tcaatatgag gtttttacaa tatggtaacg
     5521 aaaaccatca ctgaacagcg tgcggaagta cgtatttttg ccggtaatga tccggcccat
     5581 accgccacag gctgcagcgg gatttcctcg gcaacaccgg ctctgacgcc cctgatgctg
     5641 gatgaggata ccgggaaact ggtggtctgg gacggacaga aggcaggcca tgtggttggc
     5701 atactggtac tgccgcttga aggtacagag acggtgctga cgtattacaa gtcggggacc
     5761 tttgcgacgg aggccatcca ctggcctgag agagtggata cacacaaaaa ggcgaatgcc
     5821 tttgccggca gtgccctgag tcacgcagcg ctgccgtaac attttatcag gtcactgcgg
     5881 tagcctgact gatttctgaa tgaaaggaac tgatttatgg gattgtttac gacccgccag
     5941 ttactcggtt ataccgaaca aaaagttaaa tttcgtgcgc tgtttctgga gctgtttttc
     6001 cgccgtacgg tgaatttcca taccgaagag gtaatgctgg acaaaattac cggaaaaacg
     6061 ccggtggcgg cctatgtctc cccggttgtt gaaggaaagg tgttgcgtca ccgtggcggt
     6121 gaaactcgtg tactgcgccc gggctacgtt aagccgaaac acgaatttaa ttaccagcag
     6181 gcggtggagc gtcttcccgg tgaagatccg gcccagctga acggcccggc ttaccgtcgc
     6241 ctgcgtatca tcaccgataa cctcaaacag gaagagcacg ccattgtcca ggtggaagag
     6301 atgcaggcgg tgaatgccgt gctgtatggc aaatacacca tggaaggaga gcagtttgaa
     6361 actgttgagg tcgattttgg ccggtcggtg ggaaataaca ttacgcaggc aggagggacg
     6421 gagtggagcg ctcaggaccg cgataccttt gatccgacgc atgatctgga tgcgtactgt
     6481 gattttgctt ccggcaccat caatattgcc attatggatg gtaaggtctg gcgtctgctg
     6541 aatggcttta agctgttccg cgaaaaactg gatacccgtc gtggctcaaa ttcacagctg
     6601 gaaacggcag tgaaagacct gggggcggtg gtgtccttca aagggtatta tggtgatctg
     6661 gcaatcgtgg tggcaaaaac gtcctatgtg gcagaggacg gtaccgaaaa acgttatctg
     6721 ccggacggca cactggtact gggaaatacg gccgcagagg gtattcgttg ctacggtgcc
     6781 attcaggatg cgcaggcatt gtctgaaggc gtggtggcgt cttcccgtta tccgaaacac
     6841 tggctgaccg tgggtgatcc ggcgcgtgaa ttcaccatga cgcagtccgc gccgctgatg
     6901 gtgctgccgg atccggatga gtttgtggtg gtgcaggtga aataatccgt gagcgggagc
     6961 taaatgctcc cgtttttcat tcaatcagga gcgggtatgg ctacgaaaga agaaaacctg
     7021 aaacgtcttt gtgagctggc tgagaggctg gggcgtgaac cggatgtctc cggtagtgcg
     7081 gcagatattg cgcagcgagt ggcggaactg gaggaagagc ttggagatgc gggggaggtc
     7141 gcagagccgg atacgtactc accatcgaaa gatgatccga ccgcgcataa aggcgagtca
     7201 gttcctgaac agccaggcat tgttaccgga gacgaaacgg ggcaggttac ggtggtggca
     7261 ctggcgacac tgcatacaaa atgtaatggt tcagtgatat ttgtttcacc gggaacgtcg
     7321 tttcgtgttt ctgctgaggt ggctgccagc atggcggcag gtggtctggc aaaaaggcag
     7381 taacaggaga tgtcgtggac ggtttcgata atctgtttga tgctgcgctt gccggtgtca
     7441 atgaagtcat tctccgtgat atggggatca gcgccgtcat tacgtccgga gaacttgagg
     7501 ggacacacct gacaggggtt tttgatgatc ccgaaagtat ttcttttgtt gccgggggga
     7561 tccggcttga ggattcctca ccgtgcctgt ttgtgaaaac ggcagatata tcgcagttac
     7621 gccgtcagga tacgttaact atcggggatg attctttctt tgtggatcgt attactccgg
     7681 atgacggggg atgctgttat atccggttaa ggcggggcag tcctgcccca cagaataatg
     7741 ccagaatgag gcgatatgat gaagggactt gagaatgcca tccgcaatct gaacagcctc
     7801 gatacccgca tggtgccaca gaccagcgca tgggcggtaa accgtgtggc ggcaaagatt
     7861 gtttctgtcg ccacacggca ggttgcgcag aataccgttg ccggggataa ccaggtgaaa
     7921 ggtattcccc tgaaaacggt cagggagcgt gtcaggctgc ttaaagccag cccctcagga
     7981 aaaatgtatg cccgcatgcg tgttaaccgg ggtaacctgc ccgccattaa gctgggtacc
     8041 gcacaggtca gactggcccg ctcccggcat ggcagcaact cacgtcatcg tggcagcgta
     8101 ctgaaagtgg ggaaatacct tttccgggat gcgtttattc agcaactggc gaatggtcgc
     8161 tggcatgtga tgcggcgtat tgacggcaaa aatcgttacc ccattgatgt ggtgaaaatc
     8221 ccg
//
LOCUS       DADLEE010000149         8070 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000149 DADLEE010000000
VERSION     DADLEE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.152"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            292..619
                     /locus_tag="JQH51_003763"
                     /pseudo
     CDS             292..619
                     /locus_tag="JQH51_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="JQH51_003764"
     CDS             complement(649..3561)
                     /locus_tag="JQH51_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077942.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3564..3680
                     /locus_tag="JQH51_003765"
     CDS             3564..3680
                     /locus_tag="JQH51_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY6077943.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4044..4886
                     /locus_tag="JQH51_003766"
     CDS             4044..4886
                     /locus_tag="JQH51_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY6077944.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="JQH51_003767"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="JQH51_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY6077945.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="JQH51_003768"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="JQH51_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY6077946.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..7941
                     /gene="lpxM"
                     /locus_tag="JQH51_003769"
                     /gene_synonym="msbB"
     CDS             6997..7941
                     /gene="lpxM"
                     /locus_tag="JQH51_003769"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY6077947.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2170 a         1810 c         1925 g         2165 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 acccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7321 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaattcg gcacaataac
     7381 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7441 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7501 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7561 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7621 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7681 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7741 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7801 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7861 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7921 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7981 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     8041 gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DADLEE010000150         7910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000150 DADLEE010000000
VERSION     DADLEE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.153"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(123..953)
                     /locus_tag="JQH51_003770"
     CDS             complement(123..953)
                     /locus_tag="JQH51_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY6077948.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="JQH51_003771"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="JQH51_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY6077949.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="JQH51_003772"
     CDS             2379..2729
                     /locus_tag="JQH51_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY6077950.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="JQH51_003773"
     CDS             2855..3415
                     /locus_tag="JQH51_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6077951.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="JQH51_003774"
     CDS             3418..6384
                     /locus_tag="JQH51_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY6077952.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="JQH51_003775"
     CDS             6451..6828
                     /locus_tag="JQH51_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6077953.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="JQH51_003776"
     CDS             complement(7029..7688)
                     /locus_tag="JQH51_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY6077954.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2242 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DADLEE010000151         7880 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000151 DADLEE010000000
VERSION     DADLEE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.154"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..1330
                     /gene="yjeM"
                     /locus_tag="JQH51_003777"
                     /pseudo
     CDS             1..1330
                     /gene="yjeM"
                     /locus_tag="JQH51_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313164.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1481..4792)
                     /gene="mscM"
                     /locus_tag="JQH51_003778"
     CDS             complement(1481..4792)
                     /gene="mscM"
                     /locus_tag="JQH51_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY6077955.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4814..5782)
                     /gene="psd"
                     /locus_tag="JQH51_003779"
     CDS             complement(4814..5782)
                     /gene="psd"
                     /locus_tag="JQH51_003779"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY6077956.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5879..6931)
                     /gene="rsgA"
                     /locus_tag="JQH51_003780"
     CDS             complement(5879..6931)
                     /gene="rsgA"
                     /locus_tag="JQH51_003780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY6077957.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7026..7571
                     /gene="orn"
                     /locus_tag="JQH51_003781"
     CDS             7026..7571
                     /gene="orn"
                     /locus_tag="JQH51_003781"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY6077958.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7782..7857
                     /locus_tag="JQH51_003782"
     tRNA            7782..7857
                     /locus_tag="JQH51_003782"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7815..7817,aa:Gly,seq:gcc)
BASE COUNT         1785 a         2041 c         2134 g         1920 t
ORIGIN      
        1 taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggtggtatc tattcctgga
       61 tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg ttttcctctt
      121 atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca acattcctct
      181 atggtagtga catgacccag cactggcgta ttgccggact ggagcctacg caggtggttg
      241 gtctgctggc agtagcatgg atgattctgg tcacagtcgt tgcttcaaag gggattaata
      301 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
      361 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
      421 tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg tttgtggtat
      481 ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
      541 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
      601 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
      661 gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg gtgatgctgg
      721 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
      781 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt tgctattcac cgctgaaagc
      841 catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc tgaatgcgat
      901 ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct tcattctgct
      961 ggtttcgttt ggtggcggta ccgcatcggc gttctttaac aagctgacgc tgatggcgaa
     1021 cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccgtttt ttaaagcacg
     1081 tcaggatctc gacagaccat ttgtgatttt caaaacgcgt atgtcggcaa tgattgcgac
     1141 ggtggttgtc gttctggtgg tgacatttgc gaacgtcttc accattattc agcctgtggt
     1201 tgaagccgga gactgggaca gcacattgtg gatgattggc ggccctgtct tcttctcgct
     1261 gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaataaac ctgagttagc
     1321 tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga gttgtcagga
     1381 agtaatgcgt atggatgata ctctccaggg ccggtgataa ctgaagatga tgttaagctg
     1441 ctaaatgagc cacgttgaca tcatttggca gcttcatgca ttacaaactt cccgcctgac
     1501 gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac
     1561 gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca
     1621 gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca
     1681 gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt
     1741 cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcactgt
     1801 tggcatcggc aggggccgga atcgtcaaca ctacgcgcgt gaccgagtca gagagcgacc
     1861 agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc
     1921 agtcgctgat ggtggtggcg cgggtgttaa tttttgtcac gctaccagta agatcgcgga
     1981 tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag gatgatcagg ccagagataa
     2041 agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc
     2101 actgcaattt cgaccactca ataccaatca ttgagaagcc gaccagcccg ccaatcagca
     2161 tcagcagata ttttgtgatg gtggtgatgg cgtaacccgt acccggcgtt aaatccaggt
     2221 gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga
     2281 taaataccag aatggcaatc agcaccgcac cgagggtaat tggctccaga ctttctacgc
     2341 cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat
     2401 ggatttctga ccacagcaca atgaccgaaa gcagggcgat caacatcaaa attgagcgca
     2461 ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa
     2521 ttgctccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgca cgttgcgcta
     2581 acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca
     2641 tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt
     2701 caagcctcgc taacagtgcc tgcgccgttg ccagataacc caccgccgac gccagaatgg
     2761 caaccagtgg tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc
     2821 tgccttcttt gttgagatac aacggaatac cggctttttt caggcttagg gtgaccaccg
     2881 ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggaa aattcgcggt
     2941 cgtcgaggtt atcgaacatc atcagcgcca taatcagccc gatgctcatc aggtagtagc
     3001 gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg
     3061 ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag cggcacggtg gccgttacac
     3121 catcgccaat cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg
     3181 tcatccacag caccggtaac ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg
     3241 tcagccagaa gtgatcctga gtcactttgc cgactttcgc cgccgaacgt tcaagaaaac
     3301 gggtgaaata gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg
     3361 gcaaaattgt ctctttgctg gtcagcatca tcacactggc tttgcccaac tgactgaagg
     3421 tgtccagcga aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca
     3481 tcgggcgcac gtcagaggcc cagaacagat agcggtgcgt tgcttcgttc acctctttca
     3541 gcgcatcctc cagttgcctg ttggagactt tcagcttggt cagttccaat agtagcgtgt
     3601 cgccaccctg caacaatgag ttcagcaact cacgctgagt gcgcagttgt gcttccagaa
     3661 tacggttttg ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagcagcg
     3721 gctgtttatt aagtaaatcc tcataacgta accgttgcac acgcaactgc gccatttcgg
     3781 tatcaagctg ttgtggtttt ggcatttctg gtagccgtgc cacctgcgcc cgcagcgctt
     3841 cgccgagcag gttggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgcct
     3901 gccggacctg taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agctccatcc
     3961 gctgcgcctg ttgattcagc gctgccgata gttcgcggtt aattttgaat tgcgcgacga
     4021 tatctttcgg caaatcggca ctgttttctg ccagtaattc ggtactttcc agcgcccgct
     4081 ccgcctcaag ctgacgttgg ctattcaact gattacgtaa ggcctgcaaa tacgcatcca
     4141 gttgctggct ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat
     4201 tggcagacag ctgcgccagc tccagttcat caacgagcgc cttaagacgt gcagagtcag
     4261 actgcaacgc gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc
     4321 ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc agttgattca
     4381 gcgaatcggc aatctcgcgg gcgcgctcct gctcttgctg ggcctgacgg cttttatcca
     4441 gcaactggct gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggcg
     4501 acacgctgcg cggctcgtca cgaatgttgt ttaattgtgc gcgcagagta gcggagagtt
     4561 tcggataatt atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt
     4621 cctcaagcgc atttaaggca gactggagcg cctctacgac ttccggctgt gcgggtttcg
     4681 ccgcttttgc ctgctccagt tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt
     4741 acgcccccca actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt
     4801 ttcctttgat ggattagacc tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt
     4861 ctgcttcgat ctcttcagca ggaagtgggg cgggttcagc gtctggcgta acaaaggttt
     4921 cggtagatac tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa
     4981 ccagattcac tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac
     5041 ccatttcctg gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc
     5101 agcgcttgat gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc
     5161 tgccaacaat cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa
     5221 ggcaaatcac gcgttcgtta cgggcaaaca gatttggcac gttctgagcg gtgagatggt
     5281 taacggaaaa gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca
     5341 tgtgtacgcg atggtagtca cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga
     5401 acaggtccgc catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt
     5461 tggcttgcag gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag
     5521 gcatgaccag tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga
     5581 caaagaattc gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt
     5641 cgaccttgta gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct
     5701 tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg
     5761 aaagtttaaa tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg
     5821 gccaacatgc cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc
     5881 agtcatccgt atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac
     5941 ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct tcccggatag
     6001 cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat
     6061 ggaattcgac aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac
     6121 gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg
     6181 tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc gttggtcagg atctcttttt
     6241 gcagccccag caacgcgttc agcaggctgg atttaccgac gccagactgc ccggcaaaaa
     6301 tgctgatgcg cccggtcaac gcctcttcca gcggtttcag cccatcctga gtatggctgg
     6361 aaaccatcaa tacgcgataa ccgatgttgc ggtagatatc catctgctcg ttgacgaacg
     6421 ccatgccttc gtcgtccagc aggtctatct tgttgagcac gataatcggc tcaatctgca
     6481 aggtttcgca ggccaccagg taacggtcga taatattgag cgacagctcc ggcaaaatgg
     6541 cggagacaat gacaatctgg tcgatgttgg cggctatggg tttcacgccg tcgtagaaat
     6601 ccggacgcgt taacaccgag gtgcgctcat gcaccgcttc cacgatccct ttgacattta
     6661 cgccttccgc cgccggttta cccggacgcc agactacgcg gtcgccggtt accagcgaac
     6721 ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt
     6781 gcataccaaa gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt
     6841 agtcgggctt ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct
     6901 gctggccttt ggagagttta tttttactca atcgtactga ctcctggtcg cccctgatgg
     6961 gcaaaacatc tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa
     7021 atagcatgag tgccaatgaa aacaacctga tttggatcga tcttgagatg accggtctgg
     7081 atcccgagcg cgatcgcatt attgagattg ccacgctggt gaccgatgcc aatctgaata
     7141 ttctggcaga agggccgacc attgcagtac atcagtccga tgaacagctg gcgctgatgg
     7201 atgactggaa cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg aaagcgagca
     7261 cgatgggcga tcgggaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg
     7321 cgggaaaatc gccgatttgc ggtaacagca tcggacagga ccgtcgtttc ctgtttaaat
     7381 acatgccgga gctggaagtc tacttccact accgttatct cgatgtcagc accctgaaag
     7441 agctggcgcg ccgctggaag ccggaaattc tggatggttt taccaagcag gggacgcatc
     7501 aggcgatgga tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta
     7561 tcaagctgta aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat
     7621 tttgacgcta aatcgaacgt cttgctgata atttcagcac ttgagataaa aacgcaaaaa
     7681 aaactttttt ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga
     7741 cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta cgcgggaata gctcagttgg
     7801 tagagcacga ccttgccaag gtcggggtcg cgagttcgag tctcgtttcc cgctccaaaa
     7861 tttaaaagtg ctgtaaggca
//
LOCUS       DADLEE010000152         7746 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000152 DADLEE010000000
VERSION     DADLEE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.155"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1015
                     /locus_tag="JQH51_003783"
     CDS             <1..1015
                     /locus_tag="JQH51_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6077959.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="JQH51_003784"
     CDS             1104..2564
                     /locus_tag="JQH51_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY6077960.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="JQH51_003785"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="JQH51_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY6077961.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /locus_tag="JQH51_003786"
     CDS             complement(2981..3667)
                     /locus_tag="JQH51_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6077962.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="JQH51_003787"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="JQH51_003787"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY6077963.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="JQH51_003788"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="JQH51_003788"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY6077964.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="JQH51_003789"
     CDS             complement(6729..7247)
                     /locus_tag="JQH51_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY6077965.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7746
                     /gene="ydeI"
                     /locus_tag="JQH51_003790"
     CDS             7523..>7746
                     /gene="ydeI"
                     /locus_tag="JQH51_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY6077966.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1812 c         1957 g         2036 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcttg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagg
//
LOCUS       DADLEE010000153         7721 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000153 DADLEE010000000
VERSION     DADLEE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.156"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..832)
                     /locus_tag="JQH51_003791"
     CDS             complement(<1..832)
                     /locus_tag="JQH51_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HAY6077967.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="JQH51_003792"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="JQH51_003792"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY6077968.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /locus_tag="JQH51_003793"
     CDS             complement(2481..2708)
                     /locus_tag="JQH51_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY6077969.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="JQH51_003794"
     CDS             complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="JQH51_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY6077970.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="JQH51_003795"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="JQH51_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY6077971.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="JQH51_003796"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="JQH51_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY6077972.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="JQH51_003797"
     CDS             complement(5530..6513)
                     /locus_tag="JQH51_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6077973.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="JQH51_003798"
     CDS             complement(6559..7383)
                     /locus_tag="JQH51_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY6077974.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="JQH51_003799"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="JQH51_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY6077975.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DADLEE010000154         7538 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000154 DADLEE010000000
VERSION     DADLEE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.157"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..1157
                     /gene="rbsA"
                     /locus_tag="JQH51_003800"
                     /pseudo
     CDS             1..1157
                     /gene="rbsA"
                     /locus_tag="JQH51_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1162..2127
                     /gene="rbsC"
                     /locus_tag="JQH51_003801"
     CDS             1162..2127
                     /gene="rbsC"
                     /locus_tag="JQH51_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY6077976.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2152..3042
                     /gene="rbsB"
                     /locus_tag="JQH51_003802"
                     /pseudo
     CDS             2152..3042
                     /gene="rbsB"
                     /locus_tag="JQH51_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3168..4097
                     /gene="rbsK"
                     /locus_tag="JQH51_003803"
     CDS             3168..4097
                     /gene="rbsK"
                     /locus_tag="JQH51_003803"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY6077977.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4101..5093
                     /gene="rbsR"
                     /locus_tag="JQH51_003804"
     CDS             4101..5093
                     /gene="rbsR"
                     /locus_tag="JQH51_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY6077978.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5059..6486)
                     /locus_tag="JQH51_003805"
     CDS             complement(5059..6486)
                     /locus_tag="JQH51_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY6077979.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6509..7201)
                     /locus_tag="JQH51_003806"
     CDS             complement(6509..7201)
                     /locus_tag="JQH51_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY6077980.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1915 a         1830 c         1986 g         1807 t
ORIGIN      
        1 accatgtatg tcgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac
       61 aagttagtgg gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg
      121 agctttgagt cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa
      181 accgaatccc tgttccgcgt catccgcgag ctgaaatcgc aaggccactg tattgtctat
      241 atctcccacc gcatgaaaga aatcttcgag atttgcgatg acgttaccgt ttttcgtgat
      301 gggcaattta ttgctgagcg cgaagtggca tcactgaccg aagattcgct gattgagatg
      361 atggtgggcc gcaagctgga agatcaatat ccacacctgg acaaagcgcc aggagatatc
      421 cgcctgaaag tcgataatct ctgcggacct ggcgttaacg atgtctcttt tactttacgc
      481 aaaggcgaaa ttcttggcgt ctctggtttg atgggcgcag gtcgtaccga actgatgaaa
      541 gtgctctacg gcgcactgcc gcgcaccagc ggttacgtca ccctggatgg gcatgaagtc
      601 gttacccgtt caccgcagga tggcctggca aacggcattg tgtatatctc cgaagaccgt
      661 aaacgtgacg gtttagtgct gggcatgtca gtaaaagaga acatgtcgct gacagcgctg
      721 cgctacttca gccgcgctgg cggcagtttg aagcatgccg atgaacagca ggctgtgagt
      781 gatttcattc gtctgtttaa tgtgaaaacg ccgtcgatgg aacaggcaat tggtctgctt
      841 tccggtggca atcagcaaaa agtggcgatt gcccgcggtc tgatgacacc cccaaagtat
      901 tgatccttga tgagcctacc cgtggcgtag atgtcggcgc gaaaaaagag atctatcaac
      961 tgattaacca gttcaaagcc gatggcttga gtatcattct ggtgtcatcg gagatgccag
     1021 aagtattagg catgagcgat cgcatcatcg tcatgcatga agggcatctc agcggggaat
     1081 ttactcgtga gcaggccacc caggaagtgt taatggctgt cgctgtgggc aagcttaatc
     1141 gcgtgaatca ggagtaaaaa aatgataacc cagactgtct ctggtcgccg ttatttcacg
     1201 aaagcgtggc tgatggagca gaaatcgctt atcgctctgc tggtgctgat cgcgattgtc
     1261 tcgacgttaa gcccgaactt tttcaccatc aataacttat tcaatattct ccagcaaact
     1321 tcggtgaacg ccattatggc ggtcgggatg acgctggtga tcctgacgtc gggcatagac
     1381 ttgtcggtag gttctctgtt ggcgctaacc ggcgcagttg ctgcatctat cgtcggcatt
     1441 gaagtcaatg cgctggtggc tgtcgctgct gctctcacgt taggtgccgc aattggtgcg
     1501 gtaaccgggg tgattgtagc gaaaggtcgc gtccaggcgt ttatcgctac gctggttatg
     1561 atgcttttac tgcgcggcgt gaccatggtt tataccaacg gtagcccagt gaataccggc
     1621 tttactgaga acgccgatct gtttggctgg tttggtattg gtcgtccgct gggcgtaccg
     1681 acgccagtct ggatcatggg gattgtcttc ctcgcggcct ggtacatgct gcatcacacg
     1741 cgtctggggc gttacatcta cgcgctgggc ggcaacgaag cggcaacgcg tctttctggt
     1801 atcaacgtca ataaaatcaa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg
     1861 gccgggatca ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat
     1921 gagctggatg ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt
     1981 cgcattgttg ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat
     2041 ttgttaggtg tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg
     2101 gtgctggtag acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg
     2161 aaaaaactgg ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg
     2221 atggcaaaag acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg
     2281 ctgaaagatg gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac
     2341 tcccagaaca acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc
     2401 aaaattctgc tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct
     2461 aactaggcga acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg
     2521 agccacattg catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag
     2581 aaagcgggtg aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc
     2641 cgtgaacgtg gcgaaggctt ccagctggcc gttgctgctc acaagtttaa tgttcttgcc
     2701 agccagccag cagattttga tcgcaccaaa ggtttgaacg taatgcagaa cctgttgacc
     2761 gctcatccgg atgttcaggc tgtattcgcg cagaatgatg aaatggcgct gggcgcgctg
     2821 cgcgcactgc aaactgccgg taaatcggat gtgatggtcg tcggatttga cggtacaccg
     2881 gatggcgaaa aagcggtgaa tgatggcaaa ctagcagcga ctatcgctca gctacccgat
     2941 cagattggcg cgaaaggcgt tgaaaccgca gataaagtgc tgaaaggcga gaaagttcag
     3001 gctaagtatc cggttgatct gaaactggtt gttaagcagt agttttaatc aggttgtatg
     3061 acctgatggt gacataaata cgtcatcgac agatgaacgt gtaatataaa gaaaagcagg
     3121 gcacgcgcca ccctaacacg gtggcgcatt ttatggacat cccgaatatg caaaacgcag
     3181 gcagcctcgt tgttcttggc agcattaatg ctgaccacat tcttaatctt caatcttttc
     3241 ctactccagg cgaaaccgta accggtaacc actatcaggt tgcatttggc ggcaaaggcg
     3301 cgaatcaggc tgtggctgct gggcgtagcg gtgcgaatat cgcgtttatt gcctgtactg
     3361 gcgatgacag cattggcgag agcgttcgcc agcagctcgc cactgataac atcgatatta
     3421 ctccggtcag cgtgatcaaa ggcgaatcaa caggtgtggc gctgattttt gttaatggcg
     3481 aaggtgagaa tgtcatcggt attcatgccg gcgctaatgc tgctctttcc ccatcgctgg
     3541 tggaagcgca acgtgagcgt attgccaacg cgtcagcgtt attaatgcag ctggaatcac
     3601 cactcgaaag tgtgatggca gcggcgaaaa tcgcccatca aaataagact atcgttgcgc
     3661 ttaacccggc tccggctcgc gaacttcctg acgaactgct ggcgctggtg gacattatta
     3721 cgccaaacga aacggaagca gaaaagctca ccggtattcg tgttgaaaat gatgaagatg
     3781 cagcgaaggc ggcgcaggta ctgcatgaaa agggtatccg tactgtactg attactttag
     3841 gaagtcgtgg tgtatgggct agcgtgaatg gtgaaggtca gcgcgttcct ggattccggg
     3901 tgcaggctgt cgataccatt gctgccggag atacctttaa cggcgcgtta atcacggcgt
     3961 tgctggaaga aaaaccattg ccagaggcga ttcggtttgc ccatgctgcc gctgcgattg
     4021 ccgtaacacg taaaggcgca caaccttccg taccgtggcg tgaagagatc gacgcatttt
     4081 tagacaggca gaggtgacgc ttggctacaa tgaaagatgt tgcccgtttg gcgggcgttt
     4141 ctacctcaac agtttctcac gttatcaata aagatcgctt cgtcagtgaa gcgattaccg
     4201 ccaaagttga agcggcgatt aaagaactca attacgcgcc atcagctctg gcgcgtagcc
     4261 tcaaactcaa tcaaacacat accattggca tgttgatcac tgccagtacc aatcctttct
     4321 attcagaact ggtgcgtggc gttgaacgca gctgcttcga acgcggttat agtctcgtcc
     4381 tttgcaatac cgaaggcgat gaacagcgga tgaatcgcaa tctggaaacg ctgatgcaaa
     4441 aacgcgttga tggcttactg ttactgtgta ccgaaacgca tcagccttcg cgtgaaatca
     4501 tgcaacgtta tccgacagtg cctaccgtga tgatggactg ggctccgttc gatggcgaca
     4561 gcgatcttat tcaggataac tcgttgctgg gcggagactt agcaacgcaa tatctgatcg
     4621 ataaaggtca tacccgtatc gcctgtatta ccggtccgct ggataaaact ccggcgcgcc
     4681 tgcggttgga aggttatcgg gcggcgatga aacgtgcggg tctcaacatt cctgatggct
     4741 atgaagtcac tggtgatttt gaatttaacg gcgggtttga cgccatgcgc caactgttat
     4801 cacatccgct gcgtcctcag gccgtcttta ccggaaatga cgctatggct gttggcgttt
     4861 accaggcgtt atatcaggca gagttacagg ttccgcagga tatcgcggtg attggctatg
     4921 acgatatcga actggcaagc tttatgacgc caccattaac cactatccac caaccgaaag
     4981 atgaactggg ggagctggcg attgatgtac tcatccatcg gataacccag ccgacccttc
     5041 agcaacaacg attacaactt actccgattc tgatggaacg cggttcggca tagatttacg
     5101 ctgtcttttg atcaaattat taccatcggt tgttttcaga agcatgaaca ttgctgctga
     5161 agcaacagta ataatgccca tcgtgataaa cgtatagtgg aattgttcga cagtcgttgt
     5221 gccttccatc ccttcataaa tgcgaaggac ggccgcactt acagcaacgc ctaaactaat
     5281 cgacagttgc tgcgtgaccg ccagaacact gttaccgctg ctggcgttgt catcggtcag
     5341 atcggcaagt gtgatggtat tcatcgcggt aaactgcgtc gacatagcca tccctaatat
     5401 aaacaacggc aagatcagca tccatatcgc cattgccggt gattgcaaag agaactgagc
     5461 gatcattagc ccaataatca ccgtgatccc cactaatgta tggcgatagc ccagacgacg
     5521 taagacttgg gtaaccatcg attttgcaat aatggaacct aacgctgtcg gtgccatcat
     5581 acagccggca ataaacgcct gataaccaaa tcctacctgt aacatcaatg gcataaggaa
     5641 cggtacacag ccggtcccca gacgggttgc aatattgcct acgataccga tcgagaaagt
     5701 gcgggtttta aataaatcta atgaaattaa tgggtttggc gtacgtcgcg catggagaat
     5761 atagagaagc agtaacccga tgctggtgac aattaccgtc aaggcaatcc agctggcgac
     5821 aatcttttcc ccgaatagct ctattccgct tgaaaagaga acaaggctga ggccaaacag
     5881 caaaaagcca gtgatatcga atctgcgtcg tgcggtggtg aaattgggca tatgtttgcg
     5941 cgcgtaaaga aggcccgcaa tacctatggg gatattaatt aaaaatatcc agtgccaggt
     6001 tgcccaggtg accagcacgc cgccaagaac ggggcctaaa attggcccca ccagacccgg
     6061 catggcgaca aaattcaata ccggaagaag ttcattacga ggataagcac gcagtaaggc
     6121 cggccgagca acaggcatca tcattgcgtc gcctatccct tgaataaccc ggaagacaac
     6181 cagctgtggt agcgaattag aaagtgcgca ggccagagaa cccaacgtga acagactcac
     6241 ggcaagggta aaaatgcgac gcgtaccgaa gcgatcggct agccatccgc ttaccggaat
     6301 aagcatcgcc accgtcagcg tataactgat gatggctgat tgcatcgcga gaggagaacg
     6361 attaaggcta tgagcgattg cgggtaaggc ggtattcaga atagtggcat caagtgcctg
     6421 catgaagaag gccatcgccg cgatccacgg caaacccgac atactacgct tctttttatc
     6481 gctcattcaa tgtcctgtta tcgggttatc acttatcagg tgagcgtagc agcgcctgac
     6541 aagctttaaa tgccgcgtcg ccatcgcttt ggataatcgc atcgacaatc gcctggtgca
     6601 gatccagctt tatcactgtg tcgctggtaa ttgacgtgaa gtaagtgtga taaaccgaat
     6661 ggaatagcga ggcgaatgag gtcaaaaacg gattggcgct catttcatag atatgctcat
     6721 gccaggccat atcgacttcg atccagcgtt cacggcgaaa gttctctttt aatgccgcca
     6781 tttcggccat taacgtattg agatgcgcct tctgttccgc ggtgccaacc gttgctgcca
     6841 gtaggcaggc ttgcggctcc agacagatac gcataaccag aaagtgatcg atgacctgat
     6901 gaaagttctc ttctgttatc caccaagtaa gcaattcctg atcaagaaaa ttccagttcg
     6961 attgtggcat gacccgagta ccaattcgcg gtcgcggtaa aaccatccct tttgccgtta
     7021 acgttttgac cgcttcgcgt accgctgtac gactcactcc aaattgctcg cccagctcaa
     7081 tctcaccggg caaaatggtg ccgggttcat attcaccttt taagatccgt tgcgccagct
     7141 tctcagccag aacatacgaa aggtttttct gtgcagctaa ctgttgtgcg cttaaaggca
     7201 ttacttatct tcctttttct ttttattcct ccttagtatg ccaccaggaa gtgtgattac
     7261 ggttgcaaaa acggcaaatt gcttgtttta tggcacatta acggggtgtt ttgctgaaaa
     7321 aatgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccgggata actccctata
     7381 atacgccacc actgacacgg aacaacggca aatacgccgc cgggtcagcg gggttctcct
     7441 gagaattccg acagaaaaag caaaaataaa tgattgactc tgtagcggga aagcgtatta
     7501 tgcacacccc ccccccgcgc cgctgagaaa aagcgaag
//
LOCUS       DADLEE010000155         7374 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000155 DADLEE010000000
VERSION     DADLEE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.158"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            51..1541
                     /locus_tag="JQH51_003807"
     CDS             51..1541
                     /locus_tag="JQH51_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY6077981.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1593..2750
                     /locus_tag="JQH51_003808"
     CDS             1593..2750
                     /locus_tag="JQH51_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY6077982.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="JQH51_003809"
     CDS             complement(2716..2838)
                     /locus_tag="JQH51_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077983.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3041..4726
                     /locus_tag="JQH51_003810"
                     /pseudo
     CDS             3041..4726
                     /locus_tag="JQH51_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4764..7124
                     /locus_tag="JQH51_003811"
     CDS             4764..7124
                     /locus_tag="JQH51_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY6077984.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7169..>7374
                     /locus_tag="JQH51_003812"
     CDS             7169..>7374
                     /locus_tag="JQH51_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6077985.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDL
                     RYMIYPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2163 a         1615 c         1567 g         2029 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     2941 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3001 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3061 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3121 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3181 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3241 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3301 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3361 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3421 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3481 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3541 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3601 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3661 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3721 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3781 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3841 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     3901 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     3961 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4021 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4081 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4141 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4201 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4261 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4321 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4381 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4441 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4501 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4561 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4621 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4681 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4741 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4801 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4861 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     4921 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     4981 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5041 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5101 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5161 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5221 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5281 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5341 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5401 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5461 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5521 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5581 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5641 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5701 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5761 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5821 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     5881 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     5941 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6001 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6061 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6121 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6181 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6241 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6301 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6361 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6421 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6481 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6541 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6601 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6661 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6721 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6781 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6841 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     6901 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     6961 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7021 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7081 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7141 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7201 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7261 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7321 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt catt
//
LOCUS       DADLEE010000156         7187 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000156 DADLEE010000000
VERSION     DADLEE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.159"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="JQH51_003813"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="JQH51_003813"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY6077986.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /locus_tag="JQH51_003814"
     CDS             complement(986..2344)
                     /locus_tag="JQH51_003814"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY6077987.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JQH51_003815"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JQH51_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY6077988.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="JQH51_003816"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="JQH51_003816"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY6077989.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="JQH51_003817"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="JQH51_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5310
                     /gene="msrQ"
                     /locus_tag="JQH51_003818"
     CDS             4675..5310
                     /gene="msrQ"
                     /locus_tag="JQH51_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY6077990.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5568..6218
                     /gene="zinT"
                     /locus_tag="JQH51_003819"
     CDS             5568..6218
                     /gene="zinT"
                     /locus_tag="JQH51_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY6077991.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2031 a         1630 c         1507 g         2019 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgaccg
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga tattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgatctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctagcaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcct tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa gcaattttta aaagaatcag atgttacggc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaagtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgccgggctg
     5221 gctgtactgc ttttagcctt acggtataag aagttgcttt cccttttcaa ccggttacgc
     5281 aagcaggccc acaacaaatt atctctataa cttcatattg ccgacaaact tcgcgttatc
     5341 ccggccggat gcggcgcgag cgccctatcc ggcccacaca gatcgcatca agttatgttt
     5401 cgtttgtcat caacctttca tcaccagaat aatttatttt atttcaataa gatagttcaa
     5461 tacacctgag aataccatgc tctcatttcc taatgagttg ttgcattttt tcatatgtta
     5521 caatataaca ttacacataa tgtacattaa ctctggagga aattgttttg gcgattcgtc
     5581 ttcataaact ggctgttgct ttaggtgtct ttattgttag cgctcctgcc ttttcgcatg
     5641 gtcatcattc tcatggcaaa cccttaacag aggtcgaaca aaaagctgct aatggtgttt
     5701 ttgatgatac caatgtacaa aaccgaacgc tcagtgactg ggatggagtc tggcaatccg
     5761 tttacccttt actgcaaagt ggcaaacttg atcccgtctt tcagaagaaa gcggatgcag
     5821 ataaaaccaa aacatttgct gaaattaaag attattatcg caaaggttat ccgacagata
     5881 tcgaaatgat tggcattgag gacggcattg tcgaattcca tagaaataat gaaacaacat
     5941 cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc aagaaaggcg
     6001 ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat atacaattta
     6061 gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg ggtaatgatt
     6121 cccagcaatc tttattgaat gaaatggaaa actggccaac gtattatccc tatcagttga
     6181 gtagcgaaga agtggtcgag gagatgatgt ctcattgatt tttttgcgct gacttcagtt
     6241 atttaacaga acagtgcctg gtagtaacaa caataattta ccaagacaaa taagtaagac
     6301 aaactcagac acgctcatac ttgagtcgtg tcttttttat tatgtctgta gaaaaaaact
     6361 gagaaaaagg ctaacaataa atacacaact cagccataca gactggcagc gcaaatgttt
     6421 ccaggagcaa gtcatctgtc atggtaaagc taaccagaaa tataggttaa ttttggtgtt
     6481 taaacctttt ttacttgtta gtatgataca aaggctttca aaaaagctgg gaaattcaac
     6541 aactcttgga aattatggac tttgtcccgc ggctttaatc tgacggccgc gttccttttt
     6601 tggctcaatt attgacccct tctctacgca acttcagttc ccgccagcaa ctttgcggca
     6661 gtttcatagg atcaatgtct aaaagaataa tggtgaccga taagaaaacg actgaataac
     6721 tgtagatttt cgctcgcaac cttcctgtca gctccatagc gaatcaagcg ctgaatgtca
     6781 cagtatcgaa cagaaaacag tgacgatcta acccttcaag aatattctac gattgttctg
     6841 tttaggaaaa gcaaggcggg aagtcgggag ataagtcatt gataaaatgg cggagagagg
     6901 gggatttgaa cccccggtag agttgcccct actccggttt tcgagaccga tgatacagag
     6961 tcgaaaaatc aaagagttaa aaatttttct tggaaaaaat gtcttaaaat cgtacacgaa
     7021 aaatcaatga attacataac attaaagaag aatattccaa gccttcttga gtccaccacc
     7081 tgttttcagg tgtcattcag acgtgatttg ccccagattg ggaaagcacg cttttttctc
     7141 agcttattcc gttttgcctg tgtgttgaat aattccactg cagttat
//
LOCUS       DADLEE010000157         7185 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000157 DADLEE010000000
VERSION     DADLEE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.160"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="JQH51_003820"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="JQH51_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="JQH51_003821"
                     /pseudo
     CDS             761..913
                     /locus_tag="JQH51_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="JQH51_003822"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="JQH51_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY6077992.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="JQH51_003823"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="JQH51_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4178..4375
                     /locus_tag="JQH51_003824"
                     /pseudo
     CDS             4178..4375
                     /locus_tag="JQH51_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4419..4538
                     /locus_tag="JQH51_003825"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="JQH51_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="JQH51_003826"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="JQH51_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY6077993.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /locus_tag="JQH51_003827"
     CDS             5983..6723
                     /locus_tag="JQH51_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY6077994.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7083..>7185)
                     /locus_tag="JQH51_003828"
     CDS             complement(7083..>7185)
                     /locus_tag="JQH51_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY6077995.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2250 a         1355 c         1385 g         2195 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DADLEE010000158         7183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000158 DADLEE010000000
VERSION     DADLEE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.161"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(296..1198)
                     /locus_tag="JQH51_003829"
     CDS             complement(296..1198)
                     /locus_tag="JQH51_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY6077996.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQH51_003830"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQH51_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY6077997.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JQH51_003831"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JQH51_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY6077998.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JQH51_003832"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JQH51_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY6077999.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JQH51_003833"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JQH51_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY6078000.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JQH51_003834"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JQH51_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DADLEE010000159         7159 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000159 DADLEE010000000
VERSION     DADLEE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.162"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1076
                     /locus_tag="JQH51_003835"
     CDS             <1..1076
                     /locus_tag="JQH51_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY6078001.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="JQH51_003836"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="JQH51_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY6078002.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="JQH51_003837"
     CDS             complement(1623..1727)
                     /locus_tag="JQH51_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078003.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="JQH51_003838"
     CDS             1897..2343
                     /locus_tag="JQH51_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY6078004.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..2939)
                     /locus_tag="JQH51_003839"
                     /pseudo
     CDS             complement(2300..2939)
                     /locus_tag="JQH51_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="JQH51_003840"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="JQH51_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="JQH51_003841"
     CDS             4450..4797
                     /locus_tag="JQH51_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY6078005.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="JQH51_003842"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="JQH51_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY6078006.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="JQH51_003843"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="JQH51_003843"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY6078007.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /gene="yoaK"
                     /locus_tag="JQH51_003844"
     CDS             6314..6412
                     /gene="yoaK"
                     /locus_tag="JQH51_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY6078008.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="JQH51_003845"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="JQH51_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY6078009.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6841)
                     /locus_tag="JQH51_003846"
                     /pseudo
     CDS             complement(6650..6841)
                     /locus_tag="JQH51_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7021..>7159)
                     /locus_tag="JQH51_003847"
     CDS             complement(7021..>7159)
                     /locus_tag="JQH51_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078010.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
BASE COUNT         1727 a         1743 c         1743 g         1946 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc tgtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg cggcgaatac
     6961 tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt acccaatggg
     7021 ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt ccatatccaa
     7081 atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt tacgcatagt
     7141 gtcaggcttt aagcctgcc
//
LOCUS       DADLEE010000160         7080 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000160 DADLEE010000000
VERSION     DADLEE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.163"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..209)
                     /locus_tag="JQH51_003848"
     CDS             complement(<1..209)
                     /locus_tag="JQH51_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6078011.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JQH51_003849"
     tRNA            complement(324..399)
                     /locus_tag="JQH51_003849"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JQH51_003850"
     tRNA            complement(406..480)
                     /locus_tag="JQH51_003850"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JQH51_003851"
     tRNA            complement(597..681)
                     /locus_tag="JQH51_003851"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JQH51_003852"
     tRNA            complement(690..765)
                     /locus_tag="JQH51_003852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JQH51_003853"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JQH51_003853"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY6078012.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH51_003854"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQH51_003854"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY6078013.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH51_003855"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQH51_003855"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY6078014.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="JQH51_003856"
     rRNA            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="JQH51_003856"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4424..>7080)
                     /locus_tag="JQH51_003857"
     rRNA            complement(4424..>7080)
                     /locus_tag="JQH51_003857"
                     /product="23S ribosomal RNA"
BASE COUNT         1674 a         1949 c         1570 g         1887 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt ctgcttaatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc
     4261 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
     4321 accgcgctac ggccgccagg ctaattctgt tttatcagac cgcttctgcg ttctgattta
     4381 atctgtatca ggctgaaaat cttctctcaa tccgccaaaa catcttcggc gttgtaaggt
     4441 taagcctcac ggttcattag taccggttag ctcaacgcat cgctgcgctt acacacccgg
     4501 cctatcaacg tcgtcgtctt caacgttcct tcaggaccct taaagggtca gggagaactc
     4561 atctcggggc aagtttcgtg cttagatgct ttcagcactt atctcttccg catttagcta
     4621 ccgggcagtg ccattggcat gacaacccga acaccagtga tgcgtccact ccggtcctct
     4681 cgtactagga gcagcccccc tcagttctcc agcgcccacg gcagataggg accgaactgt
     4741 ctcacgacgt tctaaaccca gctcgcgtac cactttaaat ggcgaacagc catacccttg
     4801 ggacctactt cagccccagg atgtgatgag ccgacatcga ggtgccaaac accgccgtcg
     4861 atatgaactc ttgggcggta tcagcctgtt atccccggag taccttttat ccgttgagcg
     4921 atggcccttc cattcagaac caccggatca ctatgacctg ctttcgcacc tgctcgcgcc
     4981 gtcacgctcg cagtcaagct ggcttatgcc attgcactaa cctcctgatg tccgaccagg
     5041 attagccaac cttcgtgctc ctccgttact ctttaggagg agaccgcccc agtcaaacta
     5101 cccaccagac actgtccgca acccggatca cgggtccacg ttagaacatc aaacattaaa
     5161 gggtggtatt tcaaggtcgg ctccatgcag actggcgtcc acacttcaaa gcctcccacc
     5221 tatcctacac atcaaggctc aatgttcagt gtcaagctat agtaaaggtt cacggggtct
     5281 ttccgtcttg ccgcgggtac actgcatctt cacagcgagt tcaatttcac tgagtctcgg
     5341 gtggagacag cctggccatc attacgccat tcgtgcaggt cggaacttac ccgacaagga
     5401 atttcgctac cttaggaccg ttatagttac ggccgccgtt taccggggct tcgatcaaga
     5461 gcttcgcttg cgctaacccc atcaattaac cttccggcac cgggcaggcg tcacaccgta
     5521 tacgtccact ttcgtgtttg cacagtgctg tgtttttaat aaacagttgc agccagctgg
     5581 tatcttcgac tgatttcagc tccacgagca agtcgcttca cctacgtatc agcgtgcctt
     5641 ctcccgaagt tacggcacca ttttgcctag ttccttcacc cgagttctct caagcgcctt
     5701 ggtattctct acctgaccac ctgtgtcggt ttggggtacg atttgatgtt acctgatgct
     5761 tagaggcttt tcctggaagc agggcatttg ttgcttcagc accgtagtgc ctcgtcatca
     5821 cgcctcggcc ttgattttcc ggatttgcct ggaaaaccag cctacacgct taaaccggga
     5881 caaccgtcgc ccggccaaca tagccttctc cgtcccccct tcgcagtaac accaagtaca
     5941 ggaatattaa cctgtttccc atcgactacg cctttcggcc tcgccttagg ggtcgactca
     6001 ccctgccccg attaacgttg gacaggaacc cttggtcttc cggcgagcgg gcttttcacc
     6061 cgctttatcg ttacttatgt cagcattcgc acttctgata cctccagcat acctcacagc
     6121 acaccttcac aggcttacag aacgctcccc tacccaacaa cgcataagcg tcgctgccgc
     6181 agcttcggtg catggtttag ccccgttaca tcttccgcgc aggccgactc gaccagtgag
     6241 ctattacgct ttctttaaat gatggctgct tctaagccaa catcctggct gtctgggcct
     6301 tcccacatcg tttcccactt aaccatgact ttgggacctt agctggcggt ctgggttgtt
     6361 tccctcttca cgacggacgt tagcacccgc cgtgtgtctc ccgtgataac attctccggt
     6421 attcgcagtt tgcatcgggt tggtaagtcg ggatgacccc cttgccgaaa cagtgctcta
     6481 cccccggaga tgaattcacg aggcgctacc taaatagctt tcggggagaa ccagctatct
     6541 cccggtttga ttggcctttc acccccagcc acaagtcatc cgctaatttt tcaacattag
     6601 tcggttcggt cctccagtta gtgttaccca accttcaacc tgcccatggc tagatcaccg
     6661 ggtttcgggt ctataccctg caacttaacg cccagttaag actcggtttc ccttcggctc
     6721 ccctattcgg ttaaccttgc tacagaatat aagtcgctga cccattatac aaaaggtacg
     6781 cagtcacacg cctaagcgtg ctcccactgc ttgtacgtac acggtttcag gttctttttc
     6841 actcccctcg ccggggttct tttcgccttt ccctcacggt actggttcac tatcggtcag
     6901 tcaggagtat ttagccttgg aggatggtcc ccccatattc agacaggata ccacgtgtcc
     6961 cgccctactc atcgagctca cagtatgtgc atttttgtgt acggggctgt caccctgtat
     7021 cgcacgcctt tccagacgct tccactaaca cacacactga ttcaggctct gggctgctcc
//
LOCUS       DADLEE010000161         6910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000161 DADLEE010000000
VERSION     DADLEE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.165"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..694
                     /locus_tag="JQH51_003858"
     CDS             <1..694
                     /locus_tag="JQH51_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY6078015.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="JQH51_003859"
     CDS             complement(1033..1803)
                     /locus_tag="JQH51_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078016.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="JQH51_003860"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="JQH51_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="JQH51_003861"
     CDS             complement(2294..2515)
                     /locus_tag="JQH51_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078017.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="JQH51_003862"
     CDS             complement(2516..3199)
                     /locus_tag="JQH51_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY6078018.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="JQH51_003863"
     CDS             complement(3263..3574)
                     /locus_tag="JQH51_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078019.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4542)
                     /locus_tag="JQH51_003864"
     CDS             complement(3571..4542)
                     /locus_tag="JQH51_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY6078020.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVNVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="JQH51_003865"
     CDS             4913..5872
                     /locus_tag="JQH51_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY6078021.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="JQH51_003866"
     CDS             5872..6267
                     /locus_tag="JQH51_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY6078022.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="JQH51_003867"
     CDS             6450..6830
                     /locus_tag="JQH51_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY6078023.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1889 a         1786 c         1674 g         1561 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttaccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DADLEE010000162         6795 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000162 DADLEE010000000
VERSION     DADLEE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.166"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            240..2672
                     /locus_tag="JQH51_003868"
     CDS             240..2672
                     /locus_tag="JQH51_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY6078024.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2703..4208
                     /locus_tag="JQH51_003869"
     CDS             2703..4208
                     /locus_tag="JQH51_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY6078025.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="JQH51_003870"
     CDS             4208..5869
                     /locus_tag="JQH51_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY6078026.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="JQH51_003871"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="JQH51_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY6078027.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="JQH51_003872"
     CDS             complement(6478..>6795)
                     /locus_tag="JQH51_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078028.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DADLEE010000163         6606 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000163 DADLEE010000000
VERSION     DADLEE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.167"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..357
                     /locus_tag="JQH51_003873"
     CDS             <1..357
                     /locus_tag="JQH51_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6078029.1"
                     /translation="VAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            727..1551
                     /gene="gadX"
                     /locus_tag="JQH51_003874"
     CDS             727..1551
                     /gene="gadX"
                     /locus_tag="JQH51_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY6078030.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1919..2647
                     /gene="gadW"
                     /locus_tag="JQH51_003875"
     CDS             1919..2647
                     /gene="gadW"
                     /locus_tag="JQH51_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY6078031.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3010..6123)
                     /locus_tag="JQH51_003876"
                     /pseudo
     CDS             complement(3010..6123)
                     /locus_tag="JQH51_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(6148..6405)
                     /locus_tag="JQH51_003877"
                     /pseudo
     CDS             complement(6148..6405)
                     /locus_tag="JQH51_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT         1739 a         1551 c         1568 g         1748 t
ORIGIN      
        1 gttgccgctt atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg
       61 ggtcgcccgg acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg
      121 ggatacaccc tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
      181 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
      241 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
      301 agcgatcacc cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa
      361 cataacgttg taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct
      421 tattaatcgc aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat
      481 gaatgtttat taaatgtagc gattaccttt aatgtcacga aaagggatta tttgcttgct
      541 attaatttca ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt
      601 agaacatcac acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat
      661 ttgacttaag agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag
      721 tcaactatgc aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt
      781 ctcaccatgg ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat
      841 gcaagccaaa ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg
      901 ctttcattat ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa
      961 atttcttcat tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg
     1021 caactatcgc agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc
     1081 tttgcgttgt tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt
     1141 ttgcaaccga acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag
     1201 tggacactag cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa
     1261 ttgcgcgaag aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct
     1321 ttgcaactta ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat
     1381 cacagcgtgt cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag
     1441 tatcaggagc gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg
     1501 caagggaatt tttacggcac taaccgttct gcggaaggaa taagattata gagttttact
     1561 cagacataaa aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac
     1621 taataaaccg ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa
     1681 atataacttt tacaggaaat aagatcagcc atttttttat aaacataagc tatacgctgt
     1741 gcgaaaatat attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt
     1801 tgtggataaa ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca
     1861 tcatttcatt agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat
     1921 gactcatgtc tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca
     1981 aaaaatatcg ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt
     2041 tgcgttttgt tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta
     2101 cttacaaaat attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt
     2161 aattaaccag tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc
     2221 cggggtaaat tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc
     2281 tcataagaaa gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt
     2341 tgaacgcctt attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag
     2401 aatgtatacc agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag
     2461 taaaatatta ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat
     2521 tcctctgcat actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac
     2581 atttcgacaa tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt
     2641 ttcctgacat attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac
     2701 tcttccctga tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg
     2761 tataggtatt tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca
     2821 tacgattacg ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag
     2881 tggtttctac tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt
     2941 gagtggtgaa tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc
     3001 atttacacgt tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac
     3061 cggaacgaag taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc
     3121 taccgcgttt tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag
     3181 aataaaggcc agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat
     3241 tgcctctatc ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag
     3301 gatggcgttc ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc
     3361 attacttaag ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac
     3421 caacatcacc gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac
     3481 cacgaccagt gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata
     3541 cgacagtccg gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat
     3601 aaatttcata gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga
     3661 cgggatgccg ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta
     3721 ggcagaaagc ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc
     3781 cggcaacata cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt
     3841 gaggaagtcg ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga
     3901 cagcgccaca cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt
     3961 atcttccagg ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga
     4021 taacagctcg tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag
     4081 ttccatatca aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa
     4141 gacgacggct ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac
     4201 cgagttttcc tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt
     4261 gttttgcccc tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc
     4321 tttctctttt gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat
     4381 ggtggcaccg gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa
     4441 gaaagaggtc ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac
     4501 caccatgtag cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt
     4561 tgatttttca aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc
     4621 cggcgtggct ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac
     4681 tgaaagccgc atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc
     4741 accgctcata aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag
     4801 cgcacgttgg atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc
     4861 cgcaatgaca cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat
     4921 cgccagcacc atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat
     4981 cgcaaacgtc ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa
     5041 attctgcaaa aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt
     5101 gaaaacttcc tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt
     5161 cagacttgcg gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt
     5221 atccagggcg tttgctccgg cagccagttt gatggcgatc ccggcaacag gtttgccgtt
     5281 atagcgcgcc acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag
     5341 cagcacttgc gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt
     5401 ttgcagacgc gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat
     5461 gccacccagt tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc
     5521 ggaaggtacc aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta
     5581 ctcggaacca aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat
     5641 attagacgct acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa
     5701 cgccgctacc atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac
     5761 cgcttcaggt aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat
     5821 atcaggagat gccccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact
     5881 ggttgaagac atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt
     5941 gaccgagtct tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt
     6001 cggtggcgca atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc
     6061 aagcatcata ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc
     6121 catgaaagtc cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg
     6181 gctggaggaa attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat
     6241 cacccgatcg ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct
     6301 ggcttcaatt tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt
     6361 accctgggcg ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt
     6421 attcaagata tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt
     6481 caagataaaa atgcttgtta attaacaaat gatatcttct atatattttt attgtttata
     6541 gatggctatt tcggtaaaaa tgcattttca gaatatcaac cagcggtaaa tcgaagtaaa
     6601 gggagt
//
LOCUS       DADLEE010000164         6520 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000164 DADLEE010000000
VERSION     DADLEE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.168"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            439..1827
                     /locus_tag="JQH51_003878"
     CDS             439..1827
                     /locus_tag="JQH51_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAY6078032.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /locus_tag="JQH51_003879"
                     /pseudo
     CDS             1909..5649
                     /locus_tag="JQH51_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="JQH51_003880"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="JQH51_003880"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY6078033.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DADLEE010000165         6321 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000165 DADLEE010000000
VERSION     DADLEE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.169"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            73..378
                     /locus_tag="JQH51_003881"
     CDS             73..378
                     /locus_tag="JQH51_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078034.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="JQH51_003882"
     CDS             complement(454..918)
                     /locus_tag="JQH51_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY6078035.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /locus_tag="JQH51_003883"
     CDS             complement(915..1790)
                     /locus_tag="JQH51_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY6078036.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="JQH51_003884"
     CDS             complement(1787..2476)
                     /locus_tag="JQH51_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY6078037.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JQH51_003885"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JQH51_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY6078038.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JQH51_003886"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JQH51_003886"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY6078039.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLATLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JQH51_003887"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JQH51_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY6078040.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta gccagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DADLEE010000166         6161 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000166 DADLEE010000000
VERSION     DADLEE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.170"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..171
                     /locus_tag="JQH51_003888"
     CDS             <1..171
                     /locus_tag="JQH51_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6078041.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /locus_tag="JQH51_003889"
     CDS             276..1628
                     /locus_tag="JQH51_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY6078042.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="JQH51_003890"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="JQH51_003890"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY6078043.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="JQH51_003891"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="JQH51_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY6078044.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="JQH51_003892"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="JQH51_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY6078045.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6161
                     /locus_tag="JQH51_003893"
                     /pseudo
     CDS             5430..6161
                     /locus_tag="JQH51_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1579 a         1311 c         1558 g         1713 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac gatctgttta a
//
LOCUS       DADLEE010000167         6058 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000167 DADLEE010000000
VERSION     DADLEE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.171"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..143)
                     /locus_tag="JQH51_003894"
     CDS             complement(<1..143)
                     /locus_tag="JQH51_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HAY6078046.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="JQH51_003895"
     CDS             379..651
                     /locus_tag="JQH51_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY6078047.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="JQH51_003896"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="JQH51_003896"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY6078048.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="JQH51_003897"
     CDS             1111..1731
                     /locus_tag="JQH51_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY6078049.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /locus_tag="JQH51_003898"
                     /pseudo
     CDS             complement(1740..3047)
                     /locus_tag="JQH51_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(3114..3767)
                     /locus_tag="JQH51_003899"
     CDS             complement(3114..3767)
                     /locus_tag="JQH51_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY6078050.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="JQH51_003900"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="JQH51_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="JQH51_003901"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="JQH51_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DADLEE010000168         6052 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000168 DADLEE010000000
VERSION     DADLEE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.172"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            430..1338
                     /locus_tag="JQH51_003902"
     CDS             430..1338
                     /locus_tag="JQH51_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY6078051.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQH51_003903"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQH51_003903"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY6078052.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /locus_tag="JQH51_003904"
     CDS             3653..4411
                     /locus_tag="JQH51_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY6078053.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQH51_003905"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQH51_003905"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY6078054.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="JQH51_003906"
     CDS             complement(5673..>6052)
                     /locus_tag="JQH51_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078055.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DADLEE010000169         5982 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000169 DADLEE010000000
VERSION     DADLEE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.173"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..392)
                     /locus_tag="JQH51_003907"
                     /pseudo
     CDS             complement(1..392)
                     /locus_tag="JQH51_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="JQH51_003908"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="JQH51_003908"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY6078056.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="JQH51_003909"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="JQH51_003909"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY6078057.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5796..>5982
                     /locus_tag="JQH51_003910"
     CDS             5796..>5982
                     /locus_tag="JQH51_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078058.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1769 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggagt agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca
     4561 cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 ctgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac
     5881 aatgggcagc gatttgttcc attgccccaa agattggctg tatactggaa actctgcgtg
     5941 tctgggtacg ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DADLEE010000170         5889 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000170 DADLEE010000000
VERSION     DADLEE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.174"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            50..244
                     /locus_tag="JQH51_003911"
     CDS             50..244
                     /locus_tag="JQH51_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY6078059.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="JQH51_003912"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="JQH51_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6078060.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="JQH51_003913"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="JQH51_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY6078061.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="JQH51_003914"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="JQH51_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY6078062.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="JQH51_003915"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="JQH51_003915"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY6078063.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="JQH51_003916"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="JQH51_003916"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY6078064.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5864
                     /locus_tag="JQH51_003917"
     CDS             5493..5864
                     /locus_tag="JQH51_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HAY6078065.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTD"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DADLEE010000171         5805 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000171 DADLEE010000000
VERSION     DADLEE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.175"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JQH51_003918"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JQH51_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY6078066.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JQH51_003919"
     CDS             578..1027
                     /locus_tag="JQH51_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY6078067.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="JQH51_003920"
     CDS             complement(1028..1261)
                     /locus_tag="JQH51_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY6078068.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /locus_tag="JQH51_003921"
     CDS             complement(1347..1520)
                     /locus_tag="JQH51_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY6078069.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="JQH51_003922"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="JQH51_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY6078070.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="JQH51_003923"
     CDS             complement(2212..3021)
                     /locus_tag="JQH51_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY6078071.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQH51_003924"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQH51_003924"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAY6078072.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="JQH51_003925"
     CDS             complement(4677..5471)
                     /locus_tag="JQH51_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY6078073.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JQH51_003926"
     CDS             complement(5461..>5805)
                     /locus_tag="JQH51_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY6078074.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1474 c         1287 g         1522 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagaaacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLEE010000172         5743 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000172 DADLEE010000000
VERSION     DADLEE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.176"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..172
                     /locus_tag="JQH51_003927"
                     /pseudo
     CDS             1..172
                     /locus_tag="JQH51_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(229..647)
                     /locus_tag="JQH51_003928"
                     /pseudo
     CDS             complement(229..647)
                     /locus_tag="JQH51_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            697..1875
                     /gene="ltrA"
                     /locus_tag="JQH51_003929"
     CDS             697..1875
                     /gene="ltrA"
                     /locus_tag="JQH51_003929"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY6078075.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1958..2137
                     /locus_tag="JQH51_003930"
                     /pseudo
     CDS             1958..2137
                     /locus_tag="JQH51_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2167..2649)
                     /locus_tag="JQH51_003931"
                     /pseudo
     CDS             complement(2167..2649)
                     /locus_tag="JQH51_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2905..3885)
                     /locus_tag="JQH51_003932"
     CDS             complement(2905..3885)
                     /locus_tag="JQH51_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY6078076.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3885..5084)
                     /locus_tag="JQH51_003933"
     CDS             complement(3885..5084)
                     /locus_tag="JQH51_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY6078077.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5406..>5743)
                     /locus_tag="JQH51_003934"
     CDS             complement(5406..>5743)
                     /locus_tag="JQH51_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY6078078.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1484 t
ORIGIN      
        1 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
       61 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
      121 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata
      181 agttatccat gctctgaggt gggtagaggt gggccctccg gtcagggttt acaggagcac
      241 agaccaaacg acccggtgct gcttcattct aaggaatgag gtggtgagcg actcgggaaa
      301 aggcagagat ctactgtcac gtgggttcgg gaagctgaac gaaagtaaac tgtccgatga
      361 agcatcgaaa tcttagatga tgccaaaatg gggccgtaca gccgtcccct gataagcgca
      421 gaagacacct gcttactgac tgcgcggcat ccggtgtgaa ggcagcagga tcccgggcca
      481 gaccatctca gggaacgtga gaacctgttt ccggcacgta tcgggaaaca ggggcggacc
      541 cattcgtagt agtgatgaaa gatctgtaat gggtctggag cgaagggatg gggatcacat
      601 tcattgcaga agacacaacc caaaagggga tgacgtaact gatgcaacga aaatcatttg
      661 aaataccaaa agcactggtc tgggcatcat atctggatgt gcgtcgcaac aagggagccc
      721 cccggatgcg acgggcagac tttgaaaatg ttcgaccagc aacgtgatgg caatctatac
      781 aagatctgga accggctatg ttcgggaaca tggtttccgc caccagtgct ggaaaaacgg
      841 atcccgaagt cgaatggcaa ggaacgcatt ctggggatcc caacagtatc cgatcgaatt
      901 gctcagggag cgataaaact tttcatggaa gaaaagcttg acccgatttt ccatgctgat
      961 tcatatggtt accgaccagg caaatcagct catgacgctc tgaaacaatg tgccatccgg
     1021 tgctggcgtt atagctggat actggaagtg gacatcagtg cgtttttcga ccatgtgaga
     1081 catgatctgg tgctcaaagc tctggaacac cacgggatgc ctaaatgggt catcctgtac
     1141 tgtcgacgct ggatggaagc gccaatgcag agttgtgaaa atggagaatt aataacccga
     1201 acacgaggca ctccgcaggg cggggtcatt agtccgctac ttgctaactt gttccttcac
     1261 tatgcatttg atttgtggat ggaaagagaa tatcgggggg taccgtttga gaggtacgct
     1321 gacgatattg tagtacattg ttcacgaatg agtgatgcaa caaggctaaa gaacaggtta
     1381 tcggagcgct tctcggaagt cgggctggtc ctgaatgcag ggaaaacgaa tatcgcctac
     1441 attgacacgt ttaaaaggag aaacgtcgca acgagtttca cctttctcgg atatgacttc
     1501 aaagtgcgta cgctgaagaa tttcaacggc gaacggtacc gaaaatgcat gccgggtgcg
     1561 tcaaatgcag caatgcgcaa aataacagaa acaatcaaga agtggcgtat acatcgctca
     1621 acagctgaga gtttgctgga ttttgcgagg cgctacaatg cgatagtgag aggctggatc
     1681 gagtactacg gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag
     1741 tcacgtctgc tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga
     1801 aagctgacgc tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt
     1861 gcatcaaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc
     1921 agccggggag tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga
     1981 acatgcggcg gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga
     2041 aaaatggctg cgttacgtca ttgagcatat ccaggactgg ccggcaaacc gggtacgtga
     2101 tctgttgccc tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc
     2161 cgcttacttt tggcgtaatt ctgctgcgcc tttactgaac acaatgccgt attcagcaag
     2221 aagtccacga atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt
     2281 caaaaaagtc tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc
     2341 atgttggcag gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg
     2401 ataagcttta acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc
     2461 ccgggcccag taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg
     2521 caccagccag ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg
     2581 agcattgata ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg
     2641 ttcagacatg gtatcccgct tacttttgtt gttaaagttt atccatcatg gcgcaattga
     2701 aggctgccgt gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa
     2761 actatctttt tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt
     2821 tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggcga
     2881 gttgctggcg aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc
     2941 aatcatacga tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa
     3001 cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa
     3061 agttacaacc gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat
     3121 aagccgcata atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag
     3181 ctcaatactc ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag
     3241 tcgttcactt aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg
     3301 aaaaagagac acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc
     3361 tgccgacaat ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg
     3421 tactaaacgc aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc
     3481 cagatgctga gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac
     3541 cttgcatagc aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc
     3601 agtccgaatc ccaatacatg gatagaattg ttgtaaacgt atagttctgg tgatgtcttt
     3661 tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt
     3721 ttgagtatcc actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt
     3781 attgagggta aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt
     3841 attcagcgta cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt
     3901 ataaattcaa tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt
     3961 ttcaaagctt caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac
     4021 gactcgccgc ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca
     4081 ccgaaaatct cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc
     4141 gacatgaagc caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt
     4201 acaagttcag gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc
     4261 ggcaccggtg taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga
     4321 tgcgggcctg tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc
     4381 tcacgcaata tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag
     4441 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
     4501 gacggaacga taaaatcttc cagtaattcc tcacgactca cgttctgcaa catagcctgc
     4561 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
     4621 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
     4681 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
     4741 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
     4801 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
     4861 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
     4921 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
     4981 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
     5041 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
     5101 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
     5161 attgtacatg cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat
     5221 attactgtaa gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca
     5281 gttcaccacc acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg
     5341 aaacatgttc cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct
     5401 tccaatcagt agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc
     5461 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc
     5521 cggttgttac gtgcaatacg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc
     5581 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc
     5641 aacagggcta acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac
     5701 acaccgccag ttttagtgtt gtacgcatac cagagccagt gtt
//
LOCUS       DADLEE010000173         5738 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000173 DADLEE010000000
VERSION     DADLEE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.177"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..241
                     /locus_tag="JQH51_003935"
     CDS             <1..241
                     /locus_tag="JQH51_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078079.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="JQH51_003936"
     CDS             complement(238..1179)
                     /locus_tag="JQH51_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY6078080.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1324..1680)
                     /locus_tag="JQH51_003937"
     CDS             complement(1324..1680)
                     /locus_tag="JQH51_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078081.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDLH"
     gene            complement(1684..2904)
                     /locus_tag="JQH51_003938"
     CDS             complement(1684..2904)
                     /locus_tag="JQH51_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY6078082.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="JQH51_003939"
     CDS             complement(2908..3651)
                     /locus_tag="JQH51_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY6078083.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="JQH51_003940"
     CDS             complement(3542..5008)
                     /locus_tag="JQH51_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY6078084.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="JQH51_003941"
     CDS             complement(5008..5241)
                     /locus_tag="JQH51_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY6078085.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="JQH51_003942"
     CDS             complement(5229..5708)
                     /locus_tag="JQH51_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078086.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1226 a         1618 c         1434 g         1460 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc catcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DADLEE010000174         5702 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000174 DADLEE010000000
VERSION     DADLEE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.178"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..170)
                     /locus_tag="JQH51_003943"
     CDS             complement(<1..170)
                     /locus_tag="JQH51_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078087.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="JQH51_003944"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="JQH51_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY6078088.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="JQH51_003945"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="JQH51_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4413)
                     /locus_tag="JQH51_003946"
     CDS             complement(3616..4413)
                     /locus_tag="JQH51_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY6078089.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="JQH51_003947"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="JQH51_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="JQH51_003948"
     CDS             5484..>5702
                     /locus_tag="JQH51_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY6078090.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DADLEE010000175         5600 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000175 DADLEE010000000
VERSION     DADLEE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.179"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..410
                     /locus_tag="JQH51_003949"
     CDS             <1..410
                     /locus_tag="JQH51_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6078091.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JQH51_003950"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JQH51_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JQH51_003951"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JQH51_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY6078092.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1332 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tctacagcgc aggagagctg
      721 ttgcaggaac tacgtaaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag acctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggcata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggac ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gaccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc ggcgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaggaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tactcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatcg
//
LOCUS       DADLEE010000176         5590 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000176 DADLEE010000000
VERSION     DADLEE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.180"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..279)
                     /locus_tag="JQH51_003952"
     CDS             complement(1..279)
                     /locus_tag="JQH51_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078093.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="JQH51_003953"
     CDS             220..405
                     /locus_tag="JQH51_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078094.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..659)
                     /locus_tag="JQH51_003954"
     CDS             complement(447..659)
                     /locus_tag="JQH51_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6078095.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(862..1080)
                     /locus_tag="JQH51_003955"
     CDS             complement(862..1080)
                     /locus_tag="JQH51_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078096.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(1254..1589)
                     /locus_tag="JQH51_003956"
     CDS             complement(1254..1589)
                     /locus_tag="JQH51_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078097.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
     gene            1697..1837
                     /locus_tag="JQH51_003957"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="JQH51_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="JQH51_003958"
     CDS             1837..2862
                     /locus_tag="JQH51_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6078098.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="JQH51_003959"
     tRNA            complement(2915..3004)
                     /locus_tag="JQH51_003959"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="JQH51_003960"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="JQH51_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY6078099.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="JQH51_003961"
     tRNA            3996..4071
                     /locus_tag="JQH51_003961"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="JQH51_003962"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="JQH51_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DADLEE010000177         5557 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000177 DADLEE010000000
VERSION     DADLEE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.181"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..641
                     /locus_tag="JQH51_003963"
     CDS             <1..641
                     /locus_tag="JQH51_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078100.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQH51_003964"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQH51_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY6078101.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="JQH51_003965"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="JQH51_003965"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY6078102.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DADLEE010000178         5485 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000178 DADLEE010000000
VERSION     DADLEE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.182"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1630)
                     /locus_tag="JQH51_003966"
     CDS             complement(<1..1630)
                     /locus_tag="JQH51_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY6078103.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="JQH51_003967"
     CDS             complement(1646..2530)
                     /locus_tag="JQH51_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY6078104.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="JQH51_003968"
     CDS             complement(2541..3146)
                     /locus_tag="JQH51_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY6078105.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="JQH51_003969"
     CDS             complement(3317..>5485)
                     /locus_tag="JQH51_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HAY6078106.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DADLEE010000179         5400 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000179 DADLEE010000000
VERSION     DADLEE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.183"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..497
                     /locus_tag="JQH51_003970"
     CDS             <1..497
                     /locus_tag="JQH51_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY6078107.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="JQH51_003971"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="JQH51_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="JQH51_003972"
     CDS             2500..3114
                     /locus_tag="JQH51_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY6078108.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="JQH51_003973"
     CDS             3114..3443
                     /locus_tag="JQH51_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY6078109.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="JQH51_003974"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="JQH51_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY6078110.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="JQH51_003975"
     CDS             4529..4840
                     /locus_tag="JQH51_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY6078111.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="JQH51_003976"
     CDS             4824..4982
                     /locus_tag="JQH51_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY6078112.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="JQH51_003977"
     CDS             4975..5373
                     /locus_tag="JQH51_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6078113.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1178 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DADLEE010000180         5339 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000180 DADLEE010000000
VERSION     DADLEE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.184"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="JQH51_003978"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="JQH51_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY6078114.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="JQH51_003979"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="JQH51_003979"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY6078115.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="JQH51_003980"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="JQH51_003980"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY6078116.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="JQH51_003981"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="JQH51_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY6078117.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1457 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DADLEE010000181         5182 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000181 DADLEE010000000
VERSION     DADLEE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.185"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            9..746
                     /locus_tag="JQH51_003982"
     CDS             9..746
                     /locus_tag="JQH51_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY6078118.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JQH51_003983"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JQH51_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY6078119.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3606..3809
                     /gene="safA"
                     /locus_tag="JQH51_003984"
     CDS             3606..3809
                     /gene="safA"
                     /locus_tag="JQH51_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY6078120.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3877..4638
                     /gene="ydeO"
                     /locus_tag="JQH51_003985"
     CDS             3877..4638
                     /gene="ydeO"
                     /locus_tag="JQH51_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY6078121.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1480 a         1103 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa
     3661 tcatgtctgt aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg
     3721 caattgatca gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca
     3781 ttatcaatag tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa
     3841 ggtatcgata aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata
     3901 tttattcatc atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc
     3961 gagatcctga tggtagataa cgctgttcgt acacattttg aaccttatga gcggcatttt
     4021 aaagaaatcg gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag
     4081 acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg
     4141 cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa
     4201 cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta
     4261 actaacggtg tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg
     4321 cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag
     4381 aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag
     4441 cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt
     4501 tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag
     4561 agagtttcta aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg
     4621 agtgctttag ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa
     4681 ttatatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata
     4741 aataagtgac atccatcaca tatttatgca cttgcataac ctgttgcatg attatttatg
     4801 atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt
     4861 tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg
     4921 ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc
     4981 cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta
     5041 tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg
     5101 gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa
     5161 acgttgcttt ctcagacttt ac
//
LOCUS       DADLEE010000182         5162 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000182 DADLEE010000000
VERSION     DADLEE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.186"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..220
                     /locus_tag="JQH51_003986"
     CDS             <1..220
                     /locus_tag="JQH51_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6078122.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="JQH51_003987"
     CDS             complement(392..721)
                     /locus_tag="JQH51_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY6078123.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="JQH51_003988"
     CDS             complement(726..911)
                     /locus_tag="JQH51_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY6078124.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="JQH51_003989"
     CDS             complement(908..3547)
                     /locus_tag="JQH51_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY6078125.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="JQH51_003990"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="JQH51_003990"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY6078126.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5162
                     /gene="hslJ"
                     /locus_tag="JQH51_003991"
     CDS             4855..>5162
                     /gene="hslJ"
                     /locus_tag="JQH51_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY6078127.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1307 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aa
//
LOCUS       DADLEE010000183         5151 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000183 DADLEE010000000
VERSION     DADLEE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.187"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            190..447
                     /locus_tag="JQH51_003992"
                     /pseudo
     CDS             190..447
                     /locus_tag="JQH51_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JQH51_003993"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JQH51_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY6078128.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JQH51_003994"
     CDS             complement(1599..2351)
                     /locus_tag="JQH51_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY6078129.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JQH51_003995"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JQH51_003995"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY6078130.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JQH51_003996"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JQH51_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..4420)
                     /locus_tag="JQH51_003997"
     CDS             complement(3605..4420)
                     /locus_tag="JQH51_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY6078131.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTCYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="JQH51_003998"
     CDS             4517..>5151
                     /locus_tag="JQH51_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY6078132.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1226 g         1376 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcacgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADLEE010000184         5138 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000184 DADLEE010000000
VERSION     DADLEE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.188"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            227..483
                     /locus_tag="JQH51_003999"
                     /pseudo
     CDS             227..483
                     /locus_tag="JQH51_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421719.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            626..847
                     /locus_tag="JQH51_004000"
                     /pseudo
     CDS             626..847
                     /locus_tag="JQH51_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(868..930)
                     /locus_tag="JQH51_004001"
                     /pseudo
     CDS             complement(868..930)
                     /locus_tag="JQH51_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1380..2792
                     /gene="ospC1"
                     /locus_tag="JQH51_004002"
     CDS             1380..2792
                     /gene="ospC1"
                     /locus_tag="JQH51_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY6078133.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2896..3045)
                     /locus_tag="JQH51_004003"
                     /pseudo
     CDS             complement(2896..3045)
                     /locus_tag="JQH51_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3121..4818
                     /gene="ospD3"
                     /locus_tag="JQH51_004004"
     CDS             3121..4818
                     /gene="ospD3"
                     /locus_tag="JQH51_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY6078134.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4935..5054
                     /locus_tag="JQH51_004005"
                     /pseudo
     CDS             4935..5054
                     /locus_tag="JQH51_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1740 a          958 c          982 g         1458 t
ORIGIN      
        1 gggctgcatc cgactctttt atcaacgctt acgtaattaa gggacacagc ctagtctcca
       61 ggattcccgg ggcggttcaa tcaaagcgca gaacttctgc gtcttcttcg tcaaaaaaat
      121 catcttcgtg aagcttcacg acatagcggg gaagtttgct tctttgagag gcgggtttac
      181 gtttacggga tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt
      241 ttttataacg gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc
      301 acaaggctct ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt
      361 acaccataag gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta
      421 catccaaatt gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta
      481 taaaggcttg agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc
      541 agcaatgaac tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc
      601 atataacccg ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct
      661 gcgggcactg ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct
      721 gctctcacag cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc
      781 gaaactgcag cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg
      841 gcagattcca ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc
      901 cattaaggcg attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat
      961 tatatatggc caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt
     1021 catgaaaaaa tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc
     1081 cataagagga atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa
     1141 aattgatata gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt
     1201 aagcatcgga ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa
     1261 tactcttatc taattacatg cagtatattg tatatatact caaaaattgt aattcatacc
     1321 ctgctatatc ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta
     1381 tgaatatatc agaaacactg aactcagcaa atacccaatg caatatagat tctatggata
     1441 acagattaca tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta
     1501 tgccagatga aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa
     1561 ctatagcagc acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa
     1621 atatagcaat tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg
     1681 acagattatc gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag
     1741 aggaactcaa tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta
     1801 attctgattt gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag
     1861 gaatatgcac aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact
     1921 ttgtgttttt tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac
     1981 atcacactgt tgattttggt gcaaatgcgt atatcattga tcatgactct ccatatggat
     2041 atatgacatt aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc
     2101 aatcattttt agataaattt tcagaggtta ataaagaagt tagtcgatac gtacatggaa
     2161 gtaaaggaat tatagatgta ccaatattca atactaaaga tatgaagtta gggctcggat
     2221 tatacctgat tgactttatt agaaaaagtg aagaccaaag cttcaaggag ttttgctatg
     2281 gaaaaaatct tgcccctgtg gatctggata gaatcataaa ctttgttttt cagccagagt
     2341 accatatacc taggatggta agtacagaaa acttcaaaaa agttaagatt agagaaatat
     2401 ccttagagga ggctgttaca gcatctaatt acgaagaaat taacaagcag gtcactaaca
     2461 aaaaaattgc tctccaggct ctttttcttt cgattactaa tcaaaaagag gatgtcgcct
     2521 tatatatatt atctaatttt gagataacta gacaagatgt tatttccata aagcatgagt
     2581 tgtatgatat tgagtatcta cttagcgctc ataattcaag ctgtaaagta cttgagtatt
     2641 ttatcaataa gggattggtt gatgtaaaca caaagttcaa aaaaactaat agtggggatt
     2701 gtatgttgga taacgcaata aaatatgaga atgcagaaat gataaaacta ttattgaaat
     2761 atggtgcaac atctgacaat aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg
     2821 cagtatttgc acaaaatatc gatatattgt ttattgtcag tatggctcaa tgtgataata
     2881 gttggaaagt ttgatgggtt tcgccccgtt gtagcggtag tcgaactgga aggtcttcag
     2941 gcactgctta cagcgataga gcagcccccc tgaactggaa tggccgttcc ggataccccc
     3001 ctgagtttca gagtaacggt gacaaaccac atcaatcttt gccatcaatc atccaaaggg
     3061 caaagagtac aacaacacta agtctgcgtc acaacccatc aatgaaagga atatatacat
     3121 atgccatcag taaatttaat cccatcaagg aaaatatgtt tgcaaaatat gataaataaa
     3181 gacaacgtct ctgttgagac aatccagtct ctattgcact caaaacaatt gccatatttt
     3241 tctgacaaga ggagtttttt attaaatcta aattgccaag ttaccgatca ctctggaaga
     3301 cttattgtct gtcgacattt agcttcctac tggatagcac agtttaacaa aagtagtggt
     3361 cacgtggatt atcatcactt tgcttttccg gatgaaatta aaaattatgt ttcagtgagt
     3421 gaagaagaaa aggctattaa tgtgcctgct attatttatt ttgttgaaaa cggttcatgg
     3481 ggagatatta ttttttatat tttcaatgaa atgatttttc attccgaaaa aagcagagca
     3541 ctagaaataa gtacatcaaa tcacaatatg gcattaggct tgaagattaa agaaactaaa
     3601 aatggggggg attttgtcat tcagctttat gatcccaacc atacagcaac tcatttacga
     3661 gcagagttta acaaatttaa cttagctaaa ataaaaaaac tgactgtaga taattttctt
     3721 gatgaaaaac atcagaaatg ttatggtctt atatccgacg gtatgtctat atttgtggac
     3781 agacatactc caacaagcat gtcctccata atcagatggc ctgataattt acttcacccc
     3841 aaagttattt atcacgcgat gcgtatggga ttgactgagc taatccaaaa agtaacaaga
     3901 gtcgtacaac tatctgacct ttcagacaat acgttagaat tacttttggc agccaaaaat
     3961 gacgatggtt tgtcaggatt gcttttagct ttacaaaatg ggcattcaga tacaatctta
     4021 gcatacggag aactcttgga aacttctgga cttaaccttg ataaaacggt agaactacta
     4081 actgcggaag gaatgggagg acgaatatcg ggtttatccc aagcacttca aaatgggcat
     4141 gcagaaacta tcaaaacata cggaaggctt ctcaagaaga gagcaataaa tatcgaatac
     4201 aataagctga aaaatttgct gaccgcttat tattatgatg aagtacacag acagataccc
     4261 ggactaatgt ttgctcttca aaatggacat gcagatgcta tacgcgcata cggtgagctc
     4321 attcttagcc cccctctcct caactcagag gatattgtaa atttgctggc ctcaaggaga
     4381 tatgacaatg ttcccggact tctgttagca ttgaataatg gacaggctga tgcaatctta
     4441 gcttatggtg atatcttgaa tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta
     4501 gaagcgaaag attctaatgg tttatctgga ttgtttgtag ccttgcataa tggatgtgta
     4561 gaaacaatta ttgcttatgg gaaaatactt cacactgcag accttactcc acatcaggca
     4621 tcaaaattac tggcagcaga aggcccaaat ggggtatctg gattaattat agcttttcaa
     4681 aataggaatt ttgaggcaat aaaaacttat atggaaataa taaaaaatga aaatattaca
     4741 cctgaagaaa tagcagaaca cttggacaaa aaaaatggaa gtgattttct agaaattatg
     4801 aagaatataa aaagctgaat attatctcgt ggtgatacac tgaatttgag gtaaccggct
     4861 catttaaacc gtctggtctg ttacctccgg ttccacaaaa ataatgtcca tcattttgaa
     4921 gtggacacta tcgtatggaa taccggacct ggatcactga agctttacgc cttcatttcg
     4981 aagaacattt accacgggtt gtggccggac gtcgcctggg cgtaccaaaa tcaactgtct
     5041 gtggtatgtt cgtgtgaacc gcccccggta tcccgctcat gctggcgaac ccagacacgc
     5101 agtgaaccgc cccgggtttc ctggagagtg ttttatct
//
LOCUS       DADLEE010000185         5024 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000185 DADLEE010000000
VERSION     DADLEE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.189"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="JQH51_004006"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="JQH51_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY6078135.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="JQH51_004007"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="JQH51_004007"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY6078136.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="JQH51_004008"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="JQH51_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY6078137.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="JQH51_004009"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="JQH51_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY6078138.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="JQH51_004010"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="JQH51_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY6078139.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DADLEE010000186         5014 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000186 DADLEE010000000
VERSION     DADLEE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.190"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..297
                     /locus_tag="JQH51_004011"
     CDS             <1..297
                     /locus_tag="JQH51_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY6078140.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="JQH51_004012"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="JQH51_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY6078141.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="JQH51_004013"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="JQH51_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY6078142.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="JQH51_004014"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="JQH51_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY6078143.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3923)
                     /gene="sitA"
                     /locus_tag="JQH51_004015"
     CDS             complement(3033..3923)
                     /gene="sitA"
                     /locus_tag="JQH51_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY6078144.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="JQH51_004016"
     CDS             complement(4379..>5014)
                     /locus_tag="JQH51_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6078145.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1227 c         1200 g         1264 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat tcggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DADLEE010000187         4973 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000187 DADLEE010000000
VERSION     DADLEE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.191"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JQH51_004017"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JQH51_004017"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY6078146.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JQH51_004018"
     CDS             1020..1703
                     /locus_tag="JQH51_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY6078147.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JQH51_004019"
     CDS             1700..2161
                     /locus_tag="JQH51_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078148.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JQH51_004020"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JQH51_004020"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY6078149.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JQH51_004021"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JQH51_004021"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY6078150.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JQH51_004022"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JQH51_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY6078151.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JQH51_004023"
     CDS             complement(4704..4787)
                     /locus_tag="JQH51_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY6078152.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1239 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DADLEE010000188         4870 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000188 DADLEE010000000
VERSION     DADLEE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.192"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..527
                     /locus_tag="JQH51_004024"
     CDS             <1..527
                     /locus_tag="JQH51_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY6078153.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="JQH51_004025"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="JQH51_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY6078154.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="JQH51_004026"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="JQH51_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="JQH51_004027"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="JQH51_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY6078155.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="JQH51_004028"
     CDS             4169..4342
                     /locus_tag="JQH51_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078156.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="JQH51_004029"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="JQH51_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY6078157.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="JQH51_004030"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="JQH51_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1295 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
     4861 aatactagtt
//
LOCUS       DADLEE010000189         4663 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000189 DADLEE010000000
VERSION     DADLEE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.193"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(337..627)
                     /locus_tag="JQH51_004031"
     CDS             complement(337..627)
                     /locus_tag="JQH51_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078158.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="JQH51_004032"
     CDS             complement(1359..2201)
                     /locus_tag="JQH51_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY6078159.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="JQH51_004033"
     CDS             complement(2286..2483)
                     /locus_tag="JQH51_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY6078160.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="JQH51_004034"
     CDS             complement(2503..2991)
                     /locus_tag="JQH51_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078161.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="JQH51_004035"
     CDS             complement(2988..3365)
                     /locus_tag="JQH51_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY6078162.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="JQH51_004036"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="JQH51_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HAY6078163.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="JQH51_004037"
     CDS             complement(3869..4090)
                     /locus_tag="JQH51_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY6078164.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="JQH51_004038"
     CDS             complement(4177..4653)
                     /locus_tag="JQH51_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY6078165.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1090 a         1116 c         1228 g         1229 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gcc
//
LOCUS       DADLEE010000190         4603 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000190 DADLEE010000000
VERSION     DADLEE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.194"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(79..1002)
                     /gene="phoH"
                     /locus_tag="JQH51_004039"
     CDS             complement(79..1002)
                     /gene="phoH"
                     /locus_tag="JQH51_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY6078166.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="JQH51_004040"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="JQH51_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY6078167.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /locus_tag="JQH51_004041"
     CDS             complement(2745..3872)
                     /locus_tag="JQH51_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY6078168.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /locus_tag="JQH51_004042"
     CDS             complement(3930..>4603)
                     /locus_tag="JQH51_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY6078169.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DADLEE010000191         4552 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000191 DADLEE010000000
VERSION     DADLEE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.195"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..1224
                     /locus_tag="JQH51_004043"
     CDS             <1..1224
                     /locus_tag="JQH51_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY6078170.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="JQH51_004044"
     CDS             1224..1958
                     /locus_tag="JQH51_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6078171.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..3186
                     /locus_tag="JQH51_004045"
     CDS             2425..3186
                     /locus_tag="JQH51_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY6078172.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3230..4309
                     /locus_tag="JQH51_004046"
     CDS             3230..4309
                     /locus_tag="JQH51_004046"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6078173.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4306..>4552
                     /locus_tag="JQH51_004047"
     CDS             4306..>4552
                     /locus_tag="JQH51_004047"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY6078174.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDWICPY"
BASE COUNT         1247 a          867 c         1121 g         1317 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctacacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa
     3181 tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg
     3241 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
     3301 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
     3361 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
     3421 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
     3481 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
     3541 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
     3601 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
     3661 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
     3721 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
     3781 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
     3841 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
     3901 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
     3961 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
     4021 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
     4081 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
     4141 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
     4201 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     4261 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     4321 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     4381 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     4441 ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat
     4501 aaatatcgta ttcgttatag taaatccatt agtgattgga tttgccccta ta
//
LOCUS       DADLEE010000192         4461 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000192 DADLEE010000000
VERSION     DADLEE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.196"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..143
                     /locus_tag="JQH51_004048"
     CDS             <1..143
                     /locus_tag="JQH51_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6078175.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            109..486
                     /locus_tag="JQH51_004049"
     CDS             109..486
                     /locus_tag="JQH51_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY6078176.1"
                     /translation="MLPSETMICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELE
                     AKCAAQENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQ
                     VGDLENVNITHWAVMPKAPEAGA"
     gene            486..791
                     /locus_tag="JQH51_004050"
     CDS             486..791
                     /locus_tag="JQH51_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078177.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            791..1141
                     /locus_tag="JQH51_004051"
     CDS             791..1141
                     /locus_tag="JQH51_004051"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6078178.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            1018..2181
                     /locus_tag="JQH51_004052"
     CDS             1018..2181
                     /locus_tag="JQH51_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6078179.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            2530..3702
                     /locus_tag="JQH51_004053"
     CDS             2530..3702
                     /locus_tag="JQH51_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY6078180.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(3756..4379)
                     /locus_tag="JQH51_004054"
     CDS             complement(3756..4379)
                     /locus_tag="JQH51_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078181.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAEQPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT         1410 a          943 c          954 g         1154 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataga tatgcagcgc cgggtggcat ggataaaccc ggaagagagt
     1741 aaatcaaacc gcgcaattgg cgttgcgctg aatgatactg catgtcgcgt attgaaaaaa
     1801 caaatcggga atcatcaccg ttgggtattt gtgtacaagg aaagctgtac caaaccagac
     1861 ggaacgaaag cgccaacggt caggaagatg cggtatgacg caaacacagc ctggaaagcg
     1921 gcgctgagac gagcaggtat tgatgatttc agatttcacg acttgagaca cacctgggca
     1981 agttggctgg ttcaagccgg agtcccgttg tcagtgttac aggaaatggg aggctgggag
     2041 tctatcgaaa tggttcgtcg atatgctcac cttgcaccta atcaccttac cgaacacgca
     2101 cggcaaatag actcgatcct gaacccatcg gtcccaaatt cgtcccagtc aaaaaataag
     2161 gaaggtacta atgatgtgta acttattgat ttaaatggtg ccgataatag gagtcgaacc
     2221 tacgaccttc gcattatgaa tctgtagcac caatcataac tatctgtttt agcaagcatt
     2281 aaccgcattc actaagctat agttgatgac acaaacagaa agttgatgca tgatgttgcc
     2341 atatatatgt cacaaatacg gcacaacgat cttcaaacat atagccacac attcacagaa
     2401 gagcacaaag ccttgcaatc cagtgcaaag cttggtgtgt ctccgttttg tttcatcagt
     2461 tacacataac tatatgatca ttaactaaaa gtcagtatac ttggagaaat tttaattgcg
     2521 taatagagca tgcatatgat tgaaattaac agtttattat taataacatc cgtgatattg
     2581 atgtcgttat tagctgtggg gttatttgat aaaatttccc caataaatct tgttgagcac
     2641 ggcaggaata atcaaataga tggaatgcgg gggttcttag caattttcgt gcttattcat
     2701 cacgcagcaa tttggaatgg atacttgtca tctggagtat gggaagcacc ttcatcaaat
     2761 ctgttagcaa atttaggcca agttggtgtg tcattctttt ttatgattac tggttatctg
     2821 ttcttttcaa agattatctc tggagatcag gactggacaa ggctttatgt atcaagatta
     2881 ctacgattaa ccccaatgtt catagttagc ctatgtctca tttttatcat tgtaggtttt
     2941 aagtctggat ggagaatgca ggtatccaca gaagagcttt ttgtgtcaat aatgaagtgg
     3001 ttgccattca ctgcgctagg tatgccgaac attaatgacg taaaagattc atttactatc
     3061 aatgccgctg taacatggac acttgtatat gaatggttct tttatttttc tcttccggta
     3121 atttccgcgc tcataaaaag aaaagtcagt atttacatgg ttatgattag cgcaatatcg
     3181 ctatttgttt tcattttatt tttcagtaaa atacacatag catcatttct ttttgggctc
     3241 ctggcatttt tgctaaataa atcaaagata gtcaatggaa ttgccaaagc aaaagtaaca
     3301 ccaattataa taactgcgat aatggttttt gaaatgacgt atttcaaaac aacttacgca
     3361 ccgctaccac tgattctttg tggaataaca tttatcatta tcgcatcagg ttgtgatttg
     3421 tatggaatat taagattaaa tataaccaga aagctaggag aaactaccta cagcgtttac
     3481 cttctgcatg ggatattcct ttattgccta atgacgtgga ttattcctaa taattacact
     3541 gaaaatacat ttatcatact ggtaagcaca actgcatttc ttattacgtt cacatcatgc
     3601 ctaactttta aattaataga gacgccattc atcaaattaa caaaacaaac cacaacttta
     3661 gtaaaagaat taatacccac attaacaaac aacaatcaat aaacaataaa cataagccgc
     3721 cctacatata tgtccaaatg gggcggcgaa tgtatttata ttagtctggt aatgctaaat
     3781 gcaaacgaac tggagattcc tgccccgcta agcgtattgc gcagggtcac cctaaatgta
     3841 tctacgccaa tataagtaac atcacaacta actgaatcag gcgcagatat aggtgttatc
     3901 tgcacaatat aaccgctagc aactgctgcg agcggatgag aaaagctaac atcatacaca
     3961 cctgtagaaa ccacgttaac cgatgaaaca ccagtagcaa cgtcgatact accattggcg
     4021 ttaatttttc ccgagcgtgt gactgacaat gcagaagccg atgttacctt actggttgtt
     4081 tctgtaaaaa cacacccaat gtcaactacc tcgcaattag ctcctgttat ccagtatggt
     4141 tgttctgcac ttggctgata atcccctgcg ctctggaacg ttgtgccgtt gagtatgact
     4201 gtaacttttc ccccacctga gcttgttact tgaatattcg tgtgcgtgta tcttgcactg
     4261 ctcacgcgat gaaaatttcc accgtttata accacagtta cagggcgcgt cccagtatta
     4321 tctatagcaa ggtctgcccc cccggcatta gcctcaaagt atgggttgtt taataccaaa
     4381 gcgcacgaac cgttaaggcc atttatagct aattgcgcac cacctgtact ggaatccccc
     4441 tgagtaccat ttccttcaca a
//
LOCUS       DADLEE010000193         4227 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000193 DADLEE010000000
VERSION     DADLEE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.199"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(34..825)
                     /gene="aadA1"
                     /locus_tag="JQH51_004055"
     CDS             complement(34..825)
                     /gene="aadA1"
                     /locus_tag="JQH51_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY6078182.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(883..1407)
                     /gene="sat2"
                     /locus_tag="JQH51_004056"
     CDS             complement(883..1407)
                     /gene="sat2"
                     /locus_tag="JQH51_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY6078183.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1502..1975)
                     /locus_tag="JQH51_004057"
     CDS             complement(1502..1975)
                     /locus_tag="JQH51_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY6078184.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2307..2843
                     /locus_tag="JQH51_004058"
     CDS             2307..2843
                     /locus_tag="JQH51_004058"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HAY6078185.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2958..3284
                     /locus_tag="JQH51_004059"
     CDS             2958..3284
                     /locus_tag="JQH51_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6078186.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(3472..3711)
                     /locus_tag="JQH51_004060"
     CDS             complement(3472..3711)
                     /locus_tag="JQH51_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY6078187.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1129 a          982 c          925 g         1191 t
ORIGIN      
        1 gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc
       61 gcctttcacg tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
      121 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
      181 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
      241 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
      301 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
      361 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
      421 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
      481 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
      541 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
      601 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
      661 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
      721 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
      781 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
      841 aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc
      901 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
      961 cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg
     1021 tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt
     1081 tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac
     1141 aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag
     1201 ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat
     1261 gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag
     1321 gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac
     1381 ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa
     1441 acctggcgcg gcttttggta caaaaggcgt gacaggtttg cgaatccgtt gctgccactt
     1501 gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag tcttgggtaa
     1561 aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc ggctcgatgt
     1621 ctattgtaga tatatgtagt gtatctactt gatcgatcag gcttttgtat atctccccac
     1681 cacctgaaac aatgacatga tccgttattt tctttaggtt ggttaaagca tctttaattg
     1741 atggaaagat caatacgttc tcattgtcag atgtaaaact tgaacgtgtt acgaccgcat
     1801 actttcggtt gggtaatgct cccattgatt caaaagtctt gcgtccaacc aacagccatt
     1861 ggttataggt aatagcttta aacaggagct gttcaccttt ggcactccat ggaatatcag
     1921 ggccattccc gataactcca ttcttcgata tagctaccat tagtgatagt ttcacaattc
     1981 ttcctcagag gttaacattt tattagtaag catgctcgtt ttgtcacccg ctgatgctta
     2041 ccgttaatta atataatcac ttgtattaaa tagactttct ttaaaaatac aagacactct
     2101 gttattacaa atcgtgcatg ccgtctatcc tgcttgcacg atgcacatta tctcacccaa
     2161 agaactcata acattttaat ttatttagca ttaaattaaa actctaccgg taaacaagca
     2221 tctctaggcg taaaaaacag gcactttttt aaatcccagc tagaataggc tgtataggca
     2281 gacagttgca agacaaggag cgttttatgt ctaacagtcc atttttaaat tctatacgca
     2341 cggatatgcg acaaaaaggt tatgcgctga aaactgaaaa aacttacctg cactggatta
     2401 agcgttttat tctgtttcac aaaaaacgtc atcctcagac catgggcagt gaagaggtca
     2461 ggctgttttt atccagctta gcaaacagca gacatgtagc cataaacacg cagaaaatcg
     2521 ctttaaatgc cctagctttt ttgtacaaca ggtttttaca acagccgttg ggcgatattg
     2581 attatatccc tgcaagcaag cctagacggc taccctctgt tatctctgca aatgaagtgc
     2641 aacgcatttt gcaggttatg gatactcgca accaagttat ttttacgctg ctgtatggtg
     2701 caggtttgcg cattaatgaa tgcttgcgtt tgcgggttaa agattttgat tttgataatg
     2761 gctgcatcac tgtgcatgac ggtaagggtg ggaaaagcag aaacagccta ctgcccacgc
     2821 gcctaatccc agcaataaaa taactcattg agcaagcgcg gcttattcag caagacgaca
     2881 acttacaagg cgtagggcca tcgctgcctt ttgctttaga tcacaaatac ccttctgctt
     2941 atcgacaagc ggcgtggatg tttgtctttc cctccagcac gctctgcaac cacccgtata
     3001 acggcaaatt atgccgccat catctgcatg actccgttgc gcgaaaggca ttgaaggcag
     3061 ccgtacaaaa agcaggcatc gttagcaagc gtgtcacttg tcatacattt cgtcactcgt
     3121 ttgctacgca tctattacaa gcggggcgtg atattcgcac tgtgcaagaa ctcttagggc
     3181 ataacgatgt taagaccacg caaatctata cgcatgtgtt gggtcagcat tttgccggca
     3241 ccaccagtcc tgcggatgga ctgatgctac ttatcaatca gtaaaagtag ataaatatct
     3301 ttatgtagcc ctgagtagcc cctgatactt tttatggtga aaaagataaa caatcataga
     3361 ggggataaat atgagccagt tctaccattg ttcagataag tttaagcaag atactgtcaa
     3421 tcacagtgac cattcatggt cattacactt cctccagttg ccagacaatg cctaacataa
     3481 aaacgcaaaa gcccaaaagt cagagcttag gtgttctagg cgttttctaa gcgctgaaga
     3541 aatacgtctt accgtagatt gcgtgcgttt ctgaagcaca ttttccttat acatggcttt
     3601 atcccaggca gcttcatcaa gctcttggag taacaagcca gtaataatgc gacactctcg
     3661 ggtttgtagc gagccgctgg tgagatcgga gttatagcgg aaaggggtca tttgtaatta
     3721 tttgtgggtg gaggatagtt aacttattat tgtagttgga agaggctgct ttccaaacat
     3781 tagcacctcg taatgcttgt tactgtatat taatttatgc tcttagcgca tgttaatggg
     3841 ctattacaat gcataatgtc cacatcaaga taactgtggc ttaacacctg tacaagcgaa
     3901 aaaaatcaat cacctgtccg gaaatacggg atcactgtaa agtgttggtg aggcagtcta
     3961 ggagggggat ttaatttggg atactgatga aaggattatg atcagttgga tataggatcg
     4021 tactaagctg agcggcaggt tagctagatt agttaaccag ataagtgaaa tctagttcca
     4081 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     4141 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     4201 aactattttg tccgcccaca gctgccg
//
LOCUS       DADLEE010000194         4198 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000194 DADLEE010000000
VERSION     DADLEE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.200"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            84..752
                     /locus_tag="JQH51_004061"
     CDS             84..752
                     /locus_tag="JQH51_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078188.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="JQH51_004062"
     CDS             complement(995..1690)
                     /locus_tag="JQH51_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY6078189.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="JQH51_004063"
     CDS             complement(1683..3110)
                     /locus_tag="JQH51_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY6078190.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="JQH51_004064"
     CDS             complement(3121..3840)
                     /locus_tag="JQH51_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY6078191.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1118 a         1085 c          869 g         1126 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttctggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADLEE010000195         4186 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000195 DADLEE010000000
VERSION     DADLEE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.201"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="JQH51_004065"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="JQH51_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY6078192.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="JQH51_004066"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="JQH51_004066"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY6078193.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1168 a         1021 c          962 g         1035 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttctgg
//
LOCUS       DADLEE010000196         4182 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000196 DADLEE010000000
VERSION     DADLEE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.202"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..186)
                     /locus_tag="JQH51_004067"
     CDS             complement(<1..186)
                     /locus_tag="JQH51_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY6078194.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQH51_004068"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQH51_004068"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY6078195.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="JQH51_004069"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="JQH51_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY6078196.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="JQH51_004070"
     CDS             3863..4144
                     /locus_tag="JQH51_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY6078197.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DADLEE010000197         4168 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000197 DADLEE010000000
VERSION     DADLEE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.203"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..334)
                     /locus_tag="JQH51_004071"
     CDS             complement(<1..334)
                     /locus_tag="JQH51_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY6078198.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..1716)
                     /locus_tag="JQH51_004072"
     CDS             complement(334..1716)
                     /locus_tag="JQH51_004072"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY6078199.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELSAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(1713..2333)
                     /locus_tag="JQH51_004073"
     CDS             complement(1713..2333)
                     /locus_tag="JQH51_004073"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078200.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTMLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(2358..3551)
                     /gene="flhB"
                     /locus_tag="JQH51_004074"
     CDS             complement(2358..3551)
                     /gene="flhB"
                     /locus_tag="JQH51_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY6078201.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            3787..>4168
                     /locus_tag="JQH51_004075"
     CDS             3787..>4168
                     /locus_tag="JQH51_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY6078202.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1022 g          900 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaagacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DADLEE010000198         4114 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000198 DADLEE010000000
VERSION     DADLEE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.204"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..158)
                     /locus_tag="JQH51_004076"
     CDS             complement(<1..158)
                     /locus_tag="JQH51_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY6078203.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JQH51_004077"
     CDS             complement(308..469)
                     /locus_tag="JQH51_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078204.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JQH51_004078"
     CDS             complement(466..669)
                     /locus_tag="JQH51_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY6078205.1"
                     /translation="MLERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JQH51_004079"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JQH51_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY6078206.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JQH51_004080"
                     /pseudo
     CDS             complement(1530..1661)
                     /locus_tag="JQH51_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
     gene            complement(2161..2237)
                     /locus_tag="JQH51_004081"
     tRNA            complement(2161..2237)
                     /locus_tag="JQH51_004081"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JQH51_004082"
     tRNA            complement(2339..2414)
                     /locus_tag="JQH51_004082"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="JQH51_004083"
     CDS             complement(2559..3380)
                     /locus_tag="JQH51_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY6078207.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="JQH51_004084"
     CDS             complement(3395..3751)
                     /locus_tag="JQH51_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY6078208.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="JQH51_004085"
     CDS             complement(3764..>4114)
                     /locus_tag="JQH51_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY6078209.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DADLEE010000199         4042 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000199 DADLEE010000000
VERSION     DADLEE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.205"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(886..1077)
                     /locus_tag="JQH51_004086"
     CDS             complement(886..1077)
                     /locus_tag="JQH51_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY6078210.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="JQH51_004087"
     CDS             1142..1465
                     /locus_tag="JQH51_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY6078211.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="JQH51_004088"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="JQH51_004088"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="JQH51_004089"
     CDS             2143..2673
                     /locus_tag="JQH51_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY6078212.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /gene="mbeD"
                     /locus_tag="JQH51_004090"
     CDS             2680..2913
                     /gene="mbeD"
                     /locus_tag="JQH51_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY6078213.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="JQH51_004091"
     CDS             complement(3459..3851)
                     /locus_tag="JQH51_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078214.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DADLEE010000200         3806 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000200 DADLEE010000000
VERSION     DADLEE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.206"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            437..2089
                     /locus_tag="JQH51_004092"
     CDS             437..2089
                     /locus_tag="JQH51_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY6078215.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="JQH51_004093"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="JQH51_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2349..2462)
                     /locus_tag="JQH51_004094"
                     /pseudo
     CDS             complement(2349..2462)
                     /locus_tag="JQH51_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..2922)
                     /locus_tag="JQH51_004095"
     CDS             complement(2596..2922)
                     /locus_tag="JQH51_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY6078216.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(2987..3778)
                     /gene="ltrA"
                     /locus_tag="JQH51_004096"
                     /pseudo
     CDS             complement(2987..3778)
                     /gene="ltrA"
                     /locus_tag="JQH51_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DADLEE010000201         3738 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000201 DADLEE010000000
VERSION     DADLEE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.207"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            108..2105
                     /locus_tag="JQH51_004097"
     CDS             108..2105
                     /locus_tag="JQH51_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAY6078217.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="JQH51_004098"
     CDS             2168..3445
                     /locus_tag="JQH51_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAY6078218.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DADLEE010000202         3690 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000202 DADLEE010000000
VERSION     DADLEE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.208"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..224
                     /locus_tag="JQH51_004099"
     CDS             <1..224
                     /locus_tag="JQH51_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY6078219.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="JQH51_004100"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="JQH51_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY6078220.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="JQH51_004101"
     CDS             518..1108
                     /locus_tag="JQH51_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY6078221.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="JQH51_004102"
     CDS             1348..1608
                     /locus_tag="JQH51_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY6078222.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /locus_tag="JQH51_004103"
     CDS             1832..1906
                     /locus_tag="JQH51_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY6078223.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="JQH51_004104"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="JQH51_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY6078224.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="JQH51_004105"
     CDS             3457..3648
                     /locus_tag="JQH51_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078225.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          998 a          901 c          929 g          862 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga
//
LOCUS       DADLEE010000203         3550 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000203 DADLEE010000000
VERSION     DADLEE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.209"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..213)
                     /locus_tag="JQH51_004106"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="JQH51_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            512..765
                     /locus_tag="JQH51_004107"
                     /pseudo
     CDS             512..765
                     /locus_tag="JQH51_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            922..2325
                     /locus_tag="JQH51_004108"
     CDS             922..2325
                     /locus_tag="JQH51_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6078226.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFA
                     IIFTLMSRERSLEFEKE"
     gene            2294..2584
                     /locus_tag="JQH51_004109"
     CDS             2294..2584
                     /locus_tag="JQH51_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY6078227.1"
                     /translation="MNAHWSSKKSNFLRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(2678..2869)
                     /locus_tag="JQH51_004110"
                     /pseudo
     CDS             complement(2678..2869)
                     /locus_tag="JQH51_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2910..3407)
                     /locus_tag="JQH51_004111"
                     /pseudo
     CDS             complement(2910..3407)
                     /locus_tag="JQH51_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          865 a          801 c          845 g         1039 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agctgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat
     2221 ctgggatggg gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc
     2281 acactgatga gtcgtgaacg ctcactggag ttcgaaaaag agtaattttc tcaggaaaaa
     2341 cataaaatta ctgactaaat atttattttt tgaatcgcag ggcattccgg ataaggttga
     2401 tatcgtgtca cggctgaaga cctatggata cagtatttct ggtgtggaga cggatgacgg
     2461 atataaggcg cttgtccgcg cttttcagct tcatttccgg cagaaaaatt atgacggcat
     2521 aatggatgct gaaaccgctg ccatcctgta tgcattgctg gagaagtatt tcccgggtaa
     2581 ataaggagtg agtcgtcaga gctgcgatat atctccttcc tccatgggct gtctcttata
     2641 cacaaatccc cacccgggga tttgtgatgt cagatgtccc cagtaagact atgttttcat
     2701 tgcgctctat gaagctgagt gagcgtaacg actggagctg cttctgcggt gctccggtgg
     2761 cgaacgtgaa gtcatactct tcgaacattt tcaccgccgg gaaggctgcc attcgggtat
     2821 acatcgcctg tttacgttga tgacgtgcca gtttttcttc atgaagcatg atgctccaag
     2881 taaacgttca ccgaacaccg tattgacgat tatcctgatg gtagcaccac cttccatggc
     2941 aggaggtcgt caactcggtt ggaaggccat tccggcagta cgctcagaat atggcgcaga
     3001 tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta cagcagtgca
     3061 ccacgctcgc cgccgtgatc gctaccgaag aacacgtaat ttttctttcc gagacagact
     3121 gcacgaagcg ctctttccgc tgtgttattg tccgcctccg ccagaccgtc atcactgtaa
     3181 taacagaggg cgtcccactg attcagtaca tagctgaatg cttcgcccag tctggatttt
     3241 ttcgacagcg tgccattctt ctccaccatc cattcatgca gcgacgtcag taacgctttg
     3301 cttcgctgct gcctgactgc aagacactct gactctggta atccccgtat ttcatcctcg
     3361 atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcacg caagtaatcc
     3421 ggggcccgct atgccgcgct ctatcgggcg ggacggggct ggcgctttga accgccccgg
     3481 gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa
     3541 gcataataag
//
LOCUS       DADLEE010000204         3548 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000204 DADLEE010000000
VERSION     DADLEE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.210"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..465
                     /locus_tag="JQH51_004112"
     CDS             <1..465
                     /locus_tag="JQH51_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6078228.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /locus_tag="JQH51_004113"
     CDS             802..2415
                     /locus_tag="JQH51_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6078229.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQH51_004114"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQH51_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY6078230.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DADLEE010000205         3493 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000205 DADLEE010000000
VERSION     DADLEE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.211"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..311
                     /locus_tag="JQH51_004115"
     CDS             <1..311
                     /locus_tag="JQH51_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6078231.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="JQH51_004116"
     CDS             468..1217
                     /locus_tag="JQH51_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078232.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="JQH51_004117"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="JQH51_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY6078233.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /locus_tag="JQH51_004118"
     CDS             complement(1859..3196)
                     /locus_tag="JQH51_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY6078234.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DADLEE010000206         3488 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000206 DADLEE010000000
VERSION     DADLEE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.212"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..389
                     /locus_tag="JQH51_004119"
     CDS             <1..389
                     /locus_tag="JQH51_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAY6078235.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="JQH51_004120"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="JQH51_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY6078236.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="JQH51_004121"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="JQH51_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY6078237.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="JQH51_004122"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="JQH51_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY6078238.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3071..3481
                     /locus_tag="JQH51_004123"
                     /pseudo
     CDS             3071..3481
                     /locus_tag="JQH51_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DADLEE010000207         3456 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000207 DADLEE010000000
VERSION     DADLEE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.213"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(226..429)
                     /locus_tag="JQH51_004124"
     CDS             complement(226..429)
                     /locus_tag="JQH51_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078239.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="JQH51_004125"
     CDS             complement(431..586)
                     /locus_tag="JQH51_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY6078240.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="JQH51_004126"
     CDS             complement(789..1196)
                     /locus_tag="JQH51_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY6078241.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="JQH51_004127"
     CDS             1273..1500
                     /locus_tag="JQH51_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6078242.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="JQH51_004128"
     CDS             1484..1906
                     /locus_tag="JQH51_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078243.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="JQH51_004129"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="JQH51_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="JQH51_004130"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="JQH51_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          886 a          764 c          939 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacagcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DADLEE010000208         3415 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000208 DADLEE010000000
VERSION     DADLEE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.214"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..238
                     /locus_tag="JQH51_004131"
     CDS             <1..238
                     /locus_tag="JQH51_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY6078244.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="JQH51_004132"
     CDS             248..886
                     /locus_tag="JQH51_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY6078245.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="JQH51_004133"
     CDS             889..2052
                     /locus_tag="JQH51_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY6078246.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3415
                     /locus_tag="JQH51_004134"
     CDS             2136..>3415
                     /locus_tag="JQH51_004134"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY6078247.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGS"
BASE COUNT          760 a          804 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttc
//
LOCUS       DADLEE010000209         3348 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000209 DADLEE010000000
VERSION     DADLEE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.215"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            19..897
                     /locus_tag="JQH51_004135"
     CDS             19..897
                     /locus_tag="JQH51_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY6078248.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="JQH51_004136"
     CDS             894..2285
                     /locus_tag="JQH51_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY6078249.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="JQH51_004137"
     CDS             2282..2539
                     /locus_tag="JQH51_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078250.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="JQH51_004138"
     CDS             2634..2834
                     /locus_tag="JQH51_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY6078251.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="JQH51_004139"
     CDS             2827..3207
                     /locus_tag="JQH51_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY6078252.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DADLEE010000210         3326 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000210 DADLEE010000000
VERSION     DADLEE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.216"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            455..1282
                     /locus_tag="JQH51_004140"
     CDS             455..1282
                     /locus_tag="JQH51_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY6078253.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1326..2075
                     /locus_tag="JQH51_004141"
     CDS             1326..2075
                     /locus_tag="JQH51_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY6078254.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2072..2641
                     /locus_tag="JQH51_004142"
     CDS             2072..2641
                     /locus_tag="JQH51_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078255.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2662..2760
                     /locus_tag="JQH51_004143"
                     /pseudo
     CDS             2662..2760
                     /locus_tag="JQH51_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            2765..3007
                     /locus_tag="JQH51_004144"
     CDS             2765..3007
                     /locus_tag="JQH51_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY6078256.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3166..>3326
                     /locus_tag="JQH51_004145"
     CDS             3166..>3326
                     /locus_tag="JQH51_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY6078257.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          929 a          797 c          854 g          746 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca
      421 tttataaggg tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc
      481 ccttgttctg ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg
      541 catcaaggta aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg
      601 ctactggacg ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac
      661 ctacgatgag ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg
      721 cgtggcctac aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg
      781 cgtggacgac attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact
      841 ggccagcaaa atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc
      901 agctcttaaa gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc
      961 atatgtgggt aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac
     1021 ggcgaaccag aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa
     1081 catgttgcgc gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga
     1141 agccgatgct attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct
     1201 ggaagccatc aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac
     1261 accatttatt caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga
     1321 taaaaatgaa tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc
     1381 agcgtgttgt gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc
     1441 gtgcagcatt caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag
     1501 gtgcggacgt aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg
     1561 ggattgtact ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg
     1621 cctggcaggt tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg
     1681 aaatcgagat gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc
     1741 atgtggaaga acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata
     1801 tgcgcgccgg atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca
     1861 agtgtcggaa tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc
     1921 cggacggtct gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc
     1981 accaaatgat gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc
     2041 ttttccaggt gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc
     2101 cgctctggtg tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac
     2161 gccgaaacat attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg
     2221 gacgaagaaa aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac
     2281 agagctgatt tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact
     2341 gtccattacc ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc
     2401 gaaaaagttt gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga
     2461 gaagatgttt ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg
     2521 gcaagcggtc agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt
     2581 tgttgtgatg ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg
     2641 aaaatcgaat accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta
     2701 actctgggga tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa
     2761 ccacatgttc gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt
     2821 agaaattact cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg
     2881 gcgagtacgc atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca
     2941 caagattctg gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag
     3001 gaagtaaaaa atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta
     3061 cgacctgacg cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga
     3121 ggtctggtgc cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt
     3181 caactggctc ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa
     3241 ccaggcacaa ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac
     3301 gtatcaccag acggtaatcc aaagcc
//
LOCUS       DADLEE010000211         3258 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000211 DADLEE010000000
VERSION     DADLEE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.218"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..442
                     /locus_tag="JQH51_004146"
                     /pseudo
     CDS             1..442
                     /locus_tag="JQH51_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="JQH51_004147"
     CDS             1026..1742
                     /locus_tag="JQH51_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078258.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="JQH51_004148"
     CDS             2294..2698
                     /locus_tag="JQH51_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078259.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="JQH51_004149"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="JQH51_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DADLEE010000212         3189 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000212 DADLEE010000000
VERSION     DADLEE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.219"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="JQH51_004150"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="JQH51_004150"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY6078260.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="JQH51_004151"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="JQH51_004151"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY6078261.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="JQH51_004152"
     tRNA            2652..2727
                     /locus_tag="JQH51_004152"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..>3189)
                     /locus_tag="JQH51_004153"
     CDS             complement(2742..>3189)
                     /locus_tag="JQH51_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAY6078262.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          878 g          752 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct
     2761 tccttatttc ttgactggga caaatttggg accgatgggt tcagaatcaa gtctatttgc
     2821 cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     2881 gactcccagc ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa
     2941 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caataccagc ccgtctcagc
     3001 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc ttactgttgg cgctttcgtt
     3061 ccgtctggtt tggtacagct ttccttgtac acaaataccc aacggtgatg atttccgatt
     3121 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     3181 ttactctct
//
LOCUS       DADLEE010000213         3180 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000213 DADLEE010000000
VERSION     DADLEE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.220"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="JQH51_004154"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="JQH51_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY6078263.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="JQH51_004155"
     CDS             complement(1772..1906)
                     /locus_tag="JQH51_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY6078264.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1931..2365)
                     /locus_tag="JQH51_004156"
                     /pseudo
     CDS             complement(1931..2365)
                     /locus_tag="JQH51_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            complement(2552..2746)
                     /locus_tag="JQH51_004157"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="JQH51_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          775 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg gggggggacc ctgagcaggt ggtaattgta agacgttgcg
     2821 ggcgacggta ttacaattgc acatcctgtc ctgtttcttt aggacgtatc atggcacagc
     2881 atatcaactg ataaataatc cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg
     2941 cgtcgagtgt ttcttaatca ataattccgc tgatactccg gaaaaacgcg agtctgttct
     3001 catgcctgaa atgcccacac cccacgcaaa aacaagtttt tgctgatttt tctttataaa
     3061 tagagagtta tgacaaatta gttcttcttg ctctctttgt gatatttaaa aaagcggtgt
     3121 cggcgcggtg ttgtagctgc gccaacaccg ctttttaggg gtggtactga ctattttcat
//
LOCUS       DADLEE010000214         3112 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000214 DADLEE010000000
VERSION     DADLEE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.221"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="JQH51_004158"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="JQH51_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY6078265.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="JQH51_004159"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="JQH51_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="JQH51_004160"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="JQH51_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY6078266.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="JQH51_004161"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="JQH51_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY6078267.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="JQH51_004162"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="JQH51_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DADLEE010000215         3099 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000215 DADLEE010000000
VERSION     DADLEE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.222"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(106..1815)
                     /locus_tag="JQH51_004163"
     CDS             complement(106..1815)
                     /locus_tag="JQH51_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY6078268.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="JQH51_004164"
     CDS             complement(2247..2966)
                     /locus_tag="JQH51_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY6078269.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DADLEE010000216         3069 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000216 DADLEE010000000
VERSION     DADLEE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.223"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..498
                     /locus_tag="JQH51_004165"
     CDS             <1..498
                     /locus_tag="JQH51_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6078270.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="JQH51_004166"
     CDS             578..1273
                     /locus_tag="JQH51_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY6078271.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="JQH51_004167"
     CDS             1302..>3069
                     /locus_tag="JQH51_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6078272.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DADLEE010000217         3069 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000217 DADLEE010000000
VERSION     DADLEE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.224"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            11..781
                     /gene="lsrF"
                     /locus_tag="JQH51_004168"
     CDS             11..781
                     /gene="lsrF"
                     /locus_tag="JQH51_004168"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY6078273.1"
                     /translation="MQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAPLF
                     EHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCA
                     VAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAA
                     EMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMG
                     RNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="JQH51_004169"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="JQH51_004169"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY6078274.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="JQH51_004170"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="JQH51_004170"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY6078275.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="JQH51_004171"
     CDS             complement(1897..2811)
                     /locus_tag="JQH51_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078276.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          798 a          714 c          816 g          741 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccgg
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc tagaaccacg gcagcctgac ggctgcaaat tgaaaactgc ccctgttcgt
     2941 cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt ttattaagga
     3001 ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct gttggtgctg
     3061 agaagctct
//
LOCUS       DADLEE010000218         3065 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000218 DADLEE010000000
VERSION     DADLEE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.225"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..197
                     /locus_tag="JQH51_004172"
     CDS             <1..197
                     /locus_tag="JQH51_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAY6078277.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="JQH51_004173"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="JQH51_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HAY6078278.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="JQH51_004174"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="JQH51_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAY6078279.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="JQH51_004175"
     CDS             1821..2576
                     /locus_tag="JQH51_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAY6078280.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2977..>3065
                     /locus_tag="JQH51_004176"
     CDS             2977..>3065
                     /locus_tag="JQH51_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY6078281.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVF"
BASE COUNT          798 a          683 c          726 g          858 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcag gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggccc
     2761 ctggcgcagc aaagtcaggc atttatactc tttttaacac atagaaaatt catatcaata
     2821 taataaaaac ataaaatatt tgcatataca tataatctta aagtttagtc tatttaatgt
     2881 tcaatgaaat atttctacct gtataatctt taaagatgtt gaacatatat tcacattaaa
     2941 tatgattatg tacttgttac aaggataagg ttataaatga ataaagttac aaaaacagct
     3001 attgtgggct tacttgcgct tttcgcaggt aatgccgctg caaccgatgg tgaaatagtt
     3061 tttga
//
LOCUS       DADLEE010000219         2951 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000219 DADLEE010000000
VERSION     DADLEE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.226"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JQH51_004177"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JQH51_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY6078282.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH51_004178"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JQH51_004178"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY6078283.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="JQH51_004179"
     CDS             2603..>2951
                     /locus_tag="JQH51_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY6078284.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DADLEE010000220         2932 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000220 DADLEE010000000
VERSION     DADLEE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.227"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="JQH51_004180"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="JQH51_004180"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY6078285.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /locus_tag="JQH51_004181"
     CDS             643..1602
                     /locus_tag="JQH51_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6078286.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="JQH51_004182"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="JQH51_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY6078287.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2932
                     /locus_tag="JQH51_004183"
     CDS             2753..>2932
                     /locus_tag="JQH51_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HAY6078288.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DADLEE010000221         2894 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000221 DADLEE010000000
VERSION     DADLEE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.228"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..308)
                     /locus_tag="JQH51_004184"
     CDS             complement(<1..308)
                     /locus_tag="JQH51_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY6078289.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="yehT"
                     /locus_tag="JQH51_004185"
     CDS             complement(355..1074)
                     /gene="yehT"
                     /locus_tag="JQH51_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY6078290.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="JQH51_004186"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="JQH51_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY6078291.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DADLEE010000222         2714 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000222 DADLEE010000000
VERSION     DADLEE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.229"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            6..224
                     /locus_tag="JQH51_004187"
     CDS             6..224
                     /locus_tag="JQH51_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY6078292.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="JQH51_004188"
     CDS             226..591
                     /locus_tag="JQH51_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY6078293.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="JQH51_004189"
     CDS             588..1253
                     /locus_tag="JQH51_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078294.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="JQH51_004190"
     CDS             1253..1618
                     /locus_tag="JQH51_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY6078295.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2016..2228
                     /locus_tag="JQH51_004191"
     CDS             2016..2228
                     /locus_tag="JQH51_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6078296.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            2465..>2714
                     /locus_tag="JQH51_004192"
     CDS             2465..>2714
                     /locus_tag="JQH51_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078297.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          771 a          557 c          696 g          690 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtt
//
LOCUS       DADLEE010000223         2711 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000223 DADLEE010000000
VERSION     DADLEE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.230"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            50..2572
                     /locus_tag="JQH51_004193"
     CDS             50..2572
                     /locus_tag="JQH51_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY6078298.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="JQH51_004194"
     CDS             2583..>2711
                     /locus_tag="JQH51_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6078299.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DADLEE010000224         2629 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000224 DADLEE010000000
VERSION     DADLEE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.231"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..546)
                     /locus_tag="JQH51_004195"
     CDS             complement(<1..546)
                     /locus_tag="JQH51_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078300.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2197)
                     /locus_tag="JQH51_004196"
     CDS             complement(1208..2197)
                     /locus_tag="JQH51_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078301.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2281..>2629)
                     /locus_tag="JQH51_004197"
     CDS             complement(2281..>2629)
                     /locus_tag="JQH51_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY6078302.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          683 a          613 c          632 g          701 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gagttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa gacaggtgaa
      841 tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac ttgaagcacg
      901 taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt ttggtctggc
      961 tcaggtcagg ctatgggggc ttttggctcg ctctttatat tagatgaggt ctcacatttt
     1021 tcagtcaaaa ctcatcgtct aatagcccat agccagacca ccagtcccag ctttagcagg
     1081 aagaccacga gatagacttt gtgtaggtcc atttggccct cctactttct gtattcacaa
     1141 actagattac tagtacaaaa taaaggccag acatccagtt cggaagtctg accaatagtc
     1201 gttattgtta gttagcttct ttagtgaagc tcaggcgttt gactacaggc aggacagcag
     1261 tagccgagcc agtacccatc ttgatggtcg tagcagcttc agctacaaca aacaccttgc
     1321 cgagaatctc aaggtcgtga gactgggagt ctccatcacg tgaaaggacc acagtaatgc
     1381 tctcgccatc gtgacggaag caaccgaagc tcagagccgt aaaacgctta gccatcatga
     1441 acacgccacg gtcttcaccg ttgtaatagg cgacgtcagt ccccagcata tcgacgatag
     1501 cgtcttcacc accgaagccc tgcttagcag ccagacggga gagagccttg aggtgtgact
     1561 catgggcgat aatgccgtag tcagaaacct cagaaccgaa agaagactcc gtgttctcgt
     1621 acagggcatc catgatagcg tcagccttag cggtcaggct accagtcagg gccggaagtt
     1681 ctacggtctt cagcagcttt gaggactcca gcacgtcaac catcttagag ttaatggtac
     1741 gaatcacgtt atcgcagatg ttcagtgcgt cctgctggag cgtcccctga gtgaaaccag
     1801 tggtcgactt ctggtcccaa agctttgttt ggtgagacac gaggatcgcc tgttcaatcg
     1861 gctcaacggt gatttcagtg ccagacggag cggtcacaac ttcaggtcgc ttaccaccac
     1921 ggacagggac cagcacagag cggtcagcca gagtgtgagc aatgattgga gtcggtaagc
     1981 tgttacgggt cggcaggact tctacaggga acgggatgtt atcttgagcg tctttaagta
     2041 caccgttcag catagcctta acagtcttag ctaactggag ggcatagacc tgagggtcgg
     2101 cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct
     2161 caatgccaaa ctggaaagcg tttttagtca tattcattat aattaatctc cttgagtcaa
     2221 atacttgaag tccctaacag gcagtcaggg accgtgtgtt ctcagatgag ggacgcttgc
     2281 ttacaagctg tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacgtga
     2341 gcgaccagcg gaatgagtag cgctgcggag gttttcacca taggtccggc gttcagcttt
     2401 gacctgttcg cggcgctgta gctctttttg ccattcgtct tcggtcatga acatttcgcg
     2461 ggtacgctct ttctgagacg ctagctcttt agcgatacgc tcagcttcag ctcgtttgtt
     2521 catctggcgg cgttggtagg cggtcatcag gtcattactg aagaagttac caatggtcac
     2581 gccagcttca gccagagctt ccagtaactc catcgcctcg cggctggtg
//
LOCUS       DADLEE010000225         2572 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000225 DADLEE010000000
VERSION     DADLEE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.232"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(64..822)
                     /locus_tag="JQH51_004198"
     CDS             complement(64..822)
                     /locus_tag="JQH51_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY6078303.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JQH51_004199"
     CDS             complement(830..1933)
                     /locus_tag="JQH51_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY6078304.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..2554)
                     /locus_tag="JQH51_004200"
     CDS             complement(1943..2554)
                     /locus_tag="JQH51_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY6078305.1"
                     /translation="MAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLPLLAQWL
                     FGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQH
                     EAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSLFAG
                     TVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          711 a          683 c          601 g          577 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DADLEE010000226         2517 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000226 DADLEE010000000
VERSION     DADLEE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.233"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..440
                     /gene="capU"
                     /locus_tag="JQH51_004201"
     CDS             <1..440
                     /gene="capU"
                     /locus_tag="JQH51_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY6078306.1"
                     /translation="CWLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRV
                     ASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMC
                     ALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
     gene            445..1395
                     /gene="virK"
                     /locus_tag="JQH51_004202"
     CDS             445..1395
                     /gene="virK"
                     /locus_tag="JQH51_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY6078307.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1460..2404
                     /gene="lpxM"
                     /locus_tag="JQH51_004203"
                     /gene_synonym="msbB"
     CDS             1460..2404
                     /gene="lpxM"
                     /locus_tag="JQH51_004203"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY6078308.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          723 a          483 c          574 g          737 t
ORIGIN      
        1 tctgttggtt gattgtgggg tctggttcac ctgaactgcg ggaacattta cagtatcaga
       61 ttgacagtat gggcatgcat gatgatgttt ttattgctga caatgttttt cctgccgccc
      121 ccgtatatcg ggttgccagt ctggtggttc tgccttcaga aaacgaatct tttggtatgg
      181 tgctggcaga agcatcggca ttttctgtgc ctgtagtggc cactcagatt ggtggaatcc
      241 ctgaggttat tcagaacaac cagaccggga cattgttacc agcaagtaat aagcacgcat
      301 ggatgtgcgc cctgaatgat ttttttaatg accctgggcg tttttatcag atggctcgcc
      361 tggcaaaaca ggatatagaa gagcggtttg atattaataa aactgcgtta aaaatactca
      421 cattagcgaa gcaaaagtaa caatatgttt tctataagta acttatcatt tatcggtttc
      481 cttaaaagga ttattttttc ctcagattca ctaccgggga agtgggaaca cagaaaattt
      541 cggttcatgt acattttgcg atgttctata aatccggttg tcagtattcg atattattat
      601 gaactgcgtt ccttgccgtg cattgaggat attctggcaa tacaccccac gttgccagca
      661 agaattcatc gcccttattt gcataaaggg gggcgcgcct ggacccgtgg acaatatatt
      721 cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat attctaaatt tttgtttcca
      781 caaaaatcgg tatcattagt ccaattcatc gggaaagatg gagagaattt tgatatccag
      841 tgttctccca gtggttttga ccgggaagga gagttgatgt tgtcattgtt ttacaataaa
      901 acagttattg cccggttaac attttcagtc attctgactc aaaatggaca cattgctttt
      961 attggcggat tacagggagc ccctaaaaat accggacctg atgttattcg gtgcgcaacc
     1021 cgggcctgtt atgggatttt cccaaaacgg attatttttg aagcattttg tgcgttgatg
     1081 aaagcttgca atatctctga gtgtctggca gtcagtgagc acagtcatgt tttccggcaa
     1141 ttgagatact ggtatcagaa acgcaaaacc tttgttgcgg tctatagtga tttctgggag
     1201 tctgtagcag gaaaaacctg tggtgactgg tacagattac caacccaggt gattcgaaag
     1261 ccgctaagcg atatagccag taaaaaacgc tctgggtacc gaaaacgata cgctttgctg
     1321 gattatatcc atgaaactac tatccgctct ttggatgcat atcctgtaca ctcagaacat
     1381 cagaatttaa attaaggttt gatgtattct ctgaataaaa cagtaatgat aattttggta
     1441 gaggcattcg cactaaataa tgaaaaaata taaatctgag tttattcctg aatttaagaa
     1501 aaattacctt tcccctgttt actggtctac atggttcctt ttgggaatga ttgcaggtat
     1561 ttcaatgttt cccccttcat tcagagatcc tgtcttggca aaaatagggc gttgggcggg
     1621 cagattgagc aaaaaagctc gtcgcagggc gacgattaat ttatcgcttt gtttcccaga
     1681 aaagagtgat acagaacggg aaataattgt cgacaaaatg tttgtcacag cattgcaatc
     1741 catagtgatg atggcagaac tggcaattcg tggtccggaa aagttccaga aacgagtgtt
     1801 ctggaaaggc ctcgaaattc ttgaggaaat tcgacacaat aatagaaatg tgatttttct
     1861 ggttccccat ggctggagcg tggatattcc tgcaatgttg ctggcagctc agggaaaaaa
     1921 aatggccgcc atgtttcatc agcaacgaaa tccagtgatt gattatgtct ggaattcagt
     1981 acggcgtaaa ttcggggggc gcttacatgc ccgggaggat gggataaaac catttattca
     2041 gtcagtacgc cagggatact ggggatatta cctcccagat caggatcatg gtcctgaata
     2101 cagtgaattt gctgattttt ttgcgaccta taaagcgaca ttaccaatca ttggacgtct
     2161 gatgaacatc agtcaggcta tgattatacc acttttcccg gtttatgatg aaaaaaaaca
     2221 tctactgact attgaaattc ggccaccaat ggatgcttgc attgccagcg cggacaataa
     2281 aacgattgcc cgacaaatga acaaaacagt ggaaattttg gtggggccac atccggaaca
     2341 gtatgtctgg gttttaaaat tgttaaaaac acgcaaatca aacgaagcgg acccgtaccc
     2401 ttgaaattat aaagtacaag tatttccact agttgtttgt aagcgtacag cctgaaccgt
     2461 ctggtcagaa tctgacgaat tagacaaagt ggtgtccacc aaataagtag tgggaac
//
LOCUS       DADLEE010000227         2496 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000227 DADLEE010000000
VERSION     DADLEE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.234"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            52..1038
                     /locus_tag="JQH51_004204"
     CDS             52..1038
                     /locus_tag="JQH51_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY6078309.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVS
                     IAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            1039..1404
                     /locus_tag="JQH51_004205"
     CDS             1039..1404
                     /locus_tag="JQH51_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY6078310.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            1401..2084
                     /locus_tag="JQH51_004206"
     CDS             1401..2084
                     /locus_tag="JQH51_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY6078311.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            2283..2358
                     /locus_tag="JQH51_004207"
     tRNA            2283..2358
                     /locus_tag="JQH51_004207"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Ile,seq:cat)
     gene            2360..2436
                     /locus_tag="JQH51_004208"
     tRNA            2360..2436
                     /locus_tag="JQH51_004208"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Lys,seq:ttt)
BASE COUNT          654 a          572 c          693 g          577 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcctcac
      661 gcccaccagc aacagaatcc accacaggaa gagacggtgg tcagcattgc cgttgatccg
      721 gaatctccgg ctcagtatct ccagcgccag aaaccacaac gggaagagat gcctgtatac
      781 acgcgctggg taaaaacgca gaaatgcatg acgtgtggca atcaggcaga tgatccgcac
      841 cacatcattg gtcatggact gggagggatg ggaacaaagg ctgacgattt gtttgttatt
      901 ccgctgtgtc gtaaatgcca taacgaacta cacgccgggg taaaagattt tgaagaaaaa
      961 cacggtagtc agttgttgtt gctgattcgt tttttaatgc acgcgagaaa ttcgggtgtt
     1021 ctgaagtgga aagcataaat gaccgaacgc atagaatttg ttttgcctta cccgccaacg
     1081 gtgaacacct actggcgacg tcgtggcagc acatattttg tatcaaaagc cggtgagcgt
     1141 tatcgccgtg atgtggcgct aattgttcgc cagcagcgac tgaaattaaa cctgtccgga
     1201 aggctggcga taaagattat tgcagagcca ccggataagc gccgtcgtga cctggacaat
     1261 atcctgaagg caccgctgga tgcgctgacg catgctggac tacttataga cgacgagcag
     1321 tttgatgaaa tcaatattgt gcgcggtcag ctcgttcctg gtgggcggct ggggataaaa
     1381 atcacagaac tggggtgcgc atgaataacc agtatttaca gtttgtgcgt gagcagctca
     1441 ttatcgccac cgctgatttg agtggggcaa caaaaggtca gcttgaagcc tggcaagaga
     1501 atgccatgtt cgatacaggg cgttacaggc gtaaaaaaat ccggtaccgc gatgaagtga
     1561 ctggaaaaat gataacgcgg gataatccac caatcccggg aaagcaatcg ctggcgaagg
     1621 ggacgtcaat tcctctggtc agtccggttg agttttcgac atcatcgtgg cggcgggctg
     1681 ttctgtctct tgaagagcac tataaagcct ggttgttgtg gtgttacagc gggagtattt
     1741 gttgggaata tcagatcgcg ataacacagt gggcgtggaa tgaatttaat gctcaatccg
     1801 gtaccagaaa aattgcaggg aaaacgcagg aacgcctgaa aaaattaatc tggctggcgg
     1861 cgcaggcagt aaaagcagaa ctttttggtg gggaaggtta tgaataccag gagctggcat
     1921 tactggcggg agtgacaact aaaaactggt ccaaaacatt tactcgtcac tgggttgcaa
     1981 tgaaacacat ttttcaccga ctggatagtg aggctttatt gtttgtaatg agaacacgtt
     2041 caaaaaaaag acggcatttt caaagcaaag tgttgcaaaa gtagattgaa aggcatatat
     2101 tcatgcaaat ctgatacttt gccgattttg tacgtgatgg caaaagcaaa caaaacccgc
     2161 ccacaagcgg gttttttgtg ccacttatct cggatagaca tggtgaatgc gctggtggag
     2221 gagataaggg tgatttttga atgcttgcaa cattgatttc gtaacgttat tatcctgcgc
     2281 ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggtc gctggttcaa
     2341 atccagcaag ggccaccaac cgccactagc tcatcaggaa agagcgtcaa ccctttaagt
     2401 tgagtgtgcg aggttcgagt ccccggtggc ggtccagtgc cgacttggct cagtaggtag
     2461 agcaactgac ttgtaatcag taggtcacca gttcga
//
LOCUS       DADLEE010000228         2487 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000228 DADLEE010000000
VERSION     DADLEE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.235"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>2487
                     /locus_tag="JQH51_004209"
     CDS             <1..>2487
                     /locus_tag="JQH51_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY6078312.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DADLEE010000229         2463 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000229 DADLEE010000000
VERSION     DADLEE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.236"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(746..2350)
                     /locus_tag="JQH51_004210"
     CDS             complement(746..2350)
                     /locus_tag="JQH51_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY6078313.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          648 a          575 c          526 g          714 t
ORIGIN      
        1 aagacctata ggtttggtga ttgattatta taaatatcat caccccttaa ggtattccct
       61 tagagttaat ctttaattgg tctttaatta aatcttttat ttaatactta aagttaatct
      121 ctaattgtgt ctttaagtta actttaagta atactataag ttaatcttta attgtgtctt
      181 taagttaact ttaagttaag cccctgtagg gactagagac cataattaag atgattgtgt
      241 cttattgcta ttcacttatc gactgaccca gagcacacag gaagcattca ctttaagtag
      301 aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag gatgcgataa gttacggtta
      361 gttgtcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      421 tccttgacaa gtagagaaaa ccatgagtca aaaacatgac cacacaggtc tgtagtaagt
      481 gtcatcctag aagagaaaac tttttgtgtc ccttctccct atagtgagtc gtattaatta
      541 ccccttgaca agcagagaaa actatgagtc aaaaacatga ccgcacaggt ctgtagtaag
      601 tgtcatccta gaagagaaaa ctttttgtgt cccttctccc tatagtgagt cgtattaatt
      661 atcccttgac aagtagagaa aactatgagt caaaaacatg accacacagg tctgtagtaa
      721 gtgtcatcct agactatggt ctcctctagg tctccttata aacatcagcc atcacattga
      781 ccagcctatc aacctcgacc tctttagcat aacggtcgta cgtaatagac cctgaactat
      841 gccccatgat cgcctgtgca tacgccaact cgaccccatg ttgttttaac tgggccgcaa
      901 ctgtatgtct aaacgagtga aacgctaagc cgctacggtc tacgaggact ttaggcaaca
      961 acgtccgatt aaaccattta ctcacctgct caccgtaacc gttcttcatg agacgcagac
     1021 catcgaacac catagcgtca tcaccgtcag cgcctctacg ggtctctaca agcgccataa
     1081 agtcagccaa tataaaacca taggccccat cgaccagagg cacacagcgg tcactatgag
     1141 cgttcttgat gctcttaccg ggtagactac tatcgtcttc attgatatga atataaacgg
     1201 ttcccgcctc agtggtcctg acgtctttga cctgtagttg ggctatttca ttgagtctcg
     1261 ccccagtgat agcagccaag gcggtaacat aataatgata cggcttacca gaggtcttct
     1321 gagagtatgc cttagctgcg accagtagtt gccccacctg ctccgtagag aaggcgttac
     1381 gggcccctga ggctttacgc tgagggactt taagctccag accctcagtc atgttctttt
     1441 taatcaagtc attgcgcaca gcccacctaa agacagcagc catcttaata agatacttgt
     1501 tgttgatggt aacgacatcc agacaatctg tcttatcttc acggctcaac aggtcagcaa
     1561 gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa ctgctggagc acatcacgga
     1621 ccctaagcat gtcagccctg ttagcgtctg cattgagacc cagatggtcg aatatttcga
     1681 ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc aggcttccag ttctgatagt
     1741 tctccgcttc gtattgttca aagaggctcg ctatggtaat gctaggcgta acttctggtt
     1801 ttacttctgg agcattaaca gatagggaca cagagtctgc ttggttatct tgcctatcaa
     1861 tatcattaaa aagatcgacg taatctatca gaggctgact gtctcccgct tcgatacgcc
     1921 tcatacaggc ttttagcacc ttcagggcct cattgatgta acgatgctgg tcaatgctta
     1981 gcggctcact agccagagca tcggtaaggc tctcaccaag ttctccgtat tggtcccggt
     2041 agatatccct catgtcaggc tcaaagagat ctgaacggcc catagacagt tcacactcag
     2101 cgatgtcctt aaggtgttcc ctcatttcct cataggtagc gttgggtctg tctgcgtgta
     2161 ttgccttgag tgctgccttt atgtgccttg agtaagccat agcggtgcgt ctatccttgg
     2221 tccgtaatga tattgaggtc attctatcat tgtttgattg tctgacccta agacggaagt
     2281 agtagacgcc gttacgctgg tagagatagt agactgctgg aagtttgtga ctactggata
     2341 gctgaatcat atgtctgcat gccctgagag ggttacacag tgggttacag taagtaactc
     2401 tgcaaggagg cttaaagccg tatcccttag gattcagtag gttggagtac tttaagaaag
     2461 gtc
//
LOCUS       DADLEE010000230         2447 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000230 DADLEE010000000
VERSION     DADLEE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.237"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..226
                     /locus_tag="JQH51_004211"
     CDS             <1..226
                     /locus_tag="JQH51_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6078314.1"
                     /translation="ENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPE
                     DWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(480..644)
                     /locus_tag="JQH51_004212"
     CDS             complement(480..644)
                     /locus_tag="JQH51_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078315.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1546..1830
                     /locus_tag="JQH51_004213"
     CDS             1546..1830
                     /locus_tag="JQH51_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY6078316.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1818..2303
                     /locus_tag="JQH51_004214"
     CDS             1818..2303
                     /locus_tag="JQH51_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6078317.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          634 a          521 c          693 g          599 t
ORIGIN      
        1 tgaaaacgcg ttacgatcgg tggcggttgg aagaaaaaat tatctctttt tcggctcaga
       61 caagggagga gaaagtgcgg cgatcatcta cagtctgctg gtcacctgca aacagaacga
      121 agtggagccg gaggactggt tgcgcgaagt gatcgagaag ctcaatgact ggccgtcgaa
      181 ccaagtgcat gaactgctgc cctggaactt ctcgtctgta aaataatcct tacgctacgt
      241 acttctcggg gcgcttacct tcaaaatgat ggacattatt tttgtggaac cggaggtaac
      301 agaccagacg gtttaaatga gccggttact ttaaacgggt gatggacagc acctcaaaag
      361 ttcagatgga ttatatttgt ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg
      421 aacgacttgc tcaggggata gctgatggag ttactgaagt accgaaggac cactaaaaat
      481 cacaggccgg gaatttcggc gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt
      541 gatgttggat gcaatgctgt atgagagatg aggggcggcg tccagacgca gccttcaaac
      601 cgccttgagc tgctgccgtt aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga
      661 acatgtgtca ggtaggtgtt gcggagatga acgaaagtga actgtcgatg aagtgtcgaa
      721 aggaacctct aggcgatgtc gaaaatgtgc tatctacgca gcgcatgacg agtccagcag
      781 atatctggta ctggctgggc ggcatccggc atataggcag catgaacgca acacaggctc
      841 tcgtacggaa cgtgggaacc tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca
      901 ctgcaagcgc ctgagtaccg atgcgagaca caggggcaga tggcttcgta gtagtgagga
      961 agtgcctgta atgggcatgg agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa
     1021 ccggatagcc gggaggagcc catgagtcag ccaaagcagt tcgaggtctc taagtatgcg
     1081 gtttggaaag cataccagcg ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag
     1141 tcagtcgagg cgtttgaggt taccaatcga agctataccc ggtaatggcg caggttgacc
     1201 tgcacttggc caagtggatt gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg
     1261 cattcgagtg gttactgcga attcgtcacg actgcccgac gctgtttgca cactggtgct
     1321 tagcttacga tacttgaatg gttcaacgac aagagccgta tgacgcgaga gtgtcacgta
     1381 cggctccgtg ggagcgtagg ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg
     1441 cggttacgtc cacagaggta aatgccgctg acgcggcatc tgtaatctgt tactttgtgt
     1501 atatcatttg ctatatattg tcaatatgct attgttgtaa agcaaatgat atacggaggc
     1561 tttatgaaat caggtgttca gctcaatctc agagcaagag aatcacagcg gatactcatt
     1621 gatgctgctg cagaaattct tcataagtcg cgtacagatt tcattctgga aatggcctgt
     1681 aaagctgctg aagatgttat tctcgaccgg cgggtgttta attttaatga caggcaatat
     1741 gaagagttca ttgaaatgct tgatgctccg gttgcagatg atcctgccat cgaaaaattg
     1801 ctggcaagga aacctcaatg ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc
     1861 aggtcgctga atttgtcagc agtgaagctg tacttgatga ctggttaaag cagaaggagc
     1921 taaaaaatca ggcaatcgga gccacccgga cttttgttgt atgcagaaaa ggtacgcaac
     1981 aaatagtcgg tttttactca ctggccactg gtagtgtcaa tcatacagaa gctacaggta
     2041 accttcggcg caatatgcca gaccccattc ctgtaattat acttgcacgc ctggctgtag
     2101 atgtgtcatt tcgaggaaaa gggcttggcg cggatttact tcatgatgca gttcgtcgtt
     2161 gttacagggt tgccgaaaat atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa
     2221 acgcaaaaca gttttatatt catcatggtt ttaagccatc aaaaactcag gtgcaaacat
     2281 tgtttcttaa actccctcaa tagggagtaa tgccgatcag ttaaggatca gttgaccgat
     2341 ccagtggctg tgtaagaatc cggaaacgct cactcgtttc cggatttttc tgtgcacagt
     2401 aaaagaccac ctcctgagga ggtggctttt cagaatgccc cctataa
//
LOCUS       DADLEE010000231         2418 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000231 DADLEE010000000
VERSION     DADLEE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.238"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..243
                     /locus_tag="JQH51_004215"
     CDS             <1..243
                     /locus_tag="JQH51_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY6078318.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="JQH51_004216"
     CDS             complement(332..844)
                     /locus_tag="JQH51_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY6078319.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..1803
                     /locus_tag="JQH51_004217"
     CDS             1039..1803
                     /locus_tag="JQH51_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078320.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1754..>2418
                     /locus_tag="JQH51_004218"
     CDS             1754..>2418
                     /locus_tag="JQH51_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY6078321.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          680 a          540 c          614 g          584 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggac
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga
     1741 gagcgtgacc tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg
     1801 tagtttctgg actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa
     1861 atcatgggat gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg
     1921 ttgtgcccgt cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca
     1981 gatagacagg tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg
     2041 ggttactggc tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc
     2101 actggagggg atcgatatgt tgtggttgga agaagcccac gcactgacgg aataccagtg
     2161 gaaaattctg gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc
     2221 cggacttgtt actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct
     2281 catccgcaaa attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat
     2341 cgacgcggcg cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga
     2401 gtctgatgat gatgcggc
//
LOCUS       DADLEE010000232         2371 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000232 DADLEE010000000
VERSION     DADLEE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.239"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..823)
                     /locus_tag="JQH51_004219"
     CDS             complement(<1..823)
                     /locus_tag="JQH51_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6078322.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="JQH51_004220"
     CDS             complement(1422..1670)
                     /locus_tag="JQH51_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY6078323.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2025..>2371)
                     /locus_tag="JQH51_004221"
     CDS             complement(2025..>2371)
                     /locus_tag="JQH51_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY6078324.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          574 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DADLEE010000233         2325 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000233 DADLEE010000000
VERSION     DADLEE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.240"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            61..282
                     /gene="kil"
                     /locus_tag="JQH51_004222"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="JQH51_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY6078325.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="JQH51_004223"
     CDS             276..452
                     /locus_tag="JQH51_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078326.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="JQH51_004224"
     CDS             527..802
                     /locus_tag="JQH51_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078327.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="JQH51_004225"
     CDS             902..>2325
                     /locus_tag="JQH51_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY6078328.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DADLEE010000234         2250 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000234 DADLEE010000000
VERSION     DADLEE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.241"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JQH51_004226"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JQH51_004226"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY6078329.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DADLEE010000235         2218 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000235 DADLEE010000000
VERSION     DADLEE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.242"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQH51_004227"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQH51_004227"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY6078330.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQH51_004228"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQH51_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY6078331.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KYPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2210)
                     /gene="arsR"
                     /locus_tag="JQH51_004229"
     CDS             complement(1881..2210)
                     /gene="arsR"
                     /locus_tag="JQH51_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY6078332.1"
                     /translation="MFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRHLA
                     LLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNCSG
                     DSKNICS"
BASE COUNT          590 a          610 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggata tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DADLEE010000236         2208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000236 DADLEE010000000
VERSION     DADLEE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.243"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..124
                     /locus_tag="JQH51_004230"
     CDS             <1..124
                     /locus_tag="JQH51_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078333.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="JQH51_004231"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="JQH51_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY6078334.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            2131..>2208
                     /locus_tag="JQH51_004232"
     CDS             2131..>2208
                     /locus_tag="JQH51_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY6078335.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          679 a          400 c          376 g          753 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ctaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgat atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaagag cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga
     2101 tggtgatttt aaggtgcttg cgtagcttcc atttccatca gatgtccttc ctgctccgct
     2161 actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgc
//
LOCUS       DADLEE010000237         2183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000237 DADLEE010000000
VERSION     DADLEE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.244"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            12..203
                     /locus_tag="JQH51_004233"
     CDS             12..203
                     /locus_tag="JQH51_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAY6078336.1"
                     /translation="MPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVENVVD
                     VYVTHNPKKTEQKAGVSIH"
     gene            227..694
                     /locus_tag="JQH51_004234"
     CDS             227..694
                     /locus_tag="JQH51_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078337.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGR
                     YYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="JQH51_004235"
     CDS             696..1301
                     /locus_tag="JQH51_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY6078338.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="JQH51_004236"
     CDS             1288..>2183
                     /locus_tag="JQH51_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078339.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DADLEE010000238         2165 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000238 DADLEE010000000
VERSION     DADLEE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.245"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            156..341
                     /locus_tag="JQH51_004237"
                     /pseudo
     CDS             156..341
                     /locus_tag="JQH51_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(441..731)
                     /locus_tag="JQH51_004238"
     CDS             complement(441..731)
                     /locus_tag="JQH51_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY6078340.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(700..951)
                     /locus_tag="JQH51_004239"
     CDS             complement(700..951)
                     /locus_tag="JQH51_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6078341.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLRWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            1403..1687
                     /locus_tag="JQH51_004240"
     CDS             1403..1687
                     /locus_tag="JQH51_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY6078342.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1687..1962
                     /locus_tag="JQH51_004241"
     CDS             1687..1962
                     /locus_tag="JQH51_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY6078343.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            2015..>2165
                     /locus_tag="JQH51_004242"
     CDS             2015..>2165
                     /locus_tag="JQH51_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078344.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQ"
BASE COUNT          601 a          536 c          521 g          507 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg ggatatatcg
      421 cagctctgac gactcactcc ttatttaccc gggaaatact tctccagcaa tgcatacagg
      481 atggcagcgg tttcagcatc cattatgccg tcataatttt tctgccggaa atgaagctga
      541 aaagcgcgga caagcgcctt atatccgtca tccgtctcca caccagaaat actgtatcca
      601 taggtcttca gccgtgacac gatatcaacc ttatccggaa tgccctgcga ttcaaaaaat
      661 aaatatttag tcagtaattt tatgtttttc ctgaaaaaat tactcttttt cgaactccag
      721 tgagcgttca cgactcatca gtgtgaaaat gatggcaaaa acagcagaca gtatcagtac
      781 gataaatgtg gccccccatc tcagataatc gacaacaagt cccattccca gtgtcgcaac
      841 aattgagccg aagatatagg aaaatgtgcc tgacagccct aacagaataa gtttacgcgt
      901 aaatcgccct gaatccagaa gagcattaag tatcacataa acaggttcca taccagtcct
      961 gaccgcatca ggacgccagt aaataaaatc tcgtagtcca ccggcatcag agacaagagt
     1021 aatcgtcgtt accggtattt cgactggttt gtactggtgt gagcggacaa aactactgta
     1081 cgtctctcat acacgctcgt aagcggccct cacggcccgc taacgcggag atacgccccg
     1141 actgcgggta aacccttgtc gggaccactc cgaccgcgca cagaggcatt ttcatcgctg
     1201 acaggccgca tggcctcgca gagccggagg gggactggtg atattcggac gaccgtaccg
     1261 gcttgcgccg ggcttcggcg gttttacacc tgtgctacat gtaaccaccg tatgccgcct
     1321 tatatgcgct gactcgcatt atttgccttg ttccgtctga aagtaatcac tatgattaaa
     1381 tatgattaac aactaatcgg gtatgcaaat gaaaaacaat accgcacaag caacaaaagt
     1441 aattaccgcg catgtgccat tacctatggc tgataaagtc gaccagatgg ccgccagact
     1501 ggaacgctcc cggggctggg ttatcaaaca ggcgctttct gcatggcttg cccaagagga
     1561 ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg acatccggac aggttatcga
     1621 ccatcaggct gtacagtcct gggcggacag cctcagtact gaccatctgt taccggtgcc
     1681 acgctgatgg aactgaagtg gaccagtaag gcactttctg atttggcgcg gttatatgat
     1741 tttctggtgc tggccagtaa acctgctgcc gccagaacgg tgcagtccct gacacaggcg
     1801 ccggtcattc tgttaactca tccacgtatg ggagaacagt tgtttcagtt tgaacccagg
     1861 gaggtcagac ggatttttac tggcgagtac gaaatccgtt acgaacttac tggccagact
     1921 atttatgtat tgcgcctgtg gcacacacga gagaacaggt agattgggtg tggaggcaac
     1981 tttaacatag cctgcaggta ttaattctgg agcattgtta tcaggccaga tattttgcat
     2041 tccattaaat aatcttgtag gagataaaat aaattacgat gaaataacga aaattaccgc
     2101 tcgtgactgg cggcaatatc gcgctccggg ctggcaaata acccatcaga aacgttattg
     2161 ccaga
//
LOCUS       DADLEE010000239         2162 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000239 DADLEE010000000
VERSION     DADLEE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.246"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            80..259
                     /locus_tag="JQH51_004243"
     CDS             80..259
                     /locus_tag="JQH51_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078345.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="JQH51_004244"
     CDS             311..505
                     /locus_tag="JQH51_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078346.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="JQH51_004245"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="JQH51_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DADLEE010000240         2151 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000240 DADLEE010000000
VERSION     DADLEE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.247"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JQH51_004246"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JQH51_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY6078347.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JQH51_004247"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JQH51_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JQH51_004248"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JQH51_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JQH51_004249"
     CDS             1905..>2151
                     /locus_tag="JQH51_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078348.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADLEE010000241         2134 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000241 DADLEE010000000
VERSION     DADLEE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.248"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..179)
                     /locus_tag="JQH51_004250"
     CDS             complement(<1..179)
                     /locus_tag="JQH51_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078349.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSVSGT"
     gene            complement(396..929)
                     /locus_tag="JQH51_004251"
     CDS             complement(396..929)
                     /locus_tag="JQH51_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY6078350.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(995..1624)
                     /locus_tag="JQH51_004252"
     CDS             complement(995..1624)
                     /locus_tag="JQH51_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078351.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1628..1843)
                     /locus_tag="JQH51_004253"
     CDS             complement(1628..1843)
                     /locus_tag="JQH51_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY6078352.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          546 a          567 c          471 g          550 t
ORIGIN      
        1 gtcccggata ccgagaacgt ttcgtccagc aactggagag agttcgacgg tggcatcatt
       61 gcccatgccg gaggcgctgg aggtttcggc tgaggatggc acagggcatt ttcctttgac
      121 gagcacccga ccaccattat caagcttgcg ccgaagagca tcattttcag ctttcgcatc
      181 agctaactcc ttcgtgtatt tagcatcgag tgcatcagca tcacgctggc gctgctgcat
      241 gtcagtaatg gttgcgttcg ccagcttcag ttctccggcg tttttgtcgc gctgctcttt
      301 gtaggtaatg gcgttatcac ggtaatgatt aacagcccat gacaggcaga cgatgatgca
      361 gataaccaga gcggagataa tcgcggtgac tctgctcatt gatctatccc ccaacaggct
      421 aatgcgcttt cctggtcacg acgaataacc tgtccatagc agttatttga acgtatgcgg
      481 caatcgcgcc caacatcttt tatccaccag cgaatcgcct cgcatgcgcc cttacgatca
      541 ccggcattca gccgcttata aaacgtcgac gggaaacact taccggggcc aatgttatag
      601 gggcaaaatg acgctatacc cgctttctgg ggttcggtca gaggtacttt aatattgcgc
      661 tccacccatg ccagcgcctt atcacgttca atagcgttaa cctggtcaca tttttccttc
      721 gacagcttca ttcccggtat gacaggctta ccatccacca ttgtggcacc acgacagatg
      781 gtccatatgc cagaaccatc acggtatgcc gttgtgtgat taccctcttt ttcgttcaga
      841 aactggtcaa gtatctgggg agcagacgcg cctgcggcaa tcagcgccag aacagcagcc
      901 gacaggccgt atttgatttt tgcgctcatg gatatttatc aggatttatc ggcaacaaat
      961 aacgagccaa cttatatacg ccttttaaga taagtcagcc ccggctggat ccagtcatca
     1021 ggctctttct taaaggggga gtattgaaat cacgaagaag agcctcccgc accgcagcat
     1081 ccatgtctgc accactggca agtgcttcaa tctctgccgc cacctgcaaa taccccatgc
     1141 aacggccaat gcgtttcata actctggtgt tttcgtcttt caggcatctg atagcgagat
     1201 taataatttt caggcagtga tgatgcgatg cccggacagg ttttccgttc tcataaacga
     1261 cgaactcacc ctcttcacag gaaaccctga aaggccgatg gggttcctgg tctttatccg
     1321 gtgcagacgc aataaaatca cttttatgct cttcctctcc ggcactgtta accgtaatga
     1381 cgtactcagg ccgcaggtaa gccgcctctt tttctgacaa tgcggccggt aaaaactttt
     1441 cctgcaaaaa cttcataaaa gtctcgtgag cgactttgtg gcagtaatcg taaatcgccg
     1501 cataatgctc atcgcggcgt ttgttttcat cttcagaact caccaacgcc gacaattttt
     1561 tattaagctc agtgatttca ttttccaggt gattgaatcg ctgattcatt tcttcatgct
     1621 tcattattta ctctccccgt gcagccttac gcttgtcttc tcttattttg aaatacagat
     1681 tcgtcagata agtcagaagc cccagaagca gacttcccag cacaccaatc gcagcccact
     1741 gtgatggcct gacctgatcc aaccactgca aaaaccagta gccggcactg ccggcggagg
     1801 tgccgtaggc aatgcccgtt gaaattttgt ccatggattt catagcctca cctccgcaaa
     1861 taacggatgg cgtagtttta cactgagaaa tgaaagggat ttgaaaagaa aaaaacgcaa
     1921 aagcgggcga aacgatatat acagtaagga aagcactcta tccaacaaac cacccacagt
     1981 taatcggaat aaaagcagag tgcttatgaa tgatcgcacg cccgaaggtt agtatttctg
     2041 cacagcaatt ttgcaaaaaa aggcgatcat tcataactta aacgtctttc agtcactccg
     2101 ggatttccca tcatcgcaga ctgaaagact ctga
//
LOCUS       DADLEE010000242         2132 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000242 DADLEE010000000
VERSION     DADLEE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.249"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..132
                     /locus_tag="JQH51_004254"
     CDS             <1..132
                     /locus_tag="JQH51_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY6078353.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..483
                     /locus_tag="JQH51_004255"
                     /pseudo
     CDS             319..483
                     /locus_tag="JQH51_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            977..1555
                     /locus_tag="JQH51_004256"
     CDS             977..1555
                     /locus_tag="JQH51_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078354.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1664..1922)
                     /locus_tag="JQH51_004257"
                     /pseudo
     CDS             complement(1664..1922)
                     /locus_tag="JQH51_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1906..2132
                     /locus_tag="JQH51_004258"
                     /pseudo
     CDS             1906..2132
                     /locus_tag="JQH51_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          670 a          378 c          522 g          562 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat
     1021 atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc
     1081 aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga
     1141 caatgagata acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc
     1201 cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat
     1261 tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac
     1321 tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat
     1381 tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg
     1441 cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga
     1501 aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta
     1561 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
     1621 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
     1681 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
     1741 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
     1801 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
     1861 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
     1921 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
     1981 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
     2041 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
     2101 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DADLEE010000243         2120 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000243 DADLEE010000000
VERSION     DADLEE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.250"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            334..557
                     /locus_tag="JQH51_004259"
                     /pseudo
     CDS             334..557
                     /locus_tag="JQH51_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            666..932
                     /gene="ospE1"
                     /locus_tag="JQH51_004260"
     CDS             666..932
                     /gene="ospE1"
                     /locus_tag="JQH51_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY6078355.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1322..>2120
                     /locus_tag="JQH51_004261"
     CDS             1322..>2120
                     /locus_tag="JQH51_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6078356.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          703 a          451 c          361 g          605 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccaaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttccaca aaaacaagaa aatatcatcc
      721 ttgaagtatc aaatcctgta ttattatctt ctactgttac tacagatgga tatactgttt
      781 tcaataaaaa agcagctata tacgaattac aaatacctgc tgccagccgg acgaaaacgt
      841 tgaaattcac tgctacagaa atgcaatggt tgacaaagat aaatgaagcc ggaattgatg
      901 aaaaacaaag ccaacggtat tctgatttct gaagttaatt tcagactcgt ttaagctcca
      961 ttttcaaaaa aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag
     1021 agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt
     1081 atcgtgatat attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg
     1141 accttaaata cctttttatg gctgaattac cattctcagg aataacagca gactccttcc
     1201 catagacggc ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa
     1261 aattaataag ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat
     1321 aatgattaaa tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa
     1381 tgtatactcg ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt
     1441 ttatttaaaa acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
     1501 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
     1561 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
     1621 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
     1681 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
     1741 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
     1801 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
     1861 aaacagatta gacagcattc ccccctttcc agataagctt gaagggctgg ctctggctaa
     1921 taattttata gaacaactac cggaattacc ttttagtatg aacagggctg tgctaatgaa
     1981 taataatctg acaacacttc cggaaagtgt cctgagatta gctcagaatg ccttcgtaaa
     2041 tgttgcaggt aatccattgt ctggccatac catgcgtaca ctacaacaaa taaccaccgg
     2101 accagattat tctggtcctc
//
LOCUS       DADLEE010000244         2092 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000244 DADLEE010000000
VERSION     DADLEE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.251"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="JQH51_004262"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="JQH51_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY6078357.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /locus_tag="JQH51_004263"
     CDS             1351..2016
                     /locus_tag="JQH51_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY6078358.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DADLEE010000245         2085 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000245 DADLEE010000000
VERSION     DADLEE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.252"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..797
                     /locus_tag="JQH51_004264"
                     /pseudo
     CDS             1..797
                     /locus_tag="JQH51_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1236..2073
                     /locus_tag="JQH51_004265"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="JQH51_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DADLEE010000246         2077 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000246 DADLEE010000000
VERSION     DADLEE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.253"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(11..277)
                     /gene="ospE2"
                     /locus_tag="JQH51_004266"
     CDS             complement(11..277)
                     /gene="ospE2"
                     /locus_tag="JQH51_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY6078359.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(459..860)
                     /locus_tag="JQH51_004267"
                     /pseudo
     CDS             complement(459..860)
                     /locus_tag="JQH51_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1289..2077
                     /gene="virF"
                     /locus_tag="JQH51_004268"
     CDS             1289..2077
                     /gene="virF"
                     /locus_tag="JQH51_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY6078360.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          640 a          368 c          386 g          683 t
ORIGIN      
        1 gaaattaact tcagaaatca gaataccgtt ggctttgttt ttcatcaatt ccggcttcat
       61 ttatctttgt caaccattgc atttctgtag cagtgaattt caacgttttc gtccggttgg
      121 cagcaggtat ttgtaattcg tatatagctg cttttttatt gaaaacagta tatccatctg
      181 tagtaacagt agaagataat aatacaggat ttgatacttc aaggatgata ttttcttgtt
      241 tttgtggaag gcaagggaat atagtttgtg taagcatatt gcttccttat gttatatttt
      301 agtaaagaat ttatttttta cattatagtc ggctaaatga agtttactgt aaaaaaacga
      361 tacttttttc gtaagcgatg agatgtggag ccagaaaaat ggctgcgtta cgtcattgaa
      421 ctgtccttga cccacgatat tcagacagtc gttgtatctt aagttgacat tacccgctga
      481 cgtcgatatt cccttggtga gagataaccc aacgcactat gcggatggtt ttcattgtaa
      541 tgctcgatcg ccactgcaag gttatgcaat gccgttcttg gtttcggcat gaacgcgatg
      601 cagtcttcct tcatcgtttt catgaaccgt tctgctattc cattactctg agggctactg
      661 actgccgtcg tgcagggctc cagattcaac tctctggcga actgccgtgt ttcatgagcc
      721 gtataagccg aaccgttatc ggccactgga ttggccgttc cggtccttta tcgccatatc
      781 gctcctgaca tgacatcctg caccgttgct ttgtcataat ctcccgtgcc tgctgcccag
      841 tctatggctt cacgatcgca atatggaacg taacgctgtc atcgggagaa tcgtcaatgc
      901 ggcattcggt taacgcttac caatatcaac accaaccgga tttgtggagc ctccagtctg
      961 aaggggcttt atgcgttccg tataggatat tatgatgctg gagttttgca aagctccact
     1021 cctttccgaa catttacaca gatattgcct aagaaaagtg aaaccataat atggttatag
     1081 tccctttcag tgcaaatact tagcttgttg cacagagaaa tagaagctgc ataagctctt
     1141 tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt acttaatcta tcttaataac
     1201 ggaaagattt tgtatacaat cactgttaca gaaatttctt agttactctg taaacactaa
     1261 atatagtttg gtatattctg ttgaatttat gatggatatg ggacataaaa acaaaataga
     1321 tataaaggtt cgcttgcata actatattat tttatatgca aaaaggtgtt caatgacggt
     1381 tagctcaggc aatgaaactt tgactatcga tgaagggcaa attgctttta tagagcgaaa
     1441 tatacaaata aacgtctcca taaaaaaatc tgatagcatt aatccatttg agattataag
     1501 ccttgacaga aatttattat taagcattat tagaataatg gaaccaattt attcatttca
     1561 acactcctat tctgaggaga aaagggggtt aaacaaaaaa atattcctcc tctctgagga
     1621 ggaggtttct atcgatttgt tcaaatctat aaaagagatg cctttcggca aaagaaagat
     1681 ctatagttta gcttgccttt tatcagctgt ttctgatgag gaagctttat atacttcgat
     1741 atcgatagct tcttctctta gtttttctga tcagataagg aagattgttg aaaaaaacat
     1801 cgagaagaga tggcgtcttt ctgatatttc aaataacttg aatttatcag aaatagctgt
     1861 tagaaaacga ttggagagtg aaaaattaac atttcaacaa atccttcttg atattcgcat
     1921 gcatcatgca gcaaagcttt tattgaatag tcaaagctat attaatgatg tatcaagact
     1981 tatcggaata tcaagcccat cttattttat aaggaaattt aatgaatatt atggtataac
     2041 tccaaagaaa ttttacttat atcataaaaa attttaa
//
LOCUS       DADLEE010000247         2071 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000247 DADLEE010000000
VERSION     DADLEE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.254"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..259)
                     /locus_tag="JQH51_004269"
     CDS             complement(<1..259)
                     /locus_tag="JQH51_004269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078361.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(979..1218)
                     /locus_tag="JQH51_004270"
     CDS             complement(979..1218)
                     /locus_tag="JQH51_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY6078362.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1413..1770)
                     /locus_tag="JQH51_004271"
                     /pseudo
     CDS             complement(1413..1770)
                     /locus_tag="JQH51_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="JQH51_004272"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="JQH51_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          581 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DADLEE010000248         2053 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000248 DADLEE010000000
VERSION     DADLEE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.255"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(26..914)
                     /locus_tag="JQH51_004273"
                     /pseudo
     CDS             complement(26..914)
                     /locus_tag="JQH51_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          557 a          477 c          476 g          543 t
ORIGIN      
        1 agtctaaaaa ctggtattaa gactatcact taaataagtg atactggttg tctggagatt
       61 cagggggcca gtctagcagc tgctctacgg tcatattttt aattgcgctc cggtttacaa
      121 gagcccaccc ctgtttttcc agataaaact ggaaagtatc cagggtacag accagtgcac
      181 cgtcaggaac agtttcggtg aatctgatat tgccgtgttc gtcgaagtgc acaaccagag
      241 tgcgaccgtt acccggaatc atcttgtcag cggacggggt attattctgg tgcagttctg
      301 cctccatgcg gtcgaacatc agcgatgtag gcctctttga atgcagcggc ttttttccca
      361 gtgaagccca tcaccaggaa aacgaagccg tttttggtga tttggtacat tgggcgtttt
      421 tcgcctttgg tgtcggtgta ggtgacgtcc tcaaaattga gggcgttaaa ttcaggtgag
      481 cattcagtat ttgcgatggc acgtaacaca ttgtcgtgtc gtttgtggaa gaactctgca
      541 accgcaacag acgtagtgac agcgcgaccg ttttcgatgg ttacgtcagg gtgagaaagg
      601 gcagggatag tagccatgat ggcatcctcg agtgataagt taattaactc accaccgagg
      661 ttttccacga ccataagggt ggtgagacgt acaggggtgg aaataccggt cactcgagaa
      721 cccggccagt cttgcgactg ccctgcacat cccaccataa tttgaatgga gctgtgcatt
      781 acgcataaaa aaaccgcttc agcgcggcta tgcgctcgag taacgttcgg gtttccacgc
      841 ccggcccccg ttttatgagg tgcagatgca ctataattcc acccgttctg gttttcaata
      901 gctacattca acattttctc ttacctttca tcaccgaagt gaactttgtt gatgcggtgc
      961 ctggtgcctc caggtgacgt taaccagtta acaattaacg ccggatacag ggaaacccat
     1021 aaccacccga tacgtgggaa ctaatcgctt tttaactgtt ccgcgtgcgc tcagccgcat
     1081 tcaccgcatc acaaaattca ctttaaaaag ggcggacatc agccagcaat gaaactgatg
     1141 ccgccaaagg taccaatcaa catggagtgt tgtggcgtgg ttgtcactta agcgtatggt
     1201 caacctgaca acccggtgtc ctcaacgggg aaggaataac tccgccatac ttaccgccgc
     1261 gtcatttcgc gttgtgtgcc tgcttttaac tacgtcaggc gaggtggttc ctgttattcc
     1321 ccaacaacaa gaaatttgta taatctgaat accccaacaa taagaagagt gagcaagtga
     1381 tgtattagcg atgtcagctc tcaaagagac tgcatcaatg ttaaaattat tcaatgaagc
     1441 aaaaactcag gctgaggttg atcgcgctgt gtctgaaata aactctaagt tgtcaagcat
     1501 tcagcttgaa tatttgtcat ctcattgatg agctgacaat ctctaaaagc aatgaggctg
     1561 cgctcaaagc aaaactcacg gagatagaaa agtttaagtt ccaaagtgaa gactgcgaac
     1621 ctcgctaatt gattactgga acgattgtgt atgccagaca gtgcaatact agctctggca
     1681 agattaccat gtatttttgt ccagaatgtt ttgcgaagag agcaatatct atattgcaac
     1741 ctttccctat cgaggaaggc gataaatata cgaaaactca atgcccctcc tgtaaaaatg
     1801 agtatctcat ggactacaat ttcaataatg atttttgagt cacggtattc agatttttaa
     1861 agaacatgcc ggatactcac ccgtgtccgg cgcacgcact ccacctcacc cgtggagaac
     1921 tccttaatta ccaaccttgg cttcgttggt tagccattaa cgctggtatg taatcattct
     1981 ggcaatgctt aatttgaagt ggcacactga atttggccac ctgaacagag gtgatatgct
     2041 cacctcagaa caa
//
LOCUS       DADLEE010000249         2044 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000249 DADLEE010000000
VERSION     DADLEE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.256"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(55..324)
                     /locus_tag="JQH51_004274"
     CDS             complement(55..324)
                     /locus_tag="JQH51_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6078363.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            381..1049
                     /locus_tag="JQH51_004275"
     CDS             381..1049
                     /locus_tag="JQH51_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY6078364.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1365..>2044
                     /locus_tag="JQH51_004276"
     CDS             1365..>2044
                     /locus_tag="JQH51_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6078365.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          614 a          447 c          381 g          602 t
ORIGIN      
        1 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
       61 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
      121 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
      181 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
      241 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
      301 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
      361 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
      421 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
      481 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
      541 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
      601 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      661 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
      721 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      781 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
      841 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      901 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      961 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
     1021 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
     1081 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
     1141 ggcagaacgt tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac
     1201 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
     1261 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt
     1321 ccccagtatc acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata
     1381 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
     1441 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
     1501 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
     1561 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
     1621 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
     1681 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
     1741 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
     1801 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
     1861 cagccagaaa tgctttggaa gtaataccag attttccaga aagagatgat catattataa
     1921 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
     1981 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
     2041 tgtt
//
LOCUS       DADLEE010000250         2036 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000250 DADLEE010000000
VERSION     DADLEE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.257"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="JQH51_004277"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="JQH51_004277"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY6078366.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2036
                     /locus_tag="JQH51_004278"
     CDS             1647..>2036
                     /locus_tag="JQH51_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6078367.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEAC"
BASE COUNT          554 a          427 c          518 g          537 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgt
//
LOCUS       DADLEE010000251         2035 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000251 DADLEE010000000
VERSION     DADLEE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.258"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..362)
                     /locus_tag="JQH51_004279"
     CDS             complement(<1..362)
                     /locus_tag="JQH51_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY6078368.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JQH51_004280"
     CDS             complement(429..1643)
                     /locus_tag="JQH51_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY6078369.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          493 c          414 g          495 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatgc
//
LOCUS       DADLEE010000252         2034 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000252 DADLEE010000000
VERSION     DADLEE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.259"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            7..564
                     /locus_tag="JQH51_004281"
                     /pseudo
     CDS             7..564
                     /locus_tag="JQH51_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1084..>2034
                     /locus_tag="JQH51_004282"
     CDS             1084..>2034
                     /locus_tag="JQH51_004282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY6078370.1"
                     /translation="MKETESSYNKKFNSDYKSNNQQTSFDQPDWKTGVFKFDTLHLNN
                     ADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGE
                     TKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDIS
                     GELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITL
                     GQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRL
                     ETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQAD"
BASE COUNT          747 a          356 c          400 g          531 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagcaaccat tactctggga
     1741 cagcctgaaa ctgaaacacc gactatatcg tctgcttatc aggcatgggc agagactctt
     1801 ttgtatggct ttgataccgc ctatcgaggc gcaataacag cccccaaagc tacagttagc
     1861 atgaataatg cgatctggca tctaaatagc cagtcatcaa ttaatcgtct agaaacaaaa
     1921 gacagtatgg tgcgttttac tggtgacaat gggaagttta caacccttac agtggacaac
     1981 cttactatag atgacagtgc atttgtgctg cgtgcaaatc tggcccaagc agat
//
LOCUS       DADLEE010000253         2031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000253 DADLEE010000000
VERSION     DADLEE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.260"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1824)
                     /locus_tag="JQH51_004283"
     CDS             complement(<1..1824)
                     /locus_tag="JQH51_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078371.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          485 a          580 c          431 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagcg c
//
LOCUS       DADLEE010000254         1969 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000254 DADLEE010000000
VERSION     DADLEE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.261"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            240..509
                     /locus_tag="JQH51_004284"
                     /pseudo
     CDS             240..509
                     /locus_tag="JQH51_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..618
                     /locus_tag="JQH51_004285"
                     /pseudo
     CDS             506..618
                     /locus_tag="JQH51_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="JQH51_004286"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="JQH51_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY6078372.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DADLEE010000255         1935 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000255 DADLEE010000000
VERSION     DADLEE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.262"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(207..419)
                     /locus_tag="JQH51_004287"
     CDS             complement(207..419)
                     /locus_tag="JQH51_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY6078373.1"
                     /translation="MLCTCRLASYMPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRK"
     gene            complement(590..1255)
                     /locus_tag="JQH51_004288"
     CDS             complement(590..1255)
                     /locus_tag="JQH51_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY6078374.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1424..1837)
                     /locus_tag="JQH51_004289"
     CDS             complement(1424..1837)
                     /locus_tag="JQH51_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078375.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPIKEDDPRNQ
                     VNFLYKPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEEQERRRNIEKLIQIERNAKARIRRATKSRTE"
     gene            complement(1838..>1935)
                     /locus_tag="JQH51_004290"
     CDS             complement(1838..>1935)
                     /locus_tag="JQH51_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY6078376.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          466 a          502 c          430 g          537 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctccccg ccctttcggg cggtctcctg atgatttgag ggtgcagaaa
     1381 tccctccggt taaggattaa atttttaaca gtgctaaatt taattattca gttctggatt
     1441 ttgtcgccct gcgtatccgc gctttcgcgt tacgctcaat ctgtatcagc ttttctatat
     1501 ttctccgcct ttcctgttcc tcctggcgca atagccttac atcatctgcc agtctggttt
     1561 ctctttttgc cacagagagc atccagtcaa acggctccac aaccgcaccg cagattttac
     1621 agcggacctg acgctctttt tcgtcaaccc gtacagaggc gtgatggcaa tatggtcttt
     1681 ccgatggctt ataaagaaaa ttaacctgat tacgagggtc atcctctttt atcggaaata
     1741 aaacgatatt gcttaactca tcttctggtt ttatttccat gctcctctcc tttgatgcga
     1801 atgccagcgg taattgaagc ctgatagcta atttcattca cagtatcgcc tcctgaaaat
     1861 taccctgata gaaagccagt acacgctgca tagcttcgct cttccggcac tcgcgacaga
     1921 ttatattcag gcgcc
//
LOCUS       DADLEE010000256         1913 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000256 DADLEE010000000
VERSION     DADLEE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.263"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(53..1702)
                     /gene="treF"
                     /locus_tag="JQH51_004291"
     CDS             complement(53..1702)
                     /gene="treF"
                     /locus_tag="JQH51_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY6078377.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DADLEE010000257         1849 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000257 DADLEE010000000
VERSION     DADLEE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.264"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..257)
                     /locus_tag="JQH51_004292"
     CDS             complement(<1..257)
                     /locus_tag="JQH51_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078378.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="JQH51_004293"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="JQH51_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY6078379.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="JQH51_004294"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="JQH51_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY6078380.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="JQH51_004295"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="JQH51_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY6078381.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /locus_tag="JQH51_004296"
     CDS             1253..1504
                     /locus_tag="JQH51_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078382.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="JQH51_004297"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="JQH51_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DADLEE010000258         1836 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000258 DADLEE010000000
VERSION     DADLEE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.265"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..344)
                     /locus_tag="JQH51_004298"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="JQH51_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="JQH51_004299"
     CDS             complement(344..1264)
                     /locus_tag="JQH51_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY6078383.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1836
                     /locus_tag="JQH51_004300"
     CDS             1402..>1836
                     /locus_tag="JQH51_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY6078384.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          459 a          447 c          424 g          506 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtc
//
LOCUS       DADLEE010000259         1832 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000259 DADLEE010000000
VERSION     DADLEE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.266"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..180)
                     /locus_tag="JQH51_004301"
     CDS             complement(<1..180)
                     /locus_tag="JQH51_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY6078385.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="JQH51_004302"
     CDS             complement(321..875)
                     /locus_tag="JQH51_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY6078386.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="JQH51_004303"
     CDS             complement(872..1162)
                     /locus_tag="JQH51_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY6078387.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="JQH51_004304"
     CDS             complement(1162..1761)
                     /locus_tag="JQH51_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY6078388.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFGAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          421 g          469 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgccaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DADLEE010000260         1809 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000260 DADLEE010000000
VERSION     DADLEE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.267"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            173..1198
                     /locus_tag="JQH51_004305"
     CDS             173..1198
                     /locus_tag="JQH51_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY6078389.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="JQH51_004306"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="JQH51_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DADLEE010000261         1798 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000261 DADLEE010000000
VERSION     DADLEE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.268"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1312)
                     /locus_tag="JQH51_004307"
     CDS             complement(<1..1312)
                     /locus_tag="JQH51_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY6078390.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINN"
BASE COUNT          537 a          314 c          298 g          649 t
ORIGIN      
        1 cattattgat aaaatattct agcactttat agttggcacc atctttacta agtaagtatt
       61 caacatcata cagattacaa tatctattgt ttttcatttt ctccatctct gcgacatctt
      121 gttttgtaaa attaaattta gatagtaaat aaagtgctat atcagctttt ttattgccta
      181 aagaataagc aagggcctga tgagccattt ttttatcagt cactttattg ttaatctctt
      241 cataattaga tgctttaatc gcatcctcca aagatatgtc tctgagtttt atttttttga
      301 agttatctgt acttagcatt cttggtatat gatactcaag ctgaaacaca aagtttataa
      361 ttctatccag actaacagga tcaatatttt tgttataaca aaactctctg aatccttgat
      421 ctttactctt tcttataaaa tcaataagat gcaacccaat tccaagcctc atatctttag
      481 tgttaaatat tggaacatca gttttcccct gattaccatg cacatacctg ctaacttcat
      541 ccacaacctc tttaaagtta tctaagaaaa atgattggtg ctcatggtaa aaaacaggtg
      601 gaatagcatt atcaaagtga tcggttaatg tcatatatcc atatggagag tcatgatcaa
      661 tgatatacgc attggcgcca aaatctacag tatgatgtgt tttatttagt gggagttttt
      721 cttgatgatt ggaaatttcg accccaaaaa aaacaaaatc gtggttagag atttctttaa
      781 tatcctcaga gtatgtattc ttatgtgttt gtatattttc agactgaatt ttatcaaagg
      841 atttaatatt aagaatatta tttgacagat tagagttact ttgatgtctg aagttaaaat
      901 tagaattaat aataatagaa agtaagtgac gttcgtctga agaaagagga tgaacttttt
      961 gtattatctc agagatatag tgcctggaca ccttatcgag atgctcaagt gaccgaaatg
     1021 atgaccgtgc tccagatggc gcttcaagag aaagatttag tgatttgaaa ctggtccctt
     1081 gagaaaacat tttgctataa ctttgcgctg ctatagactt ccttaaaaag tttttcacag
     1141 tattagcaca gtgatctaaa tgtttttcac ttatgccaga agaagatgag tttgttacgc
     1201 gtaatacatt tttttgtaat gcttttgttg ctttattcgg attcgttttt ccatcaacaa
     1261 gatcaatatt attctgaaca ttaatatgat ttactgcttc aggtattttc atcccccccc
     1321 cccaattatg tgcgttttta tctgtggtta tttagataac attatctaac tggacacttt
     1381 cttgtatgta ttatagctaa atgaacagat tttaatatgt tgtatggttt tcactctgaa
     1441 atcccctgaa tttaatcacg tttcctttac tgaaggcaat tttaaccgta gtatcaatta
     1501 ttaacccttg ggcttgatat ccctctcctc ttctgggcac gggggatgtc agattactta
     1561 ttacagaaac caaaagaatt gtaagcgtca tctcagtgtc gtctatttca atgtctgatg
     1621 attggcgatg atagagtcct cttataacgt taatggactc tatcaatgtc agattctctt
     1681 cgcccccgca ggattcgtgc ctcttactct atgactctat ggagtttaag cttgatctta
     1741 ttgaaaaatc ttaccagcta ggggcctgcg tggcccaact ggccagagag tacgggat
//
LOCUS       DADLEE010000262         1759 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000262 DADLEE010000000
VERSION     DADLEE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.269"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..263)
                     /locus_tag="JQH51_004308"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JQH51_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /locus_tag="JQH51_004309"
     CDS             complement(378..1016)
                     /locus_tag="JQH51_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078391.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="JQH51_004310"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="JQH51_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          433 c          319 g          505 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacga ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DADLEE010000263         1749 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000263 DADLEE010000000
VERSION     DADLEE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.270"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            346..1302
                     /locus_tag="JQH51_004311"
     CDS             346..1302
                     /locus_tag="JQH51_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6078392.1"
                     /translation="MVWVGKPTVIVNWLTSPGGTRHRINKVHFGDERKEKMLNVAIEN
                     QNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLCVKHSHIQIMVGRAGQPQGWPVS
                     VVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATILTLSHPDATIENGRAVTTSVAV
                     AEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTDAKGEKRPMYQITKNGFVFLVMG
                     FTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVVHFDKFGNVEFTET
                     VPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLLSLK"
     gene            1319..1600
                     /locus_tag="JQH51_004312"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="JQH51_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          500 a          398 c          411 g          440 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagcat
//
LOCUS       DADLEE010000264         1744 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000264 DADLEE010000000
VERSION     DADLEE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.271"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..353)
                     /locus_tag="JQH51_004313"
     CDS             complement(<1..353)
                     /locus_tag="JQH51_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078393.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="JQH51_004314"
     CDS             complement(350..940)
                     /locus_tag="JQH51_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078394.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="JQH51_004315"
     CDS             complement(927..1298)
                     /locus_tag="JQH51_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078395.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /locus_tag="JQH51_004316"
     CDS             complement(1310..1711)
                     /locus_tag="JQH51_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY6078396.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          530 c          406 g          415 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DADLEE010000265         1717 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000265 DADLEE010000000
VERSION     DADLEE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.272"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(110..577)
                     /locus_tag="JQH51_004317"
     CDS             complement(110..577)
                     /locus_tag="JQH51_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY6078397.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="JQH51_004318"
     CDS             complement(643..882)
                     /locus_tag="JQH51_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY6078398.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="JQH51_004319"
     CDS             complement(1017..1622)
                     /locus_tag="JQH51_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY6078399.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DADLEE010000266         1644 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000266 DADLEE010000000
VERSION     DADLEE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.273"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..430
                     /locus_tag="JQH51_004320"
     CDS             <1..430
                     /locus_tag="JQH51_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078400.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="JQH51_004321"
     CDS             550..723
                     /locus_tag="JQH51_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY6078401.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="JQH51_004322"
     CDS             823..1644
                     /locus_tag="JQH51_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY6078402.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          427 a          416 c          420 g          381 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctga
//
LOCUS       DADLEE010000267         1601 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000267 DADLEE010000000
VERSION     DADLEE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.274"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(333..479)
                     /locus_tag="JQH51_004323"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="JQH51_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="JQH51_004324"
     CDS             533..715
                     /locus_tag="JQH51_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY6078403.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="JQH51_004325"
     CDS             complement(816..1145)
                     /locus_tag="JQH51_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY6078404.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="JQH51_004326"
     CDS             complement(1317..>1601)
                     /locus_tag="JQH51_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078405.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DADLEE010000268         1599 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000268 DADLEE010000000
VERSION     DADLEE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.275"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..704)
                     /locus_tag="JQH51_004327"
                     /pseudo
     CDS             complement(1..704)
                     /locus_tag="JQH51_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(752..1324)
                     /locus_tag="JQH51_004328"
     CDS             complement(752..1324)
                     /locus_tag="JQH51_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HAY6078406.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          338 c          319 g          467 t
ORIGIN      
        1 actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga attagcaggt
       61 aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc atggccatca
      121 acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat cagatttcgc
      181 ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg gatatttgct
      241 ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt cgtcgctttg
      301 ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc gcgatcatca
      361 ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga aggttctaat
      421 cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt attaaatgga
      481 tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac agaaatagat
      541 ttatcagcat tattacttaa gtaaaccacc cgcgtactac caatgacaac gcctgcataa
      601 gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa cttacattaa
      661 actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac tccctggcca
      721 aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca gtaaattgtg
      781 aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag cgtgcaataa
      841 acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg ttataaagtt
      901 taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt gccgcaatcg
      961 cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta cccactttca
     1021 ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct tgagtgtacg
     1081 ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga ctacctacac
     1141 ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct gtatcggagg
     1201 taacttcaca agtagtgtcg acaatttcac cgacgaagtt aacaacacca tcttctgcca
     1261 gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata atattacgct
     1321 tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt atattaataa
     1381 gtgtgttttt gattcaaagg aatagtacac caaagagacg gctttatcta aaaataatat
     1441 taattacgca aaaataaaat atagatttat tgtttaaata ttatttaaac agtaaaaaat
     1501 caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa agatagatga
     1561 atttattgat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DADLEE010000269         1580 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000269 DADLEE010000000
VERSION     DADLEE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.276"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="JQH51_004329"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="JQH51_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HAY6078407.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1580)
                     /locus_tag="JQH51_004330"
     CDS             complement(1269..>1580)
                     /locus_tag="JQH51_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAY6078408.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          481 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac
//
LOCUS       DADLEE010000270         1556 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000270 DADLEE010000000
VERSION     DADLEE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.277"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            624..1223
                     /locus_tag="JQH51_004331"
     CDS             624..1223
                     /locus_tag="JQH51_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY6078409.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          353 g          422 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggct
//
LOCUS       DADLEE010000271         1439 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000271 DADLEE010000000
VERSION     DADLEE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.278"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(196..885)
                     /locus_tag="JQH51_004332"
     CDS             complement(196..885)
                     /locus_tag="JQH51_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY6078410.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(882..1241)
                     /locus_tag="JQH51_004333"
     CDS             complement(882..1241)
                     /locus_tag="JQH51_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY6078411.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
BASE COUNT          353 a          405 c          293 g          388 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttaccgc
      421 ctgcgccgcc agccagatta attttttcag gcgttcctgt gttttctctg caattttcct
      481 agtgccagaa tgagccttaa actcttccca cgcccagtgg gttatggtga tctgatactc
      541 ccagcaaata cttccgctgt aacaccacaa caaccaggct ttatagtgct cttcaagaga
      601 cagaacagcc cgccgccacg atgatgtcga aaacgcaacc tgactgacca gaggaattga
      661 cgcccccttc gccagtgatt gttttcccgg gattggtgga ttatcccgcg ttatcatttt
      721 tccggtcacc tcatcgcggt accggatttt tttacgcctg taacgccctg tatcgaacat
      781 ggcattctcc tgccaggctt caagctgacc ttttgtcgcc ccactcaaat cagcggtggc
      841 gataatgagc tgctcacgaa caaactgtaa atactggtta ttcatgcaca ctccagctct
      901 gtgattttta tccccaaccg accaccagga acaggcagtc cgcgcacaat attggtttca
      961 tcaaactgct cgtcgtcgat aagcaacccc gcatgtgtca gtgcatccag tggtgctttc
     1021 aggatattgt ccaggtcacg acgccgctta tccggtggct ctgcaataat ctttatcgcc
     1081 agtcttccgg acaggcttaa tttcagtcgc tgctgacgga caataagcgc cacatcccgg
     1141 cgataacgct caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg ccagtaagta
     1201 ttcaccgtcg gcggataagg taaaataaac tcatggcgca tcagcgcagc acctcctgca
     1261 ccagcttttc aaactttccg actctggttt ccagctctgc cacacagtcc accagctcat
     1321 ctactgcttt ctgtgcgcga tgcttcgcct gcatcagttc ccgaagcgcg ggtaccatat
     1381 tgaggtagcc tgagtttaac ggacactcct tcctgaaata gaatggcatc agaaggagc
//
LOCUS       DADLEE010000272         1434 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000272 DADLEE010000000
VERSION     DADLEE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.279"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..253
                     /locus_tag="JQH51_004334"
                     /pseudo
     CDS             1..253
                     /locus_tag="JQH51_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1434
                     /locus_tag="JQH51_004335"
     CDS             746..>1434
                     /locus_tag="JQH51_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY6078412.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          445 g          311 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggt
//
LOCUS       DADLEE010000273         1413 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000273 DADLEE010000000
VERSION     DADLEE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.280"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..293
                     /locus_tag="JQH51_004336"
     CDS             <1..293
                     /locus_tag="JQH51_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY6078413.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            353..1396
                     /locus_tag="JQH51_004337"
     CDS             353..1396
                     /locus_tag="JQH51_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY6078414.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF
                     LPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGG
                     TSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQN
                     ETQQAGYDENIPV"
BASE COUNT          437 a          320 c          347 g          309 t
ORIGIN      
        1 gcgcttatcc gatttttgat cgtattgaag aaatggcatg ggctcgtcac taccagcaga
       61 tcgttcgtga agaaaaagaa acggaactgg cggacgacct ggaaaaaggt ctgcctcagc
      121 acctgtttga atcgctctgc atcgaccatt tgcaacgcca cggtgccaat aaacaggcaa
      181 tcagccacgc atttgatgat gatgttgagt ttcaggagcg catggcagaa cacatccggt
      241 acatggctga aaccattgcc caccaccagg ttgatattga tttggaggac taaaatctat
      301 ggcgcttttt caacgagcaa taaacacaca ggcctatcta aaagcaggaa ttatggggtt
      361 tgctggcgac ggcaaaacct acacagcaag cgaactggct attggtctgg taatgcttat
      421 gcggcaacgg ggaattgaag ctggtaaccg cccggtaatg tttctggaca cagaaacagg
      481 ctctgactgg gttaaacccc gctttgatgc ggaaaatatc gaattattta cggcaaaaac
      541 ccgctcgttt gttgatctgc ttgaagccat taatgaagcg gaaagcagcg gttccgtaat
      601 gatcatcgac tccatcagtc acttctggac cggattatgc gacgagtatg ccagacgccg
      661 aaaccgtaaa cgaggactgg aattttcaga ctgggcgtgg ctcaaacagg agtggcgacg
      721 ctttaccgat cgatttgtta acagtcaggc gcacattatt atgtgcgggc gtgcaggata
      781 tgagtatgac tttttcgaag gtgatgatgg caaacgccag ttggcaaaaa ccggtataaa
      841 aatgaaagca gaaacagaaa ccggttatga accctccatc cggatccaga tggagaagca
      901 aatgaacata gaaaccggtc aggtgtggcg caccgccaga atactaaaag atcgctccac
      961 ccgtattgat ggacaggtat tttcaaaccc aacatttaaa aactttcttc ctcatatcga
     1021 gtctcttaac ctgggaggtg aacaccccgg catcgatacc tcacgggata acagtgaatt
     1081 atttgccaac gacggtacac cgacatggct aaaagaaaag cgtgcaaaag aaatcgcact
     1141 tgatgaaatt atcgagttgc tcaacaagca ccatgggggc accagtaacg acgccaaacg
     1201 cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc tcatgggaaa gaatcaaagg
     1261 tatggactgg ccgacaatca aagctggtcg taatgcacta tggattgaac tggaaggcgt
     1321 tgaatatgca ttcccggatc cacaaacaca aaacgaaacg caacaagcag gatacgatga
     1381 gaatattccg gtgtaaaacc aagtgcattt tgg
//
LOCUS       DADLEE010000274         1404 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000274 DADLEE010000000
VERSION     DADLEE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.281"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..167
                     /locus_tag="JQH51_004338"
     CDS             <1..167
                     /locus_tag="JQH51_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY6078415.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="JQH51_004339"
     CDS             152..790
                     /locus_tag="JQH51_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY6078416.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="JQH51_004340"
     CDS             1018..>1404
                     /locus_tag="JQH51_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078417.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DADLEE010000275         1366 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000275 DADLEE010000000
VERSION     DADLEE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.282"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            400..1149
                     /locus_tag="JQH51_004341"
     CDS             400..1149
                     /locus_tag="JQH51_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY6078418.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DADLEE010000276         1366 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000276 DADLEE010000000
VERSION     DADLEE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.283"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..199)
                     /locus_tag="JQH51_004342"
     CDS             complement(<1..199)
                     /locus_tag="JQH51_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078419.1"
                     /translation="MPMSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAW
                     IKKYGPDSSTNKEQSDAQAEIR"
     gene            251..1276
                     /locus_tag="JQH51_004343"
                     /pseudo
     CDS             251..1276
                     /locus_tag="JQH51_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1249..>1366
                     /locus_tag="JQH51_004344"
     CDS             1249..>1366
                     /locus_tag="JQH51_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY6078420.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          372 a          333 c          320 g          341 t
ORIGIN      
        1 ggcggatctc ggcctgagca tctgactgtt ctttattagt ggaagaatcc ggaccgtact
       61 tctttatcca ggcataaagg ctgtgggtgg tgatatcgag acgtgttgca acgctggcaa
      121 cagaataacc gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac
      181 gcttaccgct catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta
      241 aacccgtggc gattcacaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt
      301 gtacaccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
      361 cctttcacaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca
      421 gcccccgcaa gatgcgataa ccgctcctga ctttttctgc ttttaccagc caggcatgac
      481 cttcgaacaa tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat
      541 gatgatcggc atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa
      601 taaacaacgt tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg
      661 gtacatttac cccatttacg actggaaagt ggctgatatc tggacctgat atgcaaatca
      721 tcaaaatctc tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca
      781 tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctgtcacgt
      841 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
      901 catttacgcc ggacatgaca atcatttcta tgggcatcgg aaaatcctca aaccagaaca
      961 tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga
     1021 gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt
     1081 tccacaaacc cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcggatatg
     1141 caaagtttta ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc
     1201 gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact
     1261 atgcaacaac gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat
     1321 cgcatcaaag cgattaatga aatccgcatg gcgatccatc aggtta
//
LOCUS       DADLEE010000277         1330 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000277 DADLEE010000000
VERSION     DADLEE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.284"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(41..100)
                     /locus_tag="JQH51_004345"
     CDS             complement(41..100)
                     /locus_tag="JQH51_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY6078421.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="JQH51_004346"
     CDS             complement(339..1247)
                     /locus_tag="JQH51_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078422.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DADLEE010000278         1277 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000278 DADLEE010000000
VERSION     DADLEE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.285"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JQH51_004347"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JQH51_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY6078423.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADLEE010000279         1232 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000279 DADLEE010000000
VERSION     DADLEE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.286"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQH51_004348"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQH51_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY6078424.1"
                     /translation="MPIMLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          362 a          207 c          297 g          366 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg gggcaatgaa
      721 aagttattat ttatcggtaa catgatgggc atccagttaa agccggtgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gt
//
LOCUS       DADLEE010000280         1199 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000280 DADLEE010000000
VERSION     DADLEE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.287"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..496
                     /locus_tag="JQH51_004349"
     CDS             <1..496
                     /locus_tag="JQH51_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY6078425.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1199
                     /locus_tag="JQH51_004350"
     CDS             560..>1199
                     /locus_tag="JQH51_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078426.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT          283 a          285 c          388 g          243 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtcagaaa
//
LOCUS       DADLEE010000281         1159 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000281 DADLEE010000000
VERSION     DADLEE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.288"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="JQH51_004351"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="JQH51_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAY6078427.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DADLEE010000282         1148 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000282 DADLEE010000000
VERSION     DADLEE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.289"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            35..738
                     /locus_tag="JQH51_004352"
                     /pseudo
     CDS             35..738
                     /locus_tag="JQH51_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709929.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(740..838)
                     /locus_tag="JQH51_004353"
     CDS             complement(740..838)
                     /locus_tag="JQH51_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY6078428.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          300 a          285 c          247 g          316 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttatcactt tggcgtcagc tggtttaacc ctgaagatct tgccactaag
      541 cgtaaagtga tgaaccgtgg actacggaaa attttgctcg cccgccacgc gttgttcgat
      601 gaagtggcct ctgccagcct cgcaccgatc tctgcatttg acgttctgat tagcgatcgt
      661 ccgttaccgg cagattatgt tacgcactgc cagaatggtt ctgtaaagat cattacacct
      721 gattcagaag acgaatgact tactgaaaaa acaccacaat cttgttaaac atcgtcgggt
      781 tggactgatt acgttgcact ttcaccacat attccagctt atctatttgg cttatcacct
      841 actccagacg ctggtcatcc ttgaccagta gccagatatg gcttttgtcg cagtcctgaa
      901 tcggcaggca gagaatgtct tcaacgttaa gagcgcgacg ggcaaaagcc cacaaaggtg
      961 agtcattaca cccggatggt aaacaggcac ttccttgttg tagaagccgt tatattgtcc
     1021 ataatgtctg ggtttttaat gcatactggg taacaaccgg acgaatcttc gaatatcact
     1081 ggcgctgcca gccggtttga attcatctca caaccctgca tagagcgaat ctcctgcctg
     1141 cacgtcac
//
LOCUS       DADLEE010000283         1100 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000283 DADLEE010000000
VERSION     DADLEE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.290"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            55..>1100
                     /locus_tag="JQH51_004354"
     CDS             55..>1100
                     /locus_tag="JQH51_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY6078429.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DADLEE010000284         1070 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000284 DADLEE010000000
VERSION     DADLEE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.291"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="JQH51_004355"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="JQH51_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY6078430.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DADLEE010000285         1069 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000285 DADLEE010000000
VERSION     DADLEE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.292"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(227..652)
                     /locus_tag="JQH51_004356"
     CDS             complement(227..652)
                     /locus_tag="JQH51_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078431.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="JQH51_004357"
     CDS             complement(649..1032)
                     /locus_tag="JQH51_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078432.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DADLEE010000286         1068 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000286 DADLEE010000000
VERSION     DADLEE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.293"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            18..1068
                     /locus_tag="JQH51_004358"
                     /pseudo
     CDS             18..1068
                     /locus_tag="JQH51_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DADLEE010000287         1066 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000287 DADLEE010000000
VERSION     DADLEE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.294"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(570..749)
                     /locus_tag="JQH51_004359"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="JQH51_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            843..>1066
                     /locus_tag="JQH51_004360"
     CDS             843..>1066
                     /locus_tag="JQH51_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078433.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DADLEE010000288         1044 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000288 DADLEE010000000
VERSION     DADLEE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.295"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..574
                     /locus_tag="JQH51_004361"
                     /pseudo
     CDS             1..574
                     /locus_tag="JQH51_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          319 a          232 c          219 g          274 t
ORIGIN      
        1 tttacgccca cagcacctaa tcaggtctgg gtcgcagatt cagttgtcca ggccttccga
       61 aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt atgcagttcg gtaaaagctc
      121 agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa gcacaggagc gaatcagcgc
      181 acttcaggag gaaatggcgg aaacactggg tgagcaatat gacccggtac tgccatcttc
      241 cctgcgccag tcttcagccc gtaaaccgtt accagcctca cttccccgtg ccccccgggt
      301 catccggccg gaagaggaat gctgtccggc ctgtggtggt gaactcagtc cactgggatg
      361 tgatgtgtca gagcagctgg agcttatcag cagcgccttt aaggttatcg aaaaacaacg
      421 ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag gcaccggtgc cttcaaaacc
      481 cattgcacgc agttatgccg gagcggggct tctggcccat gttgttaccg gaaagtatgc
      541 agaccatctg ccattatacc gccaaagtga tctactattc cacacagcta tttagctctc
      601 tttttgttga gctcccatga ttctttcatt tattgttact aattatttta tgttatcatg
      661 atgttagaac acgctctgct ctgtgtctct attttatcta tcatattctg aggattaact
      721 acataacaag gcgcaagata tatatacaaa aacaaataat accgttgcaa taattccttt
      781 tagtaaagga ggtgtgatta atttcagggg atttcagagt aaaaattgta caacatatta
      841 aaatatgttc atttcgttat aatacacaca aggaaatatt aagttagata atatattcta
      901 aataaccata gataaaaacg cacataatgg ggaatatttt atgaaaatac ctgaagcagt
      961 aaatcatatt aatgttcaga ataatattga tcttgttgat ggaaaaacga atccgaataa
     1021 agcaacaaaa gcattacaaa aaat
//
LOCUS       DADLEE010000289         1021 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000289 DADLEE010000000
VERSION     DADLEE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.296"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(50..190)
                     /locus_tag="JQH51_004362"
     CDS             complement(50..190)
                     /locus_tag="JQH51_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078434.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="JQH51_004363"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="JQH51_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY6078435.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          283 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DADLEE010000290          994 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000290 DADLEE010000000
VERSION     DADLEE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.297"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            86..>994
                     /locus_tag="JQH51_004364"
     CDS             86..>994
                     /locus_tag="JQH51_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY6078436.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          207 c          231 g          255 t
ORIGIN      
        1 gggctgagga atccccagaa aacgagacaa acatagcatt atgtgatcta ctgatttgtg
       61 ccaacaattc aatgagatca ctgctatgcc tgttcataaa gtatgtcaga atttcttccg
      121 ggatgcctta gcaccatttc atcagtaccg acaaaatgcc ctgatggatg ccacaatggc
      181 gttaatcaac ggagcatctc tgacgcagac cagtatcgga cgttttttgc cgggtaatgc
      241 acaagttaag aataagatca aacgtattga tcgcctcatg ggtaatgagg cgctccatcg
      301 cgatattcca atgattttca ggaatatcac ttcaatgctg acacggcagt tatctttatg
      361 tgtaatagcc gtagactgga gtggctatcc ctcccaggag catcatgttc ttcgtgccag
      421 ccttctgtgt gacgggcgtt ccattccgtt attgagtaag gttgttcctt ctgaaaaaca
      481 aaataatcca ttgatacaac atgatttcct tgattccctt gctcaatccc tgccccccga
      541 tgccagggtt attattgtca cggatgctgg atttcagagc gcatggtttc accatattac
      601 ttctctgggc tgggatttta ttggtcggat cagaaataac gttcagtatt gcctcgataa
      661 cgcgcctgaa agatggttaa aagtgtcgga tagccctgaa tgtaaaacac cggaatatat
      721 gggggctgga aggctggtta aagaaaggaa aaaaagcatc cgggggcatt tttacaccta
      781 taagaaatcg gcaaaagggc gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa
      841 aacagacaaa gaacagagca aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa
      901 tgacttcaga gcgcgagaaa tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa
      961 ctttcgtgat gaaaagaacg ggcgcttcgg attt
//
LOCUS       DADLEE010000291          987 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000291 DADLEE010000000
VERSION     DADLEE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.298"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(24..827)
                     /gene="dkgB"
                     /locus_tag="JQH51_004365"
     CDS             complement(24..827)
                     /gene="dkgB"
                     /locus_tag="JQH51_004365"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY6078437.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          223 c          223 g          282 t
ORIGIN      
        1 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
       61 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
      121 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
      181 taaccttccc ccatagccca cgccagaatc acttgtgccg gaatcgcatt gtgtttagct
      241 gcaatacgag caataacctc atctttcagt gccttaccat aagccagcgt catataggaa
      301 gtgatatgaa taccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
      361 gagagttcaa tctggttggt agcgatgttt tcagcgccaa cagcagcaat cactttttcc
      421 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcagcccttg ttttttggct
      481 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
      541 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt cagactcggg
      601 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
      661 cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc atagatttgt
      721 gcggtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
      781 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
      841 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg gaaaagagcg
      901 taaaacgcag aggatttttg cgattctagc aataatagat atccagacgc aaaaaaacat
      961 cgagcgttaa ctcgaggctt cttaata
//
LOCUS       DADLEE010000292          967 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000292 DADLEE010000000
VERSION     DADLEE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.299"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(3..485)
                     /locus_tag="JQH51_004366"
     CDS             complement(3..485)
                     /locus_tag="JQH51_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6078438.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="JQH51_004367"
     CDS             complement(476..778)
                     /locus_tag="JQH51_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY6078439.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          236 c          195 g          242 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgc
//
LOCUS       DADLEE010000293          950 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000293 DADLEE010000000
VERSION     DADLEE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.300"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>950
                     /locus_tag="JQH51_004368"
     CDS             <1..>950
                     /locus_tag="JQH51_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HAY6078440.1"
                     /translation="HAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILLVVDQP
                     ATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHAL
                     RTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPAPERVLGPRLEHPAV
                     LDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAA
                     VVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACR
                     AFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKR"
BASE COUNT          203 a          311 c          243 g          193 t
ORIGIN      
        1 atcacgctgt tgccattaat cgttcaggta aacgcctgtt cgataaagca ttacccaacg
       61 acgaaaacaa actcaggtcg ctaatatctg acctgaaaca acatggtcag atactgctgg
      121 ttgttgatca gccagctacc atcggtgcgt tacctgtcgc cgttgcccgc tcagaaggag
      181 tccttgtcgg atacctccct ggactggcca tgcgccgcat agccgactta cacgccggtg
      241 aagctaaaac tgatgctcgt gacgctgcca tcattgccga agctgcccgt accctgcctc
      301 acgcgctacg cacgctgaaa ctggctgacg agcaaatcgc cgaactctcc atgctctgcg
      361 gcttcgatga tgatcttgcc gcacagacaa cgcaggccag caaccgtatc cgcggccttc
      421 tgacccagat acatccggca ccggagcgcg ttctcggtcc gagacttgag cacccggcgg
      481 tactcgatct tctccagcga tatccctcac cagaaaaact cgcttcgctg ggtgagaaga
      541 agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca
      601 tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag
      661 tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc
      721 ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg
      781 gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct
      841 ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcggcga tccggctcgt
      901 caatacgcgg tgagcatccc tcgcgacggg gtaataaagc tctcaaacgg
//
LOCUS       DADLEE010000294          932 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000294 DADLEE010000000
VERSION     DADLEE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.301"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            47..319
                     /locus_tag="JQH51_004369"
     CDS             47..319
                     /locus_tag="JQH51_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078441.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
BASE COUNT          245 a          181 c          216 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aa
//
LOCUS       DADLEE010000295          922 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000295 DADLEE010000000
VERSION     DADLEE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.302"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..199
                     /locus_tag="JQH51_004370"
                     /pseudo
     CDS             1..199
                     /locus_tag="JQH51_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..>922
                     /locus_tag="JQH51_004371"
     CDS             299..>922
                     /locus_tag="JQH51_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY6078442.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          205 g          208 t
ORIGIN      
        1 ttggctacgt aattaaaaca gaaaataaag aaaccccatt cactgtagtt gatatcgacg
       61 gtctatcagg aaactttaaa actcttgttg aaggtgccac aaaaatgtgc ctagtttaca
      121 tcggaaataa tctgcccgca gaaaaaaagc cgaatttctg gcaaatctga ttgcaatgaa
      181 attaaaaggt gaaatctgaa aaaaagaaag cctgcacagt gtgcaggcct gagtgaagaa
      241 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
      301 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      361 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      421 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      481 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      541 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      601 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      661 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      721 tcttctgcac atttttacgt atgctgccgg ggatctgaaa tacctgcatc atgccacccg
      781 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      841 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      901 ccgcctgact gatgcaatta ag
//
LOCUS       DADLEE010000296          901 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000296 DADLEE010000000
VERSION     DADLEE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.303"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            103..>901
                     /locus_tag="JQH51_004372"
     CDS             103..>901
                     /locus_tag="JQH51_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY6078443.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVHVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          306 a          181 c          156 g          258 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcatc
      661 cccccctttc cagataagct tgaagggctg gctctggcta ataattttat agaacaacta
      721 ccggaattac cttttagtat gaacagggct gtgctaatga ataataatct gacaacactt
      781 ccggaaagtg tcctgagatt agctcagaat gccttcgtac atgttgcagg taatccattg
      841 tctggccata ccatgcgtac actacaacaa ataaccaccg gaccagatta ttctggtcct
      901 c
//
LOCUS       DADLEE010000297          887 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000297 DADLEE010000000
VERSION     DADLEE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.304"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            270..743
                     /locus_tag="JQH51_004373"
     CDS             270..743
                     /locus_tag="JQH51_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY6078444.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          271 a          186 c          196 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacggag
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DADLEE010000298          870 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000298 DADLEE010000000
VERSION     DADLEE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.305"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>870
                     /locus_tag="JQH51_004374"
     CDS             <1..>870
                     /locus_tag="JQH51_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY6078445.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DADLEE010000299          836 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000299 DADLEE010000000
VERSION     DADLEE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.306"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(52..720)
                     /locus_tag="JQH51_004375"
                     /pseudo
     CDS             complement(52..720)
                     /locus_tag="JQH51_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
     gene            complement(710..>836)
                     /locus_tag="JQH51_004376"
     CDS             complement(710..>836)
                     /locus_tag="JQH51_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078446.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          271 a          215 c          186 g          164 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatatggcag
       61 tgctattttg attcgacgtt gatcaagttt ctgataattc caacgacggt cgacgggcac
      121 catccagtca atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg
      181 ccatgtcgtc agcgtcgaaa tggcgatgag taaaaaccat ccgccacaga ctttacgcca
      241 cctcacagcc agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag
      301 cgaaggagac tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcctgt
      361 caccccactc acttcaggac ggataaaact aaacttcagc gggtagagaa aaaaaccgca
      421 gcaggccatt accgttgcca gaataagccg gtggacaagt cggcgttgtt cgaatgtcga
      481 gctacaaacg aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt
      541 ccagggaaga aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct
      601 gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg
      661 ggccagcaac aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac
      721 gacgcaccgc catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat
      781 caaatccggc attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcatt
//
LOCUS       DADLEE010000300          833 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000300 DADLEE010000000
VERSION     DADLEE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.307"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..326
                     /locus_tag="JQH51_004377"
     CDS             <1..326
                     /locus_tag="JQH51_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY6078447.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQH51_004378"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQH51_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY6078448.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          242 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga gtg
//
LOCUS       DADLEE010000301          803 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000301 DADLEE010000000
VERSION     DADLEE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.308"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            46..803
                     /locus_tag="JQH51_004379"
                     /pseudo
     CDS             46..803
                     /locus_tag="JQH51_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DADLEE010000302          759 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000302 DADLEE010000000
VERSION     DADLEE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.309"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>759
                     /locus_tag="JQH51_004380"
     CDS             <1..>759
                     /locus_tag="JQH51_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078449.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DADLEE010000303          746 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000303 DADLEE010000000
VERSION     DADLEE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.310"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            42..>746
                     /locus_tag="JQH51_004381"
     CDS             42..>746
                     /locus_tag="JQH51_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078450.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVN"
BASE COUNT          164 a          199 c          221 g          162 t
ORIGIN      
        1 ggcacggttc actggccgga cggagtcgcc ccgcccttct gatgctcccc cgttttgccg
       61 atatttttca gcagggaaac cgctggctta actggctgga gaaacaaccg gaaggggcag
      121 tccgtccggt ggtaattgaa tcagtgacaa aaatcatggc ctgcgggacc acactgatgg
      181 gctacacgca gtggtgttgt tcatcaccgg actgttgcca tacaaaaaag gtctgcttcc
      241 ggtgtaaaag ccgctcctgc ccgcactgcg gggtgaaggc tggcgcacag tggatacagt
      301 atctgctgag cctggtcccc tactgcccgt ggcagcatat tgtgttcaca cttccctgcc
      361 agtactggtc cctggtgttc cacaaccggt ggttactggc agagatgagc cgcattgcag
      421 cggatgtgat actggaaatc tgccatcagg cagatgtgga gccggggata ttcacggtga
      481 tccacacatg ggggcgtgac cagcagtggc atccgcatat ccatttatcg acaactgccg
      541 gtggtgtgac gtcgggccac acctggaaaa atcttcattt ttacgcccgt aaggtgatga
      601 gcatgtggcg ctaccggata acacggttgc tgtcacggaa ataccctgac ctggtgatgc
      661 cggatgcgct ggcagcagaa ggaagcagta aacgggaatg gaatcgcttc ctggacacgc
      721 attaccgccg tggctggaat gtcaac
//
LOCUS       DADLEE010000304          745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000304 DADLEE010000000
VERSION     DADLEE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.311"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>745
                     /locus_tag="JQH51_004382"
     rRNA            <1..>745
                     /locus_tag="JQH51_004382"
                     /product="16S ribosomal RNA"
BASE COUNT          182 a          171 c          240 g          152 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggtgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaat
//
LOCUS       DADLEE010000305          730 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000305 DADLEE010000000
VERSION     DADLEE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.312"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(24..100)
                     /locus_tag="JQH51_004383"
     tRNA            complement(24..100)
                     /locus_tag="JQH51_004383"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Ile,seq:cat)
     gene            complement(290..712)
                     /locus_tag="JQH51_004384"
     CDS             complement(290..712)
                     /locus_tag="JQH51_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY6078451.1"
                     /translation="MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGK
                     QKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLNDAHPK
                     WSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          184 a          199 c          139 g          208 t
ORIGIN      
        1 tatccggatg agctagtggt ggttggtggc ccttgctgga cttgaaccag cgacctggcg
       61 attatgagtc gctcgctctg accaactgag ctaaagggcc ggaggcagaa taataaccat
      121 atgtcatcac atctgcaaac tcatctgatc accagcacgt ttaacgtcct gtgccgtttt
      181 tcaggtataa aaaaaaccgc ataaagcggg ctctttcaaa tgtccatgtc tgctattcgc
      241 ctcgcggtac agctttgcga agcgtacaag aattgaagca gtttttacgt caaaaagcaa
      301 taactttttt ctctatacca aaagccataa ccattggttt gtacaaaata aattctgcca
      361 cctttagcca atgctcaatg cgtctttcac aggttcttaa actccatttc gggtgtgcat
      421 cattcagcag ttcagccatt ttgcatttgg tcatcccccg cccctcatac cgttgccgaa
      481 ggacgctaat caatcctgga tgctctgcca gcacctcact tatgacccga tcaatacata
      541 acgcctctgc atcagtacaa tgcgccagcc agctcttttg cttaccgtta atcatatccc
      601 gcaaaaaagc ctcaagttca gacttgttca gacctgcttt tttcatcctc cggagcgcct
      661 cgttaattgc cgtttttgtc agctttttag aggtcaacat caggttaaac atattccccg
      721 tcttaccgcc
//
LOCUS       DADLEE010000306          704 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000306 DADLEE010000000
VERSION     DADLEE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.313"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..443)
                     /locus_tag="JQH51_004385"
     CDS             complement(<1..443)
                     /locus_tag="JQH51_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY6078452.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DADLEE010000307          694 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000307 DADLEE010000000
VERSION     DADLEE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.314"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            32..493
                     /locus_tag="JQH51_004386"
     CDS             32..493
                     /locus_tag="JQH51_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY6078453.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="JQH51_004387"
     CDS             complement(534..>694)
                     /locus_tag="JQH51_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY6078454.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          152 c          159 g          179 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DADLEE010000308          693 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000308 DADLEE010000000
VERSION     DADLEE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.315"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..525
                     /locus_tag="JQH51_004388"
                     /pseudo
     CDS             1..525
                     /locus_tag="JQH51_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            589..>693
                     /locus_tag="JQH51_004389"
     CDS             589..>693
                     /locus_tag="JQH51_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6078455.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DADLEE010000309          685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000309 DADLEE010000000
VERSION     DADLEE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.316"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            52..685
                     /locus_tag="JQH51_004390"
                     /pseudo
     CDS             52..685
                     /locus_tag="JQH51_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DADLEE010000310          656 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000310 DADLEE010000000
VERSION     DADLEE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.317"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(151..648)
                     /locus_tag="JQH51_004391"
     CDS             complement(151..648)
                     /locus_tag="JQH51_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY6078456.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DADLEE010000311          644 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000311 DADLEE010000000
VERSION     DADLEE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.318"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..219
                     /locus_tag="JQH51_004392"
     CDS             1..219
                     /locus_tag="JQH51_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY6078457.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDYAVNDG"
     gene            complement(270..>644)
                     /locus_tag="JQH51_004393"
     CDS             complement(270..>644)
                     /locus_tag="JQH51_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY6078458.1"
                     /translation="DAYADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFS
                     QEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHIVQI
                     KGESYRLRQKRKAGVIAEANPE"
BASE COUNT          162 a          150 c          160 g          172 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtc
//
LOCUS       DADLEE010000312          636 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000312 DADLEE010000000
VERSION     DADLEE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.319"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            88..153
                     /locus_tag="JQH51_004394"
                     /pseudo
     CDS             88..153
                     /locus_tag="JQH51_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JQH51_004395"
     CDS             381..629
                     /locus_tag="JQH51_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY6078459.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLEE010000313          623 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000313 DADLEE010000000
VERSION     DADLEE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.320"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..251)
                     /locus_tag="JQH51_004396"
     CDS             complement(<1..251)
                     /locus_tag="JQH51_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY6078460.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="JQH51_004397"
     CDS             complement(272..>623)
                     /locus_tag="JQH51_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6078461.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DADLEE010000314          586 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000314 DADLEE010000000
VERSION     DADLEE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.321"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            75..586
                     /locus_tag="JQH51_004398"
                     /pseudo
     CDS             75..586
                     /locus_tag="JQH51_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          123 a          154 c          166 g          143 t
ORIGIN      
        1 gcactaagca cataattgcg gtgatgctgc caacttactg atttagtgta tgatggtgtt
       61 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
      121 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc
      181 gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa
      241 tcattgatat ggccatgaat ggcgttggat gccgggcaac cgcccgcatt atgggcgttg
      301 gcctcaacac gattttccgc catttaaaaa actcaggccg cagtcggtaa cctcgcgcat
      361 acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gatacgtcgg
      421 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac aggctccgga agacggttgt
      481 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc
      541 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtat
//
LOCUS       DADLEE010000315          569 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000315 DADLEE010000000
VERSION     DADLEE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.322"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            155..>569
                     /locus_tag="JQH51_004399"
     CDS             155..>569
                     /locus_tag="JQH51_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY6078462.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          143 a          156 c          144 g          126 t
ORIGIN      
        1 gggctgcatc cgactctttt atcaacgctt acgtaattaa gggacacagc ctaaatagct
       61 gcgcggaata gtagatccct ttgagggaac ttagcccgga ttgtgcgatc tgatcaatcg
      121 ccaaatcaaa acaaatcacc aaccggactg agcaatgccg atcatagcac caatttcccg
      181 tgacgaacga cgcctgatgc agaaagccat ccataaaaca cacgataaaa attatgcccg
      241 cagactgact gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgccagaac
      301 gctctgctgc gcccgttcct ctgttggacg ctggattaac tggttcacgc agtcgggtgt
      361 tgagggactg aaatcattac ctgccgggcg tgcccgtcgc tggccgtttg agcatatctg
      421 cacactgtta cgtgagctgg taaaacattc tcccggcgac tttggctacc agcgttcacg
      481 ctggagtaca gaactgctgg caataaaaat caatgagata accggctgcc agttaaatgc
      541 cggaaccgtt cgccgctggt tgccgtctg
//
LOCUS       DADLEE010000316          566 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000316 DADLEE010000000
VERSION     DADLEE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.323"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..297
                     /locus_tag="JQH51_004400"
     CDS             <1..297
                     /locus_tag="JQH51_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY6078463.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JQH51_004401"
     CDS             complement(405..>566)
                     /locus_tag="JQH51_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078464.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLEE010000317          563 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000317 DADLEE010000000
VERSION     DADLEE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.324"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..485)
                     /locus_tag="JQH51_004402"
     CDS             complement(<1..485)
                     /locus_tag="JQH51_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY6078465.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
BASE COUNT          142 a          135 c          131 g          155 t
ORIGIN      
        1 accggaccgc acaccaggtt tggtttatcc gttggtttgc ctgcagcttc catacgcttg
       61 cgccaacgcc atttcatcgc catcccgccg agcatacagg cctcggaaga accaatggtg
      121 ttggtgccaa cggcctgacc atttttcggc gcaggcgcat gccacagatc ggcaaccatg
      181 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
      241 ttgttaatgg ataaatccat caacttgtgg acgttttcgt cgtcccaggt ctggcagaaa
      301 gtggccaggt tctgacgagc gttgccatca agatataatt catcattgat aatctgaaat
      361 gcgacatcgt cgcgcatttc gtgcagcgga aaacgttttg attctgcgat agtggaaata
      421 gacttcgcac caaaacgtga atcgagtagt tccgacctta aatccgttac ttgcttctta
      481 tccattttaa actccttaaa atgattggat cgcattaaaa aagtaggatt tatcgataaa
      541 gtaagcaagt gcaaaggact cgt
//
LOCUS       DADLEE010000318          557 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000318 DADLEE010000000
VERSION     DADLEE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.325"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>557
                     /locus_tag="JQH51_004403"
     CDS             <1..>557
                     /locus_tag="JQH51_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY6078466.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          164 a          137 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagcc
//
LOCUS       DADLEE010000319          551 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000319 DADLEE010000000
VERSION     DADLEE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.326"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>551)
                     /locus_tag="JQH51_004404"
     CDS             complement(<1..>551)
                     /locus_tag="JQH51_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078467.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DADLEE010000320          550 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000320 DADLEE010000000
VERSION     DADLEE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.327"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="JQH51_004405"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="JQH51_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY6078468.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..>550)
                     /gene="csgA"
                     /locus_tag="JQH51_004406"
     CDS             complement(202..>550)
                     /gene="csgA"
                     /locus_tag="JQH51_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY6078469.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          146 a          127 c          132 g          145 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta
//
LOCUS       DADLEE010000321          541 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000321 DADLEE010000000
VERSION     DADLEE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.328"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            390..>541
                     /locus_tag="JQH51_004407"
     CDS             390..>541
                     /locus_tag="JQH51_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078470.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          137 a          143 c          119 g          142 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagag accctagaga gaccctagag tggctcctag agagaccctc ccagtagctc
      241 cgcacagcta catcgtcatc atcatagtat tcaatctcaa agtcattctg tttgcatcct
      301 gttgtcctgc tggtctccct tcgcttgggg actttgagta ggtcagtggg ggtgcgactt
      361 cagggtgcaa acaaaaagga aatcaagcga tgtctcaaca accacaacaa ccacaacaag
      421 aaatcagcat cattaacctc gaccagcttg tgtccatgac ctcagttgaa attgctgagc
      481 tgacaggcaa ggagcaccga aatgtccttc gtgatattcg taagatgctc gaagaactta
      541 a
//
LOCUS       DADLEE010000322          533 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000322 DADLEE010000000
VERSION     DADLEE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.329"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
BASE COUNT          173 a           78 c          107 g          175 t
ORIGIN      
        1 ttatatgaat atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa
       61 atagccctgc tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg
      121 tatctgtcag cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag
      181 caatgtatct tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc
      241 ttacatcacg actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc
      301 acaccgtaat gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc
      361 tcttattttc cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga
      421 tctcatttcg agtattgaga aatttgtgat ttttaaattt ttcaataagc cctgtaaggg
      481 attggggttt tatgagaaat aaagagacgt attgtctttg tttaaacggt acg
//
LOCUS       DADLEE010000323          508 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000323 DADLEE010000000
VERSION     DADLEE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.330"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(47..442)
                     /locus_tag="JQH51_004408"
     CDS             complement(47..442)
                     /locus_tag="JQH51_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078471.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          148 a          122 c          108 g          130 t
ORIGIN      
        1 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcat
       61 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
      121 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      181 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      241 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      301 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      361 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      421 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      481 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DADLEE010000324          483 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000324 DADLEE010000000
VERSION     DADLEE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.331"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..483
                     /locus_tag="JQH51_004409"
                     /pseudo
     CDS             1..483
                     /locus_tag="JQH51_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           95 g          130 t
ORIGIN      
        1 tcgagtaata atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa
       61 gtaccagaaa gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc
      121 ttactcaacg cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt
      181 tatggcaaag atatggtgac aaagtccggg gatatattga ttgatataca attaaataag
      241 gatggcagtc tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat
      301 aatattgacc ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa
      361 tcctggtgac gttattagcc tgtatggcac tttagataac attccccttg tagttaagga
      421 atgcacgggt aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga
      481 aaa
//
LOCUS       DADLEE010000325          473 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000325 DADLEE010000000
VERSION     DADLEE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.332"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..473
                     /locus_tag="JQH51_004410"
                     /pseudo
     CDS             1..473
                     /locus_tag="JQH51_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DADLEE010000326          472 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000326 DADLEE010000000
VERSION     DADLEE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.333"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>472
                     /locus_tag="JQH51_004411"
     CDS             <1..>472
                     /locus_tag="JQH51_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078472.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DADLEE010000327          471 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000327 DADLEE010000000
VERSION     DADLEE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.334"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..361
                     /locus_tag="JQH51_004412"
     CDS             <1..361
                     /locus_tag="JQH51_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY6078473.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DADLEE010000328          463 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000328 DADLEE010000000
VERSION     DADLEE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.335"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>463
                     /locus_tag="JQH51_004413"
     CDS             <1..>463
                     /locus_tag="JQH51_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078474.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DADLEE010000329          459 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000329 DADLEE010000000
VERSION     DADLEE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.336"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>459)
                     /locus_tag="JQH51_004414"
     CDS             complement(<1..>459)
                     /locus_tag="JQH51_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY6078475.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DADLEE010000330          443 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000330 DADLEE010000000
VERSION     DADLEE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.337"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            213..>443
                     /locus_tag="JQH51_004415"
     CDS             213..>443
                     /locus_tag="JQH51_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY6078476.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DADLEE010000331          439 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000331 DADLEE010000000
VERSION     DADLEE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.338"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..269
                     /locus_tag="JQH51_004416"
     CDS             <1..269
                     /locus_tag="JQH51_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6078477.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>439
                     /gene="tnpB"
                     /locus_tag="JQH51_004417"
     CDS             266..>439
                     /gene="tnpB"
                     /locus_tag="JQH51_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6078478.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILW"
BASE COUNT          109 a          114 c          125 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtgg
//
LOCUS       DADLEE010000332          434 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000332 DADLEE010000000
VERSION     DADLEE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.339"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            35..434
                     /locus_tag="JQH51_004418"
                     /pseudo
     CDS             35..434
                     /locus_tag="JQH51_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DADLEE010000333          410 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000333 DADLEE010000000
VERSION     DADLEE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.340"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..120
                     /locus_tag="JQH51_004419"
     CDS             <1..120
                     /locus_tag="JQH51_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAY6078479.1"
                     /translation="ERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            117..>410
                     /locus_tag="JQH51_004420"
     CDS             117..>410
                     /locus_tag="JQH51_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY6078480.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKA"
BASE COUNT          100 a          113 c          121 g           76 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc
//
LOCUS       DADLEE010000334          388 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000334 DADLEE010000000
VERSION     DADLEE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.341"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(40..>388)
                     /locus_tag="JQH51_004421"
     CDS             complement(40..>388)
                     /locus_tag="JQH51_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY6078481.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           89 c           87 g          120 t
ORIGIN      
        1 ttcgtgaagc tgttgaaggg tggattctta acggtggcgt taatcagaaa tgctcctgat
       61 gtgccagaac aatttatcag cgtgaagctc atacgctttg cgctgagcct ctatccagtc
      121 gtgtttattg tatacggaca ttatcccgcc aagttcatga ccgagcattc gctcagtaac
      181 atgaggctcg acattcatct cggacagccg tgtaacaagc gttcttctga aatcatgtgt
      241 tcgccagtaa cctagatcca tccctcccct tattctattt acgaatcggt tggctgcagc
      301 gatgctgatc ggtttgttta tgtcttctcc agggaaaaga acatcattgt acattgtcat
      361 ggccttttcg agaaaaggct taatttgt
//
LOCUS       DADLEE010000335          382 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000335 DADLEE010000000
VERSION     DADLEE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.342"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(1..250)
                     /locus_tag="JQH51_004422"
                     /pseudo
     CDS             complement(1..250)
                     /locus_tag="JQH51_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT           88 a          122 c           86 g           86 t
ORIGIN      
        1 cactgtctcc gttatggcgt acaccacatc ttccagtaat cgccgcttca ccggactcag
       61 gaccccgtat caccatccgg aaccccttat ccgccacatg ccaggagaac ctttccataa
      121 actcgtcgcc actcatcaca aggttttctt ctcgttttgt ccggtggctg ttgtaacgca
      181 ggccaatttc atcctgaccg gcataatgtt ccaggcggct catctgcacc ggctgcttct
      241 ccagccagtt aagccatcgg tttccctgct gaaaaatatc ggcaaaacgg ggaagcatca
      301 gaaaggcgga ataacgccat ccggccagtg aaccgtaccg cattcagggc aatacatgcc
      361 gtcggcgttg ataccggcag ga
//
LOCUS       DADLEE010000336          381 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000336 DADLEE010000000
VERSION     DADLEE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.343"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(273..>381)
                     /locus_tag="JQH51_004423"
     CDS             complement(273..>381)
                     /locus_tag="JQH51_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY6078482.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DADLEE010000337          374 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000337 DADLEE010000000
VERSION     DADLEE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.344"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>374
                     /locus_tag="JQH51_004424"
     CDS             <1..>374
                     /locus_tag="JQH51_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6078483.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DADLEE010000338          372 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000338 DADLEE010000000
VERSION     DADLEE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.345"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..311)
                     /locus_tag="JQH51_004425"
     CDS             complement(<1..311)
                     /locus_tag="JQH51_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY6078484.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DADLEE010000339          369 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000339 DADLEE010000000
VERSION     DADLEE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.346"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>369
                     /locus_tag="JQH51_004426"
     CDS             <1..>369
                     /locus_tag="JQH51_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY6078485.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYI"
BASE COUNT           80 a           94 c          111 g           84 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacatt
//
LOCUS       DADLEE010000340          367 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000340 DADLEE010000000
VERSION     DADLEE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.347"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(35..307)
                     /locus_tag="JQH51_004427"
     CDS             complement(35..307)
                     /locus_tag="JQH51_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078486.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           84 a           85 c           98 g          100 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcag
//
LOCUS       DADLEE010000341          364 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000341 DADLEE010000000
VERSION     DADLEE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.348"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..364
                     /locus_tag="JQH51_004428"
                     /pseudo
     CDS             1..364
                     /locus_tag="JQH51_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT           89 a           90 c           98 g           87 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acgg
//
LOCUS       DADLEE010000342          357 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000342 DADLEE010000000
VERSION     DADLEE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.349"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            39..>357
                     /locus_tag="JQH51_004429"
     CDS             39..>357
                     /locus_tag="JQH51_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY6078487.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DADLEE010000343          353 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000343 DADLEE010000000
VERSION     DADLEE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.350"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..264
                     /locus_tag="JQH51_004430"
     CDS             <1..264
                     /locus_tag="JQH51_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY6078488.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="JQH51_004431"
     CDS             288..>353
                     /locus_tag="JQH51_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078489.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DADLEE010000344          351 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000344 DADLEE010000000
VERSION     DADLEE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.351"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            1..351
                     /locus_tag="JQH51_004432"
                     /pseudo
     CDS             1..351
                     /locus_tag="JQH51_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          104 a           77 c           91 g           79 t
ORIGIN      
        1 aagccgtcaa aacggttcct gaaaatcagc tttcgatcag tgaaggagct tcccgattat
       61 ctgttcctga aggcacttta ggacaatggg ttaccgccgc aagaaaaggg ctcaatacac
      121 caggctcccg cacagtggct gagctggaat ctgaagttat gcaactgcgt aaggctttaa
      181 atgaagcacg acttgagcga gatatattaa aaaaagcaac agcgtatttt gcacaggagt
      241 cgctgaaaaa tacgcgttag tcgaacaatg gcgacaacaa ttccctgttg aagcgatgtg
      301 tcgggtacct ggtgtatcca ggagcggtta ttacgaccgg gtacagcatg c
//
LOCUS       DADLEE010000345          349 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000345 DADLEE010000000
VERSION     DADLEE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.352"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>349
                     /locus_tag="JQH51_004433"
     CDS             <1..>349
                     /locus_tag="JQH51_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY6078490.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDR"
BASE COUNT           80 a           88 c          102 g           79 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgt
//
LOCUS       DADLEE010000346          344 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000346 DADLEE010000000
VERSION     DADLEE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.353"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            31..318
                     /locus_tag="JQH51_004434"
     CDS             31..318
                     /locus_tag="JQH51_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY6078491.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          102 a           72 c           86 g           84 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tact
//
LOCUS       DADLEE010000347          341 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000347 DADLEE010000000
VERSION     DADLEE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.354"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>341
                     /locus_tag="JQH51_004435"
     rRNA            <1..>341
                     /locus_tag="JQH51_004435"
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           76 c          114 g           64 t
ORIGIN      
        1 ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta
       61 ataccgcata acgtcgcaag accaaagagg gggaccttcg ggcctcttgc catcggatgt
      121 gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg atccctagct
      181 ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg
      241 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga
      301 agaaggcctt cgggttgtaa agtactttca gcggggagga a
//
LOCUS       DADLEE010000348          340 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000348 DADLEE010000000
VERSION     DADLEE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.355"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(<1..>340)
                     /locus_tag="JQH51_004436"
     CDS             complement(<1..>340)
                     /locus_tag="JQH51_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY6078492.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DADLEE010000349          339 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000349 DADLEE010000000
VERSION     DADLEE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.356"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..170
                     /gene="xisR"
                     /locus_tag="JQH51_004437"
     CDS             <1..170
                     /gene="xisR"
                     /locus_tag="JQH51_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY6078493.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>339
                     /locus_tag="JQH51_004438"
     CDS             172..>339
                     /locus_tag="JQH51_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAY6078494.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           63 c           82 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aagcgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttcc
//
LOCUS       DADLEE010000350          335 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000350 DADLEE010000000
VERSION     DADLEE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.357"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..>335
                     /locus_tag="JQH51_004439"
     CDS             <1..>335
                     /locus_tag="JQH51_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY6078495.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DADLEE010000351          333 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000351 DADLEE010000000
VERSION     DADLEE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.358"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(43..330)
                     /locus_tag="JQH51_004440"
     CDS             complement(43..330)
                     /locus_tag="JQH51_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY6078496.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgt
//
LOCUS       DADLEE010000352          332 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000352 DADLEE010000000
VERSION     DADLEE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.359"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            51..>332
                     /locus_tag="JQH51_004441"
     CDS             51..>332
                     /locus_tag="JQH51_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY6078497.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DADLEE010000353          327 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000353 DADLEE010000000
VERSION     DADLEE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.360"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
BASE COUNT           73 a           96 c           76 g           82 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc
      121 cccttcctcg ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga
      181 gcgccggacc ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc
      241 aaaacgctgc aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc
      301 ccggcttttt gggccaggga tatgtgt
//
LOCUS       DADLEE010000354          325 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000354 DADLEE010000000
VERSION     DADLEE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.361"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
BASE COUNT           70 a           92 c           79 g           84 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaa
//
LOCUS       DADLEE010000355          301 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000355 DADLEE010000000
VERSION     DADLEE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.denovo.362"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            61..>301
                     /locus_tag="JQH51_004442"
     CDS             61..>301
                     /locus_tag="JQH51_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAY6078498.1"
                     /translation="MAELAIELDDMTIITEKLRDMKNALSKLNHDIEEDTKRIKAITT
                     GSLKDIDRTTKEGMVETQLLIKGVLKEIVIDAAKRR"
BASE COUNT          117 a           55 c           64 g           65 t
ORIGIN      
        1 actgacacta ttgatagcct taaattacag cgtgaagatt tgcagcgtaa aatctcaaaa
       61 atggcagaac ttgctattga actcgatgat atgacaatta tcacagagaa attacgcgac
      121 atgaagaatg cgctttcaaa actcaatcat gatatcgaag aggatacaaa gcggataaag
      181 gccatcacaa ctggaagcct caaagatatt gacaggacaa caaaggaagg gatggtagag
      241 acacagctac ttatcaaggg cgtactcaaa gaaatagtta tagatgccgc taaacgtcga
      301 t
//
LOCUS       DADLEE010000356         9713 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.130, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000356 DADLEE010000000
VERSION     DADLEE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.130"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            complement(17..205)
                     /locus_tag="JQH51_004443"
     CDS             complement(17..205)
                     /locus_tag="JQH51_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY6078499.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="JQH51_004444"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="JQH51_004444"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY6078500.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="JQH51_004445"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="JQH51_004445"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY6078501.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /locus_tag="JQH51_004446"
     CDS             3527..4261
                     /locus_tag="JQH51_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY6078502.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="JQH51_004447"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="JQH51_004447"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY6078503.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="JQH51_004448"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="JQH51_004448"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY6078504.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /gene="blaEC"
                     /locus_tag="JQH51_004449"
     CDS             5100..6233
                     /gene="blaEC"
                     /locus_tag="JQH51_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY6078505.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /locus_tag="JQH51_004450"
     CDS             6322..6855
                     /locus_tag="JQH51_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY6078506.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /locus_tag="JQH51_004451"
     CDS             complement(6852..7169)
                     /locus_tag="JQH51_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY6078507.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="JQH51_004452"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="JQH51_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY6078508.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="JQH51_004453"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="JQH51_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY6078509.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="JQH51_004454"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="JQH51_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY6078510.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..9415
                     /gene="epmB"
                     /locus_tag="JQH51_004455"
     CDS             8387..9415
                     /gene="epmB"
                     /locus_tag="JQH51_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY6078511.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2485 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
     8881 cgatccgctg atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat
     8941 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
     9001 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
     9061 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
     9121 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
     9181 aacgttggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
     9241 tgtgctagat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
     9301 gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga
     9361 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
     9421 taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
     9481 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
     9541 tccggcaaga gcaacaaacg caagtacagc aaccactgag aaatttaatc tttgcacgtt
     9601 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     9661 taattaacct ttcttttcgg taatgactcc aacttactga tagtgtttta tgt
//
LOCUS       DADLEE010000357         8792 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.142, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000357 DADLEE010000000
VERSION     DADLEE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.142"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            263..922
                     /locus_tag="JQH51_004456"
     CDS             263..922
                     /locus_tag="JQH51_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY6078512.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="JQH51_004457"
     CDS             complement(1123..1500)
                     /locus_tag="JQH51_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6078513.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="JQH51_004458"
     CDS             complement(1567..4533)
                     /locus_tag="JQH51_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY6078514.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="JQH51_004459"
     CDS             complement(4536..5096)
                     /locus_tag="JQH51_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6078515.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="JQH51_004460"
     CDS             complement(5222..5572)
                     /locus_tag="JQH51_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY6078516.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="JQH51_004461"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="JQH51_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY6078517.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="JQH51_004462"
     CDS             6998..7828
                     /locus_tag="JQH51_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY6078518.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8732
                     /gene="aadA1"
                     /locus_tag="JQH51_004463"
     CDS             7941..8732
                     /gene="aadA1"
                     /locus_tag="JQH51_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY6078519.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2000 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8641 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     8701 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     8761 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DADLEE010000358         8789 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.143, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000358 DADLEE010000000
VERSION     DADLEE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.143"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            263..922
                     /locus_tag="JQH51_004464"
     CDS             263..922
                     /locus_tag="JQH51_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY6078520.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="JQH51_004465"
     CDS             complement(1123..1500)
                     /locus_tag="JQH51_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY6078521.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..4533)
                     /locus_tag="JQH51_004466"
     CDS             complement(1567..4533)
                     /locus_tag="JQH51_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY6078522.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4536..5096)
                     /locus_tag="JQH51_004467"
     CDS             complement(4536..5096)
                     /locus_tag="JQH51_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY6078523.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="JQH51_004468"
     CDS             complement(5222..5572)
                     /locus_tag="JQH51_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY6078524.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="JQH51_004469"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="JQH51_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY6078525.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="JQH51_004470"
     CDS             6998..7828
                     /locus_tag="JQH51_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY6078526.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8729
                     /gene="aadA1"
                     /locus_tag="JQH51_004471"
     CDS             7941..8729
                     /gene="aadA1"
                     /locus_tag="JQH51_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY6078527.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1997 a         2357 c         2482 g         1953 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     8641 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     8701 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     8761 tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DADLEE010000359         7053 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.164, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000359 DADLEE010000000
VERSION     DADLEE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.164"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            59..1102
                     /locus_tag="JQH51_004472"
     CDS             59..1102
                     /locus_tag="JQH51_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY6078528.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1218..1778
                     /locus_tag="JQH51_004473"
                     /pseudo
     CDS             1218..1778
                     /locus_tag="JQH51_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="JQH51_004474"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="JQH51_004474"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY6078529.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="JQH51_004475"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="JQH51_004475"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY6078530.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="JQH51_004476"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="JQH51_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY6078531.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /locus_tag="JQH51_004477"
     CDS             5406..5807
                     /locus_tag="JQH51_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY6078532.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="JQH51_004478"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="JQH51_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY6078533.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DADLEE010000360         4295 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.197, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000360 DADLEE010000000
VERSION     DADLEE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.197"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            558..797
                     /locus_tag="JQH51_004479"
     CDS             558..797
                     /locus_tag="JQH51_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY6078534.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="JQH51_004480"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="JQH51_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="JQH51_004481"
     CDS             2294..2767
                     /locus_tag="JQH51_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY6078535.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="JQH51_004482"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="JQH51_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY6078536.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4235
                     /gene="aadA1"
                     /locus_tag="JQH51_004483"
     CDS             3444..4235
                     /gene="aadA1"
                     /locus_tag="JQH51_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY6078537.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DADLEE010000361         4292 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.198, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000361 DADLEE010000000
VERSION     DADLEE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.198"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            558..797
                     /locus_tag="JQH51_004484"
     CDS             558..797
                     /locus_tag="JQH51_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY6078538.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="JQH51_004485"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="JQH51_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /locus_tag="JQH51_004486"
     CDS             2294..2767
                     /locus_tag="JQH51_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY6078539.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="JQH51_004487"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="JQH51_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY6078540.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4232
                     /gene="aadA1"
                     /locus_tag="JQH51_004488"
     CDS             3444..4232
                     /gene="aadA1"
                     /locus_tag="JQH51_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY6078541.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DADLEE010000362         3264 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri 2a isolate MH160088
            SAMEA55381918-rid11426403.guided.217, whole genome shotgun
            sequence.
ACCESSION   DADLEE010000362 DADLEE010000000
VERSION     DADLEE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA55381918
            Sequence Read Archive: ERR1810612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943536.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943536.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:02:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,418
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 383
            CDSs (without protein)            :: 383
            Pseudo Genes (ambiguous residues) :: 0 of 383
            Pseudo Genes (frameshifted)       :: 173 of 383
            Pseudo Genes (incomplete)         :: 97 of 383
            Pseudo Genes (internal stop)      :: 147 of 383
            Pseudo Genes (multiple problems)  :: 33 of 383
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA55381918-rid11426403.guided.217"
                     /isolate="MH160088"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /country="Ireland"
                     /collection_date="2016"
     gene            <1..124
                     /locus_tag="JQH51_004489"
     CDS             <1..124
                     /locus_tag="JQH51_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAY6078542.1"
                     /translation="VGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(102..725)
                     /gene="tetR(B)"
                     /locus_tag="JQH51_004490"
     CDS             complement(102..725)
                     /gene="tetR(B)"
                     /locus_tag="JQH51_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY6078543.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            807..2012
                     /gene="tet(B)"
                     /locus_tag="JQH51_004491"
     CDS             807..2012
                     /gene="tet(B)"
                     /locus_tag="JQH51_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY6078544.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2125..2718)
                     /gene="tetC"
                     /locus_tag="JQH51_004492"
     CDS             complement(2125..2718)
                     /gene="tetC"
                     /locus_tag="JQH51_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY6078545.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2806..3216
                     /locus_tag="JQH51_004493"
                     /pseudo
     CDS             2806..3216
                     /locus_tag="JQH51_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          883 a          595 c          672 g         1114 t
ORIGIN      
        1 cgttggagcc gcattatttt cgctttatga atctaaaggg tggttaactc gacatcttgg
       61 ttaccgtgaa gttaccatca cggaaaaagg ttatgctgct tttaagaccc actttcacat
      121 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
      181 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
      241 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
      301 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
      361 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
      421 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
      481 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
      541 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
      601 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
      661 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta
      721 gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta ttttaccact
      781 ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca ttggtaatta
      841 cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg ttattacgtg
      901 aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca ctttatgcgt
      961 taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt ggtcggcgcc
     1021 cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg gctttttcaa
     1081 gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga gctactgggg
     1141 ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg aagtggttcg
     1201 gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt ggtggttttg
     1261 caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat attgtcgctt
     1321 tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat acagataccg
     1381 aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa acgatgccca
     1441 ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca acggtgtggg
     1501 tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt tcattagcgg
     1561 gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata gccactaaat
     1621 ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca tttgcctttt
     1681 tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg gctggtggtg
     1741 ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt catcagcaag
     1801 gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt ggcccattac
     1861 tgtttgctgt tatttataat cattcactac caatttggga tggctggatt tggattattg
     1921 gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta acccctcaag
     1981 ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg atgttattcc
     2041 gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata aagaagattt
     2101 agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa agccgcggta
     2161 aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg catgtattgc
     2221 gatatgcata aaccagccat tgagtaagtt tttaagcaca tcatcatcat aagctttaag
     2281 ttggttctct tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa tgtattcaag
     2341 gctaattttt tcaagttcat tccaaccaat gataggcatc acttcttgga tagggataag
     2401 gtttttatta ttatcaataa tataatcaag ataatgttca aatatacttt ctaaggcaga
     2461 ccaaccattt gttaaatcag tttttgttgt gatgtaggca tcaatcataa ttaattgctg
     2521 cttataacag gcactgagta attgtttttt atttttaaag tgatgataaa aggcaccttt
     2581 ggtcaccaac gcttttcccg agatctcatc tattgaaaca gcttgatagc ctttttcaac
     2641 aaacaatatt cgtgctgagt taaccagtga ttgataggta ctcttaaaat tttcttgttg
     2701 atgattttta ttttccatga tagatttaaa ataacatacc gtcagtatgt ttatggtatc
     2761 atgatgatgt ggtcgtgaca atcttaagaa catttaggtt attttatgta tattgaacag
     2821 cattctcgct atcaaaataa agctaataac atccaattaa gatatgatga taagcagttt
     2881 catacaacgg ttatcaaaga tgttctatta tggattgaac ataatttaga tcagtcttta
     2941 ctgcttgatg atgtggcgaa taaagcgggt tataccaagt ggtattttca gcggctgttc
     3001 aaaaaagtaa caggggtcac actggctagc tatattcgtg ctcgtcgttt gacgaaagcg
     3061 gctgttgagt tgaggttgac gaaaaaaact atccttgaga tcgcattaaa atatcaattt
     3121 gattcccaac aatcttttac acgtcgattt aagtacattt ttaaggttac accaagttat
     3181 tatcggcgta ataaattatg ggaattggag gcaatgcact gagagggtga tgctgccaac
     3241 ttactgattt agtgtatgat ggtg
//
